chlortetracycline

naturalproductmech:mibig-8252d9cf90 MINTED SEEDED enzyme inhibitor

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	DHPRQBPJLMKORJ-RQWFMOQPSA-N
SMILES	C[C@@]1(C2C[C@H]3[C@@H](C(=O)C(=C([C@]3(C(=O)C2=C(C4=C(C=CC(=C41)Cl)O)O)O)O)C(=O)N)N(C)C)O
Stereochemistry	undefined stereocentres — the InChIKey does not distinguish this compound from its stereoisomers
Cross-references	pubchem:54676345

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Kitasatospora aureofaciens NCBITaxon:1894	SOURCE_ASSERTION	mibig:BGC0000209	PMID:23800859

Occurrences 0

None cited.

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0000209	Kitasatospora aureofaciens NCBITaxon:1894	CLUSTER_UNSTATED	genbank:HM627755.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Bioactivities 12

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

Assay	Result	Target enzyme	Source detail
Primary fluorescence-based thiol-reactive (MSTI) qHTS assay for identification of artifact compounds.	OTHER 1.6834 uM; ACTIVE	Not recorded	pubchem.aid:1845221 assay Primary fluorescence-based thiol-reactive (MSTI) qHTS assay for identification of artifact compounds. evidence reference pubchem.aid:1845221 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1845221, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 1.6834 units uM
Primary qHTS for Inhibitors of N-terminal methyltransferase 1 (NTMT1): MTaseGlo Assay	OTHER 28.1838 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1645870 assay Primary qHTS for Inhibitors of N-terminal methyltransferase 1 (NTMT1): MTaseGlo Assay evidence reference pubchem.aid:1645870 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645870, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 28.1838 units uM
Primary qHTS for Inhibitors of N-terminal methyltransferase 1 (NTMT1): MTaseGlo Assay	OTHER 28.1838 uM; NOT_DETERMINED	UniProtKB:Q9BV86	pubchem.aid:1645870 assay Primary qHTS for Inhibitors of N-terminal methyltransferase 1 (NTMT1): MTaseGlo Assay evidence reference pubchem.aid:1645870 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645870, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 28.1838 units uM target enzyme UniProtKB:Q9BV86
Primary qHTS for small molecule compounds that upregulate eGFP-tagged delta133p53alpha (in N-terminal) cells.	OTHER 23.9341 uM; ACTIVE	Not recorded	pubchem.aid:2060322 assay Primary qHTS for small molecule compounds that upregulate eGFP-tagged delta133p53alpha (in N-terminal) cells. evidence reference pubchem.aid:2060322 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2060322, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 23.9341 units uM
Primary qHTS for small molecule compounds that upregulate eGFP-tagged delta133p53alpha (in N-terminal) cells.	OTHER 23.9341 uM; ACTIVE	Not recorded	pubchem.aid:2060322 assay Primary qHTS for small molecule compounds that upregulate eGFP-tagged delta133p53alpha (in N-terminal) cells. evidence reference pubchem.aid:2060322 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2060322, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_001119587 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE measurement type OTHER value 23.9341 units uM
Primary qHTS to identify anti-liver cancer compounds using libraries of approved drugs and bioactive compounds	OTHER 15.8489 uM; NOT_DETERMINED	Not recorded	pubchem.aid:2202548 assay Primary qHTS to identify anti-liver cancer compounds using libraries of approved drugs and bioactive compounds evidence reference pubchem.aid:2202548 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202548, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 15.8489 units uM
Primary redox qHTS assay for identification of artifact compounds.	OTHER 29.9349 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1845223 assay Primary redox qHTS assay for identification of artifact compounds. evidence reference pubchem.aid:1845223 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1845223, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 29.9349 units uM
qHTS for Inhibitors of the Aldehyde Dehydrogenase Family (ALDH1A2) Primary screen	OTHER 1.5849 uM; NOT_DETERMINED	Not recorded	pubchem.aid:2202376 assay qHTS for Inhibitors of the Aldehyde Dehydrogenase Family (ALDH1A2) Primary screen evidence reference pubchem.aid:2202376 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202376, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 1.5849 units uM
High Throughput Screen to Identify Inhibitors of Mycobacterium tuberculosis H37Rv	ACTIVE	Not recorded	pubchem.aid:1332 assay High Throughput Screen to Identify Inhibitors of Mycobacterium tuberculosis H37Rv evidence reference pubchem.aid:1332 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1332, deposited by Southern Research Institute; assay type Confirmatory. call ACTIVE activity class ENZYME_INHIBITOR
qHTS for Inhibitors of the Aldehyde Dehydrogenase Family (ALDH1A2) Confirmation screens	ACTIVE	Not recorded	pubchem.aid:2061314 assay qHTS for Inhibitors of the Aldehyde Dehydrogenase Family (ALDH1A2) Confirmation screens evidence reference pubchem.aid:2061314 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2061314, deposited by NCGC; assay type Confirmatory. call ACTIVE activity class ENZYME_INHIBITOR
qHTS for Inhibitors of the Aldehyde Dehydrogenase Family (ALDH1A2) Confirmation screens	ACTIVE	UniProtKB:O60503	pubchem.aid:2061314 assay qHTS for Inhibitors of the Aldehyde Dehydrogenase Family (ALDH1A2) Confirmation screens evidence reference pubchem.aid:2061314 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2061314, deposited by NCGC; assay type Confirmatory. call ACTIVE target enzyme UniProtKB:O60503 activity class ENZYME_INHIBITOR
qHTS for Inhibitors of the Aldehyde Dehydrogenase Family (ALDH3A1) Primary screen	ACTIVE	Not recorded	pubchem.aid:2202377 assay qHTS for Inhibitors of the Aldehyde Dehydrogenase Family (ALDH3A1) Primary screen evidence reference pubchem.aid:2202377 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202377, deposited by NCGC; assay type Confirmatory. call ACTIVE activity class ENZYME_INHIBITOR

Explore across X-Mech

AntibioticMech (chlortetracycline) ↗ MediaIngredientMech (naturalproductmech:mibig-8252d9cf90) ↗ TaxonMech: Kitasatospora aureofaciens (NCBITaxon:1894) ↗ PathwayMech (mibig:BGC0000209) ↗

Provenance

Source concept	Source	Version
BGC0000209	MIBIG	5

This page is generated from data/natural_products/polyketides/chlortetracycline.yaml, which is generated from the committed inventories. Neither is edited by hand.