NaturalProductMech

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daunorubicin

CHEBI:41977 EXACT SEEDED antimicrobial unspecifiedcytotoxic

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

A natural product found in Actinomadura roseola.

Structure

Standard InChIKeySTQGQHZAVUOBTE-VGBVRHCVSA-N
SMILESC[C@H]1[C@H]([C@H](C[C@@H](O1)O[C@H]2C[C@@](CC3=C(C4=C(C(=C23)O)C(=O)C5=C(C4=O)C=CC=C5OC)O)(C(=O)C)O)N)O
Stereochemistryfully defined
Cross-referencespubchem:30323

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Streptomyces sp.
NCBITaxon:1931		 SOURCE_ASSERTION mibig:BGC0000217 PMID:7928998

Occurrences 25

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Streptomyces coeruleorubidus
NCBITaxon:116188		 LOTUS DOI:10.7164/ANTIBIOTICS.33.1158
Streptomyces
NCBITaxon:1883		 LOTUS DOI:10.1021/NP049826V
Streptomyces griseus
NCBITaxon:1911		 LOTUS DOI:10.1002/JOBM.3630240102
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1002/JOBM.201200302
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1007/BF02811416
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1007/S11274-013-1442-X
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1016/J.ABB.2004.03.011
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1016/J.IJBIOMAC.2010.01.019
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1016/J.IJBIOMAC.2013.11.007
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1016/J.JBIOTEC.2017.12.024
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1016/J.MICRES.2009.04.002
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1016/J.MICRES.2018.05.007
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1016/J.PEP.2009.05.012
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1038/NBT0198-69
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1073/PNAS.86.9.3135
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1073/PNAS.88.19.8553
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1074/JBC.M113.536136
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1099/00221287-146-6-1457
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1099/13500872-141-4-1007
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1107/S2053230X15014272
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1111/J.1574-6968.2010.01905.X
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1111/J.1574-6968.2010.01948.X
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1128/JB.174.5.1641-1646.1992
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1128/JB.177.22.6688-6692.1995
Streptomyces peucetius
NCBITaxon:1950		 LOTUS DOI:10.1128/JB.177.5.1208-1215.1995

Biosynthetic gene clusters 1

AccessionHostLocus evidenceGenome
mibig:BGC0000217 Streptomyces sp.
NCBITaxon:1931 		CLUSTER_UNSTATED genbank:L34880.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Biosynthesis curated

Written by a curator with claim-level evidence on each step; not derived from a source. This is what the rest of the record exists to support.

StepEnzymeReactionSubstrate → productEvidence
1 S. peucetius DnrK sequence reference
UniProtKB:Q06528		 ungrounded → CHEBI:41977 https://github.com/mite-standard/mite_data/blob/6eacd913b2036376031072f69cbb33908f132998/mite_data/data/MITE0000492.json

Step 1: MITE0000492-reported carminomycin 4-O-methyltransferase reaction converting carminomycin to daunorubicin (CHEBI:41977) by Streptomyces peucetius DnrK (UniProtKB:Q06528, AAA26712.1 on L13453.1 / AAA99001.1 on L40425.1). Synchronizes biosynthetic_pathway with the existing MITE-backed causal_graph without upgrading producer or locus evidence grades.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 1 producer (0 causal) 25 cited occurrences BIOSYNTHETIC LOCUS 1 gene cluster 1 curated pathway step NATURAL PRODUCT daunorubicin CHEBI:41977 Polyketides TARGETS & BIOACTIVITY 0 molecular targets 25 bioactivities · 2 graphs

Causal graph — bioactivity 4 nodes, 4 edges

Daunorubicin inhibition of recombinant Plasmodium falciparum 3D7 PfUDN ATPase and DNA-unwinding activities.

SubjectPredicateObjectNotesEvidence
P. falciparum PfUDN fragment has activity PfUDN ssDNA-dependent ATPase activity The expressed PfUDN construct spans amino acids 35-727 of the 1441 aa P. falciparum 3D7 Q8I3W6 DNA 3'-5' helicase.
DOI:10.1371/journal.pone.0049385
  • reference
    DOI:10.1371/journal.pone.0049385
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    possess ssDNA-dependent ATPase and DNA helicase activities in vitro
    notes
    Q8I3W6 resolves in UniProtKB to PF3D7_0514100 from P. falciparum isolate 3D7 and annotates a UvrD-like helicase ATP-binding domain over amino acids 35-727.
P. falciparum PfUDN fragment has activity PfUDN DNA unwinding activity The expressed PfUDN construct spans amino acids 35-727 of the 1441 aa P. falciparum 3D7 Q8I3W6 DNA 3'-5' helicase.
DOI:10.1371/journal.pone.0049385
  • reference
    DOI:10.1371/journal.pone.0049385
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    possess ssDNA-dependent ATPase and DNA helicase activities in vitro
    notes
    The authors showed ATP-dependent 3' to 5' DNA unwinding by purified PfUDN.
daunorubicin inhibits PfUDN ssDNA-dependent ATPase activity PubChem AID 1802684 captures the daunorubicin 4.4 uM IC50 against P. falciparum DNA helicase [35-727].
DOI:10.1186/1471-2091-15-9
  • reference
    DOI:10.1186/1471-2091-15-9
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    we screened the effect of various DNA interacting compounds on the ATPase and helicase activity of PfUDN
daunorubicin inhibits PfUDN DNA unwinding activity PubChem AID 1802685 captures the daunorubicin 3.0 uM IC50 against P. falciparum DNA helicase [35-727].
DOI:10.1186/1471-2091-15-9
  • reference
    DOI:10.1186/1471-2091-15-9
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    we screened the effect of various DNA interacting compounds on the ATPase and helicase activity of PfUDN

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 4 nodes, 3 edges

Database-asserted DnrK carminomycin methylation yielding the exact daunorubicin structure. The S. peucetius sequence reference is verified against two genomic DNA deposits; primary experiments were not inspected. This does not assign DnrK to the owner's distinct BGC0000217/L34880.1 source or upgrade producer evidence. Existing PfUDN bioactivity context and the AntibioticMech link are untouched; not full-record sign-off.

SubjectPredicateObjectNotesEvidence
S. peucetius DnrK sequence reference is_database_reported_catalyst_of Database-reported carminomycin 4-O-methylation Protein identity and function are separate claims. The sequence-grounded S. peucetius reference is not asserted to be from the owner's BGC0000217/NCBITaxon:1931 source. No initiator processing, PDB-chain identity, catalytic residue, physiological oligomer, rate or assay condition is claimed.
https://github.com/mite-standard/mite_data/blob/6eacd913b2036376031072f69cbb33908f132998/mite_data/data/MITE0000492.json
https://www.uniprot.org/uniprotkb/Q06528
https://www.ncbi.nlm.nih.gov/protein/AAA26712.1
https://www.ncbi.nlm.nih.gov/protein/AAA99001.1
https://www.ncbi.nlm.nih.gov/nuccore/L13453.1
https://www.ncbi.nlm.nih.gov/nuccore/L40425.1
  • reference
    https://github.com/mite-standard/mite_data/blob/6eacd913b2036376031072f69cbb33908f132998/mite_data/data/MITE0000492.json
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-07
    notes
    MITE0000492 version 1, active, at the pinned commit. It assigns carminomycin 4-O-methylation to DnrK and names daunorubicin as the product. Its heterologous-expression and in-vitro evidence codes are not independently inspected primary experiments.
    snippet
    methylation of the 4-hydroxyl group
  • reference
    https://www.uniprot.org/uniprotkb/Q06528
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-07
    notes
    Reviewed entry 113, sequence 2. FUNCTION assigns methyl transfer from S-adenosyl-L-methionine to carminomycin's 4-O position yielding daunorubicin. Its experimentally coded annotation remains database evidence here: the cited primary paper was inaccessible. No substrate-promiscuity, quaternary-equilibrium or measured kinetic claim is adopted.
    snippet
    carminomycin to form daunorubicin
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AAA26712.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-07
    notes
    Complete conceptual translation and independent coded_by inspected. Source is S. peucetius/taxon 1950 and records ATCC 29050 in the legacy tissue_lib qualifier; this is database source metadata, not authenticated historical stock.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AAA99001.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-07
    notes
    Independent alternate dnrK protein record from L40425.1; complete sequence and coded_by checked. Its 356 residues match AAA26712.1 and current UniProt Q06528.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/L13453.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-07
    notes
    Complete 1071-nt CDS at 204..1274, plus strand, table 11, codon_start 1. Full translation matches its CDS annotation, independent AAA26712.1 and UniProt356aa. This is a genomic sequence deposit for clone pWHM902, not a verified complete expression construct or historical assay-stock sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/L40425.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-07
    notes
    Complete dnrK CDS at 650..1720, plus strand, 1071nt/table11. Coding DNA is identical to the L13453.1 CDS; full translation matches independent AAA99001.1 and UniProt356aa. Nearby putative dnrD/dnrP annotations are not adopted as pathway experiments or as evidence for a physical enzyme complex.
Carminomycin, database-reported substrate is_database_reported_substrate_of Database-reported carminomycin 4-O-methylation MITE and UniProt name carminomycin as the substrate. No external substrate identifier, protonation-state equivalence or additional upstream step is assigned.
https://github.com/mite-standard/mite_data/blob/6eacd913b2036376031072f69cbb33908f132998/mite_data/data/MITE0000492.json
https://www.uniprot.org/uniprotkb/Q06528
  • reference
    https://github.com/mite-standard/mite_data/blob/6eacd913b2036376031072f69cbb33908f132998/mite_data/data/MITE0000492.json
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-07
    notes
    MITE0000492 version 1, active, at the pinned commit. It assigns carminomycin 4-O-methylation to DnrK and names daunorubicin as the product. Its heterologous-expression and in-vitro evidence codes are not independently inspected primary experiments.
  • reference
    https://www.uniprot.org/uniprotkb/Q06528
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-07
    notes
    Reviewed entry 113, sequence 2. FUNCTION assigns methyl transfer from S-adenosyl-L-methionine to carminomycin's 4-O position yielding daunorubicin. Its experimentally coded annotation remains database evidence here: the cited primary paper was inaccessible. No substrate-promiscuity, quaternary-equilibrium or measured kinetic claim is adopted.
Database-reported carminomycin 4-O-methylation has_database_reported_product daunorubicin Pinned MITE product SMILES matches the full owner InChIKey STQGQHZAVUOBTE-VGBVRHCVSA-N and defined stereochemistry using the repository structure helper and RDKit2026.03.5. No additional downstream doxorubicin step or claim of pathway finality is inferred. Producer/BGC grades, occurrences and all existing bioactivity evidence remain unchanged.
https://github.com/mite-standard/mite_data/blob/6eacd913b2036376031072f69cbb33908f132998/mite_data/data/MITE0000492.json
https://www.uniprot.org/uniprotkb/Q06528
  • reference
    https://github.com/mite-standard/mite_data/blob/6eacd913b2036376031072f69cbb33908f132998/mite_data/data/MITE0000492.json
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-07
    notes
    MITE0000492 version 1, active, at the pinned commit. It assigns carminomycin 4-O-methylation to DnrK and names daunorubicin as the product. Its heterologous-expression and in-vitro evidence codes are not independently inspected primary experiments.
  • reference
    https://www.uniprot.org/uniprotkb/Q06528
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-07
    notes
    Reviewed entry 113, sequence 2. FUNCTION assigns methyl transfer from S-adenosyl-L-methionine to carminomycin's 4-O position yielding daunorubicin. Its experimentally coded annotation remains database evidence here: the cited primary paper was inaccessible. No substrate-promiscuity, quaternary-equilibrium or measured kinetic claim is adopted.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Bioactivities 25

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

AssayResultTarget enzymeSource detail
5-hydroxytryptamine receptor 2A (HTR2A) small molecule agonists, cell-based qHTS assayOTHER 21.3138 uM; ACTIVENot recorded
pubchem.aid:1963587
assay
5-hydroxytryptamine receptor 2A (HTR2A) small molecule agonists, cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963587
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963587, deposited by 824; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
21.3138
units
uM
5-hydroxytryptamine receptor 2A (HTR2A) small molecule agonists, cell-based qHTS assayOTHER 21.3138 uM; ACTIVENot recorded
pubchem.aid:1963587
assay
5-hydroxytryptamine receptor 2A (HTR2A) small molecule agonists, cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963587
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963587, deposited by 824; assay type Confirmatory. Source endpoint: Potency. Target accession AAH96839 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
21.3138
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 0 hourOTHER 26.8325 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224869
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 0 hour
evidence
  • reference
    pubchem.aid:1224869
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224869, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
26.8325
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 16 hourOTHER 26.8325 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224888
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 16 hour
evidence
  • reference
    pubchem.aid:1224888
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224888, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
26.8325
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 8 hourOTHER 26.8325 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224884
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 8 hour
evidence
  • reference
    pubchem.aid:1224884
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224884, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
26.8325
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 0 hourOTHER 26.8325 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224882
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 0 hour
evidence
  • reference
    pubchem.aid:1224882
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224882, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
26.8325
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 16 hourOTHER 26.8325 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224876
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 16 hour
evidence
  • reference
    pubchem.aid:1224876
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224876, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
26.8325
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 24 hourOTHER 26.8325 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224878
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 24 hour
evidence
  • reference
    pubchem.aid:1224878
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224878, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
26.8325
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 32 hourOTHER 0.0301 uM; ACTIVENot recorded
pubchem.aid:1224883
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 32 hour
evidence
  • reference
    pubchem.aid:1224883
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224883, deposited by 824; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.0301
units
uM
activity class
CYTOTOXIC
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 40 hourOTHER 0.0239 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224879
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 40 hour
evidence
  • reference
    pubchem.aid:1224879
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224879, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.0239
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 8 hourOTHER 26.8325 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224890
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 8 hour
evidence
  • reference
    pubchem.aid:1224890
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224890, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
26.8325
units
uM
A High-Content qHTS Against Drug Library for the Identification of Small Molecules that Inhibit DYT1 Early Onset Primary Dystonia-Associated Cell PathologyOTHER 1.5849 uM; ACTIVENot recorded
pubchem.aid:1645839
assay
A High-Content qHTS Against Drug Library for the Identification of Small Molecules that Inhibit DYT1 Early Onset Primary Dystonia-Associated Cell Pathology
evidence
  • reference
    pubchem.aid:1645839
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645839, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
1.5849
units
uM
A High-Content qHTS Against Drug Library for the Identification of Small Molecules that Inhibit DYT1 Early Onset Primary Dystonia-Associated Cell PathologyOTHER 1.5849 uM; ACTIVEUniProtKB:O14656
pubchem.aid:1645839
assay
A High-Content qHTS Against Drug Library for the Identification of Small Molecules that Inhibit DYT1 Early Onset Primary Dystonia-Associated Cell Pathology
evidence
  • reference
    pubchem.aid:1645839
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645839, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
1.5849
units
uM
target enzyme
UniProtKB:O14656
A quantitative high throughput screen for small molecules that induce DNA re-replication in SW480 colon adenocarcinoma cells.OTHER 5.3091 uM; INACTIVENot recorded
pubchem.aid:624297
assay
A quantitative high throughput screen for small molecules that induce DNA re-replication in SW480 colon adenocarcinoma cells.
evidence
  • reference
    pubchem.aid:624297
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 624297, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_056979 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
INACTIVE
measurement type
OTHER
value
5.3091
units
uM
Acetylcholinesterase (AChE) small molecule antagonists: colormetric cell-based qHTS assayOTHER 27.5404 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1347395
assay
Acetylcholinesterase (AChE) small molecule antagonists: colormetric cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1347395
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1347395, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession AAA68151 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
27.5404
units
uM
Acetylcholinesterase (AChE) small molecule antagonists: fluorescence cell-based qHTS assayOTHER 38.9018 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1347398
assay
Acetylcholinesterase (AChE) small molecule antagonists: fluorescence cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1347398
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1347398, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession AAA68151 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
38.9018
units
uM
Adrenergic beta2 receptor (ADRB2) small molecule agonists, cell-based qHTS assay: SummaryOTHER 0.35476 uM; NOT_DETERMINEDNot recorded
pubchem.aid:2061107
assay
Adrenergic beta2 receptor (ADRB2) small molecule agonists, cell-based qHTS assay: Summary
evidence
  • reference
    pubchem.aid:2061107
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2061107, deposited by 824; assay type Summary. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.35476
units
uM
Adrenergic beta2 receptor (ADRB2) small molecule agonists, cell-based qHTS assay: SummaryOTHER 0.35476 uM; NOT_DETERMINEDNot recorded
pubchem.aid:2061107
assay
Adrenergic beta2 receptor (ADRB2) small molecule agonists, cell-based qHTS assay: Summary
evidence
  • reference
    pubchem.aid:2061107
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2061107, deposited by 824; assay type Summary. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_000015 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
0.35476
units
uM
Adrenergic beta2 receptor (ADRB2) small molecule antagonists, cell-based qHTS assayOTHER 29.8493 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1963592
assay
Adrenergic beta2 receptor (ADRB2) small molecule antagonists, cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963592
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963592, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
29.8493
units
uM
Adrenergic beta2 receptor (ADRB2) small molecule antagonists, cell-based qHTS assayOTHER 29.8493 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1963592
assay
Adrenergic beta2 receptor (ADRB2) small molecule antagonists, cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963592
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963592, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_000015 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
29.8493
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-191 cell lineOTHER 0.1864 uM; ACTIVENot recorded
pubchem.aid:2202236
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-191 cell line
evidence
  • reference
    pubchem.aid:2202236
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202236, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.1864
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-194 cell lineOTHER 0.0833 uM; NOT_DETERMINEDNot recorded
pubchem.aid:2202237
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-194 cell line
evidence
  • reference
    pubchem.aid:2202237
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202237, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.0833
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: HNT-34 cell lineOTHER 0.1481 uM; NOT_DETERMINEDNot recorded
pubchem.aid:2202234
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: HNT-34 cell line
evidence
  • reference
    pubchem.aid:2202234
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202234, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.1481
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: MV-4-11 cell lineOTHER 0.059 uM; NOT_DETERMINEDNot recorded
pubchem.aid:2202233
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: MV-4-11 cell line
evidence
  • reference
    pubchem.aid:2202233
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202233, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.059
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: OCI-AML3 cell lineOTHER 0.0934 uM; ACTIVENot recorded
pubchem.aid:2202232
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: OCI-AML3 cell line
evidence
  • reference
    pubchem.aid:2202232
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202232, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.0934
units
uM
notes
589 further PubChem assay results are in data/raw/pubchem_bioassay.tsv but not written here.

Elsewhere in the fleet

Explore across X-Mech

AntibioticMech (daunorubicin) ↗ MediaIngredientMech (CHEBI:41977) ↗ TaxonMech: Streptomyces sp. (NCBITaxon:1931) ↗ PathwayMech (mibig:BGC0000217) ↗

Provenance

Source conceptSourceVersion
BGC0000217MIBIG5
CHEBI:41977CHEBI3-star

This page is generated from data/natural_products/polyketides/daunorubicin.yaml, which is generated from the committed inventories. Neither is edited by hand.