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griseofulvin

naturalproductmech:mibig-e59592cca0 MINTED SEEDED cytotoxic

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.
What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKeyDDUHZTYCFQRHIY-OEWHWVHBSA-N
SMILESCOC1=CC(OC)=C(Cl)C2=C1C(=O)C1(O2)[C@H](C)CC(=O)C=C1OC
Stereochemistry undefined stereocentres — the InChIKey does not distinguish this compound from its stereoisomers
Cross-referencespubchem:441140, npatlas:NPA02483

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Penicillium aethiopicum
NCBITaxon:36650		 BGC_CHARACTERIZED mibig:BGC0000070 DOI:10.1021/cb400541z

Occurrences 1

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Penicillium griseum
NCBITaxon:1131486		 LOTUS DOI:10.1042/BJ0330240

Biosynthetic gene clusters 1

AccessionHostLocus evidenceGenome
mibig:BGC0000070 Penicillium aethiopicum
NCBITaxon:36650 		CLUSTER_DEMONSTRATED genbank:GU574478.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Biosynthesis curated

Written by a curator with claim-level evidence on each step; not derived from a source. This is what the rest of the record exists to support.

StepEnzymeReactionSubstrate → productEvidence
1 GsfA non-reducing polyketide synthase
UniProtKB:D7PI15		 RHEA:73915 CHEBI:57288 + CHEBI:57384 → CHEBI:193062 DOI:10.1021/cb400541z
2 GsfB O-methyltransferase
UniProtKB:D7PI16		 RHEA:73923 CHEBI:193062 → CHEBI:193064 DOI:10.1021/cb400541z
3 GsfC O-methyltransferase
UniProtKB:D7PI17		 RHEA:73927 CHEBI:193064 → CHEBI:193065 DOI:10.1021/cb400541z
4 GsfI flavin-dependent halogenase
UniProtKB:D7PI14		 RHEA:73931 CHEBI:193065 → CHEBI:193066 DOI:10.1021/cb400541z
5 GsfF cytochrome P450 monooxygenase
UniProtKB:D7PI20		 RHEA:73935 CHEBI:193066 → CHEBI:193067 DOI:10.1021/cb400541z
6 GsfD O-methyltransferase
UniProtKB:D7PI18		 RHEA:73939 CHEBI:193067 → CHEBI:81999 DOI:10.1021/cb400541z
7 GsfE short-chain dehydrogenase
UniProtKB:D7PI19		 RHEA:73943 CHEBI:81999 → naturalproductmech:mibig-e59592cca0 DOI:10.1021/cb400541z

Step 1: GsfA is proposed to form the heptaketide benzophenone from acetyl-CoA and six malonyl-CoA units; Rhea captures this non-reducing polyketide synthase reaction as RHEA:73915.

Step 2: GsfB methylates the GsfA benzophenone product at 3-OH to yield griseophenone D, the immediate substrate for GsfC; Rhea captures the SAM-dependent methyltransferase reaction as RHEA:73923.

Step 3: GsfC methylates griseophenone D at 9-OH to yield griseophenone C, the immediate substrate for GsfI; Rhea captures the SAM-dependent methyltransferase reaction as RHEA:73927.

Step 4: GsfI chlorinates griseophenone C at C13 to form griseophenone B immediately upstream of GsfF-catalyzed grisan scaffold formation; Rhea captures the reduced-flavin chlorination as RHEA:73931.

Step 5: GsfF performs the late oxidative phenolic coupling that converts griseophenone B into the grisan scaffold of desmethyl-dehydrogriseofulvin during griseofulvin biosynthesis.

Step 6: GsfD is proposed to methylate desmethyl-dehydrogriseofulvin at 5-OH to yield dehydrogriseofulvin; Rhea maps the SAM-dependent GsfD O-methyltransferase to RHEA:73939.

Step 7: GsfE stereospecifically reduces dehydrogriseofulvin to griseofulvin with NADPH; Rhea maps this final reductase reaction to RHEA:73943.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 1 producer (1 causal) 1 cited occurrence BIOSYNTHETIC LOCUS 1 gene cluster 7 curated pathway steps NATURAL PRODUCT griseofulvin naturalproductmech:mibig-e59592cca0 Polyketides TARGETS & BIOACTIVITY 0 molecular targets 25 bioactivities · 7 graphs

Causal graph — biosynthesis 4 nodes, 3 edges

Partial P. aethiopicum griseofulvin biosynthesis graph for proposed GsfA-catalyzed heptaketide benzophenone formation.

SubjectPredicateObjectNotesEvidence
acetyl-CoA(4-) is extended by GsfA non-reducing polyketide synthase GsfA is proposed to prime the griseofulvin heptaketide backbone with acetyl-CoA before extension by malonyl-CoA.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI15
https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    most likely that the true heptaketide product of GsfA is the benzophenone 5a
    notes
    Cacho et al. purified recombinant holo-GsfA from Saccharomyces cerevisiae and observed that GsfA alone turned over malonyl-CoA to norlichexanthone; they reasoned that the true heptaketide product in griseofulvin biosynthesis is the benzophenone 5a that spontaneously dehydrates to norlichexanthone.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24953.1 to GsfA.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI15
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI15 is the 1790 aa P. aethiopicum GsfA non-reducing polyketide synthase, cross-references GU574478 protein ADI24953.1, and annotates RHEA:73915 from PMID:23978092.
  • reference
    https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    The Rhea Swiss-Prot mapping links UniProtKB:D7PI15 to the master GsfA reaction RHEA:73915 and left-to-right reaction RHEA:73916.
malonyl-CoA(5-) is condensed by GsfA non-reducing polyketide synthase GsfA sequentially condenses six malonyl-CoA units while building the benzophenone precursor.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI15
https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    most likely that the true heptaketide product of GsfA is the benzophenone 5a
    notes
    Cacho et al. purified recombinant holo-GsfA from Saccharomyces cerevisiae and observed that GsfA alone turned over malonyl-CoA to norlichexanthone; they reasoned that the true heptaketide product in griseofulvin biosynthesis is the benzophenone 5a that spontaneously dehydrates to norlichexanthone.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24953.1 to GsfA.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI15
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI15 is the 1790 aa P. aethiopicum GsfA non-reducing polyketide synthase, cross-references GU574478 protein ADI24953.1, and annotates RHEA:73915 from PMID:23978092.
  • reference
    https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    The Rhea Swiss-Prot mapping links UniProtKB:D7PI15 to the master GsfA reaction RHEA:73915 and left-to-right reaction RHEA:73916.
GsfA non-reducing polyketide synthase forms 2-(2,4-dihydroxy-6-oxidobenzoyl)-5-hydroxy-3-methylbenzenolate The labile GsfA benzophenone product is inferred to be the immediate upstream substrate for GsfB; when it is not captured by GsfB, it can spontaneously dehydrate to the off-pathway shunt product norlichexanthone.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI15
https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    most likely that the true heptaketide product of GsfA is the benzophenone 5a
    notes
    Cacho et al. purified recombinant holo-GsfA from Saccharomyces cerevisiae and observed that GsfA alone turned over malonyl-CoA to norlichexanthone; they reasoned that the true heptaketide product in griseofulvin biosynthesis is the benzophenone 5a that spontaneously dehydrates to norlichexanthone.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24953.1 to GsfA.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI15
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI15 is the 1790 aa P. aethiopicum GsfA non-reducing polyketide synthase, cross-references GU574478 protein ADI24953.1, and annotates RHEA:73915 from PMID:23978092.
  • reference
    https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    The Rhea Swiss-Prot mapping links UniProtKB:D7PI15 to the master GsfA reaction RHEA:73915 and left-to-right reaction RHEA:73916.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 3 nodes, 2 edges

Partial P. aethiopicum griseofulvin biosynthesis graph for GsfB-catalyzed 3-O-methylation.

SubjectPredicateObjectNotesEvidence
2-(2,4-dihydroxy-6-oxidobenzoyl)-5-hydroxy-3-methylbenzenolate is methylated by GsfB O-methyltransferase GsfB is the 3-OH O-methyltransferase that installs the methyl group yielding griseophenone D.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI16
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfB is the 3-OH MT
    notes
    Cacho et al. deleted gsfB and observed griseophenone-family intermediates that lacked 3-OH methylation; a coupled GsfA/GsfB in vitro assay further supported GsfB 3-OH O-methyltransferase activity and produced griseophenone D.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24954.1 to GsfB.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI16
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI16 is the 419 aa P. aethiopicum GsfB O-methyltransferase, cross-references GU574478 protein ADI24954.1, and annotates RHEA:73923 from PMID:23978092.
GsfB O-methyltransferase forms griseophenone D The SAM-dependent GsfB reaction converts the GsfA benzophenone product into griseophenone D, placing GsfB immediately upstream of GsfC-catalyzed methylation.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI16
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfB is the 3-OH MT
    notes
    Cacho et al. deleted gsfB and observed griseophenone-family intermediates that lacked 3-OH methylation; a coupled GsfA/GsfB in vitro assay further supported GsfB 3-OH O-methyltransferase activity and produced griseophenone D.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24954.1 to GsfB.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI16
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI16 is the 419 aa P. aethiopicum GsfB O-methyltransferase, cross-references GU574478 protein ADI24954.1, and annotates RHEA:73923 from PMID:23978092.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 3 nodes, 2 edges

Partial P. aethiopicum griseofulvin biosynthesis graph for GsfC-catalyzed griseophenone D 9-O-methylation.

SubjectPredicateObjectNotesEvidence
griseophenone D is methylated by GsfC O-methyltransferase GsfC is the 9-OH O-methyltransferase that installs the methyl group yielding griseophenone C.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI17
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfC is the 9-OH MT
    notes
    Cacho et al. deleted gsfC and observed accumulation of 3-O-methylated, 9-OH-unmethylated intermediates including griseophenone D; coupled GsfA/GsfC in vitro assays further supported GsfC 9-OH O-methyltransferase activity.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24955.1 to GsfC.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI17
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI17 is the 396 aa P. aethiopicum GsfC O-methyltransferase, cross-references GU574478 protein ADI24955.1, and annotates RHEA:73927 from PMID:23978092.
GsfC O-methyltransferase forms griseophenone C The SAM-dependent GsfC reaction converts griseophenone D into griseophenone C, placing GsfC immediately upstream of GsfI-catalyzed halogenation.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI17
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfC is the 9-OH MT
    notes
    Cacho et al. deleted gsfC and observed accumulation of 3-O-methylated, 9-OH-unmethylated intermediates including griseophenone D; coupled GsfA/GsfC in vitro assays further supported GsfC 9-OH O-methyltransferase activity.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24955.1 to GsfC.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI17
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI17 is the 396 aa P. aethiopicum GsfC O-methyltransferase, cross-references GU574478 protein ADI24955.1, and annotates RHEA:73927 from PMID:23978092.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 3 nodes, 2 edges

Partial P. aethiopicum griseofulvin biosynthesis graph for GsfI-catalyzed griseophenone C chlorination.

SubjectPredicateObjectNotesEvidence
griseophenone C is chlorinated by GsfI flavin-dependent halogenase GsfI catalyzes chlorination of griseophenone C to produce griseophenone B; Cacho et al. used SsuE as the NADPH-dependent flavin reductase in the in vitro reconstitution.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI14
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfI led to the production of chlorinated phenone 11
    notes
    Cacho et al. cloned, expressed, and purified GsfI and reconstituted chlorination of griseophenone C, compound 10, to griseophenone B, compound 11, in vitro with SsuE to regenerate the reduced flavin cofactor.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24948.1 to GsfI.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI14
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI14 is the 533 aa P. aethiopicum GsfI flavin-dependent halogenase, cross-references GU574478 protein ADI24948.1, and annotates RHEA:73931 from PMID:23978092.
GsfI flavin-dependent halogenase forms griseophenone B The chlorinated product is compound 11 in the primary paper and is curated as griseophenone B in ChEBI and Rhea.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI14
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfI led to the production of chlorinated phenone 11
    notes
    Cacho et al. cloned, expressed, and purified GsfI and reconstituted chlorination of griseophenone C, compound 10, to griseophenone B, compound 11, in vitro with SsuE to regenerate the reduced flavin cofactor.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24948.1 to GsfI.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI14
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI14 is the 533 aa P. aethiopicum GsfI flavin-dependent halogenase, cross-references GU574478 protein ADI24948.1, and annotates RHEA:73931 from PMID:23978092.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 3 nodes, 2 edges

Partial P. aethiopicum griseofulvin biosynthesis graph for GsfF-catalyzed grisan scaffold formation.

SubjectPredicateObjectNotesEvidence
griseophenone B is oxidatively coupled by GsfF cytochrome P450 monooxygenase GsfF catalyzes oxidative coupling of the orcinol and phloroglucinol rings in griseophenone B.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI20
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    90% conversion of 11 to 14
    notes
    Cacho et al. deleted gsfF in Penicillium aethiopicum, observed griseophenone B accumulation, expressed GsfF with Aspergillus terreus cytochrome P450 reductase in Saccharomyces cerevisiae, and confirmed that GsfF converts griseophenone B, compound 11, to the grisan product desmethyl-dehydrogriseofulvin, compound 14.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24958.2 to GsfF.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI20
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI20 is the 504 aa P. aethiopicum GsfF cytochrome P450 monooxygenase, cross-references GU574478 protein ADI24958.2, and annotates RHEA:73935 from PMID:23978092.
GsfF cytochrome P450 monooxygenase forms desmethyl-dehydrogriseofulvin The spirocyclic product is compound 14 in the primary paper and is curated as desmethyl-dehydrogriseofulvin in ChEBI and Rhea.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI20
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    90% conversion of 11 to 14
    notes
    Cacho et al. deleted gsfF in Penicillium aethiopicum, observed griseophenone B accumulation, expressed GsfF with Aspergillus terreus cytochrome P450 reductase in Saccharomyces cerevisiae, and confirmed that GsfF converts griseophenone B, compound 11, to the grisan product desmethyl-dehydrogriseofulvin, compound 14.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24958.2 to GsfF.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI20
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI20 is the 504 aa P. aethiopicum GsfF cytochrome P450 monooxygenase, cross-references GU574478 protein ADI24958.2, and annotates RHEA:73935 from PMID:23978092.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 3 nodes, 2 edges

Partial P. aethiopicum griseofulvin biosynthesis graph for proposed GsfD-catalyzed 5-O-methylation.

SubjectPredicateObjectNotesEvidence
desmethyl-dehydrogriseofulvin is methylated by GsfD O-methyltransferase GsfD is proposed to methylate the 5-OH of desmethyl-dehydrogriseofulvin.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI18
https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfD regioselectively targets 5-OH
    notes
    Cacho et al. deleted gsfD and observed late-stage griseofulvin intermediates lacking 5-O-methylation; they proposed that GsfD methylates desmethyl-dehydrogriseofulvin, compound 14, to produce dehydrogriseofulvin, compound 18.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24956.1 to GsfD.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI18
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI18 is the 378 aa P. aethiopicum GsfD O-methyltransferase and cross-references GU574478 protein ADI24956.1.
  • reference
    https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    The Rhea Swiss-Prot mapping links UniProtKB:D7PI18 to the master GsfD reaction RHEA:73939 and left-to-right reaction RHEA:73940.
GsfD O-methyltransferase forms dehydrogriseofulvin The proposed SAM-dependent GsfD reaction converts desmethyl-dehydrogriseofulvin to dehydrogriseofulvin before GsfE-catalyzed reduction.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI18
https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfD regioselectively targets 5-OH
    notes
    Cacho et al. deleted gsfD and observed late-stage griseofulvin intermediates lacking 5-O-methylation; they proposed that GsfD methylates desmethyl-dehydrogriseofulvin, compound 14, to produce dehydrogriseofulvin, compound 18.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24956.1 to GsfD.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI18
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI18 is the 378 aa P. aethiopicum GsfD O-methyltransferase and cross-references GU574478 protein ADI24956.1.
  • reference
    https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    The Rhea Swiss-Prot mapping links UniProtKB:D7PI18 to the master GsfD reaction RHEA:73939 and left-to-right reaction RHEA:73940.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 3 nodes, 2 edges

Partial P. aethiopicum griseofulvin biosynthesis graph for GsfE-catalyzed final reduction.

SubjectPredicateObjectNotesEvidence
dehydrogriseofulvin is reduced by GsfE short-chain dehydrogenase GsfE reduces dehydrogriseofulvin with NADPH in the final step of griseofulvin biosynthesis.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI19
https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfE fully converted 18 or 19 to 1 or 2
    notes
    Cacho et al. deleted gsfE and observed dehydrogriseofulvin and dechloro-dehydrogriseofulvin accumulation, then purified His-tagged GsfE and showed NADPH-dependent in vitro conversion of dehydrogriseofulvin, compound 18, to griseofulvin, compound 1.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24957.1 to GsfE.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI19
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI19 is the 377 aa P. aethiopicum GsfE short-chain dehydrogenase and cross-references GU574478 protein ADI24957.1.
  • reference
    https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    The Rhea Swiss-Prot mapping links UniProtKB:D7PI19 to the master GsfE reaction RHEA:73943 and left-to-right reaction RHEA:73944.
GsfE short-chain dehydrogenase forms griseofulvin His-tagged GsfE converted dehydrogriseofulvin, compound 18, to griseofulvin, compound 1, in vitro.
DOI:10.1021/cb400541z
https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
https://www.uniprot.org/uniprotkb/D7PI19
https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
  • reference
    DOI:10.1021/cb400541z
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    GsfE fully converted 18 or 19 to 1 or 2
    notes
    Cacho et al. deleted gsfE and observed dehydrogriseofulvin and dechloro-dehydrogriseofulvin accumulation, then purified His-tagged GsfE and showed NADPH-dependent in vitro conversion of dehydrogriseofulvin, compound 18, to griseofulvin, compound 1.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000070.5/BGC0000070.gbk
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    MIBiG BGC0000070.5 maps the P. aethiopicum GU574478.1 griseofulvin biosynthetic gene cluster CDS ADI24957.1 to GsfE.
  • reference
    https://www.uniprot.org/uniprotkb/D7PI19
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    Reviewed UniProtKB:D7PI19 is the 377 aa P. aethiopicum GsfE short-chain dehydrogenase and cross-references GU574478 protein ADI24957.1.
  • reference
    https://ftp.expasy.org/databases/rhea/tsv/rhea2uniprot_sprot.tsv
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-30
    notes
    The Rhea Swiss-Prot mapping links UniProtKB:D7PI19 to the master GsfE reaction RHEA:73943 and left-to-right reaction RHEA:73944.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Bioactivities 25

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

AssayResultTarget enzymeSource detail
5-hydroxytryptamine receptor 2A (HTR2A) small molecule antagonists, cell-based qHTS assayOTHER 23.4556 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1963586
assay
5-hydroxytryptamine receptor 2A (HTR2A) small molecule antagonists, cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963586
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963586, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
23.4556
units
uM
5-hydroxytryptamine receptor 2A (HTR2A) small molecule antagonists, cell-based qHTS assayOTHER 23.4556 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1963586
assay
5-hydroxytryptamine receptor 2A (HTR2A) small molecule antagonists, cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963586
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963586, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession AAH96839 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
23.4556
units
uM
5-hydroxytryptamine receptor 2A (HTR2A) small molecule antagonists, cell-based qHTS assayOTHER 23.0537 uM; INACTIVENot recorded
pubchem.aid:1963586
assay
5-hydroxytryptamine receptor 2A (HTR2A) small molecule antagonists, cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963586
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963586, deposited by 824; assay type Confirmatory. Source endpoint: Potency.
call
INACTIVE
measurement type
OTHER
value
23.0537
units
uM
5-hydroxytryptamine receptor 2A (HTR2A) small molecule antagonists, cell-based qHTS assayOTHER 23.0537 uM; INACTIVENot recorded
pubchem.aid:1963586
assay
5-hydroxytryptamine receptor 2A (HTR2A) small molecule antagonists, cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963586
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963586, deposited by 824; assay type Confirmatory. Source endpoint: Potency. Target accession AAH96839 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
INACTIVE
measurement type
OTHER
value
23.0537
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 0 hourOTHER 30.6312 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224869
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 0 hour
evidence
  • reference
    pubchem.aid:1224869
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224869, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
30.6312
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 16 hourOTHER 30.6312 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224888
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 16 hour
evidence
  • reference
    pubchem.aid:1224888
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224888, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
30.6312
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 24 hourOTHER 34.3687 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224875
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 24 hour
evidence
  • reference
    pubchem.aid:1224875
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224875, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
34.3687
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 32 hourOTHER 38.5624 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224881
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 32 hour
evidence
  • reference
    pubchem.aid:1224881
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224881, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
38.5624
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 40 hourOTHER 38.5624 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224871
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 40 hour
evidence
  • reference
    pubchem.aid:1224871
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224871, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
38.5624
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 8 hourOTHER 24.3312 uM; ACTIVENot recorded
pubchem.aid:1224884
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HEK293 cells - 8 hour
evidence
  • reference
    pubchem.aid:1224884
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224884, deposited by 824; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
24.3312
units
uM
activity class
CYTOTOXIC
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 16 hourOTHER 24.5412 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224876
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 16 hour
evidence
  • reference
    pubchem.aid:1224876
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224876, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
24.5412
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 24 hourOTHER 38.5624 uM; ACTIVENot recorded
pubchem.aid:1224878
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 24 hour
evidence
  • reference
    pubchem.aid:1224878
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224878, deposited by 824; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
38.5624
units
uM
activity class
CYTOTOXIC
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 24 hourOTHER 21.8724 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224878
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 24 hour
evidence
  • reference
    pubchem.aid:1224878
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224878, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
21.8724
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 32 hourOTHER 34.3687 uM; ACTIVENot recorded
pubchem.aid:1224883
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 32 hour
evidence
  • reference
    pubchem.aid:1224883
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224883, deposited by 824; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
34.3687
units
uM
activity class
CYTOTOXIC
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 32 hourOTHER 21.8724 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224883
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 32 hour
evidence
  • reference
    pubchem.aid:1224883
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224883, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
21.8724
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 32 hourOTHER 26.8325 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224883
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 32 hour
evidence
  • reference
    pubchem.aid:1224883
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224883, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
26.8325
units
uM
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 40 hourOTHER 24.3312 uM; ACTIVENot recorded
pubchem.aid:1224879
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 40 hour
evidence
  • reference
    pubchem.aid:1224879
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224879, deposited by 824; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
24.3312
units
uM
activity class
CYTOTOXIC
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 40 hourOTHER 21.8724 uM; ACTIVENot recorded
pubchem.aid:1224879
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 40 hour
evidence
  • reference
    pubchem.aid:1224879
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224879, deposited by 824; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
21.8724
units
uM
activity class
CYTOTOXIC
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 40 hourOTHER 26.8325 uM; ACTIVENot recorded
pubchem.aid:1224879
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 40 hour
evidence
  • reference
    pubchem.aid:1224879
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224879, deposited by 824; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
26.8325
units
uM
activity class
CYTOTOXIC
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 8 hourOTHER 48.9662 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1224890
assay
A CellTox Green Cytotoxicity Assay to monitor cytotoxicity in HepG2 cells - 8 hour
evidence
  • reference
    pubchem.aid:1224890
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1224890, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
48.9662
units
uM
A quantitative high throughput screen for small molecules that induce DNA re-replication in MCF 10a normal breast cells.OTHER 21.136 uM; NOT_DETERMINEDNot recorded
pubchem.aid:624296
assay
A quantitative high throughput screen for small molecules that induce DNA re-replication in MCF 10a normal breast cells.
evidence
  • reference
    pubchem.aid:624296
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 624296, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_056979 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
21.136
units
uM
A quantitative high throughput screen for small molecules that induce DNA re-replication in MCF 10a normal breast cells.OTHER 6.5131 uM; NOT_DETERMINEDNot recorded
pubchem.aid:624296
assay
A quantitative high throughput screen for small molecules that induce DNA re-replication in MCF 10a normal breast cells.
evidence
  • reference
    pubchem.aid:624296
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 624296, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_056979 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
6.5131
units
uM
A quantitative high throughput screen for small molecules that induce DNA re-replication in SW480 colon adenocarcinoma cells.OTHER 33.4983 uM; NOT_DETERMINEDNot recorded
pubchem.aid:624297
assay
A quantitative high throughput screen for small molecules that induce DNA re-replication in SW480 colon adenocarcinoma cells.
evidence
  • reference
    pubchem.aid:624297
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 624297, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_056979 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
33.4983
units
uM
A quantitative high throughput screen for small molecules that induce DNA re-replication in SW480 colon adenocarcinoma cells.OTHER 22.3872 uM; NOT_DETERMINEDNot recorded
pubchem.aid:624297
assay
A quantitative high throughput screen for small molecules that induce DNA re-replication in SW480 colon adenocarcinoma cells.
evidence
  • reference
    pubchem.aid:624297
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 624297, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_056979 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
22.3872
units
uM
Caspase-3/7 induction in CHO-K1 cells by small molecules, qHTS cell viability counter screenOTHER 42.8813 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1346979
assay
Caspase-3/7 induction in CHO-K1 cells by small molecules, qHTS cell viability counter screen
evidence
  • reference
    pubchem.aid:1346979
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1346979, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
42.8813
units
uM
notes
328 further PubChem assay results are in data/raw/pubchem_bioassay.tsv but not written here.

Explore across X-Mech

AntibioticMech (griseofulvin) ↗ MediaIngredientMech (naturalproductmech:mibig-e59592cca0) ↗ TaxonMech: Penicillium aethiopicum (NCBITaxon:36650) ↗ PathwayMech (mibig:BGC0000070) ↗

Provenance

Source conceptSourceVersion
BGC0000070MIBIG5

This page is generated from data/natural_products/polyketides/griseofulvin.yaml, which is generated from the committed inventories. Neither is edited by hand.