meilingmycin

naturalproductmech:mibig-13e3c54c7c MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	QSHJCXWGTCXGAX-ZCVXJSSDSA-N
SMILES	C[C@H]1CC[C@]2(C[C@@H]3C[C@H](O2)C/C=C(\C[C@H](/C=C\CC4COC5[C@@H]4[C@@H](C=C([C@H]5O)COC(=O)C=C(C)C)C(=O)O3)C)/C)OC1C
Stereochemistry	undefined stereocentres — the InChIKey does not distinguish this compound from its stereoisomers
Cross-references	pubchem:101260926

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Streptomyces nanchangensis NCBITaxon:204925	SOURCE_ASSERTION	mibig:BGC0000093	DOI:10.1128/aem.02262-09

Occurrences 1

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

Taxon	Source	Reference
Streptomyces nanchangensis NCBITaxon:204925	LOTUS	DOI:10.1007/S00203-003-0564-1

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0000093	Streptomyces nanchangensis NCBITaxon:204925	CLUSTER_UNSTATED	genbank:FJ952082.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Causal graph — biosynthesis 4 nodes, 3 edges

Partial Streptomyces nanchangensis meilingmycin biosynthesis graph for MeiD-catalyzed SAM-dependent C5-O-methylation of a milbemycin-like meilingmycin intermediate.

Subject	Predicate	Object	Notes	Evidence
C5-hydroxy meilingmycin intermediate	is C5-O-methylated by	MeiD C5-O-methyltransferase	MITE represents MeiD methylation on the meilingmycin/milbemycin scaffold. The exact substrate and product are left as local intermediates because MITE supplies reaction structures but no stable names for them.	https://mite.bioinformatics.nl/repository/MITE0000009 DOI:10.1128/AEM.02262-09 https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk https://www.uniprot.org/uniprotkb/D6C4C6 https://www.ncbi.nlm.nih.gov/protein/ADC45587.1 reference https://mite.bioinformatics.nl/repository/MITE0000009 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Methylation notes MITE0000009 is active, lists a knock-out study evidence code, assigns a methylation reaction on the meilingmycin/milbemycin scaffold to MeiD, lists SAM as an organic cofactor, links the reaction row to DOI:10.1128/AEM.02262-09, and links MeiD to UniProtKB:D6C4C6, GenPept ADC45587.1, and MIBiG BGC0000093. reference DOI:10.1128/AEM.02262-09 evidence type PRIMARY_EXPERIMENT notes He et al. localized meiD downstream of the PKS genes encoding the loading and first two elongation modules, described MeiD as a C5-O-methyltransferase, and showed by gene deletion that meiD is required for production of C5-methylated meilingmycins D and E. reference https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes MIBiG BGC0000093.5 places meiD/ADC45587.1 inside the active Streptomyces nanchangensis meilingmycin biosynthetic gene cluster and links the CDS to MITE0000009 at 100% identity. reference https://www.uniprot.org/uniprotkb/D6C4C6 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:D6C4C6 resolves as a 286 aa Streptomyces nanchangensis meiD C5-O-methyltransferase TrEMBL entry, cross-references EMBL FJ952082 protein ADC45587.1, and carries SAM-dependent methyltransferase domain cross-references. reference https://www.ncbi.nlm.nih.gov/protein/ADC45587.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes NCBI Protein ADC45587.1 is the Streptomyces nanchangensis MeiD C5-O-methyltransferase encoded by meiD at FJ952082.1:167087..167947.
S-adenosyl-L-methionine	supplies a methyl group to	MeiD C5-O-methyltransferase	MITE lists SAM as MeiD's organic cofactor for this O-methylation reaction.	https://mite.bioinformatics.nl/repository/MITE0000009 https://www.uniprot.org/uniprotkb/D6C4C6 reference https://mite.bioinformatics.nl/repository/MITE0000009 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Methylation notes MITE0000009 is active, lists a knock-out study evidence code, assigns a methylation reaction on the meilingmycin/milbemycin scaffold to MeiD, lists SAM as an organic cofactor, links the reaction row to DOI:10.1128/AEM.02262-09, and links MeiD to UniProtKB:D6C4C6, GenPept ADC45587.1, and MIBiG BGC0000093. reference https://www.uniprot.org/uniprotkb/D6C4C6 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:D6C4C6 resolves as a 286 aa Streptomyces nanchangensis meiD C5-O-methyltransferase TrEMBL entry, cross-references EMBL FJ952082 protein ADC45587.1, and carries SAM-dependent methyltransferase domain cross-references.
MeiD C5-O-methyltransferase	forms	C5-O-methyl meilingmycin intermediate	MeiD is represented as forming the C5-O-methylated product of MITE's meilingmycin/milbemycin scaffold methylation reaction.	https://mite.bioinformatics.nl/repository/MITE0000009 DOI:10.1128/AEM.02262-09 https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk https://www.uniprot.org/uniprotkb/D6C4C6 https://www.ncbi.nlm.nih.gov/protein/ADC45587.1 reference https://mite.bioinformatics.nl/repository/MITE0000009 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Methylation notes MITE0000009 is active, lists a knock-out study evidence code, assigns a methylation reaction on the meilingmycin/milbemycin scaffold to MeiD, lists SAM as an organic cofactor, links the reaction row to DOI:10.1128/AEM.02262-09, and links MeiD to UniProtKB:D6C4C6, GenPept ADC45587.1, and MIBiG BGC0000093. reference DOI:10.1128/AEM.02262-09 evidence type PRIMARY_EXPERIMENT notes He et al. localized meiD downstream of the PKS genes encoding the loading and first two elongation modules, described MeiD as a C5-O-methyltransferase, and showed by gene deletion that meiD is required for production of C5-methylated meilingmycins D and E. reference https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes MIBiG BGC0000093.5 places meiD/ADC45587.1 inside the active Streptomyces nanchangensis meilingmycin biosynthetic gene cluster and links the CDS to MITE0000009 at 100% identity. reference https://www.uniprot.org/uniprotkb/D6C4C6 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:D6C4C6 resolves as a 286 aa Streptomyces nanchangensis meiD C5-O-methyltransferase TrEMBL entry, cross-references EMBL FJ952082 protein ADC45587.1, and carries SAM-dependent methyltransferase domain cross-references. reference https://www.ncbi.nlm.nih.gov/protein/ADC45587.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes NCBI Protein ADC45587.1 is the Streptomyces nanchangensis MeiD C5-O-methyltransferase encoded by meiD at FJ952082.1:167087..167947.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 7 nodes, 7 edges

Partial Streptomyces nanchangensis meilingmycin biosynthesis graph for MeiE-catalyzed heme-dependent hydroxylation and heterocyclization of milbemycin-like meilingmycin intermediates.

Subject	Predicate	Object	Notes	Evidence
C5-O-methyl meilingmycin intermediate	is hydroxylated by	MeiE cytochrome P450	MITE reaction 2 represents MeiE hydroxylation of the C5-O-methylated meilingmycin/milbemycin scaffold. The exact substrate and product are left as local intermediates because MITE supplies reaction structures but no stable names for them.	https://mite.bioinformatics.nl/repository/MITE0000010 DOI:10.1128/AEM.02262-09 https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk https://www.uniprot.org/uniprotkb/Q6YL50 https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 reference https://mite.bioinformatics.nl/repository/MITE0000010 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Heterocyclization, Hydroxylation notes MITE0000010 is active, lists three knock-out-supported MeiE reaction rows on meilingmycin/milbemycin scaffolds, assigns two rows to hydroxylation and one row to heterocyclization, lists heme and iron as cofactors, links each reaction row to DOI:10.1128/AEM.02262-09, and links MeiE to UniProtKB:Q6YL50, GenPept AAM97314.1, and MIBiG BGC0000093. reference DOI:10.1128/AEM.02262-09 evidence type PRIMARY_EXPERIMENT notes He et al. identified meiE as an aveE homolog in the meilingmycin gene cluster, annotated MeiE as a cytochrome P450 hydroxylase, and proposed that MeiE oxidizes the TE-released, cyclized polyketide to generate a C8-C6 furan ring, a C8 hydroxyl group, or C8 and C6 hydroxyl groups. reference https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes MIBiG BGC0000093.5 places meiE/AAM97314.1 inside the active Streptomyces nanchangensis meilingmycin biosynthetic gene cluster and links the CDS to MITE0000010 at 100% identity. reference https://www.uniprot.org/uniprotkb/Q6YL50 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:Q6YL50 resolves as a 459 aa Streptomyces nanchangensis meiE cytochrome P450 hydroxylase TrEMBL entry, cross-references EMBL FJ952082 protein AAM97314.1, annotates heme as a cofactor, and carries heme-binding and monooxygenase cross-references. reference https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes NCBI Protein AAM97314.1 is the Streptomyces nanchangensis MeiE cytochrome P450 hydroxylase encoded by meiE at complement(FJ952082.1:58480..59859).
MeiE cytochrome P450	forms	hydroxymethylated C5-O-methyl meilingmycin intermediate	This product corresponds to MITE reaction 2's hydroxylated minor-component structure.	https://mite.bioinformatics.nl/repository/MITE0000010 DOI:10.1128/AEM.02262-09 https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk https://www.uniprot.org/uniprotkb/Q6YL50 https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 reference https://mite.bioinformatics.nl/repository/MITE0000010 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Heterocyclization, Hydroxylation notes MITE0000010 is active, lists three knock-out-supported MeiE reaction rows on meilingmycin/milbemycin scaffolds, assigns two rows to hydroxylation and one row to heterocyclization, lists heme and iron as cofactors, links each reaction row to DOI:10.1128/AEM.02262-09, and links MeiE to UniProtKB:Q6YL50, GenPept AAM97314.1, and MIBiG BGC0000093. reference DOI:10.1128/AEM.02262-09 evidence type PRIMARY_EXPERIMENT notes He et al. identified meiE as an aveE homolog in the meilingmycin gene cluster, annotated MeiE as a cytochrome P450 hydroxylase, and proposed that MeiE oxidizes the TE-released, cyclized polyketide to generate a C8-C6 furan ring, a C8 hydroxyl group, or C8 and C6 hydroxyl groups. reference https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes MIBiG BGC0000093.5 places meiE/AAM97314.1 inside the active Streptomyces nanchangensis meilingmycin biosynthetic gene cluster and links the CDS to MITE0000010 at 100% identity. reference https://www.uniprot.org/uniprotkb/Q6YL50 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:Q6YL50 resolves as a 459 aa Streptomyces nanchangensis meiE cytochrome P450 hydroxylase TrEMBL entry, cross-references EMBL FJ952082 protein AAM97314.1, annotates heme as a cofactor, and carries heme-binding and monooxygenase cross-references. reference https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes NCBI Protein AAM97314.1 is the Streptomyces nanchangensis MeiE cytochrome P450 hydroxylase encoded by meiE at complement(FJ952082.1:58480..59859).
hydroxymethylated C5-O-methyl meilingmycin intermediate	is hydroxylated by	MeiE cytochrome P450	MITE reaction 1 represents a second MeiE hydroxylation of a C5-O-methylated meilingmycin/milbemycin minor component.	https://mite.bioinformatics.nl/repository/MITE0000010 DOI:10.1128/AEM.02262-09 https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk https://www.uniprot.org/uniprotkb/Q6YL50 https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 reference https://mite.bioinformatics.nl/repository/MITE0000010 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Heterocyclization, Hydroxylation notes MITE0000010 is active, lists three knock-out-supported MeiE reaction rows on meilingmycin/milbemycin scaffolds, assigns two rows to hydroxylation and one row to heterocyclization, lists heme and iron as cofactors, links each reaction row to DOI:10.1128/AEM.02262-09, and links MeiE to UniProtKB:Q6YL50, GenPept AAM97314.1, and MIBiG BGC0000093. reference DOI:10.1128/AEM.02262-09 evidence type PRIMARY_EXPERIMENT notes He et al. identified meiE as an aveE homolog in the meilingmycin gene cluster, annotated MeiE as a cytochrome P450 hydroxylase, and proposed that MeiE oxidizes the TE-released, cyclized polyketide to generate a C8-C6 furan ring, a C8 hydroxyl group, or C8 and C6 hydroxyl groups. reference https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes MIBiG BGC0000093.5 places meiE/AAM97314.1 inside the active Streptomyces nanchangensis meilingmycin biosynthetic gene cluster and links the CDS to MITE0000010 at 100% identity. reference https://www.uniprot.org/uniprotkb/Q6YL50 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:Q6YL50 resolves as a 459 aa Streptomyces nanchangensis meiE cytochrome P450 hydroxylase TrEMBL entry, cross-references EMBL FJ952082 protein AAM97314.1, annotates heme as a cofactor, and carries heme-binding and monooxygenase cross-references. reference https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes NCBI Protein AAM97314.1 is the Streptomyces nanchangensis MeiE cytochrome P450 hydroxylase encoded by meiE at complement(FJ952082.1:58480..59859).
MeiE cytochrome P450	forms	oxidized minor meilingmycin component	This product corresponds to MITE reaction 1's further oxidized minor-component structure.	https://mite.bioinformatics.nl/repository/MITE0000010 DOI:10.1128/AEM.02262-09 https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk https://www.uniprot.org/uniprotkb/Q6YL50 https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 reference https://mite.bioinformatics.nl/repository/MITE0000010 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Heterocyclization, Hydroxylation notes MITE0000010 is active, lists three knock-out-supported MeiE reaction rows on meilingmycin/milbemycin scaffolds, assigns two rows to hydroxylation and one row to heterocyclization, lists heme and iron as cofactors, links each reaction row to DOI:10.1128/AEM.02262-09, and links MeiE to UniProtKB:Q6YL50, GenPept AAM97314.1, and MIBiG BGC0000093. reference DOI:10.1128/AEM.02262-09 evidence type PRIMARY_EXPERIMENT notes He et al. identified meiE as an aveE homolog in the meilingmycin gene cluster, annotated MeiE as a cytochrome P450 hydroxylase, and proposed that MeiE oxidizes the TE-released, cyclized polyketide to generate a C8-C6 furan ring, a C8 hydroxyl group, or C8 and C6 hydroxyl groups. reference https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes MIBiG BGC0000093.5 places meiE/AAM97314.1 inside the active Streptomyces nanchangensis meilingmycin biosynthetic gene cluster and links the CDS to MITE0000010 at 100% identity. reference https://www.uniprot.org/uniprotkb/Q6YL50 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:Q6YL50 resolves as a 459 aa Streptomyces nanchangensis meiE cytochrome P450 hydroxylase TrEMBL entry, cross-references EMBL FJ952082 protein AAM97314.1, annotates heme as a cofactor, and carries heme-binding and monooxygenase cross-references. reference https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes NCBI Protein AAM97314.1 is the Streptomyces nanchangensis MeiE cytochrome P450 hydroxylase encoded by meiE at complement(FJ952082.1:58480..59859).
C5-hydroxy meilingmycin intermediate	is heterocyclized by	MeiE cytochrome P450	MITE reaction 3 represents MeiE heterocyclization on an unmethylated meilingmycin/milbemycin intermediate.	https://mite.bioinformatics.nl/repository/MITE0000010 DOI:10.1128/AEM.02262-09 https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk https://www.uniprot.org/uniprotkb/Q6YL50 https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 reference https://mite.bioinformatics.nl/repository/MITE0000010 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Heterocyclization, Hydroxylation notes MITE0000010 is active, lists three knock-out-supported MeiE reaction rows on meilingmycin/milbemycin scaffolds, assigns two rows to hydroxylation and one row to heterocyclization, lists heme and iron as cofactors, links each reaction row to DOI:10.1128/AEM.02262-09, and links MeiE to UniProtKB:Q6YL50, GenPept AAM97314.1, and MIBiG BGC0000093. reference DOI:10.1128/AEM.02262-09 evidence type PRIMARY_EXPERIMENT notes He et al. identified meiE as an aveE homolog in the meilingmycin gene cluster, annotated MeiE as a cytochrome P450 hydroxylase, and proposed that MeiE oxidizes the TE-released, cyclized polyketide to generate a C8-C6 furan ring, a C8 hydroxyl group, or C8 and C6 hydroxyl groups. reference https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes MIBiG BGC0000093.5 places meiE/AAM97314.1 inside the active Streptomyces nanchangensis meilingmycin biosynthetic gene cluster and links the CDS to MITE0000010 at 100% identity. reference https://www.uniprot.org/uniprotkb/Q6YL50 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:Q6YL50 resolves as a 459 aa Streptomyces nanchangensis meiE cytochrome P450 hydroxylase TrEMBL entry, cross-references EMBL FJ952082 protein AAM97314.1, annotates heme as a cofactor, and carries heme-binding and monooxygenase cross-references. reference https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes NCBI Protein AAM97314.1 is the Streptomyces nanchangensis MeiE cytochrome P450 hydroxylase encoded by meiE at complement(FJ952082.1:58480..59859).
MeiE cytochrome P450	forms	heterocyclized meilingmycin intermediate	This product corresponds to MITE reaction 3's heterocyclized meilingmycin intermediate structure.	https://mite.bioinformatics.nl/repository/MITE0000010 DOI:10.1128/AEM.02262-09 https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk https://www.uniprot.org/uniprotkb/Q6YL50 https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 reference https://mite.bioinformatics.nl/repository/MITE0000010 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Heterocyclization, Hydroxylation notes MITE0000010 is active, lists three knock-out-supported MeiE reaction rows on meilingmycin/milbemycin scaffolds, assigns two rows to hydroxylation and one row to heterocyclization, lists heme and iron as cofactors, links each reaction row to DOI:10.1128/AEM.02262-09, and links MeiE to UniProtKB:Q6YL50, GenPept AAM97314.1, and MIBiG BGC0000093. reference DOI:10.1128/AEM.02262-09 evidence type PRIMARY_EXPERIMENT notes He et al. identified meiE as an aveE homolog in the meilingmycin gene cluster, annotated MeiE as a cytochrome P450 hydroxylase, and proposed that MeiE oxidizes the TE-released, cyclized polyketide to generate a C8-C6 furan ring, a C8 hydroxyl group, or C8 and C6 hydroxyl groups. reference https://mibig.secondarymetabolites.org/repository/BGC0000093.5/BGC0000093.gbk evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes MIBiG BGC0000093.5 places meiE/AAM97314.1 inside the active Streptomyces nanchangensis meilingmycin biosynthetic gene cluster and links the CDS to MITE0000010 at 100% identity. reference https://www.uniprot.org/uniprotkb/Q6YL50 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:Q6YL50 resolves as a 459 aa Streptomyces nanchangensis meiE cytochrome P450 hydroxylase TrEMBL entry, cross-references EMBL FJ952082 protein AAM97314.1, annotates heme as a cofactor, and carries heme-binding and monooxygenase cross-references. reference https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes NCBI Protein AAM97314.1 is the Streptomyces nanchangensis MeiE cytochrome P450 hydroxylase encoded by meiE at complement(FJ952082.1:58480..59859).
heme	serves as cofactor for	MeiE cytochrome P450	MITE lists heme as MeiE's organic cofactor and UniProt annotates heme binding for Q6YL50.	https://mite.bioinformatics.nl/repository/MITE0000010 https://www.uniprot.org/uniprotkb/Q6YL50 https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 reference https://mite.bioinformatics.nl/repository/MITE0000010 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 snippet Heterocyclization, Hydroxylation notes MITE0000010 is active, lists three knock-out-supported MeiE reaction rows on meilingmycin/milbemycin scaffolds, assigns two rows to hydroxylation and one row to heterocyclization, lists heme and iron as cofactors, links each reaction row to DOI:10.1128/AEM.02262-09, and links MeiE to UniProtKB:Q6YL50, GenPept AAM97314.1, and MIBiG BGC0000093. reference https://www.uniprot.org/uniprotkb/Q6YL50 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes UniProtKB:Q6YL50 resolves as a 459 aa Streptomyces nanchangensis meiE cytochrome P450 hydroxylase TrEMBL entry, cross-references EMBL FJ952082 protein AAM97314.1, annotates heme as a cofactor, and carries heme-binding and monooxygenase cross-references. reference https://www.ncbi.nlm.nih.gov/protein/AAM97314.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-02 notes NCBI Protein AAM97314.1 is the Streptomyces nanchangensis MeiE cytochrome P450 hydroxylase encoded by meiE at complement(FJ952082.1:58480..59859).

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (meilingmycin) ↗ MediaIngredientMech (naturalproductmech:mibig-13e3c54c7c) ↗ TaxonMech: Streptomyces nanchangensis (NCBITaxon:204925) ↗ PathwayMech (mibig:BGC0000093) ↗

Provenance

Source concept	Source	Version
BGC0000093	MIBIG	5

This page is generated from data/natural_products/polyketides/meilingmycin.yaml, which is generated from the committed inventories. Neither is edited by hand.