nataxazole

naturalproductmech:mibig-6f9e5ea5f0 MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	SFONMRKWCPHKLA-UHFFFAOYSA-N
SMILES	COC(=O)C1=C2N=C(OC2=CC=C1)C1=C2N=C(OC2=CC=C1)C1=C(C)C=CC=C1O
Stereochemistry	fully defined
Cross-references	npatlas:NPA019425

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Streptomyces sp. Tu 6176 NCBITaxon:1470557	SOURCE_ASSERTION	mibig:BGC0001213	PMID:25892546

Occurrences 3

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

Taxon	Source	Reference
Streptomyces NCBITaxon:1883	LOTUS	DOI:10.1002/CBIC.201500153
Streptomyces NCBITaxon:1883	LOTUS	DOI:10.1002/CBIC.201500261
Streptomyces NCBITaxon:1883	LOTUS	DOI:10.1038/JA.2008.97

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0001213	Streptomyces sp. Tu 6176 NCBITaxon:1470557	CLUSTER_UNSTATED	genbank:KK106988.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Causal graph — biosynthesis 15 nodes, 14 edges

Partial map of twelve native protein references explicitly annotated for nataxazole biosynthesis in GenBank LN713864.1, not a reconstructed sequence of experimentally demonstrated reactions. NatAM and NatL2 are grounded to exact UniProt/native sequences and checked against independent PDB deposits; the other ten references use verified GenPept identifiers without substituting homologs. All participation edges are database assertions, and NatAC2 remains proposed. The core-cluster association does not establish conversion to the exact final methyl ester: no nataxazole product endpoint, producer upgrade or complete-pathway claim is added. Native regulatory and transport candidates remain outside this core-protein mechanism; the functionless NatX annotation is not promoted.

Subject	Predicate	Object	Notes	Evidence
natP reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42816.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natP CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42816.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet 4'-phosphopantetheinyl transferase notes GenPept CEK42816.1, natP, 236 aa, coded by complement(LN713864.1:16307..17017); source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natAL reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42817.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natAL CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42817.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet 3-deoxy-D-arabinose-heptulosonic 7-phosphate synthase notes GenPept CEK42817.1, natAL, 460 aa, coded by complement(LN713864.1:17014..18396); source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natAN reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42818.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natAN CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42818.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Anthranilate synthase notes GenPept CEK42818.1, natAN, 636 aa, coded by complement(LN713864.1:18393..20303); source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natIS reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42819.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazol biosynthesis notes The deposited natIS CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42819.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Isochorismatase notes GenPept CEK42819.1, natIS, 230 aa, coded by complement(LN713864.1:20300..20992); source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natDB reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42820.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natDB CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42820.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet 2,3-dihydro-3-hydroxyanthranilate dehydrogenase notes GenPept CEK42820.1, natDB, 274 aa, coded by complement(LN713864.1:21340..22164); source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natAM reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42821.1 https://www.uniprot.org/uniprotkb/A0A022MQ12/entry DOI:10.2210/pdb6SJ2/pdb reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natAM CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42821.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Amidohydrolase notes GenPept CEK42821.1, natAM, 494 aa, coded by complement(LN713864.1:22161..23645); source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction. reference https://www.uniprot.org/uniprotkb/A0A022MQ12/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Unreviewed UniProtKB A0A022MQ12: gene natAM, taxon 1470557, exact native CEK42821.1 sequence and LN713864 cross-reference. The entry has no curated reaction comments. This is the matching native protein, not a convenient homolog; native accession identity is separate from construct identity. reference DOI:10.2210/pdb6SJ2/pdb evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes NatAM is 494 aa; PDB 6SJ2 entity 1 is 519 aa, with a 25-aa N-terminal extension MSYYHHHHHHDYDIPTTENLYFQGA and an unchanged native sequence at positions 26-519 in chains A/B. The deposit annotates Streptomyces albus as expression host; that metadata is not independently resolved against the uninspected methods. The independent PDB coordinate dataset and reference alignment were inspected for identity. DOI:10.1002/anie.201915685 is its cited primary paper, not a claimed full-paper read. The deposited 3-hydroxyanthranilic-acid ligand is not nataxazole, and no final-product binding, catalytic conversion or NatAM-NatL2 complex is inferred.
natAC1 reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42822.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natAC1 CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42822.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Acyl carrier protein notes GenPept CEK42822.1, natAC1, 89 aa, coded by complement(LN713864.1:23645..23914); source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natL1 reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42823.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natL1 CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42823.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet AMP-dependent synthetase and ligase notes GenPept CEK42823.1, natL1, 539 aa, coded by complement(LN713864.1:23916..25535); source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natS reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42824.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natS CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42824.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet 3-oxoacyl-ACP synthase III notes GenPept CEK42824.1, natS, 350 aa, coded by complement(LN713864.1:25528..26580); source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natL2 reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42825.1 https://www.uniprot.org/uniprotkb/A0A022MRT4/entry DOI:10.2210/pdb6SIY/pdb reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natL2 CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42825.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet AMP-dependent synthetase and ligase notes GenPept CEK42825.1, natL2, 436 aa, coded by LN713864.1:27193..28503; source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction. reference https://www.uniprot.org/uniprotkb/A0A022MRT4/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Unreviewed UniProtKB A0A022MRT4: gene natL2, taxon 1470557, exact native CEK42825.1 sequence and LN713864 cross-reference. The entry has no curated reaction comments. This is the matching native protein, not a convenient homolog; native accession identity is separate from construct identity. reference DOI:10.2210/pdb6SIY/pdb evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes NatL2 is 436 aa; PDB 6SIY entity 1 is the identical 436-aa deposited polymer in chains A/B, without a deposited extension or native substitution. The deposit annotates Escherichia coli BL21 as expression host. This does not prove that every functional-assay construct lacked tags. The independent PDB coordinate dataset and reference alignment were inspected for identity. DOI:10.1002/anie.201915685 is its cited primary paper, not a claimed full-paper read. The deposited 3-hydroxyanthranilic-acid ligand is not nataxazole, and no final-product binding, catalytic conversion or NatAM-NatL2 complex is inferred.
natPK reference protein (Tu 6176)	is annotated to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42827.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis notes The deposited natPK CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42827.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet 6-methylsalicylic acid synthase, type I PKS notes GenPept CEK42827.1, natPK, 1717 aa, coded by LN713864.1:29077..34230; source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natAC2 reference protein (Tu 6176)	is proposed to participate in	nataxazole-associated core biosynthesis (partial, source-annotated)	Database-backed biosynthetic participation only. No reaction order, exact substrate/product pair, catalytic rate, deletion phenotype or essentiality is asserted. The protein node identifies the native reference, not an unspecified engineered construct. NatAC2 remains explicitly proposed in the source. See curator-nataxazole-reaction-and-product-scope for the final-product and mechanism limits.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42831.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Proposed nataxazole biosynthesis notes The deposited natAC2 CDS explicitly carries this function qualifier; the association is not inferred from adjacency alone. This is a source annotation, not independent verification of an enzyme reaction, necessity or sufficiency. LN713864.1 cites PMID:25892546; the full paper was not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42831.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Acyl carrier protein notes GenPept CEK42831.1, natAC2, 88 aa, coded by LN713864.1:40190..40456; source Streptomyces sp. Tu 6176, taxon 1470557. GenPept sequence, native CDS translation and an independent genetic-code-11 translation agree exactly. The product name is deposited nomenclature, not an adopted substrate-specific reaction.
natS reference protein (Tu 6176)	has a sequence-identical UniProtKB example	natS (Streptomyces sp. Tu 6176 UniProtKB example)	UniProtKB:A0A022MLV4 is the active Streptomyces sp. Tu 6176 natS entry sharing 100% amino-acid sequence identity (UPI000369AEC3) with LN849795.1 CEK42824.1.	https://www.uniprot.org/uniprotkb/A0A022MLV4 https://www.uniprot.org/uniparc/UPI000369AEC3 https://www.ncbi.nlm.nih.gov/protein/CEK42824.1 reference https://www.uniprot.org/uniprotkb/A0A022MLV4 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes Active Streptomyces sp. Tu 6176 UniProtKB entry (A0A022MLV4, natS) for the UPI000369AEC3 sequence. reference https://www.uniprot.org/uniparc/UPI000369AEC3 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes UniParc UPI000369AEC3 links LN849795.1 CEK42824.1 (natS) and active UniProtKB:A0A022MLV4 under a single 100%-identical sequence. reference https://www.ncbi.nlm.nih.gov/protein/CEK42824.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes LN849795.1 CEK42824.1 is the Streptomyces sp. Tu 6176 natS coding sequence.
natAC2 reference protein (Tu 6176)	has a sequence-identical UniProtKB example	natAC2 (S. olivoverticillatus Tu 6074 UniProtKB example)	UniProtKB:A0ABV8TEK9 (Streptomyces olivoverticillatus Tu 6074) shares 100% amino-acid sequence identity (UPI0003645395) with Streptomyces sp. Tu 6176 natAC2 (CEK42831.1).	https://www.uniprot.org/uniprotkb/A0ABV8TEK9 https://www.uniprot.org/uniparc/UPI0003645395 https://www.ncbi.nlm.nih.gov/protein/CEK42831.1 reference https://www.uniprot.org/uniprotkb/A0ABV8TEK9 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes Active Streptomyces olivoverticillatus Tu 6074 UniProtKB entry (A0ABV8TEK9) for the UPI0003645395 acyl carrier protein sequence. reference https://www.uniprot.org/uniparc/UPI0003645395 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes UniParc UPI0003645395 links LN849795.1 CEK42831.1 (natAC2) and active UniProtKB:A0ABV8TEK9 under a single 100%-identical sequence. reference https://www.ncbi.nlm.nih.gov/protein/CEK42831.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes LN849795.1 CEK42831.1 is the Streptomyces sp. Tu 6176 natAC2 coding sequence.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 13 nodes, 12 edges

Native cluster-associated regulation and proposed transport, separate from the core biosynthesis-protein map. Eight versioned GenPept references are supported by explicit LN713864.1 annotations and exact native DNA/protein sequence checks. All edges are database assertions: NatR1-4 are annotated regulators without an adopted regulatory sign or direct target, and NatT1-4 remain proposed transporters without a demonstrated substrate or direction. The common process groups source-associated roles; it is not a physical complex, ordered reaction sequence or proof that these proteins produce or export the exact final nataxazole methyl ester. No bioactivity target or self-resistance mechanism is asserted. Versioned native identifiers are used without substituting homologs or unaudited UniProt accessions.

Subject	Predicate	Object	Notes	Evidence
natR1 reference protein (Tu 6176)	has an annotated regulatory role in	nataxazole-associated biosynthetic system (source-annotated, partial)	No individual essentiality, complete pathway, final-product sufficiency, producer-grade upgrade or physical protein complex is asserted. No activating or repressing sign, direct DNA binding, target promoter, transcriptional response or yield change is adopted from this annotation. See curator-nataxazole-regulation-and-transport-scope for the unresolved mechanism.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42811.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis cluster regulator notes The deposited CDS explicitly carries this function qualifier; the association is not inferred from gene neighborhood alone. This is a database annotation, not a direct functional experiment. LN713864.1 cites PMID:25892546; its full paper and supplements were not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42811.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet LuxR-family transcriptional regulator notes GenPept CEK42811.1, natR1, 935 aa, coded by complement(LN713864.1:6125..8932); native Streptomyces sp. Tu 6176, taxon 1470557. The independent GenPept sequence, native CDS annotation and genetic-code-11 DNA translation agree exactly. Gene, product and function qualifiers also match. The accession identifies the native reference, not an unspecified overexpression plasmid, tagged construct or homolog.
natR2 reference protein (Tu 6176)	has an annotated regulatory role in	nataxazole-associated biosynthetic system (source-annotated, partial)	No individual essentiality, complete pathway, final-product sufficiency, producer-grade upgrade or physical protein complex is asserted. No activating or repressing sign, direct DNA binding, target promoter, transcriptional response or yield change is adopted from this annotation. See curator-nataxazole-regulation-and-transport-scope for the unresolved mechanism.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42815.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis cluster regulator notes The deposited CDS explicitly carries this function qualifier; the association is not inferred from gene neighborhood alone. This is a database annotation, not a direct functional experiment. LN713864.1 cites PMID:25892546; its full paper and supplements were not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42815.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet TetR-family transcriptional regulator notes GenPept CEK42815.1, natR2, 198 aa, coded by LN713864.1:15560..16156; native Streptomyces sp. Tu 6176, taxon 1470557. The independent GenPept sequence, native CDS annotation and genetic-code-11 DNA translation agree exactly. Gene, product and function qualifiers also match. The accession identifies the native reference, not an unspecified overexpression plasmid, tagged construct or homolog.
natR3 reference protein (Tu 6176)	has an annotated regulatory role in	nataxazole-associated biosynthetic system (source-annotated, partial)	No individual essentiality, complete pathway, final-product sufficiency, producer-grade upgrade or physical protein complex is asserted. No activating or repressing sign, direct DNA binding, target promoter, transcriptional response or yield change is adopted from this annotation. See curator-nataxazole-regulation-and-transport-scope for the unresolved mechanism.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42828.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis cluster regulator notes The deposited CDS explicitly carries this function qualifier; the association is not inferred from gene neighborhood alone. This is a database annotation, not a direct functional experiment. LN713864.1 cites PMID:25892546; its full paper and supplements were not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42828.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet TetR-family transcriptional regulator notes GenPept CEK42828.1, natR3, 232 aa, coded by complement(LN713864.1:34517..35215); native Streptomyces sp. Tu 6176, taxon 1470557. The independent GenPept sequence, native CDS annotation and genetic-code-11 DNA translation agree exactly. Gene, product and function qualifiers also match. The accession identifies the native reference, not an unspecified overexpression plasmid, tagged construct or homolog.
natR4 reference protein (Tu 6176)	has an annotated regulatory role in	nataxazole-associated biosynthetic system (source-annotated, partial)	No individual essentiality, complete pathway, final-product sufficiency, producer-grade upgrade or physical protein complex is asserted. No activating or repressing sign, direct DNA binding, target promoter, transcriptional response or yield change is adopted from this annotation. See curator-nataxazole-regulation-and-transport-scope for the unresolved mechanism.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42830.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Nataxazole biosynthesis cluter regulator notes The deposited CDS explicitly carries this function qualifier; the association is not inferred from gene neighborhood alone. This is a database annotation, not a direct functional experiment. LN713864.1 cites PMID:25892546; its full paper and supplements were not inspected. The source spelling "cluter" is retained verbatim. reference https://www.ncbi.nlm.nih.gov/protein/CEK42830.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet SARP-family trancriptional regulator notes GenPept CEK42830.1, natR4, 845 aa, coded by LN713864.1:37329..39866; native Streptomyces sp. Tu 6176, taxon 1470557. The independent GenPept sequence, native CDS annotation and genetic-code-11 DNA translation agree exactly. Gene, product and function qualifiers also match. The accession identifies the native reference, not an unspecified overexpression plasmid, tagged construct or homolog.
natT1 reference protein (Tu 6176)	has a proposed transport role in	nataxazole-associated biosynthetic system (source-annotated, partial)	No individual essentiality, complete pathway, final-product sufficiency, producer-grade upgrade or physical protein complex is asserted. Transport involvement remains proposed, as in the source. No import or export direction, transported chemical form, flux, ATP dependence in this pathway, or self-resistance phenotype is established here. See curator-nataxazole-regulation-and-transport-scope for the unresolved mechanism.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42812.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Proposed nataxazole transporter notes The deposited CDS explicitly carries this function qualifier; the association is not inferred from gene neighborhood alone. This is a database annotation, not a direct functional experiment. LN713864.1 cites PMID:25892546; its full paper and supplements were not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42812.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet ABC transporter notes GenPept CEK42812.1, natT1, 579 aa, coded by complement(LN713864.1:9384..11123); native Streptomyces sp. Tu 6176, taxon 1470557. The independent GenPept sequence, native CDS annotation and genetic-code-11 DNA translation agree exactly. Gene, product and function qualifiers also match. The accession identifies the native reference, not an unspecified overexpression plasmid, tagged construct or homolog.
natT2 reference protein (Tu 6176)	has a proposed transport role in	nataxazole-associated biosynthetic system (source-annotated, partial)	No individual essentiality, complete pathway, final-product sufficiency, producer-grade upgrade or physical protein complex is asserted. Transport involvement remains proposed, as in the source. No import or export direction, transported chemical form, flux, ATP dependence in this pathway, or self-resistance phenotype is established here. See curator-nataxazole-regulation-and-transport-scope for the unresolved mechanism.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42813.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Proposed nataxazole transporter notes The deposited CDS explicitly carries this function qualifier; the association is not inferred from gene neighborhood alone. This is a database annotation, not a direct functional experiment. LN713864.1 cites PMID:25892546; its full paper and supplements were not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42813.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet ABC transporter notes GenPept CEK42813.1, natT2, 608 aa, coded by complement(LN713864.1:11123..12949); native Streptomyces sp. Tu 6176, taxon 1470557. The independent GenPept sequence, native CDS annotation and genetic-code-11 DNA translation agree exactly. Gene, product and function qualifiers also match. The accession identifies the native reference, not an unspecified overexpression plasmid, tagged construct or homolog.
natT3 reference protein (Tu 6176)	has a proposed transport role in	nataxazole-associated biosynthetic system (source-annotated, partial)	No individual essentiality, complete pathway, final-product sufficiency, producer-grade upgrade or physical protein complex is asserted. Transport involvement remains proposed, as in the source. No import or export direction, transported chemical form, flux, ATP dependence in this pathway, or self-resistance phenotype is established here. See curator-nataxazole-regulation-and-transport-scope for the unresolved mechanism.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42814.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Proposed nataxazole transporter notes The deposited CDS explicitly carries this function qualifier; the association is not inferred from gene neighborhood alone. This is a database annotation, not a direct functional experiment. LN713864.1 cites PMID:25892546; its full paper and supplements were not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42814.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet MMPL domain-containing transport protein notes GenPept CEK42814.1, natT3, 722 aa, coded by complement(LN713864.1:13128..15296); native Streptomyces sp. Tu 6176, taxon 1470557. The independent GenPept sequence, native CDS annotation and genetic-code-11 DNA translation agree exactly. Gene, product and function qualifiers also match. The accession identifies the native reference, not an unspecified overexpression plasmid, tagged construct or homolog.
natT4 reference protein (Tu 6176)	has a proposed transport role in	nataxazole-associated biosynthetic system (source-annotated, partial)	No individual essentiality, complete pathway, final-product sufficiency, producer-grade upgrade or physical protein complex is asserted. Transport involvement remains proposed, as in the source. No import or export direction, transported chemical form, flux, ATP dependence in this pathway, or self-resistance phenotype is established here. The deposited "Drug resistance transporter" product name is not a demonstrated nataxazole-resistance result or a compound target. See curator-nataxazole-regulation-and-transport-scope for the unresolved mechanism.	https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 https://www.ncbi.nlm.nih.gov/protein/CEK42829.1 reference https://www.ncbi.nlm.nih.gov/nuccore/LN713864.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Proposed nataxazole transporter notes The deposited CDS explicitly carries this function qualifier; the association is not inferred from gene neighborhood alone. This is a database annotation, not a direct functional experiment. LN713864.1 cites PMID:25892546; its full paper and supplements were not inspected. reference https://www.ncbi.nlm.nih.gov/protein/CEK42829.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Drug resistance transporter, EmrB/QacA-family transporter notes GenPept CEK42829.1, natT4, 515 aa, coded by LN713864.1:35535..37082; native Streptomyces sp. Tu 6176, taxon 1470557. The independent GenPept sequence, native CDS annotation and genetic-code-11 DNA translation agree exactly. Gene, product and function qualifiers also match. The accession identifies the native reference, not an unspecified overexpression plasmid, tagged construct or homolog.
natR2 reference protein (Tu 6176)	has a sequence-identical UniProtKB example	natR2 (S. olivoverticillatus Tu 6074 UniProtKB example)	UniProtKB:A0ABV8TEJ6 (Streptomyces olivoverticillatus Tu 6074) shares 100% amino-acid sequence identity (UPI0003661C80) with Streptomyces sp. Tu 6176 natR2 (CEK42815.1).	https://www.uniprot.org/uniprotkb/A0ABV8TEJ6 https://www.uniprot.org/uniparc/UPI0003661C80 https://www.ncbi.nlm.nih.gov/protein/CEK42815.1 reference https://www.uniprot.org/uniprotkb/A0ABV8TEJ6 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes Active Streptomyces olivoverticillatus Tu 6074 UniProtKB entry (A0ABV8TEJ6) for the UPI0003661C80 sequence. reference https://www.uniprot.org/uniparc/UPI0003661C80 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes UniParc UPI0003661C80 links LN849795.1 CEK42815.1 (natR2) and active UniProtKB:A0ABV8TEJ6 under a single 100%-identical sequence. reference https://www.ncbi.nlm.nih.gov/protein/CEK42815.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes LN849795.1 CEK42815.1 is the Streptomyces sp. Tu 6176 natR2 coding sequence.
natR3 reference protein (Tu 6176)	has a sequence-identical UniProtKB example	natR3 (S. olivoverticillatus Tu 6074 UniProtKB example)	UniProtKB:A0ABV8TFE0 (Streptomyces olivoverticillatus Tu 6074) shares 100% amino-acid sequence identity (UPI00036EBE62) with Streptomyces sp. Tu 6176 natR3 (CEK42828.1).	https://www.uniprot.org/uniprotkb/A0ABV8TFE0 https://www.uniprot.org/uniparc/UPI00036EBE62 https://www.ncbi.nlm.nih.gov/protein/CEK42828.1 reference https://www.uniprot.org/uniprotkb/A0ABV8TFE0 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes Active Streptomyces olivoverticillatus Tu 6074 UniProtKB entry (A0ABV8TFE0) for the UPI00036EBE62 sequence. reference https://www.uniprot.org/uniparc/UPI00036EBE62 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes UniParc UPI00036EBE62 links LN849795.1 CEK42828.1 (natR3) and active UniProtKB:A0ABV8TFE0 under a single 100%-identical sequence. reference https://www.ncbi.nlm.nih.gov/protein/CEK42828.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes LN849795.1 CEK42828.1 is the Streptomyces sp. Tu 6176 natR3 coding sequence.
natT1 reference protein (Tu 6176)	has a sequence-identical UniProtKB example	natT1 (S. olivoverticillatus Tu 6074 UniProtKB example)	UniProtKB:A0ABV8TFC5 (Streptomyces olivoverticillatus Tu 6074) shares 100% amino-acid sequence identity (UPI0004478404) with Streptomyces sp. Tu 6176 natT1 (CEK42812.1).	https://www.uniprot.org/uniprotkb/A0ABV8TFC5 https://www.uniprot.org/uniparc/UPI0004478404 https://www.ncbi.nlm.nih.gov/protein/CEK42812.1 reference https://www.uniprot.org/uniprotkb/A0ABV8TFC5 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes Active Streptomyces olivoverticillatus Tu 6074 UniProtKB entry (A0ABV8TFC5) for the UPI0004478404 sequence. reference https://www.uniprot.org/uniparc/UPI0004478404 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes UniParc UPI0004478404 links LN849795.1 CEK42812.1 (natT1) and active UniProtKB:A0ABV8TFC5 under a single 100%-identical sequence. reference https://www.ncbi.nlm.nih.gov/protein/CEK42812.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes LN849795.1 CEK42812.1 is the Streptomyces sp. Tu 6176 natT1 coding sequence.
natT4 reference protein (Tu 6176)	has a sequence-identical UniProtKB example	natT4 (S. olivoverticillatus Tu 6074 UniProtKB example)	UniProtKB:A0ABV8TEP5 (Streptomyces olivoverticillatus Tu 6074) shares 100% amino-acid sequence identity (UPI0004505055) with Streptomyces sp. Tu 6176 natT4 (CEK42829.1).	https://www.uniprot.org/uniprotkb/A0ABV8TEP5 https://www.uniprot.org/uniparc/UPI0004505055 https://www.ncbi.nlm.nih.gov/protein/CEK42829.1 reference https://www.uniprot.org/uniprotkb/A0ABV8TEP5 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes Active Streptomyces olivoverticillatus Tu 6074 UniProtKB entry (A0ABV8TEP5) for the UPI0004505055 sequence. reference https://www.uniprot.org/uniparc/UPI0004505055 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes UniParc UPI0004505055 links LN849795.1 CEK42829.1 (natT4) and active UniProtKB:A0ABV8TEP5 under a single 100%-identical sequence. reference https://www.ncbi.nlm.nih.gov/protein/CEK42829.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-17 notes LN849795.1 CEK42829.1 is the Streptomyces sp. Tu 6176 natT4 coding sequence.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (nataxazole) ↗ MediaIngredientMech (naturalproductmech:mibig-6f9e5ea5f0) ↗ TaxonMech: Streptomyces sp. Tu 6176 (NCBITaxon:1470557) ↗ PathwayMech (mibig:BGC0001213) ↗

Open questions 2

curator-nataxazole-reaction-and-product-scope — Which exact reactions and constructs establish the native core-protein roles, and which additional steps connect the core pathway to the exact nataxazole methyl ester?

curator-nataxazole-regulation-and-transport-scope — Which native regulatory targets and signs, and which transporter substrates and directions, are experimentally established for NatR1-4 and NatT1-4?

Provenance

Source concept	Source	Version
BGC0001213	MIBIG	5

This page is generated from data/natural_products/polyketides/nataxazole.yaml, which is generated from the committed inventories. Neither is edited by hand.