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nonactin

naturalproductmech:mibig-4e6eae01d4 MINTED SEEDED cytotoxicenzyme inhibitor

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.
What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKeyRMIXHJPMNBXMBU-QIIXEHPYSA-N
SMILESC[C@@H]1C[C@H]2CC[C@H](O2)[C@@H](C(=O)O[C@H](C[C@@H]3CC[C@@H](O3)[C@H](C(=O)O[C@@H](C[C@H]4CC[C@H](O4)[C@@H](C(=O)O[C@H](C[C@@H]5CC[C@@H](O5)[C@H](C(=O)O1)C)C)C)C)C)C)C
Stereochemistryfully defined
Cross-referencespubchem:72519

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 3

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Streptomyces griseus subsp. griseus
NCBITaxon:67263		 SOURCE_ASSERTION mibig:BGC0000243 PMID:10858335
Streptomyces griseus subsp. griseus
NCBITaxon:67263		 SOURCE_ASSERTION mibig:BGC0000244 PMID:10858335
Streptomyces griseus subsp. griseus
NCBITaxon:67263		 SOURCE_ASSERTION mibig:BGC0000252 PMID:10650222

Occurrences 8

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Streptomyces globisporus
NCBITaxon:1908		 LOTUS DOI:10.1007/BF02884062
Streptomyces griseus
NCBITaxon:1911		 LOTUS DOI:10.1021/JA016965F
Streptomyces griseus
NCBITaxon:1911		 LOTUS DOI:10.1021/NP100421V
Streptomyces griseus
NCBITaxon:1911		 LOTUS DOI:10.1039/C39850001568
Streptomyces griseus
NCBITaxon:1911		 LOTUS DOI:10.1039/P19880001719
Streptomyces griseus
NCBITaxon:1911		 LOTUS DOI:10.1039/P19890001461
Streptomyces griseus
NCBITaxon:1911		 LOTUS DOI:10.1128/AAC.44.7.1809-1817.2000
Streptomyces griseus
NCBITaxon:1911		 LOTUS DOI:10.1139/CJM-2016-0248

Biosynthetic gene clusters 3

AccessionHostLocus evidenceGenome
mibig:BGC0000243 Streptomyces griseus subsp. griseus
NCBITaxon:67263 		CLUSTER_UNSTATED genbank:AF263011.1
mibig:BGC0000244 Streptomyces griseus subsp. griseus
NCBITaxon:67263 		CLUSTER_UNSTATED genbank:AF263012.1
mibig:BGC0000252 Streptomyces griseus subsp. griseus
NCBITaxon:67263 		CLUSTER_UNSTATED genbank:AF074603.2

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 3 producers (0 causal) 8 cited occurrences BIOSYNTHETIC LOCUS 3 gene clusters 0 curated pathway steps NATURAL PRODUCT nonactin naturalproductmech:mibig-4e6eae01d4 Polyketides TARGETS & BIOACTIVITY 0 molecular targets 25 bioactivities · 0 graphs

Bioactivities 25

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

AssayResultTarget enzymeSource detail
Luminescence cell-based high throughput primary assay to identify inhibitors of TEAD-YAP interaction.ACTIVENot recorded
pubchem.aid:1259422
assay
Luminescence cell-based high throughput primary assay to identify inhibitors of TEAD-YAP interaction.
evidence
  • reference
    pubchem.aid:1259422
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1259422, deposited by The Scripps Research Institute Molecular Screening Center; assay type Screening. Target accession NP_001123617 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
activity class
ENZYME_INHIBITOR
NCI In Vivo Anticancer Drug Screen. Data for tumor model Adenocarcinoma 755 (subcutaneous) in B6D2F1 (BDF1) miceACTIVENot recorded
pubchem.aid:180
assay
NCI In Vivo Anticancer Drug Screen. Data for tumor model Adenocarcinoma 755 (subcutaneous) in B6D2F1 (BDF1) mice
evidence
  • reference
    pubchem.aid:180
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 180, deposited by DTP/NCI; assay type Other.
call
ACTIVE
activity class
CYTOTOXIC
NCI In Vivo Anticancer Drug Screen. Data for tumor model Sarcoma 180 (subcutaneous) in Swiss miceACTIVENot recorded
pubchem.aid:342
assay
NCI In Vivo Anticancer Drug Screen. Data for tumor model Sarcoma 180 (subcutaneous) in Swiss mice
evidence
  • reference
    pubchem.aid:342
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 342, deposited by DTP/NCI; assay type Other.
call
ACTIVE
activity class
CYTOTOXIC
NCI Yeast Anticancer Drug Screen. Data for the bub3 strainACTIVENot recorded
pubchem.aid:167
assay
NCI Yeast Anticancer Drug Screen. Data for the bub3 strain
evidence
  • reference
    pubchem.aid:167
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 167, deposited by DTP/NCI; assay type Other.
call
ACTIVE
activity class
CYTOTOXIC
NCI Yeast Anticancer Drug Screen. Data for the cln2 rad14 strainACTIVENot recorded
pubchem.aid:165
assay
NCI Yeast Anticancer Drug Screen. Data for the cln2 rad14 strain
evidence
  • reference
    pubchem.aid:165
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 165, deposited by DTP/NCI; assay type Other.
call
ACTIVE
activity class
CYTOTOXIC
NCI Yeast Anticancer Drug Screen. Data for the mec2-1 strainACTIVENot recorded
pubchem.aid:157
assay
NCI Yeast Anticancer Drug Screen. Data for the mec2-1 strain
evidence
  • reference
    pubchem.aid:157
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 157, deposited by DTP/NCI; assay type Other.
call
ACTIVE
activity class
CYTOTOXIC
NCI Yeast Anticancer Drug Screen. Data for the mlh1 rad18 strainACTIVENot recorded
pubchem.aid:175
assay
NCI Yeast Anticancer Drug Screen. Data for the mlh1 rad18 strain
evidence
  • reference
    pubchem.aid:175
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 175, deposited by DTP/NCI; assay type Other.
call
ACTIVE
activity class
CYTOTOXIC
NCI Yeast Anticancer Drug Screen. Data for the rad50 strainACTIVENot recorded
pubchem.aid:155
assay
NCI Yeast Anticancer Drug Screen. Data for the rad50 strain
evidence
  • reference
    pubchem.aid:155
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 155, deposited by DTP/NCI; assay type Other.
call
ACTIVE
activity class
CYTOTOXIC
NCI Yeast Anticancer Drug Screen. Data for the sgs1 mgt1 strainACTIVENot recorded
pubchem.aid:161
assay
NCI Yeast Anticancer Drug Screen. Data for the sgs1 mgt1 strain
evidence
  • reference
    pubchem.aid:161
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 161, deposited by DTP/NCI; assay type Other.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the A549/ATCC Non-Small Cell Lung cell lineACTIVENot recorded
pubchem.aid:19
assay
NCI human tumor cell line growth inhibition assay. Data for the A549/ATCC Non-Small Cell Lung cell line
evidence
  • reference
    pubchem.aid:19
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 19, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the ACHN Renal cell lineACTIVENot recorded
pubchem.aid:139
assay
NCI human tumor cell line growth inhibition assay. Data for the ACHN Renal cell line
evidence
  • reference
    pubchem.aid:139
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 139, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the BT-549 Breast cell lineACTIVENot recorded
pubchem.aid:89
assay
NCI human tumor cell line growth inhibition assay. Data for the BT-549 Breast cell line
evidence
  • reference
    pubchem.aid:89
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 89, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the CCRF-CEM Leukemia cell lineACTIVENot recorded
pubchem.aid:119
assay
NCI human tumor cell line growth inhibition assay. Data for the CCRF-CEM Leukemia cell line
evidence
  • reference
    pubchem.aid:119
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 119, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the COLO 205 Colon cell lineACTIVENot recorded
pubchem.aid:67
assay
NCI human tumor cell line growth inhibition assay. Data for the COLO 205 Colon cell line
evidence
  • reference
    pubchem.aid:67
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 67, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the EKVX Non-Small Cell Lung cell lineACTIVENot recorded
pubchem.aid:21
assay
NCI human tumor cell line growth inhibition assay. Data for the EKVX Non-Small Cell Lung cell line
evidence
  • reference
    pubchem.aid:21
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 21, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the HCT-116 Colon cell lineACTIVENot recorded
pubchem.aid:79
assay
NCI human tumor cell line growth inhibition assay. Data for the HCT-116 Colon cell line
evidence
  • reference
    pubchem.aid:79
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 79, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the HL-60(TB) Leukemia cell lineACTIVENot recorded
pubchem.aid:125
assay
NCI human tumor cell line growth inhibition assay. Data for the HL-60(TB) Leukemia cell line
evidence
  • reference
    pubchem.aid:125
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 125, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the HOP-62 Non-Small Cell Lung cell lineACTIVENot recorded
pubchem.aid:9
assay
NCI human tumor cell line growth inhibition assay. Data for the HOP-62 Non-Small Cell Lung cell line
evidence
  • reference
    pubchem.aid:9
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 9, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the HOP-92 Non-Small Cell Lung cell lineACTIVENot recorded
pubchem.aid:13
assay
NCI human tumor cell line growth inhibition assay. Data for the HOP-92 Non-Small Cell Lung cell line
evidence
  • reference
    pubchem.aid:13
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 13, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the HT29 Colon cell lineACTIVENot recorded
pubchem.aid:65
assay
NCI human tumor cell line growth inhibition assay. Data for the HT29 Colon cell line
evidence
  • reference
    pubchem.aid:65
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 65, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the IGROV1 Ovarian cell lineACTIVENot recorded
pubchem.aid:101
assay
NCI human tumor cell line growth inhibition assay. Data for the IGROV1 Ovarian cell line
evidence
  • reference
    pubchem.aid:101
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 101, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the K-562 Leukemia cell lineACTIVENot recorded
pubchem.aid:121
assay
NCI human tumor cell line growth inhibition assay. Data for the K-562 Leukemia cell line
evidence
  • reference
    pubchem.aid:121
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 121, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the KM12 Colon cell lineACTIVENot recorded
pubchem.aid:73
assay
NCI human tumor cell line growth inhibition assay. Data for the KM12 Colon cell line
evidence
  • reference
    pubchem.aid:73
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 73, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the LOX IMVI Melanoma cell lineACTIVENot recorded
pubchem.aid:23
assay
NCI human tumor cell line growth inhibition assay. Data for the LOX IMVI Melanoma cell line
evidence
  • reference
    pubchem.aid:23
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 23, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
NCI human tumor cell line growth inhibition assay. Data for the MALME-3M Melanoma cell lineACTIVENot recorded
pubchem.aid:29
assay
NCI human tumor cell line growth inhibition assay. Data for the MALME-3M Melanoma cell line
evidence
  • reference
    pubchem.aid:29
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 29, deposited by DTP/NCI; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
notes
29 further PubChem assay results are in data/raw/pubchem_bioassay.tsv but not written here.

Explore across X-Mech

AntibioticMech (nonactin) ↗ MediaIngredientMech (naturalproductmech:mibig-4e6eae01d4) ↗ TaxonMech: Streptomyces griseus subsp. griseus (NCBITaxon:67263) ↗ TaxonMech: Streptomyces griseus subsp. griseus (NCBITaxon:67263) ↗ TaxonMech: Streptomyces griseus subsp. griseus (NCBITaxon:67263) ↗ PathwayMech (mibig:BGC0000243) ↗ PathwayMech (mibig:BGC0000244) ↗ PathwayMech (mibig:BGC0000252) ↗

Open questions 1

shared-structure — This structure is reported by more than one MIBiG entry: BGC0000243, BGC0000244, BGC0000252. Confirm they describe the same compound rather than an upstream cross-reference error.

Provenance

Source conceptSourceVersion
BGC0000243MIBIG4
BGC0000244MIBIG4
BGC0000252MIBIG5

This page is generated from data/natural_products/polyketides/nonactin.yaml, which is generated from the committed inventories. Neither is edited by hand.