NaturalProductMech

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trypacidin

naturalproductmech:mibig-cfc470b905 MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.
What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKeyKMZYINVXZDQCKC-UHFFFAOYSA-N
SMILESCC1=CC2=C(C(=C1)OC)C(=O)C3(O2)C(=CC(=O)C=C3OC)C(=O)OC
Stereochemistry undefined stereocentres — the InChIKey does not distinguish this compound from its stereoisomers
Cross-referencespubchem:3035292

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Aspergillus fumigatus Af293
NCBITaxon:330879		 SOURCE_ASSERTION mibig:BGC0001403 DOI:10.1007/s13205-022-03194-3

Occurrences 3

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Rumex
NCBITaxon:3618		 LOTUS DOI:10.1016/J.BMCL.2013.04.059
Aspergillus fumigatus
NCBITaxon:746128		 LOTUS DOI:10.1111/1462-2920.13007
Aspergillus fumigatus
NCBITaxon:746128		 LOTUS DOI:10.3891/ACTA.CHEM.SCAND.19-0528

Biosynthetic gene clusters 1

AccessionHostLocus evidenceGenome
mibig:BGC0001403 Aspergillus fumigatus Af293
NCBITaxon:330879 		CLUSTER_UNSTATED genbank:CM000172.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 1 producer (0 causal) 3 cited occurrences BIOSYNTHETIC LOCUS 1 gene cluster 0 curated pathway steps NATURAL PRODUCT trypacidin naturalproductmech:mibig-cfc470b905 Polyketides TARGETS & BIOACTIVITY 0 molecular targets 0 bioactivities · 2 graphs

Causal graph — biosynthesis 32 nodes, 30 edges

Partial catalytic chemistry component; the atrochrysone-to-emodin-anthrone connector is intentionally omitted. Across this owner's two components: Sequence-verified native Af293 references with explicitly database-level trypacidin roles, not verified experimental alleles. Ten enzyme annotations and two regulators are represented. TpcB/G/I catalytic roles remain similarity-derived. Named intermediates are not exact chemical-species groundings or balanced equations. No atrochrysone-to-emodin-anthrone connector is asserted, so this is not a complete reconstituted pathway. The database spelling monomethylsulfochrin and the product's unspecified stereochemistry remain unresolved. Genetic loss and impairment are distinguished; no direct promoter binding, physical complex, bioactivity target, virulence mechanism, universal essentiality or producer-grade upgrade is asserted. TpcF is held without a catalytic assignment.

SubjectPredicateObjectNotesEvidence
TpcC (Af293 reference) has_database_annotated_catalytic_role_in Polyketide scaffold assembly (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQZ5/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89339.1
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ5/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 119, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:64232; physiological left-to-right RHEA:64233. Catalytic annotation ECO:0000305, PMID:26242966. The scaffold remains TpcC-bound; the free acid is released by TpcB, not by TpcC alone. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    synthesizes the scaffold of atrochrysone carboxylic acid from malonyl-CoA
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89339.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcC/AFUA_4G14560, 1782 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: join(AAHF01000005.1:81245..81594, AAHF01000005.1:81641..81941,AAHF01000005.1:81987..83099, AAHF01000005.1:83146..85512,AAHF01000005.1:85558..86775). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
Malonyl-CoA is_named_substrate_of Polyketide scaffold assembly (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ5/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ5/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 119, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:64232; physiological left-to-right RHEA:64233. Catalytic annotation ECO:0000305, PMID:26242966. The scaffold remains TpcC-bound; the free acid is released by TpcB, not by TpcC alone. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Polyketide scaffold assembly (database annotation) has_named_product TpcC-bound atrochrysone carboxyl thioester Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ5/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ5/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 119, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:64232; physiological left-to-right RHEA:64233. Catalytic annotation ECO:0000305, PMID:26242966. The scaffold remains TpcC-bound; the free acid is released by TpcB, not by TpcC alone. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcB (Af293 reference) has_similarity_inferred_catalytic_role_in Thioester hydrolysis and scaffold release (by similarity) Inferred by similarity, not directly demonstrated for this native sequence. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQZ6/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89338.1
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ6/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 85, sequence version 1. Inferred by similarity, not directly demonstrated for this native sequence. Catalytic annotation is transferred by similarity (ECO:0000250, UniProtKB:Q0CCY4): RHEA:64236, left-to-right RHEA:64237. Functional/genetic association does not independently demonstrate this exact native reaction. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    breaks the thioester bond to release the atrochrysone carboxylic acid
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89338.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcB/AFUA_4G14570, 421 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: complement(AAHF01000005.1:79841..81106). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
TpcC-bound atrochrysone carboxyl thioester is_named_substrate_of Thioester hydrolysis and scaffold release (by similarity) Inferred by similarity, not directly demonstrated for this native sequence. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ6/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ6/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 85, sequence version 1. Inferred by similarity, not directly demonstrated for this native sequence. Catalytic annotation is transferred by similarity (ECO:0000250, UniProtKB:Q0CCY4): RHEA:64236, left-to-right RHEA:64237. Functional/genetic association does not independently demonstrate this exact native reaction. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Thioester hydrolysis and scaffold release (by similarity) has_named_product Atrochrysone carboxylic acid Inferred by similarity, not directly demonstrated for this native sequence. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ6/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ6/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 85, sequence version 1. Inferred by similarity, not directly demonstrated for this native sequence. Catalytic annotation is transferred by similarity (ECO:0000250, UniProtKB:Q0CCY4): RHEA:64236, left-to-right RHEA:64237. Functional/genetic association does not independently demonstrate this exact native reaction. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcK (Af293 reference) has_database_annotated_catalytic_role_in Atrochrysone-carboxylate decarboxylation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQY7/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89347.1
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY7/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 74, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:64264, left-to-right RHEA:64265, ECO:0000269 attributed to PMID:31028901, DOI:10.1016/j.ymben.2019.04.008. That engineered-yeast primary paper was not inspected. The database reaction specifies (3R) species; this graph leaves intermediate stereochemistry ungrounded and does not assert an assayed Af293 allele. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    decarboxylation of atrochrysone carboxylic acid to yield atrochrysone
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89347.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcK/AFUA_4G14470, 142 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 1: Evidence at protein level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: complement(join(AAHF01000005.1:98952..99018, AAHF01000005.1:99070..99266,AAHF01000005.1:99314..99478)). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
Atrochrysone carboxylic acid is_named_substrate_of Atrochrysone-carboxylate decarboxylation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY7/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY7/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 74, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:64264, left-to-right RHEA:64265, ECO:0000269 attributed to PMID:31028901, DOI:10.1016/j.ymben.2019.04.008. That engineered-yeast primary paper was not inspected. The database reaction specifies (3R) species; this graph leaves intermediate stereochemistry ungrounded and does not assert an assayed Af293 allele. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Atrochrysone-carboxylate decarboxylation (database annotation) has_named_product Atrochrysone Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY7/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY7/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 74, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:64264, left-to-right RHEA:64265, ECO:0000269 attributed to PMID:31028901, DOI:10.1016/j.ymben.2019.04.008. That engineered-yeast primary paper was not inspected. The database reaction specifies (3R) species; this graph leaves intermediate stereochemistry ungrounded and does not assert an assayed Af293 allele. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcL (Af293 reference) has_database_annotated_catalytic_role_in Emodin-anthrone oxidation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQY6/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89348.2
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY6/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 73, sequence version 2. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:64268, left-to-right RHEA:64269; ECO:0000305 attributed to PMID:26242966. The atrochrysone-to-emodin-anthrone connector is deliberately absent: the shared function paragraph does not justify an exact enzyme or transformation here. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    oxidation of emodinanthrone to yield emodin
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89348.2
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcL/AFUA_4G14480, 161 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: join(AAHF01000005.1:99753..99819, AAHF01000005.1:99870..100288). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
Emodin anthrone is_named_substrate_of Emodin-anthrone oxidation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY6/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY6/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 73, sequence version 2. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:64268, left-to-right RHEA:64269; ECO:0000305 attributed to PMID:26242966. The atrochrysone-to-emodin-anthrone connector is deliberately absent: the shared function paragraph does not justify an exact enzyme or transformation here. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Emodin-anthrone oxidation (database annotation) has_named_product Emodin Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY6/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY6/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 73, sequence version 2. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:64268, left-to-right RHEA:64269; ECO:0000305 attributed to PMID:26242966. The atrochrysone-to-emodin-anthrone connector is deliberately absent: the shared function paragraph does not justify an exact enzyme or transformation here. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcA (Af293 reference) has_database_annotated_catalytic_role_in Emodin 8-O-methylation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQZ7/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89337.1
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ7/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 105, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:36643, left-to-right RHEA:36644; ECO:0000305 attributed to PMID:26242966. The annotation names the 8-hydroxy methylation, not any arbitrary emodin methylation. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    methylation of the 8-hydroxy group of emodin to form questin
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89337.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcA/AFUA_4G14580, 482 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: complement(join(AAHF01000005.1:77804..78152, AAHF01000005.1:78207..78474,AAHF01000005.1:78529..78972, AAHF01000005.1:79053..79440)). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
Emodin is_named_substrate_of Emodin 8-O-methylation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ7/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ7/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 105, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:36643, left-to-right RHEA:36644; ECO:0000305 attributed to PMID:26242966. The annotation names the 8-hydroxy methylation, not any arbitrary emodin methylation. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Emodin 8-O-methylation (database annotation) has_named_product Questin Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ7/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ7/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 105, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:36643, left-to-right RHEA:36644; ECO:0000305 attributed to PMID:26242966. The annotation names the 8-hydroxy methylation, not any arbitrary emodin methylation. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcG (Af293 reference) has_similarity_inferred_catalytic_role_in NADPH-dependent questin reduction (by similarity) Inferred by similarity, not directly demonstrated for this native sequence. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQZ1/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89343.1
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ1/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 87, sequence version 1. Inferred by similarity, not directly demonstrated for this native sequence. ECO:0000250 transfer from UniProtKB:Q0CCX9. RHEA:86147 is written in the oxidation direction; its annotated physiological direction is RIGHT-TO-LEFT, RHEA:86149. This graph correctly shows questin reduction with NADPH, not oxidation of the hydroquinone. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    reduction of the keto group at C-10 in questin
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89343.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcG/AFUA_4G14520, 263 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: complement(AAHF01000005.1:92193..92984). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
Questin is_named_substrate_of NADPH-dependent questin reduction (by similarity) Inferred by similarity, not directly demonstrated for this native sequence. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ1/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ1/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 87, sequence version 1. Inferred by similarity, not directly demonstrated for this native sequence. ECO:0000250 transfer from UniProtKB:Q0CCX9. RHEA:86147 is written in the oxidation direction; its annotated physiological direction is RIGHT-TO-LEFT, RHEA:86149. This graph correctly shows questin reduction with NADPH, not oxidation of the hydroquinone. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
NADPH-dependent questin reduction (by similarity) has_named_product Questin hydroquinone Inferred by similarity, not directly demonstrated for this native sequence. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ1/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ1/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 87, sequence version 1. Inferred by similarity, not directly demonstrated for this native sequence. ECO:0000250 transfer from UniProtKB:Q0CCX9. RHEA:86147 is written in the oxidation direction; its annotated physiological direction is RIGHT-TO-LEFT, RHEA:86149. This graph correctly shows questin reduction with NADPH, not oxidation of the hydroquinone. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcI (Af293 reference) has_similarity_inferred_catalytic_role_in Questin-hydroquinone ring cleavage (by similarity) Inferred by similarity, not directly demonstrated for this native sequence. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQY9/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89345.1
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY9/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 74, sequence version 1. Inferred by similarity, not directly demonstrated for this native sequence. Similarity-derived role: FUNCTION cites UniProtKB:Q0CCX8, while CATALYTIC ACTIVITY cites UniProtKB:Q0CCX5 (ECO:0000250). These are distinct transfer donors, not identified as the same protein. RHEA:86143, left-to-right RHEA:86144. The immediate substrate is the hydroquinone, not unreduced questin. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    cleavage of the C-10-C-4a bond of the questin hydroquinone intermediate
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89345.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcI/AFUA_4G14500, 445 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 1: Evidence at protein level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: complement(AAHF01000005.1:94755..96092). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
Questin hydroquinone is_named_substrate_of Questin-hydroquinone ring cleavage (by similarity) Inferred by similarity, not directly demonstrated for this native sequence. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY9/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY9/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 74, sequence version 1. Inferred by similarity, not directly demonstrated for this native sequence. Similarity-derived role: FUNCTION cites UniProtKB:Q0CCX8, while CATALYTIC ACTIVITY cites UniProtKB:Q0CCX5 (ECO:0000250). These are distinct transfer donors, not identified as the same protein. RHEA:86143, left-to-right RHEA:86144. The immediate substrate is the hydroquinone, not unreduced questin. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Questin-hydroquinone ring cleavage (by similarity) has_named_product Desmethylsulochrin Inferred by similarity, not directly demonstrated for this native sequence. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY9/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY9/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 74, sequence version 1. Inferred by similarity, not directly demonstrated for this native sequence. Similarity-derived role: FUNCTION cites UniProtKB:Q0CCX8, while CATALYTIC ACTIVITY cites UniProtKB:Q0CCX5 (ECO:0000250). These are distinct transfer donors, not identified as the same protein. RHEA:86143, left-to-right RHEA:86144. The immediate substrate is the hydroquinone, not unreduced questin. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcM (Af293 reference) has_database_annotated_catalytic_role_in Desmethylsulochrin methylation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQY5/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89349.1
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY5/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 86, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:84827, left-to-right RHEA:84828; ECO:0000305 attributed to PMID:26242966. The function uses desmethylsulochrin and the reaction uses demethylsulochrin. No independent exact intermediate structure or methylation-site grounding is added. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    methylation of desmethylsulochrin to yield sulochrin
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89349.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcM/AFUA_4G14460, 319 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: AAHF01000005.1:100642..101601. Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
Desmethylsulochrin is_named_substrate_of Desmethylsulochrin methylation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY5/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY5/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 86, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:84827, left-to-right RHEA:84828; ECO:0000305 attributed to PMID:26242966. The function uses desmethylsulochrin and the reaction uses demethylsulochrin. No independent exact intermediate structure or methylation-site grounding is added. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Desmethylsulochrin methylation (database annotation) has_named_product Sulochrin Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY5/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY5/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 86, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. RHEA:84827, left-to-right RHEA:84828; ECO:0000305 attributed to PMID:26242966. The function uses desmethylsulochrin and the reaction uses demethylsulochrin. No independent exact intermediate structure or methylation-site grounding is added. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcH (Af293 reference) has_database_annotated_catalytic_role_in Sulochrin methylation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQZ0/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89344.2
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ0/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 85, sequence version 2. Database-annotated role; primary catalytic experiment not independently inspected. Function annotation attributed to PMID:26242966. The database spelling monomethylsulfochrin is retained without assigning an exact chemical identifier; it is not silently normalized to monomethylsulochrin. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    methylation of sulochrin to yield monomethylsulfochrin
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89344.2
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcH/AFUA_4G14510, 282 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: join(AAHF01000005.1:93571..93937, AAHF01000005.1:94002..94040,AAHF01000005.1:94097..94153, AAHF01000005.1:94209..94594). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
Sulochrin is_named_substrate_of Sulochrin methylation (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ0/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ0/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 85, sequence version 2. Database-annotated role; primary catalytic experiment not independently inspected. Function annotation attributed to PMID:26242966. The database spelling monomethylsulfochrin is retained without assigning an exact chemical identifier; it is not silently normalized to monomethylsulochrin. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Sulochrin methylation (database annotation) has_named_product Monomethylsulfochrin (database spelling; identity unresolved) Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQZ0/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ0/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 85, sequence version 2. Database-annotated role; primary catalytic experiment not independently inspected. Function annotation attributed to PMID:26242966. The database spelling monomethylsulfochrin is retained without assigning an exact chemical identifier; it is not silently normalized to monomethylsulochrin. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcJ (Af293 reference) has_database_annotated_catalytic_role_in Terminal trypacidin-forming conversion (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Selected native Af293 reference, not a verified experimental allele.
https://www.uniprot.org/uniprotkb/Q4WQY8/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89346.1
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY8/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 111, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. Function annotation attributed to PMID:26242966. The database names a terminal conversion from monomethylsulfochrin to trypacidin; exact bond chemistry and stereochemical outcome are not independently established. The owner retains one unspecified stereocenter. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    conversion of monomethylsulfochrin to trypacidin
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89346.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcJ/AFUA_4G14490, 609 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: complement(join(AAHF01000005.1:96582..97879, AAHF01000005.1:97928..97941,AAHF01000005.1:97981..98072, AAHF01000005.1:98125..98249,AAHF01000005.1:98295..98342, AAHF01000005.1:98394..98463,AAHF01000005.1:98527..98709)). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
Monomethylsulfochrin (database spelling; identity unresolved) is_named_substrate_of Terminal trypacidin-forming conversion (database annotation) Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY8/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY8/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 111, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. Function annotation attributed to PMID:26242966. The database names a terminal conversion from monomethylsulfochrin to trypacidin; exact bond chemistry and stereochemical outcome are not independently established. The owner retains one unspecified stereocenter. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Terminal trypacidin-forming conversion (database annotation) has_named_product Trypacidin (stereochemistry unspecified) Database-annotated role; primary catalytic experiment not independently inspected. Named-intermediate view, not a balanced reaction or exact stereoisomer assignment.
https://www.uniprot.org/uniprotkb/Q4WQY8/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQY8/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 111, sequence version 1. Database-annotated role; primary catalytic experiment not independently inspected. Function annotation attributed to PMID:26242966. The database names a terminal conversion from monomethylsulfochrin to trypacidin; exact bond chemistry and stereochemical outcome are not independently established. The owner retains one unspecified stereocenter. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 7 nodes, 8 edges

Genetic and regulatory effects component. Across this owner's two components: Sequence-verified native Af293 references with explicitly database-level trypacidin roles, not verified experimental alleles. Ten enzyme annotations and two regulators are represented. TpcB/G/I catalytic roles remain similarity-derived. Named intermediates are not exact chemical-species groundings or balanced equations. No atrochrysone-to-emodin-anthrone connector is asserted, so this is not a complete reconstituted pathway. The database spelling monomethylsulfochrin and the product's unspecified stereochemistry remain unresolved. Genetic loss and impairment are distinguished; no direct promoter binding, physical complex, bioactivity target, virulence mechanism, universal essentiality or producer-grade upgrade is asserted. TpcF is held without a catalytic assignment.

SubjectPredicateObjectNotesEvidence
TpcD (Af293 reference) has_database_annotated_regulatory_role_in Database-reported trypacidin production contexts Native reference, not a verified experimental allele. Regulation does not establish direct promoter binding or a physical TpcD-TpcE complex.
https://www.uniprot.org/uniprotkb/Q4WQZ4/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89340.1
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ4/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 77, sequence version 1. TpcD is annotated as a coactivator and TpcE as a transcription factor regulating trypacidin production. Annotations cite PMID:26242966 and PMID:26278536; no particular promoter contact, complex stoichiometry or complete regulatory mechanism is asserted. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    With tpcE, coregulates the production of trypacidin
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89340.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcD/AFUA_4G14550, 437 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: join(AAHF01000005.1:87081..87830, AAHF01000005.1:87883..88446). Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
TpcE (Af293 reference) has_database_annotated_regulatory_role_in Database-reported trypacidin production contexts Native reference, not a verified experimental allele. Regulation does not establish direct promoter binding or a physical TpcD-TpcE complex.
https://www.uniprot.org/uniprotkb/Q4WQZ3/entry
https://www.ncbi.nlm.nih.gov/protein/EAL89341.2
https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ3/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 104, sequence version 2. TpcD is annotated as a coactivator and TpcE as a transcription factor regulating trypacidin production. Annotations cite PMID:26242966 and PMID:26278536; no particular promoter contact, complex stoichiometry or complete regulatory mechanism is asserted. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
    snippet
    Transcription factor that regulates the expression of the gene clusters
  • reference
    https://www.ncbi.nlm.nih.gov/protein/EAL89341.2
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Native Af293 NCBITaxon:330879 reference tpcE/AFUA_4G14540, 396 aa. Spliced contig and chromosome CDS sequences and translations, both deposited translations, separately retrieved GenPept and reviewed UniProt sequences agree exactly. Locus and explicit nucleotide/protein cross-references agree. Protein-existence annotation remains 2: Evidence at transcript level. This is not a verified experimental allele or an independent functional experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AAHF01000005.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Complete native CDS: AAHF01000005.1:89818..91008. Exon coordinates/strands, codon start 1, translation table 1 and unambiguous start/stop checked. AAHF01000005.1:77804..101601 maps exactly as reverse complement to CM000172.1:3822105..3845902 within the source BGC0001403 interval. This reference mapping does not upgrade the source producer or locus evidence grades.
TpcA (Af293 reference) has_database_annotated_deletion_associated_loss_of Database-reported trypacidin production contexts
https://www.uniprot.org/uniprotkb/Q4WQZ7/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ7/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB disruption phenotype annotation, release 2026_03, entry version 105, sequence version 1. The disruption annotation reports loss of trypacidin production. Database-cited PubMed sources: 26242966. Questin phenotypes and phagocytosis claims are not separately adopted here. Contexts from different papers are not equated; no universal essentiality claim. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcB (Af293 reference) has_database_annotated_deletion_associated_impairment_of Database-reported trypacidin production contexts
https://www.uniprot.org/uniprotkb/Q4WQZ6/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ6/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB disruption phenotype annotation, release 2026_03, entry version 85, sequence version 1. The disruption annotation reports impaired trypacidin production, not necessarily complete loss. Database-cited PubMed sources: 26242966, 26278536. Questin phenotypes and phagocytosis claims are not separately adopted here. Contexts from different papers are not equated; no universal essentiality claim. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcC (Af293 reference) has_database_annotated_deletion_associated_impairment_of Database-reported trypacidin production contexts
https://www.uniprot.org/uniprotkb/Q4WQZ5/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ5/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB disruption phenotype annotation, release 2026_03, entry version 119, sequence version 1. The disruption annotation reports impaired trypacidin production, not necessarily complete loss. Database-cited PubMed sources: 26242966, 26278536, 27611015. Questin phenotypes and phagocytosis claims are not separately adopted here. Contexts from different papers are not equated; no universal essentiality claim. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcD (Af293 reference) has_database_annotated_deletion_associated_loss_of Database-reported trypacidin production contexts
https://www.uniprot.org/uniprotkb/Q4WQZ4/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ4/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB disruption phenotype annotation, release 2026_03, entry version 77, sequence version 1. The disruption annotation reports loss of trypacidin production. Database-cited PubMed sources: 26242966, 26278536. Questin phenotypes and phagocytosis claims are not separately adopted here. Contexts from different papers are not equated; no universal essentiality claim. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
TpcE (Af293 reference) has_database_annotated_deletion_associated_loss_of Database-reported trypacidin production contexts
https://www.uniprot.org/uniprotkb/Q4WQZ3/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ3/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB disruption phenotype annotation, release 2026_03, entry version 104, sequence version 2. The disruption annotation reports loss of trypacidin production. Database-cited PubMed sources: 26242966. Questin phenotypes and phagocytosis claims are not separately adopted here. Contexts from different papers are not equated; no universal essentiality claim. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.
Database-reported trypacidin production contexts has_named_product Trypacidin (stereochemistry unspecified)
https://www.uniprot.org/uniprotkb/Q4WQZ3/entry
  • reference
    https://www.uniprot.org/uniprotkb/Q4WQZ3/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Reviewed UniProtKB function annotation, release 2026_03, entry version 104, sequence version 2. The annotation names trypacidin. This product-context edge is not a terminal catalytic reaction or proof of production in every mutant. The unchanged owner representation matches PubChem CID3035292 and RDKit, with one unspecified stereocenter; an exact natural stereoisomer is not established. Source annotations are not newly inspected primary experiments; native reference identity does not establish the assayed strain or construct sequence.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (trypacidin) ↗ MediaIngredientMech (naturalproductmech:mibig-cfc470b905) ↗ TaxonMech: Aspergillus fumigatus Af293 (NCBITaxon:330879) ↗ PathwayMech (mibig:BGC0001403) ↗

Open questions 1

curator-trypacidin-reference-and-pathway-limits — What is TpcF's function, and which primary experiments resolve the missing connector, intermediate identities and experimental alleles?

Provenance

Source conceptSourceVersion
BGC0001403MIBIG3

This page is generated from data/natural_products/polyketides/trypacidin.yaml, which is generated from the committed inventories. Neither is edited by hand.