NaturalProductMech

Browse · Polyketides · youssoufene A1

youssoufene A1

naturalproductmech:mibig-78ea4c55fb MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.
What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKeyPBGNPVWDTRYFBH-GSOXOQHNSA-N
SMILESC/C=C/C=C\C=C\C=C/C1=CC=CC=C1[C@@H](CC(=O)O)[C@@H]1C=C/C(=C\CC(=O)O)[C@@H](/C=C\C=C\C=C/C=C/C)C1
Stereochemistryfully defined
Cross-referencesnpatlas:NPA032222

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 2

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Streptomyces youssoufiensis
Strain: OUC6819
NCBITaxon:654447		 BGC_CHARACTERIZED mibig:BGC0002547
PMID:31891272
  • reference
    PMID:31891272
    evidence type
    DATABASE_ASSERTION
    notes
    MIBiG BGC0002547 entry version 2; citation taken from the entry level
  • reference
    PMID:31891272
    evidence type
    CURATOR_INFERENCE
    notes
    Strain context: OUC6819. Li et al. 2020 (PMID:31891272, DOI:10.1021/acs.orglett.9b04559), Liu et al. 2021 (PMID:33155752, DOI:10.1002/anie.202007777), and GenBank MN844187.1 explicitly identify Streptomyces youssoufiensis strain OUC6819 as the sequenced and genetically characterized ysf cluster host for BGC0002547:1 (youssoufene A1). Preserve NCBITaxon:654447 and existing BGC_CHARACTERIZED/CLUSTER_DEMONSTRATED grades; separate BGC0002510 deposit metadata remains tracked in #1046. See issue #1044. Taxon identity and evidence grade are unchanged; this is not a species-wide experiment. MIBiG source row BGC0002547:1; SHA256 1d48b2327fd1ff6df8dacc84f722e6dcef3c298cafc6442b51a0d2190b11547f. Curator: antigravity; date: 2026-10-30.
Streptomyces sp.
NCBITaxon:1931		 SOURCE_ASSERTION mibig:BGC0002510 PMID:32860295

Occurrences 1

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Streptomyces youssoufiensis
NCBITaxon:654447		 LOTUS DOI:10.1021/ACS.ORGLETT.9B04559

Biosynthetic gene clusters 2

AccessionHostLocus evidenceGenome
mibig:BGC0002547 Streptomyces youssoufiensis
NCBITaxon:654447 		CLUSTER_DEMONSTRATED genbank:MN844187.1
mibig:BGC0002510 Streptomyces sp.
NCBITaxon:1931 		CLUSTER_DEMONSTRATED genbank:MT459140.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Biosynthesis curated

Written by a curator with claim-level evidence on each step; not derived from a source. This is what the rest of the record exists to support.

#EnzymeReactionSubstrate → productEvidence
1 YsfB/YsfC KS/CLF complex ungrounded → ungrounded DOI:10.1002/anie.202007777
2 YsfD/YsfE KS/CLF complex ungrounded → ungrounded DOI:10.1002/anie.202007777
3 YsfG isomerase
NCBIProtein:QHB92594.1		 ungrounded → ungrounded DOI:10.1002/anie.202007777
4 YsfJ/YsfK KS/CLF complex ungrounded → ungrounded DOI:10.1002/anie.202007777

Entry 1: This curates YsfB/QHB92589.1 and YsfC/QHB92590.1 as one of three OUC6819 type II ketosynthase/chain-length-factor complexes assigned to cinnamoyl-lipid skeleton assembly. The exact isolated substrate/product pair is unresolved, so enzyme_id is left unfilled while official NCBI accessions ground the subunits.

Entry 2: This curates YsfD/QHB92591.1 and YsfE/QHB92592.1 as a second OUC6819 type II ketosynthase/chain-length-factor complex assigned to modular youssoufene skeleton assembly. The exact isolated substrate/product pair is unresolved, so enzyme_id is left unfilled while official NCBI accessions ground the subunits.

Entry 3: This curates YsfG/QHB92594.1 as the OUC6819 isomerase in the isomerase-dependent ACP-tethered chain-elongation process. The exact free substrate and product are not represented, while enzyme_id is grounded to the native GenPept accession NCBIProtein:QHB92594.1 (UniParc UPI001320438E maps only to retired/obsolete UniProtKB/TrEMBL entry A0A6B9MI90.1).

Entry 4: This curates YsfJ/QHB92597.1 and YsfK/QHB92598.1 as the third OUC6819 type II ketosynthase/chain-length-factor complex assigned to modular youssoufene skeleton assembly. The exact isolated substrate/product pair is unresolved, so enzyme_id is left unfilled while official NCBI accessions ground the subunits.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 2 producers (1 causal) 1 cited occurrence BIOSYNTHETIC LOCUS 2 gene clusters 4 curated pathway steps NATURAL PRODUCT youssoufene A1 naturalproductmech:mibig-78ea4c55fb Polyketides TARGETS & BIOACTIVITY 0 molecular targets 0 bioactivities · 1 graph

Causal graph — biosynthesis 13 nodes, 12 edges

Partial Streptomyces youssoufiensis OUC6819 Ysf type II PKS graph for the three KS/CLF complexes and isomerase-dependent ACP-tethered polyunsaturated chain elongation that contribute to the youssoufene A1 skeleton.

SubjectPredicateObjectNotesEvidence
YsfB 3-oxoacyl-ACP synthase is subunit of YsfB/YsfC KS/CLF complex YsfB and YsfC were resolved as an OUC6819 KS/CLF pair.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92589.1
https://www.uniprot.org/uniparc/UPI00131FE52F
https://www.ncbi.nlm.nih.gov/protein/QHB92590.1
https://www.uniprot.org/uniparc/UPI00131FCA94
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92589.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfB [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92589.1 is the Streptomyces youssoufiensis OUC6819 ysfB coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FE52F
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92589.1
    notes
    UniParc UPI00131FE52F archives the exact 403 aa Streptomyces youssoufiensis OUC6819 YsfB sequence (EMBL MN844187.1 protein QHB92589.1, gene ysfB) and records that UniProtKB/TrEMBL entry A0A6B9MIZ5.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92589.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92590.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfC [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92590.1 is the Streptomyces youssoufiensis OUC6819 ysfC coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FCA94
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92590.1
    notes
    UniParc UPI00131FCA94 archives the exact 390 aa Streptomyces youssoufiensis OUC6819 YsfC sequence (EMBL MN844187.1 protein QHB92590.1, gene ysfC) and records that UniProtKB/TrEMBL entry A0A6B9MG83.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92590.1.
YsfC 3-oxoacyl-ACP synthase is subunit of YsfB/YsfC KS/CLF complex YsfB and YsfC were resolved as an OUC6819 KS/CLF pair.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92589.1
https://www.uniprot.org/uniparc/UPI00131FE52F
https://www.ncbi.nlm.nih.gov/protein/QHB92590.1
https://www.uniprot.org/uniparc/UPI00131FCA94
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92589.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfB [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92589.1 is the Streptomyces youssoufiensis OUC6819 ysfB coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FE52F
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92589.1
    notes
    UniParc UPI00131FE52F archives the exact 403 aa Streptomyces youssoufiensis OUC6819 YsfB sequence (EMBL MN844187.1 protein QHB92589.1, gene ysfB) and records that UniProtKB/TrEMBL entry A0A6B9MIZ5.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92589.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92590.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfC [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92590.1 is the Streptomyces youssoufiensis OUC6819 ysfC coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FCA94
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92590.1
    notes
    UniParc UPI00131FCA94 archives the exact 390 aa Streptomyces youssoufiensis OUC6819 YsfC sequence (EMBL MN844187.1 protein QHB92590.1, gene ysfC) and records that UniProtKB/TrEMBL entry A0A6B9MG83.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92590.1.
YsfD beta-ketoacyl synthase is subunit of YsfD/YsfE KS/CLF complex YsfD and YsfE were resolved as an OUC6819 KS/CLF pair.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92591.1
https://www.uniprot.org/uniparc/UPI00131FC4C1
https://www.ncbi.nlm.nih.gov/protein/QHB92592.1
https://www.uniprot.org/uniparc/UPI00131FAF34
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92591.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfD [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92591.1 is the Streptomyces youssoufiensis OUC6819 ysfD coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FC4C1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92591.1
    notes
    UniParc UPI00131FC4C1 archives the exact 403 aa Streptomyces youssoufiensis OUC6819 YsfD sequence (EMBL MN844187.1 protein QHB92591.1, gene ysfD) and records that UniProtKB/TrEMBL entry A0A6B9MDK8.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92591.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92592.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfE [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92592.1 is the Streptomyces youssoufiensis OUC6819 ysfE coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FAF34
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92592.1
    notes
    UniParc UPI00131FAF34 archives the exact 304 aa Streptomyces youssoufiensis OUC6819 YsfE sequence (EMBL MN844187.1 protein QHB92592.1, gene ysfE) and records that UniProtKB/TrEMBL entry A0A6B9MQI2.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92592.1.
YsfE beta-ketoacyl synthase is subunit of YsfD/YsfE KS/CLF complex YsfD and YsfE were resolved as an OUC6819 KS/CLF pair.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92591.1
https://www.uniprot.org/uniparc/UPI00131FC4C1
https://www.ncbi.nlm.nih.gov/protein/QHB92592.1
https://www.uniprot.org/uniparc/UPI00131FAF34
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92591.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfD [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92591.1 is the Streptomyces youssoufiensis OUC6819 ysfD coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FC4C1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92591.1
    notes
    UniParc UPI00131FC4C1 archives the exact 403 aa Streptomyces youssoufiensis OUC6819 YsfD sequence (EMBL MN844187.1 protein QHB92591.1, gene ysfD) and records that UniProtKB/TrEMBL entry A0A6B9MDK8.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92591.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92592.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfE [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92592.1 is the Streptomyces youssoufiensis OUC6819 ysfE coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FAF34
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92592.1
    notes
    UniParc UPI00131FAF34 archives the exact 304 aa Streptomyces youssoufiensis OUC6819 YsfE sequence (EMBL MN844187.1 protein QHB92592.1, gene ysfE) and records that UniProtKB/TrEMBL entry A0A6B9MQI2.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92592.1.
YsfJ 3-oxoacyl-ACP synthase is subunit of YsfJ/YsfK KS/CLF complex YsfJ and YsfK were resolved as an OUC6819 KS/CLF pair.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92597.1
https://www.uniprot.org/uniparc/UPI00131F724F
https://www.ncbi.nlm.nih.gov/protein/QHB92598.1
https://www.uniprot.org/uniparc/UPI0013202848
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92597.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfJ [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92597.1 is the Streptomyces youssoufiensis OUC6819 ysfJ coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131F724F
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92597.1
    notes
    UniParc UPI00131F724F archives the exact 427 aa Streptomyces youssoufiensis OUC6819 YsfJ sequence (EMBL MN844187.1 protein QHB92597.1, gene ysfJ) and records that UniProtKB/TrEMBL entry A0A6B9MQD9.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92597.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92598.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfK [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92598.1 is the Streptomyces youssoufiensis OUC6819 ysfK coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI0013202848
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92598.1
    notes
    UniParc UPI0013202848 archives the exact 353 aa Streptomyces youssoufiensis OUC6819 YsfK sequence (EMBL MN844187.1 protein QHB92598.1, gene ysfK) and records that UniProtKB/TrEMBL entry A0A6B9MDM2.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92598.1.
YsfK 3-oxoacyl-ACP synthase is subunit of YsfJ/YsfK KS/CLF complex YsfJ and YsfK were resolved as an OUC6819 KS/CLF pair.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92597.1
https://www.uniprot.org/uniparc/UPI00131F724F
https://www.ncbi.nlm.nih.gov/protein/QHB92598.1
https://www.uniprot.org/uniparc/UPI0013202848
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92597.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfJ [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92597.1 is the Streptomyces youssoufiensis OUC6819 ysfJ coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131F724F
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92597.1
    notes
    UniParc UPI00131F724F archives the exact 427 aa Streptomyces youssoufiensis OUC6819 YsfJ sequence (EMBL MN844187.1 protein QHB92597.1, gene ysfJ) and records that UniProtKB/TrEMBL entry A0A6B9MQD9.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92597.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92598.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfK [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92598.1 is the Streptomyces youssoufiensis OUC6819 ysfK coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI0013202848
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92598.1
    notes
    UniParc UPI0013202848 archives the exact 353 aa Streptomyces youssoufiensis OUC6819 YsfK sequence (EMBL MN844187.1 protein QHB92598.1, gene ysfK) and records that UniProtKB/TrEMBL entry A0A6B9MDM2.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92598.1.
YsfB/YsfC KS/CLF complex modularly assembles ACP-tethered polyunsaturated chain elongation The YsfB/YsfC edge is kept at the chain-elongation process level because the curated claim is to one of three modular KS/CLF complexes, not an isolated conversion.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92589.1
https://www.uniprot.org/uniparc/UPI00131FE52F
https://www.ncbi.nlm.nih.gov/protein/QHB92590.1
https://www.uniprot.org/uniparc/UPI00131FCA94
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92589.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfB [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92589.1 is the Streptomyces youssoufiensis OUC6819 ysfB coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FE52F
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92589.1
    notes
    UniParc UPI00131FE52F archives the exact 403 aa Streptomyces youssoufiensis OUC6819 YsfB sequence (EMBL MN844187.1 protein QHB92589.1, gene ysfB) and records that UniProtKB/TrEMBL entry A0A6B9MIZ5.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92589.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92590.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfC [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92590.1 is the Streptomyces youssoufiensis OUC6819 ysfC coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FCA94
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92590.1
    notes
    UniParc UPI00131FCA94 archives the exact 390 aa Streptomyces youssoufiensis OUC6819 YsfC sequence (EMBL MN844187.1 protein QHB92590.1, gene ysfC) and records that UniProtKB/TrEMBL entry A0A6B9MG83.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92590.1.
YsfD/YsfE KS/CLF complex modularly assembles ACP-tethered polyunsaturated chain elongation The YsfD/YsfE edge is kept at the chain-elongation process level because the curated claim is to one of three modular KS/CLF complexes, not an isolated conversion.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92591.1
https://www.uniprot.org/uniparc/UPI00131FC4C1
https://www.ncbi.nlm.nih.gov/protein/QHB92592.1
https://www.uniprot.org/uniparc/UPI00131FAF34
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92591.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfD [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92591.1 is the Streptomyces youssoufiensis OUC6819 ysfD coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FC4C1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92591.1
    notes
    UniParc UPI00131FC4C1 archives the exact 403 aa Streptomyces youssoufiensis OUC6819 YsfD sequence (EMBL MN844187.1 protein QHB92591.1, gene ysfD) and records that UniProtKB/TrEMBL entry A0A6B9MDK8.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92591.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92592.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfE [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92592.1 is the Streptomyces youssoufiensis OUC6819 ysfE coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131FAF34
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92592.1
    notes
    UniParc UPI00131FAF34 archives the exact 304 aa Streptomyces youssoufiensis OUC6819 YsfE sequence (EMBL MN844187.1 protein QHB92592.1, gene ysfE) and records that UniProtKB/TrEMBL entry A0A6B9MQI2.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92592.1.
YsfJ/YsfK KS/CLF complex modularly assembles ACP-tethered polyunsaturated chain elongation The YsfJ/YsfK edge is kept at the chain-elongation process level because the curated claim is to one of three modular KS/CLF complexes, not an isolated conversion.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92597.1
https://www.uniprot.org/uniparc/UPI00131F724F
https://www.ncbi.nlm.nih.gov/protein/QHB92598.1
https://www.uniprot.org/uniparc/UPI0013202848
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92597.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfJ [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92597.1 is the Streptomyces youssoufiensis OUC6819 ysfJ coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI00131F724F
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92597.1
    notes
    UniParc UPI00131F724F archives the exact 427 aa Streptomyces youssoufiensis OUC6819 YsfJ sequence (EMBL MN844187.1 protein QHB92597.1, gene ysfJ) and records that UniProtKB/TrEMBL entry A0A6B9MQD9.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92597.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92598.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfK [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92598.1 is the Streptomyces youssoufiensis OUC6819 ysfK coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI0013202848
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92598.1
    notes
    UniParc UPI0013202848 archives the exact 353 aa Streptomyces youssoufiensis OUC6819 YsfK sequence (EMBL MN844187.1 protein QHB92598.1, gene ysfK) and records that UniProtKB/TrEMBL entry A0A6B9MDM2.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92598.1.
YsfG isomerase is required for ACP-tethered polyunsaturated chain elongation YsfG is represented as a required isomerase in the ACP-tethered elongation process without asserting a specific free substrate/product pair.
DOI:10.1002/anie.202007777
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
https://www.ncbi.nlm.nih.gov/protein/QHB92594.1
https://www.uniprot.org/uniparc/UPI001320438E
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    isomerase-dependent ACP-tethered polyunsaturated chain elongation process
    notes
    Liu et al. used the native Ysf system to connect the OUC6819 isomerase step with the ACP-tethered polyunsaturated-chain elongation process in youssoufene biosynthesis.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QHB92594.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    YsfG [Streptomyces youssoufiensis]
    notes
    NCBI Protein QHB92594.1 is the Streptomyces youssoufiensis OUC6819 ysfG coding sequence from the MN844187.1 youssoufenes biosynthetic gene cluster. Exact GenPept accession and gene-name searches did not resolve this protein in UniProtKB on 2026-09-27.
  • reference
    https://www.uniprot.org/uniparc/UPI001320438E
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-23
    snippet
    QHB92594.1
    notes
    UniParc UPI001320438E archives the exact 213 aa Streptomyces youssoufiensis OUC6819 YsfG sequence (EMBL MN844187.1 protein QHB92594.1, gene ysfG) and records that UniProtKB/TrEMBL entry A0A6B9MI90.1 is inactive/obsolete, confirming no active UniProtKB accession resolves for QHB92594.1.
ACP-tethered polyunsaturated chain elongation precedes benzene ring closure The edge captures the source ordering that benzene-ring closure follows ACP-tethered C18 polyene formation.
DOI:10.1002/anie.202007777
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    benzene ring closure happens right after the formation
    notes
    Liu et al. placed benzene-ring closure immediately after formation of the ACP-tethered C18 polyene in the OUC6819 Ysf type II PKS system.
benzene ring closure contributes to youssoufene A1 The Ysf KS/CLF graph contributes to the youssoufene skeleton shared by dimeric A1 and monomeric B1-B4 congeners; exact A1 dimerization and release are left unexpanded.
DOI:10.1002/anie.202007777
DOI:10.1021/acs.orglett.9b04559
https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
  • reference
    DOI:10.1002/anie.202007777
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    YsfB/C, YsfD/E, and YsfJ/K
    notes
    Liu et al. used in vivo gene inactivation, in vitro reconstitution, and intracellular tagged-carrier-protein tracking to assign the Streptomyces youssoufiensis OUC6819 YsfB/YsfC, YsfD/YsfE, and YsfJ/YsfK ketosynthase/chain-length-factor complexes to modular assembly of the youssoufene skeleton.
  • reference
    DOI:10.1021/acs.orglett.9b04559
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    activated the production of a novel dimeric benzoic polyene acids
    notes
    Li et al. showed that disruption of dtlA in marine-derived Streptomyces youssoufiensis OUC6819 activated youssoufene A1 and the monomeric youssoufenes B1-B4.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0002547.2/index.html
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    notes
    MIBiG BGC0002547.2 names youssoufene A1 as a product of the Streptomyces youssoufiensis OUC6819 MN844187.1 locus and records knock-out support for the cluster.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MN844187.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-09-27
    snippet
    youssoufenes biosynthetic gene cluster
    notes
    GenBank MN844187.1 is the 20,186 bp Streptomyces youssoufiensis strain OUC6819 youssoufenes biosynthetic gene cluster and carries ysfB/QHB92589.1, ysfC/QHB92590.1, ysfD/QHB92591.1, ysfE/QHB92592.1, ysfG/QHB92594.1, ysfJ/QHB92597.1, and ysfK/QHB92598.1.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (youssoufene A1) ↗ MediaIngredientMech (naturalproductmech:mibig-78ea4c55fb) ↗ TaxonMech: Streptomyces youssoufiensis (NCBITaxon:654447) ↗ TaxonMech: Streptomyces sp. (NCBITaxon:1931) ↗ PathwayMech (mibig:BGC0002547) ↗ PathwayMech (mibig:BGC0002510) ↗

Provenance

Source conceptSourceVersion
BGC0002547MIBIG2
BGC0002510MIBIG2

This page is generated from data/natural_products/polyketides/youssoufene-a1.yaml, which is generated from the committed inventories. Neither is edited by hand.