NaturalProductMech

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2-methylisoborneol

naturalproductmech:mibig-0eb8c93c6f MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.
What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKeyLFYXNXGVLGKVCJ-UHFFFAOYSA-N
SMILESCC1(C2CCC1(C(C2)(C)O)C)C
Stereochemistry undefined stereocentres — the InChIKey does not distinguish this compound from its stereoisomers
Cross-referencespubchem:16913

Filing pathway

Terpenoids — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is terpene, from MIBiG.

Producer organisms 2

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Streptomyces griseus subsp. griseus NBRC 13350
NCBITaxon:455632		 SOURCE_ASSERTION mibig:BGC0000658 PMID:17369815
Saccharopolyspora erythraea NRRL 2338
NCBITaxon:405948		 SOURCE_ASSERTION mibig:BGC0000659 PMID:17369815

Occurrences 13

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Saccharopolyspora erythraea
NCBITaxon:1836		 LOTUS DOI:10.1038/NBT1297
Streptomyces albidoflavus
NCBITaxon:1886		 LOTUS DOI:10.1007/S10295-013-1383-2
Streptomyces albidoflavus
NCBITaxon:1886		 LOTUS DOI:10.1021/BI201827A
Streptomyces albidoflavus
NCBITaxon:1886		 LOTUS DOI:10.1021/JA803639G
Streptomyces coelicolor
NCBITaxon:1902		 LOTUS DOI:10.1007/S10295-013-1383-2
Streptomyces coelicolor
NCBITaxon:1902		 LOTUS DOI:10.1021/BI201827A
Streptomyces coelicolor
NCBITaxon:1902		 LOTUS DOI:10.1021/JA803639G
Phormidium tenue
NCBITaxon:426436		 LOTUS DOI:10.1248/CPB.31.659
Streptomyces collinus
NCBITaxon:42684		 LOTUS DOI:10.1007/S10295-015-1685-7
Streptomyces alboflavus
NCBITaxon:67267		 LOTUS DOI:10.1007/S12275-013-2586-Y
Streptomyces alboflavus
NCBITaxon:67267		 LOTUS DOI:10.1111/1574-6968.12088
Streptomyces spectabilis
NCBITaxon:68270		 LOTUS DOI:10.1007/S10295-019-02172-8
Streptomyces violaceusniger
NCBITaxon:68280		 LOTUS DOI:10.1039/C4CC00177J

Biosynthetic gene clusters 2

AccessionHostLocus evidenceGenome
mibig:BGC0000658 Streptomyces griseus subsp. griseus NBRC 13350
NCBITaxon:455632 		CLUSTER_UNSTATED genbank:AP009493.1
mibig:BGC0000659 Saccharopolyspora erythraea NRRL 2338
NCBITaxon:405948 		CLUSTER_UNSTATED genbank:AM420293.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Biosynthesis curated

Written by a curator with claim-level evidence on each step; not derived from a source. This is what the rest of the record exists to support.

#EnzymeReactionSubstrate → productEvidence
1 SGR_1268 (geranyl diphosphate 2-C-methyltransferase; proposed)
UniProtKB:B1VVB3		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/B1VVB3/entry
2 SGR_1269 (2-methylisoborneol synthase; proposed)
UniProtKB:B1VVB4		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/B1VVB4/entry
3 SACE_3721 (geranyl diphosphate 2-C-methyltransferase; proposed)
UniProtKB:A4FG18		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/A4FG18/entry
4 SACE_3722 (2-methylisoborneol synthase; proposed)
UniProtKB:A4FG19		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/A4FG19/entry
5 SAML0357 (2-methylisoborneol synthase; proposed)
UniProtKB:A3KI17		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/A3KI17/entry
6 SAML0358 (geranyl diphosphate 2-C-methyltransferase; proposed)
UniProtKB:A3KI18		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/A3KI18/entry
7 ScMIBS (2-methylisoborneol synthase; canonical reference)
UniProtKB:Q9F1Y6		 ungrounded → ungrounded DOI:10.1002/chem.202300775
8 SeMIBS (2-methylisoborneol synthase; canonical reference)
UniProtKB:K0K6P8		 ungrounded → ungrounded DOI:10.1002/chem.202300775
9 SpMIBS (2-methylisoborneol synthase; canonical reference)
NCBIProtein:WP_019890277.1		 ungrounded → ungrounded DOI:10.1002/chem.202300775
10 SCO7701 (geranyl diphosphate 2-C-methyltransferase; database-supported)
UniProtKB:Q9F1Y5		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/Q9F1Y5/entry
11 Strain-13189 2-methylisoborneol synthase (similarity-inferred)
UniProtKB:Q9F1V8		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/Q9F1V8/entry
12 Tpc (2-methylisoborneol synthase; database-supported)
UniProtKB:D3KYU2		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/D3KYU2/entry
13 Gdpmt (geranyl diphosphate 2-C-methyltransferase; database-supported)
UniProtKB:D3KYU3		 ungrounded → ungrounded https://www.uniprot.org/uniprotkb/D3KYU3/entry

Entry 1: SGR_1268/BAG18097.1, 292 aa. Native-reference assignment in Streptomyces griseus subsp. griseus NBRC 13350 (NCBITaxon:455632), not an identified assayed construct. UniProt automated function and the adopted MIBiG BGC0000658 v3 locus support a proposed biosynthetic role. No new primary experiment, exact reaction, stereochemical assignment, pathway order, shell-cargo relationship or producer/locus evidence upgrade is asserted.

Entry 2: SGR_1269/BAG18098.1, 437 aa. Native-reference assignment in Streptomyces griseus subsp. griseus NBRC 13350 (NCBITaxon:455632), not an identified assayed construct. UniProt automated function and the adopted MIBiG BGC0000658 v3 locus support a proposed biosynthetic role. No new primary experiment, exact reaction, stereochemical assignment, pathway order, shell-cargo relationship or producer/locus evidence upgrade is asserted.

Entry 3: SACE_3721/CAM02993.1, 285 aa. Native-reference assignment in Saccharopolyspora erythraea NRRL 2338 (NCBITaxon:405948). The UniProt functional annotation is curator inference (ECO:0000305), not an isolated-enzyme assay for this sequence. The adopted MIBiG BGC0000659 v3 locus supports the proposed compound association. No exact assayed construct, reaction, pathway order, stereochemical assignment, shell-cargo relationship or producer/locus evidence upgrade is asserted.

Entry 4: SACE_3722/CAM02994.1, 354 aa. Native-reference assignment in Saccharopolyspora erythraea NRRL 2338 (NCBITaxon:405948). The UniProt functional annotation is curator inference (ECO:0000305), not an isolated-enzyme assay for this sequence. The adopted MIBiG BGC0000659 v3 locus supports the proposed compound association. No exact assayed construct, reaction, pathway order, stereochemical assignment, shell-cargo relationship or producer/locus evidence upgrade is asserted.

Entry 5: SAML0357/SAM23877_0409, 440 aa. Native-reference protein in Streptomyces ambofaciens ATCC 23877 (NCBITaxon:278992), not an identified assayed construct. UniProt assigns function by curator inference (ECO:0000305) and explicitly links this entry to 2-methylisoborneol biosynthesis. This proposed role does not assert an exact reaction, pathway order, stereochemistry, shell-cargo relationship or new producer/BGC evidence grade.

Entry 6: SAML0358/SAM23877_0410, 291 aa. Native-reference protein in Streptomyces ambofaciens ATCC 23877 (NCBITaxon:278992), not an identified assayed construct. UniProt assigns function by curator inference (ECO:0000305) and explicitly links this entry to 2-methylisoborneol biosynthesis. This proposed role does not assert an exact reaction, pathway order, stereochemistry, shell-cargo relationship or new producer/BGC evidence grade.

Entry 7: ScMIBS is grounded to the exact 440-aa SCO7700 reference, not a truncated domain or variant. Canonical-reference mapping, not exact cloned-insert/tag/linker reconstruction. This is a separate coupled in-vitro assay, not a shared native pathway. No reaction order, native producer/BGC grade, kinetic value or stereochemical upgrade is asserted.

Entry 8: SeMIBS is grounded to the exact 362-aa BN6_59350 reference; unreviewed status does not replace the independently cited experiment. Canonical-reference mapping, not exact cloned-insert/tag/linker reconstruction. This is a separate coupled in-vitro assay, not a shared native pathway. No reaction order, native producer/BGC grade, kinetic value or stereochemical upgrade is asserted.

Entry 9: SpMIBS uses the exact 438-aa RefSeq reference. A0A918LM63 differs at positions 107 (T/A) and 116 (G/R), so it is not substituted as an exact UniProt mapping. Canonical-reference mapping, not exact cloned-insert/tag/linker reconstruction. This is a separate coupled in-vitro assay, not a shared native pathway. No reaction order, native producer/BGC grade, kinetic value or stereochemical upgrade is asserted.

Entry 10: SCO7701/Q9F1Y5, 292 aa, is the native canonical reference. The non-proposed assignment reflects UniProt experimental attribution, not a newly inspected primary assay. The database assigns GPP methylation to 2-MeGPP in methylisoborneol biosynthesis; this edge does not assert that the methyltransferase alone produces 2-MIB. Genomic A3(2), 1147, the UniProt M145 label and the cited NRRL B-16638 stock are not equated. No exact construct, reaction order, kinetics, shell-cargo relationship, native producer/BGC grade or stereochemical upgrade is asserted.

Entry 11: Q9F1V8/BAB20507.1, 437 aa, is a native strain-13189 reference with a similarity-inferred MIB synthase role, not an assayed protein. Do not identify strain 13189 with NBRC 13350 or borrow BGC0000658. The adjacent putative methyltransferase BAB20508.1 is not assigned a specific MIB function from neighborhood alone. No exact construct, reaction order, kinetics, shell-cargo relationship, native producer/BGC grade or stereochemical upgrade is asserted.

Entry 12: D3KYU2/BAI77523.1/tpc, 481 aa, is a deposited ATCC 31180 reference from Streptomyces lasalocidi (literature name S. lasaliensis), not an established native-locus or exact assay-construct assignment. The non-proposed role reflects UniProt experimental attribution, not a newly inspected primary assay. Use the explicit ATCC 31180 designation; uncertain DSM aliases are not equated. No reaction order, kinetic value, native production, producer/BGC grade, shell-cargo relationship or stereochemical resolution is asserted.

Entry 13: D3KYU3/BAI77524.1/gdpmt, 300 aa, is a deposited ATCC 31180 reference from Streptomyces lasalocidi (literature name S. lasaliensis), not an established native-locus or exact assay-construct assignment. The non-proposed role reflects UniProt experimental attribution, not a newly inspected primary assay. Use the explicit ATCC 31180 designation; uncertain DSM aliases are not equated. No reaction order, kinetic value, native production, producer/BGC grade, shell-cargo relationship or stereochemical resolution is asserted. The methyltransferase role does not assert that this enzyme alone produces 2-MIB.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 2 producers (0 causal) 13 cited occurrences BIOSYNTHETIC LOCUS 2 gene clusters 13 curated pathway steps NATURAL PRODUCT 2-methylisoborneol naturalproductmech:mibig-0eb8c93c6f Terpenoids TARGETS & BIOACTIVITY 0 molecular targets 0 bioactivities · 7 graphs

Causal graph — biosynthesis 3 nodes, 2 edges

Two proposed enzyme roles for the adopted NBRC 13350 locus. Native-reference protein identities are grounded; functional evidence is automated, not an assayed-sequence claim. The owner retains its unspecified stereochemistry. SGR_1270/SgEnc and the cyanobacterial same-name record with different connectivity are not mapped here.

SubjectPredicateObjectNotesEvidence
SGR_1268 (geranyl diphosphate 2-C-methyltransferase; proposed) is_database_proposed_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) SGR_1268/BAG18097.1, 292 aa. Native-reference assignment in Streptomyces griseus subsp. griseus NBRC 13350 (NCBITaxon:455632), not an identified assayed construct. UniProt automated function and the adopted MIBiG BGC0000658 v3 locus support a proposed biosynthetic role. No new primary experiment, exact reaction, stereochemical assignment, pathway order, shell-cargo relationship or producer/locus evidence upgrade is asserted.
https://www.uniprot.org/uniprotkb/B1VVB3/entry
https://www.ncbi.nlm.nih.gov/protein/BAG18097.1
https://mibig.secondarymetabolites.org/repository/BGC0000658/
  • reference
    https://www.uniprot.org/uniprotkb/B1VVB3/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    Catalyzes the SAM-dependent methylation of geranyl diphosphate (GPP) to yield (E)-2-methylgeranyl diphosphate (2-MeGPP)
    retrieved on
    2026-10-08
    notes
    UniProtKB release 2026_03, unreviewed entry 74, sequence 1. Automated ECO:0000256 annotation, not direct experimental evidence for this sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/BAG18097.1
    evidence type
    DATABASE_ASSERTION
    snippet
    SGR_1268
    retrieved on
    2026-10-09
    notes
    Native NBRC 13350 reference, 292 aa, reverse-strand AP009493.1:1490671..1491549. GenPept, UniProt, annotated CDS and table-11 CDS translation agree exactly. Identity evidence only.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000658/
    evidence type
    CURATOR_INFERENCE
    notes
    Uses the committed MIBiG compound inventory, entry version 3, not a new live database refresh. The matching taxon, AP009493.1 locus 1490671..1492896 and 2-methylisoborneol connectivity contextualize the automated enzyme role. The adopted locus remains CLUSTER_UNSTATED.
SGR_1269 (2-methylisoborneol synthase; proposed) is_database_proposed_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) SGR_1269/BAG18098.1, 437 aa. Native-reference assignment in Streptomyces griseus subsp. griseus NBRC 13350 (NCBITaxon:455632), not an identified assayed construct. UniProt automated function and the adopted MIBiG BGC0000658 v3 locus support a proposed biosynthetic role. No new primary experiment, exact reaction, stereochemical assignment, pathway order, shell-cargo relationship or producer/locus evidence upgrade is asserted.
https://www.uniprot.org/uniprotkb/B1VVB4/entry
https://www.ncbi.nlm.nih.gov/protein/BAG18098.1
https://mibig.secondarymetabolites.org/repository/BGC0000658/
  • reference
    https://www.uniprot.org/uniprotkb/B1VVB4/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    Belongs to the terpene synthase family. 2-methylisoborneol synthase subfamily
    retrieved on
    2026-10-08
    notes
    UniProtKB release 2026_03, unreviewed entry 79, sequence 1. Automated ECO:0000256 annotation, not direct experimental evidence for this sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/BAG18098.1
    evidence type
    DATABASE_ASSERTION
    snippet
    SGR_1269
    retrieved on
    2026-10-09
    notes
    Native NBRC 13350 reference, 437 aa, reverse-strand AP009493.1:1491583..1492896. GenPept, UniProt, annotated CDS and table-11 CDS translation agree exactly. Identity evidence only.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000658/
    evidence type
    CURATOR_INFERENCE
    notes
    Uses the committed MIBiG compound inventory, entry version 3, not a new live database refresh. The matching taxon, AP009493.1 locus 1490671..1492896 and 2-methylisoborneol connectivity contextualize the automated enzyme role. The adopted locus remains CLUSTER_UNSTATED.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 3 nodes, 2 edges

Two proposed enzyme roles from the adopted NRRL 2338 locus, distinct from the NBRC 13350 graph. Exact native-reference identities are grounded; function remains database-inferred. No native-strain assay, exact reaction, shell-cargo mapping or stereochemical resolution is added.

SubjectPredicateObjectNotesEvidence
SACE_3721 (geranyl diphosphate 2-C-methyltransferase; proposed) is_database_proposed_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) SACE_3721/CAM02993.1, 285 aa. Native-reference assignment in Saccharopolyspora erythraea NRRL 2338 (NCBITaxon:405948). The UniProt functional annotation is curator inference (ECO:0000305), not an isolated-enzyme assay for this sequence. The adopted MIBiG BGC0000659 v3 locus supports the proposed compound association. No exact assayed construct, reaction, pathway order, stereochemical assignment, shell-cargo relationship or producer/locus evidence upgrade is asserted.
https://www.uniprot.org/uniprotkb/A4FG18/entry
https://www.ncbi.nlm.nih.gov/protein/CAM02993.1
https://mibig.secondarymetabolites.org/repository/BGC0000659/
  • reference
    https://www.uniprot.org/uniprotkb/A4FG18/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    Catalyzes the SAM-dependent methylation of geranyl diphosphate (GPP) to yield (E)-2-methylgeranyl diphosphate (2-MeGPP)
    retrieved on
    2026-10-09
    notes
    UniProtKB release 2026_03, reviewed entry 79, sequence 1. Function uses ECO:0000305 citing PMID:18492804; the database assertion is not newly inspected primary experimental evidence. The IDA process annotation does not establish this exact catalytic reaction.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/CAM02993.1
    evidence type
    DATABASE_ASSERTION
    snippet
    SACE_3721
    retrieved on
    2026-10-09
    notes
    Native NRRL 2338 reference, reverse-strand AM420293.1:4075163..4076020. All 285 residues agree across UniProt, GenPept, the annotated CDS and independent table-11 translation. Identity evidence only.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000659/
    evidence type
    CURATOR_INFERENCE
    notes
    Uses the committed MIBiG compound inventory, entry version 3, not a live refresh. The matching taxon, AM420293.1 locus 4075163..4077099 and 2-methylisoborneol connectivity contextualize the proposed role. SOURCE_ASSERTION and CLUSTER_UNSTATED grades are retained.
SACE_3722 (2-methylisoborneol synthase; proposed) is_database_proposed_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) SACE_3722/CAM02994.1, 354 aa. Native-reference assignment in Saccharopolyspora erythraea NRRL 2338 (NCBITaxon:405948). The UniProt functional annotation is curator inference (ECO:0000305), not an isolated-enzyme assay for this sequence. The adopted MIBiG BGC0000659 v3 locus supports the proposed compound association. No exact assayed construct, reaction, pathway order, stereochemical assignment, shell-cargo relationship or producer/locus evidence upgrade is asserted.
https://www.uniprot.org/uniprotkb/A4FG19/entry
https://www.ncbi.nlm.nih.gov/protein/CAM02994.1
https://mibig.secondarymetabolites.org/repository/BGC0000659/
  • reference
    https://www.uniprot.org/uniprotkb/A4FG19/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB)
    retrieved on
    2026-10-08
    notes
    UniProtKB release 2026_03, reviewed entry 85, sequence 1. Function uses ECO:0000305 citing PMID:18492804; the database assertion is not newly inspected primary experimental evidence. The IDA process annotation does not establish this exact catalytic reaction.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/CAM02994.1
    evidence type
    DATABASE_ASSERTION
    snippet
    SACE_3722
    retrieved on
    2026-10-09
    notes
    Native NRRL 2338 reference, reverse-strand AM420293.1:4076035..4077099. All 354 residues agree across UniProt, GenPept, the annotated CDS and independent table-11 translation. Identity evidence only.
  • reference
    https://mibig.secondarymetabolites.org/repository/BGC0000659/
    evidence type
    CURATOR_INFERENCE
    notes
    Uses the committed MIBiG compound inventory, entry version 3, not a live refresh. The matching taxon, AM420293.1 locus 4075163..4077099 and 2-methylisoborneol connectivity contextualize the proposed role. SOURCE_ASSERTION and CLUSTER_UNSTATED grades are retained.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 3 nodes, 2 edges

Two database-proposed ATCC 23877 enzyme roles, distinct from the existing NBRC 13350 and NRRL 2338 graphs. Two native genomic deposits ground each reference sequence; function remains inferred. No MIBiG locus or producer assertion is added, and no direct assay, reaction order, shell-cargo mapping or stereochemical resolution is claimed.

SubjectPredicateObjectNotesEvidence
SAML0357 (2-methylisoborneol synthase; proposed) is_database_proposed_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) SAML0357/SAM23877_0409, 440 aa. Native-reference protein in Streptomyces ambofaciens ATCC 23877 (NCBITaxon:278992), not an identified assayed construct. UniProt assigns function by curator inference (ECO:0000305) and explicitly links this entry to 2-methylisoborneol biosynthesis. This proposed role does not assert an exact reaction, pathway order, stereochemistry, shell-cargo relationship or new producer/BGC evidence grade.
https://www.uniprot.org/uniprotkb/A3KI17/entry
https://www.ncbi.nlm.nih.gov/protein/CAJ89344.1
https://www.ncbi.nlm.nih.gov/protein/AKZ53458.1
  • reference
    https://www.uniprot.org/uniprotkb/A3KI17/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB)
    retrieved on
    2026-10-08
    notes
    UniProtKB release 2026_03, reviewed entry 57, sequence 1. Function uses ECO:0000305 citing PMID:18492804; its reference annotation explicitly names 2-methylisoborneol biosynthesis. This is an inspected database assertion, not newly inspected primary assay evidence. The IDA process annotation does not establish the exact catalytic reaction.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/CAJ89344.1
    evidence type
    DATABASE_ASSERTION
    snippet
    SAML0357
    retrieved on
    2026-10-09
    notes
    Native ATCC 23877 reference, forward-strand AM238663.1:360632..361954. All 440 residues match UniProt, this GenPept sequence, the annotated CDS and independent table-11 translation. Both genomic mappings agree. Identity evidence, not an enzymatic assay.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AKZ53458.1
    evidence type
    DATABASE_ASSERTION
    snippet
    SAM23877_0409
    retrieved on
    2026-10-09
    notes
    Native ATCC 23877 reference, forward-strand CP012382.1:360601..361923. All 440 residues match UniProt, this GenPept sequence, the annotated CDS and independent table-11 translation. Both genomic mappings agree. Identity evidence, not an enzymatic assay.
SAML0358 (geranyl diphosphate 2-C-methyltransferase; proposed) is_database_proposed_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) SAML0358/SAM23877_0410, 291 aa. Native-reference protein in Streptomyces ambofaciens ATCC 23877 (NCBITaxon:278992), not an identified assayed construct. UniProt assigns function by curator inference (ECO:0000305) and explicitly links this entry to 2-methylisoborneol biosynthesis. This proposed role does not assert an exact reaction, pathway order, stereochemistry, shell-cargo relationship or new producer/BGC evidence grade.
https://www.uniprot.org/uniprotkb/A3KI18/entry
https://www.ncbi.nlm.nih.gov/protein/CAJ89345.1
https://www.ncbi.nlm.nih.gov/protein/AKZ53459.1
  • reference
    https://www.uniprot.org/uniprotkb/A3KI18/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    Catalyzes the SAM-dependent methylation of geranyl diphosphate (GPP) to yield (E)-2-methylgeranyl diphosphate (2-MeGPP)
    retrieved on
    2026-10-09
    notes
    UniProtKB release 2026_03, reviewed entry 53, sequence 1. Function uses ECO:0000305 citing PMID:18492804; its reference annotation explicitly names 2-methylisoborneol biosynthesis. This is an inspected database assertion, not newly inspected primary assay evidence. The IDA process annotation does not establish the exact catalytic reaction.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/CAJ89345.1
    evidence type
    DATABASE_ASSERTION
    snippet
    SAML0358
    retrieved on
    2026-10-09
    notes
    Native ATCC 23877 reference, forward-strand AM238663.1:361970..362845. All 291 residues match UniProt, this GenPept sequence, the annotated CDS and independent table-11 translation. Both genomic mappings agree. Identity evidence, not an enzymatic assay.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AKZ53459.1
    evidence type
    DATABASE_ASSERTION
    snippet
    SAM23877_0410
    retrieved on
    2026-10-09
    notes
    Native ATCC 23877 reference, forward-strand CP012382.1:361939..362814. All 291 residues match UniProt, this GenPept sequence, the annotated CDS and independent table-11 translation. Both genomic mappings agree. Identity evidence, not an enzymatic assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 4 nodes, 3 edges

Three independent recombinant assays, not one native three-enzyme pathway. Canonical references ground the reported proteins; exact inserts, tags, linkers and variants remain outside this claim. The owner retains unspecified stereochemistry and existing source grades.

SubjectPredicateObjectNotesEvidence
ScMIBS (2-methylisoborneol synthase; canonical reference) catalyzes_formation_in_coupled_in_vitro_assay_of 2-methylisoborneol (stereochemistry unspecified) ScMIBS is grounded to the exact 440-aa SCO7700 reference, not a truncated domain or variant. Canonical-reference mapping, not exact cloned-insert/tag/linker reconstruction. This is a separate coupled in-vitro assay, not a shared native pathway. No reaction order, native producer/BGC grade, kinetic value or stereochemical upgrade is asserted.
DOI:10.1002/chem.202300775
https://www.uniprot.org/uniprotkb/Q9F1Y6/entry
https://www.ncbi.nlm.nih.gov/protein/CAD55534.1
https://www.ncbi.nlm.nih.gov/protein/WP_011031839.1
  • reference
    DOI:10.1002/chem.202300775
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    function efficiently as MIBSs
    retrieved on
    2026-10-09
    notes
    ScMIBS/WP_011031839, Streptomyces coelicolor A3(2) DSM 40783; recombinant pYE-Express preparation. Coupled FPPS assay yielded 2-MIB by GC/MS. Main text only; supplement inaccessible.
  • reference
    https://www.uniprot.org/uniprotkb/Q9F1Y6/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    SCO7700
    retrieved on
    2026-10-08
    notes
    Reviewed UniProt entry 113, sequence 1, 440 aa, taxon 100226. Canonical reference, not a reconstruction of the experimental insert or a claim that the assay stock was M145.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/CAD55534.1
    evidence type
    CURATOR_INFERENCE
    snippet
    SCO7700
    retrieved on
    2026-10-09
    notes
    A3(2) native AL939132.1:171530..172852, forward. UniProt, GenPept, the named RefSeq reference, annotated CDS and independent table-11 translation agree exactly. The separate 1147 deposit agrees but does not identify the assay stock.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/WP_011031839.1
    evidence type
    DATABASE_ASSERTION
    snippet
    WP_011031839.1
    retrieved on
    2026-10-09
    notes
    Versioned reference corresponding to the paper's unversioned accession. Sequence identity was independently verified; RefSeq species/multispecies assignment is not an experimental strain record. No shell-cargo claim follows from its automated name.
SeMIBS (2-methylisoborneol synthase; canonical reference) catalyzes_formation_in_coupled_in_vitro_assay_of 2-methylisoborneol (stereochemistry unspecified) SeMIBS is grounded to the exact 362-aa BN6_59350 reference; unreviewed status does not replace the independently cited experiment. Canonical-reference mapping, not exact cloned-insert/tag/linker reconstruction. This is a separate coupled in-vitro assay, not a shared native pathway. No reaction order, native producer/BGC grade, kinetic value or stereochemical upgrade is asserted.
DOI:10.1002/chem.202300775
https://www.uniprot.org/uniprotkb/K0K6P8/entry
https://www.ncbi.nlm.nih.gov/protein/CCH33192.1
https://www.ncbi.nlm.nih.gov/protein/WP_015103303.1
  • reference
    DOI:10.1002/chem.202300775
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    function efficiently as MIBSs
    retrieved on
    2026-10-09
    notes
    SeMIBS/WP_015103303, Saccharothrix espanaensis DSM 44229; recombinant pYE-Express preparation. Coupled FPPS assay yielded 2-MIB by GC/MS. Main text only; supplement inaccessible.
  • reference
    https://www.uniprot.org/uniprotkb/K0K6P8/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    BN6_59350
    retrieved on
    2026-10-09
    notes
    Unreviewed UniProt entry 55, sequence 1, 362 aa, native DSM 44229/taxon 1179773. Identity source only; automated annotation is not the primary functional evidence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/CCH33192.1
    evidence type
    CURATOR_INFERENCE
    snippet
    BN6_59350
    retrieved on
    2026-10-09
    notes
    Native DSM 44229 HE804045.1:6630735..6631823, forward. UniProt, GenPept, the named RefSeq reference, annotated CDS and independent table-11 translation agree exactly.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/WP_015103303.1
    evidence type
    DATABASE_ASSERTION
    snippet
    WP_015103303.1
    retrieved on
    2026-10-09
    notes
    Versioned reference corresponding to the paper's unversioned accession. Sequence identity was independently verified; RefSeq species/multispecies assignment is not an experimental strain record. No shell-cargo claim follows from its automated name.
SpMIBS (2-methylisoborneol synthase; canonical reference) catalyzes_formation_in_coupled_in_vitro_assay_of 2-methylisoborneol (stereochemistry unspecified) SpMIBS uses the exact 438-aa RefSeq reference. A0A918LM63 differs at positions 107 (T/A) and 116 (G/R), so it is not substituted as an exact UniProt mapping. Canonical-reference mapping, not exact cloned-insert/tag/linker reconstruction. This is a separate coupled in-vitro assay, not a shared native pathway. No reaction order, native producer/BGC grade, kinetic value or stereochemical upgrade is asserted.
DOI:10.1002/chem.202300775
https://www.ncbi.nlm.nih.gov/nuccore/NZ_KB913030.1
https://www.ncbi.nlm.nih.gov/protein/WP_019890277.1
https://rest.uniprot.org/uniparc/UPI00037B9776.json
  • reference
    DOI:10.1002/chem.202300775
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    function efficiently as MIBSs
    retrieved on
    2026-10-09
    notes
    SpMIBS/WP_019890277, Streptomyces purpureus DSM 43360; recombinant pYE-Express preparation. Coupled FPPS assay yielded 2-MIB by GC/MS. Main text only; supplement inaccessible.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/NZ_KB913030.1
    evidence type
    CURATOR_INFERENCE
    snippet
    STRPU_RS0127440
    retrieved on
    2026-10-09
    notes
    The 438-aa RefSeq reference equals the annotated and independently table-11-translated CDS at 5956391..5957707, forward, native KA281/ATCC 21405/taxon 1054860. This mapping is not proof of exact assay-stock equivalence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/WP_019890277.1
    evidence type
    DATABASE_ASSERTION
    snippet
    WP_019890277.1
    retrieved on
    2026-10-09
    notes
    Versioned reference corresponding to the paper's unversioned accession. Sequence identity was independently verified; RefSeq species/multispecies assignment is not an experimental strain record. No shell-cargo claim follows from its automated name.
  • reference
    https://rest.uniprot.org/uniparc/UPI00037B9776.json
    evidence type
    DATABASE_ASSERTION
    notes
    UniParc UPI00037B9776 (438 aa) links active RefSeq WP_019890277.1 (Streptomyces purpureus, taxon 1925) and carries zero active or inactive UniProtKB cross-references.
    retrieved on
    2026-10-30

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 2 nodes, 1 edge

An independently database-attributed role, not a shared native pathway with the other reference proteins. The owner retains unspecified stereochemistry and existing source grades.

SubjectPredicateObjectNotesEvidence
SCO7701 (geranyl diphosphate 2-C-methyltransferase; database-supported) is_database_annotated_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) SCO7701/Q9F1Y5, 292 aa, is the native canonical reference. The non-proposed assignment reflects UniProt experimental attribution, not a newly inspected primary assay. The database assigns GPP methylation to 2-MeGPP in methylisoborneol biosynthesis; this edge does not assert that the methyltransferase alone produces 2-MIB. Genomic A3(2), 1147, the UniProt M145 label and the cited NRRL B-16638 stock are not equated. No exact construct, reaction order, kinetics, shell-cargo relationship, native producer/BGC grade or stereochemical upgrade is asserted.
https://www.uniprot.org/uniprotkb/Q9F1Y5/entry
https://www.ncbi.nlm.nih.gov/protein/CAC44557.1
https://www.ncbi.nlm.nih.gov/protein/BAB20434.1
https://www.ncbi.nlm.nih.gov/protein/WP_011031840.1
  • reference
    https://www.uniprot.org/uniprotkb/Q9F1Y5/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    SAM-dependent methylation of geranyl diphosphate (GPP)
    retrieved on
    2026-10-09
    notes
    Reviewed entry 125, sequence 1. Function uses ECO:0000269/PMID:18563898; its publication annotation includes PATHWAY and names methylisoborneol biosynthesis. This is the inspected database assertion, not newly inspected primary evidence. The cited DOI:10.1021/ja803639g and correction DOI:10.1021/ja104306p are access-held; no kinetic values are adopted. The cited assay stock is A3(2)/NRRL B-16638, not established here as identical to the genomic stocks.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/CAC44557.1
    evidence type
    CURATOR_INFERENCE
    snippet
    SCO7701
    retrieved on
    2026-10-09
    notes
    Native A3(2), taxon 100226, forward AL939132.1:172868..173746. All 292 residues match UniProt, GenPept, the annotated CDS and independent table-11 translation. This is canonical-reference identity, not an experimental construct.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/BAB20434.1
    evidence type
    CURATOR_INFERENCE
    snippet
    orf4
    retrieved on
    2026-10-09
    notes
    Native strain 1147, taxon 1902, forward AB035202.1:5872..6750. The CDS and 292-aa translation exactly match the A3(2) reference. Sequence equality does not equate strains or identify the cited assay stock.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/WP_011031840.1
    evidence type
    DATABASE_ASSERTION
    snippet
    WP_011031840.1
    retrieved on
    2026-10-09
    notes
    Exact 292-aa reference; this RefSeq is multispecies (taxon 1883), not a strain assignment.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 2 nodes, 1 edge

An independently database-attributed role, not a shared native pathway with the other reference proteins. The owner retains unspecified stereochemistry and existing source grades.

SubjectPredicateObjectNotesEvidence
Strain-13189 2-methylisoborneol synthase (similarity-inferred) is_database_proposed_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) Q9F1V8/BAB20507.1, 437 aa, is a native strain-13189 reference with a similarity-inferred MIB synthase role, not an assayed protein. Do not identify strain 13189 with NBRC 13350 or borrow BGC0000658. The adjacent putative methyltransferase BAB20508.1 is not assigned a specific MIB function from neighborhood alone. No exact construct, reaction order, kinetics, shell-cargo relationship, native producer/BGC grade or stereochemical upgrade is asserted.
https://www.uniprot.org/uniprotkb/Q9F1V8/entry
https://www.ncbi.nlm.nih.gov/protein/BAB20507.1
https://www.ncbi.nlm.nih.gov/protein/WP_030703894.1
  • reference
    https://www.uniprot.org/uniprotkb/Q9F1V8/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB)
    retrieved on
    2026-10-08
    notes
    Reviewed entry 67, sequence 1, Streptomyces griseus/taxon 1911. Function uses ECO:0000250 (sequence similarity), not experimental evidence for this protein. The sequence-deposit reference names strain 13189 without a collection prefix.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/BAB20507.1
    evidence type
    CURATOR_INFERENCE
    snippet
    13189
    retrieved on
    2026-10-09
    notes
    Native genomic DNA, strain 13189/taxon 1911, forward AB040071.1:5255..6568. All 437 residues match UniProt, GenPept, RefSeq, the annotated CDS and independent table-11 translation. Q9F1V8 differs from NBRC 13350 B1VVB4 at residues 66 (P/S), 67 (T/A) and 77 (W/R); they are not interchangeable references.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/WP_030703894.1
    evidence type
    DATABASE_ASSERTION
    snippet
    WP_030703894.1
    retrieved on
    2026-10-09
    notes
    Exact 437-aa species-level RefSeq, taxon 1911, without a strain qualifier. Its automatic encapsulin-cargo name is not adopted as functional evidence.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 3 nodes, 2 edges

Two database-attributed biosynthetic roles for deposited ATCC 31180 references. This graph is not a newly established native pKSL pathway or a reconstruction of the access-held experiments. Existing chemistry, evidence grades and review status are retained.

SubjectPredicateObjectNotesEvidence
Tpc (2-methylisoborneol synthase; database-supported) is_database_annotated_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) D3KYU2/BAI77523.1/tpc, 481 aa, is a deposited ATCC 31180 reference from Streptomyces lasalocidi (literature name S. lasaliensis), not an established native-locus or exact assay-construct assignment. The non-proposed role reflects UniProt experimental attribution, not a newly inspected primary assay. Use the explicit ATCC 31180 designation; uncertain DSM aliases are not equated. No reaction order, kinetic value, native production, producer/BGC grade, shell-cargo relationship or stereochemical resolution is asserted.
https://www.uniprot.org/uniprotkb/D3KYU2/entry
https://www.ncbi.nlm.nih.gov/protein/BAI77523.1
https://www.ncbi.nlm.nih.gov/nuccore/AB547324.1
  • reference
    https://www.uniprot.org/uniprotkb/D3KYU2/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB)
    notes
    Reviewed entry 40, sequence 1, taxon 324833. FUNCTION uses ECO:0000269/PMID:18492804; its publication annotation includes PATHWAY and ATCC 31180. This is database experimental attribution, not newly inspected primary evidence; the cited DOI:10.1073/pnas.0802312105 remains access-held.
    retrieved on
    2026-10-08
  • reference
    https://www.ncbi.nlm.nih.gov/protein/BAI77523.1
    evidence type
    CURATOR_INFERENCE
    snippet
    tpc
    notes
    Deposited ATCC 31180 reference, 481 aa, forward AB547324.1:1729..3174. UniProt, GenPept, the annotated CDS and independent table-11 translation agree exactly. Sequence identity does not resolve native pKSL versus an experimental clone or reconstruct a cited assay insert.
    retrieved on
    2026-10-09
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AB547324.1
    evidence type
    DATABASE_ASSERTION
    snippet
    ATCC 31180
    notes
    The deposit explicitly names this strain, taxon 324833 and plasmid pKSL, with mol_type genomic DNA. These annotations alone do not resolve the plasmid provenance. No function is inferred for neighboring nbp.
    retrieved on
    2026-10-09
Gdpmt (geranyl diphosphate 2-C-methyltransferase; database-supported) is_database_annotated_biosynthetic_enzyme_for 2-methylisoborneol (stereochemistry unspecified) D3KYU3/BAI77524.1/gdpmt, 300 aa, is a deposited ATCC 31180 reference from Streptomyces lasalocidi (literature name S. lasaliensis), not an established native-locus or exact assay-construct assignment. The non-proposed role reflects UniProt experimental attribution, not a newly inspected primary assay. Use the explicit ATCC 31180 designation; uncertain DSM aliases are not equated. No reaction order, kinetic value, native production, producer/BGC grade, shell-cargo relationship or stereochemical resolution is asserted. The methyltransferase role does not assert that this enzyme alone produces 2-MIB.
https://www.uniprot.org/uniprotkb/D3KYU3/entry
https://www.ncbi.nlm.nih.gov/protein/BAI77524.1
https://www.ncbi.nlm.nih.gov/nuccore/AB547324.1
DOI:10.2210/pdb4F84/pdb
  • reference
    https://www.uniprot.org/uniprotkb/D3KYU3/entry
    evidence type
    DATABASE_ASSERTION
    snippet
    SAM-dependent methylation of geranyl diphosphate (GPP)
    notes
    Reviewed entry 47, sequence 1, taxon 324833. FUNCTION uses ECO:0000269/PMID:18492804; its publication annotation includes PATHWAY and ATCC 31180. This is database experimental attribution, not newly inspected primary evidence; the cited DOI:10.1073/pnas.0802312105 remains access-held.
    retrieved on
    2026-10-09
  • reference
    https://www.ncbi.nlm.nih.gov/protein/BAI77524.1
    evidence type
    CURATOR_INFERENCE
    snippet
    gdpmt
    notes
    Deposited ATCC 31180 reference, 300 aa, forward AB547324.1:3219..4121. UniProt, GenPept, the annotated CDS and independent table-11 translation agree exactly. Sequence identity does not resolve native pKSL versus an experimental clone or reconstruct a cited assay insert.
    retrieved on
    2026-10-09
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/AB547324.1
    evidence type
    DATABASE_ASSERTION
    snippet
    ATCC 31180
    notes
    The deposit explicitly names this strain, taxon 324833 and plasmid pKSL, with mol_type genomic DNA. These annotations alone do not resolve the plasmid provenance. No function is inferred for neighboring nbp.
    retrieved on
    2026-10-09
  • reference
    DOI:10.2210/pdb4F84/pdb
    evidence type
    CURATOR_INFERENCE
    snippet
    gdpmt
    notes
    Independent PDB4F84 entity 1 is exactly a 20-aa MGSSHHHHHHSSGLVPRGSH extension plus all 300 canonical D3KYU3 residues. SIFTS maps entity 21..320 to reference 1..300; mutation count zero. Source taxon 324833 has no strain qualifier; expression is pET26b in E. coli BL21(DE3). Identity evidence only, not product formation, proteolysis or native assembly evidence. The related primary paper remains access-held.
    retrieved on
    2026-10-09

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (2-methylisoborneol) ↗ MediaIngredientMech (naturalproductmech:mibig-0eb8c93c6f) ↗ TaxonMech: Streptomyces griseus subsp. griseus NBRC 13350 (NCBITaxon:455632) ↗ TaxonMech: Saccharopolyspora erythraea NRRL 2338 (NCBITaxon:405948) ↗ PathwayMech (mibig:BGC0000658) ↗ PathwayMech (mibig:BGC0000659) ↗

Open questions 1

shared-structure — This structure is reported by more than one MIBiG entry: BGC0000658, BGC0000659. Confirm they describe the same compound rather than an upstream cross-reference error.

Provenance

Source conceptSourceVersion
BGC0000658MIBIG3
BGC0000659MIBIG3

This page is generated from data/natural_products/terpenoids/2-methylisoborneol.yaml, which is generated from the committed inventories. Neither is edited by hand.