jamaicamide B

naturalproductmech:mibig-e0dfe0eb89 MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	KZVHAGNFWJIOMX-GDKFLGFMSA-N
SMILES	CO\C(CCNC(=O)CC\C=C\C(C)CC\C(CCCC#C)=C\Cl)=C\C(=O)N1C(C)C=CC1=O
Stereochemistry	undefined stereocentres — the InChIKey does not distinguish this compound from its stereoisomers
Cross-references	cyanometdb:CyanoMetDB_1358, pubchem:49787032, npatlas:NPA0374

Filing pathway

Unclassified — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is nrps, pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Lyngbya majuscula NCBITaxon:158786	SOURCE_ASSERTION	mibig:BGC0001001	DOI:10.1016/j.chembiol.2004.03.030

Occurrences 1

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

Taxon	Source	Reference
Lyngbya majuscula NCBITaxon:158786	LOTUS	DOI:10.1016/J.CHEMBIOL.2004.03.030

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0001001	Lyngbya majuscula NCBITaxon:158786	CLUSTER_UNSTATED	genbank:AY522504.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Biosynthesis curated

Written by a curator with claim-level evidence on each step; not derived from a source. This is what the rest of the record exists to support.

#	Enzyme	Reaction	Substrate → product	Evidence
1	JamA starter-acid activating/loading ligase (UniProt annotation) UniProtKB:Q6E7K9		ungrounded → ungrounded	https://www.uniprot.org/uniprotkb/Q6E7K9/entry
2	JamB carrier-bound acetylenase (UniProt annotation) UniProtKB:Q6E7K8		ungrounded → ungrounded	https://www.uniprot.org/uniprotkb/Q6E7K8/entry

Entry 1: Database-described activation of 5-hexenoic acid and loading onto the distinct JamC carrier to form 5-hexenoyl-JamC. JamC is not an enzyme step. This partial starter-unit mechanism is a UniProt database assertion; its contribution to the exact jamaicamide B owner is a curator inference, not an independently inspected compound-specific experiment. The inference combines explicit jamaicamide starter-unit function, exact native cluster sequence identity, the pinned MIBiG association of B with AY522504.1, and the owner's compatible terminal alkyne. Neither gene neighborhood nor fragment compatibility alone proves the assignment. No atom mapping, intact C6 incorporation proof, final-product synthesis assay, native production/knockout result, balanced reaction, assay construct, or complete numbered pathway is asserted. Carrier-bound intermediates remain ungrounded and distinct from free acids and the final owner. The owner's three alkene configurations and two unspecified tetrahedral centers, source taxon, producer/BGC grades and SEEDED status are unchanged.

Entry 2: Database-described 5-hexenoyl-JamC to 5-hexynoyl-JamC acetylenation; neither free acid nor final jamaicamide B is a direct substrate/product of this represented step. This partial starter-unit mechanism is a UniProt database assertion; its contribution to the exact jamaicamide B owner is a curator inference, not an independently inspected compound-specific experiment. The inference combines explicit jamaicamide starter-unit function, exact native cluster sequence identity, the pinned MIBiG association of B with AY522504.1, and the owner's compatible terminal alkyne. Neither gene neighborhood nor fragment compatibility alone proves the assignment. No atom mapping, intact C6 incorporation proof, final-product synthesis assay, native production/knockout result, balanced reaction, assay construct, or complete numbered pathway is asserted. Carrier-bound intermediates remain ungrounded and distinct from free acids and the final owner. The owner's three alkene configurations and two unspecified tetrahedral centers, source taxon, producer/BGC grades and SEEDED status are unchanged.

Mechanism & Biosynthesis Overview

Causal graph — biosynthesis 9 nodes, 8 edges

Partial JHB JamABC starter-unit graph. UniProt describes JamA/JamC loading and JamB acetylenation; the exact jamaicamide B connection is explicitly inferred from the combined source/cluster/chemical context, not a verified complete synthesis. Native protein identities are sequence-checked; primary experiments and constructs are uninspected.

Subject	Predicate	Object	Notes	Evidence
5-Hexenoic acid (free acid; source name)	is_database_asserted_substrate_of	Starter-acid activation and loading onto JamC	Free 5-hexenoic acid enters JamA activation/loading, not the JamB step. The free acid is not equated with a carrier-bound intermediate.	https://www.uniprot.org/uniprotkb/Q6E7K9/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.uniprot.org/uniprotkb/Q6E7K7/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 reference https://www.uniprot.org/uniprotkb/Q6E7K9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet the loading of the activated 5-hexenoic acid onto the acyl carrier protein JamC notes Reviewed UniProtKB Q6E7K9, entry 64/sequence 1, annotation 2026-09-02. UniProt assigns JamA activation of the free 5-hexenoic-acid starter followed by loading onto JamC. This is a ligase assignment, not a claim that JamA alone synthesizes the final natural product. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamA: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 6630..8408 (1-based inclusive), codon_start 1/table 11, translates exactly to the 592-aa GenBank CDS annotation AAS98774.1 and UniProtKB:Q6E7K9. UniProt explicitly cross-references AY522504/AAS98774.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.uniprot.org/uniprotkb/Q6E7K7/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Accepts as substrate the activated form of 5-hexenoic acid from JamA notes Reviewed UniProtKB Q6E7K7, entry 75/sequence 1, annotation 2026-09-02. UniProt assigns JamC acyl-carrier function and acceptance of activated 5-hexenoic acid from JamA. JamC is a carrier protein, not a separate enzyme or an independently inferred catalytic step. The entry's protein-existence field is Evidence at transcript level. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamC: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 9718..10020 (1-based inclusive), codon_start 1/table 11, translates exactly to the 100-aa GenBank CDS annotation AAS98798.1 and UniProtKB:Q6E7K7. UniProt explicitly cross-references AY522504/AAS98798.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Lyngbya majuscula JHB notes NCBI's strain page lists this heterotypic synonym for Moorena producens JHB, NCBITaxon:1454205, the strain identity used by the reviewed JamABC UniProt entries. The older nucleotide source and seeded record use Lyngbya majuscula, NCBITaxon:158786. The strain-specific naming bridge and exact accession/sequence matches do not equate those taxon IDs or replace the owner's source-owned taxonomy assertions.
Moorena producens JHB JamA ligase	is_database_assigned_catalyst_of	Starter-acid activation and loading onto JamC	UniProt's starter-acid activation/loading assignment. This is not final jamaicamide synthesis by JamA or an independently read primary assay.	https://www.uniprot.org/uniprotkb/Q6E7K9/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.uniprot.org/uniprotkb/Q6E7K7/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 reference https://www.uniprot.org/uniprotkb/Q6E7K9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet the loading of the activated 5-hexenoic acid onto the acyl carrier protein JamC notes Reviewed UniProtKB Q6E7K9, entry 64/sequence 1, annotation 2026-09-02. UniProt assigns JamA activation of the free 5-hexenoic-acid starter followed by loading onto JamC. This is a ligase assignment, not a claim that JamA alone synthesizes the final natural product. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamA: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 6630..8408 (1-based inclusive), codon_start 1/table 11, translates exactly to the 592-aa GenBank CDS annotation AAS98774.1 and UniProtKB:Q6E7K9. UniProt explicitly cross-references AY522504/AAS98774.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.uniprot.org/uniprotkb/Q6E7K7/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Accepts as substrate the activated form of 5-hexenoic acid from JamA notes Reviewed UniProtKB Q6E7K7, entry 75/sequence 1, annotation 2026-09-02. UniProt assigns JamC acyl-carrier function and acceptance of activated 5-hexenoic acid from JamA. JamC is a carrier protein, not a separate enzyme or an independently inferred catalytic step. The entry's protein-existence field is Evidence at transcript level. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamC: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 9718..10020 (1-based inclusive), codon_start 1/table 11, translates exactly to the 100-aa GenBank CDS annotation AAS98798.1 and UniProtKB:Q6E7K7. UniProt explicitly cross-references AY522504/AAS98798.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Lyngbya majuscula JHB notes NCBI's strain page lists this heterotypic synonym for Moorena producens JHB, NCBITaxon:1454205, the strain identity used by the reviewed JamABC UniProt entries. The older nucleotide source and seeded record use Lyngbya majuscula, NCBITaxon:158786. The strain-specific naming bridge and exact accession/sequence matches do not equate those taxon IDs or replace the owner's source-owned taxonomy assertions.
Moorena producens JHB JamC acyl carrier	is_database_asserted_acyl_carrier_acceptor_for	Starter-acid activation and loading onto JamC	JamC supplies the carrier, not a separate catalytic reaction. No binding constant, direct protein-interaction assay or uninspected experimental phosphopantetheinylation result is inferred.	https://www.uniprot.org/uniprotkb/Q6E7K9/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.uniprot.org/uniprotkb/Q6E7K7/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 reference https://www.uniprot.org/uniprotkb/Q6E7K9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet the loading of the activated 5-hexenoic acid onto the acyl carrier protein JamC notes Reviewed UniProtKB Q6E7K9, entry 64/sequence 1, annotation 2026-09-02. UniProt assigns JamA activation of the free 5-hexenoic-acid starter followed by loading onto JamC. This is a ligase assignment, not a claim that JamA alone synthesizes the final natural product. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamA: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 6630..8408 (1-based inclusive), codon_start 1/table 11, translates exactly to the 592-aa GenBank CDS annotation AAS98774.1 and UniProtKB:Q6E7K9. UniProt explicitly cross-references AY522504/AAS98774.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.uniprot.org/uniprotkb/Q6E7K7/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Accepts as substrate the activated form of 5-hexenoic acid from JamA notes Reviewed UniProtKB Q6E7K7, entry 75/sequence 1, annotation 2026-09-02. UniProt assigns JamC acyl-carrier function and acceptance of activated 5-hexenoic acid from JamA. JamC is a carrier protein, not a separate enzyme or an independently inferred catalytic step. The entry's protein-existence field is Evidence at transcript level. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamC: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 9718..10020 (1-based inclusive), codon_start 1/table 11, translates exactly to the 100-aa GenBank CDS annotation AAS98798.1 and UniProtKB:Q6E7K7. UniProt explicitly cross-references AY522504/AAS98798.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Lyngbya majuscula JHB notes NCBI's strain page lists this heterotypic synonym for Moorena producens JHB, NCBITaxon:1454205, the strain identity used by the reviewed JamABC UniProt entries. The older nucleotide source and seeded record use Lyngbya majuscula, NCBITaxon:158786. The strain-specific naming bridge and exact accession/sequence matches do not equate those taxon IDs or replace the owner's source-owned taxonomy assertions.
Starter-acid activation and loading onto JamC	has_database_asserted_product	5-Hexenoyl-JamC (covalently carrier-bound)	Loaded 5-hexenoyl-JamC as named by UniProt. No free-acid, generic ACP or final-owner identifier is substituted for this covalently loaded state.	https://www.uniprot.org/uniprotkb/Q6E7K9/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.uniprot.org/uniprotkb/Q6E7K7/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 reference https://www.uniprot.org/uniprotkb/Q6E7K9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet the loading of the activated 5-hexenoic acid onto the acyl carrier protein JamC notes Reviewed UniProtKB Q6E7K9, entry 64/sequence 1, annotation 2026-09-02. UniProt assigns JamA activation of the free 5-hexenoic-acid starter followed by loading onto JamC. This is a ligase assignment, not a claim that JamA alone synthesizes the final natural product. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamA: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 6630..8408 (1-based inclusive), codon_start 1/table 11, translates exactly to the 592-aa GenBank CDS annotation AAS98774.1 and UniProtKB:Q6E7K9. UniProt explicitly cross-references AY522504/AAS98774.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.uniprot.org/uniprotkb/Q6E7K7/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Accepts as substrate the activated form of 5-hexenoic acid from JamA notes Reviewed UniProtKB Q6E7K7, entry 75/sequence 1, annotation 2026-09-02. UniProt assigns JamC acyl-carrier function and acceptance of activated 5-hexenoic acid from JamA. JamC is a carrier protein, not a separate enzyme or an independently inferred catalytic step. The entry's protein-existence field is Evidence at transcript level. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamC: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 9718..10020 (1-based inclusive), codon_start 1/table 11, translates exactly to the 100-aa GenBank CDS annotation AAS98798.1 and UniProtKB:Q6E7K7. UniProt explicitly cross-references AY522504/AAS98798.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Lyngbya majuscula JHB notes NCBI's strain page lists this heterotypic synonym for Moorena producens JHB, NCBITaxon:1454205, the strain identity used by the reviewed JamABC UniProt entries. The older nucleotide source and seeded record use Lyngbya majuscula, NCBITaxon:158786. The strain-specific naming bridge and exact accession/sequence matches do not equate those taxon IDs or replace the owner's source-owned taxonomy assertions.
5-Hexenoyl-JamC (covalently carrier-bound)	is_database_asserted_substrate_of	Carrier-bound terminal acetylenation	The JamB substrate is carrier-bound. UniProt explicitly says JamB cannot use free 5-hexenoic acid or 5-hexenoyl-CoA.	https://www.uniprot.org/uniprotkb/Q6E7K8/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 reference https://www.uniprot.org/uniprotkb/Q6E7K8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Catalyzes the conversion of 5-hexenoyl loaded onto the acyl carrier protein JamC (5-hexenoyl-JamC) to 5-hexynoyl-JamC notes Reviewed UniProtKB Q6E7K8, entry 63/sequence 1, annotation 2026-09-02. UniProt assigns JamB acetylenation of carrier-bound 5-hexenoyl-JamC to 5-hexynoyl-JamC. It explicitly excludes free 5-hexenoic acid and 5-hexenoyl-CoA as substrates. Its additional hexanoyl-JamC desaturation annotation is not depicted in this partial branch. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamB: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 8591..9556 (1-based inclusive), codon_start 1/table 11, translates exactly to the 321-aa GenBank CDS annotation AAS98775.1 and UniProtKB:Q6E7K8. UniProt explicitly cross-references AY522504/AAS98775.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Lyngbya majuscula JHB notes NCBI's strain page lists this heterotypic synonym for Moorena producens JHB, NCBITaxon:1454205, the strain identity used by the reviewed JamABC UniProt entries. The older nucleotide source and seeded record use Lyngbya majuscula, NCBITaxon:158786. The strain-specific naming bridge and exact accession/sequence matches do not equate those taxon IDs or replace the owner's source-owned taxonomy assertions.
Moorena producens JHB JamB acetylenase	is_database_assigned_catalyst_of	Carrier-bound terminal acetylenation	UniProt's acetylenation assignment for native JamB. Its separately annotated hexanoyl-JamC desaturation branch is not represented here; no cofactor stoichiometry or native assay construct is asserted.	https://www.uniprot.org/uniprotkb/Q6E7K8/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 reference https://www.uniprot.org/uniprotkb/Q6E7K8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Catalyzes the conversion of 5-hexenoyl loaded onto the acyl carrier protein JamC (5-hexenoyl-JamC) to 5-hexynoyl-JamC notes Reviewed UniProtKB Q6E7K8, entry 63/sequence 1, annotation 2026-09-02. UniProt assigns JamB acetylenation of carrier-bound 5-hexenoyl-JamC to 5-hexynoyl-JamC. It explicitly excludes free 5-hexenoic acid and 5-hexenoyl-CoA as substrates. Its additional hexanoyl-JamC desaturation annotation is not depicted in this partial branch. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamB: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 8591..9556 (1-based inclusive), codon_start 1/table 11, translates exactly to the 321-aa GenBank CDS annotation AAS98775.1 and UniProtKB:Q6E7K8. UniProt explicitly cross-references AY522504/AAS98775.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Lyngbya majuscula JHB notes NCBI's strain page lists this heterotypic synonym for Moorena producens JHB, NCBITaxon:1454205, the strain identity used by the reviewed JamABC UniProt entries. The older nucleotide source and seeded record use Lyngbya majuscula, NCBITaxon:158786. The strain-specific naming bridge and exact accession/sequence matches do not equate those taxon IDs or replace the owner's source-owned taxonomy assertions.
Carrier-bound terminal acetylenation	has_database_asserted_product	5-Hexynoyl-JamC (covalently carrier-bound)	The database-named product is 5-hexynoyl-JamC, not free 5-hexynoic acid and not jamaicamide B. Loaded-intermediate chemistry is ungrounded.	https://www.uniprot.org/uniprotkb/Q6E7K8/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 reference https://www.uniprot.org/uniprotkb/Q6E7K8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Catalyzes the conversion of 5-hexenoyl loaded onto the acyl carrier protein JamC (5-hexenoyl-JamC) to 5-hexynoyl-JamC notes Reviewed UniProtKB Q6E7K8, entry 63/sequence 1, annotation 2026-09-02. UniProt assigns JamB acetylenation of carrier-bound 5-hexenoyl-JamC to 5-hexynoyl-JamC. It explicitly excludes free 5-hexenoic acid and 5-hexenoyl-CoA as substrates. Its additional hexanoyl-JamC desaturation annotation is not depicted in this partial branch. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamB: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 8591..9556 (1-based inclusive), codon_start 1/table 11, translates exactly to the 321-aa GenBank CDS annotation AAS98775.1 and UniProtKB:Q6E7K8. UniProt explicitly cross-references AY522504/AAS98775.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Lyngbya majuscula JHB notes NCBI's strain page lists this heterotypic synonym for Moorena producens JHB, NCBITaxon:1454205, the strain identity used by the reviewed JamABC UniProt entries. The older nucleotide source and seeded record use Lyngbya majuscula, NCBITaxon:158786. The strain-specific naming bridge and exact accession/sequence matches do not equate those taxon IDs or replace the owner's source-owned taxonomy assertions.
5-Hexynoyl-JamC (covalently carrier-bound)	is_inferred_upstream_precursor_for	Jamaicamide B (exact owner; incomplete stereochemistry)	This partial starter-unit mechanism is a UniProt database assertion; its contribution to the exact jamaicamide B owner is a curator inference, not an independently inspected compound-specific experiment. The inference combines explicit jamaicamide starter-unit function, exact native cluster sequence identity, the pinned MIBiG association of B with AY522504.1, and the owner's compatible terminal alkyne. Neither gene neighborhood nor fragment compatibility alone proves the assignment. No atom mapping, intact C6 incorporation proof, final-product synthesis assay, native production/knockout result, balanced reaction, assay construct, or complete numbered pathway is asserted. Carrier-bound intermediates remain ungrounded and distinct from free acids and the final owner. The owner's three alkene configurations and two unspecified tetrahedral centers, source taxon, producer/BGC grades and SEEDED status are unchanged.	https://www.uniprot.org/uniprotkb/Q6E7K8/entry https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 https://github.com/CultureBotAI/NaturalProductMech/blob/ec47f1839841f4be14e4d798510bfc30eb2906fe/data/raw/mibig_compounds.tsv https://github.com/CultureBotAI/NaturalProductMech/blob/ec47f1839841f4be14e4d798510bfc30eb2906fe/data/raw/mibig_compounds.tsv reference https://www.uniprot.org/uniprotkb/Q6E7K8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Catalyzes the conversion of 5-hexenoyl loaded onto the acyl carrier protein JamC (5-hexenoyl-JamC) to 5-hexynoyl-JamC notes Reviewed UniProtKB Q6E7K8, entry 63/sequence 1, annotation 2026-09-02. UniProt assigns JamB acetylenation of carrier-bound 5-hexenoyl-JamC to 5-hexynoyl-JamC. It explicitly excludes free 5-hexenoic acid and 5-hexenoyl-CoA as substrates. Its additional hexanoyl-JamC desaturation annotation is not depicted in this partial branch. The cited primary papers DOI:10.1016/j.chembiol.2004.03.030 and DOI:10.1038/nchembio.1718 were not inspected; database experimental evidence codes are not promoted to newly verified primary experiments. reference https://www.ncbi.nlm.nih.gov/nuccore/AY522504.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes Native-reference audit for jamB: AY522504.1 is the 69,900-nt JHB cluster reference named by BGC0001001. Complete plus-strand CDS 8591..9556 (1-based inclusive), codon_start 1/table 11, translates exactly to the 321-aa GenBank CDS annotation AAS98775.1 and UniProtKB:Q6E7K8. UniProt explicitly cross-references AY522504/AAS98775.1 and the JHB nucleotide report. This is not an independently downloaded GenPept comparison or proof of historical assay-stock/construct identity, function, chemical incorporation or native production. reference https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1454205 evidence type DATABASE_ASSERTION retrieved on 2026-10-08 snippet Lyngbya majuscula JHB notes NCBI's strain page lists this heterotypic synonym for Moorena producens JHB, NCBITaxon:1454205, the strain identity used by the reviewed JamABC UniProt entries. The older nucleotide source and seeded record use Lyngbya majuscula, NCBITaxon:158786. The strain-specific naming bridge and exact accession/sequence matches do not equate those taxon IDs or replace the owner's source-owned taxonomy assertions. reference https://github.com/CultureBotAI/NaturalProductMech/blob/ec47f1839841f4be14e4d798510bfc30eb2906fe/data/raw/mibig_compounds.tsv evidence type DATABASE_ASSERTION retrieved on 2026-10-08 notes Already-held pinned MIBiG inventory, BGC0001001 entry version 5, compound index 2: jamaicamide B, KZVHAGNFWJIOMX-GDKFLGFMSA-N, native reference AY522504.1, active/questionable/complete. Its source citation is DOI:10.1016/j.chembiol.2004.03.030; no locus experiment is stated, so CLUSTER_UNSTATED and SOURCE_ASSERTION grades remain. The live MIBiG page was inaccessible; no current-live-entry or newly verified primary-paper claim is made. reference https://github.com/CultureBotAI/NaturalProductMech/blob/ec47f1839841f4be14e4d798510bfc30eb2906fe/data/raw/mibig_compounds.tsv evidence type CURATOR_INFERENCE retrieved on 2026-10-08 notes This partial starter-unit mechanism is a UniProt database assertion; its contribution to the exact jamaicamide B owner is a curator inference, not an independently inspected compound-specific experiment. The inference combines explicit jamaicamide starter-unit function, exact native cluster sequence identity, the pinned MIBiG association of B with AY522504.1, and the owner's compatible terminal alkyne. Neither gene neighborhood nor fragment compatibility alone proves the assignment. No atom mapping, intact C6 incorporation proof, final-product synthesis assay, native production/knockout result, balanced reaction, assay construct, or complete numbered pathway is asserted. Carrier-bound intermediates remain ungrounded and distinct from free acids and the final owner. The owner's three alkene configurations and two unspecified tetrahedral centers, source taxon, producer/BGC grades and SEEDED status are unchanged. RDKit 2026.03.5 confirms one terminal alkyne in the exact B owner, while A has a brominated alkyne and C an alkene. This is compatibility, not atom-mapped incorporation or full-structure equivalence to 5-hexynoyl-JamC; B is not a direct JamB reaction product.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (jamaicamide B) ↗ MediaIngredientMech (naturalproductmech:mibig-e0dfe0eb89) ↗ TaxonMech: Lyngbya majuscula (NCBITaxon:158786) ↗ PathwayMech (mibig:BGC0001001) ↗

Provenance

Source concept	Source	Version
BGC0001001	MIBIG	5

This page is generated from data/natural_products/unclassified/jamaicamide-b.yaml, which is generated from the committed inventories. Neither is edited by hand.