pyochelin biosynthetic process

GO:0042864

The complete eleven-object MetaCyc PWY-6408 thiotemplate pathway is represented from supplied salicylate and two L-cysteines through pyochelin release. The PAO1 implementation uses PchD, PchE, PchF and PchG; it does not include upstream PchA/PchB salicylate synthesis. The shared cysteine-adenylation reaction turns twice per formal product. Carrier-class coefficients count reactive sites, not whole polypeptides: PchE has distinct aryl and peptidyl carrier domains on one chain. The three-ATP total is a stoichiometric derivation, not a measured yield. Native CPD-9992 specifies the pyochelin I monoanion, one member of the experimentally observed interconverting I/II products. Its use preserves a source-defined I projection; it does not assert exclusive I production, identity with the neutral I/II class, or a separate experimentally resolved release stereoselectivity. Native carrier names do not specify every stereocenter. PchE epimerization occurs after amide formation; its coupled cyclization step retains that scope without adding an unsupported separate reaction. Direct 2022 PAO1 structural work observes a PchE homodimer and bound magnesium; functional enables edges do not assert monomer sufficiency or a stable PchDEFG heterotetramer. The 1999 deposited PchD/E/F sequences are from PA14 and differ from current PAO1; the older PAO1 PchF submission also differs at six residues. Later PAO1 source and construct evidence is distinguished from exact engineered-sequence identity. Current PchD and some PchE/F mechanistic annotations retain similarity or curator inference. PchG is an unreviewed current entry, supported separately by PAO1 genetics and biochemical work; free-analogue measurements are not full carrier-substrate kinetics. PchC editing, transport, iron loading and regulation are outside this native assembly boundary.

Pathway type

pyochelin biosynthesis

Organism scope

Pseudomonas aeruginosa PAO1 NCBITaxon:208964

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Pathway network

42 nodes · 91 asserted directed edges

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Pseudomonas aeruginosa PAO1 `NCBITaxon:208964`	taxon
S-adenosyl-L-homocysteine zwitterion `CHEBI:57856`	small molecule
adenosine 5'-monophosphate(2-) `CHEBI:456215`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
salicylate `CHEBI:30762`	small molecule
2-hydroxybenzoyl-AMP(1-) `CHEBI:86458`	small molecule
L-cysteinyl-AMP zwitterion `CHEBI:144924`	small molecule
pyochelin `MetaCyc:CPD-9992`	small molecule
L-cysteine zwitterion `CHEBI:35235`	small molecule
hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] `MetaCyc:Hydroxyphenyl-bis-thiazolinyl-PchF`	chemical entity
an L-cysteinyl-[PchE non-ribosomal peptide synthase] `MetaCyc:L-cysteinyl-PchE`	chemical entity
an L-cysteinyl-[PchF non-ribosomal peptide synthase] `MetaCyc:L-cysteinyl-PchF`	chemical entity
N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] `MetaCyc:N-desmethyl-pyochelin-PchF`	chemical entity
NADP(3-) `CHEBI:58349`	small molecule
NADPH(4-) `CHEBI:57783`	small molecule
diphosphate(3-) `CHEBI:33019`	small molecule
hydron `CHEBI:15378`	small molecule
a [PchE non-ribosomal peptide synthase] `MetaCyc:PchE`	chemical entity
a [PchF non-ribosomal peptide synthase protein] `MetaCyc:PchF`	chemical entity
pyochelinyl-[PchF non-ribosomal peptide synthase] `MetaCyc:Pyochelinyl-PchF`	chemical entity
S-adenosyl-L-methionine zwitterion `CHEBI:59789`	small molecule
salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] `MetaCyc:Salicyl-L-cysteinyl-PchE`	chemical entity
a salicyl-[PchE non-ribosomal peptide synthase] `MetaCyc:Salicyl-PchE`	chemical entity
water `CHEBI:15377`	small molecule
hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] `MetaCyc:hydroxyphenyl-thiazolinyl-PchE`	chemical entity
Pyochelin synthase PchD `UniProtKB:Q9HWG3`	protein
Pyochelin synthetase PchE `UniProtKB:G3XCV2`	protein
Pyochelin synthetase PchF `UniProtKB:Q9HWG4`	protein
Pyochelin biosynthetic protein PchG `UniProtKB:Q9HWG5`	protein
magnesium(2+) `CHEBI:18420`	cofactor
pantetheine 4'-phosphate(2-) `CHEBI:47942`	cofactor
salicylate + ATP + H+ -> (salicyl)adenylate + diphosphate `MetaCyc:RXN-11113`	molecular activity	left to right
L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	molecular activity	left to right
(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	molecular activity	left to right
salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] -> hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] + H2O `MetaCyc:RXN-11118`	molecular activity	left to right
an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	molecular activity	left to right
hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	molecular activity	left to right
S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	molecular activity	left to right
pyochelinyl-[PchF non-ribosomal peptide synthase] + H2O -> pyochelin + a [PchF non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-11123`	molecular activity	left to right
(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	molecular activity	left to right
(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	molecular activity	left to right
a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
hydron `CHEBI:15378`	consumes	salicylate + ATP + H+ -> (salicyl)adenylate + diphosphate `MetaCyc:RXN-11113`	MetaCyc:RXN-11113 Source assertion: The native left side explicitly contains PROTON with coefficient 1; direction is LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11113"]/left[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
salicylate `CHEBI:30762`	consumes	salicylate + ATP + H+ -> (salicyl)adenylate + diphosphate `MetaCyc:RXN-11113`	MetaCyc:RXN-11113 Source assertion: The native left side explicitly contains CPD-110 with coefficient 1; direction is LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11113"]/left[/@frameid="CPD-110"]; reaction-direction` MetaCyc:CPD-110 Source assertion:* The original CPD-110 structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:30762. Source location `ptools-xml/Compound[@frameid="CPD-110"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:30762 Source assertion: CHEBI:30762 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:30762; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
ATP(4-) `CHEBI:30616`	consumes	salicylate + ATP + H+ -> (salicyl)adenylate + diphosphate `MetaCyc:RXN-11113`	MetaCyc:RXN-11113 Source assertion: The native left side explicitly contains ATP with coefficient 1; direction is LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11113"]/left[/@frameid="ATP"]; reaction-direction` MetaCyc:ATP Source assertion:* The original ATP structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:30616. Source location `ptools-xml/Compound[@frameid="ATP"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:30616 Source assertion: CHEBI:30616 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:30616; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
salicylate + ATP + H+ -> (salicyl)adenylate + diphosphate `MetaCyc:RXN-11113`	produces	2-hydroxybenzoyl-AMP(1-) `CHEBI:86458`	MetaCyc:RXN-11113 Source assertion: The native right side explicitly contains CPD-12084 with coefficient 1; direction is LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11113"]/right[/@frameid="CPD-12084"]; reaction-direction` MetaCyc:CPD-12084 Source assertion:* The original CPD-12084 structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:86458. Source location `ptools-xml/Compound[@frameid="CPD-12084"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:86458 Source assertion: CHEBI:86458 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:86458; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
salicylate + ATP + H+ -> (salicyl)adenylate + diphosphate `MetaCyc:RXN-11113`	produces	diphosphate(3-) `CHEBI:33019`	MetaCyc:RXN-11113 Source assertion: The native right side explicitly contains PPI with coefficient 1; direction is LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11113"]/right[/@frameid="PPI"]; reaction-direction` MetaCyc:PPI Source assertion:* The original PPI structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:33019. Source location `ptools-xml/Compound[@frameid="PPI"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:33019 Source assertion: CHEBI:33019 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:33019; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
L-cysteine zwitterion `CHEBI:35235`	consumes	L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	MetaCyc:RXN-11115 Source assertion: The native left side explicitly contains CYS with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11115"]/left[/@frameid="CYS"]; reaction-direction` MetaCyc:CYS Source assertion:* The original CYS structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:35235. Source location `ptools-xml/Compound[@frameid="CYS"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:35235 Source assertion: CHEBI:35235 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:35235; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
ATP(4-) `CHEBI:30616`	consumes	L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	MetaCyc:RXN-11115 Source assertion: The native left side explicitly contains ATP with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11115"]/left[/@frameid="ATP"]; reaction-direction` MetaCyc:ATP Source assertion:* The original ATP structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:30616. Source location `ptools-xml/Compound[@frameid="ATP"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:30616 Source assertion: CHEBI:30616 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:30616; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
hydron `CHEBI:15378`	consumes	L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	MetaCyc:RXN-11115 Source assertion: The native left side explicitly contains PROTON with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11115"]/left[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	produces	L-cysteinyl-AMP zwitterion `CHEBI:144924`	MetaCyc:RXN-11115 Source assertion: The native right side explicitly contains CPD-12085 with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11115"]/right[/@frameid="CPD-12085"]; reaction-direction` MetaCyc:CPD-12085 Source assertion:* The original CPD-12085 structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:144924. Source location `ptools-xml/Compound[@frameid="CPD-12085"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:144924 Source assertion: CHEBI:144924 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:144924; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	produces	diphosphate(3-) `CHEBI:33019`	MetaCyc:RXN-11115 Source assertion: The native right side explicitly contains PPI with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11115"]/right[/@frameid="PPI"]; reaction-direction` MetaCyc:PPI Source assertion:* The original PPI structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:33019. Source location `ptools-xml/Compound[@frameid="PPI"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:33019 Source assertion: CHEBI:33019 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:33019; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
L-cysteinyl-AMP zwitterion `CHEBI:144924`	consumes	(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	MetaCyc:RXN-11117 Source assertion: The native left side explicitly contains CPD-12085 with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11117"]/left[/@frameid="CPD-12085"]; reaction-direction` MetaCyc:CPD-12085 Source assertion:* The original CPD-12085 structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:144924. Source location `ptools-xml/Compound[@frameid="CPD-12085"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:144924 Source assertion: CHEBI:144924 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:144924; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
a [PchF non-ribosomal peptide synthase protein] `MetaCyc:PchF`	consumes Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	MetaCyc:RXN-11117 Source assertion: The native left side explicitly contains PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11117"]/left[/@frameid="PchF"]; reaction-direction` MetaCyc:PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	produces Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	an L-cysteinyl-[PchF non-ribosomal peptide synthase] `MetaCyc:L-cysteinyl-PchF`	MetaCyc:RXN-11117 Source assertion: The native right side explicitly contains L-cysteinyl-PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11117"]/right[/@frameid="L-cysteinyl-PchF"]; reaction-direction` MetaCyc:L-cysteinyl-PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="L-cysteinyl-PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	produces	adenosine 5'-monophosphate(2-) `CHEBI:456215`	MetaCyc:RXN-11117 Source assertion: The native right side explicitly contains AMP with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11117"]/right[/@frameid="AMP"]; reaction-direction` MetaCyc:AMP Source assertion:* The original AMP structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:456215. Source location `ptools-xml/Compound[@frameid="AMP"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:456215 Source assertion: CHEBI:456215 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:456215; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	produces	hydron `CHEBI:15378`	MetaCyc:RXN-11117 Source assertion: The native right side explicitly contains PROTON with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11117"]/right[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] `MetaCyc:Salicyl-L-cysteinyl-PchE`	consumes Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] -> hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] + H2O `MetaCyc:RXN-11118`	MetaCyc:RXN-11118 Source assertion: The native left side explicitly contains Salicyl-L-cysteinyl-PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11118"]/left[/@frameid="Salicyl-L-cysteinyl-PchE"]; reaction-direction` MetaCyc:Salicyl-L-cysteinyl-PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="Salicyl-L-cysteinyl-PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] -> hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] + H2O `MetaCyc:RXN-11118`	produces Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] `MetaCyc:hydroxyphenyl-thiazolinyl-PchE`	MetaCyc:RXN-11118 Source assertion: The native right side explicitly contains hydroxyphenyl-thiazolinyl-PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11118"]/right[/@frameid="hydroxyphenyl-thiazolinyl-PchE"]; reaction-direction` MetaCyc:hydroxyphenyl-thiazolinyl-PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="hydroxyphenyl-thiazolinyl-PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] -> hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] + H2O `MetaCyc:RXN-11118`	produces	water `CHEBI:15377`	MetaCyc:RXN-11118 Source assertion: The native right side explicitly contains WATER with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11118"]/right[/@frameid="WATER"]; reaction-direction` MetaCyc:WATER Source assertion:* The original WATER structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15377. Source location `ptools-xml/Compound[@frameid="WATER"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15377 Source assertion: CHEBI:15377 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15377; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
an L-cysteinyl-[PchF non-ribosomal peptide synthase] `MetaCyc:L-cysteinyl-PchF`	consumes Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	MetaCyc:RXN-11120 Source assertion: The native left side explicitly contains L-cysteinyl-PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11120"]/left[/@frameid="L-cysteinyl-PchF"]; reaction-direction` MetaCyc:L-cysteinyl-PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="L-cysteinyl-PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] `MetaCyc:hydroxyphenyl-thiazolinyl-PchE`	consumes Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	MetaCyc:RXN-11120 Source assertion: The native left side explicitly contains hydroxyphenyl-thiazolinyl-PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11120"]/left[/@frameid="hydroxyphenyl-thiazolinyl-PchE"]; reaction-direction` MetaCyc:hydroxyphenyl-thiazolinyl-PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="hydroxyphenyl-thiazolinyl-PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	produces Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] `MetaCyc:Hydroxyphenyl-bis-thiazolinyl-PchF`	MetaCyc:RXN-11120 Source assertion: The native right side explicitly contains Hydroxyphenyl-bis-thiazolinyl-PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11120"]/right[/@frameid="Hydroxyphenyl-bis-thiazolinyl-PchF"]; reaction-direction` MetaCyc:Hydroxyphenyl-bis-thiazolinyl-PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="Hydroxyphenyl-bis-thiazolinyl-PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	produces	water `CHEBI:15377`	MetaCyc:RXN-11120 Source assertion: The native right side explicitly contains WATER with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11120"]/right[/@frameid="WATER"]; reaction-direction` MetaCyc:WATER Source assertion:* The original WATER structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15377. Source location `ptools-xml/Compound[@frameid="WATER"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15377 Source assertion: CHEBI:15377 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15377; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	produces Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	a [PchE non-ribosomal peptide synthase] `MetaCyc:PchE`	MetaCyc:RXN-11120 Source assertion: The native right side explicitly contains PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11120"]/right[/@frameid="PchE"]; reaction-direction` MetaCyc:PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	produces	hydron `CHEBI:15378`	MetaCyc:RXN-11120 Source assertion: The native right side explicitly contains PROTON with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11120"]/right[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] `MetaCyc:Hydroxyphenyl-bis-thiazolinyl-PchF`	consumes Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	MetaCyc:RXN-11121 Source assertion: The native left side explicitly contains Hydroxyphenyl-bis-thiazolinyl-PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11121"]/left[/@frameid="Hydroxyphenyl-bis-thiazolinyl-PchF"]; reaction-direction` MetaCyc:Hydroxyphenyl-bis-thiazolinyl-PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="Hydroxyphenyl-bis-thiazolinyl-PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
NADPH(4-) `CHEBI:57783`	consumes	hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	MetaCyc:RXN-11121 Source assertion: The native left side explicitly contains NADPH with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11121"]/left[/@frameid="NADPH"]; reaction-direction` MetaCyc:NADPH Source assertion:* The original NADPH structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:57783. Source location `ptools-xml/Compound[@frameid="NADPH"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:57783 Source assertion: CHEBI:57783 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:57783; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
hydron `CHEBI:15378`	consumes	hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	MetaCyc:RXN-11121 Source assertion: The native left side explicitly contains PROTON with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11121"]/left[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	produces Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] `MetaCyc:N-desmethyl-pyochelin-PchF`	MetaCyc:RXN-11121 Source assertion: The native right side explicitly contains N-desmethyl-pyochelin-PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11121"]/right[/@frameid="N-desmethyl-pyochelin-PchF"]; reaction-direction` MetaCyc:N-desmethyl-pyochelin-PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="N-desmethyl-pyochelin-PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	produces	NADP(3-) `CHEBI:58349`	MetaCyc:RXN-11121 Source assertion: The native right side explicitly contains NADP with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11121"]/right[/@frameid="NADP"]; reaction-direction` MetaCyc:NADP Source assertion:* The original NADP structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:58349. Source location `ptools-xml/Compound[@frameid="NADP"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:58349 Source assertion: CHEBI:58349 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:58349; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
S-adenosyl-L-methionine zwitterion `CHEBI:59789`	consumes	S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	MetaCyc:RXN-11122 Source assertion: The native left side explicitly contains S-ADENOSYLMETHIONINE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11122"]/left[/@frameid="S-ADENOSYLMETHIONINE"]; reaction-direction` MetaCyc:S-ADENOSYLMETHIONINE Source assertion:* The original S-ADENOSYLMETHIONINE structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:59789. Source location `ptools-xml/Compound[@frameid="S-ADENOSYLMETHIONINE"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:59789 Source assertion: CHEBI:59789 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:59789; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] `MetaCyc:N-desmethyl-pyochelin-PchF`	consumes Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	MetaCyc:RXN-11122 Source assertion: The native left side explicitly contains N-desmethyl-pyochelin-PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11122"]/left[/@frameid="N-desmethyl-pyochelin-PchF"]; reaction-direction` MetaCyc:N-desmethyl-pyochelin-PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="N-desmethyl-pyochelin-PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	produces Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	pyochelinyl-[PchF non-ribosomal peptide synthase] `MetaCyc:Pyochelinyl-PchF`	MetaCyc:RXN-11122 Source assertion: The native right side explicitly contains Pyochelinyl-PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11122"]/right[/@frameid="Pyochelinyl-PchF"]; reaction-direction` MetaCyc:Pyochelinyl-PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="Pyochelinyl-PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	produces	S-adenosyl-L-homocysteine zwitterion `CHEBI:57856`	MetaCyc:RXN-11122 Source assertion: The native right side explicitly contains ADENOSYL-HOMO-CYS with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11122"]/right[/@frameid="ADENOSYL-HOMO-CYS"]; reaction-direction` MetaCyc:ADENOSYL-HOMO-CYS Source assertion:* The original ADENOSYL-HOMO-CYS structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:57856. Source location `ptools-xml/Compound[@frameid="ADENOSYL-HOMO-CYS"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:57856 Source assertion: CHEBI:57856 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:57856; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	produces	hydron `CHEBI:15378`	MetaCyc:RXN-11122 Source assertion: The native right side explicitly contains PROTON with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11122"]/right[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
pyochelinyl-[PchF non-ribosomal peptide synthase] `MetaCyc:Pyochelinyl-PchF`	consumes Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	pyochelinyl-[PchF non-ribosomal peptide synthase] + H2O -> pyochelin + a [PchF non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-11123`	MetaCyc:RXN-11123 Source assertion: The native left side explicitly contains Pyochelinyl-PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11123"]/left[/@frameid="Pyochelinyl-PchF"]; reaction-direction` MetaCyc:Pyochelinyl-PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="Pyochelinyl-PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
water `CHEBI:15377`	consumes	pyochelinyl-[PchF non-ribosomal peptide synthase] + H2O -> pyochelin + a [PchF non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-11123`	MetaCyc:RXN-11123 Source assertion: The native left side explicitly contains WATER with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11123"]/left[/@frameid="WATER"]; reaction-direction` MetaCyc:WATER Source assertion:* The original WATER structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15377. Source location `ptools-xml/Compound[@frameid="WATER"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15377 Source assertion: CHEBI:15377 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15377; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
pyochelinyl-[PchF non-ribosomal peptide synthase] + H2O -> pyochelin + a [PchF non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-11123`	produces Formal native pyochelin I monoanion endpoint; no exclusive-I physiological product or I-selective release claim.	pyochelin `MetaCyc:CPD-9992`	MetaCyc:RXN-11123 Source assertion: The native right side explicitly contains CPD-9992 with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11123"]/right[/@frameid="CPD-9992"]; reaction-direction` MetaCyc:CPD-9992 Source assertion:* The issued CPD-9992 InChI has the monoanion layer /p-1 and the stereochemical layers /t9-,10+,13-/m1/s1. The structure alone does not establish physiological product ratios or enzyme release selectivity. Source location `ptools-xml/Compound[@frameid="CPD-9992"]/inchi; /p-1 and /t9-,10+,13-/m1/s1` CHEBI:67183 Source assertion: ChEBI names this neutral structure pyochelin I. Its connectivity and stereochemical InChI layers match native CPD-9992 after removing /p-1; this independently derived I assignment is protonation-normalized, not exact charge equivalence. Source location `/name; /chemical_data/charge; /default_structure/standard_inchi; explicit comparison with CPD-9992 /inchi after removal of /p-1` PMID:17938167 Source assertion: P. aeruginosa produces pyochelin I and II, differing at the interconverting thioaminal center; this does not establish I-selective release. Source location `responseWrapper/resultList/result[id="17938167"]/abstractText`
pyochelinyl-[PchF non-ribosomal peptide synthase] + H2O -> pyochelin + a [PchF non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-11123`	produces Native PchF reactive-domain state; coefficient is not an enzyme assembly count.	a [PchF non-ribosomal peptide synthase protein] `MetaCyc:PchF`	MetaCyc:RXN-11123 Source assertion: The native right side explicitly contains PchF with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11123"]/right[/@frameid="PchF"]; reaction-direction` MetaCyc:PchF Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="PchF"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
pyochelinyl-[PchF non-ribosomal peptide synthase] + H2O -> pyochelin + a [PchF non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-11123`	produces	hydron `CHEBI:15378`	MetaCyc:RXN-11123 Source assertion: The native right side explicitly contains PROTON with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11123"]/right[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
L-cysteinyl-AMP zwitterion `CHEBI:144924`	consumes	(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	MetaCyc:RXN-11125 Source assertion: The native left side explicitly contains CPD-12085 with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11125"]/left[/@frameid="CPD-12085"]; reaction-direction` MetaCyc:CPD-12085 Source assertion:* The original CPD-12085 structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:144924. Source location `ptools-xml/Compound[@frameid="CPD-12085"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:144924 Source assertion: CHEBI:144924 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:144924; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
a [PchE non-ribosomal peptide synthase] `MetaCyc:PchE`	consumes Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	MetaCyc:RXN-11125 Source assertion: The native left side explicitly contains PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11125"]/left[/@frameid="PchE"]; reaction-direction` MetaCyc:PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	produces Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	an L-cysteinyl-[PchE non-ribosomal peptide synthase] `MetaCyc:L-cysteinyl-PchE`	MetaCyc:RXN-11125 Source assertion: The native right side explicitly contains L-cysteinyl-PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11125"]/right[/@frameid="L-cysteinyl-PchE"]; reaction-direction` MetaCyc:L-cysteinyl-PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="L-cysteinyl-PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	produces	adenosine 5'-monophosphate(2-) `CHEBI:456215`	MetaCyc:RXN-11125 Source assertion: The native right side explicitly contains AMP with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11125"]/right[/@frameid="AMP"]; reaction-direction` MetaCyc:AMP Source assertion:* The original AMP structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:456215. Source location `ptools-xml/Compound[@frameid="AMP"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:456215 Source assertion: CHEBI:456215 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:456215; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	produces	hydron `CHEBI:15378`	MetaCyc:RXN-11125 Source assertion: The native right side explicitly contains PROTON with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-11125"]/right[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
2-hydroxybenzoyl-AMP(1-) `CHEBI:86458`	consumes	(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	MetaCyc:RXN-19466 Source assertion: The native left side explicitly contains CPD-12084 with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19466"]/left[/@frameid="CPD-12084"]; reaction-direction` MetaCyc:CPD-12084 Source assertion:* The original CPD-12084 structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:86458. Source location `ptools-xml/Compound[@frameid="CPD-12084"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:86458 Source assertion: CHEBI:86458 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:86458; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
a [PchE non-ribosomal peptide synthase] `MetaCyc:PchE`	consumes Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	MetaCyc:RXN-19466 Source assertion: The native left side explicitly contains PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19466"]/left[/@frameid="PchE"]; reaction-direction` MetaCyc:PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	produces Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	a salicyl-[PchE non-ribosomal peptide synthase] `MetaCyc:Salicyl-PchE`	MetaCyc:RXN-19466 Source assertion: The native right side explicitly contains Salicyl-PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19466"]/right[/@frameid="Salicyl-PchE"]; reaction-direction` MetaCyc:Salicyl-PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="Salicyl-PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	produces	adenosine 5'-monophosphate(2-) `CHEBI:456215`	MetaCyc:RXN-19466 Source assertion: The native right side explicitly contains AMP with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19466"]/right[/@frameid="AMP"]; reaction-direction` MetaCyc:AMP Source assertion:* The original AMP structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:456215. Source location `ptools-xml/Compound[@frameid="AMP"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:456215 Source assertion: CHEBI:456215 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:456215; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	produces	hydron `CHEBI:15378`	MetaCyc:RXN-19466 Source assertion: The native right side explicitly contains PROTON with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19466"]/right[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
a salicyl-[PchE non-ribosomal peptide synthase] `MetaCyc:Salicyl-PchE`	consumes Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	MetaCyc:RXN-19469 Source assertion: The native left side explicitly contains Salicyl-PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19469"]/left[/@frameid="Salicyl-PchE"]; reaction-direction` MetaCyc:Salicyl-PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="Salicyl-PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
an L-cysteinyl-[PchE non-ribosomal peptide synthase] `MetaCyc:L-cysteinyl-PchE`	consumes Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	MetaCyc:RXN-19469 Source assertion: The native left side explicitly contains L-cysteinyl-PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19469"]/left[/@frameid="L-cysteinyl-PchE"]; reaction-direction` MetaCyc:L-cysteinyl-PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="L-cysteinyl-PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	produces Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] `MetaCyc:Salicyl-L-cysteinyl-PchE`	MetaCyc:RXN-19469 Source assertion: The native right side explicitly contains Salicyl-L-cysteinyl-PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19469"]/right[/@frameid="Salicyl-L-cysteinyl-PchE"]; reaction-direction` MetaCyc:Salicyl-L-cysteinyl-PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="Salicyl-L-cysteinyl-PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	produces Native reactive-domain class; PchE aryl and peptidyl sites are separate reaction sites on the same polypeptide.	a [PchE non-ribosomal peptide synthase] `MetaCyc:PchE`	MetaCyc:RXN-19469 Source assertion: The native right side explicitly contains PchE with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19469"]/right[/@frameid="PchE"]; reaction-direction` MetaCyc:PchE Source assertion:* The original frame explicitly declares a Protein class and names its carrier acyl state; it supplies no full protein sequence or atom-complete carrier structure. Source location `ptools-xml/Protein[@frameid="PchE"]/common-name; @class` DOI:10.1038/s41467-022-28284-x Source assertion: The original assembly diagram distinguishes the PchE ArCP and PCP sites and the PchF carrier site with covalent phosphopantetheine-tethered intermediates. Reactive-site accounting must not be read as whole-polypeptide coefficients. Source location `Original supplement PDF page2, Supplementary Figure1c; page3, Supplementary Figure2`
a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	produces	hydron `CHEBI:15378`	MetaCyc:RXN-19469 Source assertion: The native right side explicitly contains PROTON with coefficient 1; direction is PHYSIOL-LEFT-TO-RIGHT. Source location `ptools-xml/Reaction[@frameid="RXN-19469"]/right[/@frameid="PROTON"]; reaction-direction` MetaCyc:PROTON Source assertion:* The original PROTON structure defines the chemical form selected for this side; its InChI and charged atoms were compared with CHEBI:15378. Source location `ptools-xml/Compound[@frameid="PROTON"]/inchi; cml/molecule/atomArray/atom/@formalCharge` CHEBI:15378 Source assertion: CHEBI:15378 supplies the matching structure, formula and charge. Zwitterionic forms preserve the native amino/carboxyl charges where present. Source location `Semantic SQL statements subject=CHEBI:15378; chemrof:inchi_string, chemrof:charge, chemrof:generalized_empirical_formula`
Pyochelin synthase PchD `UniProtKB:Q9HWG3`	enables PchD adenylates salicylate before transfer to the PchE aryl carrier site. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	salicylate + ATP + H+ -> (salicyl)adenylate + diphosphate `MetaCyc:RXN-11113`	UniProtKB:Q9HWG3 Source assertion: Current PAO1 identity and functional annotation support this gene-product role. Detailed PchD and PchE/F steps include ECO:0000250 or ECO:0000305 inference; this is not a claim of purified monomer sufficiency. Source location `/results/3/primaryAccession; /results/3/organism/taxonId; /results/3/comments/0; /results/3/sequence/value` PMID:35105906 Source assertion: PchD adenylates salicylate before transfer to the PchE aryl carrier site. The inspected work uses PAO1 genes and observes dimeric PchE; the functional relation does not assert a stable four-protein complex. Source location `article/body//sec[@id="Sec1"]; sec[@id="Sec4"] and sec[@id="Sec5"]; Methods article/body//sec[@id="Sec9"]`
Pyochelin synthetase PchE `UniProtKB:G3XCV2`	enables The PchE and PchF adenylation domains each activate a cysteine; one native reaction object is reused twice. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	UniProtKB:G3XCV2 Source assertion: Current PAO1 identity and functional annotation support this gene-product role. Detailed PchD and PchE/F steps include ECO:0000250 or ECO:0000305 inference; this is not a claim of purified monomer sufficiency. Source location `/results/1/primaryAccession; /results/1/organism/taxonId; /results/1/comments/0; /results/1/sequence/value` PMID:35105906 Source assertion: The PchE and PchF adenylation domains each activate a cysteine; one native reaction object is reused twice. The inspected work uses PAO1 genes and observes dimeric PchE; the functional relation does not assert a stable four-protein complex. Source location `article/body//sec[@id="Sec1"]; sec[@id="Sec4"] and sec[@id="Sec5"]; Methods article/body//sec[@id="Sec9"]`
Pyochelin synthetase PchF `UniProtKB:Q9HWG4`	enables The PchE and PchF adenylation domains each activate a cysteine; one native reaction object is reused twice. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	UniProtKB:Q9HWG4 Source assertion: Current PAO1 identity and functional annotation support this gene-product role. Detailed PchD and PchE/F steps include ECO:0000250 or ECO:0000305 inference; this is not a claim of purified monomer sufficiency. Source location `/results/2/primaryAccession; /results/2/organism/taxonId; /results/2/comments/0; /results/2/sequence/value` PMID:30525512 Source assertion: The PchE and PchF adenylation domains each activate a cysteine; one native reaction object is reused twice. The study names O85740 and uses PAO1 constructs; detailed methylation tests use analogues, not complete thiotemplate steady-state turnover. Source location `collection/document/passage[offset="9139" or offset="9446" or offset="41012" or offset="53276" or offset="65484"]/text` PMID:11467965 Source assertion: Four-protein reconstitution assigns terminal reduction to PchG and methylation/release to PchF. Only the original indexed abstract was retained; no fixed DEFG heterotetramer follows. Source location `responseWrapper/resultList/result[id="11467965"]/abstractText`
Pyochelin synthetase PchF `UniProtKB:Q9HWG4`	enables PchF loads its activated cysteine onto its carrier site. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	UniProtKB:Q9HWG4 Source assertion: Current PAO1 identity and functional annotation support this gene-product role. Detailed PchD and PchE/F steps include ECO:0000250 or ECO:0000305 inference; this is not a claim of purified monomer sufficiency. Source location `/results/2/primaryAccession; /results/2/organism/taxonId; /results/2/comments/0; /results/2/sequence/value` PMID:30525512 Source assertion: PchF loads its activated cysteine onto its carrier site. The study names O85740 and uses PAO1 constructs; detailed methylation tests use analogues, not complete thiotemplate steady-state turnover. Source location `collection/document/passage[offset="9139" or offset="9446" or offset="41012" or offset="53276" or offset="65484"]/text` PMID:11467965 Source assertion: Four-protein reconstitution assigns terminal reduction to PchG and methylation/release to PchF. Only the original indexed abstract was retained; no fixed DEFG heterotetramer follows. Source location `responseWrapper/resultList/result[id="11467965"]/abstractText`
Pyochelin synthetase PchE `UniProtKB:G3XCV2`	enables PchE performs first-ring cyclization with epimerization after amide formation. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] -> hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] + H2O `MetaCyc:RXN-11118`	UniProtKB:G3XCV2 Source assertion: Current PAO1 identity and functional annotation support this gene-product role. Detailed PchD and PchE/F steps include ECO:0000250 or ECO:0000305 inference; this is not a claim of purified monomer sufficiency. Source location `/results/1/primaryAccession; /results/1/organism/taxonId; /results/1/comments/0; /results/1/sequence/value` PMID:35105906 Source assertion: PchE performs first-ring cyclization with epimerization after amide formation. The inspected work uses PAO1 genes and observes dimeric PchE; the functional relation does not assert a stable four-protein complex. Source location `article/body//sec[@id="Sec1"]; sec[@id="Sec4"] and sec[@id="Sec5"]; Methods article/body//sec[@id="Sec9"]` PMID:12950179 Source assertion: Epimerization was detected after amide formation rather than on simple cysteinyl-S-PchE; the native ring-forming object compresses these coupled events. Source location `responseWrapper/resultList/result[id="12950179"]/abstractText`
Pyochelin synthetase PchF `UniProtKB:Q9HWG4`	enables PchF accepts the PchE-bound intermediate, condenses the second cysteine and forms the second thiazoline. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	UniProtKB:Q9HWG4 Source assertion: Current PAO1 identity and functional annotation support this gene-product role. Detailed PchD and PchE/F steps include ECO:0000250 or ECO:0000305 inference; this is not a claim of purified monomer sufficiency. Source location `/results/2/primaryAccession; /results/2/organism/taxonId; /results/2/comments/0; /results/2/sequence/value` PMID:30525512 Source assertion: PchF accepts the PchE-bound intermediate, condenses the second cysteine and forms the second thiazoline. The study names O85740 and uses PAO1 constructs; detailed methylation tests use analogues, not complete thiotemplate steady-state turnover. Source location `collection/document/passage[offset="9139" or offset="9446" or offset="41012" or offset="53276" or offset="65484"]/text` PMID:11467965 Source assertion: Four-protein reconstitution assigns terminal reduction to PchG and methylation/release to PchF. Only the original indexed abstract was retained; no fixed DEFG heterotetramer follows. Source location `responseWrapper/resultList/result[id="11467965"]/abstractText`
Pyochelin biosynthetic protein PchG `UniProtKB:Q9HWG5`	enables PchG reduces the PchF-tethered terminal thiazoline using NADPH. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	UniProtKB:Q9HWG5 Source assertion: The current unreviewed PAO1 entry identifies PchG; the biochemical assignment is supported separately by original PAO1 evidence. Source location `/results/5/primaryAccession; /results/5/organism/taxonId; /results/5/proteinDescription; /results/5/sequence/value` PMID:11467965 Source assertion: Purified PchG reduces the hydroxyphenylbisthiazoline-S-PchF acyl enzyme with NADPH during complete reconstitution. Only the original indexed abstract was retained; historical construct scope is distinct from exact current PAO1 sequence identity. Source location `responseWrapper/resultList/result[id="11467965"]/abstractText` PMID:11208777 Source assertion: P. aeruginosa genetics and biochemical reconstitution establish the PchG requirement for pyochelin synthesis, separately from the current unreviewed sequence annotation. Source location `responseWrapper/resultList/result[id="11208777"]/abstractText` PMID:27601130 Source assertion: PAO1-derived PchG reduces an oxidized free analogue with NADPH; the authors caution that this is a poor model for full physiological carrier-substrate kinetics and stereochemistry. Source location `collection/document/passage[offset="4948" or offset="34049"]/text; Results, Enzymatic Activity`
Pyochelin synthetase PchF `UniProtKB:Q9HWG4`	enables PchF methylates the reduced terminal nitrogen with SAM. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	UniProtKB:Q9HWG4 Source assertion: The current entry identifies Q9HWG4 as PAO1 PchF and supplies its sequence. This identity evidence does not independently assign methylation or mature-product hydrolysis; those roles are supported by separate primary evidence. Source location `/results/2/primaryAccession; /results/2/genes/0/geneName/value; /results/2/organism/taxonId; /results/2/sequence/value` PMID:30525512 Source assertion: PchF methylates the reduced terminal nitrogen with SAM. The study names O85740 and uses PAO1 constructs; detailed methylation tests use analogues, not complete thiotemplate steady-state turnover. Source location `collection/document/passage[offset="9139" or offset="9446" or offset="41012" or offset="53276" or offset="65484"]/text` PMID:11467965 Source assertion: Four-protein reconstitution assigns terminal reduction to PchG and methylation/release to PchF. Only the original indexed abstract was retained; no fixed DEFG heterotetramer follows. Source location `responseWrapper/resultList/result[id="11467965"]/abstractText`
Pyochelin synthetase PchF `UniProtKB:Q9HWG4`	enables PchF releases pyochelin by thioester hydrolysis; the native I structure is a formal projection of the observed I/II products. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	pyochelinyl-[PchF non-ribosomal peptide synthase] + H2O -> pyochelin + a [PchF non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-11123`	UniProtKB:Q9HWG4 Source assertion: The current entry identifies Q9HWG4 as PAO1 PchF and supplies its sequence. This identity evidence does not independently assign methylation or mature-product hydrolysis; those roles are supported by separate primary evidence. Source location `/results/2/primaryAccession; /results/2/genes/0/geneName/value; /results/2/organism/taxonId; /results/2/sequence/value` PMID:30525512 Source assertion: PchF releases pyochelin by thioester hydrolysis. The study names O85740 and uses PAO1 constructs; detailed methylation tests use analogues, not complete thiotemplate steady-state turnover. Source location `collection/document/passage[offset="9139" or offset="9446" or offset="41012" or offset="53276" or offset="65484"]/text` PMID:11467965 Source assertion: Four-protein reconstitution assigns terminal reduction to PchG and methylation/release to PchF. Only the original indexed abstract was retained; no fixed DEFG heterotetramer follows. Source location `responseWrapper/resultList/result[id="11467965"]/abstractText`
Pyochelin synthetase PchE `UniProtKB:G3XCV2`	enables PchE loads cysteine onto its peptidyl carrier site. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	UniProtKB:G3XCV2 Source assertion: Current PAO1 identity and functional annotation support this gene-product role. Detailed PchD and PchE/F steps include ECO:0000250 or ECO:0000305 inference; this is not a claim of purified monomer sufficiency. Source location `/results/1/primaryAccession; /results/1/organism/taxonId; /results/1/comments/0; /results/1/sequence/value` PMID:35105906 Source assertion: PchE loads cysteine onto its peptidyl carrier site. The inspected work uses PAO1 genes and observes dimeric PchE; the functional relation does not assert a stable four-protein complex. Source location `article/body//sec[@id="Sec1"]; sec[@id="Sec4"] and sec[@id="Sec5"]; Methods article/body//sec[@id="Sec9"]`
Pyochelin synthase PchD `UniProtKB:Q9HWG3`	enables PchD transfers activated salicylate to the PchE aryl carrier site. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	UniProtKB:Q9HWG3 Source assertion: Current PAO1 identity and functional annotation support this gene-product role. Detailed PchD and PchE/F steps include ECO:0000250 or ECO:0000305 inference; this is not a claim of purified monomer sufficiency. Source location `/results/3/primaryAccession; /results/3/organism/taxonId; /results/3/comments/0; /results/3/sequence/value` PMID:35105906 Source assertion: PchD transfers activated salicylate to the PchE aryl carrier site. The inspected work uses PAO1 genes and observes dimeric PchE; the functional relation does not assert a stable four-protein complex. Source location `article/body//sec[@id="Sec1"]; sec[@id="Sec4"] and sec[@id="Sec5"]; Methods article/body//sec[@id="Sec9"]`
Pyochelin synthetase PchE `UniProtKB:G3XCV2`	enables PchE condenses its aryl-site salicyl group with its peptidyl-site cysteinyl group. Functional gene-product relation; no monomer sufficiency or stable PchDEFG assembly is asserted.	a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	UniProtKB:G3XCV2 Source assertion: Current PAO1 identity and functional annotation support this gene-product role. Detailed PchD and PchE/F steps include ECO:0000250 or ECO:0000305 inference; this is not a claim of purified monomer sufficiency. Source location `/results/1/primaryAccession; /results/1/organism/taxonId; /results/1/comments/0; /results/1/sequence/value` PMID:35105906 Source assertion: PchE condenses its aryl-site salicyl group with its peptidyl-site cysteinyl group. The inspected work uses PAO1 genes and observes dimeric PchE; the functional relation does not assert a stable four-protein complex. Source location `article/body//sec[@id="Sec1"]; sec[@id="Sec4"] and sec[@id="Sec5"]; Methods article/body//sec[@id="Sec9"]`
Pyochelin synthetase PchE `UniProtKB:G3XCV2`	has_cofactor Covalently attached carrier-arm moiety; no free-cofactor consumption or inferred whole-protein count.	pantetheine 4'-phosphate(2-) `CHEBI:47942`	UniProtKB:G3XCV2 Source assertion: The entry assigns pantetheine 4'-phosphate as a cofactor with ECO:0000305. In this pathway its covalently attached arm carries intermediates; it is not a free stoichiometric substrate. Source location `/results/1/comments/2/cofactors/0; cofactorCrossReference/id=CHEBI:47942`
Pyochelin synthetase PchF `UniProtKB:Q9HWG4`	has_cofactor Covalently attached carrier-arm moiety; no free-cofactor consumption or inferred whole-protein count.	pantetheine 4'-phosphate(2-) `CHEBI:47942`	UniProtKB:Q9HWG4 Source assertion: The entry assigns pantetheine 4'-phosphate as a cofactor with ECO:0000305. In this pathway its covalently attached arm carries intermediates; it is not a free stoichiometric substrate. Source location `/results/2/comments/2/cofactors/0; cofactorCrossReference/id=CHEBI:47942`
Pyochelin synthetase PchE `UniProtKB:G3XCV2`	has_cofactor Direct bound-metal observation in the PAO1 PchE construct; no transfer of an MgATP assay condition to other proteins.	magnesium(2+) `CHEBI:18420`	PMID:35105906 Source assertion: Cryo-EM structures directly place Mg2+ with cysteinyl-AMP in the PchE A-domain pocket. This supports bound magnesium in the inspected PAO1 construct, without a universal metal-dependence or stoichiometric claim. Source location `article/body//sec[@id="Sec5"]/p describing cysteinyl-AMP and Mg2+; Figure 3b`
salicylate + ATP + H+ -> (salicyl)adenylate + diphosphate `MetaCyc:RXN-11113`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-11113; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-11113"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-11115; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-11115"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-11117; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-11117"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] -> hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] + H2O `MetaCyc:RXN-11118`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-11118; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-11118"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-11120; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-11120"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-11121; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-11121"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-11122; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-11122"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
pyochelinyl-[PchF non-ribosomal peptide synthase] + H2O -> pyochelin + a [PchF non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-11123`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-11123; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-11123"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-11125; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-11125"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-19466; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-19466"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	part_of	`GO:0042864`	MetaCyc:PWY-6408 Source assertion: The complete native reaction-list contains RXN-19469; the selected scope begins at salicylate and retains all eleven listed reaction objects. Source location `ptools-xml/Pathway[@frameid="PWY-6408"]/reaction-list/Reaction[@frameid="RXN-19469"]` GO:0042864 Source assertion: The active GO process denotes chemical reactions and pathways forming pyochelin; this record describes the complete native salicylate-dependent implementation. Source location `/results/0/id; /results/0/isObsolete; /results/0/definition/text`
salicylate + ATP + H+ -> (salicyl)adenylate + diphosphate `MetaCyc:RXN-11113`	provides_input_for	(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	MetaCyc:RXN-11113 Source assertion: The original product side contains CPD-12084; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-11113"]/right[/@frameid="CPD-12084"]` MetaCyc:RXN-19466 Source assertion:* The original substrate side contains CPD-12084; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-19466"]/left[*/@frameid="CPD-12084"]`
L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	provides_input_for	(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	MetaCyc:RXN-11115 Source assertion: The original product side contains CPD-12085; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-11115"]/right[/@frameid="CPD-12085"]` MetaCyc:RXN-11125 Source assertion:* The original substrate side contains CPD-12085; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-11125"]/left[*/@frameid="CPD-12085"]`
L-cysteine + ATP + H+ -> (L-cysteinyl)adenylate + diphosphate `MetaCyc:RXN-11115`	provides_input_for	(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	MetaCyc:RXN-11115 Source assertion: The original product side contains CPD-12085; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-11115"]/right[/@frameid="CPD-12085"]` MetaCyc:RXN-11117 Source assertion:* The original substrate side contains CPD-12085; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-11117"]/left[*/@frameid="CPD-12085"]`
(salicyl)adenylate + a [PchE non-ribosomal peptide synthase] -> a salicyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-19466`	provides_input_for	a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	MetaCyc:RXN-19466 Source assertion: The original product side contains Salicyl-PchE; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-19466"]/right[/@frameid="Salicyl-PchE"]` MetaCyc:RXN-19469 Source assertion:* The original substrate side contains Salicyl-PchE; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-19469"]/left[*/@frameid="Salicyl-PchE"]`
(L-cysteinyl)adenylate + a [PchE non-ribosomal peptide synthase] -> an L-cysteinyl-[PchE non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11125`	provides_input_for	a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	MetaCyc:RXN-11125 Source assertion: The original product side contains L-cysteinyl-PchE; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-11125"]/right[/@frameid="L-cysteinyl-PchE"]` MetaCyc:RXN-19469 Source assertion:* The original substrate side contains L-cysteinyl-PchE; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-19469"]/left[*/@frameid="L-cysteinyl-PchE"]`
a salicyl-[PchE non-ribosomal peptide synthase] + an L-cysteinyl-[PchE non-ribosomal peptide synthase] -> salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] + a [PchE non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-19469`	provides_input_for	salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] -> hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] + H2O `MetaCyc:RXN-11118`	MetaCyc:RXN-19469 Source assertion: The original product side contains Salicyl-L-cysteinyl-PchE; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-19469"]/right[/@frameid="Salicyl-L-cysteinyl-PchE"]` MetaCyc:RXN-11118 Source assertion:* The original substrate side contains Salicyl-L-cysteinyl-PchE; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-11118"]/left[*/@frameid="Salicyl-L-cysteinyl-PchE"]`
salicyl-L-cysteinyl-[PchE non-ribosomal peptide synthas] -> hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] + H2O `MetaCyc:RXN-11118`	provides_input_for	an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	MetaCyc:RXN-11118 Source assertion: The original product side contains hydroxyphenyl-thiazolinyl-PchE; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-11118"]/right[/@frameid="hydroxyphenyl-thiazolinyl-PchE"]` MetaCyc:RXN-11120 Source assertion:* The original substrate side contains hydroxyphenyl-thiazolinyl-PchE; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-11120"]/left[*/@frameid="hydroxyphenyl-thiazolinyl-PchE"]`
(L-cysteinyl)adenylate + a [PchF non-ribosomal peptide synthase] -> an L-cysteinyl-[PchF non-ribosomal peptide synthase] + AMP + H+ `MetaCyc:RXN-11117`	provides_input_for	an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	MetaCyc:RXN-11117 Source assertion: The original product side contains L-cysteinyl-PchF; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-11117"]/right[/@frameid="L-cysteinyl-PchF"]` MetaCyc:RXN-11120 Source assertion:* The original substrate side contains L-cysteinyl-PchF; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-11120"]/left[*/@frameid="L-cysteinyl-PchF"]`
an L-cysteinyl-[PchF non-ribosomal peptide synthase] + hydroxyphenyl-thiazolinyl-[PchE non-ribosomal peptide synthase] -> hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + a [PchE non-ribosomal peptide synthase] + H2O + H+ `MetaCyc:RXN-11120`	provides_input_for	hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	MetaCyc:RXN-11120 Source assertion: The original product side contains Hydroxyphenyl-bis-thiazolinyl-PchF; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-11120"]/right[/@frameid="Hydroxyphenyl-bis-thiazolinyl-PchF"]` MetaCyc:RXN-11121 Source assertion:* The original substrate side contains Hydroxyphenyl-bis-thiazolinyl-PchF; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-11121"]/left[*/@frameid="Hydroxyphenyl-bis-thiazolinyl-PchF"]`
hydroxyphenyl-4,2-bis-thiazolinyl-[PchF non-ribosomal peptide synthase] + NADPH + H+ -> N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] + NADP+ `MetaCyc:RXN-11121`	provides_input_for	S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	MetaCyc:RXN-11121 Source assertion: The original product side contains N-desmethyl-pyochelin-PchF; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-11121"]/right[/@frameid="N-desmethyl-pyochelin-PchF"]` MetaCyc:RXN-11122 Source assertion:* The original substrate side contains N-desmethyl-pyochelin-PchF; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-11122"]/left[*/@frameid="N-desmethyl-pyochelin-PchF"]`
S-adenosyl-L-methionine + N-desmethyl-pyochelin-[PchF non-ribosomal peptide synthase] -> pyochelinyl-[PchF non-ribosomal peptide synthase] + S-adenosyl-L-homocysteine + H+ `MetaCyc:RXN-11122`	provides_input_for	pyochelinyl-[PchF non-ribosomal peptide synthase] + H2O -> pyochelin + a [PchF non-ribosomal peptide synthase] + H+ `MetaCyc:RXN-11123`	MetaCyc:RXN-11122 Source assertion: The original product side contains Pyochelinyl-PchF; the subsequent reaction consumes that same source-native intermediate. Source location `ptools-xml/Reaction[@frameid="RXN-11122"]/right[/@frameid="Pyochelinyl-PchF"]` MetaCyc:RXN-11123 Source assertion:* The original substrate side contains Pyochelinyl-PchF; this supplies the second half of the intermediate bridge. Source location `ptools-xml/Reaction[@frameid="RXN-11123"]/left[*/@frameid="Pyochelinyl-PchF"]`

References

MetaCyc:ADENOSYL-HOMO-CYS MetaCyc ADENOSYL-HOMO-CYS original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:AMP MetaCyc AMP original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:ATP MetaCyc ATP original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:CPD-110 MetaCyc CPD-110 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:CPD-12084 MetaCyc CPD-12084 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:CPD-12085 MetaCyc CPD-12085 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:CPD-9992 MetaCyc CPD-9992 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:CYS MetaCyc CYS original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:Hydroxyphenyl-bis-thiazolinyl-PchF MetaCyc Hydroxyphenyl-bis-thiazolinyl-PchF original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:L-cysteinyl-PchE MetaCyc L-cysteinyl-PchE original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:L-cysteinyl-PchF MetaCyc L-cysteinyl-PchF original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:N-desmethyl-pyochelin-PchF MetaCyc N-desmethyl-pyochelin-PchF original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:NADP MetaCyc NADP original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:NADPH MetaCyc NADPH original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:PPI MetaCyc PPI original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:PROTON MetaCyc PROTON original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:PchE MetaCyc PchE original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:PchF MetaCyc PchF original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:Pyochelinyl-PchF MetaCyc Pyochelinyl-PchF original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:S-ADENOSYLMETHIONINE MetaCyc S-ADENOSYLMETHIONINE original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:Salicyl-L-cysteinyl-PchE MetaCyc Salicyl-L-cysteinyl-PchE original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:Salicyl-PchE MetaCyc Salicyl-PchE original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:WATER MetaCyc WATER original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:hydroxyphenyl-thiazolinyl-PchE MetaCyc hydroxyphenyl-thiazolinyl-PchE original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-11113 MetaCyc RXN-11113 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-11115 MetaCyc RXN-11115 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-11117 MetaCyc RXN-11117 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-11118 MetaCyc RXN-11118 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-11120 MetaCyc RXN-11120 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-11121 MetaCyc RXN-11121 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-11122 MetaCyc RXN-11122 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-11123 MetaCyc RXN-11123 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-11125 MetaCyc RXN-11125 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-19466 MetaCyc RXN-19466 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:RXN-19469 MetaCyc RXN-19469 original native frame (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
MetaCyc:PWY-6408 MetaCyc PWY-6408 pyochelin biosynthesis original pathway (source version: Original MetaCyc 22.5 native XML; retrieved 2026-10-11; digest covers complete original response)
GO:0042864 Gene Ontology GO:0042864 pyochelin biosynthetic process (source version: Original QuickGO term response retrieved 2026-10-11; active exact process identity)
CHEBI:144924 ChEBI 255 CHEBI:144924 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:15377 ChEBI 255 CHEBI:15377 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:15378 ChEBI 255 CHEBI:15378 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:30616 ChEBI 255 CHEBI:30616 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:30762 ChEBI 255 CHEBI:30762 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:33019 ChEBI 255 CHEBI:33019 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:35235 ChEBI 255 CHEBI:35235 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:456215 ChEBI 255 CHEBI:456215 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:57783 ChEBI 255 CHEBI:57783 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:57856 ChEBI 255 CHEBI:57856 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:58349 ChEBI 255 CHEBI:58349 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:59789 ChEBI 255 CHEBI:59789 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
CHEBI:86458 ChEBI 255 CHEBI:86458 original chemical structure and preferred name (source version: ChEBI 255 Semantic SQL; digest covers the complete uncompressed original authority database)
UniProtKB:Q9HWG3 UniProtKB Q9HWG3 current complete PAO1 entry (source version: UniProt 2026_03; complete original seven-entry response retrieved 2026-10-11; entry 3; digest covers whole batch)
UniProtKB:G3XCV2 UniProtKB G3XCV2 current complete PAO1 entry (source version: UniProt 2026_03; complete original seven-entry response retrieved 2026-10-11; entry 1; digest covers whole batch)
UniProtKB:Q9HWG4 UniProtKB Q9HWG4 current complete PAO1 entry (source version: UniProt 2026_03; complete original seven-entry response retrieved 2026-10-11; entry 2; digest covers whole batch)
UniProtKB:Q9HWG5 UniProtKB Q9HWG5 current complete PAO1 entry (source version: UniProt 2026_03; complete original seven-entry response retrieved 2026-10-11; entry 5; digest covers whole batch)
PMID:17938167 Pseudomonas fluorescens CHA0 produces enantio-pyochelin, the optical antipode of the Pseudomonas aeruginosa siderophore pyochelin. (source version: Original Europe PMC indexed primary metadata and abstract; full article not locally obtained; digest covers complete response)
PMID:15375116 PchC thioesterase optimizes nonribosomal biosynthesis of the peptide siderophore pyochelin in Pseudomonas aeruginosa. (source version: Original Europe PMC indexed primary metadata and abstract; full article not locally obtained; digest covers complete response)
PMID:11467965 In vitro reconstitution of the Pseudomonas aeruginosa nonribosomal peptide synthesis of pyochelin: characterization of backbone tailoring thiazoline reductase and N-methyltransferase activities. (source version: Original Europe PMC indexed primary metadata and abstract; full article not locally obtained; digest covers complete response)
PMID:10555976 Assembly of the Pseudomonas aeruginosa nonribosomal peptide siderophore pyochelin: In vitro reconstitution of aryl-4, 2-bisthiazoline synthetase activity from PchD, PchE, and PchF. (source version: Original Europe PMC indexed primary metadata and abstract; full article not locally obtained; digest covers complete response)
PMID:12950179 Epimerization of an L-cysteinyl to a D-cysteinyl residue during thiazoline ring formation in siderophore chain elongation by pyochelin synthetase from Pseudomonas aeruginosa. (source version: Original Europe PMC indexed primary metadata and abstract; full article not locally obtained; digest covers complete response)
PMID:11208777 Essential PchG-dependent reduction in pyochelin biosynthesis of Pseudomonas aeruginosa. (source version: Original Europe PMC indexed primary metadata and abstract; full article not locally obtained; digest covers complete response)
PMID:9846750 Dihydroaeruginoic acid synthetase and pyochelin synthetase, products of the pchEF genes, are induced by extracellular pyochelin in Pseudomonas aeruginosa. (source version: Original Europe PMC indexed primary metadata and abstract; full article not locally obtained; digest covers complete response)
PMID:35105906 Catalytic trajectory of a dimeric nonribosomal peptide synthetase subunit with an inserted epimerase domain (source version: Complete original Europe PMC JATS article XML retrieved 2026-10-11; digest covers whole response)
PMID:30525512 Stuffed Methyltransferase Catalyzes Penultimate Step of Pyochelin Biosynthesis (source version: Complete original NCBI BioC author-manuscript XML export retrieved 2026-10-11; digest covers whole response)
PMID:27601130 Holo Structure and Steady State Kinetics of the Thiazolinyl Imine Reductases for Siderophore Biosynthesis (source version: Complete original NCBI BioC author-manuscript XML export retrieved 2026-10-11; digest covers whole response)
DOI:10.1038/s41467-022-28284-x Supplementary Information for Catalytic trajectory of a dimeric nonribosomal peptide synthetase subunit with an inserted epimerase domain (source version: Original 27-page publisher supplement; Figure1c and Figure2 inspected; digest covers exact PDF bytes, not the main article)
NCBIProtein:AAC83656.1 Original PAO1 AF074705.2 submission containing PchE, PchF and PchG CDS translations (source version: Original ENA EMBL record; digest covers AF074705.2 complete response, not only one protein)
CHEBI:67183 ChEBI pyochelin I original compound JSON (source version: Original ChEBI compound API response retrieved 2026-10-11; digest covers complete JSON response, neutral structure)

Curation history

2026-10-11T16:03:17Z codex
- CREATE — Add the complete native pyochelin assembly pathway with source-specific evidence
  Add GO:0042864 covering all eleven native PWY-6408 transformations from supplied salicylate to released pyochelin, with30participants,91edges and242evidence blocks. Preserve reactive-domain accounting, native I monoanion versus observed I/II limits, source-strain and construct distinctions, and analogy/inference qualifiers. Current b3dfb5d record includes exactly five independently verified source-local corrections from external issue397; original c72de0b target, initial observations and exact successors remain in the cohort216 research dossier. External independent review and fresh root95-original-body verification passed; canonical maintained review and full corpus gates follow separately.
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