carbon fixation by 3-hydroxypropionate cycle

GO:0043427

The complete first 3-hydroxypropionate cycle fixes two inorganic-carbon units into glyoxylate while regenerating acetyl-CoA. It includes all 13 objects listed in native MetaCyc PWY-5743 plus experimentally supported succinate oxidation and fumarate hydration omitted from that list. CO2/bicarbonate equilibration and the two protein-bound biotin half-reactions remain explicit; there is no duplicated overall acetyl-CoA carboxylation. The distinct second cycle that assimilates glyoxylate into pyruvate is outside this GO boundary. The primary whole-cycle experiments used Chloroflexus aurantiacus OK-70-fl/DSM636. Exact OK-70-fl MCR, PCS, Mcl and Smt sequences are distinguished from J-10-fl BC1 and current inferred CT/SDH annotations. BC1 has a fused BCCP domain and forms a tetramer; no whole-ACC stoichiometry is inferred. Current native MCR/Smt component cross-references refer to different J-10-fl sequences; the selected OK-70-fl components are independently grounded. PCS has an unresolved three-or-four-subunit native estimate. Physiological MCR metal preference and the SDH quinone species remain unresolved; Mg/Fe stimulation and artificial DCIP/PMS assays are not promoted to exact physiological assignments. The genome-paper fumarase locus Caur_1443 is currently annotated as aspartase, so fumarase chemistry is retained without an invented catalyst. Exact propionyl-CoA carboxylase, methylmalonyl-CoA epimerase and mutase isoform assignments likewise remain open. Native archaeal carboxylase CPLX-7545 and reductase MONOMER-13611 are excluded from bacterial protein attribution. Thus the complete chemical cycle is represented with visible protein-identity gaps, not a claim that every catalytic component or assembly has been experimentally fixed in a single strain.

Pathway type

carbon fixation

Organism scope

Chloroflexus aurantiacus NCBITaxon:1108

Read the source YAML

Pathway network

66 nodes · 121 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Find a node

Reaction / activity
Chemical / cofactor
Protein / complex
Pathway
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

Show at full size (scroll to explore)

Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Chloroflexus aurantiacus `NCBITaxon:1108`	taxon
1,4-benzoquinones `CHEBI:132124`	chemical entity
water `CHEBI:15377`	small molecule
hydron `CHEBI:15378`	small molecule
(S)-malate(2-) `CHEBI:15589`	small molecule
3-hydroxypropionate `CHEBI:16510`	small molecule
carbon dioxide `CHEBI:16526`	small molecule
hydrogencarbonate `CHEBI:17544`	small molecule
hydroquinones `CHEBI:24646`	chemical entity
fumarate(2-) `CHEBI:29806`	small molecule
succinate(2-) `CHEBI:30031`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
diphosphate(3-) `CHEBI:33019`	small molecule
3-oxopropanoate `CHEBI:33190`	small molecule
glyoxylate `CHEBI:36655`	small molecule
hydrogenphosphate `CHEBI:43474`	small molecule
adenosine 5'-monophosphate(2-) `CHEBI:456215`	small molecule
ADP(3-) `CHEBI:456216`	small molecule
coenzyme A(4-) `CHEBI:57287`	small molecule
acetyl-CoA(4-) `CHEBI:57288`	small molecule
succinyl-CoA(5-) `CHEBI:57292`	small molecule
(3S)-3-carboxy-3-hydroxypropanoyl-CoA(5-) `CHEBI:57317`	small molecule
(R)-methylmalonyl-CoA(5-) `CHEBI:57326`	small molecule
(S)-methylmalonyl-CoA(5-) `CHEBI:57327`	small molecule
acryloyl-CoA(4-) `CHEBI:57367`	small molecule
malonyl-CoA(5-) `CHEBI:57384`	small molecule
propionyl-CoA(4-) `CHEBI:57392`	small molecule
NADPH(4-) `CHEBI:57783`	small molecule
NADP(3-) `CHEBI:58349`	small molecule
3-hydroxypropanoyl-CoA(4-) `CHEBI:58528`	small molecule
N(6)-biotinyl-L-lysyl-[protein] `RHEA-COMP:10505`	protein
N(6)-carboxybiotinyl-L-lysyl-[protein] `RHEA-COMP:10506`	protein
magnesium(2+) `CHEBI:18420`	cofactor
manganese(2+) `CHEBI:29035`	cofactor
zinc(2+) `CHEBI:29105`	cofactor
biotinate `CHEBI:57586`	cofactor
FAD(3-) `CHEBI:57692`	cofactor
potassium(1+) `CHEBI:29103`	cofactor
biotin carboxylase `UniProtKB:A9W9X0`	protein
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha `UniProtKB:A9WBQ8`	protein
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta `UniProtKB:A9WBQ9`	protein
succinate dehydrogenase `UniProtKB:A9WDT7`	protein
Malonyl-CoA reductase `UniProtKB:Q6QQP7`	protein
Malyl-CoA/beta-methylmalyl-CoA/citramalyl-CoA lyase `UniProtKB:S5N020`	protein
Succinyl-CoA--L-malate CoA-transferase alpha subunit `UniProtKB:Q1KLK1`	protein
Succinyl-CoA--L-malate CoA-transferase beta subunit `UniProtKB:Q1KLK0`	protein
acetate--CoA ligase `UniProtKB:Q8VRG6`	protein
malonyl CoA reductase `MetaCyc:CPLX-7550`	complex
L-malyl CoA lyase `MetaCyc:CPLX-7549`	complex
succinyl CoA:L-malate CoA transferase `MetaCyc:CPLX-7546`	complex
succinyl CoA:L-malate CoA transferase dimer `MetaCyc:CPLX-7548`	complex
CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	reaction	right to left
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	molecular activity	left to right
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	molecular activity	left to right
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	molecular activity	right to left
3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	molecular activity	right to left
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	molecular activity	left to right
3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	molecular activity	left to right
acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	molecular activity	right to left
propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	molecular activity	left to right
(S)-methylmalonyl-CoA => (R)-methylmalonyl-CoA `RHEA:20555`	molecular activity	right to left
(R)-methylmalonyl-CoA => succinyl-CoA `RHEA:22889`	molecular activity	left to right
succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	molecular activity	left to right
a quinone + succinate => fumarate + a quinol `RHEA:40524`	molecular activity	left to right
fumarate + H2O => (S)-malate `RHEA:12462`	molecular activity	right to left
(S)-malyl-CoA => glyoxylate + acetyl-CoA `RHEA:16630`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
carbon dioxide `CHEBI:16526`	consumes Stoichiometric coefficient 1.	CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	RHEA:10750 Source assertion: Rhea directed reaction RHEA:10750 places CHEBI:16526 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/10750 rh:substrates http://rdf.rhea-db.org/10748_R; http://rdf.rhea-db.org/10748_R rh:contains1 http://rdf.rhea-db.org/Participant_10748_compound_4391; http://rdf.rhea-db.org/Participant_10748_compound_4391 rh:compound http://rdf.rhea-db.org/Compound_4391; rh:accession`
water `CHEBI:15377`	consumes Stoichiometric coefficient 1.	CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	RHEA:10750 Source assertion: Rhea directed reaction RHEA:10750 places CHEBI:15377 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/10750 rh:substrates http://rdf.rhea-db.org/10748_R; http://rdf.rhea-db.org/10748_R rh:contains1 http://rdf.rhea-db.org/Participant_10748_compound_1283; http://rdf.rhea-db.org/Participant_10748_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; rh:accession`
CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	produces Stoichiometric coefficient 1.	hydrogencarbonate `CHEBI:17544`	RHEA:10750 Source assertion: Rhea directed reaction RHEA:10750 places CHEBI:17544 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/10750 rh:products http://rdf.rhea-db.org/10748_L; http://rdf.rhea-db.org/10748_L rh:contains1 http://rdf.rhea-db.org/Participant_10748_compound_1123; http://rdf.rhea-db.org/Participant_10748_compound_1123 rh:compound http://rdf.rhea-db.org/Compound_1123; rh:accession`
CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	produces Stoichiometric coefficient 1.	hydron `CHEBI:15378`	RHEA:10750 Source assertion: Rhea directed reaction RHEA:10750 places CHEBI:15378 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/10750 rh:products http://rdf.rhea-db.org/10748_L; http://rdf.rhea-db.org/10748_L rh:contains1 http://rdf.rhea-db.org/Participant_10748_compound_3249; http://rdf.rhea-db.org/Participant_10748_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
fumarate(2-) `CHEBI:29806`	consumes Stoichiometric coefficient 1.	fumarate + H2O => (S)-malate `RHEA:12462`	RHEA:12462 Source assertion: Rhea directed reaction RHEA:12462 places CHEBI:29806 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/12462 rh:substrates http://rdf.rhea-db.org/12460_R; http://rdf.rhea-db.org/12460_R rh:contains1 http://rdf.rhea-db.org/Participant_12460_compound_3031; http://rdf.rhea-db.org/Participant_12460_compound_3031 rh:compound http://rdf.rhea-db.org/Compound_3031; rh:accession`
water `CHEBI:15377`	consumes Stoichiometric coefficient 1.	fumarate + H2O => (S)-malate `RHEA:12462`	RHEA:12462 Source assertion: Rhea directed reaction RHEA:12462 places CHEBI:15377 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/12462 rh:substrates http://rdf.rhea-db.org/12460_R; http://rdf.rhea-db.org/12460_R rh:contains1 http://rdf.rhea-db.org/Participant_12460_compound_1283; http://rdf.rhea-db.org/Participant_12460_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; rh:accession`
fumarate + H2O => (S)-malate `RHEA:12462`	produces Stoichiometric coefficient 1.	(S)-malate(2-) `CHEBI:15589`	RHEA:12462 Source assertion: Rhea directed reaction RHEA:12462 places CHEBI:15589 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/12462 rh:products http://rdf.rhea-db.org/12460_L; http://rdf.rhea-db.org/12460_L rh:contains1 http://rdf.rhea-db.org/Participant_12460_compound_3953; http://rdf.rhea-db.org/Participant_12460_compound_3953 rh:compound http://rdf.rhea-db.org/Compound_3953; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] `RHEA-COMP:10505`	consumes Stoichiometric coefficient 1.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:13502 Source assertion: Rhea directed reaction RHEA:13502 places RHEA-COMP:10505 on its substrates side with coefficient 1. This is the complete generic protein-bearing carrier state, not its isolated lysine residue. Source location `http://rdf.rhea-db.org/13502 rh:substrates http://rdf.rhea-db.org/13501_L; http://rdf.rhea-db.org/13501_L rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_10505; http://rdf.rhea-db.org/Participant_13501_compound_10505 rh:compound http://rdf.rhea-db.org/Compound_10505; rh:accession`
hydrogencarbonate `CHEBI:17544`	consumes Stoichiometric coefficient 1.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:13502 Source assertion: Rhea directed reaction RHEA:13502 places CHEBI:17544 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:substrates http://rdf.rhea-db.org/13501_L; http://rdf.rhea-db.org/13501_L rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_1123; http://rdf.rhea-db.org/Participant_13501_compound_1123 rh:compound http://rdf.rhea-db.org/Compound_1123; rh:accession`
ATP(4-) `CHEBI:30616`	consumes Stoichiometric coefficient 1.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:13502 Source assertion: Rhea directed reaction RHEA:13502 places CHEBI:30616 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:substrates http://rdf.rhea-db.org/13501_L; http://rdf.rhea-db.org/13501_L rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_6372; http://rdf.rhea-db.org/Participant_13501_compound_6372 rh:compound http://rdf.rhea-db.org/Compound_6372; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	produces Stoichiometric coefficient 1.	N(6)-carboxybiotinyl-L-lysyl-[protein] `RHEA-COMP:10506`	RHEA:13502 Source assertion: Rhea directed reaction RHEA:13502 places RHEA-COMP:10506 on its products side with coefficient 1. This is the complete generic protein-bearing carrier state, not its isolated lysine residue. Source location `http://rdf.rhea-db.org/13502 rh:products http://rdf.rhea-db.org/13501_R; http://rdf.rhea-db.org/13501_R rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_10506; http://rdf.rhea-db.org/Participant_13501_compound_10506 rh:compound http://rdf.rhea-db.org/Compound_10506; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	produces Stoichiometric coefficient 1.	ADP(3-) `CHEBI:456216`	RHEA:13502 Source assertion: Rhea directed reaction RHEA:13502 places CHEBI:456216 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:products http://rdf.rhea-db.org/13501_R; http://rdf.rhea-db.org/13501_R rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_2710; http://rdf.rhea-db.org/Participant_13501_compound_2710 rh:compound http://rdf.rhea-db.org/Compound_2710; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	produces Stoichiometric coefficient 1.	hydrogenphosphate `CHEBI:43474`	RHEA:13502 Source assertion: Rhea directed reaction RHEA:13502 places CHEBI:43474 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:products http://rdf.rhea-db.org/13501_R; http://rdf.rhea-db.org/13501_R rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_5518; http://rdf.rhea-db.org/Participant_13501_compound_5518 rh:compound http://rdf.rhea-db.org/Compound_5518; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	produces Stoichiometric coefficient 1.	hydron `CHEBI:15378`	RHEA:13502 Source assertion: Rhea directed reaction RHEA:13502 places CHEBI:15378 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:products http://rdf.rhea-db.org/13501_R; http://rdf.rhea-db.org/13501_R rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_3249; http://rdf.rhea-db.org/Participant_13501_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
(3S)-3-carboxy-3-hydroxypropanoyl-CoA(5-) `CHEBI:57317`	consumes Stoichiometric coefficient 1.	(S)-malyl-CoA => glyoxylate + acetyl-CoA `RHEA:16630`	RHEA:16630 Source assertion: Rhea directed reaction RHEA:16630 places CHEBI:57317 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/16630 rh:substrates http://rdf.rhea-db.org/16629_L; http://rdf.rhea-db.org/16629_L rh:contains1 http://rdf.rhea-db.org/Participant_16629_compound_3610; http://rdf.rhea-db.org/Participant_16629_compound_3610 rh:compound http://rdf.rhea-db.org/Compound_3610; rh:accession`
(S)-malyl-CoA => glyoxylate + acetyl-CoA `RHEA:16630`	produces Stoichiometric coefficient 1.	glyoxylate `CHEBI:36655`	RHEA:16630 Source assertion: Rhea directed reaction RHEA:16630 places CHEBI:36655 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/16630 rh:products http://rdf.rhea-db.org/16629_R; http://rdf.rhea-db.org/16629_R rh:contains1 http://rdf.rhea-db.org/Participant_16629_compound_3124; http://rdf.rhea-db.org/Participant_16629_compound_3124 rh:compound http://rdf.rhea-db.org/Compound_3124; rh:accession`
(S)-malyl-CoA => glyoxylate + acetyl-CoA `RHEA:16630`	produces Stoichiometric coefficient 1.	acetyl-CoA(4-) `CHEBI:57288`	RHEA:16630 Source assertion: Rhea directed reaction RHEA:16630 places CHEBI:57288 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/16630 rh:products http://rdf.rhea-db.org/16629_R; http://rdf.rhea-db.org/16629_R rh:contains1 http://rdf.rhea-db.org/Participant_16629_compound_4271; http://rdf.rhea-db.org/Participant_16629_compound_4271 rh:compound http://rdf.rhea-db.org/Compound_4271; rh:accession`
(S)-methylmalonyl-CoA(5-) `CHEBI:57327`	consumes Stoichiometric coefficient 1.	(S)-methylmalonyl-CoA => (R)-methylmalonyl-CoA `RHEA:20555`	RHEA:20555 Source assertion: Rhea directed reaction RHEA:20555 places CHEBI:57327 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/20555 rh:substrates http://rdf.rhea-db.org/20553_R; http://rdf.rhea-db.org/20553_R rh:contains1 http://rdf.rhea-db.org/Participant_20553_compound_2780; http://rdf.rhea-db.org/Participant_20553_compound_2780 rh:compound http://rdf.rhea-db.org/Compound_2780; rh:accession`
(S)-methylmalonyl-CoA => (R)-methylmalonyl-CoA `RHEA:20555`	produces Stoichiometric coefficient 1.	(R)-methylmalonyl-CoA(5-) `CHEBI:57326`	RHEA:20555 Source assertion: Rhea directed reaction RHEA:20555 places CHEBI:57326 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/20555 rh:products http://rdf.rhea-db.org/20553_L; http://rdf.rhea-db.org/20553_L rh:contains1 http://rdf.rhea-db.org/Participant_20553_compound_1391; http://rdf.rhea-db.org/Participant_20553_compound_1391 rh:compound http://rdf.rhea-db.org/Compound_1391; rh:accession`
(R)-methylmalonyl-CoA(5-) `CHEBI:57326`	consumes Stoichiometric coefficient 1.	(R)-methylmalonyl-CoA => succinyl-CoA `RHEA:22889`	RHEA:22889 Source assertion: Rhea directed reaction RHEA:22889 places CHEBI:57326 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/22889 rh:substrates http://rdf.rhea-db.org/22888_L; http://rdf.rhea-db.org/22888_L rh:contains1 http://rdf.rhea-db.org/Participant_22888_compound_1391; http://rdf.rhea-db.org/Participant_22888_compound_1391 rh:compound http://rdf.rhea-db.org/Compound_1391; rh:accession`
(R)-methylmalonyl-CoA => succinyl-CoA `RHEA:22889`	produces Stoichiometric coefficient 1.	succinyl-CoA(5-) `CHEBI:57292`	RHEA:22889 Source assertion: Rhea directed reaction RHEA:22889 places CHEBI:57292 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/22889 rh:products http://rdf.rhea-db.org/22888_R; http://rdf.rhea-db.org/22888_R rh:contains1 http://rdf.rhea-db.org/Participant_22888_compound_4684; http://rdf.rhea-db.org/Participant_22888_compound_4684 rh:compound http://rdf.rhea-db.org/Compound_4684; rh:accession`
propionyl-CoA(4-) `CHEBI:57392`	consumes Stoichiometric coefficient 1.	propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	RHEA:23721 Source assertion: Rhea directed reaction RHEA:23721 places CHEBI:57392 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/23721 rh:substrates http://rdf.rhea-db.org/23720_L; http://rdf.rhea-db.org/23720_L rh:contains1 http://rdf.rhea-db.org/Participant_23720_compound_3421; http://rdf.rhea-db.org/Participant_23720_compound_3421 rh:compound http://rdf.rhea-db.org/Compound_3421; rh:accession`
hydrogencarbonate `CHEBI:17544`	consumes Stoichiometric coefficient 1.	propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	RHEA:23721 Source assertion: Rhea directed reaction RHEA:23721 places CHEBI:17544 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/23721 rh:substrates http://rdf.rhea-db.org/23720_L; http://rdf.rhea-db.org/23720_L rh:contains1 http://rdf.rhea-db.org/Participant_23720_compound_1123; http://rdf.rhea-db.org/Participant_23720_compound_1123 rh:compound http://rdf.rhea-db.org/Compound_1123; rh:accession`
ATP(4-) `CHEBI:30616`	consumes Stoichiometric coefficient 1.	propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	RHEA:23721 Source assertion: Rhea directed reaction RHEA:23721 places CHEBI:30616 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/23721 rh:substrates http://rdf.rhea-db.org/23720_L; http://rdf.rhea-db.org/23720_L rh:contains1 http://rdf.rhea-db.org/Participant_23720_compound_6372; http://rdf.rhea-db.org/Participant_23720_compound_6372 rh:compound http://rdf.rhea-db.org/Compound_6372; rh:accession`
propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	produces Stoichiometric coefficient 1.	(S)-methylmalonyl-CoA(5-) `CHEBI:57327`	RHEA:23721 Source assertion: Rhea directed reaction RHEA:23721 places CHEBI:57327 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/23721 rh:products http://rdf.rhea-db.org/23720_R; http://rdf.rhea-db.org/23720_R rh:contains1 http://rdf.rhea-db.org/Participant_23720_compound_2780; http://rdf.rhea-db.org/Participant_23720_compound_2780 rh:compound http://rdf.rhea-db.org/Compound_2780; rh:accession`
propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	produces Stoichiometric coefficient 1.	ADP(3-) `CHEBI:456216`	RHEA:23721 Source assertion: Rhea directed reaction RHEA:23721 places CHEBI:456216 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/23721 rh:products http://rdf.rhea-db.org/23720_R; http://rdf.rhea-db.org/23720_R rh:contains1 http://rdf.rhea-db.org/Participant_23720_compound_2710; http://rdf.rhea-db.org/Participant_23720_compound_2710 rh:compound http://rdf.rhea-db.org/Compound_2710; rh:accession`
propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	produces Stoichiometric coefficient 1.	hydrogenphosphate `CHEBI:43474`	RHEA:23721 Source assertion: Rhea directed reaction RHEA:23721 places CHEBI:43474 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/23721 rh:products http://rdf.rhea-db.org/23720_R; http://rdf.rhea-db.org/23720_R rh:contains1 http://rdf.rhea-db.org/Participant_23720_compound_5518; http://rdf.rhea-db.org/Participant_23720_compound_5518 rh:compound http://rdf.rhea-db.org/Compound_5518; rh:accession`
propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	produces Stoichiometric coefficient 1.	hydron `CHEBI:15378`	RHEA:23721 Source assertion: Rhea directed reaction RHEA:23721 places CHEBI:15378 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/23721 rh:products http://rdf.rhea-db.org/23720_R; http://rdf.rhea-db.org/23720_R rh:contains1 http://rdf.rhea-db.org/Participant_23720_compound_3249; http://rdf.rhea-db.org/Participant_23720_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
3-oxopropanoate `CHEBI:33190`	consumes Stoichiometric coefficient 1.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	RHEA:26440 Source assertion: Rhea directed reaction RHEA:26440 places CHEBI:33190 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:substrates http://rdf.rhea-db.org/26438_R; http://rdf.rhea-db.org/26438_R rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_5274; http://rdf.rhea-db.org/Participant_26438_compound_5274 rh:compound http://rdf.rhea-db.org/Compound_5274; rh:accession`
NADPH(4-) `CHEBI:57783`	consumes Stoichiometric coefficient 1.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	RHEA:26440 Source assertion: Rhea directed reaction RHEA:26440 places CHEBI:57783 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:substrates http://rdf.rhea-db.org/26438_R; http://rdf.rhea-db.org/26438_R rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_3438; http://rdf.rhea-db.org/Participant_26438_compound_3438 rh:compound http://rdf.rhea-db.org/Compound_3438; rh:accession`
hydron `CHEBI:15378`	consumes Stoichiometric coefficient 1.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	RHEA:26440 Source assertion: Rhea directed reaction RHEA:26440 places CHEBI:15378 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:substrates http://rdf.rhea-db.org/26438_R; http://rdf.rhea-db.org/26438_R rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_3249; http://rdf.rhea-db.org/Participant_26438_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	produces Stoichiometric coefficient 1.	3-hydroxypropionate `CHEBI:16510`	RHEA:26440 Source assertion: Rhea directed reaction RHEA:26440 places CHEBI:16510 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:products http://rdf.rhea-db.org/26438_L; http://rdf.rhea-db.org/26438_L rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_3378; http://rdf.rhea-db.org/Participant_26438_compound_3378 rh:compound http://rdf.rhea-db.org/Compound_3378; rh:accession`
3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	produces Stoichiometric coefficient 1.	NADP(3-) `CHEBI:58349`	RHEA:26440 Source assertion: Rhea directed reaction RHEA:26440 places CHEBI:58349 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:products http://rdf.rhea-db.org/26438_L; http://rdf.rhea-db.org/26438_L rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_5188; http://rdf.rhea-db.org/Participant_26438_compound_5188 rh:compound http://rdf.rhea-db.org/Compound_5188; rh:accession`
malonyl-CoA(5-) `CHEBI:57384`	consumes Stoichiometric coefficient 1.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	RHEA:26448 Source assertion: Rhea directed reaction RHEA:26448 places CHEBI:57384 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:substrates http://rdf.rhea-db.org/26446_R; http://rdf.rhea-db.org/26446_R rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_5125; http://rdf.rhea-db.org/Participant_26446_compound_5125 rh:compound http://rdf.rhea-db.org/Compound_5125; rh:accession`
NADPH(4-) `CHEBI:57783`	consumes Stoichiometric coefficient 1.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	RHEA:26448 Source assertion: Rhea directed reaction RHEA:26448 places CHEBI:57783 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:substrates http://rdf.rhea-db.org/26446_R; http://rdf.rhea-db.org/26446_R rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_3438; http://rdf.rhea-db.org/Participant_26446_compound_3438 rh:compound http://rdf.rhea-db.org/Compound_3438; rh:accession`
hydron `CHEBI:15378`	consumes Stoichiometric coefficient 1.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	RHEA:26448 Source assertion: Rhea directed reaction RHEA:26448 places CHEBI:15378 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:substrates http://rdf.rhea-db.org/26446_R; http://rdf.rhea-db.org/26446_R rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_3249; http://rdf.rhea-db.org/Participant_26446_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	produces Stoichiometric coefficient 1.	3-oxopropanoate `CHEBI:33190`	RHEA:26448 Source assertion: Rhea directed reaction RHEA:26448 places CHEBI:33190 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:products http://rdf.rhea-db.org/26446_L; http://rdf.rhea-db.org/26446_L rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_5274; http://rdf.rhea-db.org/Participant_26446_compound_5274 rh:compound http://rdf.rhea-db.org/Compound_5274; rh:accession`
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	produces Stoichiometric coefficient 1.	NADP(3-) `CHEBI:58349`	RHEA:26448 Source assertion: Rhea directed reaction RHEA:26448 places CHEBI:58349 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:products http://rdf.rhea-db.org/26446_L; http://rdf.rhea-db.org/26446_L rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_5188; http://rdf.rhea-db.org/Participant_26446_compound_5188 rh:compound http://rdf.rhea-db.org/Compound_5188; rh:accession`
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	produces Stoichiometric coefficient 1.	coenzyme A(4-) `CHEBI:57287`	RHEA:26448 Source assertion: Rhea directed reaction RHEA:26448 places CHEBI:57287 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:products http://rdf.rhea-db.org/26446_L; http://rdf.rhea-db.org/26446_L rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_1264; http://rdf.rhea-db.org/Participant_26446_compound_1264 rh:compound http://rdf.rhea-db.org/Compound_1264; rh:accession`
acryloyl-CoA(4-) `CHEBI:57367`	consumes Stoichiometric coefficient 1.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	RHEA:26456 Source assertion: Rhea directed reaction RHEA:26456 places CHEBI:57367 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:substrates http://rdf.rhea-db.org/26454_R; http://rdf.rhea-db.org/26454_R rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_2006; http://rdf.rhea-db.org/Participant_26454_compound_2006 rh:compound http://rdf.rhea-db.org/Compound_2006; rh:accession`
NADPH(4-) `CHEBI:57783`	consumes Stoichiometric coefficient 1.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	RHEA:26456 Source assertion: Rhea directed reaction RHEA:26456 places CHEBI:57783 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:substrates http://rdf.rhea-db.org/26454_R; http://rdf.rhea-db.org/26454_R rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_3438; http://rdf.rhea-db.org/Participant_26454_compound_3438 rh:compound http://rdf.rhea-db.org/Compound_3438; rh:accession`
hydron `CHEBI:15378`	consumes Stoichiometric coefficient 1.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	RHEA:26456 Source assertion: Rhea directed reaction RHEA:26456 places CHEBI:15378 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:substrates http://rdf.rhea-db.org/26454_R; http://rdf.rhea-db.org/26454_R rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_3249; http://rdf.rhea-db.org/Participant_26454_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	produces Stoichiometric coefficient 1.	propionyl-CoA(4-) `CHEBI:57392`	RHEA:26456 Source assertion: Rhea directed reaction RHEA:26456 places CHEBI:57392 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:products http://rdf.rhea-db.org/26454_L; http://rdf.rhea-db.org/26454_L rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_3421; http://rdf.rhea-db.org/Participant_26454_compound_3421 rh:compound http://rdf.rhea-db.org/Compound_3421; rh:accession`
acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	produces Stoichiometric coefficient 1.	NADP(3-) `CHEBI:58349`	RHEA:26456 Source assertion: Rhea directed reaction RHEA:26456 places CHEBI:58349 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:products http://rdf.rhea-db.org/26454_L; http://rdf.rhea-db.org/26454_L rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_5188; http://rdf.rhea-db.org/Participant_26454_compound_5188 rh:compound http://rdf.rhea-db.org/Compound_5188; rh:accession`
3-hydroxypropanoyl-CoA(4-) `CHEBI:58528`	consumes Stoichiometric coefficient 1.	3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	RHEA:26519 Source assertion: Rhea directed reaction RHEA:26519 places CHEBI:58528 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26519 rh:substrates http://rdf.rhea-db.org/26518_L; http://rdf.rhea-db.org/26518_L rh:contains1 http://rdf.rhea-db.org/Participant_26518_compound_6030; http://rdf.rhea-db.org/Participant_26518_compound_6030 rh:compound http://rdf.rhea-db.org/Compound_6030; rh:accession`
3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	produces Stoichiometric coefficient 1.	acryloyl-CoA(4-) `CHEBI:57367`	RHEA:26519 Source assertion: Rhea directed reaction RHEA:26519 places CHEBI:57367 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26519 rh:products http://rdf.rhea-db.org/26518_R; http://rdf.rhea-db.org/26518_R rh:contains1 http://rdf.rhea-db.org/Participant_26518_compound_2006; http://rdf.rhea-db.org/Participant_26518_compound_2006 rh:compound http://rdf.rhea-db.org/Compound_2006; rh:accession`
3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	produces Stoichiometric coefficient 1.	water `CHEBI:15377`	RHEA:26519 Source assertion: Rhea directed reaction RHEA:26519 places CHEBI:15377 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26519 rh:products http://rdf.rhea-db.org/26518_R; http://rdf.rhea-db.org/26518_R rh:contains1 http://rdf.rhea-db.org/Participant_26518_compound_1283; http://rdf.rhea-db.org/Participant_26518_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; rh:accession`
3-hydroxypropionate `CHEBI:16510`	consumes Stoichiometric coefficient 1.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	RHEA:26535 Source assertion: Rhea directed reaction RHEA:26535 places CHEBI:16510 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:substrates http://rdf.rhea-db.org/26534_L; http://rdf.rhea-db.org/26534_L rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_3378; http://rdf.rhea-db.org/Participant_26534_compound_3378 rh:compound http://rdf.rhea-db.org/Compound_3378; rh:accession`
ATP(4-) `CHEBI:30616`	consumes Stoichiometric coefficient 1.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	RHEA:26535 Source assertion: Rhea directed reaction RHEA:26535 places CHEBI:30616 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:substrates http://rdf.rhea-db.org/26534_L; http://rdf.rhea-db.org/26534_L rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_6372; http://rdf.rhea-db.org/Participant_26534_compound_6372 rh:compound http://rdf.rhea-db.org/Compound_6372; rh:accession`
coenzyme A(4-) `CHEBI:57287`	consumes Stoichiometric coefficient 1.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	RHEA:26535 Source assertion: Rhea directed reaction RHEA:26535 places CHEBI:57287 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:substrates http://rdf.rhea-db.org/26534_L; http://rdf.rhea-db.org/26534_L rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_1264; http://rdf.rhea-db.org/Participant_26534_compound_1264 rh:compound http://rdf.rhea-db.org/Compound_1264; rh:accession`
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	produces Stoichiometric coefficient 1.	3-hydroxypropanoyl-CoA(4-) `CHEBI:58528`	RHEA:26535 Source assertion: Rhea directed reaction RHEA:26535 places CHEBI:58528 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:products http://rdf.rhea-db.org/26534_R; http://rdf.rhea-db.org/26534_R rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_6030; http://rdf.rhea-db.org/Participant_26534_compound_6030 rh:compound http://rdf.rhea-db.org/Compound_6030; rh:accession`
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	produces Stoichiometric coefficient 1.	adenosine 5'-monophosphate(2-) `CHEBI:456215`	RHEA:26535 Source assertion: Rhea directed reaction RHEA:26535 places CHEBI:456215 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:products http://rdf.rhea-db.org/26534_R; http://rdf.rhea-db.org/26534_R rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_2590; http://rdf.rhea-db.org/Participant_26534_compound_2590 rh:compound http://rdf.rhea-db.org/Compound_2590; rh:accession`
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	produces Stoichiometric coefficient 1.	diphosphate(3-) `CHEBI:33019`	RHEA:26535 Source assertion: Rhea directed reaction RHEA:26535 places CHEBI:33019 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:products http://rdf.rhea-db.org/26534_R; http://rdf.rhea-db.org/26534_R rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_2106; http://rdf.rhea-db.org/Participant_26534_compound_2106 rh:compound http://rdf.rhea-db.org/Compound_2106; rh:accession`
succinyl-CoA(5-) `CHEBI:57292`	consumes Stoichiometric coefficient 1.	succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	RHEA:38256 Source assertion: Rhea directed reaction RHEA:38256 places CHEBI:57292 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38256 rh:substrates http://rdf.rhea-db.org/38255_L; http://rdf.rhea-db.org/38255_L rh:contains1 http://rdf.rhea-db.org/Participant_38255_compound_4684; http://rdf.rhea-db.org/Participant_38255_compound_4684 rh:compound http://rdf.rhea-db.org/Compound_4684; rh:accession`
(S)-malate(2-) `CHEBI:15589`	consumes Stoichiometric coefficient 1.	succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	RHEA:38256 Source assertion: Rhea directed reaction RHEA:38256 places CHEBI:15589 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38256 rh:substrates http://rdf.rhea-db.org/38255_L; http://rdf.rhea-db.org/38255_L rh:contains1 http://rdf.rhea-db.org/Participant_38255_compound_3953; http://rdf.rhea-db.org/Participant_38255_compound_3953 rh:compound http://rdf.rhea-db.org/Compound_3953; rh:accession`
succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	produces Stoichiometric coefficient 1.	(3S)-3-carboxy-3-hydroxypropanoyl-CoA(5-) `CHEBI:57317`	RHEA:38256 Source assertion: Rhea directed reaction RHEA:38256 places CHEBI:57317 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38256 rh:products http://rdf.rhea-db.org/38255_R; http://rdf.rhea-db.org/38255_R rh:contains1 http://rdf.rhea-db.org/Participant_38255_compound_3610; http://rdf.rhea-db.org/Participant_38255_compound_3610 rh:compound http://rdf.rhea-db.org/Compound_3610; rh:accession`
succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	produces Stoichiometric coefficient 1.	succinate(2-) `CHEBI:30031`	RHEA:38256 Source assertion: Rhea directed reaction RHEA:38256 places CHEBI:30031 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38256 rh:products http://rdf.rhea-db.org/38255_R; http://rdf.rhea-db.org/38255_R rh:contains1 http://rdf.rhea-db.org/Participant_38255_compound_2501; http://rdf.rhea-db.org/Participant_38255_compound_2501 rh:compound http://rdf.rhea-db.org/Compound_2501; rh:accession`
1,4-benzoquinones `CHEBI:132124`	consumes Stoichiometric coefficient 1.	a quinone + succinate => fumarate + a quinol `RHEA:40524`	RHEA:40524 Source assertion: Rhea directed reaction RHEA:40524 places CHEBI:132124 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/40524 rh:substrates http://rdf.rhea-db.org/40523_L; http://rdf.rhea-db.org/40523_L rh:contains1 http://rdf.rhea-db.org/Participant_40523_compound_13027; http://rdf.rhea-db.org/Participant_40523_compound_13027 rh:compound http://rdf.rhea-db.org/Compound_13027; rh:accession`
succinate(2-) `CHEBI:30031`	consumes Stoichiometric coefficient 1.	a quinone + succinate => fumarate + a quinol `RHEA:40524`	RHEA:40524 Source assertion: Rhea directed reaction RHEA:40524 places CHEBI:30031 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/40524 rh:substrates http://rdf.rhea-db.org/40523_L; http://rdf.rhea-db.org/40523_L rh:contains1 http://rdf.rhea-db.org/Participant_40523_compound_2501; http://rdf.rhea-db.org/Participant_40523_compound_2501 rh:compound http://rdf.rhea-db.org/Compound_2501; rh:accession`
a quinone + succinate => fumarate + a quinol `RHEA:40524`	produces Stoichiometric coefficient 1.	fumarate(2-) `CHEBI:29806`	RHEA:40524 Source assertion: Rhea directed reaction RHEA:40524 places CHEBI:29806 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/40524 rh:products http://rdf.rhea-db.org/40523_R; http://rdf.rhea-db.org/40523_R rh:contains1 http://rdf.rhea-db.org/Participant_40523_compound_3031; http://rdf.rhea-db.org/Participant_40523_compound_3031 rh:compound http://rdf.rhea-db.org/Compound_3031; rh:accession`
a quinone + succinate => fumarate + a quinol `RHEA:40524`	produces Stoichiometric coefficient 1.	hydroquinones `CHEBI:24646`	RHEA:40524 Source assertion: Rhea directed reaction RHEA:40524 places CHEBI:24646 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/40524 rh:products http://rdf.rhea-db.org/40523_R; http://rdf.rhea-db.org/40523_R rh:contains1 http://rdf.rhea-db.org/Participant_40523_compound_13026; http://rdf.rhea-db.org/Participant_40523_compound_13026 rh:compound http://rdf.rhea-db.org/Compound_13026; rh:accession`
N(6)-carboxybiotinyl-L-lysyl-[protein] `RHEA-COMP:10506`	consumes Stoichiometric coefficient 1.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	RHEA:54729 Source assertion: Rhea directed reaction RHEA:54729 places RHEA-COMP:10506 on its substrates side with coefficient 1. This is the complete generic protein-bearing carrier state, not its isolated lysine residue. Source location `http://rdf.rhea-db.org/54729 rh:substrates http://rdf.rhea-db.org/54728_L; http://rdf.rhea-db.org/54728_L rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_10506; http://rdf.rhea-db.org/Participant_54728_compound_10506 rh:compound http://rdf.rhea-db.org/Compound_10506; rh:accession`
acetyl-CoA(4-) `CHEBI:57288`	consumes Stoichiometric coefficient 1.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	RHEA:54729 Source assertion: Rhea directed reaction RHEA:54729 places CHEBI:57288 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/54729 rh:substrates http://rdf.rhea-db.org/54728_L; http://rdf.rhea-db.org/54728_L rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_4271; http://rdf.rhea-db.org/Participant_54728_compound_4271 rh:compound http://rdf.rhea-db.org/Compound_4271; rh:accession`
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	produces Stoichiometric coefficient 1.	N(6)-biotinyl-L-lysyl-[protein] `RHEA-COMP:10505`	RHEA:54729 Source assertion: Rhea directed reaction RHEA:54729 places RHEA-COMP:10505 on its products side with coefficient 1. This is the complete generic protein-bearing carrier state, not its isolated lysine residue. Source location `http://rdf.rhea-db.org/54729 rh:products http://rdf.rhea-db.org/54728_R; http://rdf.rhea-db.org/54728_R rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_10505; http://rdf.rhea-db.org/Participant_54728_compound_10505 rh:compound http://rdf.rhea-db.org/Compound_10505; rh:accession`
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	produces Stoichiometric coefficient 1.	malonyl-CoA(5-) `CHEBI:57384`	RHEA:54729 Source assertion: Rhea directed reaction RHEA:54729 places CHEBI:57384 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/54729 rh:products http://rdf.rhea-db.org/54728_R; http://rdf.rhea-db.org/54728_R rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_5125; http://rdf.rhea-db.org/Participant_54728_compound_5125 rh:compound http://rdf.rhea-db.org/Compound_5125; rh:accession`
`GO:0043427`	has_part Native inorganic-carbon equilibration is retained without assigning an unverified carbonic anhydrase; its turnover can supply bicarbonate to either carboxylation step.	CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists RXN0-5224 as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='RXN0-5224']` MetaCyc:RXN0-5224 Source assertion: The native reaction links Rhea 10751; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:10750 Source assertion: Rhea master 10748 explicitly links directed member 10750; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/10748 rh:directionalReaction http://rdf.rhea-db.org/10750; http://rdf.rhea-db.org/10750 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists BIOTIN-CARBOXYL-RXN as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='BIOTIN-CARBOXYL-RXN']` MetaCyc:BIOTIN-CARBOXYL-RXN Source assertion: The native reaction links Rhea 13502; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:13502 Source assertion: Rhea master 13501 explicitly links directed member 13502; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/13501 rh:directionalReaction http://rdf.rhea-db.org/13502; http://rdf.rhea-db.org/13502 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists RXN0-5055 as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='RXN0-5055']` MetaCyc:RXN0-5055 Source assertion: The native carboxyl-transfer reaction transfers the carboxyl group from carboxybiotin to acetyl-CoA; the current equation retains whole protein-bound biotin states. Source location `Reaction[@detail='full']/left; right` RHEA:54729 Source assertion: Rhea master 54728 explicitly links directed member 54729; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/54728 rh:directionalReaction http://rdf.rhea-db.org/54729; http://rdf.rhea-db.org/54729 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists RXN-8963 as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='RXN-8963']` MetaCyc:RXN-8963 Source assertion: The native reaction links Rhea 26449; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26448 Source assertion: Rhea master 26446 explicitly links directed member 26448; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/26446 rh:directionalReaction http://rdf.rhea-db.org/26448; http://rdf.rhea-db.org/26448 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists RXN-8974 as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='RXN-8974']` MetaCyc:RXN-8974 Source assertion: The native reaction links Rhea 26440; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26440 Source assertion: Rhea master 26438 explicitly links directed member 26440; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/26438 rh:directionalReaction http://rdf.rhea-db.org/26440; http://rdf.rhea-db.org/26440 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists RXN-9086 as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='RXN-9086']` MetaCyc:RXN-9086 Source assertion: The native reaction links Rhea 26535; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26535 Source assertion: Rhea master 26534 explicitly links directed member 26535; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/26534 rh:directionalReaction http://rdf.rhea-db.org/26535; http://rdf.rhea-db.org/26535 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded.	3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists RXN-6383 as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='RXN-6383']` MetaCyc:RXN-6383 Source assertion: The native reaction links Rhea 26518; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26519 Source assertion: Rhea master 26518 explicitly links directed member 26519; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/26518 rh:directionalReaction http://rdf.rhea-db.org/26519; http://rdf.rhea-db.org/26519 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists RXN-9087 as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='RXN-9087']` MetaCyc:RXN-9087 Source assertion: The native reaction links Rhea 26456; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26456 Source assertion: Rhea master 26454 explicitly links directed member 26456; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/26454 rh:directionalReaction http://rdf.rhea-db.org/26456; http://rdf.rhea-db.org/26456 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded. Exact Chloroflexus catalytic protein attribution remains unresolved; no archaeal catalyst is substituted.	propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists PROPIONYL-COA-CARBOXY-RXN as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='PROPIONYL-COA-CARBOXY-RXN']` MetaCyc:PROPIONYL-COA-CARBOXY-RXN Source assertion: The native reaction links Rhea 23723; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:23721 Source assertion: Rhea master 23720 explicitly links directed member 23721; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/23720 rh:directionalReaction http://rdf.rhea-db.org/23721; http://rdf.rhea-db.org/23721 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded. Exact Chloroflexus catalytic protein attribution remains unresolved; no archaeal catalyst is substituted.	(S)-methylmalonyl-CoA => (R)-methylmalonyl-CoA `RHEA:20555`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists METHYLMALONYL-COA-EPIM-RXN as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='METHYLMALONYL-COA-EPIM-RXN']` MetaCyc:METHYLMALONYL-COA-EPIM-RXN Source assertion: The native reaction links Rhea 20556; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:20555 Source assertion: Rhea master 20553 explicitly links directed member 20555; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/20553 rh:directionalReaction http://rdf.rhea-db.org/20555; http://rdf.rhea-db.org/20555 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded. Exact Chloroflexus catalytic protein attribution remains unresolved; no archaeal catalyst is substituted.	(R)-methylmalonyl-CoA => succinyl-CoA `RHEA:22889`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists METHYLMALONYL-COA-MUT-RXN as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='METHYLMALONYL-COA-MUT-RXN']` MetaCyc:METHYLMALONYL-COA-MUT-RXN Source assertion: The native reaction links Rhea 22891; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:22889 Source assertion: Rhea master 22888 explicitly links directed member 22889; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/22888 rh:directionalReaction http://rdf.rhea-db.org/22889; http://rdf.rhea-db.org/22889 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded.	succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists RXN-8964 as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='RXN-8964']` MetaCyc:RXN-8964 Source assertion: The native reaction links Rhea 38258; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:38256 Source assertion: Rhea master 38255 explicitly links directed member 38256; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/38255 rh:directionalReaction http://rdf.rhea-db.org/38256; http://rdf.rhea-db.org/38256 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded. The physiological quinone remains a class; the original DCIP/PMS assay does not identify a specific natural quinone.	a quinone + succinate => fumarate + a quinol `RHEA:40524`	PMID:11418572 Source assertion: The first-cycle diagram and extract assays include succinate dehydrogenase and fumarate hydratase to regenerate malate; these are missing from the captured PWY-5743 reaction list. Source location `Figure 1 activities 11–12; Materials and Methods, succinate dehydrogenase and fumarate hydratase assays; Results Table 1` RHEA:40524 Source assertion: Rhea master 40523 explicitly links directed member 40524; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/40523 rh:directionalReaction http://rdf.rhea-db.org/40524; http://rdf.rhea-db.org/40524 rh:substrates; rh:products` PMID:16547052 Source assertion: The authors state that one reduced quinone is produced during succinate oxidation to fumarate in a first-cycle turnover; this is pathway stoichiometry, not a direct identification of the physiological quinone species. Source location `Introduction, paragraph beginning One intriguing problem; Figure 1 activities 8–9`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded. Exact Chloroflexus catalytic protein attribution remains unresolved; no archaeal catalyst is substituted.	fumarate + H2O => (S)-malate `RHEA:12462`	PMID:11418572 Source assertion: The first-cycle diagram and extract assays include succinate dehydrogenase and fumarate hydratase to regenerate malate; these are missing from the captured PWY-5743 reaction list. Source location `Figure 1 activities 11–12; Materials and Methods, succinate dehydrogenase and fumarate hydratase assays; Results Table 1` RHEA:12462 Source assertion: Rhea master 12460 explicitly links directed member 12462; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/12460 rh:directionalReaction http://rdf.rhea-db.org/12462; http://rdf.rhea-db.org/12462 rh:substrates; rh:products`
`GO:0043427`	has_part Complete first-cycle chemistry; current Rhea equation and source-specific native mapping are independently grounded.	(S)-malyl-CoA => glyoxylate + acetyl-CoA `RHEA:16630`	MetaCyc:PWY-5743 Source assertion: PWY-5743 explicitly lists MALYL-COA-LYASE-RXN as a reaction member. Source location `Pathway[@frameid='PWY-5743']/reaction-list/Reaction[@frameid='MALYL-COA-LYASE-RXN']` MetaCyc:MALYL-COA-LYASE-RXN Source assertion: The native reaction links Rhea 16632; this supplies the reaction-family bridge, not an independent proof of physiological direction. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:16630 Source assertion: Rhea master 16629 explicitly links directed member 16630; its current substrate and product sides define the selected equation. Source location `http://rdf.rhea-db.org/16629 rh:directionalReaction http://rdf.rhea-db.org/16630; http://rdf.rhea-db.org/16630 rh:substrates; rh:products`
biotin carboxylase `UniProtKB:A9W9X0`	enables J-10-fl BC1 contains its own BCCP domain and forms a tetramer. This functional role does not assert a monomer or a fixed stoichiometry for the entire ACC assembly.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	PMID:38572988 Source assertion: BC1 from J-10-fl (Caur_1378) is a 596-residue biotin carboxylase fused to BCCP. Biotinylation and carboxylation assays establish its BC/carrier functions; solution analysis supports a tetramer. Source location `Results sections s2-1–s2-4; Materials and Methods s4-1, s4-3–s4-5, s4-8` UniProtKB:A9W9X0 Source assertion: The current Caur_1378 protein is biotin carboxylase, 596 residues, assigned to Chloroflexus aurantiacus J-10-fl. Source location `/proteinDescription/recommendedName/fullName; /genes; /organism; /sequence/length`
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha `UniProtKB:A9WBQ8`	enables Current J-10-fl HAMAP assignment for the joint alpha/beta carboxyltransferase function; neither subunit is asserted independently sufficient. The 2024 paper reverses locus/chain names, so it is not direct exact-accession assay evidence here.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	UniProtKB:A9WBQ8 Source assertion: The current catalytic annotation assigns the protein to Rhea 54728, transferring carboxyl from protein-bound carboxybiotin to acetyl-CoA. This is a HAMAP/inferred annotation, not a direct assay of this exact accession. Source annotation evidence: ECO:0000256. Source location `/comments/0/texts; /comments/1/reaction; /comments/1/reaction/evidences`
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta `UniProtKB:A9WBQ9`	enables Current J-10-fl HAMAP assignment for the joint alpha/beta carboxyltransferase function; neither subunit is asserted independently sufficient. The 2024 paper reverses locus/chain names, so it is not direct exact-accession assay evidence here.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	UniProtKB:A9WBQ9 Source assertion: The current catalytic annotation assigns the protein to Rhea 54728, transferring carboxyl from protein-bound carboxybiotin to acetyl-CoA. This is a HAMAP/inferred annotation, not a direct assay of this exact accession. Source annotation evidence: ECO:0000255. Source location `/comments/0/texts; /comments/1/reaction; /comments/1/reaction/evidences`
malonyl CoA reductase `MetaCyc:CPLX-7550`	catalyzes Bifunctional MCR from OK-70-fl. The native species-level complex is supported by the purified enzyme; its current J-10-fl component cross-reference is not equated to the OK-70-fl sequence. Physiological metal identity was not fixed.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	PMID:11948153 Source assertion: Purified OK-70-fl MCR reduces malonyl-CoA with two NADPH via a freely trapped malonate-semialdehyde intermediate; the two NADPH-dependent partial reactions are stated separately. Source location `Results sec23, transformation and stoichiometry; Discussion sec27` MetaCyc:CPLX-7550 Source assertion: The native complex describes bacterial bifunctional malonyl-CoA reductase and a two-subunit assembly. Source location `Protein[@frameid="CPLX-7550"]/comment; component`
malonyl CoA reductase `MetaCyc:CPLX-7550`	catalyzes Bifunctional MCR from OK-70-fl. The native species-level complex is supported by the purified enzyme; its current J-10-fl component cross-reference is not equated to the OK-70-fl sequence. Physiological metal identity was not fixed.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	PMID:11948153 Source assertion: Purified OK-70-fl MCR reduces malonyl-CoA with two NADPH via a freely trapped malonate-semialdehyde intermediate; the two NADPH-dependent partial reactions are stated separately. Source location `Results sec23, transformation and stoichiometry; Discussion sec27` MetaCyc:CPLX-7550 Source assertion: The native complex describes bacterial bifunctional malonyl-CoA reductase and a two-subunit assembly. Source location `Protein[@frameid="CPLX-7550"]/comment; component`
malonyl CoA reductase `MetaCyc:CPLX-7550`	has_part Two equivalent MCR subunits are supported by the purified OK-70-fl dimer estimate. Q6QQP7 is the separately submitted OK-70-fl sequence, not the distinct native J-10-fl A9WIU3 cross-reference.	Malonyl-CoA reductase `UniProtKB:Q6QQP7`	PMID:11948153 Source assertion: The purified OK-70-fl enzyme has an approximately 145-kDa subunit and 300-kDa native mass, supporting a homodimer; its N-terminal sequence matches the reported SDR-like open reading frame. Source location `Materials and Methods sec2; Results sec21; Discussion sec29` UniProtKB:Q6QQP7 Source assertion: The malonyl-CoA reductase sequence is attributed to OK-70-fl and EMBL AAS20429.1/AY530019; its source references link PMID11948153 and the January 2004 sequence submission. Source location `/references/0; /references/1; /uniProtKBCrossReferences[database=EMBL]; /sequence`
acetate--CoA ligase `UniProtKB:Q8VRG6`	enables Functional role of the OK-70-fl trifunctional PCS. Native assembly is three or four subunits; no unique stoichiometry or monomer sufficiency is asserted. The automated acetate-CoA-ligase name is not used to redefine its inspected pathway activity.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	MetaCyc:CPLX-7552 Source assertion: The native PCS complex catalyzes the three reactions that convert 3-hydroxypropanoate to propanoyl-CoA and describes a native assembly of three or four subunits. Source location `Protein[@frameid="CPLX-7552"]/comment; catalyzes` MetaCyc:MONOMER-13612 Source assertion: The native PCS subunit explicitly links both UniProt Q8VRG6 and A9WEU4 and belongs to the PCS complex. Source location `Protein[@frameid="MONOMER-13612"]/dblink[dblink-db="UNIPROT"]; component-of` UniProtKB:Q8VRG6 Source assertion: The sequence record attributes the protein to OK-70-fl and links the primary PCS paper PMID11821399 and nucleotide accession AF445079. Source location `/references; /uniProtKBCrossReferences[database=EMBL]; /sequence`
acetate--CoA ligase `UniProtKB:Q8VRG6`	enables Functional role of the OK-70-fl trifunctional PCS. Native assembly is three or four subunits; no unique stoichiometry or monomer sufficiency is asserted. The automated acetate-CoA-ligase name is not used to redefine its inspected pathway activity.	3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	MetaCyc:CPLX-7552 Source assertion: The native PCS complex catalyzes the three reactions that convert 3-hydroxypropanoate to propanoyl-CoA and describes a native assembly of three or four subunits. Source location `Protein[@frameid="CPLX-7552"]/comment; catalyzes` MetaCyc:MONOMER-13612 Source assertion: The native PCS subunit explicitly links both UniProt Q8VRG6 and A9WEU4 and belongs to the PCS complex. Source location `Protein[@frameid="MONOMER-13612"]/dblink[dblink-db="UNIPROT"]; component-of` UniProtKB:Q8VRG6 Source assertion: The sequence record attributes the protein to OK-70-fl and links the primary PCS paper PMID11821399 and nucleotide accession AF445079. Source location `/references; /uniProtKBCrossReferences[database=EMBL]; /sequence`
acetate--CoA ligase `UniProtKB:Q8VRG6`	enables Functional role of the OK-70-fl trifunctional PCS. Native assembly is three or four subunits; no unique stoichiometry or monomer sufficiency is asserted. The automated acetate-CoA-ligase name is not used to redefine its inspected pathway activity.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	MetaCyc:CPLX-7552 Source assertion: The native PCS complex catalyzes the three reactions that convert 3-hydroxypropanoate to propanoyl-CoA and describes a native assembly of three or four subunits. Source location `Protein[@frameid="CPLX-7552"]/comment; catalyzes` MetaCyc:MONOMER-13612 Source assertion: The native PCS subunit explicitly links both UniProt Q8VRG6 and A9WEU4 and belongs to the PCS complex. Source location `Protein[@frameid="MONOMER-13612"]/dblink[dblink-db="UNIPROT"]; component-of` UniProtKB:Q8VRG6 Source assertion: The sequence record attributes the protein to OK-70-fl and links the primary PCS paper PMID11821399 and nucleotide accession AF445079. Source location `/references; /uniProtKBCrossReferences[database=EMBL]; /sequence`
succinyl CoA:L-malate CoA transferase `MetaCyc:CPLX-7546`	catalyzes The purified and coexpressed OK-70-fl SmtAB complex transfers CoA from succinyl-CoA to (S)-malate. Magnesium in the coupled assay serves the downstream lyase, not SmtAB.	succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	PMID:16547052 Source assertion: Coexpression of SmtA and SmtB yielded succinyl-CoA:L-malate CoA transferase; purified enzyme forms malyl-CoA and succinate. Neither isolated subunit was active, and mixing did not reconstitute activity. Source location `Results sec39–sec40; Table 3` MetaCyc:CPLX-7546 Source assertion: The native species-level complex is succinyl CoA:L-malate CoA transferase and comprises six copies of its heterodimer. Source location `Protein[@frameid="CPLX-7546"]/common-name; component`
succinyl CoA:L-malate CoA transferase `MetaCyc:CPLX-7546`	has_part Six alpha/beta heterodimers in the source model; primary recombinant mass 550 +/- 50 kDa best fits (alpha beta)6. This is 12 polypeptide chains, not six identical subunits.	succinyl CoA:L-malate CoA transferase dimer `MetaCyc:CPLX-7548`	MetaCyc:CPLX-7546 Source assertion: The complex lists CPLX-7548 with component coefficient 6. Source location `Protein[@frameid="CPLX-7546"]/component` PMID:16547052 Source assertion: Gel filtration gave 550 +/- 50 kDa for the recombinant complex, best fitting six alpha/beta heterodimers; both chains are jointly required. Source location `Results sec39–sec40; Table 3`
succinyl CoA:L-malate CoA transferase dimer `MetaCyc:CPLX-7548`	has_part One selected OK-70-fl chain per alpha/beta heterodimer. Its identity comes from the exact primary-linked sequence record, not an equivalence to the native J-10-fl cross-reference.	Succinyl-CoA--L-malate CoA-transferase alpha subunit `UniProtKB:Q1KLK1`	UniProtKB:Q1KLK1 Source assertion: This Smt subunit is assigned to DSM636/OK-70-fl by its PMID16547052 sequence reference, and the experimentally supported subunit annotation describes six alpha/beta heterodimers. Source annotation evidence: ECO:0000269. Source location `/references/0; /comments/4/texts; /sequence` PMID:16547052 Source assertion: The experiment cloned and coexpressed the two OK-70-fl Smt chains; their subunit masses and N-terminal sequences identify the alpha/beta transferase. Source location `Methods sec2, sec14–sec16; Results sec38–sec40`
succinyl CoA:L-malate CoA transferase dimer `MetaCyc:CPLX-7548`	has_part One selected OK-70-fl chain per alpha/beta heterodimer. Its identity comes from the exact primary-linked sequence record, not an equivalence to the native J-10-fl cross-reference.	Succinyl-CoA--L-malate CoA-transferase beta subunit `UniProtKB:Q1KLK0`	UniProtKB:Q1KLK0 Source assertion: This Smt subunit is assigned to DSM636/OK-70-fl by its PMID16547052 sequence reference, and the experimentally supported subunit annotation describes six alpha/beta heterodimers. Source annotation evidence: ECO:0000269. Source location `/references/0; /comments/4/texts; /sequence` PMID:16547052 Source assertion: The experiment cloned and coexpressed the two OK-70-fl Smt chains; their subunit masses and N-terminal sequences identify the alpha/beta transferase. Source location `Methods sec2, sec14–sec16; Results sec38–sec40`
succinate dehydrogenase `UniProtKB:A9WDT7`	enables J-10-fl annotated SDH catalytic component; its ARBA inference does not establish an exact assayed OK-70-fl protein, a complete isolated enzyme, or the physiological quinone species.	a quinone + succinate => fumarate + a quinol `RHEA:40524`	UniProtKB:A9WDT7 Source assertion: The catalytic annotation assigns the generic quinone-dependent succinate-to-fumarate reaction Rhea40523 with ARBA evidence. Source annotation evidence: ECO:0000256. Source location `/comments/0/reaction; /comments/0/reaction/evidences`
L-malyl CoA lyase `MetaCyc:CPLX-7549`	catalyzes Hexameric OK-70-fl Mcl cleaves (S)-malyl-CoA to acetyl-CoA and glyoxylate. Its other two reactions belong to the separate glyoxylate-assimilation cycle.	(S)-malyl-CoA => glyoxylate + acetyl-CoA `RHEA:16630`	UniProtKB:S5N020 Source assertion: The directly supported first catalytic activity is Rhea16629, cleavage of (S)-malyl-CoA to glyoxylate and acetyl-CoA. Source annotation evidence: ECO:0000269. Source location `/comments/0/texts; /comments/1/reaction` PMID:12374834 Source assertion: Purified and recombinant OK-70-fl L-malyl-CoA lyase catalyze the reversible malyl-CoA cleavage reaction that regenerates acetyl-CoA in the first cycle. Source location `Results sec28; Figure 1; Methods sec2 and sec12`
L-malyl CoA lyase `MetaCyc:CPLX-7549`	has_part Six identical Mcl chains. The exact S5N020 sequence equals native-mapped A9WC35, but strain names remain distinct; 2013 directly studies the OK-70-fl construct and supports the hexamer by solution and structural measurements.	Malyl-CoA/beta-methylmalyl-CoA/citramalyl-CoA lyase `UniProtKB:S5N020`	MetaCyc:CPLX-7549 Source assertion: The native Mcl complex lists six copies of MONOMER-13604. Source location `Protein[@frameid="CPLX-7549"]/component` MetaCyc:MONOMER-13604 Source assertion: The native Mcl subunit explicitly links UniProt A9WC35. Source location `Protein[@frameid="MONOMER-13604"]/dblink[dblink-db="UNIPROT"]` PMID:24206647 Source assertion: OK-70-fl Mcl (AGR55786; Table 1) eluted at 228 +/- 15 kDa and crystallized as a hexamer; the structure is a dimer of trimers with active-site contributions from neighboring chains. Source location `Table 1; Results sec6 and sec9; Methods sec18` UniProtKB:A9WC35 Source assertion: The current native-linked J-10-fl entry supplies its 348-residue Mcl sequence as the independent comparison source. Source location `/organism; /sequence` UniProtKB:S5N020 Source assertion: The exact OK-70-fl sequence record maps AGR55786.1 and describes a homohexamer, a dimer of trimers, with experimental evidence. Source annotation evidence: ECO:0000269. Source location `/references/0–3; /uniProtKBCrossReferences[database=EMBL]; /sequence; /comments/7/texts`
biotin carboxylase `UniProtKB:A9W9X0`	has_cofactor Evidence tier and protein scope are retained; chemical identity denotes the cofactor rather than an extra consumed substrate.	biotinate `CHEBI:57586`	UniProtKB:A9W9X0 Source assertion: The current biotin-carboxylase annotation specifies biotin as cofactor with ARBA evidence; in the selected mechanism its covalently attached carrier states are explicit. Source annotation evidence: ECO:0000256. Source location `/comments/0/cofactors`
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta `UniProtKB:A9WBQ9`	has_cofactor Evidence tier and protein scope are retained; chemical identity denotes the cofactor rather than an extra consumed substrate.	zinc(2+) `CHEBI:29105`	UniProtKB:A9WBQ9 Source assertion: The current beta-chain annotation assigns one zinc ion per subunit by HAMAP; this is not a directly measured 2024 J-10-fl metal requirement. Source annotation evidence: ECO:0000255. Source location `/comments/2/cofactors; /comments/2/note`
succinate dehydrogenase `UniProtKB:A9WDT7`	has_cofactor Evidence tier and protein scope are retained; chemical identity denotes the cofactor rather than an extra consumed substrate.	FAD(3-) `CHEBI:57692`	UniProtKB:A9WDT7 Source assertion: The current SDH annotation assigns FAD as cofactor with ARBA evidence. Source annotation evidence: ECO:0000256. Source location `/comments/1/cofactors`
L-malyl CoA lyase `MetaCyc:CPLX-7549`	has_cofactor Alternative divalent-metal support, not a requirement to bind both metals simultaneously.	magnesium(2+) `CHEBI:18420`	UniProtKB:S5N020 Source assertion: The Mcl cofactor annotation includes magnesium as a divalent cofactor with experimental evidence. Source annotation evidence: ECO:0000269. Source location `/comments/4/cofactors; /comments/4/note` PMID:12374834 Source assertion: The purified lyase requires divalent metal; EDTA-treated enzyme is restored by Mg2+, while Mn2+ gives partial activity in the tested preparation. Source location `Results sec28, metal-dependence experiment`
L-malyl CoA lyase `MetaCyc:CPLX-7549`	has_cofactor Alternative divalent-metal support, not a requirement to bind both metals simultaneously.	manganese(2+) `CHEBI:29035`	UniProtKB:S5N020 Source assertion: The Mcl cofactor annotation includes manganese as a divalent cofactor with experimental evidence. Source annotation evidence: ECO:0000269. Source location `/comments/4/cofactors; /comments/4/note` PMID:12374834 Source assertion: The purified lyase requires divalent metal; EDTA-treated enzyme is restored by Mg2+, while Mn2+ gives partial activity in the tested preparation. Source location `Results sec28, metal-dependence experiment`
acetate--CoA ligase `UniProtKB:Q8VRG6`	has_cofactor Assayed potassium dependence of the OK-70-fl PCS preparation; partial substitution by other monovalent ions does not imply universal ion equivalence.	potassium(1+) `CHEBI:29103`	PMID:11821399 Source assertion: Potassium omission and titration establish PCS potassium dependence; related monovalent ions partially substitute. Source location `Results, Catalytic Properties of Propionyl-CoA Synthase; Methods, Characterization of the Enzyme`
acetate--CoA ligase `UniProtKB:Q8VRG6`	has_cofactor Magnesium is the experimentally reported PCS cocatalyst in MgATP-dependent assays; this does not assert a separately isolated metal-binding site.	magnesium(2+) `CHEBI:18420`	PMID:11821399 Source assertion: The authors tested magnesium dependence by omission and add-back and list magnesium as a cocatalyst. Source location `Methods, Characterization of the Enzyme; Table III, Cocatalysts`
CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	provides_input_for The shared chemical state is CHEBI:17544; this asserts material continuity, not substrate channeling or compulsory kinetic order.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:10750 Source assertion: Directed reaction 10750 produces CHEBI:17544. Source location `http://rdf.rhea-db.org/10750 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:13502 Source assertion: Directed reaction 13502 consumes CHEBI:17544. Source location `http://rdf.rhea-db.org/13502 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	provides_input_for The shared chemical state is CHEBI:17544; this asserts material continuity, not substrate channeling or compulsory kinetic order.	propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	RHEA:10750 Source assertion: Directed reaction 10750 produces CHEBI:17544. Source location `http://rdf.rhea-db.org/10750 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:23721 Source assertion: Directed reaction 23721 consumes CHEBI:17544. Source location `http://rdf.rhea-db.org/23721 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	provides_input_for The shared chemical state is RHEA-COMP:10506; this asserts material continuity, not substrate channeling or compulsory kinetic order.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	RHEA:13502 Source assertion: Directed reaction 13502 produces RHEA-COMP:10506. Source location `http://rdf.rhea-db.org/13502 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:54729 Source assertion: Directed reaction 54729 consumes RHEA-COMP:10506. Source location `http://rdf.rhea-db.org/54729 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	provides_input_for The shared chemical state is RHEA-COMP:10505; this asserts material continuity, not substrate channeling or compulsory kinetic order.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:54729 Source assertion: Directed reaction 54729 produces RHEA-COMP:10505. Source location `http://rdf.rhea-db.org/54729 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:13502 Source assertion: Directed reaction 13502 consumes RHEA-COMP:10505. Source location `http://rdf.rhea-db.org/13502 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	provides_input_for The shared chemical state is CHEBI:57384; this asserts material continuity, not substrate channeling or compulsory kinetic order.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	RHEA:54729 Source assertion: Directed reaction 54729 produces CHEBI:57384. Source location `http://rdf.rhea-db.org/54729 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:26448 Source assertion: Directed reaction 26448 consumes CHEBI:57384. Source location `http://rdf.rhea-db.org/26448 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	provides_input_for The shared chemical state is CHEBI:33190; this asserts material continuity, not substrate channeling or compulsory kinetic order.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	RHEA:26448 Source assertion: Directed reaction 26448 produces CHEBI:33190. Source location `http://rdf.rhea-db.org/26448 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:26440 Source assertion: Directed reaction 26440 consumes CHEBI:33190. Source location `http://rdf.rhea-db.org/26440 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	provides_input_for The shared chemical state is CHEBI:16510; this asserts material continuity, not substrate channeling or compulsory kinetic order.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	RHEA:26440 Source assertion: Directed reaction 26440 produces CHEBI:16510. Source location `http://rdf.rhea-db.org/26440 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:26535 Source assertion: Directed reaction 26535 consumes CHEBI:16510. Source location `http://rdf.rhea-db.org/26535 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	provides_input_for The shared chemical state is CHEBI:58528; this asserts material continuity, not substrate channeling or compulsory kinetic order.	3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	RHEA:26535 Source assertion: Directed reaction 26535 produces CHEBI:58528. Source location `http://rdf.rhea-db.org/26535 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:26519 Source assertion: Directed reaction 26519 consumes CHEBI:58528. Source location `http://rdf.rhea-db.org/26519 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	provides_input_for The shared chemical state is CHEBI:57367; this asserts material continuity, not substrate channeling or compulsory kinetic order.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	RHEA:26519 Source assertion: Directed reaction 26519 produces CHEBI:57367. Source location `http://rdf.rhea-db.org/26519 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:26456 Source assertion: Directed reaction 26456 consumes CHEBI:57367. Source location `http://rdf.rhea-db.org/26456 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	provides_input_for The shared chemical state is CHEBI:57392; this asserts material continuity, not substrate channeling or compulsory kinetic order.	propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	RHEA:26456 Source assertion: Directed reaction 26456 produces CHEBI:57392. Source location `http://rdf.rhea-db.org/26456 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:23721 Source assertion: Directed reaction 23721 consumes CHEBI:57392. Source location `http://rdf.rhea-db.org/23721 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) `RHEA:23721`	provides_input_for The shared chemical state is CHEBI:57327; this asserts material continuity, not substrate channeling or compulsory kinetic order.	(S)-methylmalonyl-CoA => (R)-methylmalonyl-CoA `RHEA:20555`	RHEA:23721 Source assertion: Directed reaction 23721 produces CHEBI:57327. Source location `http://rdf.rhea-db.org/23721 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:20555 Source assertion: Directed reaction 20555 consumes CHEBI:57327. Source location `http://rdf.rhea-db.org/20555 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
(S)-methylmalonyl-CoA => (R)-methylmalonyl-CoA `RHEA:20555`	provides_input_for The shared chemical state is CHEBI:57326; this asserts material continuity, not substrate channeling or compulsory kinetic order.	(R)-methylmalonyl-CoA => succinyl-CoA `RHEA:22889`	RHEA:20555 Source assertion: Directed reaction 20555 produces CHEBI:57326. Source location `http://rdf.rhea-db.org/20555 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:22889 Source assertion: Directed reaction 22889 consumes CHEBI:57326. Source location `http://rdf.rhea-db.org/22889 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
(R)-methylmalonyl-CoA => succinyl-CoA `RHEA:22889`	provides_input_for The shared chemical state is CHEBI:57292; this asserts material continuity, not substrate channeling or compulsory kinetic order.	succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	RHEA:22889 Source assertion: Directed reaction 22889 produces CHEBI:57292. Source location `http://rdf.rhea-db.org/22889 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:38256 Source assertion: Directed reaction 38256 consumes CHEBI:57292. Source location `http://rdf.rhea-db.org/38256 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	provides_input_for The shared chemical state is CHEBI:30031; this asserts material continuity, not substrate channeling or compulsory kinetic order.	a quinone + succinate => fumarate + a quinol `RHEA:40524`	RHEA:38256 Source assertion: Directed reaction 38256 produces CHEBI:30031. Source location `http://rdf.rhea-db.org/38256 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:40524 Source assertion: Directed reaction 40524 consumes CHEBI:30031. Source location `http://rdf.rhea-db.org/40524 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
a quinone + succinate => fumarate + a quinol `RHEA:40524`	provides_input_for The shared chemical state is CHEBI:29806; this asserts material continuity, not substrate channeling or compulsory kinetic order.	fumarate + H2O => (S)-malate `RHEA:12462`	RHEA:40524 Source assertion: Directed reaction 40524 produces CHEBI:29806. Source location `http://rdf.rhea-db.org/40524 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:12462 Source assertion: Directed reaction 12462 consumes CHEBI:29806. Source location `http://rdf.rhea-db.org/12462 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
fumarate + H2O => (S)-malate `RHEA:12462`	provides_input_for The shared chemical state is CHEBI:15589; this asserts material continuity, not substrate channeling or compulsory kinetic order.	succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	RHEA:12462 Source assertion: Directed reaction 12462 produces CHEBI:15589. Source location `http://rdf.rhea-db.org/12462 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:38256 Source assertion: Directed reaction 38256 consumes CHEBI:15589. Source location `http://rdf.rhea-db.org/38256 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate `RHEA:38256`	provides_input_for The shared chemical state is CHEBI:57317; this asserts material continuity, not substrate channeling or compulsory kinetic order.	(S)-malyl-CoA => glyoxylate + acetyl-CoA `RHEA:16630`	RHEA:38256 Source assertion: Directed reaction 38256 produces CHEBI:57317. Source location `http://rdf.rhea-db.org/38256 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:16630 Source assertion: Directed reaction 16630 consumes CHEBI:57317. Source location `http://rdf.rhea-db.org/16630 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`
(S)-malyl-CoA => glyoxylate + acetyl-CoA `RHEA:16630`	provides_input_for The shared chemical state is CHEBI:57288; this asserts material continuity, not substrate channeling or compulsory kinetic order.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	RHEA:16630 Source assertion: Directed reaction 16630 produces CHEBI:57288. Source location `http://rdf.rhea-db.org/16630 rh:products; corresponding side rh:contains1 / rh:compound / rh:accession` RHEA:54729 Source assertion: Directed reaction 54729 consumes CHEBI:57288. Source location `http://rdf.rhea-db.org/54729 rh:substrates; corresponding side rh:contains1 / rh:compound / rh:accession`

References

MetaCyc:PWY-5743 3-hydroxypropanoate cycle (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
GO:0043427 carbon fixation by 3-hydroxypropionate cycle (source version: Complete original QuickGO term JSON; retrieved 2026-10-11; active definition independently compared to pinned GO 2026-07-26)
PMID:11418572 Autotrophic CO2 Fixation by Chloroflexus aurantiacus: Study of Glyoxylate Formation and Assimilation via the 3-Hydroxypropionate Cycle (source version: Complete original PMC HTML body; retrieved 2026-10-11; assay and strain scope retained at edge level)
PMID:11948153 Malonyl-Coenzyme A Reductase from Chloroflexus aurantiacus, a Key Enzyme of the 3-Hydroxypropionate Cycle for Autotrophic CO2 Fixation (source version: Complete original PMC HTML body; retrieved 2026-10-11; assay and strain scope retained at edge level)
PMID:12374834 l-Malyl-Coenzyme A Lyase/β-Methylmalyl-Coenzyme A Lyase from Chloroflexus aurantiacus, a Bifunctional Enzyme Involved in Autotrophic CO2 Fixation (source version: Complete original PMC HTML body; retrieved 2026-10-11; assay and strain scope retained at edge level)
PMID:16547052 Properties of Succinyl-Coenzyme A:l-Malate Coenzyme A Transferase and Its Role in the Autotrophic 3-Hydroxypropionate Cycle of Chloroflexus aurantiacus (source version: Complete original PMC HTML body; retrieved 2026-10-11; assay and strain scope retained at edge level)
PMID:19955419 Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus (source version: Complete original PMC HTML body; retrieved 2026-10-11; assay and strain scope retained at edge level)
PMID:38572988 Chloroflexus aurantiacus acetyl-CoA carboxylase evolves fused biotin carboxylase and biotin carboxyl carrier protein to complete carboxylation activity (source version: Complete original PMC HTML body; retrieved 2026-10-11; assay and strain scope retained at edge level)
PMID:24206647 The crystal structures of the tri-functional Chloroflexus aurantiacus and bi-functional Rhodobacter sphaeroides malyl-CoA lyases and comparison with CitE-like superfamily enzymes and malate synthases (source version: Complete original PMC HTML body; retrieved 2026-10-11; assay and strain scope retained at edge level)
PMID:9973333 Presence of Acetyl Coenzyme A (CoA) Carboxylase and Propionyl-CoA Carboxylase in Autotrophic Crenarchaeota and Indication for Operation of a 3-Hydroxypropionate Cycle in Autotrophic Carbon Fixation (source version: Complete original PMC HTML body; retrieved 2026-10-11; assay and strain scope retained at edge level)
RHEA:10750 CO2 + H2O => hydrogencarbonate + H(+) (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:RXN0-5224 RXN0-5224 (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:13502 N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:BIOTIN-CARBOXYL-RXN BIOTIN-CARBOXYL-RXN (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:54729 N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:RXN0-5055 RXN0-5055 (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:26448 malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:RXN-8963 RXN-8963 (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:26440 3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:RXN-8974 RXN-8974 (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:26535 3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:RXN-9086 RXN-9086 (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:26519 3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:RXN-6383 RXN-6383 (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:26456 acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:RXN-9087 RXN-9087 (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:23721 propanoyl-CoA + hydrogencarbonate + ATP => (S)-methylmalonyl-CoA + ADP + phosphate + H(+) (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:PROPIONYL-COA-CARBOXY-RXN PROPIONYL-COA-CARBOXY-RXN (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:20555 (S)-methylmalonyl-CoA => (R)-methylmalonyl-CoA (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:METHYLMALONYL-COA-EPIM-RXN METHYLMALONYL-COA-EPIM-RXN (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:22889 (R)-methylmalonyl-CoA => succinyl-CoA (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:METHYLMALONYL-COA-MUT-RXN METHYLMALONYL-COA-MUT-RXN (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:38256 succinyl-CoA + (S)-malate => (S)-malyl-CoA + succinate (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:RXN-8964 RXN-8964 (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
RHEA:40524 a quinone + succinate => fumarate + a quinol (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
RHEA:12462 fumarate + H2O => (S)-malate (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
RHEA:16630 (S)-malyl-CoA => glyoxylate + acetyl-CoA (source version: Rhea release 142, 2026-09-02; SHA256 covers the complete original compressed RDF gzip, not an issuing HTML page)
MetaCyc:MALYL-COA-LYASE-RXN MALYL-COA-LYASE-RXN (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
UniProtKB:A9W9X0 biotin carboxylase (source version: UniProtKB 2026_03, 02-September-2026; complete original JSON response; retrieved 2026-10-11)
UniProtKB:A9WBQ8 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (source version: UniProtKB 2026_03, 02-September-2026; complete original JSON response; retrieved 2026-10-11)
UniProtKB:A9WBQ9 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (source version: UniProtKB 2026_03, 02-September-2026; complete original JSON response; retrieved 2026-10-11)
UniProtKB:A9WDT7 succinate dehydrogenase (source version: UniProtKB 2026_03, 02-September-2026; complete original JSON response; retrieved 2026-10-11)
UniProtKB:Q6QQP7 Malonyl-CoA reductase (source version: UniProtKB 2026_03, 02-September-2026; complete original JSON response; retrieved 2026-10-11)
UniProtKB:S5N020 Malyl-CoA/beta-methylmalyl-CoA/citramalyl-CoA lyase (source version: UniProtKB 2026_03, 02-September-2026; complete original JSON response; retrieved 2026-10-11)
UniProtKB:Q1KLK1 Succinyl-CoA--L-malate CoA-transferase alpha subunit (source version: UniProtKB 2026_03, 02-September-2026; complete original JSON response; retrieved 2026-10-11)
UniProtKB:Q1KLK0 Succinyl-CoA--L-malate CoA-transferase beta subunit (source version: UniProtKB 2026_03, 02-September-2026; complete original JSON response; retrieved 2026-10-11)
UniProtKB:Q8VRG6 acetate--CoA ligase (source version: UniProtKB 2026_03, 02-September-2026; complete original JSON response; retrieved 2026-10-11)
UniProtKB:A9WC35 Malyl-CoA lyase (source version: UniProtKB 2026_03; complete original JSON response; retrieved 2026-10-11; comparison source, not selected protein identity)
MetaCyc:CPLX-7550 malonyl CoA reductase (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
MetaCyc:CPLX-7549 L-malyl CoA lyase (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
MetaCyc:CPLX-7546 succinyl CoA:L-malate CoA transferase (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
MetaCyc:CPLX-7548 succinyl CoA:L-malate CoA transferase dimer (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
MetaCyc:MONOMER-13606 malonyl CoA reductase subunit (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
MetaCyc:MONOMER-13604 L-malyl CoA lyase subunit (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
MetaCyc:MONOMER-13599 succinyl CoA:L-malate CoA transferase α subunit (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
MetaCyc:MONOMER-13600 succinyl CoA:L-malate CoA transferase β subunit (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
MetaCyc:CPLX-7552 propionyl-CoA synthase (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
MetaCyc:MONOMER-13612 propionyl-CoA synthase subunit (source version: MetaCyc 22.5; complete original native XML body; retrieved 2026-10-11)
PMID:11821399 Propionyl-coenzyme A synthase from Chloroflexus aurantiacus, a key enzyme of the 3-hydroxypropionate cycle for autotrophic CO2 fixation (source version: Original 2002 paper inspected through ResearchGate web rendering on 2026-10-11; publisher/body downloads unavailable; no original-body SHA is asserted)

Curation history

2026-10-11T10:03:14Z codex
- CREATE — Add carbon fixation by 3-hydroxypropionate cycle
  Curated the complete first Chloroflexus aurantiacus 3-hydroxypropionate cycle as GO:0043427, including all 13 native objects plus experimentally supported succinate oxidation and fumarate hydration; exact strain, carrier and protein-identity limits remain explicit. Maintained record GO:0043427; creation bytes SHA256 34df3c9f0c29e7a61a3e687139c9dde7f98f48ad4ceec65b3d3168ea9f41776d. Original-source projections, replay and identifier decisions are in research/pathway_additions/2026-10-11-next-206/. Duplicate searches included ignored and hidden files. Independent review and full integration gates are separate subsequent checks, not claimed by this creation event.
Read this curation session