fructoselysine catabolic process

GO:1901281

The two-step fructoselysine catabolic route represented by the reviewed Escherichia coli K-12 proteins FrlD and FrlB converts fructoselysine to D-glucose 6-phosphate and L-lysine. FrlD uses ATP to form fructoselysine 6-phosphate; FrlB cleaves this intermediate with water. The graph selects the catabolic direction of the experimentally reversible FrlB reaction. Its scope is the core chemical conversion, with transport, psicoselysine epimerization, transcriptional regulation and alternative bacterial routes documented separately in the curation notes.

Pathway type
glycated amino acid catabolism
Organism scope

Read the source YAML

Pathway network

13 nodes · 14 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Pathway network: fructoselysine catabolic process 13 nodes · 14 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. CHEBI:61393 — consumes → RHEA:28379; Evidence: RHEA:28379consumes CHEBI:30616 — consumes → RHEA:28379; Evidence: RHEA:28379consumes RHEA:28379 — produces → CHEBI:61392; Evidence: RHEA:28379produces RHEA:28379 — produces → CHEBI:456216; Evidence: RHEA:28379produces RHEA:28379 — produces → CHEBI:15378; Evidence: RHEA:28379produces CHEBI:61392 — consumes → RHEA:28383; Evidence: RHEA:28383consumes CHEBI:15377 — consumes → RHEA:28383; Evidence: RHEA:28383consumes RHEA:28383 — produces → CHEBI:61548; Evidence: RHEA:28383produces RHEA:28383 — produces → CHEBI:32551; Evidence: RHEA:28383produces UniProtKB:P45543 — catalyzes → RHEA:28379; FrlD supplies the ATP-dependent kinase activity; UniProt annotates it as monomeric.; Evidence: UniProtKB:P45543, RHEA:28379, PMID:12147680, UniProtKB:P45543catalyzes UniProtKB:P0AC00 — catalyzes → RHEA:28383; FrlB supplies the deglycase activity and is annotated as homododecameric. The protein node denotes the gene product. The selected catabolic conversion also proceeds in reverse in vitro.; Evidence: UniProtKB:P0AC00, RHEA:28383, PMID:12147680, UniProtKB:P0AC00catalyzes RHEA:28379 — part_of → GO:1901281; The native UniPathway accession links the protein pathway assignment to the GO definition; the Rhea directional member supplies the reaction identity.; Evidence: UniProtKB:P45543, RHEA:28379, GO:1901281part of RHEA:28383 — part_of → GO:1901281; The native UniPathway accession links the protein pathway assignment to the GO definition; the Rhea directional member supplies the reaction identity.; Evidence: UniProtKB:P0AC00, RHEA:28383, GO:1901281part of RHEA:28379 — precedes → RHEA:28383; The kinase forms fructoselysine 6-phosphate, which is the deglycase substrate. Native pathway annotations specify steps 1/2 and 2/2.; Evidence: UniProtKB:P45543, UniProtKB:P0AC00, PMID:12147680precedes fructosyllysine(1+); CHEBI:61393; Declared type: small_molecule small molecule fructosyllysine(1+) ATP(4-); CHEBI:30616; Declared type: small_molecule small molecule ATP(4-) fructoselysine 6-phosphate(1-); CHEBI:61392; Declared type: small_molecule small molecule fructoselysine 6-phosphate(1-) ADP(3-); CHEBI:456216; Declared type: small_molecule small molecule ADP(3-) hydron; CHEBI:15378; Declared type: small_molecule small molecule hydron water; CHEBI:15377; Declared type: small_molecule small molecule water D-glucopyranose 6-phosphate(2-); CHEBI:61548; Declared type: small_molecule small molecule D-glucopyranose 6-phosphate(2-) L-lysinium(1+); CHEBI:32551; Declared type: small_molecule small molecule L-lysinium(1+) Fructoselysine 6-kinase; UniProtKB:P45543; Declared type: protein protein Fructoselysine 6-kinase Fructoselysine 6-phosphate deglycase; UniProtKB:P0AC00; Declared type: protein protein Fructoselysine 6-phosphate deglycase N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+); RHEA:28379; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D- fructosyl)-L-lysine + ADP + H(+) N(6)-(6-phospho-D-fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine; RHEA:28383; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity N(6)-(6-phospho-D- fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine fructoselysine catabolic process; GO:1901281; Declared type: pathway pathway fructoselysine catabolic process

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Escherichia coli K-12
NCBITaxon:83333		taxon
fructosyllysine(1+)
CHEBI:61393		small molecule
ATP(4-)
CHEBI:30616		small molecule
fructoselysine 6-phosphate(1-)
CHEBI:61392		small molecule
ADP(3-)
CHEBI:456216		small molecule
hydron
CHEBI:15378		small molecule
water
CHEBI:15377		small molecule
D-glucopyranose 6-phosphate(2-)
CHEBI:61548		small molecule
L-lysinium(1+)
CHEBI:32551		small molecule
Fructoselysine 6-kinase
UniProtKB:P45543		protein
Fructoselysine 6-phosphate deglycase
UniProtKB:P0AC00		protein
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		molecular activityleft to right
N(6)-(6-phospho-D-fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine
RHEA:28383		molecular activityleft to right

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
fructosyllysine(1+)
CHEBI:61393		consumes

N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379
  • RHEA:28379

    Source assertion: Rhea RHEA:28379 assigns CHEBI:61393 to its substrates, with coefficient 1 in rh:contains1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28379']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28378_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_28378_compound_6448']; rh:compound/rh:chebi
ATP(4-)
CHEBI:30616		consumes

N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379
  • RHEA:28379

    Source assertion: Rhea RHEA:28379 assigns CHEBI:30616 to its substrates, with coefficient 1 in rh:contains1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28379']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28378_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_28378_compound_6372']; rh:compound/rh:chebi
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		produces

fructoselysine 6-phosphate(1-)
CHEBI:61392
  • RHEA:28379

    Source assertion: Rhea RHEA:28379 assigns CHEBI:61392 to its products, with coefficient 1 in rh:contains1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28379']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28378_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_28378_compound_6447']; rh:compound/rh:chebi
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		produces

ADP(3-)
CHEBI:456216
  • RHEA:28379

    Source assertion: Rhea RHEA:28379 assigns CHEBI:456216 to its products, with coefficient 1 in rh:contains1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28379']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28378_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_28378_compound_2710']; rh:compound/rh:chebi
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		produces

hydron
CHEBI:15378
  • RHEA:28379

    Source assertion: Rhea RHEA:28379 assigns CHEBI:15378 to its products, with coefficient 1 in rh:contains1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28379']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28378_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_28378_compound_3249']; rh:compound/rh:chebi
fructoselysine 6-phosphate(1-)
CHEBI:61392		consumes

N(6)-(6-phospho-D-fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine
RHEA:28383
  • RHEA:28383

    Source assertion: Rhea RHEA:28383 assigns CHEBI:61392 to its substrates, with coefficient 1 in rh:contains1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28383']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28382_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_28382_compound_6447']; rh:compound/rh:chebi
water
CHEBI:15377		consumes

N(6)-(6-phospho-D-fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine
RHEA:28383
  • RHEA:28383

    Source assertion: Rhea RHEA:28383 assigns CHEBI:15377 to its substrates, with coefficient 1 in rh:contains1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28383']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28382_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_28382_compound_1283']; rh:compound/rh:chebi
N(6)-(6-phospho-D-fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine
RHEA:28383		produces

D-glucopyranose 6-phosphate(2-)
CHEBI:61548
  • RHEA:28383

    Source assertion: Rhea RHEA:28383 assigns CHEBI:61548 to its products, with coefficient 1 in rh:contains1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28383']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28382_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_28382_compound_6501']; rh:compound/rh:chebi
N(6)-(6-phospho-D-fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine
RHEA:28383		produces

L-lysinium(1+)
CHEBI:32551
  • RHEA:28383

    Source assertion: Rhea RHEA:28383 assigns CHEBI:32551 to its products, with coefficient 1 in rh:contains1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28383']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28382_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_28382_compound_3235']; rh:compound/rh:chebi
Fructoselysine 6-kinase
UniProtKB:P45543		catalyzes

FrlD supplies the ATP-dependent kinase activity; UniProt annotates it as monomeric.

N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379
  • UniProtKB:P45543

    Source assertion: UniProt assigns FrlD (P45543, taxon 83333) catalytic activity for RHEA:28378, with experimental annotation ECO:0000269 from PMID:12147680.

    Source locationprimaryAccession; organism.taxonId; comments[commentType='CATALYTIC ACTIVITY'].reaction[reactionCrossReferences.id='RHEA:28378'].evidences
  • RHEA:28379

    Source assertion: Rhea master RHEA:28378 has directional member RHEA:28379; its substrate/product assignments select the catabolic conversion represented here.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28378']/rh:directionalReaction[@rdf:resource='http://rdf.rhea-db.org/28379']; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28379']/rh:substrates and rh:products
  • PMID:12147680
    ATP-dependent phosphorylation of fructoselysine
    Source locationEurope PMC core resultList.result[id='12147680'].abstractText
  • UniProtKB:P45543

    Source assertion: UniProt SUBUNIT annotates P45543 as Monomer, with ECO:0000269 from PMID:12147680.

    Source locationcomments[commentType='SUBUNIT'].texts
Fructoselysine 6-phosphate deglycase
UniProtKB:P0AC00		catalyzes

FrlB supplies the deglycase activity and is annotated as homododecameric. The protein node denotes the gene product. The selected catabolic conversion also proceeds in reverse in vitro.

N(6)-(6-phospho-D-fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine
RHEA:28383
  • UniProtKB:P0AC00

    Source assertion: UniProt assigns FrlB (P0AC00, taxon 83333) catalytic activity for RHEA:28382, with experimental annotation ECO:0000269 from PMID:12147680.

    Source locationprimaryAccession; organism.taxonId; comments[commentType='CATALYTIC ACTIVITY'].reaction[reactionCrossReferences.id='RHEA:28382'].evidences
  • RHEA:28383

    Source assertion: Rhea master RHEA:28382 has directional member RHEA:28383; its substrate/product assignments select the catabolic conversion represented here.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28382']/rh:directionalReaction[@rdf:resource='http://rdf.rhea-db.org/28383']; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28383']/rh:substrates and rh:products
  • PMID:12147680
    reversible conversion of fructoselysine 6-phosphate and water
    Source locationEurope PMC core resultList.result[id='12147680'].abstractText
  • UniProtKB:P0AC00

    Source assertion: UniProt SUBUNIT annotates P0AC00 as Homododecamer, with ECO:0000269 from PMID:12147680.

    Source locationcomments[commentType='SUBUNIT'].texts
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		part_of

The native UniPathway accession links the protein pathway assignment to the GO definition; the Rhea directional member supplies the reaction identity.

GO:1901281
  • UniProtKB:P45543

    Source assertion: UniProt assigns P45543 to fructoselysine degradation, D-glucose 6-phosphate and lysine from fructoselysine, step 1/2 (ECO:0000305); its UniPathway cross-reference is UPA00784/UER00769.

    Source locationcomments[commentType='PATHWAY'].texts; uniProtKBCrossReferences[database='UniPathway',id='UPA00784'].properties[key='RectionId']
  • RHEA:28379

    Source assertion: Rhea master RHEA:28378 has directional member RHEA:28379; its substrate/product assignments select the catabolic conversion represented here.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28378']/rh:directionalReaction[@rdf:resource='http://rdf.rhea-db.org/28379']; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28379']/rh:substrates and rh:products
  • GO:1901281

    Source assertion: QuickGO defines GO:1901281 as fructoselysine breakdown and gives UniPathway UPA00784 as the definition cross-reference.

    Source locationresults[id='GO:1901281'].definition.text and definition.xrefs[dbCode='UniPathway',dbId='UPA00784']
N(6)-(6-phospho-D-fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine
RHEA:28383		part_of

The native UniPathway accession links the protein pathway assignment to the GO definition; the Rhea directional member supplies the reaction identity.

GO:1901281
  • UniProtKB:P0AC00

    Source assertion: UniProt assigns P0AC00 to fructoselysine degradation, D-glucose 6-phosphate and lysine from fructoselysine, step 2/2 (ECO:0000269 and ECO:0000305); its UniPathway cross-reference is UPA00784/UER00770.

    Source locationcomments[commentType='PATHWAY'].texts; uniProtKBCrossReferences[database='UniPathway',id='UPA00784'].properties[key='RectionId']
  • RHEA:28383

    Source assertion: Rhea master RHEA:28382 has directional member RHEA:28383; its substrate/product assignments select the catabolic conversion represented here.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/28382']/rh:directionalReaction[@rdf:resource='http://rdf.rhea-db.org/28383']; rdf:Description[@rdf:about='http://rdf.rhea-db.org/28383']/rh:substrates and rh:products
  • GO:1901281

    Source assertion: QuickGO defines GO:1901281 as fructoselysine breakdown and gives UniPathway UPA00784 as the definition cross-reference.

    Source locationresults[id='GO:1901281'].definition.text and definition.xrefs[dbCode='UniPathway',dbId='UPA00784']
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		precedes

The kinase forms fructoselysine 6-phosphate, which is the deglycase substrate. Native pathway annotations specify steps 1/2 and 2/2.

N(6)-(6-phospho-D-fructosyl)-L-lysine + H2O => D-glucose 6-phosphate + L-lysine
RHEA:28383
  • UniProtKB:P45543

    Source assertion: UniProt assigns P45543 to fructoselysine degradation, D-glucose 6-phosphate and lysine from fructoselysine, step 1/2 (ECO:0000305); its UniPathway cross-reference is UPA00784/UER00769.

    Source locationcomments[commentType='PATHWAY'].texts; uniProtKBCrossReferences[database='UniPathway',id='UPA00784'].properties[key='RectionId']
  • UniProtKB:P0AC00

    Source assertion: UniProt assigns P0AC00 to fructoselysine degradation, D-glucose 6-phosphate and lysine from fructoselysine, step 2/2 (ECO:0000269 and ECO:0000305); its UniPathway cross-reference is UPA00784/UER00770.

    Source locationcomments[commentType='PATHWAY'].texts; uniProtKBCrossReferences[database='UniPathway',id='UPA00784'].properties[key='RectionId']
  • PMID:12147680
    a delayed formation of glucose 6-phosphate
    Source locationEurope PMC core resultList.result[id='12147680'].abstractText

References

Curation history