Sporosarcina pasteurii ectoine biosynthetic gene cluster

MIBiG:BGC0000852

The ectABC operon from Sporosarcina pasteurii DSM 33 (ATCC 11859; named Bacillus pasteurii in the study) supports ectoine production. This cluster record preserves all three deposited gene products and the observed pathway output. Individual enzyme functions follow sequence-based assignments; detailed reaction, cofactor and localization claims are outside this record.

Pathway type
biosynthetic gene cluster
Organism scope

Read the source YAML

Pathway network

5 nodes · 4 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Pathway network: Sporosarcina pasteurii ectoine biosynthetic gene cluster 5 nodes · 4 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. NCBIProtein:AAK12083.1 — enables → MIBiG:BGC0000852; Gene-role assignment follows the deposited similarity annotation. Whole-operon functional support is recorded on the cluster-output edge; individual enzyme activity was not tested.; Evidence: NCBIProtein:AAK12083.1, MIBiG:BGC0000852enables NCBIProtein:AAK12084.1 — enables → MIBiG:BGC0000852; Gene-role assignment follows the deposited similarity annotation. Whole-operon functional support is recorded on the cluster-output edge; individual enzyme activity was not tested.; Evidence: NCBIProtein:AAK12084.1, MIBiG:BGC0000852enables NCBIProtein:AAK12085.1 — enables → MIBiG:BGC0000852; Gene-role assignment follows the deposited similarity annotation. Whole-operon functional support is recorded on the cluster-output edge; individual enzyme activity was not tested.; Evidence: NCBIProtein:AAK12085.1, MIBiG:BGC0000852enables MIBiG:BGC0000852 — produces → CHEBI:27592; Product detected after induced expression of the complete ectABC operon; native-promoter constructs did not produce detectable ectoine in the tested heterologous hosts.; Evidence: PMID:11823218produces EctA; NCBIProtein:AAK12083.1; Declared type: protein protein EctA EctB; NCBIProtein:AAK12084.1; Declared type: protein protein EctB EctC; NCBIProtein:AAK12085.1; Declared type: protein protein EctC ectoine; CHEBI:27592; Declared type: small_molecule small molecule ectoine Sporosarcina pasteurii ectABC cluster; MIBiG:BGC0000852; Declared type: pathway pathway Sporosarcina pasteurii ectABC cluster

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Sporosarcina pasteurii
NCBITaxon:1474		taxon
EctA
NCBIProtein:AAK12083.1		protein
EctB
NCBIProtein:AAK12084.1		protein
EctC
NCBIProtein:AAK12085.1		protein
ectoine
CHEBI:27592		small molecule

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
EctA
NCBIProtein:AAK12083.1		enables

Gene-role assignment follows the deposited similarity annotation. Whole-operon functional support is recorded on the cluster-output edge; individual enzyme activity was not tested.

Sporosarcina pasteurii ectABC cluster
MIBiG:BGC0000852
  • NCBIProtein:AAK12083.1

    Source assertion: AAK12083.1 is annotated as EctA, similar to acetyltransferase, and its CDS is assigned to ectA at AF316874.1:416..958. This is a similarity-based gene-product assignment.

    Source locationGBSet/GBSeq[GBSeq_accession-version='AAK12083.1']/GBSeq_feature-table/GBFeature[GBFeature_key='Protein' or GBFeature_key='CDS']/GBFeature_quals
  • MIBiG:BGC0000852

    Source assertion: BGC0000852 identifies AF316874.1 as its locus and ectoine as its product; locus boundaries are unspecified (zero values).

    Source location$.loci[0].accession; $.loci[0].location; $.compounds[0].name
EctB
NCBIProtein:AAK12084.1		enables

Gene-role assignment follows the deposited similarity annotation. Whole-operon functional support is recorded on the cluster-output edge; individual enzyme activity was not tested.

Sporosarcina pasteurii ectABC cluster
MIBiG:BGC0000852
  • NCBIProtein:AAK12084.1

    Source assertion: AAK12084.1 is annotated as EctB, similar to transaminase, and its CDS is assigned to ectB at AF316874.1:1010..2290. This is a similarity-based gene-product assignment.

    Source locationGBSet/GBSeq[GBSeq_accession-version='AAK12084.1']/GBSeq_feature-table/GBFeature[GBFeature_key='Protein' or GBFeature_key='CDS']/GBFeature_quals
  • MIBiG:BGC0000852

    Source assertion: BGC0000852 identifies AF316874.1 as its locus and ectoine as its product; locus boundaries are unspecified (zero values).

    Source location$.loci[0].accession; $.loci[0].location; $.compounds[0].name
EctC
NCBIProtein:AAK12085.1		enables

Gene-role assignment follows the deposited similarity annotation. Whole-operon functional support is recorded on the cluster-output edge; individual enzyme activity was not tested.

Sporosarcina pasteurii ectABC cluster
MIBiG:BGC0000852
  • NCBIProtein:AAK12085.1

    Source assertion: AAK12085.1 is annotated as EctC, similar to ectoine synthase, and its CDS is assigned to ectC at AF316874.1:2450..2854. This is a similarity-based gene-product assignment.

    Source locationGBSet/GBSeq[GBSeq_accession-version='AAK12085.1']/GBSeq_feature-table/GBFeature[GBFeature_key='Protein' or GBFeature_key='CDS']/GBFeature_quals
  • MIBiG:BGC0000852

    Source assertion: BGC0000852 identifies AF316874.1 as its locus and ectoine as its product; locus boundaries are unspecified (zero values).

    Source location$.loci[0].accession; $.loci[0].location; $.compounds[0].name
Sporosarcina pasteurii ectABC cluster
MIBiG:BGC0000852		produces

Product detected after induced expression of the complete ectABC operon; native-promoter constructs did not produce detectable ectoine in the tested heterologous hosts.

ectoine
CHEBI:27592
  • PMID:11823218
    their heterologous expression in B. subtilis led to the production of ectoine.

Biosynthetic gene clusters

References

Curation history