chorismate biosynthesis I

MetaCyc:ARO-PWY

Chorismate biosynthesis I converts D-erythrose 4-phosphate and phosphoenolpyruvate to chorismate through 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate, 3-dehydroquinate, 3-dehydroshikimate, shikimate, 3-phosphoshikimate, and 5-O-(1-carboxyvinyl)-3-phosphoshikimate.

Pathway type

amino acid biosynthesis

Organism scope

Escherichia coli NCBITaxon:562

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Pathway network

48 nodes · 66 asserted directed edges

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Escherichia coli `NCBITaxon:562`	taxon
3-deoxy-7-phosphoheptulonate synthase `EC:2.5.1.54`	molecular activity
3-dehydroquinate synthase `EC:4.2.3.4`	molecular activity
3-dehydroquinate dehydratase `EC:4.2.1.10`	molecular activity
shikimate dehydrogenase `EC:1.1.1.25`	molecular activity
shikimate kinase `EC:2.7.1.71`	molecular activity
3-phosphoshikimate 1-carboxyvinyltransferase `EC:2.5.1.19`	molecular activity
chorismate synthase `EC:4.2.3.5`	molecular activity
D-erythrose 4-phosphate `CHEBI:16897`	small molecule
phosphoenolpyruvate `CHEBI:58702`	small molecule
H2O `CHEBI:15377`	small molecule
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate `CHEBI:58394`	small molecule
phosphate `CHEBI:43474`	small molecule
3-dehydroquinate `CHEBI:32364`	small molecule
3-dehydroshikimate `CHEBI:16630`	small molecule
NADPH `CHEBI:57783`	small molecule
H(+) `CHEBI:15378`	small molecule
shikimate `CHEBI:36208`	small molecule
NADP(+) `CHEBI:58349`	small molecule
ATP `CHEBI:30616`	small molecule
3-phosphoshikimate `CHEBI:145989`	small molecule
ADP `CHEBI:456216`	small molecule
5-O-(1-carboxyvinyl)-3-phosphoshikimate `CHEBI:57701`	small molecule
chorismate `CHEBI:29748`	small molecule
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive `UniProtKB:P00887`	protein
cytoplasm `GO:0005737`	cellular component
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive `UniProtKB:P00888`	protein
L-tyrosine zwitterion `CHEBI:58315`	small molecule
divalent metal cation `CHEBI:60240`	cofactor
3-dehydroquinate dehydratase `UniProtKB:P05194`	protein
3-dehydroquinate synthase `UniProtKB:P07639`	protein
NAD(1-) `CHEBI:57540`	cofactor
cobalt(2+) `CHEBI:48828`	cofactor
zinc(2+) `CHEBI:29105`	cofactor
3-phosphoshikimate 1-carboxyvinyltransferase `UniProtKB:P0A6D3`	protein
Shikimate kinase 1 `UniProtKB:P0A6D7`	protein
magnesium(2+) `CHEBI:18420`	cofactor
Shikimate kinase 2 `UniProtKB:P0A6E1`	protein
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive `UniProtKB:P0AB91`	protein
Chorismate synthase `UniProtKB:P12008`	protein
FMNH2(2-) `CHEBI:57618`	cofactor
Shikimate dehydrogenase (NADP(+)) `UniProtKB:P15770`	protein
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	reaction	left to right
3-dehydroquinate synthase reaction `RHEA:21969`	reaction	left to right
3-dehydroquinate dehydratase reaction `RHEA:21097`	reaction	left to right
biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	reaction	right to left
shikimate kinase reaction `RHEA:13122`	reaction	left to right
3-phosphoshikimate 1-carboxyvinyltransferase reaction `RHEA:21257`	reaction	left to right
chorismate synthase reaction `RHEA:21021`	reaction	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
H2O `CHEBI:15377`	consumes	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	RHEA:14718 Source assertion: Rhea lists CHEBI:15377 on the substrates side of RHEA:14718 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14718']/rh:substrates; rh:contains/rh:compound/rh:accession`
D-erythrose 4-phosphate `CHEBI:16897`	consumes	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	RHEA:14718 Source assertion: Rhea lists CHEBI:16897 on the substrates side of RHEA:14718 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14718']/rh:substrates; rh:contains/rh:compound/rh:accession`
phosphoenolpyruvate `CHEBI:58702`	consumes	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	RHEA:14718 Source assertion: Rhea lists CHEBI:58702 on the substrates side of RHEA:14718 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14718']/rh:substrates; rh:contains/rh:compound/rh:accession`
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	produces	phosphate `CHEBI:43474`	RHEA:14718 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:14718 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14718']/rh:products; rh:contains/rh:compound/rh:accession`
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	produces	7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate `CHEBI:58394`	RHEA:14718 Source assertion: Rhea lists CHEBI:58394 on the products side of RHEA:14718 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14718']/rh:products; rh:contains/rh:compound/rh:accession`
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate `CHEBI:58394`	consumes	3-dehydroquinate synthase reaction `RHEA:21969`	RHEA:21969 Source assertion: Rhea lists CHEBI:58394 on the substrates side of RHEA:21969 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21969']/rh:substrates; rh:contains/rh:compound/rh:accession`
3-dehydroquinate synthase reaction `RHEA:21969`	produces	3-dehydroquinate `CHEBI:32364`	RHEA:21969 Source assertion: Rhea lists CHEBI:32364 on the products side of RHEA:21969 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21969']/rh:products; rh:contains/rh:compound/rh:accession`
3-dehydroquinate synthase reaction `RHEA:21969`	produces	phosphate `CHEBI:43474`	RHEA:21969 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:21969 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21969']/rh:products; rh:contains/rh:compound/rh:accession`
3-dehydroquinate `CHEBI:32364`	consumes	3-dehydroquinate dehydratase reaction `RHEA:21097`	RHEA:21097 Source assertion: Rhea lists CHEBI:32364 on the substrates side of RHEA:21097 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21097']/rh:substrates; rh:contains/rh:compound/rh:accession`
3-dehydroquinate dehydratase reaction `RHEA:21097`	produces	H2O `CHEBI:15377`	RHEA:21097 Source assertion: Rhea lists CHEBI:15377 on the products side of RHEA:21097 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21097']/rh:products; rh:contains/rh:compound/rh:accession`
3-dehydroquinate dehydratase reaction `RHEA:21097`	produces	3-dehydroshikimate `CHEBI:16630`	RHEA:21097 Source assertion: Rhea lists CHEBI:16630 on the products side of RHEA:21097 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21097']/rh:products; rh:contains/rh:compound/rh:accession`
H(+) `CHEBI:15378`	consumes	biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	RHEA:17739 Source assertion: Rhea lists CHEBI:15378 on the substrates side of RHEA:17739 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17739']/rh:substrates; rh:contains/rh:compound/rh:accession`
3-dehydroshikimate `CHEBI:16630`	consumes	biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	RHEA:17739 Source assertion: Rhea lists CHEBI:16630 on the substrates side of RHEA:17739 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17739']/rh:substrates; rh:contains/rh:compound/rh:accession`
NADPH `CHEBI:57783`	consumes	biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	RHEA:17739 Source assertion: Rhea lists CHEBI:57783 on the substrates side of RHEA:17739 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17739']/rh:substrates; rh:contains/rh:compound/rh:accession`
biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	produces	shikimate `CHEBI:36208`	RHEA:17739 Source assertion: Rhea lists CHEBI:36208 on the products side of RHEA:17739 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17739']/rh:products; rh:contains/rh:compound/rh:accession`
biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	produces	NADP(+) `CHEBI:58349`	RHEA:17739 Source assertion: Rhea lists CHEBI:58349 on the products side of RHEA:17739 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17739']/rh:products; rh:contains/rh:compound/rh:accession`
ATP `CHEBI:30616`	consumes	shikimate kinase reaction `RHEA:13122`	RHEA:13122 Source assertion: Rhea lists CHEBI:30616 on the substrates side of RHEA:13122 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/13122']/rh:substrates; rh:contains/rh:compound/rh:accession`
shikimate `CHEBI:36208`	consumes	shikimate kinase reaction `RHEA:13122`	RHEA:13122 Source assertion: Rhea lists CHEBI:36208 on the substrates side of RHEA:13122 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/13122']/rh:substrates; rh:contains/rh:compound/rh:accession`
shikimate kinase reaction `RHEA:13122`	produces	3-phosphoshikimate `CHEBI:145989`	RHEA:13122 Source assertion: Rhea lists CHEBI:145989 on the products side of RHEA:13122 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/13122']/rh:products; rh:contains/rh:compound/rh:accession`
shikimate kinase reaction `RHEA:13122`	produces	H(+) `CHEBI:15378`	RHEA:13122 Source assertion: Rhea lists CHEBI:15378 on the products side of RHEA:13122 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/13122']/rh:products; rh:contains/rh:compound/rh:accession`
shikimate kinase reaction `RHEA:13122`	produces	ADP `CHEBI:456216`	RHEA:13122 Source assertion: Rhea lists CHEBI:456216 on the products side of RHEA:13122 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/13122']/rh:products; rh:contains/rh:compound/rh:accession`
3-phosphoshikimate `CHEBI:145989`	consumes	3-phosphoshikimate 1-carboxyvinyltransferase reaction `RHEA:21257`	RHEA:21257 Source assertion: Rhea lists CHEBI:145989 on the substrates side of RHEA:21257 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21257']/rh:substrates; rh:contains/rh:compound/rh:accession`
phosphoenolpyruvate `CHEBI:58702`	consumes	3-phosphoshikimate 1-carboxyvinyltransferase reaction `RHEA:21257`	RHEA:21257 Source assertion: Rhea lists CHEBI:58702 on the substrates side of RHEA:21257 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21257']/rh:substrates; rh:contains/rh:compound/rh:accession`
3-phosphoshikimate 1-carboxyvinyltransferase reaction `RHEA:21257`	produces	phosphate `CHEBI:43474`	RHEA:21257 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:21257 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21257']/rh:products; rh:contains/rh:compound/rh:accession`
3-phosphoshikimate 1-carboxyvinyltransferase reaction `RHEA:21257`	produces	5-O-(1-carboxyvinyl)-3-phosphoshikimate `CHEBI:57701`	RHEA:21257 Source assertion: Rhea lists CHEBI:57701 on the products side of RHEA:21257 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21257']/rh:products; rh:contains/rh:compound/rh:accession`
5-O-(1-carboxyvinyl)-3-phosphoshikimate `CHEBI:57701`	consumes	chorismate synthase reaction `RHEA:21021`	RHEA:21021 Source assertion: Rhea lists CHEBI:57701 on the substrates side of RHEA:21021 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21021']/rh:substrates; rh:contains/rh:compound/rh:accession`
chorismate synthase reaction `RHEA:21021`	produces	chorismate `CHEBI:29748`	RHEA:21021 Source assertion: Rhea lists CHEBI:29748 on the products side of RHEA:21021 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21021']/rh:products; rh:contains/rh:compound/rh:accession`
chorismate synthase reaction `RHEA:21021`	produces	phosphate `CHEBI:43474`	RHEA:21021 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:21021 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/21021']/rh:products; rh:contains/rh:compound/rh:accession`
3-deoxy-7-phosphoheptulonate synthase `EC:2.5.1.54`	catalyzes	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	RHEA:14717 Source assertion: Rhea associates EC:2.5.1.54 with the reaction family containing RHEA:14718. Source location `rh:ec`
3-dehydroquinate synthase `EC:4.2.3.4`	catalyzes	3-dehydroquinate synthase reaction `RHEA:21969`	RHEA:21968 Source assertion: Rhea associates EC:4.2.3.4 with the reaction family containing RHEA:21969. Source location `rh:ec`
3-dehydroquinate dehydratase `EC:4.2.1.10`	catalyzes	3-dehydroquinate dehydratase reaction `RHEA:21097`	RHEA:21096 Source assertion: Rhea associates EC:4.2.1.10 with the reaction family containing RHEA:21097. Source location `rh:ec`
shikimate dehydrogenase `EC:1.1.1.25`	catalyzes	biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	RHEA:17737 Source assertion: Rhea associates EC:1.1.1.25 with the reaction family containing RHEA:17739. Source location `rh:ec`
shikimate kinase `EC:2.7.1.71`	catalyzes	shikimate kinase reaction `RHEA:13122`	RHEA:13121 Source assertion: Rhea associates EC:2.7.1.71 with the reaction family containing RHEA:13122. Source location `rh:ec`
3-phosphoshikimate 1-carboxyvinyltransferase `EC:2.5.1.19`	catalyzes	3-phosphoshikimate 1-carboxyvinyltransferase reaction `RHEA:21257`	RHEA:21256 Source assertion: Rhea associates EC:2.5.1.19 with the reaction family containing RHEA:21257. Source location `rh:ec`
chorismate synthase `EC:4.2.3.5`	catalyzes	chorismate synthase reaction `RHEA:21021`	RHEA:21020 Source assertion: Rhea associates EC:4.2.3.5 with the reaction family containing RHEA:21021. Source location `rh:ec`
shikimate kinase reaction `RHEA:13122`	precedes	3-phosphoshikimate 1-carboxyvinyltransferase reaction `RHEA:21257`	MetaCyc:ARO-PWY Source assertion: MetaCyc pathway ordering places RHEA:13122 before RHEA:21257 in this pathway. Shared intermediate: CHEBI:145989. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	precedes	3-dehydroquinate synthase reaction `RHEA:21969`	MetaCyc:ARO-PWY Source assertion: MetaCyc pathway ordering places RHEA:14718 before RHEA:21969 in this pathway. Shared intermediate: CHEBI:58394. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	precedes	shikimate kinase reaction `RHEA:13122`	MetaCyc:ARO-PWY Source assertion: MetaCyc pathway ordering places RHEA:17739 before RHEA:13122 in this pathway. Shared intermediate: CHEBI:36208. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
3-dehydroquinate dehydratase reaction `RHEA:21097`	precedes	biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	MetaCyc:ARO-PWY Source assertion: MetaCyc pathway ordering places RHEA:21097 before RHEA:17739 in this pathway. Shared intermediate: CHEBI:16630. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
3-phosphoshikimate 1-carboxyvinyltransferase reaction `RHEA:21257`	precedes	chorismate synthase reaction `RHEA:21021`	MetaCyc:ARO-PWY Source assertion: MetaCyc pathway ordering places RHEA:21257 before RHEA:21021 in this pathway. Shared intermediate: CHEBI:57701. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
3-dehydroquinate synthase reaction `RHEA:21969`	precedes	3-dehydroquinate dehydratase reaction `RHEA:21097`	MetaCyc:ARO-PWY Source assertion: MetaCyc pathway ordering places RHEA:21969 before RHEA:21097 in this pathway. Shared intermediate: CHEBI:32364. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive `UniProtKB:P00887`	enables	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	UniProtKB:P00887 Source assertion: UniProt annotates UniProtKB:P00887 with catalytic activity for the reaction family of RHEA:14718. Annotation evidence: curated source annotation; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive `UniProtKB:P00887`	located_in	cytoplasm `GO:0005737`	UniProtKB:P00887 Source assertion: UniProt locates UniProtKB:P00887 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000255. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive `UniProtKB:P00888`	enables	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	UniProtKB:P00888 Source assertion: UniProt annotates UniProtKB:P00888 with catalytic activity for the reaction family of RHEA:14718. Annotation evidence: curated source annotation; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
L-tyrosine zwitterion `CHEBI:58315`	inhibits	Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive `UniProtKB:P00888`	UniProtKB:P00888 Source assertion: UniProt activity-regulation annotation: Specifically feedback inhibited by tyrosine with 50% inhibition observed at 9 microM tyrosine, pH 7.0 Source location `comments[commentType='ACTIVITY REGULATION'].texts`
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive `UniProtKB:P00888`	located_in	cytoplasm `GO:0005737`	UniProtKB:P00888 Source assertion: UniProt locates UniProtKB:P00888 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000255. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive `UniProtKB:P00888`	has_cofactor	divalent metal cation `CHEBI:60240`	UniProtKB:P00888 Source assertion: UniProt lists CHEBI:60240 as a cofactor of UniProtKB:P00888 (evidence: curated annotation). Source location `comments[commentType='COFACTOR'].cofactors and note`
3-dehydroquinate dehydratase `UniProtKB:P05194`	enables	3-dehydroquinate dehydratase reaction `RHEA:21097`	UniProtKB:P05194 Source assertion: UniProt annotates UniProtKB:P05194 with catalytic activity for the reaction family of RHEA:21097. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
3-dehydroquinate synthase `UniProtKB:P07639`	enables	3-dehydroquinate synthase reaction `RHEA:21969`	UniProtKB:P07639 Source assertion: UniProt annotates UniProtKB:P07639 with catalytic activity for the reaction family of RHEA:21969. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
3-dehydroquinate synthase `UniProtKB:P07639`	located_in	cytoplasm `GO:0005737`	UniProtKB:P07639 Source assertion: UniProt locates UniProtKB:P07639 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000255. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
3-dehydroquinate synthase `UniProtKB:P07639`	has_cofactor	NAD(1-) `CHEBI:57540`	UniProtKB:P07639 Source assertion: UniProt lists CHEBI:57540 as a cofactor of UniProtKB:P07639 (evidence: ECO:0000255, ECO:0000269). Source location `comments[commentType='COFACTOR'].cofactors and note`
3-dehydroquinate synthase `UniProtKB:P07639`	has_cofactor	cobalt(2+) `CHEBI:48828`	UniProtKB:P07639 Source assertion: UniProt lists CHEBI:48828 as a cofactor of UniProtKB:P07639 (evidence: ECO:0000255, ECO:0000269). Binds 1 divalent metal cation per subunit. Displays higher activity with Co(2+), however, Zn(2+) may be the physiological metal cofactor. Source location `comments[commentType='COFACTOR'].cofactors and note`
3-dehydroquinate synthase `UniProtKB:P07639`	has_cofactor	zinc(2+) `CHEBI:29105`	UniProtKB:P07639 Source assertion: UniProt lists CHEBI:29105 as a cofactor of UniProtKB:P07639 (evidence: ECO:0000255, ECO:0000269). Binds 1 divalent metal cation per subunit. Displays higher activity with Co(2+), however, Zn(2+) may be the physiological metal cofactor. Source location `comments[commentType='COFACTOR'].cofactors and note`
3-phosphoshikimate 1-carboxyvinyltransferase `UniProtKB:P0A6D3`	enables	3-phosphoshikimate 1-carboxyvinyltransferase reaction `RHEA:21257`	UniProtKB:P0A6D3 Source assertion: UniProt annotates UniProtKB:P0A6D3 with catalytic activity for the reaction family of RHEA:21257. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
3-phosphoshikimate 1-carboxyvinyltransferase `UniProtKB:P0A6D3`	located_in	cytoplasm `GO:0005737`	UniProtKB:P0A6D3 Source assertion: UniProt locates UniProtKB:P0A6D3 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000255, ECO:0000305. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Shikimate kinase 1 `UniProtKB:P0A6D7`	enables	shikimate kinase reaction `RHEA:13122`	UniProtKB:P0A6D7 Source assertion: UniProt annotates UniProtKB:P0A6D7 with catalytic activity for the reaction family of RHEA:13122. Annotation evidence: curated source annotation; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Shikimate kinase 1 `UniProtKB:P0A6D7`	located_in	cytoplasm `GO:0005737`	UniProtKB:P0A6D7 Source assertion: UniProt locates UniProtKB:P0A6D7 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000305. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Shikimate kinase 1 `UniProtKB:P0A6D7`	has_cofactor	magnesium(2+) `CHEBI:18420`	UniProtKB:P0A6D7 Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:P0A6D7 (evidence: ECO:0000305). Binds 1 Mg(2+) ion per subunit. Source location `comments[commentType='COFACTOR'].cofactors and note`
Shikimate kinase 2 `UniProtKB:P0A6E1`	enables	shikimate kinase reaction `RHEA:13122`	UniProtKB:P0A6E1 Source assertion: UniProt annotates UniProtKB:P0A6E1 with catalytic activity for the reaction family of RHEA:13122. Annotation evidence: ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Shikimate kinase 2 `UniProtKB:P0A6E1`	located_in	cytoplasm `GO:0005737`	UniProtKB:P0A6E1 Source assertion: UniProt locates UniProtKB:P0A6E1 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000250. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Shikimate kinase 2 `UniProtKB:P0A6E1`	has_cofactor	magnesium(2+) `CHEBI:18420`	UniProtKB:P0A6E1 Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:P0A6E1 (evidence: ECO:0000269). Binds 1 Mg(2+) ion per subunit. Source location `comments[commentType='COFACTOR'].cofactors and note`
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive `UniProtKB:P0AB91`	enables	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction `RHEA:14718`	UniProtKB:P0AB91 Source assertion: UniProt annotates UniProtKB:P0AB91 with catalytic activity for the reaction family of RHEA:14718. Annotation evidence: curated source annotation; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive `UniProtKB:P0AB91`	located_in	cytoplasm `GO:0005737`	UniProtKB:P0AB91 Source assertion: UniProt locates UniProtKB:P0AB91 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000255. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Chorismate synthase `UniProtKB:P12008`	enables	chorismate synthase reaction `RHEA:21021`	UniProtKB:P12008 Source assertion: UniProt annotates UniProtKB:P12008 with catalytic activity for the reaction family of RHEA:21021. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Chorismate synthase `UniProtKB:P12008`	has_cofactor	FMNH2(2-) `CHEBI:57618`	UniProtKB:P12008 Source assertion: UniProt lists CHEBI:57618 as a cofactor of UniProtKB:P12008 (evidence: ECO:0000255, ECO:0000269). Reduced FMN (FMNH(2)). It can also use FAD, however FMN is the preferred cofactor. Source location `comments[commentType='COFACTOR'].cofactors and note`
Shikimate dehydrogenase (NADP(+)) `UniProtKB:P15770`	enables	biosynthetic shikimate dehydrogenase reaction `RHEA:17739`	UniProtKB:P15770 Source assertion: UniProt annotates UniProtKB:P15770 with catalytic activity for the reaction family of RHEA:17739. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`

References

MetaCyc:ARO-PWY MetaCyc chorismate biosynthesis I pathway (source version: 22.5)
RHEA:14717 Rhea 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase reaction (source version: 139)
RHEA:14718 Rhea 3-deoxy-D-arabino-heptulosonate 7-phosphate-forming direction (source version: 139)
RHEA:21968 Rhea 3-dehydroquinate synthase reaction (source version: 139)
RHEA:21969 Rhea 3-dehydroquinate-forming direction (source version: 139)
RHEA:21096 Rhea 3-dehydroquinate dehydratase reaction (source version: 139)
RHEA:21097 Rhea 3-dehydroshikimate-forming direction (source version: 139)
RHEA:17737 Rhea shikimate dehydrogenase reaction (source version: 139)
RHEA:17739 Rhea shikimate-forming direction (source version: 139)
RHEA:13121 Rhea shikimate kinase reaction (source version: 139)
RHEA:13122 Rhea 3-phosphoshikimate-forming direction (source version: 139)
RHEA:21256 Rhea 3-phosphoshikimate 1-carboxyvinyltransferase reaction (source version: 139)
RHEA:21257 Rhea 5-O-(1-carboxyvinyl)-3-phosphoshikimate-forming direction (source version: 139)
RHEA:21020 Rhea chorismate synthase reaction (source version: 139)
RHEA:21021 Rhea chorismate-forming direction (source version: 139)
UniProtKB:P00887 UniProtKB:P00887 authority record (source version: 2026_03)
UniProtKB:P00888 UniProtKB:P00888 authority record (source version: 2026_03)
UniProtKB:P05194 UniProtKB:P05194 authority record (source version: 2026_03)
UniProtKB:P07639 UniProtKB:P07639 authority record (source version: 2026_03)
UniProtKB:P0A6D3 UniProtKB:P0A6D3 authority record (source version: 2026_03)
UniProtKB:P0A6D7 UniProtKB:P0A6D7 authority record (source version: 2026_03)
UniProtKB:P0A6E1 UniProtKB:P0A6E1 authority record (source version: 2026_03)
UniProtKB:P0AB91 UniProtKB:P0AB91 authority record (source version: 2026_03)
UniProtKB:P12008 UniProtKB:P12008 authority record (source version: 2026_03)
UniProtKB:P15770 UniProtKB:P15770 authority record (source version: 2026_03)

Curation history

2026-10-05T06:25:52Z codex
- EDIT — Review causal chemistry and add sourced molecular context
  Reviewed the entire record against MetaCyc 22.5 native pathway XML (including nested pathways), Rhea 139 explicit sides and directions, ENZYME 02-Sep-2026, PubMed records, and reviewed UniProt 2026_03 annotations within the record's taxon scope. Replaced generated structured-source quotations with source_assertion and source_locator. Typed molecular activities and participants. Reaction count 7 to 7; 0 directed-ID corrections; 10 protein annotations, 7 cofactor annotations and 7 sourced locations. All Rhea reaction substrate/product sets checked against independent Rhea authority; native pathway leaf coverage and source exceptions recorded in reports/causal_graph_review/metacyc-review.json. Full source URLs, versions and byte hashes are in metacyc-sources.json. All 50 MetaCyc records passed native schema validation; six targeted chemistry/evidence regression tests passed. Missing protein or cofactor annotations are not treated as biological absence; qualifications and exclusions are preserved in the ledger.
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