methylerythritol phosphate pathway I

MetaCyc:NONMEVIPP-PWY

The methylerythritol phosphate (MEP/DOXP) route converts pyruvate and D-glyceraldehyde 3-phosphate to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). This record represents the Escherichia coli K-12 enzyme set; the native MetaCyc pathway also lists K-12 MG1655 and Brucella abortus. IspH directly supplies both products; Idi is an additional interconversion, not an obligatory route to DMAPP. Variant I uses flavodoxin for IspG. Current Rhea IspH equations use generic ferredoxin, whereas primary E. coli assays support flavodoxin-dependent activation; unresolved carrier-state edges are omitted. This partial chemical graph is not a stoichiometric flux model, and source reaction directions do not imply irreversibility.

Pathway type
isoprenoid precursor biosynthesis
Organism scope

Read the source YAML

Pathway network

44 nodes · 68 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Pathway network: methylerythritol phosphate pathway I 44 nodes · 68 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. CHEBI:59776 — consumes → RHEA:12606; Evidence: RHEA:12606consumes CHEBI:15361 — consumes → RHEA:12606; Evidence: RHEA:12606consumes CHEBI:15378 — consumes → RHEA:12606; Evidence: RHEA:12606consumes RHEA:12606 — produces → CHEBI:57792; Evidence: RHEA:12606produces RHEA:12606 — produces → CHEBI:16526; Evidence: RHEA:12606produces UniProtKB:P77488 — catalyzes → RHEA:12606; Evidence: UniProtKB:P77488catalyzes CHEBI:57792 — consumes → RHEA:13719; Evidence: RHEA:13719consumes CHEBI:57783 — consumes → RHEA:13719; Evidence: RHEA:13719consumes CHEBI:15378 — consumes → RHEA:13719; Evidence: RHEA:13719consumes RHEA:13719 — produces → CHEBI:58262; Evidence: RHEA:13719produces RHEA:13719 — produces → CHEBI:58349; Evidence: RHEA:13719produces UniProtKB:P45568 — catalyzes → RHEA:13719; Evidence: UniProtKB:P45568catalyzes CHEBI:58262 — consumes → RHEA:13430; Evidence: RHEA:13430consumes CHEBI:37563 — consumes → RHEA:13430; Evidence: RHEA:13430consumes CHEBI:15378 — consumes → RHEA:13430; Evidence: RHEA:13430consumes RHEA:13430 — produces → CHEBI:57823; Evidence: RHEA:13430produces RHEA:13430 — produces → CHEBI:33019; Evidence: RHEA:13430produces UniProtKB:Q46893 — catalyzes → RHEA:13430; Evidence: UniProtKB:Q46893catalyzes CHEBI:57823 — consumes → RHEA:18438; Evidence: RHEA:18438consumes CHEBI:30616 — consumes → RHEA:18438; Evidence: RHEA:18438consumes RHEA:18438 — produces → CHEBI:57919; Evidence: RHEA:18438produces RHEA:18438 — produces → CHEBI:456216; Evidence: RHEA:18438produces RHEA:18438 — produces → CHEBI:15378; Evidence: RHEA:18438produces UniProtKB:P62615 — catalyzes → RHEA:18438; Evidence: UniProtKB:P62615catalyzes CHEBI:57919 — consumes → RHEA:23865; Evidence: RHEA:23865consumes RHEA:23865 — produces → CHEBI:58483; Evidence: RHEA:23865produces RHEA:23865 — produces → CHEBI:60377; Evidence: RHEA:23865produces UniProtKB:P62617 — catalyzes → RHEA:23865; Evidence: UniProtKB:P62617catalyzes CHEBI:58483 — consumes → RHEA:43606; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:43606consumes RHEA:43606 — produces → CHEBI:128753; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:43606produces RHEA:43606 — produces → CHEBI:15377; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:43606produces RHEA:43606 — produces → CHEBI:15378; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:43606produces UniProtKB:P62620 — catalyzes → RHEA:43606; Evidence: UniProtKB:P62620catalyzes CHEBI:128753 — consumes → RHEA:24490; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:24490consumes CHEBI:15378 — consumes → RHEA:24490; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:24490consumes RHEA:24490 — produces → CHEBI:128769; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:24490produces RHEA:24490 — produces → CHEBI:15377; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:24490produces UniProtKB:P62623 — catalyzes → RHEA:24490; The supported IspH chemical conversion is retained. Its UniProt/Rhea generic ferredoxin equation does not establish the E. coli physiological donor; flavodoxin-dependent activation was observed in PMID:12571359.; Evidence: UniProtKB:P62623catalyzes CHEBI:128753 — consumes → RHEA:24827; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:24827consumes CHEBI:15378 — consumes → RHEA:24827; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:24827consumes RHEA:24827 — produces → CHEBI:57623; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:24827produces RHEA:24827 — produces → CHEBI:15377; Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.; Evidence: RHEA:24827produces UniProtKB:P62623 — catalyzes → RHEA:24827; The supported IspH chemical conversion is retained. Its UniProt/Rhea generic ferredoxin equation does not establish the E. coli physiological donor; flavodoxin-dependent activation was observed in PMID:12571359.; Evidence: UniProtKB:P62623catalyzes CHEBI:128769 — consumes → RHEA:23285; Evidence: RHEA:23285consumes RHEA:23285 — produces → CHEBI:57623; Evidence: RHEA:23285produces UniProtKB:Q46822 — catalyzes → RHEA:23285; Additional IPP/DMAPP interconversion. IspH already produces both products; this is not an obligatory DMAPP-forming step. Left-to-right is the source-selected orientation, not a claim of irreversibility.; Evidence: UniProtKB:Q46822catalyzes RHEA:43606 — precedes → RHEA:24490; Native pathway ordering projected to the reviewed reaction mappings.; Evidence: MetaCyc:NONMEVIPP-PWYprecedes RHEA:43606 — precedes → RHEA:24827; Native pathway ordering projected to the reviewed reaction mappings.; Evidence: MetaCyc:NONMEVIPP-PWYprecedes RHEA:23865 — precedes → RHEA:43606; Native pathway ordering projected to the reviewed reaction mappings.; Evidence: MetaCyc:NONMEVIPP-PWYprecedes RHEA:13719 — precedes → RHEA:13430; Native pathway ordering projected to the reviewed reaction mappings.; Evidence: MetaCyc:NONMEVIPP-PWYprecedes RHEA:12606 — precedes → RHEA:13719; Native pathway ordering projected to the reviewed reaction mappings.; Evidence: MetaCyc:NONMEVIPP-PWYprecedes RHEA:13430 — precedes → RHEA:18438; Native pathway ordering projected to the reviewed reaction mappings.; Evidence: MetaCyc:NONMEVIPP-PWYprecedes RHEA:18438 — precedes → RHEA:23865; Native pathway ordering projected to the reviewed reaction mappings.; Evidence: MetaCyc:NONMEVIPP-PWYprecedes RHEA:24490 — precedes → RHEA:23285; Native ordering places optional Idi after the IPP-forming IspH branch; the separate IspH DMAPP-producing branch is retained and does not require Idi.; Evidence: MetaCyc:NONMEVIPP-PWYprecedes UniProtKB:P77488 — has_cofactor → CHEBI:18420; Binds 1 Mg(2+) ion per subunit.; Evidence: UniProtKB:P77488has cofactor UniProtKB:P77488 — has_cofactor → CHEBI:58937; Binds 1 thiamine pyrophosphate per subunit.; Evidence: UniProtKB:P77488has cofactor UniProtKB:P45568 — has_cofactor → CHEBI:18420; Divalent cation. Prefers Mg(2+), Mn(2+) or Co(2+). These listed metal possibilities do not assert that all three metals are simultaneously required.; Evidence: UniProtKB:P45568has cofactor UniProtKB:P45568 — has_cofactor → CHEBI:29035; Divalent cation. Prefers Mg(2+), Mn(2+) or Co(2+). These listed metal possibilities do not assert that all three metals are simultaneously required.; Evidence: UniProtKB:P45568has cofactor UniProtKB:P45568 — has_cofactor → CHEBI:48828; Divalent cation. Prefers Mg(2+), Mn(2+) or Co(2+). These listed metal possibilities do not assert that all three metals are simultaneously required.; Evidence: UniProtKB:P45568has cofactor UniProtKB:Q46893 — has_cofactor → CHEBI:18420; These listed metal possibilities do not assert that all three metals are simultaneously required. The source field supplies no evidence code; no direct experimental claim is inferred.; Evidence: UniProtKB:Q46893has cofactor UniProtKB:Q46893 — has_cofactor → CHEBI:29035; These listed metal possibilities do not assert that all three metals are simultaneously required. The source field supplies no evidence code; no direct experimental claim is inferred.; Evidence: UniProtKB:Q46893has cofactor UniProtKB:Q46893 — has_cofactor → CHEBI:48828; These listed metal possibilities do not assert that all three metals are simultaneously required. The source field supplies no evidence code; no direct experimental claim is inferred.; Evidence: UniProtKB:Q46893has cofactor UniProtKB:P62617 — has_cofactor → CHEBI:60240; Binds 1 divalent metal cation per subunit.; Evidence: UniProtKB:P62617has cofactor UniProtKB:P62620 — has_cofactor → CHEBI:49883; Binds 1 [4Fe-4S] cluster.; Evidence: UniProtKB:P62620has cofactor UniProtKB:P62623 — has_cofactor → CHEBI:49883; Was shown to bind 1 [3Fe-4S] cluster per subunit (PubMed:15469281, PubMed:19569147, PubMed:20080550). However, it initially contains a [4Fe-4S] cluster which easily degrades into a [3Fe-4S] form in the presence of oxygen (PubMed:12706830, PubMed:20080550, PubMed:22137895, PubMed:22687151).; Evidence: UniProtKB:P62623has cofactor UniProtKB:Q46822 — has_cofactor → CHEBI:18420; Binds 1 Mg(2+) ion per subunit. The magnesium ion binds only when substrate is bound.; Evidence: UniProtKB:Q46822has cofactor UniProtKB:Q46822 — has_cofactor → CHEBI:29035; Binds 1 Mn(2+) ion per subunit.; Evidence: UniProtKB:Q46822has cofactor UniProtKB:Q46822 — located_in → GO:0005737; Contextual localization of Idi only; does not establish localization or exclusivity for other pathway proteins.; Evidence: UniProtKB:Q46822located in D-glyceraldehyde 3-phosphate; CHEBI:59776; Declared type: small_molecule small molecule D-glyceraldehyde 3-phosphate pyruvate; CHEBI:15361; Declared type: small_molecule small molecule pyruvate H(+); CHEBI:15378; Declared type: small_molecule small molecule H(+) 1-deoxy-D-xylulose 5-phosphate; CHEBI:57792; Declared type: small_molecule small molecule 1-deoxy-D-xylulose 5-phosphate CO2; CHEBI:16526; Declared type: small_molecule small molecule CO2 1-deoxy-D-xylulose-5-phosphate synthase; UniProtKB:P77488; Declared type: protein protein 1-deoxy-D- xylulose-5-phosphate synthase NADPH; CHEBI:57783; Declared type: small_molecule small molecule NADPH 2-C-methyl-D-erythritol 4-phosphate; CHEBI:58262; Declared type: small_molecule small molecule 2-C-methyl-D-erythritol 4-phosphate NADP(+); CHEBI:58349; Declared type: small_molecule small molecule NADP(+) 1-deoxy-D-xylulose 5-phosphate reductoisomerase; UniProtKB:P45568; Declared type: protein protein 1-deoxy-D-xylulose 5-phosphate reductoisomerase CTP; CHEBI:37563; Declared type: small_molecule small molecule CTP 4-CDP-2-C-methyl-D-erythritol; CHEBI:57823; Declared type: small_molecule small molecule 4-CDP-2-C-methyl-D- erythritol diphosphate; CHEBI:33019; Declared type: small_molecule small molecule diphosphate 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; UniProtKB:Q46893; Declared type: protein protein 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase ATP; CHEBI:30616; Declared type: small_molecule small molecule ATP 4-CDP-2-C-methyl-D-erythritol 2-phosphate; CHEBI:57919; Declared type: small_molecule small molecule 4-CDP-2-C-methyl-D- erythritol 2-phosphate ADP; CHEBI:456216; Declared type: small_molecule small molecule ADP 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; UniProtKB:P62615; Declared type: protein protein 4-diphosphocytidyl-2-C- methyl-D-erythritol kinase 2-C-methyl-D-erythritol 2,4-cyclic diphosphate; CHEBI:58483; Declared type: small_molecule small molecule 2-C-methyl-D-erythritol 2,4-cyclic diphosphate CMP; CHEBI:60377; Declared type: small_molecule small molecule CMP 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; UniProtKB:P62617; Declared type: protein protein 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate; CHEBI:128753; Declared type: small_molecule small molecule (2E)-4-hydroxy-3-methylbut- 2-enyl diphosphate H2O; CHEBI:15377; Declared type: small_molecule small molecule H2O 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin); UniProtKB:P62620; Declared type: protein protein 4-hydroxy-3-methylbut-2-en- 1-yl diphosphate synthase (flavodoxin) isopentenyl diphosphate; CHEBI:128769; Declared type: small_molecule small molecule isopentenyl diphosphate 4-hydroxy-3-methylbut-2-enyl diphosphate reductase; UniProtKB:P62623; Declared type: protein protein 4-hydroxy-3-methylbut-2-enyl diphosphate reductase dimethylallyl diphosphate; CHEBI:57623; Declared type: small_molecule small molecule dimethylallyl diphosphate Isopentenyl-diphosphate Delta-isomerase; UniProtKB:Q46822; Declared type: protein protein Isopentenyl-diphosphate Delta-isomerase Mg(2+); CHEBI:18420; Declared type: cofactor cofactor Mg(2+) thiamine diphosphate; CHEBI:58937; Declared type: cofactor cofactor thiamine diphosphate Mn(2+); CHEBI:29035; Declared type: cofactor cofactor Mn(2+) Co(2+); CHEBI:48828; Declared type: cofactor cofactor Co(2+) a divalent metal cation; CHEBI:60240; Declared type: cofactor cofactor a divalent metal cation [4Fe-4S] cluster; CHEBI:49883; Declared type: cofactor cofactor [4Fe-4S] cluster cytoplasm; GO:0005737; Declared type: cellular_component cellular component cytoplasm Dxs condensation to 1-deoxy-D-xylulose 5-phosphate; RHEA:12606; Declared type: reaction/activity; Source reaction direction: left_to_right reaction / activity Dxs condensation to 1-deoxy- D-xylulose 5-phosphate Dxr rearrangement and reduction to MEP; RHEA:13719; Declared type: reaction/activity; Source reaction direction: right_to_left reaction / activity Dxr rearrangement and reduction to MEP IspD cytidylylation of MEP; RHEA:13430; Declared type: reaction/activity; Source reaction direction: left_to_right reaction / activity IspD cytidylylation of MEP IspE phosphorylation of CDP-ME; RHEA:18438; Declared type: reaction/activity; Source reaction direction: left_to_right reaction / activity IspE phosphorylation of CDP- ME IspF formation of methylerythritol cyclic diphosphate; RHEA:23865; Declared type: reaction/activity; Source reaction direction: left_to_right reaction / activity IspF formation of methylerythritol cyclic diphosphate IspG reduction to HMBPP (flavodoxin reaction family); RHEA:43606; Declared type: reaction/activity; Source reaction direction: right_to_left reaction / activity IspG reduction to HMBPP (flavodoxin reaction family) IspH HMBPP reduction to IPP (chemical-side projection); RHEA:24490; Declared type: reaction/activity; Source reaction direction: right_to_left reaction / activity IspH HMBPP reduction to IPP (chemical-side projection) IspH HMBPP reduction to DMAPP (chemical-side projection); RHEA:24827; Declared type: reaction/activity; Source reaction direction: right_to_left reaction / activity IspH HMBPP reduction to DMAPP (chemical-side projection) Idi interconversion of IPP to DMAPP; RHEA:23285; Declared type: reaction/activity; Source reaction direction: left_to_right reaction / activity Idi interconversion of IPP to DMAPP

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Escherichia coli K-12
NCBITaxon:83333		taxon
D-glyceraldehyde 3-phosphate
CHEBI:59776		small molecule
pyruvate
CHEBI:15361		small molecule
H(+)
CHEBI:15378		small molecule
1-deoxy-D-xylulose 5-phosphate
CHEBI:57792		small molecule
CO2
CHEBI:16526		small molecule
1-deoxy-D-xylulose-5-phosphate synthase
UniProtKB:P77488		protein
NADPH
CHEBI:57783		small molecule
2-C-methyl-D-erythritol 4-phosphate
CHEBI:58262		small molecule
NADP(+)
CHEBI:58349		small molecule
1-deoxy-D-xylulose 5-phosphate reductoisomerase
UniProtKB:P45568		protein
CTP
CHEBI:37563		small molecule
4-CDP-2-C-methyl-D-erythritol
CHEBI:57823		small molecule
diphosphate
CHEBI:33019		small molecule
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
UniProtKB:Q46893		protein
ATP
CHEBI:30616		small molecule
4-CDP-2-C-methyl-D-erythritol 2-phosphate
CHEBI:57919		small molecule
ADP
CHEBI:456216		small molecule
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
UniProtKB:P62615		protein
2-C-methyl-D-erythritol 2,4-cyclic diphosphate
CHEBI:58483		small molecule
CMP
CHEBI:60377		small molecule
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
UniProtKB:P62617		protein
(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate
CHEBI:128753		small molecule
H2O
CHEBI:15377		small molecule
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
UniProtKB:P62620		protein
isopentenyl diphosphate
CHEBI:128769		small molecule
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
UniProtKB:P62623		protein
dimethylallyl diphosphate
CHEBI:57623		small molecule
Isopentenyl-diphosphate Delta-isomerase
UniProtKB:Q46822		protein
Mg(2+)
CHEBI:18420		cofactor
thiamine diphosphate
CHEBI:58937		cofactor
Mn(2+)
CHEBI:29035		cofactor
Co(2+)
CHEBI:48828		cofactor
a divalent metal cation
CHEBI:60240		cofactor
[4Fe-4S] cluster
CHEBI:49883		cofactor
cytoplasm
GO:0005737		cellular component
Dxs condensation to 1-deoxy-D-xylulose 5-phosphate
RHEA:12606		reactionleft to right
Dxr rearrangement and reduction to MEP
RHEA:13719		reactionright to left
IspD cytidylylation of MEP
RHEA:13430		reactionleft to right
IspE phosphorylation of CDP-ME
RHEA:18438		reactionleft to right
IspF formation of methylerythritol cyclic diphosphate
RHEA:23865		reactionleft to right
IspG reduction to HMBPP (flavodoxin reaction family)
RHEA:43606		reactionright to left
IspH HMBPP reduction to IPP (chemical-side projection)
RHEA:24490		reactionright to left
IspH HMBPP reduction to DMAPP (chemical-side projection)
RHEA:24827		reactionright to left
Idi interconversion of IPP to DMAPP
RHEA:23285		reactionleft to right

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
D-glyceraldehyde 3-phosphate
CHEBI:59776		consumes

Dxs condensation to 1-deoxy-D-xylulose 5-phosphate
RHEA:12606
  • RHEA:12606

    Source assertion: Rhea RHEA:12606 places CHEBI:59776 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/12606']/rh:substrates; http://rdf.rhea-db.org/12605_L/rh:contains1; http://rdf.rhea-db.org/Participant_12605_compound_6062/rh:compound; http://rdf.rhea-db.org/Compound_6062/rh:accession
pyruvate
CHEBI:15361		consumes

Dxs condensation to 1-deoxy-D-xylulose 5-phosphate
RHEA:12606
  • RHEA:12606

    Source assertion: Rhea RHEA:12606 places CHEBI:15361 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/12606']/rh:substrates; http://rdf.rhea-db.org/12605_L/rh:contains1; http://rdf.rhea-db.org/Participant_12605_compound_3719/rh:compound; http://rdf.rhea-db.org/Compound_3719/rh:accession
H(+)
CHEBI:15378		consumes

Dxs condensation to 1-deoxy-D-xylulose 5-phosphate
RHEA:12606
  • RHEA:12606

    Source assertion: Rhea RHEA:12606 places CHEBI:15378 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/12606']/rh:substrates; http://rdf.rhea-db.org/12605_L/rh:contains1; http://rdf.rhea-db.org/Participant_12605_compound_3249/rh:compound; http://rdf.rhea-db.org/Compound_3249/rh:accession
Dxs condensation to 1-deoxy-D-xylulose 5-phosphate
RHEA:12606		produces

1-deoxy-D-xylulose 5-phosphate
CHEBI:57792
  • RHEA:12606

    Source assertion: Rhea RHEA:12606 places CHEBI:57792 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/12606']/rh:products; http://rdf.rhea-db.org/12605_R/rh:contains1; http://rdf.rhea-db.org/Participant_12605_compound_2654/rh:compound; http://rdf.rhea-db.org/Compound_2654/rh:accession
Dxs condensation to 1-deoxy-D-xylulose 5-phosphate
RHEA:12606		produces

CO2
CHEBI:16526
  • RHEA:12606

    Source assertion: Rhea RHEA:12606 places CHEBI:16526 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/12606']/rh:products; http://rdf.rhea-db.org/12605_R/rh:contains1; http://rdf.rhea-db.org/Participant_12605_compound_4391/rh:compound; http://rdf.rhea-db.org/Compound_4391/rh:accession
1-deoxy-D-xylulose-5-phosphate synthase
UniProtKB:P77488		catalyzes

Dxs condensation to 1-deoxy-D-xylulose 5-phosphate
RHEA:12606
  • UniProtKB:P77488

    Source assertion: UniProt UniProtKB:P77488 assigns catalytic activity to RHEA:12605, the reaction family of RHEA:12606; organism NCBITaxon:83333. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P77488'].comments[1].reaction
1-deoxy-D-xylulose 5-phosphate
CHEBI:57792		consumes

Dxr rearrangement and reduction to MEP
RHEA:13719
  • RHEA:13719

    Source assertion: Rhea RHEA:13719 places CHEBI:57792 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13719']/rh:substrates; http://rdf.rhea-db.org/13717_R/rh:contains1; http://rdf.rhea-db.org/Participant_13717_compound_2654/rh:compound; http://rdf.rhea-db.org/Compound_2654/rh:accession
NADPH
CHEBI:57783		consumes

Dxr rearrangement and reduction to MEP
RHEA:13719
  • RHEA:13719

    Source assertion: Rhea RHEA:13719 places CHEBI:57783 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13719']/rh:substrates; http://rdf.rhea-db.org/13717_R/rh:contains1; http://rdf.rhea-db.org/Participant_13717_compound_3438/rh:compound; http://rdf.rhea-db.org/Compound_3438/rh:accession
H(+)
CHEBI:15378		consumes

Dxr rearrangement and reduction to MEP
RHEA:13719
  • RHEA:13719

    Source assertion: Rhea RHEA:13719 places CHEBI:15378 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13719']/rh:substrates; http://rdf.rhea-db.org/13717_R/rh:contains1; http://rdf.rhea-db.org/Participant_13717_compound_3249/rh:compound; http://rdf.rhea-db.org/Compound_3249/rh:accession
Dxr rearrangement and reduction to MEP
RHEA:13719		produces

2-C-methyl-D-erythritol 4-phosphate
CHEBI:58262
  • RHEA:13719

    Source assertion: Rhea RHEA:13719 places CHEBI:58262 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13719']/rh:products; http://rdf.rhea-db.org/13717_L/rh:contains1; http://rdf.rhea-db.org/Participant_13717_compound_3223/rh:compound; http://rdf.rhea-db.org/Compound_3223/rh:accession
Dxr rearrangement and reduction to MEP
RHEA:13719		produces

NADP(+)
CHEBI:58349
  • RHEA:13719

    Source assertion: Rhea RHEA:13719 places CHEBI:58349 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13719']/rh:products; http://rdf.rhea-db.org/13717_L/rh:contains1; http://rdf.rhea-db.org/Participant_13717_compound_5188/rh:compound; http://rdf.rhea-db.org/Compound_5188/rh:accession
1-deoxy-D-xylulose 5-phosphate reductoisomerase
UniProtKB:P45568		catalyzes

Dxr rearrangement and reduction to MEP
RHEA:13719
  • UniProtKB:P45568

    Source assertion: UniProt UniProtKB:P45568 assigns catalytic activity to RHEA:13717, the reaction family of RHEA:13719; organism NCBITaxon:83333. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P45568'].comments[1].reaction
2-C-methyl-D-erythritol 4-phosphate
CHEBI:58262		consumes

IspD cytidylylation of MEP
RHEA:13430
  • RHEA:13430

    Source assertion: Rhea RHEA:13430 places CHEBI:58262 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13430']/rh:substrates; http://rdf.rhea-db.org/13429_L/rh:contains1; http://rdf.rhea-db.org/Participant_13429_compound_3223/rh:compound; http://rdf.rhea-db.org/Compound_3223/rh:accession
CTP
CHEBI:37563		consumes

IspD cytidylylation of MEP
RHEA:13430
  • RHEA:13430

    Source assertion: Rhea RHEA:13430 places CHEBI:37563 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13430']/rh:substrates; http://rdf.rhea-db.org/13429_L/rh:contains1; http://rdf.rhea-db.org/Participant_13429_compound_6434/rh:compound; http://rdf.rhea-db.org/Compound_6434/rh:accession
H(+)
CHEBI:15378		consumes

IspD cytidylylation of MEP
RHEA:13430
  • RHEA:13430

    Source assertion: Rhea RHEA:13430 places CHEBI:15378 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13430']/rh:substrates; http://rdf.rhea-db.org/13429_L/rh:contains1; http://rdf.rhea-db.org/Participant_13429_compound_3249/rh:compound; http://rdf.rhea-db.org/Compound_3249/rh:accession
IspD cytidylylation of MEP
RHEA:13430		produces

4-CDP-2-C-methyl-D-erythritol
CHEBI:57823
  • RHEA:13430

    Source assertion: Rhea RHEA:13430 places CHEBI:57823 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13430']/rh:products; http://rdf.rhea-db.org/13429_R/rh:contains1; http://rdf.rhea-db.org/Participant_13429_compound_1305/rh:compound; http://rdf.rhea-db.org/Compound_1305/rh:accession
IspD cytidylylation of MEP
RHEA:13430		produces

diphosphate
CHEBI:33019
  • RHEA:13430

    Source assertion: Rhea RHEA:13430 places CHEBI:33019 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/13430']/rh:products; http://rdf.rhea-db.org/13429_R/rh:contains1; http://rdf.rhea-db.org/Participant_13429_compound_2106/rh:compound; http://rdf.rhea-db.org/Compound_2106/rh:accession
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
UniProtKB:Q46893		catalyzes

IspD cytidylylation of MEP
RHEA:13430
  • UniProtKB:Q46893

    Source assertion: UniProt UniProtKB:Q46893 assigns catalytic activity to RHEA:13429, the reaction family of RHEA:13430; organism NCBITaxon:83333. Annotation evidence: no evidence code supplied on this source field.

    Source locationresults[primaryAccession='Q46893'].comments[1].reaction
4-CDP-2-C-methyl-D-erythritol
CHEBI:57823		consumes

IspE phosphorylation of CDP-ME
RHEA:18438
  • RHEA:18438

    Source assertion: Rhea RHEA:18438 places CHEBI:57823 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/18438']/rh:substrates; http://rdf.rhea-db.org/18437_L/rh:contains1; http://rdf.rhea-db.org/Participant_18437_compound_1305/rh:compound; http://rdf.rhea-db.org/Compound_1305/rh:accession
ATP
CHEBI:30616		consumes

IspE phosphorylation of CDP-ME
RHEA:18438
  • RHEA:18438

    Source assertion: Rhea RHEA:18438 places CHEBI:30616 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/18438']/rh:substrates; http://rdf.rhea-db.org/18437_L/rh:contains1; http://rdf.rhea-db.org/Participant_18437_compound_6372/rh:compound; http://rdf.rhea-db.org/Compound_6372/rh:accession
IspE phosphorylation of CDP-ME
RHEA:18438		produces

4-CDP-2-C-methyl-D-erythritol 2-phosphate
CHEBI:57919
  • RHEA:18438

    Source assertion: Rhea RHEA:18438 places CHEBI:57919 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/18438']/rh:products; http://rdf.rhea-db.org/18437_R/rh:contains1; http://rdf.rhea-db.org/Participant_18437_compound_1085/rh:compound; http://rdf.rhea-db.org/Compound_1085/rh:accession
IspE phosphorylation of CDP-ME
RHEA:18438		produces

ADP
CHEBI:456216
  • RHEA:18438

    Source assertion: Rhea RHEA:18438 places CHEBI:456216 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/18438']/rh:products; http://rdf.rhea-db.org/18437_R/rh:contains1; http://rdf.rhea-db.org/Participant_18437_compound_2710/rh:compound; http://rdf.rhea-db.org/Compound_2710/rh:accession
IspE phosphorylation of CDP-ME
RHEA:18438		produces

H(+)
CHEBI:15378
  • RHEA:18438

    Source assertion: Rhea RHEA:18438 places CHEBI:15378 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/18438']/rh:products; http://rdf.rhea-db.org/18437_R/rh:contains1; http://rdf.rhea-db.org/Participant_18437_compound_3249/rh:compound; http://rdf.rhea-db.org/Compound_3249/rh:accession
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
UniProtKB:P62615		catalyzes

IspE phosphorylation of CDP-ME
RHEA:18438
  • UniProtKB:P62615

    Source assertion: UniProt UniProtKB:P62615 assigns catalytic activity to RHEA:18437, the reaction family of RHEA:18438; organism NCBITaxon:83333. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P62615'].comments[1].reaction
4-CDP-2-C-methyl-D-erythritol 2-phosphate
CHEBI:57919		consumes

IspF formation of methylerythritol cyclic diphosphate
RHEA:23865
  • RHEA:23865

    Source assertion: Rhea RHEA:23865 places CHEBI:57919 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/23865']/rh:substrates; http://rdf.rhea-db.org/23864_L/rh:contains1; http://rdf.rhea-db.org/Participant_23864_compound_1085/rh:compound; http://rdf.rhea-db.org/Compound_1085/rh:accession
IspF formation of methylerythritol cyclic diphosphate
RHEA:23865		produces

2-C-methyl-D-erythritol 2,4-cyclic diphosphate
CHEBI:58483
  • RHEA:23865

    Source assertion: Rhea RHEA:23865 places CHEBI:58483 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/23865']/rh:products; http://rdf.rhea-db.org/23864_R/rh:contains1; http://rdf.rhea-db.org/Participant_23864_compound_2953/rh:compound; http://rdf.rhea-db.org/Compound_2953/rh:accession
IspF formation of methylerythritol cyclic diphosphate
RHEA:23865		produces

CMP
CHEBI:60377
  • RHEA:23865

    Source assertion: Rhea RHEA:23865 places CHEBI:60377 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/23865']/rh:products; http://rdf.rhea-db.org/23864_R/rh:contains1; http://rdf.rhea-db.org/Participant_23864_compound_3543/rh:compound; http://rdf.rhea-db.org/Compound_3543/rh:accession
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
UniProtKB:P62617		catalyzes

IspF formation of methylerythritol cyclic diphosphate
RHEA:23865
  • UniProtKB:P62617

    Source assertion: UniProt UniProtKB:P62617 assigns catalytic activity to RHEA:23864, the reaction family of RHEA:23865; organism NCBITaxon:83333. Annotation evidence: ECO:0000255, ECO:0000269.

    Source locationresults[primaryAccession='P62617'].comments[1].reaction
2-C-methyl-D-erythritol 2,4-cyclic diphosphate
CHEBI:58483		consumes

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

IspG reduction to HMBPP (flavodoxin reaction family)
RHEA:43606
  • RHEA:43606

    Source assertion: Rhea RHEA:43606 places CHEBI:58483 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/43606']/rh:substrates; http://rdf.rhea-db.org/43604_R/rh:contains1; http://rdf.rhea-db.org/Participant_43604_compound_2953/rh:compound; http://rdf.rhea-db.org/Compound_2953/rh:accession
IspG reduction to HMBPP (flavodoxin reaction family)
RHEA:43606		produces

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate
CHEBI:128753
  • RHEA:43606

    Source assertion: Rhea RHEA:43606 places CHEBI:128753 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/43606']/rh:products; http://rdf.rhea-db.org/43604_L/rh:contains1; http://rdf.rhea-db.org/Participant_43604_compound_2971/rh:compound; http://rdf.rhea-db.org/Compound_2971/rh:accession
IspG reduction to HMBPP (flavodoxin reaction family)
RHEA:43606		produces

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

H2O
CHEBI:15377
  • RHEA:43606

    Source assertion: Rhea RHEA:43606 places CHEBI:15377 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/43606']/rh:products; http://rdf.rhea-db.org/43604_L/rh:contains1; http://rdf.rhea-db.org/Participant_43604_compound_1283/rh:compound; http://rdf.rhea-db.org/Compound_1283/rh:accession
IspG reduction to HMBPP (flavodoxin reaction family)
RHEA:43606		produces

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

H(+)
CHEBI:15378
  • RHEA:43606

    Source assertion: Rhea RHEA:43606 places CHEBI:15378 on its products side; coefficient 2.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/43606']/rh:products; http://rdf.rhea-db.org/43604_L/rh:contains2; http://rdf.rhea-db.org/Participant_43604_compound_3249/rh:compound; http://rdf.rhea-db.org/Compound_3249/rh:accession
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
UniProtKB:P62620		catalyzes

IspG reduction to HMBPP (flavodoxin reaction family)
RHEA:43606
  • UniProtKB:P62620

    Source assertion: UniProt UniProtKB:P62620 assigns catalytic activity to RHEA:43604, the reaction family of RHEA:43606; organism NCBITaxon:83333. Annotation evidence: ECO:0000255, ECO:0000269.

    Source locationresults[primaryAccession='P62620'].comments[1].reaction
(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate
CHEBI:128753		consumes

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

IspH HMBPP reduction to IPP (chemical-side projection)
RHEA:24490
  • RHEA:24490

    Source assertion: Rhea RHEA:24490 places CHEBI:128753 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/24490']/rh:substrates; http://rdf.rhea-db.org/24488_R/rh:contains1; http://rdf.rhea-db.org/Participant_24488_compound_2971/rh:compound; http://rdf.rhea-db.org/Compound_2971/rh:accession
H(+)
CHEBI:15378		consumes

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

IspH HMBPP reduction to IPP (chemical-side projection)
RHEA:24490
  • RHEA:24490

    Source assertion: Rhea RHEA:24490 places CHEBI:15378 on its substrates side; coefficient 2.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/24490']/rh:substrates; http://rdf.rhea-db.org/24488_R/rh:contains2; http://rdf.rhea-db.org/Participant_24488_compound_3249/rh:compound; http://rdf.rhea-db.org/Compound_3249/rh:accession
IspH HMBPP reduction to IPP (chemical-side projection)
RHEA:24490		produces

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

isopentenyl diphosphate
CHEBI:128769
  • RHEA:24490

    Source assertion: Rhea RHEA:24490 places CHEBI:128769 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/24490']/rh:products; http://rdf.rhea-db.org/24488_L/rh:contains1; http://rdf.rhea-db.org/Participant_24488_compound_4425/rh:compound; http://rdf.rhea-db.org/Compound_4425/rh:accession
IspH HMBPP reduction to IPP (chemical-side projection)
RHEA:24490		produces

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

H2O
CHEBI:15377
  • RHEA:24490

    Source assertion: Rhea RHEA:24490 places CHEBI:15377 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/24490']/rh:products; http://rdf.rhea-db.org/24488_L/rh:contains1; http://rdf.rhea-db.org/Participant_24488_compound_1283/rh:compound; http://rdf.rhea-db.org/Compound_1283/rh:accession
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
UniProtKB:P62623		catalyzes

The supported IspH chemical conversion is retained. Its UniProt/Rhea generic ferredoxin equation does not establish the E. coli physiological donor; flavodoxin-dependent activation was observed in PMID:12571359.

IspH HMBPP reduction to IPP (chemical-side projection)
RHEA:24490
  • UniProtKB:P62623

    Source assertion: UniProt UniProtKB:P62623 assigns catalytic activity to RHEA:24488, the reaction family of RHEA:24490; organism NCBITaxon:83333. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P62623'].comments[1].reaction
(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate
CHEBI:128753		consumes

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

IspH HMBPP reduction to DMAPP (chemical-side projection)
RHEA:24827
  • RHEA:24827

    Source assertion: Rhea RHEA:24827 places CHEBI:128753 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/24827']/rh:substrates; http://rdf.rhea-db.org/24825_R/rh:contains1; http://rdf.rhea-db.org/Participant_24825_compound_2971/rh:compound; http://rdf.rhea-db.org/Compound_2971/rh:accession
H(+)
CHEBI:15378		consumes

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

IspH HMBPP reduction to DMAPP (chemical-side projection)
RHEA:24827
  • RHEA:24827

    Source assertion: Rhea RHEA:24827 places CHEBI:15378 on its substrates side; coefficient 2.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/24827']/rh:substrates; http://rdf.rhea-db.org/24825_R/rh:contains2; http://rdf.rhea-db.org/Participant_24825_compound_3249/rh:compound; http://rdf.rhea-db.org/Compound_3249/rh:accession
IspH HMBPP reduction to DMAPP (chemical-side projection)
RHEA:24827		produces

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

dimethylallyl diphosphate
CHEBI:57623
  • RHEA:24827

    Source assertion: Rhea RHEA:24827 places CHEBI:57623 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/24827']/rh:products; http://rdf.rhea-db.org/24825_L/rh:contains1; http://rdf.rhea-db.org/Participant_24825_compound_1833/rh:compound; http://rdf.rhea-db.org/Compound_1833/rh:accession
IspH HMBPP reduction to DMAPP (chemical-side projection)
RHEA:24827		produces

Chemical-side projection only: source generic electron-carrier states are not represented. This edge does not establish a balanced complete reaction or the physiological donor identity.

H2O
CHEBI:15377
  • RHEA:24827

    Source assertion: Rhea RHEA:24827 places CHEBI:15377 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/24827']/rh:products; http://rdf.rhea-db.org/24825_L/rh:contains1; http://rdf.rhea-db.org/Participant_24825_compound_1283/rh:compound; http://rdf.rhea-db.org/Compound_1283/rh:accession
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
UniProtKB:P62623		catalyzes

The supported IspH chemical conversion is retained. Its UniProt/Rhea generic ferredoxin equation does not establish the E. coli physiological donor; flavodoxin-dependent activation was observed in PMID:12571359.

IspH HMBPP reduction to DMAPP (chemical-side projection)
RHEA:24827
  • UniProtKB:P62623

    Source assertion: UniProt UniProtKB:P62623 assigns catalytic activity to RHEA:24825, the reaction family of RHEA:24827; organism NCBITaxon:83333. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P62623'].comments[2].reaction
isopentenyl diphosphate
CHEBI:128769		consumes

Idi interconversion of IPP to DMAPP
RHEA:23285
  • RHEA:23285

    Source assertion: Rhea RHEA:23285 places CHEBI:128769 on its substrates side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/23285']/rh:substrates; http://rdf.rhea-db.org/23284_L/rh:contains1; http://rdf.rhea-db.org/Participant_23284_compound_4425/rh:compound; http://rdf.rhea-db.org/Compound_4425/rh:accession
Idi interconversion of IPP to DMAPP
RHEA:23285		produces

dimethylallyl diphosphate
CHEBI:57623
  • RHEA:23285

    Source assertion: Rhea RHEA:23285 places CHEBI:57623 on its products side; coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/23285']/rh:products; http://rdf.rhea-db.org/23284_R/rh:contains1; http://rdf.rhea-db.org/Participant_23284_compound_1833/rh:compound; http://rdf.rhea-db.org/Compound_1833/rh:accession
Isopentenyl-diphosphate Delta-isomerase
UniProtKB:Q46822		catalyzes

Additional IPP/DMAPP interconversion. IspH already produces both products; this is not an obligatory DMAPP-forming step. Left-to-right is the source-selected orientation, not a claim of irreversibility.

Idi interconversion of IPP to DMAPP
RHEA:23285
  • UniProtKB:Q46822

    Source assertion: UniProt UniProtKB:Q46822 assigns catalytic activity to RHEA:23284, the reaction family of RHEA:23285; organism NCBITaxon:83333. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='Q46822'].comments[1].reaction
IspG reduction to HMBPP (flavodoxin reaction family)
RHEA:43606		precedes

Native pathway ordering projected to the reviewed reaction mappings.

IspH HMBPP reduction to IPP (chemical-side projection)
RHEA:24490
  • MetaCyc:NONMEVIPP-PWY

    Source assertion: MetaCyc NONMEVIPP-PWY lists RXN-15878 as a predecessor reaction of ISPH2-RXN. The reviewed reaction mappings are RHEA:43606 and RHEA:24490, respectively.

    Source locationPathway[@frameid='NONMEVIPP-PWY']/reaction-ordering[Reaction/@frameid='ISPH2-RXN']/predecessor-reactions/Reaction[@frameid='RXN-15878']
IspG reduction to HMBPP (flavodoxin reaction family)
RHEA:43606		precedes

Native pathway ordering projected to the reviewed reaction mappings.

IspH HMBPP reduction to DMAPP (chemical-side projection)
RHEA:24827
  • MetaCyc:NONMEVIPP-PWY

    Source assertion: MetaCyc NONMEVIPP-PWY lists RXN-15878 as a predecessor reaction of RXN0-884. The reviewed reaction mappings are RHEA:43606 and RHEA:24827, respectively.

    Source locationPathway[@frameid='NONMEVIPP-PWY']/reaction-ordering[Reaction/@frameid='RXN0-884']/predecessor-reactions/Reaction[@frameid='RXN-15878']
IspF formation of methylerythritol cyclic diphosphate
RHEA:23865		precedes

Native pathway ordering projected to the reviewed reaction mappings.

IspG reduction to HMBPP (flavodoxin reaction family)
RHEA:43606
  • MetaCyc:NONMEVIPP-PWY

    Source assertion: MetaCyc NONMEVIPP-PWY lists RXN0-302 as a predecessor reaction of RXN-15878. The reviewed reaction mappings are RHEA:23865 and RHEA:43606, respectively.

    Source locationPathway[@frameid='NONMEVIPP-PWY']/reaction-ordering[Reaction/@frameid='RXN-15878']/predecessor-reactions/Reaction[@frameid='RXN0-302']
Dxr rearrangement and reduction to MEP
RHEA:13719		precedes

Native pathway ordering projected to the reviewed reaction mappings.

IspD cytidylylation of MEP
RHEA:13430
  • MetaCyc:NONMEVIPP-PWY

    Source assertion: MetaCyc NONMEVIPP-PWY lists DXPREDISOM-RXN as a predecessor reaction of 2.7.7.60-RXN. The reviewed reaction mappings are RHEA:13719 and RHEA:13430, respectively.

    Source locationPathway[@frameid='NONMEVIPP-PWY']/reaction-ordering[Reaction/@frameid='2.7.7.60-RXN']/predecessor-reactions/Reaction[@frameid='DXPREDISOM-RXN']
Dxs condensation to 1-deoxy-D-xylulose 5-phosphate
RHEA:12606		precedes

Native pathway ordering projected to the reviewed reaction mappings.

Dxr rearrangement and reduction to MEP
RHEA:13719
  • MetaCyc:NONMEVIPP-PWY

    Source assertion: MetaCyc NONMEVIPP-PWY lists DXS-RXN as a predecessor reaction of DXPREDISOM-RXN. The reviewed reaction mappings are RHEA:12606 and RHEA:13719, respectively.

    Source locationPathway[@frameid='NONMEVIPP-PWY']/reaction-ordering[Reaction/@frameid='DXPREDISOM-RXN']/predecessor-reactions/Reaction[@frameid='DXS-RXN']
IspD cytidylylation of MEP
RHEA:13430		precedes

Native pathway ordering projected to the reviewed reaction mappings.

IspE phosphorylation of CDP-ME
RHEA:18438
  • MetaCyc:NONMEVIPP-PWY

    Source assertion: MetaCyc NONMEVIPP-PWY lists 2.7.7.60-RXN as a predecessor reaction of 2.7.1.148-RXN. The reviewed reaction mappings are RHEA:13430 and RHEA:18438, respectively.

    Source locationPathway[@frameid='NONMEVIPP-PWY']/reaction-ordering[Reaction/@frameid='2.7.1.148-RXN']/predecessor-reactions/Reaction[@frameid='2.7.7.60-RXN']
IspE phosphorylation of CDP-ME
RHEA:18438		precedes

Native pathway ordering projected to the reviewed reaction mappings.

IspF formation of methylerythritol cyclic diphosphate
RHEA:23865
  • MetaCyc:NONMEVIPP-PWY

    Source assertion: MetaCyc NONMEVIPP-PWY lists 2.7.1.148-RXN as a predecessor reaction of RXN0-302. The reviewed reaction mappings are RHEA:18438 and RHEA:23865, respectively.

    Source locationPathway[@frameid='NONMEVIPP-PWY']/reaction-ordering[Reaction/@frameid='RXN0-302']/predecessor-reactions/Reaction[@frameid='2.7.1.148-RXN']
IspH HMBPP reduction to IPP (chemical-side projection)
RHEA:24490		precedes

Native ordering places optional Idi after the IPP-forming IspH branch; the separate IspH DMAPP-producing branch is retained and does not require Idi.

Idi interconversion of IPP to DMAPP
RHEA:23285
  • MetaCyc:NONMEVIPP-PWY

    Source assertion: MetaCyc NONMEVIPP-PWY lists ISPH2-RXN as a predecessor reaction of IPPISOM-RXN. The reviewed reaction mappings are RHEA:24490 and RHEA:23285, respectively.

    Source locationPathway[@frameid='NONMEVIPP-PWY']/reaction-ordering[Reaction/@frameid='IPPISOM-RXN']/predecessor-reactions/Reaction[@frameid='ISPH2-RXN']
1-deoxy-D-xylulose-5-phosphate synthase
UniProtKB:P77488		has_cofactor

Binds 1 Mg(2+) ion per subunit.

Mg(2+)
CHEBI:18420
  • UniProtKB:P77488

    Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:P77488. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P77488'].comments[2].cofactors[0] and note
1-deoxy-D-xylulose-5-phosphate synthase
UniProtKB:P77488		has_cofactor

Binds 1 thiamine pyrophosphate per subunit.

thiamine diphosphate
CHEBI:58937
  • UniProtKB:P77488

    Source assertion: UniProt lists CHEBI:58937 as a cofactor of UniProtKB:P77488. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P77488'].comments[3].cofactors[0] and note
1-deoxy-D-xylulose 5-phosphate reductoisomerase
UniProtKB:P45568		has_cofactor

Divalent cation. Prefers Mg(2+), Mn(2+) or Co(2+). These listed metal possibilities do not assert that all three metals are simultaneously required.

Mg(2+)
CHEBI:18420
  • UniProtKB:P45568

    Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:P45568. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P45568'].comments[2].cofactors[0] and note
1-deoxy-D-xylulose 5-phosphate reductoisomerase
UniProtKB:P45568		has_cofactor

Divalent cation. Prefers Mg(2+), Mn(2+) or Co(2+). These listed metal possibilities do not assert that all three metals are simultaneously required.

Mn(2+)
CHEBI:29035
  • UniProtKB:P45568

    Source assertion: UniProt lists CHEBI:29035 as a cofactor of UniProtKB:P45568. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P45568'].comments[2].cofactors[1] and note
1-deoxy-D-xylulose 5-phosphate reductoisomerase
UniProtKB:P45568		has_cofactor

Divalent cation. Prefers Mg(2+), Mn(2+) or Co(2+). These listed metal possibilities do not assert that all three metals are simultaneously required.

Co(2+)
CHEBI:48828
  • UniProtKB:P45568

    Source assertion: UniProt lists CHEBI:48828 as a cofactor of UniProtKB:P45568. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P45568'].comments[2].cofactors[2] and note
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
UniProtKB:Q46893		has_cofactor

These listed metal possibilities do not assert that all three metals are simultaneously required. The source field supplies no evidence code; no direct experimental claim is inferred.

Mg(2+)
CHEBI:18420
  • UniProtKB:Q46893

    Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:Q46893. Annotation evidence: no evidence code supplied on this source field.

    Source locationresults[primaryAccession='Q46893'].comments[2].cofactors[0] and note
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
UniProtKB:Q46893		has_cofactor

These listed metal possibilities do not assert that all three metals are simultaneously required. The source field supplies no evidence code; no direct experimental claim is inferred.

Mn(2+)
CHEBI:29035
  • UniProtKB:Q46893

    Source assertion: UniProt lists CHEBI:29035 as a cofactor of UniProtKB:Q46893. Annotation evidence: no evidence code supplied on this source field.

    Source locationresults[primaryAccession='Q46893'].comments[2].cofactors[1] and note
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
UniProtKB:Q46893		has_cofactor

These listed metal possibilities do not assert that all three metals are simultaneously required. The source field supplies no evidence code; no direct experimental claim is inferred.

Co(2+)
CHEBI:48828
  • UniProtKB:Q46893

    Source assertion: UniProt lists CHEBI:48828 as a cofactor of UniProtKB:Q46893. Annotation evidence: no evidence code supplied on this source field.

    Source locationresults[primaryAccession='Q46893'].comments[2].cofactors[2] and note
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
UniProtKB:P62617		has_cofactor

Binds 1 divalent metal cation per subunit.

a divalent metal cation
CHEBI:60240
  • UniProtKB:P62617

    Source assertion: UniProt lists CHEBI:60240 as a cofactor of UniProtKB:P62617. Annotation evidence: ECO:0000255, ECO:0000269.

    Source locationresults[primaryAccession='P62617'].comments[3].cofactors[0] and note
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
UniProtKB:P62620		has_cofactor

Binds 1 [4Fe-4S] cluster.

[4Fe-4S] cluster
CHEBI:49883
  • UniProtKB:P62620

    Source assertion: UniProt lists CHEBI:49883 as a cofactor of UniProtKB:P62620. Annotation evidence: ECO:0000255, ECO:0000269.

    Source locationresults[primaryAccession='P62620'].comments[2].cofactors[0] and note
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
UniProtKB:P62623		has_cofactor

Was shown to bind 1 [3Fe-4S] cluster per subunit (PubMed:15469281, PubMed:19569147, PubMed:20080550). However, it initially contains a [4Fe-4S] cluster which easily degrades into a [3Fe-4S] form in the presence of oxygen (PubMed:12706830, PubMed:20080550, PubMed:22137895, PubMed:22687151).

[4Fe-4S] cluster
CHEBI:49883
  • UniProtKB:P62623

    Source assertion: UniProt lists CHEBI:49883 as a cofactor of UniProtKB:P62623. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='P62623'].comments[3].cofactors[0] and note
Isopentenyl-diphosphate Delta-isomerase
UniProtKB:Q46822		has_cofactor

Binds 1 Mg(2+) ion per subunit. The magnesium ion binds only when substrate is bound.

Mg(2+)
CHEBI:18420
  • UniProtKB:Q46822

    Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:Q46822. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='Q46822'].comments[2].cofactors[0] and note
Isopentenyl-diphosphate Delta-isomerase
UniProtKB:Q46822		has_cofactor

Binds 1 Mn(2+) ion per subunit.

Mn(2+)
CHEBI:29035
  • UniProtKB:Q46822

    Source assertion: UniProt lists CHEBI:29035 as a cofactor of UniProtKB:Q46822. Annotation evidence: ECO:0000269.

    Source locationresults[primaryAccession='Q46822'].comments[3].cofactors[0] and note
Isopentenyl-diphosphate Delta-isomerase
UniProtKB:Q46822		located_in

Contextual localization of Idi only; does not establish localization or exclusivity for other pathway proteins.

cytoplasm
GO:0005737
  • UniProtKB:Q46822

    Source assertion: UniProt lists cytoplasm (SL-0086) and explicitly cross-references GO:0005737 for Q46822. The GO location cross-reference has evidence IEA:UniProtKB-SubCell.

    Source locationresults[primaryAccession='Q46822'].uniProtKBCrossReferences[database='GO'][id='GO:0005737'].properties; comments[7].subcellularLocations

References

Curation history