citrate lyase activation

MetaCyc:P2-PWY

The Escherichia coli K-12 protein core of citrate lyase maturation: CitG forms the triphosphoribosyl-dephospho-CoA precursor from ATP and dephospho-CoA; CitX attaches the prosthetic group to the dedicated apo acyl-carrier protein; CitC acetylates its thiol using acetate and ATP. The endpoint is acetylated carrier, not citrate cleavage. Whole apo, holo and acetylated ACP states retain separate generic Rhea protein identifiers and do not identify a particular protein sequence. CitG/CitX have direct biochemical support; the P77390 CitC assignment combines reviewed catalytic annotation, an explicit EcoCyc direct-assay GO mapping and the primary E. coli ligase observation, whose full paper was not inspected. This record does not claim isolated sequence-specific CitC purification or activity in every K-12 strain. AMP-ACP is an off-pathway side product. The multispecies source's alternative acetyltransferase and deacetylase branches are excluded; no citrate-lyase assembly stoichiometry or bound-metal requirement is inferred from assay conditions.

Pathway type

protein prosthetic group biosynthesis and activation

Organism scope

Escherichia coli K-12 NCBITaxon:83333

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Pathway network

17 nodes · 22 asserted directed edges

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Reaction / activity
Chemical / cofactor
Protein / complex
Pathway
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Escherichia coli K-12 `NCBITaxon:83333`	taxon
3'-dephospho-CoA(2-) `CHEBI:57328`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
2'-(5-triphosphoribosyl)-3'-dephospho-CoA(6-) `CHEBI:61378`	small molecule
adenine `CHEBI:16708`	small molecule
diphosphate(3-) `CHEBI:33019`	small molecule
acetate `CHEBI:30089`	small molecule
adenosine 5'-monophosphate(2-) `CHEBI:456215`	small molecule
apo-[citrate lyase ACP] `RHEA-COMP:10157`	protein
holo-[citrate lyase ACP] `RHEA-COMP:10158`	protein
acetyl-[citrate lyase ACP] `RHEA-COMP:13710`	protein
2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase `UniProtKB:P77231`	protein
Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase `UniProtKB:P0A6G5`	protein
[Citrate [pro-3S]-lyase] ligase `UniProtKB:P77390`	protein
3'-dephospho-CoA + ATP => 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine `RHEA:15118`	molecular activity	left to right
apo-[citrate lyase ACP] + 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA => holo-[citrate lyase ACP] + diphosphate `RHEA:16334`	molecular activity	left to right
holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
3'-dephospho-CoA(2-) `CHEBI:57328`	consumes Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	3'-dephospho-CoA + ATP => 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine `RHEA:15118`	RHEA:15117 Source assertion: RHEA:15118 substrates contain CHEBI:57328 with coefficient 1; the compound is 3'-dephospho-CoA. Source location `http://rdf.rhea-db.org/15118 rh:substrates; http://rdf.rhea-db.org/15117_L rh:contains1 http://rdf.rhea-db.org/Participant_15117_compound_1509; rh:compound/rh:accession`
ATP(4-) `CHEBI:30616`	consumes Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	3'-dephospho-CoA + ATP => 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine `RHEA:15118`	RHEA:15117 Source assertion: RHEA:15118 substrates contain CHEBI:30616 with coefficient 1; the compound is ATP. Source location `http://rdf.rhea-db.org/15118 rh:substrates; http://rdf.rhea-db.org/15117_L rh:contains1 http://rdf.rhea-db.org/Participant_15117_compound_6372; rh:compound/rh:accession`
3'-dephospho-CoA + ATP => 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine `RHEA:15118`	produces Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	2'-(5-triphosphoribosyl)-3'-dephospho-CoA(6-) `CHEBI:61378`	RHEA:15117 Source assertion: RHEA:15118 products contain CHEBI:61378 with coefficient 1; the compound is 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA. Source location `http://rdf.rhea-db.org/15118 rh:products; http://rdf.rhea-db.org/15117_R rh:contains1 http://rdf.rhea-db.org/Participant_15117_compound_6441; rh:compound/rh:accession`
3'-dephospho-CoA + ATP => 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine `RHEA:15118`	produces Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	adenine `CHEBI:16708`	RHEA:15117 Source assertion: RHEA:15118 products contain CHEBI:16708 with coefficient 1; the compound is adenine. Source location `http://rdf.rhea-db.org/15118 rh:products; http://rdf.rhea-db.org/15117_R rh:contains1 http://rdf.rhea-db.org/Participant_15117_compound_4174; rh:compound/rh:accession`
apo-[citrate lyase ACP] `RHEA-COMP:10157`	consumes Coefficient 1 of a whole generic citrate-lyase ACP polypeptide state. Its ChEBI reactive-part residue is not substituted for the complete carrier; no sequence-specific identity is asserted.	apo-[citrate lyase ACP] + 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA => holo-[citrate lyase ACP] + diphosphate `RHEA:16334`	RHEA:16333 Source assertion: RHEA:16334 substrates contain GENERIC:10157 with coefficient 1; the compound is apo-[citrate lyase ACP]. Source location `http://rdf.rhea-db.org/16334 rh:substrates; http://rdf.rhea-db.org/16333_L rh:contains1 http://rdf.rhea-db.org/Participant_16333_compound_10157; rh:compound/rh:accession` UniProtKB:P0A6G5 Source assertion: The catalytic-activity Rhea cross-references include RHEA-COMP:10157, the whole ACP state represented by native Rhea Compound_10157. Source location `comments[1].reaction.reactionCrossReferences[database="Rhea"]`
2'-(5-triphosphoribosyl)-3'-dephospho-CoA(6-) `CHEBI:61378`	consumes Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	apo-[citrate lyase ACP] + 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA => holo-[citrate lyase ACP] + diphosphate `RHEA:16334`	RHEA:16333 Source assertion: RHEA:16334 substrates contain CHEBI:61378 with coefficient 1; the compound is 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA. Source location `http://rdf.rhea-db.org/16334 rh:substrates; http://rdf.rhea-db.org/16333_L rh:contains1 http://rdf.rhea-db.org/Participant_16333_compound_6441; rh:compound/rh:accession`
apo-[citrate lyase ACP] + 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA => holo-[citrate lyase ACP] + diphosphate `RHEA:16334`	produces Coefficient 1 of a whole generic citrate-lyase ACP polypeptide state. Its ChEBI reactive-part residue is not substituted for the complete carrier; no sequence-specific identity is asserted.	holo-[citrate lyase ACP] `RHEA-COMP:10158`	RHEA:16333 Source assertion: RHEA:16334 products contain GENERIC:10158 with coefficient 1; the compound is holo-[citrate lyase ACP]. Source location `http://rdf.rhea-db.org/16334 rh:products; http://rdf.rhea-db.org/16333_R rh:contains1 http://rdf.rhea-db.org/Participant_16333_compound_10158; rh:compound/rh:accession` UniProtKB:P0A6G5 Source assertion: The catalytic-activity Rhea cross-references include RHEA-COMP:10158, the whole ACP state represented by native Rhea Compound_10158. Source location `comments[1].reaction.reactionCrossReferences[database="Rhea"]`
apo-[citrate lyase ACP] + 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA => holo-[citrate lyase ACP] + diphosphate `RHEA:16334`	produces Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	diphosphate(3-) `CHEBI:33019`	RHEA:16333 Source assertion: RHEA:16334 products contain CHEBI:33019 with coefficient 1; the compound is diphosphate. Source location `http://rdf.rhea-db.org/16334 rh:products; http://rdf.rhea-db.org/16333_R rh:contains1 http://rdf.rhea-db.org/Participant_16333_compound_2106; rh:compound/rh:accession`
holo-[citrate lyase ACP] `RHEA-COMP:10158`	consumes Coefficient 1 of a whole generic citrate-lyase ACP polypeptide state. Its ChEBI reactive-part residue is not substituted for the complete carrier; no sequence-specific identity is asserted.	holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	RHEA:23788 Source assertion: RHEA:23789 substrates contain GENERIC:10158 with coefficient 1; the compound is holo-[citrate lyase ACP]. Source location `http://rdf.rhea-db.org/23789 rh:substrates; http://rdf.rhea-db.org/23788_L rh:contains1 http://rdf.rhea-db.org/Participant_23788_compound_10158; rh:compound/rh:accession` UniProtKB:P77390 Source assertion: The catalytic-activity Rhea cross-references include RHEA-COMP:10158, the whole ACP state represented by native Rhea Compound_10158. Source location `comments[1].reaction.reactionCrossReferences[database="Rhea"]`
acetate `CHEBI:30089`	consumes Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	RHEA:23788 Source assertion: RHEA:23789 substrates contain CHEBI:30089 with coefficient 1; the compound is acetate. Source location `http://rdf.rhea-db.org/23789 rh:substrates; http://rdf.rhea-db.org/23788_L rh:contains1 http://rdf.rhea-db.org/Participant_23788_compound_6051; rh:compound/rh:accession`
ATP(4-) `CHEBI:30616`	consumes Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	RHEA:23788 Source assertion: RHEA:23789 substrates contain CHEBI:30616 with coefficient 1; the compound is ATP. Source location `http://rdf.rhea-db.org/23789 rh:substrates; http://rdf.rhea-db.org/23788_L rh:contains1 http://rdf.rhea-db.org/Participant_23788_compound_6372; rh:compound/rh:accession`
holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	produces Coefficient 1 of a whole generic citrate-lyase ACP polypeptide state. Its ChEBI reactive-part residue is not substituted for the complete carrier; no sequence-specific identity is asserted.	acetyl-[citrate lyase ACP] `RHEA-COMP:13710`	RHEA:23788 Source assertion: RHEA:23789 products contain GENERIC:13710 with coefficient 1; the compound is acetyl-[citrate lyase ACP]. Source location `http://rdf.rhea-db.org/23789 rh:products; http://rdf.rhea-db.org/23788_R rh:contains1 http://rdf.rhea-db.org/Participant_23788_compound_13710; rh:compound/rh:accession` UniProtKB:P77390 Source assertion: The catalytic-activity Rhea cross-references include RHEA-COMP:13710, the whole ACP state represented by native Rhea Compound_13710. Source location `comments[1].reaction.reactionCrossReferences[database="Rhea"]`
holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	produces Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	adenosine 5'-monophosphate(2-) `CHEBI:456215`	RHEA:23788 Source assertion: RHEA:23789 products contain CHEBI:456215 with coefficient 1; the compound is AMP. Source location `http://rdf.rhea-db.org/23789 rh:products; http://rdf.rhea-db.org/23788_R rh:contains1 http://rdf.rhea-db.org/Participant_23788_compound_2590; rh:compound/rh:accession`
holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	produces Coefficient 1 in the selected activation direction, preserving the Rhea charge and stereochemical form.	diphosphate(3-) `CHEBI:33019`	RHEA:23788 Source assertion: RHEA:23789 products contain CHEBI:33019 with coefficient 1; the compound is diphosphate. Source location `http://rdf.rhea-db.org/23789 rh:products; http://rdf.rhea-db.org/23788_R rh:contains1 http://rdf.rhea-db.org/Participant_23788_compound_2106; rh:compound/rh:accession`
2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase `UniProtKB:P77231`	catalyzes CitG synthesizes the prosthetic-group precursor. The native reaction frame retains the obsolete EC-like name 2.7.8.25-RXN, but its current enzyme classification is EC 2.4.2.52.	3'-dephospho-CoA + ATP => 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine `RHEA:15118`	UniProtKB:P77231 Source assertion: The reviewed catalytic-activity comment assigns RHEA:15117 with experimental evidence ECO:0000269 from PMID:11042274. Source location `comments[1].reaction; reactionCrossReferences; evidences` PMID:11042274 Source assertion: CitG converts ATP and dephospho-CoA to adenine and triphosphoribosyl-dephospho-CoA, identifying the precursor proposed in the preceding study. Source location `Europe PMC core result[id="11042274"].abstractText`
Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase `UniProtKB:P0A6G5`	catalyzes CitX transfers the precursor to apo-ACP to produce holo-ACP. The separately demonstrated ATP-dependent adenylylation side reaction does not make AMP-ACP a pathway intermediate.	apo-[citrate lyase ACP] + 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA => holo-[citrate lyase ACP] + diphosphate `RHEA:16334`	UniProtKB:P0A6G5 Source assertion: The reviewed catalytic-activity comment assigns RHEA:16333 with experimental evidence ECO:0000269 from PMID:10924139 and PMID:11042274. Source location `comments[1].reaction; reactionCrossReferences; evidences` PMID:11042274 this precursor was transferred by CitX to apo-ACP, yielding holo-ACP. Source location `Europe PMC core result[id="11042274"].abstractText` PMID:10924139 Source assertion: Purified CitG and CitX convert apo-ACP to holo-ACP with ATP and dephospho-CoA; AMP-ACP cannot be converted further and is a side product. Source location `Europe PMC core result[id="10924139"].abstractText`
[Citrate [pro-3S]-lyase] ligase `UniProtKB:P77390`	catalyzes CitC acetylates the holo-ACP prosthetic-group thiol with acetate and ATP. Its exact sequence assignment is supported by reviewed annotation and an explicit curated direct-assay mapping; the inspected primary abstract establishes E. coli ligase activity, not independently verified purification of accession P77390.	holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	UniProtKB:P77390 Source assertion: The reviewed catalytic-activity comment assigns RHEA:23788 and EC 6.2.1.22. This comment has no inline experimental ECO tag. Source location `comments[1].reaction; reactionCrossReferences` UniProtKB:P77390 Source assertion: The GO:0008771 cross-reference has IDA:EcoCyc evidence ECO:0000314 citing PMID:6354265. This is an inspected database attribution, not independent inspection of the full experiment. Source location `uniProtKBCrossReferences[database="GO",id="GO:0008771"].properties; evidences` PMID:6354265 A ligase which acetylates the deacetyl[citrate lyase] in the presence of acetate and ATP Source location `Europe PMC core result[id="6354265"].abstractText, final sentence; abstract truncated at 250 words`
3'-dephospho-CoA + ATP => 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine `RHEA:15118`	part_of This activation reaction belongs to the retained E. coli core. Membership does not transfer the source diagram's other-species branches into this organism.	`MetaCyc:P2-PWY`	MetaCyc:P2-PWY Source assertion: P2-PWY explicitly lists 2.7.8.25-RXN as a pathway reaction. Source location `Pathway[@frameid="P2-PWY"]/reaction-list` RHEA:15117 Source assertion: RHEA:15118 has a seeAlso cross-reference to MetaCyc 2.7.8.25-RXN; RHEA:15117 lists RHEA:15118 as a directional reaction. Source location `http://rdf.rhea-db.org/15118 rdfs:seeAlso http://identifiers.org/biocyc/METACYC:2.7.8.25-RXN; http://rdf.rhea-db.org/15117 rh:directionalReaction`
apo-[citrate lyase ACP] + 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA => holo-[citrate lyase ACP] + diphosphate `RHEA:16334`	part_of This activation reaction belongs to the retained E. coli core. Membership does not transfer the source diagram's other-species branches into this organism.	`MetaCyc:P2-PWY`	MetaCyc:P2-PWY Source assertion: P2-PWY explicitly lists 2.7.7.61-RXN as a pathway reaction. Source location `Pathway[@frameid="P2-PWY"]/reaction-list` RHEA:16333 Source assertion: RHEA:16334 has a seeAlso cross-reference to MetaCyc 2.7.7.61-RXN; RHEA:16333 lists RHEA:16334 as a directional reaction. Source location `http://rdf.rhea-db.org/16334 rdfs:seeAlso http://identifiers.org/biocyc/METACYC:2.7.7.61-RXN; http://rdf.rhea-db.org/16333 rh:directionalReaction`
holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	part_of This activation reaction belongs to the retained E. coli core. Membership does not transfer the source diagram's other-species branches into this organism.	`MetaCyc:P2-PWY`	MetaCyc:P2-PWY Source assertion: P2-PWY explicitly lists CITC-RXN as a pathway reaction. Source location `Pathway[@frameid="P2-PWY"]/reaction-list` RHEA:23788 Source assertion: RHEA:23789 has a seeAlso cross-reference to MetaCyc CITC-RXN; RHEA:23788 lists RHEA:23789 as a directional reaction. Source location `http://rdf.rhea-db.org/23789 rdfs:seeAlso http://identifiers.org/biocyc/METACYC:CITC-RXN; http://rdf.rhea-db.org/23788 rh:directionalReaction`
3'-dephospho-CoA + ATP => 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine `RHEA:15118`	provides_input_for CitG supplies the precursor consumed by CitX; this is material transfer, not a claimed stable CitG–CitX complex or direct channeling interaction.	apo-[citrate lyase ACP] + 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA => holo-[citrate lyase ACP] + diphosphate `RHEA:16334`	RHEA:15117 Source assertion: RHEA:15118 produces CHEBI:61378. Source location `http://rdf.rhea-db.org/15118 rh:products; rh:contains1/rh:compound/rh:accession` RHEA:16333 Source assertion: RHEA:16334 consumes CHEBI:61378. Source location `http://rdf.rhea-db.org/16334 rh:substrates; rh:contains1/rh:compound/rh:accession`
apo-[citrate lyase ACP] + 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA => holo-[citrate lyase ACP] + diphosphate `RHEA:16334`	provides_input_for CitX supplies holo-ACP for CitC acetylation. The same whole carrier state is shared by the two reactions.	holo-[citrate lyase ACP] + acetate + ATP => acetyl-[citrate lyase ACP] + AMP + diphosphate `RHEA:23789`	RHEA:16333 Source assertion: RHEA:16334 produces Compound_10158 (GENERIC:10158), normalized as RHEA-COMP:10158. Source location `http://rdf.rhea-db.org/16334 rh:products; rh:contains1/rh:compound/rh:accession` RHEA:23788 Source assertion: RHEA:23789 consumes Compound_10158 (GENERIC:10158), normalized as RHEA-COMP:10158. Source location `http://rdf.rhea-db.org/23789 rh:substrates; rh:contains1/rh:compound/rh:accession`

References

MetaCyc:P2-PWY MetaCyc P2-PWY native citrate lyase activation pathway (source version: MetaCyc Pathway Tools 22.5; public XML retrieved 2026-10-10; exact response digest)
RHEA:15117 RHEA:15117 and activation direction RHEA:15118 (source version: Rhea 142, 2026-09-02; digest covers the complete compressed official RDF)
RHEA:16333 RHEA:16333 and activation direction RHEA:16334 (source version: Rhea 142, 2026-09-02; digest covers the complete compressed official RDF)
RHEA:23788 RHEA:23788 and activation direction RHEA:23789 (source version: Rhea 142, 2026-09-02; digest covers the complete compressed official RDF)
UniProtKB:P77231 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase — reviewed UniProtKB record (source version: Reviewed entry version 152; sequence version 1; retrieved 2026-10-10; exact JSON response digest)
UniProtKB:P0A6G5 Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase — reviewed UniProtKB record (source version: Reviewed entry version 120; sequence version 1; retrieved 2026-10-10; exact JSON response digest)
UniProtKB:P77390 [Citrate [pro-3S]-lyase] ligase — reviewed UniProtKB record (source version: Reviewed entry version 167; sequence version 2; retrieved 2026-10-10; exact JSON response digest)
PMID:10924139 Biosynthesis of the prosthetic group of citrate lyase. (source version: Primary abstract in Europe PMC core; DOI 10.1021/bi000401r; retrieved 2026-10-10; digest covers exact JSON response)
PMID:11042274 Identification of triphosphoribosyl-dephospho-CoA as precursor of the citrate lyase prosthetic group. (source version: Primary abstract in Europe PMC core; DOI 10.1016/s0014-5793(00)02105-0; retrieved 2026-10-10; digest covers exact JSON response)
PMID:6354265 Purification and properties of citrate lyase from Escherichia coli. (source version: Primary abstract in Europe PMC core; DOI 10.1021/bi00289a008; retrieved 2026-10-10; digest covers exact JSON response; abstract truncated at 250 words)

Curation history

2026-10-10T23:03:14Z claude-code
- CREATE — Add citrate lyase activation with explicit carrier states and CitG, CitX and CitC evidence
  Curated MetaCyc:P2-PWY from native pathway objects, reviewed UniProt, directed Rhea chemistry and inspected primary evidence. Records three reactions, distinct generic apo/holo/acetyl carrier states, 22 edges and 36 evidence blocks. Narrow native/closed schema, independently verified identifiers, all 14 Rhea side occurrences and elemental/charge balance, literal quotes and target KGX metadata checks passed before this event. CitC full-text access and sequence-versus-generic-carrier limits remain explicit; unsupported assemblies, metals and alternate taxon branches were excluded. Full-corpus gates and independent review are recorded separately in this batch research directory.
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2026-10-10T23:04:04Z codex
- GENERAL — Correct the creation-history actor attribution
  The preceding immutable history record history/records/citrate-lyase-activation/2026-10-10T230314Z-claude-code-9e9c5c.yaml inherited the scaffolder default actor name claude-code. The actual curator was Codex (gpt-6), as its model and agent_tool fields already indicate. This append-only correction preserves the original event and corrects the actor name to codex. The pathway data and scientific assertions are unchanged.
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