UMP biosynthesis I

MetaCyc:PWY-5686

UMP biosynthesis I uses glutamine-dependent carbamoyl-phosphate synthase to form carbamoyl phosphate, condenses it with L-aspartate, cyclizes and oxidizes the pyrimidine ring to orotate, transfers a phosphoribosyl group to form orotidine 5'-phosphate, and decarboxylates that intermediate to UMP.

Pathway type

nucleotide biosynthesis

Organism scope

Escherichia coli NCBITaxon:562

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Pathway network

44 nodes · 54 asserted directed edges

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Chemical / cofactor
Protein / complex
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Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Escherichia coli `NCBITaxon:562`	taxon
carbamoyl-phosphate synthase (glutamine-hydrolysing) `EC:6.3.5.5`	molecular activity
aspartate carbamoyltransferase `EC:2.1.3.2`	molecular activity
dihydroorotase `EC:3.5.2.3`	molecular activity
dihydroorotate dehydrogenase (quinone) `EC:1.3.5.2`	molecular activity
orotate phosphoribosyltransferase `EC:2.4.2.10`	molecular activity
orotidine-5'-phosphate decarboxylase `EC:4.1.1.23`	molecular activity
hydrogencarbonate `CHEBI:17544`	small molecule
L-glutamine `CHEBI:58359`	small molecule
ATP `CHEBI:30616`	small molecule
H2O `CHEBI:15377`	small molecule
carbamoyl phosphate `CHEBI:58228`	small molecule
L-glutamate `CHEBI:29985`	small molecule
ADP `CHEBI:456216`	small molecule
phosphate `CHEBI:43474`	small molecule
H(+) `CHEBI:15378`	small molecule
L-aspartate `CHEBI:29991`	small molecule
N-carbamoyl-L-aspartate `CHEBI:32814`	small molecule
(S)-dihydroorotate `CHEBI:30864`	small molecule
a quinone `CHEBI:132124`	small molecule
orotate `CHEBI:30839`	small molecule
a quinol `CHEBI:24646`	small molecule
5-phospho-alpha-D-ribose 1-diphosphate `CHEBI:58017`	small molecule
orotidine 5'-phosphate `CHEBI:57538`	small molecule
diphosphate `CHEBI:33019`	small molecule
UMP `CHEBI:57865`	small molecule
CO2 `CHEBI:16526`	small molecule
Carbamoyl phosphate synthase large chain `UniProtKB:P00968`	protein
magnesium(2+) `CHEBI:18420`	cofactor
manganese(2+) `CHEBI:29035`	cofactor
Dihydroorotase `UniProtKB:P05020`	protein
zinc(2+) `CHEBI:29105`	cofactor
Orotidine 5'-phosphate decarboxylase `UniProtKB:P08244`	protein
Carbamoyl phosphate synthase small chain `UniProtKB:P0A6F1`	protein
Aspartate carbamoyltransferase catalytic subunit `UniProtKB:P0A786`	protein
Dihydroorotate dehydrogenase (quinone) `UniProtKB:P0A7E1`	protein
plasma membrane `GO:0005886`	cellular component
FMN(3-) `CHEBI:58210`	cofactor
Orotate phosphoribosyltransferase `UniProtKB:P0A7E3`	protein
carbamoyl-phosphate synthase reaction `RHEA:18634`	reaction	left to right
aspartate carbamoyltransferase reaction `RHEA:20014`	reaction	left to right
dihydroorotase reaction `RHEA:24298`	reaction	right to left
dihydroorotate dehydrogenase quinone reaction `RHEA:30188`	reaction	left to right
orotate phosphoribosyltransferase reaction `RHEA:10382`	reaction	right to left
orotidine-5'-phosphate decarboxylase reaction `RHEA:11597`	reaction	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
H2O `CHEBI:15377`	consumes	carbamoyl-phosphate synthase reaction `RHEA:18634`	RHEA:18634 Source assertion: Rhea lists CHEBI:15377 on the substrates side of RHEA:18634 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/18634']/rh:substrates; rh:contains/rh:compound/rh:accession`
hydrogencarbonate `CHEBI:17544`	consumes	carbamoyl-phosphate synthase reaction `RHEA:18634`	RHEA:18634 Source assertion: Rhea lists CHEBI:17544 on the substrates side of RHEA:18634 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/18634']/rh:substrates; rh:contains/rh:compound/rh:accession`
ATP `CHEBI:30616`	consumes	carbamoyl-phosphate synthase reaction `RHEA:18634`	RHEA:18634 Source assertion: Rhea lists CHEBI:30616 on the substrates side of RHEA:18634 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/18634']/rh:substrates; rh:contains/rh:compound/rh:accession`
L-glutamine `CHEBI:58359`	consumes	carbamoyl-phosphate synthase reaction `RHEA:18634`	RHEA:18634 Source assertion: Rhea lists CHEBI:58359 on the substrates side of RHEA:18634 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/18634']/rh:substrates; rh:contains/rh:compound/rh:accession`
carbamoyl-phosphate synthase reaction `RHEA:18634`	produces	H(+) `CHEBI:15378`	RHEA:18634 Source assertion: Rhea lists CHEBI:15378 on the products side of RHEA:18634 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/18634']/rh:products; rh:contains/rh:compound/rh:accession`
carbamoyl-phosphate synthase reaction `RHEA:18634`	produces	L-glutamate `CHEBI:29985`	RHEA:18634 Source assertion: Rhea lists CHEBI:29985 on the products side of RHEA:18634 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/18634']/rh:products; rh:contains/rh:compound/rh:accession`
carbamoyl-phosphate synthase reaction `RHEA:18634`	produces	phosphate `CHEBI:43474`	RHEA:18634 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:18634 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/18634']/rh:products; rh:contains/rh:compound/rh:accession`
carbamoyl-phosphate synthase reaction `RHEA:18634`	produces	ADP `CHEBI:456216`	RHEA:18634 Source assertion: Rhea lists CHEBI:456216 on the products side of RHEA:18634 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/18634']/rh:products; rh:contains/rh:compound/rh:accession`
carbamoyl-phosphate synthase reaction `RHEA:18634`	produces	carbamoyl phosphate `CHEBI:58228`	RHEA:18634 Source assertion: Rhea lists CHEBI:58228 on the products side of RHEA:18634 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/18634']/rh:products; rh:contains/rh:compound/rh:accession`
L-aspartate `CHEBI:29991`	consumes	aspartate carbamoyltransferase reaction `RHEA:20014`	RHEA:20014 Source assertion: Rhea lists CHEBI:29991 on the substrates side of RHEA:20014 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/20014']/rh:substrates; rh:contains/rh:compound/rh:accession`
carbamoyl phosphate `CHEBI:58228`	consumes	aspartate carbamoyltransferase reaction `RHEA:20014`	RHEA:20014 Source assertion: Rhea lists CHEBI:58228 on the substrates side of RHEA:20014 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/20014']/rh:substrates; rh:contains/rh:compound/rh:accession`
aspartate carbamoyltransferase reaction `RHEA:20014`	produces	H(+) `CHEBI:15378`	RHEA:20014 Source assertion: Rhea lists CHEBI:15378 on the products side of RHEA:20014 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/20014']/rh:products; rh:contains/rh:compound/rh:accession`
aspartate carbamoyltransferase reaction `RHEA:20014`	produces	N-carbamoyl-L-aspartate `CHEBI:32814`	RHEA:20014 Source assertion: Rhea lists CHEBI:32814 on the products side of RHEA:20014 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/20014']/rh:products; rh:contains/rh:compound/rh:accession`
aspartate carbamoyltransferase reaction `RHEA:20014`	produces	phosphate `CHEBI:43474`	RHEA:20014 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:20014 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/20014']/rh:products; rh:contains/rh:compound/rh:accession`
H(+) `CHEBI:15378`	consumes	dihydroorotase reaction `RHEA:24298`	RHEA:24298 Source assertion: Rhea lists CHEBI:15378 on the substrates side of RHEA:24298 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24298']/rh:substrates; rh:contains/rh:compound/rh:accession`
N-carbamoyl-L-aspartate `CHEBI:32814`	consumes	dihydroorotase reaction `RHEA:24298`	RHEA:24298 Source assertion: Rhea lists CHEBI:32814 on the substrates side of RHEA:24298 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24298']/rh:substrates; rh:contains/rh:compound/rh:accession`
dihydroorotase reaction `RHEA:24298`	produces	H2O `CHEBI:15377`	RHEA:24298 Source assertion: Rhea lists CHEBI:15377 on the products side of RHEA:24298 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24298']/rh:products; rh:contains/rh:compound/rh:accession`
dihydroorotase reaction `RHEA:24298`	produces	(S)-dihydroorotate `CHEBI:30864`	RHEA:24298 Source assertion: Rhea lists CHEBI:30864 on the products side of RHEA:24298 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24298']/rh:products; rh:contains/rh:compound/rh:accession`
a quinone `CHEBI:132124`	consumes	dihydroorotate dehydrogenase quinone reaction `RHEA:30188`	RHEA:30188 Source assertion: Rhea lists CHEBI:132124 on the substrates side of RHEA:30188 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/30188']/rh:substrates; rh:contains/rh:compound/rh:accession`
(S)-dihydroorotate `CHEBI:30864`	consumes	dihydroorotate dehydrogenase quinone reaction `RHEA:30188`	RHEA:30188 Source assertion: Rhea lists CHEBI:30864 on the substrates side of RHEA:30188 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/30188']/rh:substrates; rh:contains/rh:compound/rh:accession`
dihydroorotate dehydrogenase quinone reaction `RHEA:30188`	produces	a quinol `CHEBI:24646`	RHEA:30188 Source assertion: Rhea lists CHEBI:24646 on the products side of RHEA:30188 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/30188']/rh:products; rh:contains/rh:compound/rh:accession`
dihydroorotate dehydrogenase quinone reaction `RHEA:30188`	produces	orotate `CHEBI:30839`	RHEA:30188 Source assertion: Rhea lists CHEBI:30839 on the products side of RHEA:30188 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/30188']/rh:products; rh:contains/rh:compound/rh:accession`
orotate `CHEBI:30839`	consumes	orotate phosphoribosyltransferase reaction `RHEA:10382`	RHEA:10382 Source assertion: Rhea lists CHEBI:30839 on the substrates side of RHEA:10382 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/10382']/rh:substrates; rh:contains/rh:compound/rh:accession`
5-phospho-alpha-D-ribose 1-diphosphate `CHEBI:58017`	consumes	orotate phosphoribosyltransferase reaction `RHEA:10382`	RHEA:10382 Source assertion: Rhea lists CHEBI:58017 on the substrates side of RHEA:10382 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/10382']/rh:substrates; rh:contains/rh:compound/rh:accession`
orotate phosphoribosyltransferase reaction `RHEA:10382`	produces	diphosphate `CHEBI:33019`	RHEA:10382 Source assertion: Rhea lists CHEBI:33019 on the products side of RHEA:10382 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/10382']/rh:products; rh:contains/rh:compound/rh:accession`
orotate phosphoribosyltransferase reaction `RHEA:10382`	produces	orotidine 5'-phosphate `CHEBI:57538`	RHEA:10382 Source assertion: Rhea lists CHEBI:57538 on the products side of RHEA:10382 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/10382']/rh:products; rh:contains/rh:compound/rh:accession`
H(+) `CHEBI:15378`	consumes	orotidine-5'-phosphate decarboxylase reaction `RHEA:11597`	RHEA:11597 Source assertion: Rhea lists CHEBI:15378 on the substrates side of RHEA:11597 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/11597']/rh:substrates; rh:contains/rh:compound/rh:accession`
orotidine 5'-phosphate `CHEBI:57538`	consumes	orotidine-5'-phosphate decarboxylase reaction `RHEA:11597`	RHEA:11597 Source assertion: Rhea lists CHEBI:57538 on the substrates side of RHEA:11597 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/11597']/rh:substrates; rh:contains/rh:compound/rh:accession`
orotidine-5'-phosphate decarboxylase reaction `RHEA:11597`	produces	CO2 `CHEBI:16526`	RHEA:11597 Source assertion: Rhea lists CHEBI:16526 on the products side of RHEA:11597 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/11597']/rh:products; rh:contains/rh:compound/rh:accession`
orotidine-5'-phosphate decarboxylase reaction `RHEA:11597`	produces	UMP `CHEBI:57865`	RHEA:11597 Source assertion: Rhea lists CHEBI:57865 on the products side of RHEA:11597 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/11597']/rh:products; rh:contains/rh:compound/rh:accession`
carbamoyl-phosphate synthase (glutamine-hydrolysing) `EC:6.3.5.5`	catalyzes	carbamoyl-phosphate synthase reaction `RHEA:18634`	RHEA:18633 Source assertion: Rhea associates EC:6.3.5.5 with the reaction family containing RHEA:18634. Source location `rh:ec`
aspartate carbamoyltransferase `EC:2.1.3.2`	catalyzes	aspartate carbamoyltransferase reaction `RHEA:20014`	RHEA:20013 Source assertion: Rhea associates EC:2.1.3.2 with the reaction family containing RHEA:20014. Source location `rh:ec`
dihydroorotase `EC:3.5.2.3`	catalyzes	dihydroorotase reaction `RHEA:24298`	RHEA:24296 Source assertion: Rhea associates EC:3.5.2.3 with the reaction family containing RHEA:24298. Source location `rh:ec`
dihydroorotate dehydrogenase (quinone) `EC:1.3.5.2`	catalyzes	dihydroorotate dehydrogenase quinone reaction `RHEA:30188`	RHEA:30187 Source assertion: Rhea associates EC:1.3.5.2 with the reaction family containing RHEA:30188. Source location `rh:ec`
orotate phosphoribosyltransferase `EC:2.4.2.10`	catalyzes	orotate phosphoribosyltransferase reaction `RHEA:10382`	RHEA:10380 Source assertion: Rhea associates EC:2.4.2.10 with the reaction family containing RHEA:10382. Source location `rh:ec`
orotidine-5'-phosphate decarboxylase `EC:4.1.1.23`	catalyzes	orotidine-5'-phosphate decarboxylase reaction `RHEA:11597`	RHEA:11596 Source assertion: Rhea associates EC:4.1.1.23 with the reaction family containing RHEA:11597. Source location `rh:ec`
orotate phosphoribosyltransferase reaction `RHEA:10382`	precedes	orotidine-5'-phosphate decarboxylase reaction `RHEA:11597`	MetaCyc:PWY-5686 Source assertion: MetaCyc pathway ordering places RHEA:10382 before RHEA:11597 in this pathway. Shared intermediate: CHEBI:57538. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
carbamoyl-phosphate synthase reaction `RHEA:18634`	precedes	aspartate carbamoyltransferase reaction `RHEA:20014`	MetaCyc:PWY-5686 Source assertion: MetaCyc pathway ordering places RHEA:18634 before RHEA:20014 in this pathway. Shared intermediate: CHEBI:58228. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
aspartate carbamoyltransferase reaction `RHEA:20014`	precedes	dihydroorotase reaction `RHEA:24298`	MetaCyc:PWY-5686 Source assertion: MetaCyc pathway ordering places RHEA:20014 before RHEA:24298 in this pathway. Shared intermediate: CHEBI:32814. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
dihydroorotase reaction `RHEA:24298`	precedes	dihydroorotate dehydrogenase quinone reaction `RHEA:30188`	MetaCyc:PWY-5686 Source assertion: MetaCyc pathway ordering places RHEA:24298 before RHEA:30188 in this pathway. Shared intermediate: CHEBI:30864. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
dihydroorotate dehydrogenase quinone reaction `RHEA:30188`	precedes	orotate phosphoribosyltransferase reaction `RHEA:10382`	MetaCyc:PWY-5686 Source assertion: MetaCyc pathway ordering places RHEA:30188 before RHEA:10382 in this pathway. Shared intermediate: CHEBI:30839. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
Carbamoyl phosphate synthase large chain `UniProtKB:P00968`	enables	carbamoyl-phosphate synthase reaction `RHEA:18634`	UniProtKB:P00968 Source assertion: UniProt annotates UniProtKB:P00968 with catalytic activity for the reaction family of RHEA:18634. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Carbamoyl phosphate synthase large chain `UniProtKB:P00968`	has_cofactor	magnesium(2+) `CHEBI:18420`	UniProtKB:P00968 Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:P00968 (evidence: ECO:0000255). Binds 4 Mg(2+) or Mn(2+) ions per subunit. Source location `comments[commentType='COFACTOR'].cofactors and note`
Carbamoyl phosphate synthase large chain `UniProtKB:P00968`	has_cofactor	manganese(2+) `CHEBI:29035`	UniProtKB:P00968 Source assertion: UniProt lists CHEBI:29035 as a cofactor of UniProtKB:P00968 (evidence: ECO:0000255, ECO:0000269). Binds 4 Mg(2+) or Mn(2+) ions per subunit. Source location `comments[commentType='COFACTOR'].cofactors and note`
Dihydroorotase `UniProtKB:P05020`	enables	dihydroorotase reaction `RHEA:24298`	UniProtKB:P05020 Source assertion: UniProt annotates UniProtKB:P05020 with catalytic activity for the reaction family of RHEA:24298. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Dihydroorotase `UniProtKB:P05020`	has_cofactor	zinc(2+) `CHEBI:29105`	UniProtKB:P05020 Source assertion: UniProt lists CHEBI:29105 as a cofactor of UniProtKB:P05020 (evidence: ECO:0000255, ECO:0000269). Binds 2 Zn(2+) ions per subunit (PubMed:11401542, PubMed:15826651, PubMed:6142052). In vitro, can also use Co(2+) or Cd(2+) (PubMed:15610022, PubMed:1671037). Source location `comments[commentType='COFACTOR'].cofactors and note`
Orotidine 5'-phosphate decarboxylase `UniProtKB:P08244`	enables	orotidine-5'-phosphate decarboxylase reaction `RHEA:11597`	UniProtKB:P08244 Source assertion: UniProt annotates UniProtKB:P08244 with catalytic activity for the reaction family of RHEA:11597. Annotation evidence: curated source annotation; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Carbamoyl phosphate synthase small chain `UniProtKB:P0A6F1`	enables	carbamoyl-phosphate synthase reaction `RHEA:18634`	UniProtKB:P0A6F1 Source assertion: UniProt annotates UniProtKB:P0A6F1 with catalytic activity for the reaction family of RHEA:18634. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Aspartate carbamoyltransferase catalytic subunit `UniProtKB:P0A786`	enables	aspartate carbamoyltransferase reaction `RHEA:20014`	UniProtKB:P0A786 Source assertion: UniProt annotates UniProtKB:P0A786 with catalytic activity for the reaction family of RHEA:20014. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Dihydroorotate dehydrogenase (quinone) `UniProtKB:P0A7E1`	enables	dihydroorotate dehydrogenase quinone reaction `RHEA:30188`	UniProtKB:P0A7E1 Source assertion: UniProt annotates UniProtKB:P0A7E1 with catalytic activity for the reaction family of RHEA:30188. Annotation evidence: ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Dihydroorotate dehydrogenase (quinone) `UniProtKB:P0A7E1`	located_in	plasma membrane `GO:0005886`	UniProtKB:P0A7E1 Source assertion: UniProt locates UniProtKB:P0A7E1 in Cell membrane (SL-0039, mapped by UniProt to GO:0005886). Annotation evidence: curated source annotation. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Dihydroorotate dehydrogenase (quinone) `UniProtKB:P0A7E1`	has_cofactor	FMN(3-) `CHEBI:58210`	UniProtKB:P0A7E1 Source assertion: UniProt lists CHEBI:58210 as a cofactor of UniProtKB:P0A7E1 (evidence: ECO:0000269). Binds 1 FMN per subunit. Source location `comments[commentType='COFACTOR'].cofactors and note`
Orotate phosphoribosyltransferase `UniProtKB:P0A7E3`	enables	orotate phosphoribosyltransferase reaction `RHEA:10382`	UniProtKB:P0A7E3 Source assertion: UniProt annotates UniProtKB:P0A7E3 with catalytic activity for the reaction family of RHEA:10382. Annotation evidence: ECO:0000255; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Orotate phosphoribosyltransferase `UniProtKB:P0A7E3`	has_cofactor	magnesium(2+) `CHEBI:18420`	UniProtKB:P0A7E3 Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:P0A7E3 (evidence: ECO:0000255). Source location `comments[commentType='COFACTOR'].cofactors and note`

References

MetaCyc:PWY-5686 MetaCyc UMP biosynthesis I pathway (source version: 22.5)
RHEA:18633 Rhea carbamoyl-phosphate synthase reaction (source version: 139)
RHEA:18634 Rhea carbamoyl-phosphate-forming direction (source version: 139)
RHEA:20013 Rhea aspartate carbamoyltransferase reaction (source version: 139)
RHEA:20014 Rhea N-carbamoyl-L-aspartate-forming direction (source version: 139)
RHEA:24296 Rhea dihydroorotase reaction (source version: 139)
RHEA:24298 Rhea dihydroorotate-forming direction (source version: 139)
RHEA:30187 Rhea dihydroorotate dehydrogenase quinone reaction (source version: 139)
RHEA:30188 Rhea orotate-forming direction (source version: 139)
RHEA:10380 Rhea orotate phosphoribosyltransferase reaction (source version: 139)
RHEA:10382 Rhea orotidine 5'-phosphate-forming direction (source version: 139)
RHEA:11596 Rhea orotidine-5'-phosphate decarboxylase reaction (source version: 139)
RHEA:11597 Rhea UMP-forming direction (source version: 139)
UniProtKB:P00968 UniProtKB:P00968 authority record (source version: 2026_03)
UniProtKB:P05020 UniProtKB:P05020 authority record (source version: 2026_03)
UniProtKB:P08244 UniProtKB:P08244 authority record (source version: 2026_03)
UniProtKB:P0A6F1 UniProtKB:P0A6F1 authority record (source version: 2026_03)
UniProtKB:P0A786 UniProtKB:P0A786 authority record (source version: 2026_03)
UniProtKB:P0A7E1 UniProtKB:P0A7E1 authority record (source version: 2026_03)
UniProtKB:P0A7E3 UniProtKB:P0A7E3 authority record (source version: 2026_03)

Curation history

2026-10-05T06:25:52Z codex
- EDIT — Review causal chemistry and add sourced molecular context
  Reviewed the entire record against MetaCyc 22.5 native pathway XML (including nested pathways), Rhea 139 explicit sides and directions, ENZYME 02-Sep-2026, PubMed records, and reviewed UniProt 2026_03 annotations within the record's taxon scope. Replaced generated structured-source quotations with source_assertion and source_locator. Typed molecular activities and participants. Reaction count 6 to 6; 0 directed-ID corrections; 7 protein annotations, 5 cofactor annotations and 1 sourced locations. All Rhea reaction substrate/product sets checked against independent Rhea authority; native pathway leaf coverage and source exceptions recorded in reports/causal_graph_review/metacyc-review.json. Full source URLs, versions and byte hashes are in metacyc-sources.json. All 50 MetaCyc records passed native schema validation; six targeted chemistry/evidence regression tests passed. Missing protein or cofactor annotations are not treated as biological absence; qualifications and exclusions are preserved in the ledger.
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