glyoxylate assimilation

MetaCyc:PWY-5744

The complete native 13-object glyoxylate-assimilation cycle of Chloroflexus aurantiacus converts glyoxylate plus inorganic carbon to pyruvate while regenerating its acetyl-CoA/propionyl-CoA carrier pool. The acetyl-CoA-to-propionyl-CoA regeneration branch and all five dedicated assimilation reactions are retained, including CO2/bicarbonate equilibration and two whole protein-bound biotin half-reactions. The separate first 3-hydroxypropionate cycle that generates glyoxylate is outside this record. The five dedicated transformations were reconstituted with four enzymes from OK-70-fl/DSM636; current J-10-fl genome annotations and 2024 BC1 work are distinguished, with CT function retained at its inference tier. Mcl is a hexamer; MCR and the three dedicated hydratase/isomerase enzymes are dimers. PCS has an unresolved three-or-four-subunit estimate, and no fixed whole-ACC assembly is asserted. Current (2R,3S)-beta-methylmalyl-CoA agrees with primary Figure 1; the older native CPD-9409 structure encodes opposite acyl stereocenters despite its matching label and is not accepted as an exact structure mapping. Native citramalyl-CoA leaves CoA stereocenters unspecified; current stereospecific CoA chemistry is retained separately. Physiological MCR metal identity remains unresolved. No carbonic-anhydrase identity, transcriptional regulator or transporter is invented; condition-dependent induction and biochemical inhibition do not establish regulatory circuitry.

Pathway type

glyoxylate assimilation cycle

Organism scope

Chloroflexus aurantiacus NCBITaxon:1108

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Pathway network

61 nodes · 107 asserted directed edges

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Chloroflexus aurantiacus `NCBITaxon:1108`	taxon
pyruvate `CHEBI:15361`	small molecule
water `CHEBI:15377`	small molecule
hydron `CHEBI:15378`	small molecule
3-hydroxypropionate `CHEBI:16510`	small molecule
carbon dioxide `CHEBI:16526`	small molecule
hydrogencarbonate `CHEBI:17544`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
diphosphate(3-) `CHEBI:33019`	small molecule
3-oxopropanoate `CHEBI:33190`	small molecule
glyoxylate `CHEBI:36655`	small molecule
hydrogenphosphate `CHEBI:43474`	small molecule
adenosine 5'-monophosphate(2-) `CHEBI:456215`	small molecule
ADP(3-) `CHEBI:456216`	small molecule
coenzyme A(4-) `CHEBI:57287`	small molecule
acetyl-CoA(4-) `CHEBI:57288`	small molecule
acryloyl-CoA(4-) `CHEBI:57367`	small molecule
malonyl-CoA(5-) `CHEBI:57384`	small molecule
propionyl-CoA(4-) `CHEBI:57392`	small molecule
NADPH(4-) `CHEBI:57783`	small molecule
NADP(3-) `CHEBI:58349`	small molecule
3-hydroxypropanoyl-CoA(4-) `CHEBI:58528`	small molecule
(3S)-citramalyl-CoA(5-) `CHEBI:58668`	small molecule
L-erythro-3-methylmalyl-CoA(5-) `CHEBI:75634`	small molecule
mesaconyl-CoA(5-) `CHEBI:75635`	small molecule
3-methylfumaryl-CoA(5-) `CHEBI:75636`	small molecule
N(6)-biotinyl-L-lysyl-[protein] `RHEA-COMP:10505`	protein
N(6)-carboxybiotinyl-L-lysyl-[protein] `RHEA-COMP:10506`	protein
magnesium(2+) `CHEBI:18420`	cofactor
manganese(2+) `CHEBI:29035`	cofactor
zinc(2+) `CHEBI:29105`	cofactor
biotinate `CHEBI:57586`	cofactor
potassium(1+) `CHEBI:29103`	cofactor
hydroxylamine `CHEBI:15429`	small molecule
biotin carboxylase `UniProtKB:A9W9X0`	protein
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha `UniProtKB:A9WBQ8`	protein
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta `UniProtKB:A9WBQ9`	protein
Malonyl-CoA reductase `UniProtKB:Q6QQP7`	protein
acetate--CoA ligase `UniProtKB:Q8VRG6`	protein
Malyl-CoA/beta-methylmalyl-CoA/citramalyl-CoA lyase `UniProtKB:S5N020`	protein
Beta-methylmalyl-CoA dehydratase `UniProtKB:A9WC34`	protein
2-methylfumaryl-CoA isomerase `UniProtKB:A9WC36`	protein
Mesaconyl-C(4)-CoA hydratase `UniProtKB:A9WC41`	protein
malonyl CoA reductase `MetaCyc:CPLX-7550`	complex
L-malyl CoA lyase `MetaCyc:CPLX-7549`	complex
beta-methylmalyl-CoA dehydratase `MetaCyc:CPLX-8468`	complex
mesaconyl-C1-CoA-C4-CoA transferase `MetaCyc:CPLX-8466`	complex
mesaconyl-C4-CoA hydratase `MetaCyc:CPLX-8467`	complex
CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	reaction	right to left
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	molecular activity	left to right
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	molecular activity	left to right
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	molecular activity	right to left
3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	molecular activity	right to left
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	molecular activity	left to right
3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	molecular activity	left to right
acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	molecular activity	right to left
propanoyl-CoA + glyoxylate => (2R,3S)-beta-methylmalyl-CoA `RHEA:38261`	molecular activity	right to left
(2R,3S)-beta-methylmalyl-CoA => 2-methylfumaryl-CoA + H2O `RHEA:38264`	molecular activity	left to right
2-methylfumaryl-CoA => 3-methylfumaryl-CoA `RHEA:38268`	molecular activity	left to right
3-methylfumaryl-CoA + H2O => (3S)-citramalyl-CoA `RHEA:38273`	molecular activity	right to left
(3S)-citramalyl-CoA => pyruvate + acetyl-CoA `RHEA:22613`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
carbon dioxide `CHEBI:16526`	consumes Stoichiometric coefficient 1.	CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	RHEA:10750 Source assertion: Rhea directed reaction 10750 places CHEBI:16526 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/10750 rh:substrates http://rdf.rhea-db.org/10748_R; http://rdf.rhea-db.org/10748_R rh:contains1 http://rdf.rhea-db.org/Participant_10748_compound_4391; http://rdf.rhea-db.org/Participant_10748_compound_4391 rh:compound http://rdf.rhea-db.org/Compound_4391; rh:accession`
water `CHEBI:15377`	consumes Stoichiometric coefficient 1.	CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	RHEA:10750 Source assertion: Rhea directed reaction 10750 places CHEBI:15377 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/10750 rh:substrates http://rdf.rhea-db.org/10748_R; http://rdf.rhea-db.org/10748_R rh:contains1 http://rdf.rhea-db.org/Participant_10748_compound_1283; http://rdf.rhea-db.org/Participant_10748_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; rh:accession`
CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	produces Stoichiometric coefficient 1.	hydrogencarbonate `CHEBI:17544`	RHEA:10750 Source assertion: Rhea directed reaction 10750 places CHEBI:17544 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/10750 rh:products http://rdf.rhea-db.org/10748_L; http://rdf.rhea-db.org/10748_L rh:contains1 http://rdf.rhea-db.org/Participant_10748_compound_1123; http://rdf.rhea-db.org/Participant_10748_compound_1123 rh:compound http://rdf.rhea-db.org/Compound_1123; rh:accession`
CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	produces Stoichiometric coefficient 1.	hydron `CHEBI:15378`	RHEA:10750 Source assertion: Rhea directed reaction 10750 places CHEBI:15378 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/10750 rh:products http://rdf.rhea-db.org/10748_L; http://rdf.rhea-db.org/10748_L rh:contains1 http://rdf.rhea-db.org/Participant_10748_compound_3249; http://rdf.rhea-db.org/Participant_10748_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] `RHEA-COMP:10505`	consumes Stoichiometric coefficient 1.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:13502 Source assertion: Rhea directed reaction 13502 places RHEA-COMP:10505 on its substrates side with coefficient 1. This is a whole generic protein-bearing carrier state, not an isolated lysine residue. Source location `http://rdf.rhea-db.org/13502 rh:substrates http://rdf.rhea-db.org/13501_L; http://rdf.rhea-db.org/13501_L rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_10505; http://rdf.rhea-db.org/Participant_13501_compound_10505 rh:compound http://rdf.rhea-db.org/Compound_10505; rh:accession`
hydrogencarbonate `CHEBI:17544`	consumes Stoichiometric coefficient 1.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:13502 Source assertion: Rhea directed reaction 13502 places CHEBI:17544 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:substrates http://rdf.rhea-db.org/13501_L; http://rdf.rhea-db.org/13501_L rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_1123; http://rdf.rhea-db.org/Participant_13501_compound_1123 rh:compound http://rdf.rhea-db.org/Compound_1123; rh:accession`
ATP(4-) `CHEBI:30616`	consumes Stoichiometric coefficient 1.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:13502 Source assertion: Rhea directed reaction 13502 places CHEBI:30616 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:substrates http://rdf.rhea-db.org/13501_L; http://rdf.rhea-db.org/13501_L rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_6372; http://rdf.rhea-db.org/Participant_13501_compound_6372 rh:compound http://rdf.rhea-db.org/Compound_6372; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	produces Stoichiometric coefficient 1.	N(6)-carboxybiotinyl-L-lysyl-[protein] `RHEA-COMP:10506`	RHEA:13502 Source assertion: Rhea directed reaction 13502 places RHEA-COMP:10506 on its products side with coefficient 1. This is a whole generic protein-bearing carrier state, not an isolated lysine residue. Source location `http://rdf.rhea-db.org/13502 rh:products http://rdf.rhea-db.org/13501_R; http://rdf.rhea-db.org/13501_R rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_10506; http://rdf.rhea-db.org/Participant_13501_compound_10506 rh:compound http://rdf.rhea-db.org/Compound_10506; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	produces Stoichiometric coefficient 1.	ADP(3-) `CHEBI:456216`	RHEA:13502 Source assertion: Rhea directed reaction 13502 places CHEBI:456216 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:products http://rdf.rhea-db.org/13501_R; http://rdf.rhea-db.org/13501_R rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_2710; http://rdf.rhea-db.org/Participant_13501_compound_2710 rh:compound http://rdf.rhea-db.org/Compound_2710; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	produces Stoichiometric coefficient 1.	hydrogenphosphate `CHEBI:43474`	RHEA:13502 Source assertion: Rhea directed reaction 13502 places CHEBI:43474 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:products http://rdf.rhea-db.org/13501_R; http://rdf.rhea-db.org/13501_R rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_5518; http://rdf.rhea-db.org/Participant_13501_compound_5518 rh:compound http://rdf.rhea-db.org/Compound_5518; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	produces Stoichiometric coefficient 1.	hydron `CHEBI:15378`	RHEA:13502 Source assertion: Rhea directed reaction 13502 places CHEBI:15378 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:products http://rdf.rhea-db.org/13501_R; http://rdf.rhea-db.org/13501_R rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_3249; http://rdf.rhea-db.org/Participant_13501_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
(3S)-citramalyl-CoA(5-) `CHEBI:58668`	consumes Stoichiometric coefficient 1. Native CPD-627 leaves CoA stereocenters unspecified; the current physiological CoA structure is a narrower representation, not an exact native-structure equivalence.	(3S)-citramalyl-CoA => pyruvate + acetyl-CoA `RHEA:22613`	RHEA:22613 Source assertion: Rhea directed reaction 22613 places CHEBI:58668 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/22613 rh:substrates http://rdf.rhea-db.org/22612_L; http://rdf.rhea-db.org/22612_L rh:contains1 http://rdf.rhea-db.org/Participant_22612_compound_2663; http://rdf.rhea-db.org/Participant_22612_compound_2663 rh:compound http://rdf.rhea-db.org/Compound_2663; rh:accession` MetaCyc:CPD-627 Source assertion: The native S-citramalyl-CoA InChI explicitly leaves five CoA stereocenters unspecified. Current physiological CoA stereochemistry narrows that representation. Source location `Compound[@frameid="CPD-627"]/inchi`
(3S)-citramalyl-CoA => pyruvate + acetyl-CoA `RHEA:22613`	produces Stoichiometric coefficient 1.	pyruvate `CHEBI:15361`	RHEA:22613 Source assertion: Rhea directed reaction 22613 places CHEBI:15361 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/22613 rh:products http://rdf.rhea-db.org/22612_R; http://rdf.rhea-db.org/22612_R rh:contains1 http://rdf.rhea-db.org/Participant_22612_compound_3719; http://rdf.rhea-db.org/Participant_22612_compound_3719 rh:compound http://rdf.rhea-db.org/Compound_3719; rh:accession`
(3S)-citramalyl-CoA => pyruvate + acetyl-CoA `RHEA:22613`	produces Stoichiometric coefficient 1.	acetyl-CoA(4-) `CHEBI:57288`	RHEA:22613 Source assertion: Rhea directed reaction 22613 places CHEBI:57288 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/22613 rh:products http://rdf.rhea-db.org/22612_R; http://rdf.rhea-db.org/22612_R rh:contains1 http://rdf.rhea-db.org/Participant_22612_compound_4271; http://rdf.rhea-db.org/Participant_22612_compound_4271 rh:compound http://rdf.rhea-db.org/Compound_4271; rh:accession`
3-oxopropanoate `CHEBI:33190`	consumes Stoichiometric coefficient 1.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	RHEA:26440 Source assertion: Rhea directed reaction 26440 places CHEBI:33190 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:substrates http://rdf.rhea-db.org/26438_R; http://rdf.rhea-db.org/26438_R rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_5274; http://rdf.rhea-db.org/Participant_26438_compound_5274 rh:compound http://rdf.rhea-db.org/Compound_5274; rh:accession`
NADPH(4-) `CHEBI:57783`	consumes Stoichiometric coefficient 1.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	RHEA:26440 Source assertion: Rhea directed reaction 26440 places CHEBI:57783 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:substrates http://rdf.rhea-db.org/26438_R; http://rdf.rhea-db.org/26438_R rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_3438; http://rdf.rhea-db.org/Participant_26438_compound_3438 rh:compound http://rdf.rhea-db.org/Compound_3438; rh:accession`
hydron `CHEBI:15378`	consumes Stoichiometric coefficient 1.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	RHEA:26440 Source assertion: Rhea directed reaction 26440 places CHEBI:15378 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:substrates http://rdf.rhea-db.org/26438_R; http://rdf.rhea-db.org/26438_R rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_3249; http://rdf.rhea-db.org/Participant_26438_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	produces Stoichiometric coefficient 1.	3-hydroxypropionate `CHEBI:16510`	RHEA:26440 Source assertion: Rhea directed reaction 26440 places CHEBI:16510 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:products http://rdf.rhea-db.org/26438_L; http://rdf.rhea-db.org/26438_L rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_3378; http://rdf.rhea-db.org/Participant_26438_compound_3378 rh:compound http://rdf.rhea-db.org/Compound_3378; rh:accession`
3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	produces Stoichiometric coefficient 1.	NADP(3-) `CHEBI:58349`	RHEA:26440 Source assertion: Rhea directed reaction 26440 places CHEBI:58349 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:products http://rdf.rhea-db.org/26438_L; http://rdf.rhea-db.org/26438_L rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_5188; http://rdf.rhea-db.org/Participant_26438_compound_5188 rh:compound http://rdf.rhea-db.org/Compound_5188; rh:accession`
malonyl-CoA(5-) `CHEBI:57384`	consumes Stoichiometric coefficient 1.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	RHEA:26448 Source assertion: Rhea directed reaction 26448 places CHEBI:57384 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:substrates http://rdf.rhea-db.org/26446_R; http://rdf.rhea-db.org/26446_R rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_5125; http://rdf.rhea-db.org/Participant_26446_compound_5125 rh:compound http://rdf.rhea-db.org/Compound_5125; rh:accession`
NADPH(4-) `CHEBI:57783`	consumes Stoichiometric coefficient 1.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	RHEA:26448 Source assertion: Rhea directed reaction 26448 places CHEBI:57783 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:substrates http://rdf.rhea-db.org/26446_R; http://rdf.rhea-db.org/26446_R rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_3438; http://rdf.rhea-db.org/Participant_26446_compound_3438 rh:compound http://rdf.rhea-db.org/Compound_3438; rh:accession`
hydron `CHEBI:15378`	consumes Stoichiometric coefficient 1.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	RHEA:26448 Source assertion: Rhea directed reaction 26448 places CHEBI:15378 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:substrates http://rdf.rhea-db.org/26446_R; http://rdf.rhea-db.org/26446_R rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_3249; http://rdf.rhea-db.org/Participant_26446_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	produces Stoichiometric coefficient 1.	3-oxopropanoate `CHEBI:33190`	RHEA:26448 Source assertion: Rhea directed reaction 26448 places CHEBI:33190 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:products http://rdf.rhea-db.org/26446_L; http://rdf.rhea-db.org/26446_L rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_5274; http://rdf.rhea-db.org/Participant_26446_compound_5274 rh:compound http://rdf.rhea-db.org/Compound_5274; rh:accession`
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	produces Stoichiometric coefficient 1.	NADP(3-) `CHEBI:58349`	RHEA:26448 Source assertion: Rhea directed reaction 26448 places CHEBI:58349 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:products http://rdf.rhea-db.org/26446_L; http://rdf.rhea-db.org/26446_L rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_5188; http://rdf.rhea-db.org/Participant_26446_compound_5188 rh:compound http://rdf.rhea-db.org/Compound_5188; rh:accession`
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	produces Stoichiometric coefficient 1.	coenzyme A(4-) `CHEBI:57287`	RHEA:26448 Source assertion: Rhea directed reaction 26448 places CHEBI:57287 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:products http://rdf.rhea-db.org/26446_L; http://rdf.rhea-db.org/26446_L rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_1264; http://rdf.rhea-db.org/Participant_26446_compound_1264 rh:compound http://rdf.rhea-db.org/Compound_1264; rh:accession`
acryloyl-CoA(4-) `CHEBI:57367`	consumes Stoichiometric coefficient 1.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	RHEA:26456 Source assertion: Rhea directed reaction 26456 places CHEBI:57367 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:substrates http://rdf.rhea-db.org/26454_R; http://rdf.rhea-db.org/26454_R rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_2006; http://rdf.rhea-db.org/Participant_26454_compound_2006 rh:compound http://rdf.rhea-db.org/Compound_2006; rh:accession`
NADPH(4-) `CHEBI:57783`	consumes Stoichiometric coefficient 1.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	RHEA:26456 Source assertion: Rhea directed reaction 26456 places CHEBI:57783 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:substrates http://rdf.rhea-db.org/26454_R; http://rdf.rhea-db.org/26454_R rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_3438; http://rdf.rhea-db.org/Participant_26454_compound_3438 rh:compound http://rdf.rhea-db.org/Compound_3438; rh:accession`
hydron `CHEBI:15378`	consumes Stoichiometric coefficient 1.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	RHEA:26456 Source assertion: Rhea directed reaction 26456 places CHEBI:15378 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:substrates http://rdf.rhea-db.org/26454_R; http://rdf.rhea-db.org/26454_R rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_3249; http://rdf.rhea-db.org/Participant_26454_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; rh:accession`
acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	produces Stoichiometric coefficient 1.	propionyl-CoA(4-) `CHEBI:57392`	RHEA:26456 Source assertion: Rhea directed reaction 26456 places CHEBI:57392 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:products http://rdf.rhea-db.org/26454_L; http://rdf.rhea-db.org/26454_L rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_3421; http://rdf.rhea-db.org/Participant_26454_compound_3421 rh:compound http://rdf.rhea-db.org/Compound_3421; rh:accession`
acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	produces Stoichiometric coefficient 1.	NADP(3-) `CHEBI:58349`	RHEA:26456 Source assertion: Rhea directed reaction 26456 places CHEBI:58349 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:products http://rdf.rhea-db.org/26454_L; http://rdf.rhea-db.org/26454_L rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_5188; http://rdf.rhea-db.org/Participant_26454_compound_5188 rh:compound http://rdf.rhea-db.org/Compound_5188; rh:accession`
3-hydroxypropanoyl-CoA(4-) `CHEBI:58528`	consumes Stoichiometric coefficient 1.	3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	RHEA:26519 Source assertion: Rhea directed reaction 26519 places CHEBI:58528 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26519 rh:substrates http://rdf.rhea-db.org/26518_L; http://rdf.rhea-db.org/26518_L rh:contains1 http://rdf.rhea-db.org/Participant_26518_compound_6030; http://rdf.rhea-db.org/Participant_26518_compound_6030 rh:compound http://rdf.rhea-db.org/Compound_6030; rh:accession`
3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	produces Stoichiometric coefficient 1.	acryloyl-CoA(4-) `CHEBI:57367`	RHEA:26519 Source assertion: Rhea directed reaction 26519 places CHEBI:57367 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26519 rh:products http://rdf.rhea-db.org/26518_R; http://rdf.rhea-db.org/26518_R rh:contains1 http://rdf.rhea-db.org/Participant_26518_compound_2006; http://rdf.rhea-db.org/Participant_26518_compound_2006 rh:compound http://rdf.rhea-db.org/Compound_2006; rh:accession`
3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	produces Stoichiometric coefficient 1.	water `CHEBI:15377`	RHEA:26519 Source assertion: Rhea directed reaction 26519 places CHEBI:15377 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26519 rh:products http://rdf.rhea-db.org/26518_R; http://rdf.rhea-db.org/26518_R rh:contains1 http://rdf.rhea-db.org/Participant_26518_compound_1283; http://rdf.rhea-db.org/Participant_26518_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; rh:accession`
3-hydroxypropionate `CHEBI:16510`	consumes Stoichiometric coefficient 1.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	RHEA:26535 Source assertion: Rhea directed reaction 26535 places CHEBI:16510 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:substrates http://rdf.rhea-db.org/26534_L; http://rdf.rhea-db.org/26534_L rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_3378; http://rdf.rhea-db.org/Participant_26534_compound_3378 rh:compound http://rdf.rhea-db.org/Compound_3378; rh:accession`
ATP(4-) `CHEBI:30616`	consumes Stoichiometric coefficient 1.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	RHEA:26535 Source assertion: Rhea directed reaction 26535 places CHEBI:30616 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:substrates http://rdf.rhea-db.org/26534_L; http://rdf.rhea-db.org/26534_L rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_6372; http://rdf.rhea-db.org/Participant_26534_compound_6372 rh:compound http://rdf.rhea-db.org/Compound_6372; rh:accession`
coenzyme A(4-) `CHEBI:57287`	consumes Stoichiometric coefficient 1.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	RHEA:26535 Source assertion: Rhea directed reaction 26535 places CHEBI:57287 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:substrates http://rdf.rhea-db.org/26534_L; http://rdf.rhea-db.org/26534_L rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_1264; http://rdf.rhea-db.org/Participant_26534_compound_1264 rh:compound http://rdf.rhea-db.org/Compound_1264; rh:accession`
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	produces Stoichiometric coefficient 1.	3-hydroxypropanoyl-CoA(4-) `CHEBI:58528`	RHEA:26535 Source assertion: Rhea directed reaction 26535 places CHEBI:58528 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:products http://rdf.rhea-db.org/26534_R; http://rdf.rhea-db.org/26534_R rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_6030; http://rdf.rhea-db.org/Participant_26534_compound_6030 rh:compound http://rdf.rhea-db.org/Compound_6030; rh:accession`
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	produces Stoichiometric coefficient 1.	adenosine 5'-monophosphate(2-) `CHEBI:456215`	RHEA:26535 Source assertion: Rhea directed reaction 26535 places CHEBI:456215 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:products http://rdf.rhea-db.org/26534_R; http://rdf.rhea-db.org/26534_R rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_2590; http://rdf.rhea-db.org/Participant_26534_compound_2590 rh:compound http://rdf.rhea-db.org/Compound_2590; rh:accession`
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	produces Stoichiometric coefficient 1.	diphosphate(3-) `CHEBI:33019`	RHEA:26535 Source assertion: Rhea directed reaction 26535 places CHEBI:33019 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:products http://rdf.rhea-db.org/26534_R; http://rdf.rhea-db.org/26534_R rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_2106; http://rdf.rhea-db.org/Participant_26534_compound_2106 rh:compound http://rdf.rhea-db.org/Compound_2106; rh:accession`
propionyl-CoA(4-) `CHEBI:57392`	consumes Stoichiometric coefficient 1.	propanoyl-CoA + glyoxylate => (2R,3S)-beta-methylmalyl-CoA `RHEA:38261`	RHEA:38261 Source assertion: Rhea directed reaction 38261 places CHEBI:57392 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38261 rh:substrates http://rdf.rhea-db.org/38259_R; http://rdf.rhea-db.org/38259_R rh:contains1 http://rdf.rhea-db.org/Participant_38259_compound_3421; http://rdf.rhea-db.org/Participant_38259_compound_3421 rh:compound http://rdf.rhea-db.org/Compound_3421; rh:accession`
glyoxylate `CHEBI:36655`	consumes Stoichiometric coefficient 1.	propanoyl-CoA + glyoxylate => (2R,3S)-beta-methylmalyl-CoA `RHEA:38261`	RHEA:38261 Source assertion: Rhea directed reaction 38261 places CHEBI:36655 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38261 rh:substrates http://rdf.rhea-db.org/38259_R; http://rdf.rhea-db.org/38259_R rh:contains1 http://rdf.rhea-db.org/Participant_38259_compound_3124; http://rdf.rhea-db.org/Participant_38259_compound_3124 rh:compound http://rdf.rhea-db.org/Compound_3124; rh:accession`
propanoyl-CoA + glyoxylate => (2R,3S)-beta-methylmalyl-CoA `RHEA:38261`	produces Stoichiometric coefficient 1. The current acyl stereochemistry agrees with primary Figure 1; native CPD-9409 has the opposite encoded acyl configuration despite its matching label. No exact structure mapping is asserted.	L-erythro-3-methylmalyl-CoA(5-) `CHEBI:75634`	RHEA:38261 Source assertion: Rhea directed reaction 38261 places CHEBI:75634 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38261 rh:products http://rdf.rhea-db.org/38259_L; http://rdf.rhea-db.org/38259_L rh:contains1 http://rdf.rhea-db.org/Participant_38259_compound_8545; http://rdf.rhea-db.org/Participant_38259_compound_8545 rh:compound http://rdf.rhea-db.org/Compound_8545; rh:accession` MetaCyc:CPD-9409 Source assertion: The native compound label is (2R,3S)-beta-methylmalyl-CoA, but its stored InChI encodes the opposite acyl stereocenters from current ChEBI75634. This structured-source conflict is explicitly rejected as an exact mapping. Source location `Compound[@frameid="CPD-9409"]/common-name; inchi` PMID:19955419 Source assertion: Figure 1 explicitly labels the second-cycle condensation product (2R,3S)-beta-methylmalyl-CoA; this is the selected current Rhea structure. Source location `Figure 1, second cycle intermediate between activities 10b and 11`
L-erythro-3-methylmalyl-CoA(5-) `CHEBI:75634`	consumes Stoichiometric coefficient 1. The current acyl stereochemistry agrees with primary Figure 1; native CPD-9409 has the opposite encoded acyl configuration despite its matching label. No exact structure mapping is asserted.	(2R,3S)-beta-methylmalyl-CoA => 2-methylfumaryl-CoA + H2O `RHEA:38264`	RHEA:38264 Source assertion: Rhea directed reaction 38264 places CHEBI:75634 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38264 rh:substrates http://rdf.rhea-db.org/38263_L; http://rdf.rhea-db.org/38263_L rh:contains1 http://rdf.rhea-db.org/Participant_38263_compound_8545; http://rdf.rhea-db.org/Participant_38263_compound_8545 rh:compound http://rdf.rhea-db.org/Compound_8545; rh:accession` MetaCyc:CPD-9409 Source assertion: The native compound label is (2R,3S)-beta-methylmalyl-CoA, but its stored InChI encodes the opposite acyl stereocenters from current ChEBI75634. This structured-source conflict is explicitly rejected as an exact mapping. Source location `Compound[@frameid="CPD-9409"]/common-name; inchi` PMID:19955419 Source assertion: Figure 1 explicitly labels the second-cycle condensation product (2R,3S)-beta-methylmalyl-CoA; this is the selected current Rhea structure. Source location `Figure 1, second cycle intermediate between activities 10b and 11`
(2R,3S)-beta-methylmalyl-CoA => 2-methylfumaryl-CoA + H2O `RHEA:38264`	produces Stoichiometric coefficient 1.	mesaconyl-CoA(5-) `CHEBI:75635`	RHEA:38264 Source assertion: Rhea directed reaction 38264 places CHEBI:75635 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38264 rh:products http://rdf.rhea-db.org/38263_R; http://rdf.rhea-db.org/38263_R rh:contains1 http://rdf.rhea-db.org/Participant_38263_compound_8546; http://rdf.rhea-db.org/Participant_38263_compound_8546 rh:compound http://rdf.rhea-db.org/Compound_8546; rh:accession`
(2R,3S)-beta-methylmalyl-CoA => 2-methylfumaryl-CoA + H2O `RHEA:38264`	produces Stoichiometric coefficient 1.	water `CHEBI:15377`	RHEA:38264 Source assertion: Rhea directed reaction 38264 places CHEBI:15377 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38264 rh:products http://rdf.rhea-db.org/38263_R; http://rdf.rhea-db.org/38263_R rh:contains1 http://rdf.rhea-db.org/Participant_38263_compound_1283; http://rdf.rhea-db.org/Participant_38263_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; rh:accession`
mesaconyl-CoA(5-) `CHEBI:75635`	consumes Stoichiometric coefficient 1.	2-methylfumaryl-CoA => 3-methylfumaryl-CoA `RHEA:38268`	RHEA:38268 Source assertion: Rhea directed reaction 38268 places CHEBI:75635 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38268 rh:substrates http://rdf.rhea-db.org/38267_L; http://rdf.rhea-db.org/38267_L rh:contains1 http://rdf.rhea-db.org/Participant_38267_compound_8546; http://rdf.rhea-db.org/Participant_38267_compound_8546 rh:compound http://rdf.rhea-db.org/Compound_8546; rh:accession`
2-methylfumaryl-CoA => 3-methylfumaryl-CoA `RHEA:38268`	produces Stoichiometric coefficient 1.	3-methylfumaryl-CoA(5-) `CHEBI:75636`	RHEA:38268 Source assertion: Rhea directed reaction 38268 places CHEBI:75636 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38268 rh:products http://rdf.rhea-db.org/38267_R; http://rdf.rhea-db.org/38267_R rh:contains1 http://rdf.rhea-db.org/Participant_38267_compound_8547; http://rdf.rhea-db.org/Participant_38267_compound_8547 rh:compound http://rdf.rhea-db.org/Compound_8547; rh:accession`
3-methylfumaryl-CoA(5-) `CHEBI:75636`	consumes Stoichiometric coefficient 1.	3-methylfumaryl-CoA + H2O => (3S)-citramalyl-CoA `RHEA:38273`	RHEA:38273 Source assertion: Rhea directed reaction 38273 places CHEBI:75636 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38273 rh:substrates http://rdf.rhea-db.org/38271_R; http://rdf.rhea-db.org/38271_R rh:contains1 http://rdf.rhea-db.org/Participant_38271_compound_8547; http://rdf.rhea-db.org/Participant_38271_compound_8547 rh:compound http://rdf.rhea-db.org/Compound_8547; rh:accession`
water `CHEBI:15377`	consumes Stoichiometric coefficient 1.	3-methylfumaryl-CoA + H2O => (3S)-citramalyl-CoA `RHEA:38273`	RHEA:38273 Source assertion: Rhea directed reaction 38273 places CHEBI:15377 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38273 rh:substrates http://rdf.rhea-db.org/38271_R; http://rdf.rhea-db.org/38271_R rh:contains1 http://rdf.rhea-db.org/Participant_38271_compound_1283; http://rdf.rhea-db.org/Participant_38271_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; rh:accession`
3-methylfumaryl-CoA + H2O => (3S)-citramalyl-CoA `RHEA:38273`	produces Stoichiometric coefficient 1. Native CPD-627 leaves CoA stereocenters unspecified; the current physiological CoA structure is a narrower representation, not an exact native-structure equivalence.	(3S)-citramalyl-CoA(5-) `CHEBI:58668`	RHEA:38273 Source assertion: Rhea directed reaction 38273 places CHEBI:58668 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38273 rh:products http://rdf.rhea-db.org/38271_L; http://rdf.rhea-db.org/38271_L rh:contains1 http://rdf.rhea-db.org/Participant_38271_compound_2663; http://rdf.rhea-db.org/Participant_38271_compound_2663 rh:compound http://rdf.rhea-db.org/Compound_2663; rh:accession` MetaCyc:CPD-627 Source assertion: The native S-citramalyl-CoA InChI explicitly leaves five CoA stereocenters unspecified. Current physiological CoA stereochemistry narrows that representation. Source location `Compound[@frameid="CPD-627"]/inchi`
N(6)-carboxybiotinyl-L-lysyl-[protein] `RHEA-COMP:10506`	consumes Stoichiometric coefficient 1.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	RHEA:54729 Source assertion: Rhea directed reaction 54729 places RHEA-COMP:10506 on its substrates side with coefficient 1. This is a whole generic protein-bearing carrier state, not an isolated lysine residue. Source location `http://rdf.rhea-db.org/54729 rh:substrates http://rdf.rhea-db.org/54728_L; http://rdf.rhea-db.org/54728_L rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_10506; http://rdf.rhea-db.org/Participant_54728_compound_10506 rh:compound http://rdf.rhea-db.org/Compound_10506; rh:accession`
acetyl-CoA(4-) `CHEBI:57288`	consumes Stoichiometric coefficient 1.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	RHEA:54729 Source assertion: Rhea directed reaction 54729 places CHEBI:57288 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/54729 rh:substrates http://rdf.rhea-db.org/54728_L; http://rdf.rhea-db.org/54728_L rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_4271; http://rdf.rhea-db.org/Participant_54728_compound_4271 rh:compound http://rdf.rhea-db.org/Compound_4271; rh:accession`
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	produces Stoichiometric coefficient 1.	N(6)-biotinyl-L-lysyl-[protein] `RHEA-COMP:10505`	RHEA:54729 Source assertion: Rhea directed reaction 54729 places RHEA-COMP:10505 on its products side with coefficient 1. This is a whole generic protein-bearing carrier state, not an isolated lysine residue. Source location `http://rdf.rhea-db.org/54729 rh:products http://rdf.rhea-db.org/54728_R; http://rdf.rhea-db.org/54728_R rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_10505; http://rdf.rhea-db.org/Participant_54728_compound_10505 rh:compound http://rdf.rhea-db.org/Compound_10505; rh:accession`
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	produces Stoichiometric coefficient 1.	malonyl-CoA(5-) `CHEBI:57384`	RHEA:54729 Source assertion: Rhea directed reaction 54729 places CHEBI:57384 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/54729 rh:products http://rdf.rhea-db.org/54728_R; http://rdf.rhea-db.org/54728_R rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_5125; http://rdf.rhea-db.org/Participant_54728_compound_5125 rh:compound http://rdf.rhea-db.org/Compound_5125; rh:accession`
`MetaCyc:PWY-5744`	has_part Native CO2/bicarbonate equilibration remains nonenzymatic here; no unidentified carbonic anhydrase is assigned.	CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN0-5224 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN0-5224']` MetaCyc:RXN0-5224 Source assertion: The native reaction links Rhea 10751; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:10750 Source assertion: Rhea master 10748 explicitly links directed member 10750; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/10748 rh:directionalReaction http://rdf.rhea-db.org/10750; http://rdf.rhea-db.org/10750 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists BIOTIN-CARBOXYL-RXN among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='BIOTIN-CARBOXYL-RXN']` MetaCyc:BIOTIN-CARBOXYL-RXN Source assertion: The native reaction links Rhea 13502; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:13502 Source assertion: Rhea master 13501 explicitly links directed member 13502; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/13501 rh:directionalReaction http://rdf.rhea-db.org/13502; http://rdf.rhea-db.org/13502 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN0-5055 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN0-5055']` MetaCyc:RXN0-5055 Source assertion: The native carboxyl-transfer equation transfers a carboxyl group from protein-bound carboxybiotin to acetyl-CoA. Current Rhea retains generic whole protein carrier states. Source location `Reaction[@detail="full"]/left; right` RHEA:54729 Source assertion: Rhea master 54728 explicitly links directed member 54729; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/54728 rh:directionalReaction http://rdf.rhea-db.org/54729; http://rdf.rhea-db.org/54729 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN-8963 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN-8963']` MetaCyc:RXN-8963 Source assertion: The native reaction links Rhea 26449; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26448 Source assertion: Rhea master 26446 explicitly links directed member 26448; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/26446 rh:directionalReaction http://rdf.rhea-db.org/26448; http://rdf.rhea-db.org/26448 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN-8974 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN-8974']` MetaCyc:RXN-8974 Source assertion: The native reaction links Rhea 26440; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26440 Source assertion: Rhea master 26438 explicitly links directed member 26440; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/26438 rh:directionalReaction http://rdf.rhea-db.org/26440; http://rdf.rhea-db.org/26440 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN-9086 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN-9086']` MetaCyc:RXN-9086 Source assertion: The native reaction links Rhea 26535; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26535 Source assertion: Rhea master 26534 explicitly links directed member 26535; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/26534 rh:directionalReaction http://rdf.rhea-db.org/26535; http://rdf.rhea-db.org/26535 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN-6383 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN-6383']` MetaCyc:RXN-6383 Source assertion: The native reaction links Rhea 26518; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26519 Source assertion: Rhea master 26518 explicitly links directed member 26519; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/26518 rh:directionalReaction http://rdf.rhea-db.org/26519; http://rdf.rhea-db.org/26519 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN-9087 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN-9087']` MetaCyc:RXN-9087 Source assertion: The native reaction links Rhea 26456; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:26456 Source assertion: Rhea master 26454 explicitly links directed member 26456; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/26454 rh:directionalReaction http://rdf.rhea-db.org/26456; http://rdf.rhea-db.org/26456 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	propanoyl-CoA + glyoxylate => (2R,3S)-beta-methylmalyl-CoA `RHEA:38261`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN-8961 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN-8961']` MetaCyc:RXN-8961 Source assertion: The native reaction links Rhea 38262; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:38261 Source assertion: Rhea master 38259 explicitly links directed member 38261; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/38259 rh:directionalReaction http://rdf.rhea-db.org/38261; http://rdf.rhea-db.org/38261 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	(2R,3S)-beta-methylmalyl-CoA => 2-methylfumaryl-CoA + H2O `RHEA:38264`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN-8960 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN-8960']` MetaCyc:RXN-8960 Source assertion: The native reaction links Rhea 38266; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:38264 Source assertion: Rhea master 38263 explicitly links directed member 38264; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/38263 rh:directionalReaction http://rdf.rhea-db.org/38264; http://rdf.rhea-db.org/38264 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	2-methylfumaryl-CoA => 3-methylfumaryl-CoA `RHEA:38268`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN-13230 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN-13230']` MetaCyc:RXN-13230 Source assertion: The native reaction links Rhea 38270; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:38268 Source assertion: Rhea master 38267 explicitly links directed member 38268; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/38267 rh:directionalReaction http://rdf.rhea-db.org/38268; http://rdf.rhea-db.org/38268 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	3-methylfumaryl-CoA + H2O => (3S)-citramalyl-CoA `RHEA:38273`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists RXN-8965 among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='RXN-8965']` MetaCyc:RXN-8965 Source assertion: The native equation stores CPD-627 on the left and CPD-14205 plus WATER on the right, with RIGHT-TO-LEFT physiological direction, supporting mesaconyl-C4-CoA hydration to (S)-citramalyl-CoA. Source location `Reaction[@detail="full"]/left; right; reaction-direction` RHEA:38273 Source assertion: Rhea master 38271 explicitly links directed member 38273; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/38271 rh:directionalReaction http://rdf.rhea-db.org/38273; http://rdf.rhea-db.org/38273 rh:substrates; rh:products`
`MetaCyc:PWY-5744`	has_part All native pathway members are retained; normalized current chemical forms are not blanket exact mappings of native structures.	(3S)-citramalyl-CoA => pyruvate + acetyl-CoA `RHEA:22613`	MetaCyc:PWY-5744 Source assertion: PWY-5744 explicitly lists CITRAMALYL-COA-LYASE-RXN among its 13 native reaction objects. Source location `Pathway[@frameid='PWY-5744']/reaction-list/Reaction[@frameid='CITRAMALYL-COA-LYASE-RXN']` MetaCyc:CITRAMALYL-COA-LYASE-RXN Source assertion: The native reaction links Rhea 22615; current family/direction and chemical structure are verified separately. Source location `Reaction[@detail='full']/dblink[dblink-db='RHEA']/dblink-oid` RHEA:22613 Source assertion: Rhea master 22612 explicitly links directed member 22613; the selected sides follow the glyoxylate-assimilation cycle. Source location `http://rdf.rhea-db.org/22612 rh:directionalReaction http://rdf.rhea-db.org/22613; http://rdf.rhea-db.org/22613 rh:substrates; rh:products`
biotin carboxylase `UniProtKB:A9W9X0`	enables J-10-fl BC1 has a fused BCCP domain and a tetrameric biological assembly. This functional relation does not imply monomer sufficiency or fixed whole-ACC stoichiometry.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	PMID:38572988 Source assertion: Caur_1378 BC1 is 596 residues with fused BC/BCCP domains; the biotinylated construct performs biotin-carboxylase/carrier functions, and solution and crystal measurements support a tetramer. Source location `Results s2-1–s2-4; Methods s4-1, s4-3–s4-5, s4-8` UniProtKB:A9W9X0 Source assertion: The current J-10-fl accession is the 596-residue Caur_1378 biotin carboxylase. Evidence tier: ECO:0000256. Source location `/proteinDescription; /genes; /organism; /sequence`
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha `UniProtKB:A9WBQ8`	enables Inferred functional component of the joint alpha/beta carboxyltransferase, not an independently sufficient subunit. The 2024 Methods swaps locus/chain labels, so its exact individual CT construct bridge is not asserted.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	UniProtKB:A9WBQ8 Source assertion: The current HAMAP-backed annotation assigns protein-bound carboxybiotin/acetyl-CoA carboxyltransferase activity Rhea54728. This is an inferred exact-accession function. Evidence tier: ECO:0000256. Source location `/comments/0/texts; /comments/1/reaction; /comments/1/reaction/evidences`
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta `UniProtKB:A9WBQ9`	enables Inferred functional component of the joint alpha/beta carboxyltransferase, not an independently sufficient subunit. The 2024 Methods swaps locus/chain labels, so its exact individual CT construct bridge is not asserted.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	UniProtKB:A9WBQ9 Source assertion: The current HAMAP-backed annotation assigns protein-bound carboxybiotin/acetyl-CoA carboxyltransferase activity Rhea54728. This is an inferred exact-accession function. Evidence tier: ECO:0000255. Source location `/comments/0/texts; /comments/1/reaction; /comments/1/reaction/evidences`
malonyl CoA reductase `MetaCyc:CPLX-7550`	catalyzes Bacterial bifunctional MCR: two NADPH-dependent partial reactions via malonate semialdehyde. The physiological metal is unresolved; metal stimulation does not establish a unique occupied cofactor.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	PMID:11948153 Source assertion: Purified OK-70-fl MCR reduces malonyl-CoA via malonate semialdehyde to 3-hydroxypropionate using two NADPH. Semicarbazide trapping and radiolabel/HPLC support the intermediate and two partial reductions. Source location `Results sec21–sec23; Discussion sec27–sec29` MetaCyc:CPLX-7550 Source assertion: The native bacterial bifunctional MCR complex has two subunits. Source location `Protein[@frameid="CPLX-7550"]/comment; component`
malonyl CoA reductase `MetaCyc:CPLX-7550`	catalyzes Bacterial bifunctional MCR: two NADPH-dependent partial reactions via malonate semialdehyde. The physiological metal is unresolved; metal stimulation does not establish a unique occupied cofactor.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	PMID:11948153 Source assertion: Purified OK-70-fl MCR reduces malonyl-CoA via malonate semialdehyde to 3-hydroxypropionate using two NADPH. Semicarbazide trapping and radiolabel/HPLC support the intermediate and two partial reductions. Source location `Results sec21–sec23; Discussion sec27–sec29` MetaCyc:CPLX-7550 Source assertion: The native bacterial bifunctional MCR complex has two subunits. Source location `Protein[@frameid="CPLX-7550"]/comment; component`
malonyl CoA reductase `MetaCyc:CPLX-7550`	has_part Two equivalent OK-70-fl MCR chains in the experimentally characterized dimer. Native J-10-fl A9WIU3 is a distinct sequence; Q6QQP7 is independently primary-linked, without strain equivalence.	Malonyl-CoA reductase `UniProtKB:Q6QQP7`	PMID:11948153 Source assertion: Purified OK-70-fl MCR had a 145-kDa subunit and about 300-kDa native mass; its reported N-terminal sequence corresponds to the bifunctional SDR-like enzyme. Source location `Methods sec2; Results sec21; Discussion sec29` UniProtKB:Q6QQP7 Source assertion: The sequence record links OK-70-fl, the primary MCR paper and the 2004 sequence submission AY530019/AAS20429.1. Evidence tier: ECO:0000313. Source location `/references/0; /references/1; /uniProtKBCrossReferences[database=EMBL]; /sequence`
acetate--CoA ligase `UniProtKB:Q8VRG6`	enables OK-70-fl trifunctional PCS has a native three-or-four-subunit estimate; no unique complex stoichiometry or monomer sufficiency is asserted. The automated acetate-CoA-ligase label is retained as a label, not adopted as its inspected physiological function.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	MetaCyc:CPLX-7552 Source assertion: The native PCS complex describes all three 3-hydroxypropanoate-to-propanoyl-CoA activities and a native assembly of three or four identical subunits. Source location `Protein[@frameid="CPLX-7552"]/comment; catalyzes` MetaCyc:MONOMER-13612 Source assertion: The native PCS component explicitly cross-references Q8VRG6 as well as the distinct J-10-fl accession A9WEU4. Source location `Protein[@frameid="MONOMER-13612"]/dblink[dblink-db="UNIPROT"]` NCBIProtein:AAL47820.2 Source assertion: The deposited 1822-residue propionyl-CoA synthase derives from strain OK-70-fl and the AF445079 nucleotide record; its annotated product is a three-domain fusion. Evidence tier: ECO:0000313. Source location `GBSeq/GBSeq_accession-version; GBSeq_feature-table; GBSeq_sequence` UniProtKB:Q8VRG6 Source assertion: The primary-linked OK-70-fl PCS sequence cross-references AF445079/AAL47820.2; comparison with the original deposited protein sequence is exact. Evidence tier: ECO:0000313. Source location `/references; /uniProtKBCrossReferences[database=EMBL]; /sequence` PMID:11821399 Source assertion: Purified PCS couples ATP-dependent 3-hydroxypropionate activation to dehydration and NADPH-dependent reduction, yielding propionyl-CoA, AMP and pyrophosphate. Source location `Results: Stoichiometry and Products of the Reaction; Possible Intermediates; Domain Character of Propionyl-CoA Synthase`
acetate--CoA ligase `UniProtKB:Q8VRG6`	enables OK-70-fl trifunctional PCS has a native three-or-four-subunit estimate; no unique complex stoichiometry or monomer sufficiency is asserted. The automated acetate-CoA-ligase label is retained as a label, not adopted as its inspected physiological function.	3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	MetaCyc:CPLX-7552 Source assertion: The native PCS complex describes all three 3-hydroxypropanoate-to-propanoyl-CoA activities and a native assembly of three or four identical subunits. Source location `Protein[@frameid="CPLX-7552"]/comment; catalyzes` MetaCyc:MONOMER-13612 Source assertion: The native PCS component explicitly cross-references Q8VRG6 as well as the distinct J-10-fl accession A9WEU4. Source location `Protein[@frameid="MONOMER-13612"]/dblink[dblink-db="UNIPROT"]` NCBIProtein:AAL47820.2 Source assertion: The deposited 1822-residue propionyl-CoA synthase derives from strain OK-70-fl and the AF445079 nucleotide record; its annotated product is a three-domain fusion. Evidence tier: ECO:0000313. Source location `GBSeq/GBSeq_accession-version; GBSeq_feature-table; GBSeq_sequence` UniProtKB:Q8VRG6 Source assertion: The primary-linked OK-70-fl PCS sequence cross-references AF445079/AAL47820.2; comparison with the original deposited protein sequence is exact. Evidence tier: ECO:0000313. Source location `/references; /uniProtKBCrossReferences[database=EMBL]; /sequence` PMID:11821399 Source assertion: Purified PCS couples ATP-dependent 3-hydroxypropionate activation to dehydration and NADPH-dependent reduction, yielding propionyl-CoA, AMP and pyrophosphate. Source location `Results: Stoichiometry and Products of the Reaction; Possible Intermediates; Domain Character of Propionyl-CoA Synthase`
acetate--CoA ligase `UniProtKB:Q8VRG6`	enables OK-70-fl trifunctional PCS has a native three-or-four-subunit estimate; no unique complex stoichiometry or monomer sufficiency is asserted. The automated acetate-CoA-ligase label is retained as a label, not adopted as its inspected physiological function.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	MetaCyc:CPLX-7552 Source assertion: The native PCS complex describes all three 3-hydroxypropanoate-to-propanoyl-CoA activities and a native assembly of three or four identical subunits. Source location `Protein[@frameid="CPLX-7552"]/comment; catalyzes` MetaCyc:MONOMER-13612 Source assertion: The native PCS component explicitly cross-references Q8VRG6 as well as the distinct J-10-fl accession A9WEU4. Source location `Protein[@frameid="MONOMER-13612"]/dblink[dblink-db="UNIPROT"]` NCBIProtein:AAL47820.2 Source assertion: The deposited 1822-residue propionyl-CoA synthase derives from strain OK-70-fl and the AF445079 nucleotide record; its annotated product is a three-domain fusion. Evidence tier: ECO:0000313. Source location `GBSeq/GBSeq_accession-version; GBSeq_feature-table; GBSeq_sequence` UniProtKB:Q8VRG6 Source assertion: The primary-linked OK-70-fl PCS sequence cross-references AF445079/AAL47820.2; comparison with the original deposited protein sequence is exact. Evidence tier: ECO:0000313. Source location `/references; /uniProtKBCrossReferences[database=EMBL]; /sequence` PMID:11821399 Source assertion: Purified PCS couples ATP-dependent 3-hydroxypropionate activation to dehydration and NADPH-dependent reduction, yielding propionyl-CoA, AMP and pyrophosphate. Source location `Results: Stoichiometry and Products of the Reaction; Possible Intermediates; Domain Character of Propionyl-CoA Synthase`
L-malyl CoA lyase `MetaCyc:CPLX-7549`	catalyzes The same hexameric Mcl catalyzes both glyoxylate/propionyl-CoA condensation and S-citramalyl-CoA cleavage; R-citramalyl-CoA is excluded by the primary assay.	propanoyl-CoA + glyoxylate => (2R,3S)-beta-methylmalyl-CoA `RHEA:38261`	UniProtKB:S5N020 Source assertion: Reviewed OK-70-fl Mcl assigns the selected beta-methylmalyl/citramalyl reaction with experimental evidence. Source location `/comments/0/texts; /comments/2/reaction` PMID:19955419 Source assertion: Recombinant MMC lyase catalyzed propionyl-CoA condensation with glyoxylate and S-citramalyl-CoA cleavage. R-malyl-CoA and R-citramalyl-CoA were not substrates. Source location `Results sec6; Figure 4`
L-malyl CoA lyase `MetaCyc:CPLX-7549`	catalyzes The same hexameric Mcl catalyzes both glyoxylate/propionyl-CoA condensation and S-citramalyl-CoA cleavage; R-citramalyl-CoA is excluded by the primary assay.	(3S)-citramalyl-CoA => pyruvate + acetyl-CoA `RHEA:22613`	UniProtKB:S5N020 Source assertion: Reviewed OK-70-fl Mcl assigns the selected beta-methylmalyl/citramalyl reaction with experimental evidence. Source location `/comments/0/texts; /comments/3/reaction` PMID:19955419 Source assertion: Recombinant MMC lyase catalyzed propionyl-CoA condensation with glyoxylate and S-citramalyl-CoA cleavage. R-malyl-CoA and R-citramalyl-CoA were not substrates. Source location `Results sec6; Figure 4`
L-malyl CoA lyase `MetaCyc:CPLX-7549`	has_part Six identical Mcl chains. Exact OK-70-fl S5N020 and native-mapped J-10-fl A9WC35 sequences are identical, but organism strain identities are not merged.	Malyl-CoA/beta-methylmalyl-CoA/citramalyl-CoA lyase `UniProtKB:S5N020`	MetaCyc:CPLX-7549 Source assertion: The native Mcl assembly lists six copies of MONOMER-13604. Source location `Protein[@frameid="CPLX-7549"]/component` MetaCyc:MONOMER-13604 Source assertion: The native Mcl component explicitly links A9WC35. Source location `Protein[@frameid="MONOMER-13604"]/dblink[dblink-db="UNIPROT"]` PMID:24206647 Source assertion: OK-70-fl Mcl AGR55786 (Table 1) had solution mass 228 +/- 15 kDa and a crystal homohexamer, organized as a dimer of trimers. Source location `Table 1; Results sec6, sec9; Methods sec18` UniProtKB:S5N020 Source assertion: The reviewed OK-70-fl sequence links AGR55786.1/KF240561 and annotates the experimental homohexamer. Source location `/references; /uniProtKBCrossReferences[database=EMBL]; /sequence; /comments/7/texts`
beta-methylmalyl-CoA dehydratase `MetaCyc:CPLX-8468`	catalyzes Native species-level dimer with reviewed current component attribution. Original pathway experiments used OK-70-fl and J-10-fl-guided primers; exact clone/genome equivalence is not presumed.	(2R,3S)-beta-methylmalyl-CoA => 2-methylfumaryl-CoA + H2O `RHEA:38264`	PMID:18065535 Source assertion: Recombinant C. aurantiacus Mch reversibly dehydrates erythro-beta-methylmalyl-CoA to mesaconyl-C1-CoA; HPLC/MS/NMR characterize the product. Source location `Results sec28–sec37; Discussion sec38` UniProtKB:A9WC34 Source assertion: The current reviewed entry assigns this exact reaction family with experimental evidence; the primary functional reference identifies OK-70-fl while the genome-sequence reference identifies J-10-fl. Source location `/comments/0/texts; /comments/1/reaction; /references/0; /references/1`
beta-methylmalyl-CoA dehydratase `MetaCyc:CPLX-8468`	has_part Two copies of the native-mapped current component; the original biochemical construct and current genome strain remain explicitly distinguished.	Beta-methylmalyl-CoA dehydratase `UniProtKB:A9WC34`	MetaCyc:CPLX-8468 Source assertion: The native complex contains two copies of MONOMER-17292. Source location `Protein[@frameid="CPLX-8468"]/component` MetaCyc:MONOMER-17292 Source assertion: The native monomer cross-references UniProt A9WC34. Source location `Protein[@frameid="MONOMER-17292"]/dblink[dblink-db="UNIPROT"]` UniProtKB:A9WC34 Source assertion: The current subunit annotation is homodimer with experimental evidence. Source location `/comments/3/texts`
mesaconyl-C1-CoA-C4-CoA transferase `MetaCyc:CPLX-8466`	catalyzes Native species-level dimer with reviewed current component attribution. Original pathway experiments used OK-70-fl and J-10-fl-guided primers; exact clone/genome equivalence is not presumed.	2-methylfumaryl-CoA => 3-methylfumaryl-CoA `RHEA:38268`	PMID:19955419 Source assertion: Recombinant Mct reversibly converts mesaconyl-C1-CoA to mesaconyl-C4-CoA; NMR distinguishes the positional CoA isomers. Gel filtration supports a homodimer. Source location `Results sec3–sec4` UniProtKB:A9WC36 Source assertion: The current reviewed entry assigns this exact reaction family with experimental evidence; the primary functional reference identifies OK-70-fl while the genome-sequence reference identifies J-10-fl. Source location `/comments/0/texts; /comments/1/reaction; /references/0; /references/1` PMID:36535006 Source assertion: The later study explicitly names A9WC36, reuses pMCTCa_JZ05 and demonstrates the intramolecular isomerase mechanism in an intertwined homodimer. Slow intermolecular side reactions do not redefine the pathway reaction. Source location `Accession Codes (notes-3); Materials and Methods sec2.2; Results sec3.1–sec3.3`
mesaconyl-C1-CoA-C4-CoA transferase `MetaCyc:CPLX-8466`	has_part Two copies of the native-mapped current component; the original biochemical construct and current genome strain remain explicitly distinguished.	2-methylfumaryl-CoA isomerase `UniProtKB:A9WC36`	MetaCyc:CPLX-8466 Source assertion: The native complex contains two copies of MONOMER-17293. Source location `Protein[@frameid="CPLX-8466"]/component` MetaCyc:MONOMER-17293 Source assertion: The native monomer cross-references UniProt A9WC36. Source location `Protein[@frameid="MONOMER-17293"]/dblink[dblink-db="UNIPROT"]` UniProtKB:A9WC36 Source assertion: The current subunit annotation is homodimer with experimental evidence. Source location `/comments/4/texts`
mesaconyl-C4-CoA hydratase `MetaCyc:CPLX-8467`	catalyzes Native species-level dimer with reviewed current component attribution. Original pathway experiments used OK-70-fl and J-10-fl-guided primers; exact clone/genome equivalence is not presumed.	3-methylfumaryl-CoA + H2O => (3S)-citramalyl-CoA `RHEA:38273`	PMID:19955419 Source assertion: Purified native and recombinant Meh hydrate mesaconyl-C4-CoA to S-citramalyl-CoA. The coupled sequence yields acetyl-CoA and pyruvate; gel filtration supports a homodimer. Source location `Results sec5–sec6; Figure 4` UniProtKB:A9WC41 Source assertion: The current reviewed entry assigns this exact reaction family with experimental evidence; the primary functional reference identifies OK-70-fl while the genome-sequence reference identifies J-10-fl. Source location `/comments/0/texts; /comments/1/reaction; /references/0; /references/1`
mesaconyl-C4-CoA hydratase `MetaCyc:CPLX-8467`	has_part Two copies of the native-mapped current component; the original biochemical construct and current genome strain remain explicitly distinguished.	Mesaconyl-C(4)-CoA hydratase `UniProtKB:A9WC41`	MetaCyc:CPLX-8467 Source assertion: The native complex contains two copies of MONOMER-17294. Source location `Protein[@frameid="CPLX-8467"]/component` MetaCyc:MONOMER-17294 Source assertion: The native monomer cross-references UniProt A9WC41. Source location `Protein[@frameid="MONOMER-17294"]/dblink[dblink-db="UNIPROT"]` UniProtKB:A9WC41 Source assertion: The current subunit annotation is homodimer with experimental evidence. Source location `/comments/4/texts`
biotin carboxylase `UniProtKB:A9W9X0`	has_cofactor The source evidence tier is preserved.	biotinate `CHEBI:57586`	UniProtKB:A9W9X0 Source assertion: The current protein annotation assigns biotin as cofactor by ARBA; the catalytic graph separately retains the covalently attached carrier states. Evidence tier: ECO:0000256. Source location `/comments/0/cofactors`
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta `UniProtKB:A9WBQ9`	has_cofactor The source evidence tier is preserved.	zinc(2+) `CHEBI:29105`	UniProtKB:A9WBQ9 Source assertion: The current beta-chain annotation assigns one zinc per subunit by HAMAP; this is not a direct experimental J-10-fl metal-occupancy measurement. Evidence tier: ECO:0000255. Source location `/comments/2/cofactors; /comments/2/note`
L-malyl CoA lyase `MetaCyc:CPLX-7549`	has_cofactor Alternative divalent-metal support, not simultaneous obligatory binding of both ions.	magnesium(2+) `CHEBI:18420`	UniProtKB:S5N020 Source assertion: The reviewed Mcl cofactor annotation includes magnesium, with experimental evidence and an explicit divalent-cation qualification. Source location `/comments/4/cofactors; /comments/4/note`
L-malyl CoA lyase `MetaCyc:CPLX-7549`	has_cofactor Alternative divalent-metal support, not simultaneous obligatory binding of both ions.	manganese(2+) `CHEBI:29035`	UniProtKB:S5N020 Source assertion: The reviewed Mcl cofactor annotation includes manganese, with experimental evidence and an explicit divalent-cation qualification. Source location `/comments/4/cofactors; /comments/4/note`
acetate--CoA ligase `UniProtKB:Q8VRG6`	has_cofactor PCS requires a divalent cation in the inspected assay; magnesium is the selected assay cofactor, not an inferred exact occupancy count.	magnesium(2+) `CHEBI:18420`	PMID:11821399 Source assertion: PCS activity required Mg2+ and was stimulated by K+; the reduction uses NADPH rather than NADH. Source location `Results: Reductive Conversion of 3-Hydroxypropionate by Cell Extracts; Catalytic Properties of Propionyl-CoA Synthase`
acetate--CoA ligase `UniProtKB:Q8VRG6`	has_cofactor Potassium-dependent PCS ligase activity in the inspected assay; partial Rb+, NH4+ and Cs+ substitution is retained as a condition, not a unique in-vivo occupancy assignment.	potassium(1+) `CHEBI:29103`	PMID:11821399 Source assertion: PCS activity depended on potassium, with half-maximal activity at 4 mM KCl; related monovalent cations could partly substitute. The authors attribute this dependence to the CoA-ligase step. Source location `Results: Catalytic Properties of Propionyl-CoA Synthase`
hydroxylamine `CHEBI:15429`	inhibits Partial inactivation in the substrate-present assay; not a physiological regulatory mechanism.	mesaconyl-C1-CoA-C4-CoA transferase `MetaCyc:CPLX-8466`	PMID:19955419 Source assertion: Hydroxylamine left about 60% Mct activity under the tested inactivation conditions; borohydride had no observed effect. Source location `Results sec4, final paragraph`
3-methylfumaryl-CoA(5-) `CHEBI:75636`	inhibits Substrate inhibition only above the measured concentration threshold; this does not contradict its consumption as substrate.	mesaconyl-C4-CoA hydratase `MetaCyc:CPLX-8467`	PMID:19955419 Source assertion: The recombinant Meh hydratase was inhibited by mesaconyl-C4-CoA concentrations above 0.3 mM. Source location `Results sec5, paragraph following Figure 4`
CO2 + H2O => hydrogencarbonate + H(+) `RHEA:10750`	provides_input_for Material continuity through CHEBI:17544; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:10750 Source assertion: Rhea directed reaction 10750 places CHEBI:17544 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/10750 rh:products http://rdf.rhea-db.org/10748_L; http://rdf.rhea-db.org/10748_L rh:contains1 http://rdf.rhea-db.org/Participant_10748_compound_1123; http://rdf.rhea-db.org/Participant_10748_compound_1123 rh:compound http://rdf.rhea-db.org/Compound_1123; rh:accession` RHEA:13502 Source assertion: Rhea directed reaction 13502 places CHEBI:17544 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/13502 rh:substrates http://rdf.rhea-db.org/13501_L; http://rdf.rhea-db.org/13501_L rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_1123; http://rdf.rhea-db.org/Participant_13501_compound_1123 rh:compound http://rdf.rhea-db.org/Compound_1123; rh:accession`
N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	provides_input_for Material continuity through RHEA-COMP:10506; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	RHEA:13502 Source assertion: Rhea directed reaction 13502 places RHEA-COMP:10506 on its products side with coefficient 1. This is a whole generic protein-bearing carrier state, not an isolated lysine residue. Source location `http://rdf.rhea-db.org/13502 rh:products http://rdf.rhea-db.org/13501_R; http://rdf.rhea-db.org/13501_R rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_10506; http://rdf.rhea-db.org/Participant_13501_compound_10506 rh:compound http://rdf.rhea-db.org/Compound_10506; rh:accession` RHEA:54729 Source assertion: Rhea directed reaction 54729 places RHEA-COMP:10506 on its substrates side with coefficient 1. This is a whole generic protein-bearing carrier state, not an isolated lysine residue. Source location `http://rdf.rhea-db.org/54729 rh:substrates http://rdf.rhea-db.org/54728_L; http://rdf.rhea-db.org/54728_L rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_10506; http://rdf.rhea-db.org/Participant_54728_compound_10506 rh:compound http://rdf.rhea-db.org/Compound_10506; rh:accession`
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	provides_input_for Material continuity through RHEA-COMP:10505; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) `RHEA:13502`	RHEA:54729 Source assertion: Rhea directed reaction 54729 places RHEA-COMP:10505 on its products side with coefficient 1. This is a whole generic protein-bearing carrier state, not an isolated lysine residue. Source location `http://rdf.rhea-db.org/54729 rh:products http://rdf.rhea-db.org/54728_R; http://rdf.rhea-db.org/54728_R rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_10505; http://rdf.rhea-db.org/Participant_54728_compound_10505 rh:compound http://rdf.rhea-db.org/Compound_10505; rh:accession` RHEA:13502 Source assertion: Rhea directed reaction 13502 places RHEA-COMP:10505 on its substrates side with coefficient 1. This is a whole generic protein-bearing carrier state, not an isolated lysine residue. Source location `http://rdf.rhea-db.org/13502 rh:substrates http://rdf.rhea-db.org/13501_L; http://rdf.rhea-db.org/13501_L rh:contains1 http://rdf.rhea-db.org/Participant_13501_compound_10505; http://rdf.rhea-db.org/Participant_13501_compound_10505 rh:compound http://rdf.rhea-db.org/Compound_10505; rh:accession`
N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	provides_input_for Material continuity through CHEBI:57384; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	RHEA:54729 Source assertion: Rhea directed reaction 54729 places CHEBI:57384 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/54729 rh:products http://rdf.rhea-db.org/54728_R; http://rdf.rhea-db.org/54728_R rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_5125; http://rdf.rhea-db.org/Participant_54728_compound_5125 rh:compound http://rdf.rhea-db.org/Compound_5125; rh:accession` RHEA:26448 Source assertion: Rhea directed reaction 26448 places CHEBI:57384 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:substrates http://rdf.rhea-db.org/26446_R; http://rdf.rhea-db.org/26446_R rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_5125; http://rdf.rhea-db.org/Participant_26446_compound_5125 rh:compound http://rdf.rhea-db.org/Compound_5125; rh:accession`
malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA `RHEA:26448`	provides_input_for Material continuity through CHEBI:33190; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	RHEA:26448 Source assertion: Rhea directed reaction 26448 places CHEBI:33190 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26448 rh:products http://rdf.rhea-db.org/26446_L; http://rdf.rhea-db.org/26446_L rh:contains1 http://rdf.rhea-db.org/Participant_26446_compound_5274; http://rdf.rhea-db.org/Participant_26446_compound_5274 rh:compound http://rdf.rhea-db.org/Compound_5274; rh:accession` RHEA:26440 Source assertion: Rhea directed reaction 26440 places CHEBI:33190 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:substrates http://rdf.rhea-db.org/26438_R; http://rdf.rhea-db.org/26438_R rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_5274; http://rdf.rhea-db.org/Participant_26438_compound_5274 rh:compound http://rdf.rhea-db.org/Compound_5274; rh:accession`
3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) `RHEA:26440`	provides_input_for Material continuity through CHEBI:16510; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	RHEA:26440 Source assertion: Rhea directed reaction 26440 places CHEBI:16510 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26440 rh:products http://rdf.rhea-db.org/26438_L; http://rdf.rhea-db.org/26438_L rh:contains1 http://rdf.rhea-db.org/Participant_26438_compound_3378; http://rdf.rhea-db.org/Participant_26438_compound_3378 rh:compound http://rdf.rhea-db.org/Compound_3378; rh:accession` RHEA:26535 Source assertion: Rhea directed reaction 26535 places CHEBI:16510 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:substrates http://rdf.rhea-db.org/26534_L; http://rdf.rhea-db.org/26534_L rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_3378; http://rdf.rhea-db.org/Participant_26534_compound_3378 rh:compound http://rdf.rhea-db.org/Compound_3378; rh:accession`
3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate `RHEA:26535`	provides_input_for Material continuity through CHEBI:58528; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	RHEA:26535 Source assertion: Rhea directed reaction 26535 places CHEBI:58528 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26535 rh:products http://rdf.rhea-db.org/26534_R; http://rdf.rhea-db.org/26534_R rh:contains1 http://rdf.rhea-db.org/Participant_26534_compound_6030; http://rdf.rhea-db.org/Participant_26534_compound_6030 rh:compound http://rdf.rhea-db.org/Compound_6030; rh:accession` RHEA:26519 Source assertion: Rhea directed reaction 26519 places CHEBI:58528 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26519 rh:substrates http://rdf.rhea-db.org/26518_L; http://rdf.rhea-db.org/26518_L rh:contains1 http://rdf.rhea-db.org/Participant_26518_compound_6030; http://rdf.rhea-db.org/Participant_26518_compound_6030 rh:compound http://rdf.rhea-db.org/Compound_6030; rh:accession`
3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O `RHEA:26519`	provides_input_for Material continuity through CHEBI:57367; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	RHEA:26519 Source assertion: Rhea directed reaction 26519 places CHEBI:57367 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26519 rh:products http://rdf.rhea-db.org/26518_R; http://rdf.rhea-db.org/26518_R rh:contains1 http://rdf.rhea-db.org/Participant_26518_compound_2006; http://rdf.rhea-db.org/Participant_26518_compound_2006 rh:compound http://rdf.rhea-db.org/Compound_2006; rh:accession` RHEA:26456 Source assertion: Rhea directed reaction 26456 places CHEBI:57367 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:substrates http://rdf.rhea-db.org/26454_R; http://rdf.rhea-db.org/26454_R rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_2006; http://rdf.rhea-db.org/Participant_26454_compound_2006 rh:compound http://rdf.rhea-db.org/Compound_2006; rh:accession`
acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) `RHEA:26456`	provides_input_for Material continuity through CHEBI:57392; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	propanoyl-CoA + glyoxylate => (2R,3S)-beta-methylmalyl-CoA `RHEA:38261`	RHEA:26456 Source assertion: Rhea directed reaction 26456 places CHEBI:57392 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/26456 rh:products http://rdf.rhea-db.org/26454_L; http://rdf.rhea-db.org/26454_L rh:contains1 http://rdf.rhea-db.org/Participant_26454_compound_3421; http://rdf.rhea-db.org/Participant_26454_compound_3421 rh:compound http://rdf.rhea-db.org/Compound_3421; rh:accession` RHEA:38261 Source assertion: Rhea directed reaction 38261 places CHEBI:57392 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38261 rh:substrates http://rdf.rhea-db.org/38259_R; http://rdf.rhea-db.org/38259_R rh:contains1 http://rdf.rhea-db.org/Participant_38259_compound_3421; http://rdf.rhea-db.org/Participant_38259_compound_3421 rh:compound http://rdf.rhea-db.org/Compound_3421; rh:accession`
propanoyl-CoA + glyoxylate => (2R,3S)-beta-methylmalyl-CoA `RHEA:38261`	provides_input_for Material continuity through CHEBI:75634; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	(2R,3S)-beta-methylmalyl-CoA => 2-methylfumaryl-CoA + H2O `RHEA:38264`	RHEA:38261 Source assertion: Rhea directed reaction 38261 places CHEBI:75634 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38261 rh:products http://rdf.rhea-db.org/38259_L; http://rdf.rhea-db.org/38259_L rh:contains1 http://rdf.rhea-db.org/Participant_38259_compound_8545; http://rdf.rhea-db.org/Participant_38259_compound_8545 rh:compound http://rdf.rhea-db.org/Compound_8545; rh:accession` RHEA:38264 Source assertion: Rhea directed reaction 38264 places CHEBI:75634 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38264 rh:substrates http://rdf.rhea-db.org/38263_L; http://rdf.rhea-db.org/38263_L rh:contains1 http://rdf.rhea-db.org/Participant_38263_compound_8545; http://rdf.rhea-db.org/Participant_38263_compound_8545 rh:compound http://rdf.rhea-db.org/Compound_8545; rh:accession`
(2R,3S)-beta-methylmalyl-CoA => 2-methylfumaryl-CoA + H2O `RHEA:38264`	provides_input_for Material continuity through CHEBI:75635; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	2-methylfumaryl-CoA => 3-methylfumaryl-CoA `RHEA:38268`	RHEA:38264 Source assertion: Rhea directed reaction 38264 places CHEBI:75635 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38264 rh:products http://rdf.rhea-db.org/38263_R; http://rdf.rhea-db.org/38263_R rh:contains1 http://rdf.rhea-db.org/Participant_38263_compound_8546; http://rdf.rhea-db.org/Participant_38263_compound_8546 rh:compound http://rdf.rhea-db.org/Compound_8546; rh:accession` RHEA:38268 Source assertion: Rhea directed reaction 38268 places CHEBI:75635 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38268 rh:substrates http://rdf.rhea-db.org/38267_L; http://rdf.rhea-db.org/38267_L rh:contains1 http://rdf.rhea-db.org/Participant_38267_compound_8546; http://rdf.rhea-db.org/Participant_38267_compound_8546 rh:compound http://rdf.rhea-db.org/Compound_8546; rh:accession`
2-methylfumaryl-CoA => 3-methylfumaryl-CoA `RHEA:38268`	provides_input_for Material continuity through CHEBI:75636; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	3-methylfumaryl-CoA + H2O => (3S)-citramalyl-CoA `RHEA:38273`	RHEA:38268 Source assertion: Rhea directed reaction 38268 places CHEBI:75636 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38268 rh:products http://rdf.rhea-db.org/38267_R; http://rdf.rhea-db.org/38267_R rh:contains1 http://rdf.rhea-db.org/Participant_38267_compound_8547; http://rdf.rhea-db.org/Participant_38267_compound_8547 rh:compound http://rdf.rhea-db.org/Compound_8547; rh:accession` RHEA:38273 Source assertion: Rhea directed reaction 38273 places CHEBI:75636 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/38273 rh:substrates http://rdf.rhea-db.org/38271_R; http://rdf.rhea-db.org/38271_R rh:contains1 http://rdf.rhea-db.org/Participant_38271_compound_8547; http://rdf.rhea-db.org/Participant_38271_compound_8547 rh:compound http://rdf.rhea-db.org/Compound_8547; rh:accession`
3-methylfumaryl-CoA + H2O => (3S)-citramalyl-CoA `RHEA:38273`	provides_input_for Material continuity through CHEBI:58668; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	(3S)-citramalyl-CoA => pyruvate + acetyl-CoA `RHEA:22613`	RHEA:38273 Source assertion: Rhea directed reaction 38273 places CHEBI:58668 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/38273 rh:products http://rdf.rhea-db.org/38271_L; http://rdf.rhea-db.org/38271_L rh:contains1 http://rdf.rhea-db.org/Participant_38271_compound_2663; http://rdf.rhea-db.org/Participant_38271_compound_2663 rh:compound http://rdf.rhea-db.org/Compound_2663; rh:accession` RHEA:22613 Source assertion: Rhea directed reaction 22613 places CHEBI:58668 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/22613 rh:substrates http://rdf.rhea-db.org/22612_L; http://rdf.rhea-db.org/22612_L rh:contains1 http://rdf.rhea-db.org/Participant_22612_compound_2663; http://rdf.rhea-db.org/Participant_22612_compound_2663 rh:compound http://rdf.rhea-db.org/Compound_2663; rh:accession`
(3S)-citramalyl-CoA => pyruvate + acetyl-CoA `RHEA:22613`	provides_input_for Material continuity through CHEBI:57288; this is a cycle, not evidence of obligatory channeling or irreversible kinetic order.	N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA `RHEA:54729`	RHEA:22613 Source assertion: Rhea directed reaction 22613 places CHEBI:57288 on its products side with coefficient 1. Source location `http://rdf.rhea-db.org/22613 rh:products http://rdf.rhea-db.org/22612_R; http://rdf.rhea-db.org/22612_R rh:contains1 http://rdf.rhea-db.org/Participant_22612_compound_4271; http://rdf.rhea-db.org/Participant_22612_compound_4271 rh:compound http://rdf.rhea-db.org/Compound_4271; rh:accession` RHEA:54729 Source assertion: Rhea directed reaction 54729 places CHEBI:57288 on its substrates side with coefficient 1. Source location `http://rdf.rhea-db.org/54729 rh:substrates http://rdf.rhea-db.org/54728_L; http://rdf.rhea-db.org/54728_L rh:contains1 http://rdf.rhea-db.org/Participant_54728_compound_4271; http://rdf.rhea-db.org/Participant_54728_compound_4271 rh:compound http://rdf.rhea-db.org/Compound_4271; rh:accession`

References

MetaCyc:PWY-5744 glyoxylate assimilation (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
PMID:19955419 Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus (source version: Complete original primary full-text body retrieved 2026-10-11; strain and construct limits retained)
PMID:11948153 Malonyl-Coenzyme A Reductase from Chloroflexus aurantiacus, a Key Enzyme of the 3-Hydroxypropionate Cycle for Autotrophic CO2 Fixation (source version: Complete original primary full-text body retrieved 2026-10-11; strain and construct limits retained)
PMID:38572988 Chloroflexus aurantiacus acetyl-CoA carboxylase evolves fused biotin carboxylase and biotin carboxyl carrier protein to complete carboxylation activity (source version: Complete original primary full-text body retrieved 2026-10-11; strain and construct limits retained)
PMID:24206647 The crystal structures of the tri-functional Chloroflexus aurantiacus and bi-functional Rhodobacter sphaeroides malyl-CoA lyases and comparison with CitE-like superfamily enzymes and malate synthases (source version: Complete original primary full-text body retrieved 2026-10-11; strain and construct limits retained)
PMID:18065535 Mesaconyl-Coenzyme A Hydratase, a New Enzyme of Two Central Carbon Metabolic Pathways in Bacteria (source version: Complete original primary full-text body retrieved 2026-10-11; strain and construct limits retained)
PMID:12374834 l-Malyl-Coenzyme A Lyase/β-Methylmalyl-Coenzyme A Lyase from Chloroflexus aurantiacus, a Bifunctional Enzyme Involved in Autotrophic CO2 Fixation (source version: Complete original primary full-text body retrieved 2026-10-11; strain and construct limits retained)
PMID:36535006 Structural Basis for a Cork-Up Mechanism of the Intra-Molecular Mesaconyl-CoA Transferase (source version: Complete original primary full-text body retrieved 2026-10-11; strain and construct limits retained)
PMID:11821399 Propionyl-coenzyme A synthase from Chloroflexus aurantiacus, a key enzyme of the 3-hydroxypropionate cycle for autotrophic CO2 fixation (source version: Primary full-text copy inspected through web rendering 2026-10-11; local publisher and author-copy downloads returned 403; no body digest or local full-text replay claimed)
NCBIProtein:AAL47820.2 Propionyl-CoA synthase [Chloroflexus aurantiacus] (source version: Complete original accession-version protein XML retrieved 2026-10-11)
RHEA:10750 CO2 + H2O => hydrogencarbonate + H(+) (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN0-5224 RXN0-5224 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:13502 N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP => N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:BIOTIN-CARBOXYL-RXN BIOTIN-CARBOXYL-RXN (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:54729 N(6)-carboxybiotinyl-L-lysyl-[protein] + acetyl-CoA => N(6)-biotinyl-L-lysyl-[protein] + malonyl-CoA (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN0-5055 RXN0-5055 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:26448 malonyl-CoA + NADPH + H(+) => 3-oxopropanoate + NADP(+) + CoA (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN-8963 RXN-8963 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:26440 3-oxopropanoate + NADPH + H(+) => 3-hydroxypropanoate + NADP(+) (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN-8974 RXN-8974 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:26535 3-hydroxypropanoate + ATP + CoA => 3-hydroxypropanoyl-CoA + AMP + diphosphate (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN-9086 RXN-9086 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:26519 3-hydroxypropanoyl-CoA => acryloyl-CoA + H2O (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN-6383 RXN-6383 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:26456 acryloyl-CoA + NADPH + H(+) => propanoyl-CoA + NADP(+) (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN-9087 RXN-9087 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:38261 propanoyl-CoA + glyoxylate => (2R,3S)-beta-methylmalyl-CoA (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN-8961 RXN-8961 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:38264 (2R,3S)-beta-methylmalyl-CoA => 2-methylfumaryl-CoA + H2O (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN-8960 RXN-8960 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:38268 2-methylfumaryl-CoA => 3-methylfumaryl-CoA (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN-13230 RXN-13230 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:38273 3-methylfumaryl-CoA + H2O => (3S)-citramalyl-CoA (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:RXN-8965 RXN-8965 (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
RHEA:22613 (3S)-citramalyl-CoA => pyruvate + acetyl-CoA (source version: Rhea release 142, 2026-09-02; digest covers complete compressed original RDF)
MetaCyc:CITRAMALYL-COA-LYASE-RXN CITRAMALYL-COA-LYASE-RXN (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
UniProtKB:A9W9X0 biotin carboxylase (source version: Complete original UniProt JSON retrieved 2026-10-11; entry version 97, sequence version 1, annotation date 2026-09-02)
UniProtKB:A9WBQ8 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (source version: Complete original UniProt JSON retrieved 2026-10-11; entry version 113, sequence version 1, annotation date 2026-09-02)
UniProtKB:A9WBQ9 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (source version: Complete original UniProt JSON retrieved 2026-10-11; entry version 87, sequence version 1, annotation date 2026-09-02)
UniProtKB:Q6QQP7 Malonyl-CoA reductase (source version: Complete original UniProt JSON retrieved 2026-10-11; entry version 90, sequence version 1, annotation date 2025-06-18)
UniProtKB:Q8VRG6 acetate--CoA ligase (source version: Complete original UniProt JSON retrieved 2026-10-11; entry version 105, sequence version 2, annotation date 2026-09-02)
UniProtKB:S5N020 Malyl-CoA/beta-methylmalyl-CoA/citramalyl-CoA lyase (source version: Complete original UniProt JSON retrieved 2026-10-11; entry version 45, sequence version 1, annotation date 2026-06-10)
UniProtKB:A9WC34 Beta-methylmalyl-CoA dehydratase (source version: Complete original UniProt JSON retrieved 2026-10-11; entry version 75, sequence version 1, annotation date 2026-06-10)
UniProtKB:A9WC36 2-methylfumaryl-CoA isomerase (source version: Complete original UniProt JSON retrieved 2026-10-11; entry version 79, sequence version 1, annotation date 2026-06-10)
UniProtKB:A9WC41 Mesaconyl-C(4)-CoA hydratase (source version: Complete original UniProt JSON retrieved 2026-10-11; entry version 67, sequence version 1, annotation date 2026-09-02)
MetaCyc:CPLX-7550 malonyl CoA reductase (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:CPLX-7549 L-malyl CoA lyase (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:CPLX-8468 β-methylmalyl-CoA dehydratase (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:CPLX-8466 mesaconyl-C1-CoA-C4-CoA transferase (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:CPLX-8467 mesaconyl-C4-CoA hydratase (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:MONOMER-13606 malonyl CoA reductase subunit (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:MONOMER-13604 L-malyl CoA lyase subunit (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:CPLX-7552 propionyl-CoA synthase (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:MONOMER-13612 propionyl-CoA synthase subunit (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:MONOMER-17292 β-methylmalyl-CoA dehydratase monomer (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:MONOMER-17293 mesaconyl-C1-CoA-C4-CoA transferase monomer (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:MONOMER-17294 mesaconyl-C4-CoA hydratase monomer (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:CPD-9409 (2R,3S)-β-methylmalyl-CoA (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)
MetaCyc:CPD-627 (3S)-citramalyl-CoA (source version: MetaCyc 22.5; complete original XML retrieved 2026-10-11)

Curation history

2026-10-11T10:03:16Z codex
- CREATE — Add glyoxylate assimilation
  Curated the complete 13-activity Chloroflexus aurantiacus glyoxylate-assimilation cycle, retaining the shared acetyl-CoA-to-propionyl-CoA branch, exact strain/protein evidence, and the primary-supported stereochemical correction to a conflicting native structure. Maintained record MetaCyc:PWY-5744; creation bytes SHA256 b28136c61fae7fcf2b9673d176e74d0c001b6cfedeab652ba75ab4ac01aa01e5. Original-source projections, replay and identifier decisions are in research/pathway_additions/2026-10-11-next-206/. Duplicate searches included ignored and hidden files. Independent review and full integration gates are separate subsequent checks, not claimed by this creation event.
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2026-10-11T10:25:01Z codex
- EDIT — Correct native glyoxylate hydration evidence attribution
  Corrected only glyox-edge-066 native RXN8965 source assertion and locator, replacing unrelated biotin-carboxyl-transfer wording with the original mesaconyl-C4-CoA hydration attribution. The selected Rhea38273 chemistry, all endpoints, identities and other evidence remain unchanged. Exact prior/final bytes and source checks are retained in research/pathway_additions/2026-10-11-next-206/glyoxylate-provenance-fix.json and review-correction-audit.json. Final record SHA256 218feaba4c438ac1f7dff5765e5993153040fa1b0cc80b135440ad4ece8622ea. Initial review bundles and inspected input archives are preserved. This event records the supported edit and author checks; independent successor disposition and final coordinated repository gates are subsequent checks.
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