superpathway of menaquinol-8 biosynthesis I

MetaCyc:PWY-5838

The complete E. coli K-12 classical menaquinol-8 superpathway contains ten reactions: seven steps convert chorismate to 1,4-dihydroxy-2-naphthoate, IspB extends farnesyl diphosphate with five isopentenyl diphosphates, and MenA and UbiE prenylate and methylate the naphthalenediol to menaquinol-8. This is the full native PWY-5838 boundary, including its octaprenyl donor branch; upstream chorismate, IPP and farnesyl diphosphate synthesis and downstream respiratory redox cycling are separate. MenF is the dedicated isochorismate synthase. MenD forms SEPHCHC, MenH releases pyruvate, and MenI hydrolyzes DHNA-CoA; older assignments that collapsed these activities are superseded. K-12 protein annotation and MG1655 native frames are distinguished from primary experiments in specific K-12 derivatives, including XL1 Blue and BW25113. The quinol endpoint follows exact native reactions and current Rhea chemistry; older quinone extraction studies do not directly establish intracellular redox occupancy. Native MenE tetramer, reviewed homodimer by similarity and monomeric inhibitor-bound crystal assemblies disagree, so its edge expresses supported function without resolving assembly. MenC, MenA and UbiE functional edges likewise do not assert isolated-monomer sufficiency. UbiE membership in the ubiquinone metabolon is not transferred to this pathway. Magnesium and manganese alternatives for MenD and inferred IspB magnesium binding retain their evidence limits; bicarbonate is a supported MenB cofactor, not a stoichiometric substrate.

Pathway type
menaquinol 8 biosynthesis
Organism scope

Read the source YAML

Pathway network

55 nodes · 85 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Pathway network: superpathway of menaquinol-8 biosynthesis I 55 nodes · 85 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. CHEBI:29748 — consumes → RHEA:18986; Evidence: RHEA:18986consumes RHEA:18986 — produces → CHEBI:29780; Evidence: RHEA:18986produces CHEBI:29780 — consumes → RHEA:25594; Evidence: RHEA:25594consumes CHEBI:16810 — consumes → RHEA:25594; Evidence: RHEA:25594consumes CHEBI:15378 — consumes → RHEA:25594; Evidence: RHEA:25594consumes RHEA:25594 — produces → CHEBI:58818; Evidence: RHEA:25594produces RHEA:25594 — produces → CHEBI:16526; Evidence: RHEA:25594produces CHEBI:58818 — consumes → RHEA:25598; Evidence: RHEA:25598consumes RHEA:25598 — produces → CHEBI:58689; Evidence: RHEA:25598produces RHEA:25598 — produces → CHEBI:15361; Evidence: RHEA:25598produces CHEBI:58689 — consumes → RHEA:10197; Evidence: RHEA:10197consumes RHEA:10197 — produces → CHEBI:18325; Evidence: RHEA:10197produces RHEA:10197 — produces → CHEBI:15377; Evidence: RHEA:10197produces CHEBI:18325 — consumes → RHEA:17010; Evidence: RHEA:17010consumes CHEBI:30616 — consumes → RHEA:17010; Evidence: RHEA:17010consumes CHEBI:57287 — consumes → RHEA:17010; Evidence: RHEA:17010consumes RHEA:17010 — produces → CHEBI:57364; Evidence: RHEA:17010produces RHEA:17010 — produces → CHEBI:456215; Evidence: RHEA:17010produces RHEA:17010 — produces → CHEBI:33019; Evidence: RHEA:17010produces CHEBI:57364 — consumes → RHEA:26563; Evidence: RHEA:26563consumes CHEBI:15378 — consumes → RHEA:26563; Evidence: RHEA:26563consumes RHEA:26563 — produces → CHEBI:58897; Evidence: RHEA:26563produces RHEA:26563 — produces → CHEBI:15377; Evidence: RHEA:26563produces CHEBI:58897 — consumes → RHEA:26310; Evidence: RHEA:26310consumes CHEBI:15377 — consumes → RHEA:26310; Evidence: RHEA:26310consumes RHEA:26310 — produces → CHEBI:11173; Evidence: RHEA:26310produces RHEA:26310 — produces → CHEBI:57287; Evidence: RHEA:26310produces RHEA:26310 — produces → CHEBI:15378; Evidence: RHEA:26310produces CHEBI:128769 — consumes → RHEA:27799; Evidence: RHEA:27799consumes CHEBI:175763 — consumes → RHEA:27799; Evidence: RHEA:27799consumes RHEA:27799 — produces → CHEBI:57711; Evidence: RHEA:27799produces RHEA:27799 — produces → CHEBI:33019; Evidence: RHEA:27799produces CHEBI:57711 — consumes → RHEA:30100; Evidence: RHEA:30100consumes CHEBI:11173 — consumes → RHEA:30100; Evidence: RHEA:30100consumes CHEBI:15378 — consumes → RHEA:30100; Evidence: RHEA:30100consumes RHEA:30100 — produces → CHEBI:61873; Evidence: RHEA:30100produces RHEA:30100 — produces → CHEBI:16526; Evidence: RHEA:30100produces RHEA:30100 — produces → CHEBI:33019; Evidence: RHEA:30100produces CHEBI:61873 — consumes → RHEA:30064; Evidence: RHEA:30064consumes CHEBI:59789 — consumes → RHEA:30064; Evidence: RHEA:30064consumes RHEA:30064 — produces → CHEBI:61684; Evidence: RHEA:30064produces RHEA:30064 — produces → CHEBI:57856; Evidence: RHEA:30064produces RHEA:30064 — produces → CHEBI:15378; Evidence: RHEA:30064produces MetaCyc:MENF-CPLX — catalyzes → RHEA:18986; Evidence: UniProtKB:P38051, RHEA:18986, MetaCyc:MENF-CPLX, PMID:9150206, PMID:9795253catalyzes UniProtKB:P38051 — part_of → MetaCyc:MENF-CPLX; Evidence: MetaCyc:MENF-CPLX, MetaCyc:MENF-MONOMER, UniProtKB:P38051part of MetaCyc:MENF-CPLX — has_cofactor → CHEBI:18420; Evidence: UniProtKB:P38051has cofactor RHEA:18986 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:PWY-5837, MetaCyc:ISOCHORSYN-RXN, RHEA:18986, MetaCyc:PWY-5838, UniProtKB:P38051, PMID:23564174part of MetaCyc:CPLX0-7525 — catalyzes → RHEA:25594; Evidence: UniProtKB:P17109, RHEA:25594, MetaCyc:CPLX0-7525, PMID:17760421catalyzes UniProtKB:P17109 — part_of → MetaCyc:CPLX0-7525; Evidence: MetaCyc:CPLX0-7525, MetaCyc:MEND-MONOMER, UniProtKB:P17109part of MetaCyc:CPLX0-7525 — has_cofactor → CHEBI:18420; Metal alternatives do not imply simultaneous magnesium and manganese occupancy.; Evidence: UniProtKB:P17109has cofactor MetaCyc:CPLX0-7525 — has_cofactor → CHEBI:29035; Metal alternatives do not imply simultaneous magnesium and manganese occupancy.; Evidence: UniProtKB:P17109, PMID:14621995has cofactor MetaCyc:CPLX0-7525 — has_cofactor → CHEBI:58937; Evidence: UniProtKB:P17109, PMID:14621995has cofactor RHEA:25594 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:PWY-5837, MetaCyc:2.5.1.64-RXN, RHEA:25594part of UniProtKB:P37355 — catalyzes → RHEA:25598; Monomeric MenH catalyzes the SHCHC-forming elimination; it is not assigned the later DHNA-CoA hydrolysis.; Evidence: UniProtKB:P37355, RHEA:25598, MetaCyc:EG12438-MONOMER, PMID:18284213, PMID:23637813catalyzes RHEA:25598 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:PWY-5837, MetaCyc:RXN-9310, RHEA:25598part of UniProtKB:P29208 — enables → RHEA:10197; Functional activity is represented without asserting isolated-monomer sufficiency.; Evidence: UniProtKB:P29208, RHEA:10197, MetaCyc:O-SUCCINYLBENZOATE-COA-SYN-MONOMER, PMID:10978150enables UniProtKB:P29208 — has_cofactor → CHEBI:18420; Evidence: UniProtKB:P29208has cofactor RHEA:10197 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:PWY-5837, MetaCyc:O-SUCCINYLBENZOATE-COA-SYN-RXN, RHEA:10197part of UniProtKB:P37353 — enables → RHEA:17010; Supported MenE function; native tetramer and reviewed homodimer-by-similarity assignments conflict, while inhibitor-bound author/PISA structures are monomeric. No unique oligomer is asserted.; Evidence: UniProtKB:P37353, RHEA:17010, MetaCyc:MENE-CPLX, PMID:26394156, MetaCyc:MENE-CPLX, UniProtKB:P37353, DOI:10.2210/pdb6NJ0/pdb, DOI:10.2210/pdb5C5H/pdb, PMID:30912442, PMID:26394156enables RHEA:17010 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:PWY-5837, MetaCyc:O-SUCCINYLBENZOATE-COA-LIG-RXN, RHEA:17010part of MetaCyc:CPLX0-7882 — catalyzes → RHEA:26563; Evidence: UniProtKB:P0ABU0, RHEA:26563, MetaCyc:CPLX0-7882, PMID:20643650catalyzes UniProtKB:P0ABU0 — part_of → MetaCyc:CPLX0-7882; Evidence: MetaCyc:CPLX0-7882, MetaCyc:NAPHTHOATE-SYN-MONOMER, UniProtKB:P0ABU0part of MetaCyc:CPLX0-7882 — has_cofactor → CHEBI:17544; Evidence: UniProtKB:P0ABU0, PMID:20643650has cofactor RHEA:26563 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:PWY-5837, MetaCyc:NAPHTHOATE-SYN-RXN, RHEA:26563part of MetaCyc:CPLX0-8128 — catalyzes → RHEA:26310; Evidence: UniProtKB:P77781, RHEA:26310, MetaCyc:CPLX0-8128, PMID:23564174catalyzes UniProtKB:P77781 — part_of → MetaCyc:CPLX0-8128; Evidence: MetaCyc:CPLX0-8128, MetaCyc:G6912-MONOMER, UniProtKB:P77781, PMID:25010423part of RHEA:26310 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:PWY-5837, MetaCyc:RXN-9311, RHEA:26310part of MetaCyc:CPLX0-7426 — catalyzes → RHEA:27799; Evidence: UniProtKB:P0AD57, RHEA:27799, MetaCyc:CPLX0-7426, PMID:8037730catalyzes UniProtKB:P0AD57 — part_of → MetaCyc:CPLX0-7426; Evidence: MetaCyc:CPLX0-7426, MetaCyc:OPPSYN-MONOMER, PMID:11108713part of MetaCyc:CPLX0-7426 — has_cofactor → CHEBI:18420; The IspB magnesium assignment and two ions per subunit are by similarity.; Evidence: UniProtKB:P0AD57has cofactor RHEA:27799 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:PWY-5783, MetaCyc:RXN-8992, RHEA:27799part of UniProtKB:P32166 — enables → RHEA:30100; Functional activity is represented without asserting isolated-monomer sufficiency.; Evidence: UniProtKB:P32166, RHEA:30100, RHEA:30100, MetaCyc:DMK-MONOMER, PMID:9573170enables RHEA:30100 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:PWY-5852, MetaCyc:DMK-RXN, RHEA:30100part of UniProtKB:P0A887 — enables → RHEA:30064; Fixed-chain menaquinol methylation follows native and Rhea chemistry. The ubiquinone Ubi metabolon is not asserted as its required assembly here.; Evidence: UniProtKB:P0A887, RHEA:30064, RHEA:30064, MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER, PMID:9045837enables RHEA:30064 — part_of → MetaCyc:PWY-5838; Evidence: MetaCyc:PWY-5838, MetaCyc:MENAQUINONESYN-PWY, MetaCyc:ADOMET-DMK-METHYLTRANSFER-RXN, RHEA:30064part of UniProtKB:P32166 — located_in → GO:0005886; Evidence: UniProtKB:P32166located in RHEA:18986 — provides_input_for → RHEA:25594; Curated continuity through CHEBI:29780; no physical channeling or flux magnitude is asserted.; Evidence: RHEA:18986, RHEA:25594provides input for RHEA:25594 — provides_input_for → RHEA:25598; Curated continuity through CHEBI:58818; no physical channeling or flux magnitude is asserted.; Evidence: RHEA:25594, RHEA:25598provides input for RHEA:25598 — provides_input_for → RHEA:10197; Curated continuity through CHEBI:58689; no physical channeling or flux magnitude is asserted.; Evidence: RHEA:25598, RHEA:10197provides input for RHEA:10197 — provides_input_for → RHEA:17010; Curated continuity through CHEBI:18325; no physical channeling or flux magnitude is asserted.; Evidence: RHEA:10197, RHEA:17010provides input for RHEA:17010 — provides_input_for → RHEA:26563; Curated continuity through CHEBI:57364; no physical channeling or flux magnitude is asserted.; Evidence: RHEA:17010, RHEA:26563provides input for RHEA:26563 — provides_input_for → RHEA:26310; Curated continuity through CHEBI:58897; no physical channeling or flux magnitude is asserted.; Evidence: RHEA:26563, RHEA:26310provides input for RHEA:26310 — provides_input_for → RHEA:30100; Curated continuity through CHEBI:11173; no physical channeling or flux magnitude is asserted.; Evidence: RHEA:26310, RHEA:30100provides input for RHEA:27799 — provides_input_for → RHEA:30100; Curated continuity through CHEBI:57711; no physical channeling or flux magnitude is asserted.; Evidence: RHEA:27799, RHEA:30100provides input for RHEA:30100 — provides_input_for → RHEA:30064; Curated continuity through CHEBI:61873; no physical channeling or flux magnitude is asserted.; Evidence: RHEA:30100, RHEA:30064provides input for chorismate(2-); CHEBI:29748; Declared type: small_molecule small molecule chorismate(2-) isochorismate(2-); CHEBI:29780; Declared type: small_molecule small molecule isochorismate(2-) 2-oxoglutarate(2-); CHEBI:16810; Declared type: small_molecule small molecule 2-oxoglutarate(2-) hydron; CHEBI:15378; Declared type: small_molecule small molecule hydron (1R,2S,5S,6S)-2-(3-carboxylatopropanoyl)-5-[(1-carboxylatovinyl)oxy]-6-hydroxycyclohex-3-ene-1-carboxylate; CHEBI:58818; Declared type: small_molecule small molecule (1R,2S,5S,6S)-2-(3- carboxylatopropanoyl)-5-[(1- carboxylatovinyl)oxy]-6- hydroxycyclohex-3-ene-1- carboxylate carbon dioxide; CHEBI:16526; Declared type: small_molecule small molecule carbon dioxide (1R,6R)-2-(3-carboxylatopropanoyl)-6-hydroxycyclohexa-2,4-diene-1-carboxylate; CHEBI:58689; Declared type: small_molecule small molecule (1R,6R)-2-(3- carboxylatopropanoyl)-6- hydroxycyclohexa-2,4-diene- 1-carboxylate pyruvate; CHEBI:15361; Declared type: small_molecule small molecule pyruvate 2-succinatobenzoate; CHEBI:18325; Declared type: small_molecule small molecule 2-succinatobenzoate water; CHEBI:15377; Declared type: small_molecule small molecule water ATP(4-); CHEBI:30616; Declared type: small_molecule small molecule ATP(4-) coenzyme A(4-); CHEBI:57287; Declared type: small_molecule small molecule coenzyme A(4-) 4-(2-carboxyphenyl)-4-oxobutanoyl-CoA(5-); CHEBI:57364; Declared type: small_molecule small molecule 4-(2-carboxyphenyl)-4- oxobutanoyl-CoA(5-) adenosine 5'-monophosphate(2-); CHEBI:456215; Declared type: small_molecule small molecule adenosine 5'-monophosphate(2-) diphosphate(3-); CHEBI:33019; Declared type: small_molecule small molecule diphosphate(3-) 1,4-dihydroxy-2-naphthoyl-CoA(4-); CHEBI:58897; Declared type: small_molecule small molecule 1,4-dihydroxy-2-naphthoyl- CoA(4-) 1,4-dihydroxy-2-naphthoate; CHEBI:11173; Declared type: small_molecule small molecule 1,4-dihydroxy-2-naphthoate isopentenyl diphosphate(3-); CHEBI:128769; Declared type: small_molecule small molecule isopentenyl diphosphate(3-) 2-trans,6-trans-farnesyl diphosphate(3-); CHEBI:175763; Declared type: small_molecule small molecule 2-trans,6-trans-farnesyl diphosphate(3-) all-trans-octaprenyl diphosphate(3-); CHEBI:57711; Declared type: small_molecule small molecule all-trans-octaprenyl diphosphate(3-) 2-demethylmenaquinol-8; CHEBI:61873; Declared type: lipid lipid 2-demethylmenaquinol-8 S-adenosyl-L-methionine zwitterion; CHEBI:59789; Declared type: small_molecule small molecule S-adenosyl-L-methionine zwitterion menaquinol-8; CHEBI:61684; Declared type: lipid lipid menaquinol-8 S-adenosyl-L-homocysteine zwitterion; CHEBI:57856; Declared type: small_molecule small molecule S-adenosyl-L-homocysteine zwitterion magnesium(2+); CHEBI:18420; Declared type: cofactor cofactor magnesium(2+) manganese(2+); CHEBI:29035; Declared type: cofactor cofactor manganese(2+) thiamine(1+) diphosphate(3-); CHEBI:58937; Declared type: cofactor cofactor thiamine(1+) diphosphate(3-) hydrogencarbonate; CHEBI:17544; Declared type: cofactor cofactor hydrogencarbonate plasma membrane; GO:0005886; Declared type: cellular_component cellular component plasma membrane Isochorismate synthase MenF; UniProtKB:P38051; Declared type: protein protein Isochorismate synthase MenF 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase; UniProtKB:P17109; Declared type: protein protein 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1- carboxylate synthase 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase; UniProtKB:P37355; Declared type: protein protein 2-succinyl-6-hydroxy-2,4- cyclohexadiene-1-carboxylate synthase o-succinylbenzoate synthase; UniProtKB:P29208; Declared type: protein protein o-succinylbenzoate synthase 2-succinylbenzoate--CoA ligase; UniProtKB:P37353; Declared type: protein protein 2-succinylbenzoate--CoA ligase 1,4-dihydroxy-2-naphthoyl-CoA synthase; UniProtKB:P0ABU0; Declared type: protein protein 1,4-dihydroxy-2-naphthoyl- CoA synthase 1,4-dihydroxy-2-naphthoyl-CoA hydrolase; UniProtKB:P77781; Declared type: protein protein 1,4-dihydroxy-2-naphthoyl- CoA hydrolase Octaprenyl diphosphate synthase; UniProtKB:P0AD57; Declared type: protein protein Octaprenyl diphosphate synthase 1,4-dihydroxy-2-naphthoate octaprenyltransferase; UniProtKB:P32166; Declared type: protein protein 1,4-dihydroxy-2-naphthoate octaprenyltransferase Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE; UniProtKB:P0A887; Declared type: protein protein Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE isochorismate synthase MenF; MetaCyc:MENF-CPLX; Declared type: complex complex isochorismate synthase MenF 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase; MetaCyc:CPLX0-7525; Declared type: complex complex 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1- carboxylate synthase 1,4-dihydroxy-2-naphthoyl-CoA synthase; MetaCyc:CPLX0-7882; Declared type: complex complex 1,4-dihydroxy-2-naphthoyl- CoA synthase 1,4-dihydroxy-2-naphthoyl-CoA hydrolase; MetaCyc:CPLX0-8128; Declared type: complex complex 1,4-dihydroxy-2-naphthoyl- CoA hydrolase all-trans-octaprenyl-diphosphate synthase; MetaCyc:CPLX0-7426; Declared type: complex complex all-trans-octaprenyl- diphosphate synthase chorismate => isochorismate; RHEA:18986; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity chorismate => isochorismate isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2; RHEA:25594; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity isochorismate + 2-oxoglutarate + H(+) => 5- enolpyruvoyl-6-hydroxy-2- succinyl- cyclohex-3-ene-1-carboxylate + CO2 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate; RHEA:25598; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity 5-enolpyruvoyl-6-hydroxy-2- succinyl- cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2- succinyl-cyclohexa-2,4- diene-1-carboxylate + pyruvate (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate => 2-succinylbenzoate + H2O; RHEA:10197; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity (1R,6R)-6-hydroxy-2- succinyl-cyclohexa-2,4- diene-1-carboxylate => 2-succinylbenzoate + H2O 2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate; RHEA:17010; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity 2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate 2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O; RHEA:26563; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity 2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl- CoA + H2O 1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+); RHEA:26310; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity 1,4-dihydroxy-2-naphthoyl- CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+) 5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate; RHEA:27799; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity 5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate; RHEA:30100; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate 2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+); RHEA:30064; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity 2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L- homocysteine + H(+) superpathway of menaquinol-8 biosynthesis I; MetaCyc:PWY-5838; Declared type: pathway pathway superpathway of menaquinol-8 biosynthesis I

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Escherichia coli K-12
NCBITaxon:83333		taxon
chorismate(2-)
CHEBI:29748		small molecule
isochorismate(2-)
CHEBI:29780		small molecule
2-oxoglutarate(2-)
CHEBI:16810		small molecule
hydron
CHEBI:15378		small molecule
(1R,2S,5S,6S)-2-(3-carboxylatopropanoyl)-5-[(1-carboxylatovinyl)oxy]-6-hydroxycyclohex-3-ene-1-carboxylate
CHEBI:58818		small molecule
carbon dioxide
CHEBI:16526		small molecule
(1R,6R)-2-(3-carboxylatopropanoyl)-6-hydroxycyclohexa-2,4-diene-1-carboxylate
CHEBI:58689		small molecule
pyruvate
CHEBI:15361		small molecule
2-succinatobenzoate
CHEBI:18325		small molecule
water
CHEBI:15377		small molecule
ATP(4-)
CHEBI:30616		small molecule
coenzyme A(4-)
CHEBI:57287		small molecule
4-(2-carboxyphenyl)-4-oxobutanoyl-CoA(5-)
CHEBI:57364		small molecule
adenosine 5'-monophosphate(2-)
CHEBI:456215		small molecule
diphosphate(3-)
CHEBI:33019		small molecule
1,4-dihydroxy-2-naphthoyl-CoA(4-)
CHEBI:58897		small molecule
1,4-dihydroxy-2-naphthoate
CHEBI:11173		small molecule
isopentenyl diphosphate(3-)
CHEBI:128769		small molecule
2-trans,6-trans-farnesyl diphosphate(3-)
CHEBI:175763		small molecule
all-trans-octaprenyl diphosphate(3-)
CHEBI:57711		small molecule
2-demethylmenaquinol-8
CHEBI:61873		lipid
S-adenosyl-L-methionine zwitterion
CHEBI:59789		small molecule
menaquinol-8
CHEBI:61684		lipid
S-adenosyl-L-homocysteine zwitterion
CHEBI:57856		small molecule
magnesium(2+)
CHEBI:18420		cofactor
manganese(2+)
CHEBI:29035		cofactor
thiamine(1+) diphosphate(3-)
CHEBI:58937		cofactor
hydrogencarbonate
CHEBI:17544		cofactor
plasma membrane
GO:0005886		cellular component
Isochorismate synthase MenF
UniProtKB:P38051		protein
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
UniProtKB:P17109		protein
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
UniProtKB:P37355		protein
o-succinylbenzoate synthase
UniProtKB:P29208		protein
2-succinylbenzoate--CoA ligase
UniProtKB:P37353		protein
1,4-dihydroxy-2-naphthoyl-CoA synthase
UniProtKB:P0ABU0		protein
1,4-dihydroxy-2-naphthoyl-CoA hydrolase
UniProtKB:P77781		protein
Octaprenyl diphosphate synthase
UniProtKB:P0AD57		protein
1,4-dihydroxy-2-naphthoate octaprenyltransferase
UniProtKB:P32166		protein
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE
UniProtKB:P0A887		protein
isochorismate synthase MenF
MetaCyc:MENF-CPLX		complex
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
MetaCyc:CPLX0-7525		complex
1,4-dihydroxy-2-naphthoyl-CoA synthase
MetaCyc:CPLX0-7882		complex
1,4-dihydroxy-2-naphthoyl-CoA hydrolase
MetaCyc:CPLX0-8128		complex
all-trans-octaprenyl-diphosphate synthase
MetaCyc:CPLX0-7426		complex
chorismate => isochorismate
RHEA:18986		molecular activityleft to right
isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594		molecular activityleft to right
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate
RHEA:25598		molecular activityleft to right
(1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate => 2-succinylbenzoate + H2O
RHEA:10197		molecular activityleft to right
2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010		molecular activityleft to right
2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O
RHEA:26563		molecular activityleft to right
1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310		molecular activityleft to right
5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate
RHEA:27799		molecular activityleft to right
all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100		molecular activityleft to right
2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+)
RHEA:30064		molecular activityleft to right

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
chorismate(2-)
CHEBI:29748		consumes

chorismate => isochorismate
RHEA:18986
  • RHEA:18986

    Source assertion: RHEA:18986 assigns CHEBI:29748 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/18986 rh:substrates http://rdf.rhea-db.org/18985_L; http://rdf.rhea-db.org/18985_L rh:contains1 http://rdf.rhea-db.org/Participant_18985_compound_3304; http://rdf.rhea-db.org/Participant_18985_compound_3304 rh:compound http://rdf.rhea-db.org/Compound_3304; http://rdf.rhea-db.org/Compound_3304 rh:accession; rh:chebi
chorismate => isochorismate
RHEA:18986		produces

isochorismate(2-)
CHEBI:29780
  • RHEA:18986

    Source assertion: RHEA:18986 assigns CHEBI:29780 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/18986 rh:products http://rdf.rhea-db.org/18985_R; http://rdf.rhea-db.org/18985_R rh:contains1 http://rdf.rhea-db.org/Participant_18985_compound_3577; http://rdf.rhea-db.org/Participant_18985_compound_3577 rh:compound http://rdf.rhea-db.org/Compound_3577; http://rdf.rhea-db.org/Compound_3577 rh:accession; rh:chebi
isochorismate(2-)
CHEBI:29780		consumes

isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594
  • RHEA:25594

    Source assertion: RHEA:25594 assigns CHEBI:29780 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/25594 rh:substrates http://rdf.rhea-db.org/25593_L; http://rdf.rhea-db.org/25593_L rh:contains1 http://rdf.rhea-db.org/Participant_25593_compound_3577; http://rdf.rhea-db.org/Participant_25593_compound_3577 rh:compound http://rdf.rhea-db.org/Compound_3577; http://rdf.rhea-db.org/Compound_3577 rh:accession; rh:chebi
2-oxoglutarate(2-)
CHEBI:16810		consumes

isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594
  • RHEA:25594

    Source assertion: RHEA:25594 assigns CHEBI:16810 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/25594 rh:substrates http://rdf.rhea-db.org/25593_L; http://rdf.rhea-db.org/25593_L rh:contains1 http://rdf.rhea-db.org/Participant_25593_compound_2975; http://rdf.rhea-db.org/Participant_25593_compound_2975 rh:compound http://rdf.rhea-db.org/Compound_2975; http://rdf.rhea-db.org/Compound_2975 rh:accession; rh:chebi
hydron
CHEBI:15378		consumes

isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594
  • RHEA:25594

    Source assertion: RHEA:25594 assigns CHEBI:15378 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/25594 rh:substrates http://rdf.rhea-db.org/25593_L; http://rdf.rhea-db.org/25593_L rh:contains1 http://rdf.rhea-db.org/Participant_25593_compound_3249; http://rdf.rhea-db.org/Participant_25593_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; http://rdf.rhea-db.org/Compound_3249 rh:accession; rh:chebi
isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594		produces

(1R,2S,5S,6S)-2-(3-carboxylatopropanoyl)-5-[(1-carboxylatovinyl)oxy]-6-hydroxycyclohex-3-ene-1-carboxylate
CHEBI:58818
  • RHEA:25594

    Source assertion: RHEA:25594 assigns CHEBI:58818 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/25594 rh:products http://rdf.rhea-db.org/25593_R; http://rdf.rhea-db.org/25593_R rh:contains1 http://rdf.rhea-db.org/Participant_25593_compound_5683; http://rdf.rhea-db.org/Participant_25593_compound_5683 rh:compound http://rdf.rhea-db.org/Compound_5683; http://rdf.rhea-db.org/Compound_5683 rh:accession; rh:chebi
isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594		produces

carbon dioxide
CHEBI:16526
  • RHEA:25594

    Source assertion: RHEA:25594 assigns CHEBI:16526 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/25594 rh:products http://rdf.rhea-db.org/25593_R; http://rdf.rhea-db.org/25593_R rh:contains1 http://rdf.rhea-db.org/Participant_25593_compound_4391; http://rdf.rhea-db.org/Participant_25593_compound_4391 rh:compound http://rdf.rhea-db.org/Compound_4391; http://rdf.rhea-db.org/Compound_4391 rh:accession; rh:chebi
(1R,2S,5S,6S)-2-(3-carboxylatopropanoyl)-5-[(1-carboxylatovinyl)oxy]-6-hydroxycyclohex-3-ene-1-carboxylate
CHEBI:58818		consumes

5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate
RHEA:25598
  • RHEA:25598

    Source assertion: RHEA:25598 assigns CHEBI:58818 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/25598 rh:substrates http://rdf.rhea-db.org/25597_L; http://rdf.rhea-db.org/25597_L rh:contains1 http://rdf.rhea-db.org/Participant_25597_compound_5683; http://rdf.rhea-db.org/Participant_25597_compound_5683 rh:compound http://rdf.rhea-db.org/Compound_5683; http://rdf.rhea-db.org/Compound_5683 rh:accession; rh:chebi
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate
RHEA:25598		produces

(1R,6R)-2-(3-carboxylatopropanoyl)-6-hydroxycyclohexa-2,4-diene-1-carboxylate
CHEBI:58689
  • RHEA:25598

    Source assertion: RHEA:25598 assigns CHEBI:58689 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/25598 rh:products http://rdf.rhea-db.org/25597_R; http://rdf.rhea-db.org/25597_R rh:contains1 http://rdf.rhea-db.org/Participant_25597_compound_5418; http://rdf.rhea-db.org/Participant_25597_compound_5418 rh:compound http://rdf.rhea-db.org/Compound_5418; http://rdf.rhea-db.org/Compound_5418 rh:accession; rh:chebi
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate
RHEA:25598		produces

pyruvate
CHEBI:15361
  • RHEA:25598

    Source assertion: RHEA:25598 assigns CHEBI:15361 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/25598 rh:products http://rdf.rhea-db.org/25597_R; http://rdf.rhea-db.org/25597_R rh:contains1 http://rdf.rhea-db.org/Participant_25597_compound_3719; http://rdf.rhea-db.org/Participant_25597_compound_3719 rh:compound http://rdf.rhea-db.org/Compound_3719; http://rdf.rhea-db.org/Compound_3719 rh:accession; rh:chebi
(1R,6R)-2-(3-carboxylatopropanoyl)-6-hydroxycyclohexa-2,4-diene-1-carboxylate
CHEBI:58689		consumes

(1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate => 2-succinylbenzoate + H2O
RHEA:10197
  • RHEA:10197

    Source assertion: RHEA:10197 assigns CHEBI:58689 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/10197 rh:substrates http://rdf.rhea-db.org/10196_L; http://rdf.rhea-db.org/10196_L rh:contains1 http://rdf.rhea-db.org/Participant_10196_compound_5418; http://rdf.rhea-db.org/Participant_10196_compound_5418 rh:compound http://rdf.rhea-db.org/Compound_5418; http://rdf.rhea-db.org/Compound_5418 rh:accession; rh:chebi
(1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate => 2-succinylbenzoate + H2O
RHEA:10197		produces

2-succinatobenzoate
CHEBI:18325
  • RHEA:10197

    Source assertion: RHEA:10197 assigns CHEBI:18325 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/10197 rh:products http://rdf.rhea-db.org/10196_R; http://rdf.rhea-db.org/10196_R rh:contains1 http://rdf.rhea-db.org/Participant_10196_compound_5241; http://rdf.rhea-db.org/Participant_10196_compound_5241 rh:compound http://rdf.rhea-db.org/Compound_5241; http://rdf.rhea-db.org/Compound_5241 rh:accession; rh:chebi
(1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate => 2-succinylbenzoate + H2O
RHEA:10197		produces

water
CHEBI:15377
  • RHEA:10197

    Source assertion: RHEA:10197 assigns CHEBI:15377 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/10197 rh:products http://rdf.rhea-db.org/10196_R; http://rdf.rhea-db.org/10196_R rh:contains1 http://rdf.rhea-db.org/Participant_10196_compound_1283; http://rdf.rhea-db.org/Participant_10196_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; http://rdf.rhea-db.org/Compound_1283 rh:accession; rh:chebi
2-succinatobenzoate
CHEBI:18325		consumes

2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010
  • RHEA:17010

    Source assertion: RHEA:17010 assigns CHEBI:18325 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/17010 rh:substrates http://rdf.rhea-db.org/17009_L; http://rdf.rhea-db.org/17009_L rh:contains1 http://rdf.rhea-db.org/Participant_17009_compound_5241; http://rdf.rhea-db.org/Participant_17009_compound_5241 rh:compound http://rdf.rhea-db.org/Compound_5241; http://rdf.rhea-db.org/Compound_5241 rh:accession; rh:chebi
ATP(4-)
CHEBI:30616		consumes

2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010
  • RHEA:17010

    Source assertion: RHEA:17010 assigns CHEBI:30616 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/17010 rh:substrates http://rdf.rhea-db.org/17009_L; http://rdf.rhea-db.org/17009_L rh:contains1 http://rdf.rhea-db.org/Participant_17009_compound_6372; http://rdf.rhea-db.org/Participant_17009_compound_6372 rh:compound http://rdf.rhea-db.org/Compound_6372; http://rdf.rhea-db.org/Compound_6372 rh:accession; rh:chebi
coenzyme A(4-)
CHEBI:57287		consumes

2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010
  • RHEA:17010

    Source assertion: RHEA:17010 assigns CHEBI:57287 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/17010 rh:substrates http://rdf.rhea-db.org/17009_L; http://rdf.rhea-db.org/17009_L rh:contains1 http://rdf.rhea-db.org/Participant_17009_compound_1264; http://rdf.rhea-db.org/Participant_17009_compound_1264 rh:compound http://rdf.rhea-db.org/Compound_1264; http://rdf.rhea-db.org/Compound_1264 rh:accession; rh:chebi
2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010		produces

4-(2-carboxyphenyl)-4-oxobutanoyl-CoA(5-)
CHEBI:57364
  • RHEA:17010

    Source assertion: RHEA:17010 assigns CHEBI:57364 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/17010 rh:products http://rdf.rhea-db.org/17009_R; http://rdf.rhea-db.org/17009_R rh:contains1 http://rdf.rhea-db.org/Participant_17009_compound_2208; http://rdf.rhea-db.org/Participant_17009_compound_2208 rh:compound http://rdf.rhea-db.org/Compound_2208; http://rdf.rhea-db.org/Compound_2208 rh:accession; rh:chebi
2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010		produces

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • RHEA:17010

    Source assertion: RHEA:17010 assigns CHEBI:456215 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/17010 rh:products http://rdf.rhea-db.org/17009_R; http://rdf.rhea-db.org/17009_R rh:contains1 http://rdf.rhea-db.org/Participant_17009_compound_2590; http://rdf.rhea-db.org/Participant_17009_compound_2590 rh:compound http://rdf.rhea-db.org/Compound_2590; http://rdf.rhea-db.org/Compound_2590 rh:accession; rh:chebi
2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:17010

    Source assertion: RHEA:17010 assigns CHEBI:33019 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/17010 rh:products http://rdf.rhea-db.org/17009_R; http://rdf.rhea-db.org/17009_R rh:contains1 http://rdf.rhea-db.org/Participant_17009_compound_2106; http://rdf.rhea-db.org/Participant_17009_compound_2106 rh:compound http://rdf.rhea-db.org/Compound_2106; http://rdf.rhea-db.org/Compound_2106 rh:accession; rh:chebi
4-(2-carboxyphenyl)-4-oxobutanoyl-CoA(5-)
CHEBI:57364		consumes

2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O
RHEA:26563
  • RHEA:26563

    Source assertion: RHEA:26563 assigns CHEBI:57364 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/26563 rh:substrates http://rdf.rhea-db.org/26562_L; http://rdf.rhea-db.org/26562_L rh:contains1 http://rdf.rhea-db.org/Participant_26562_compound_2208; http://rdf.rhea-db.org/Participant_26562_compound_2208 rh:compound http://rdf.rhea-db.org/Compound_2208; http://rdf.rhea-db.org/Compound_2208 rh:accession; rh:chebi
hydron
CHEBI:15378		consumes

2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O
RHEA:26563
  • RHEA:26563

    Source assertion: RHEA:26563 assigns CHEBI:15378 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/26563 rh:substrates http://rdf.rhea-db.org/26562_L; http://rdf.rhea-db.org/26562_L rh:contains1 http://rdf.rhea-db.org/Participant_26562_compound_3249; http://rdf.rhea-db.org/Participant_26562_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; http://rdf.rhea-db.org/Compound_3249 rh:accession; rh:chebi
2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O
RHEA:26563		produces

1,4-dihydroxy-2-naphthoyl-CoA(4-)
CHEBI:58897
  • RHEA:26563

    Source assertion: RHEA:26563 assigns CHEBI:58897 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/26563 rh:products http://rdf.rhea-db.org/26562_R; http://rdf.rhea-db.org/26562_R rh:contains1 http://rdf.rhea-db.org/Participant_26562_compound_5962; http://rdf.rhea-db.org/Participant_26562_compound_5962 rh:compound http://rdf.rhea-db.org/Compound_5962; http://rdf.rhea-db.org/Compound_5962 rh:accession; rh:chebi
2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O
RHEA:26563		produces

water
CHEBI:15377
  • RHEA:26563

    Source assertion: RHEA:26563 assigns CHEBI:15377 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/26563 rh:products http://rdf.rhea-db.org/26562_R; http://rdf.rhea-db.org/26562_R rh:contains1 http://rdf.rhea-db.org/Participant_26562_compound_1283; http://rdf.rhea-db.org/Participant_26562_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; http://rdf.rhea-db.org/Compound_1283 rh:accession; rh:chebi
1,4-dihydroxy-2-naphthoyl-CoA(4-)
CHEBI:58897		consumes

1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310
  • RHEA:26310

    Source assertion: RHEA:26310 assigns CHEBI:58897 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/26310 rh:substrates http://rdf.rhea-db.org/26309_L; http://rdf.rhea-db.org/26309_L rh:contains1 http://rdf.rhea-db.org/Participant_26309_compound_5962; http://rdf.rhea-db.org/Participant_26309_compound_5962 rh:compound http://rdf.rhea-db.org/Compound_5962; http://rdf.rhea-db.org/Compound_5962 rh:accession; rh:chebi
water
CHEBI:15377		consumes

1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310
  • RHEA:26310

    Source assertion: RHEA:26310 assigns CHEBI:15377 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/26310 rh:substrates http://rdf.rhea-db.org/26309_L; http://rdf.rhea-db.org/26309_L rh:contains1 http://rdf.rhea-db.org/Participant_26309_compound_1283; http://rdf.rhea-db.org/Participant_26309_compound_1283 rh:compound http://rdf.rhea-db.org/Compound_1283; http://rdf.rhea-db.org/Compound_1283 rh:accession; rh:chebi
1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310		produces

1,4-dihydroxy-2-naphthoate
CHEBI:11173
  • RHEA:26310

    Source assertion: RHEA:26310 assigns CHEBI:11173 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/26310 rh:products http://rdf.rhea-db.org/26309_R; http://rdf.rhea-db.org/26309_R rh:contains1 http://rdf.rhea-db.org/Participant_26309_compound_6107; http://rdf.rhea-db.org/Participant_26309_compound_6107 rh:compound http://rdf.rhea-db.org/Compound_6107; http://rdf.rhea-db.org/Compound_6107 rh:accession; rh:chebi
1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310		produces

coenzyme A(4-)
CHEBI:57287
  • RHEA:26310

    Source assertion: RHEA:26310 assigns CHEBI:57287 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/26310 rh:products http://rdf.rhea-db.org/26309_R; http://rdf.rhea-db.org/26309_R rh:contains1 http://rdf.rhea-db.org/Participant_26309_compound_1264; http://rdf.rhea-db.org/Participant_26309_compound_1264 rh:compound http://rdf.rhea-db.org/Compound_1264; http://rdf.rhea-db.org/Compound_1264 rh:accession; rh:chebi
1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310		produces

hydron
CHEBI:15378
  • RHEA:26310

    Source assertion: RHEA:26310 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/26310 rh:products http://rdf.rhea-db.org/26309_R; http://rdf.rhea-db.org/26309_R rh:contains1 http://rdf.rhea-db.org/Participant_26309_compound_3249; http://rdf.rhea-db.org/Participant_26309_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; http://rdf.rhea-db.org/Compound_3249 rh:accession; rh:chebi
isopentenyl diphosphate(3-)
CHEBI:128769		consumes

5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate
RHEA:27799
  • RHEA:27799

    Source assertion: RHEA:27799 assigns CHEBI:128769 to its substrates with coefficient 5.

    Source locationhttp://rdf.rhea-db.org/27799 rh:substrates http://rdf.rhea-db.org/27798_L; http://rdf.rhea-db.org/27798_L rh:contains5 http://rdf.rhea-db.org/Participant_27798_compound_4425; http://rdf.rhea-db.org/Participant_27798_compound_4425 rh:compound http://rdf.rhea-db.org/Compound_4425; http://rdf.rhea-db.org/Compound_4425 rh:accession; rh:chebi
2-trans,6-trans-farnesyl diphosphate(3-)
CHEBI:175763		consumes

5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate
RHEA:27799
  • RHEA:27799

    Source assertion: RHEA:27799 assigns CHEBI:175763 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/27799 rh:substrates http://rdf.rhea-db.org/27798_L; http://rdf.rhea-db.org/27798_L rh:contains1 http://rdf.rhea-db.org/Participant_27798_compound_1582; http://rdf.rhea-db.org/Participant_27798_compound_1582 rh:compound http://rdf.rhea-db.org/Compound_1582; http://rdf.rhea-db.org/Compound_1582 rh:accession; rh:chebi
5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate
RHEA:27799		produces

all-trans-octaprenyl diphosphate(3-)
CHEBI:57711
  • RHEA:27799

    Source assertion: RHEA:27799 assigns CHEBI:57711 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/27799 rh:products http://rdf.rhea-db.org/27798_R; http://rdf.rhea-db.org/27798_R rh:contains1 http://rdf.rhea-db.org/Participant_27798_compound_1744; http://rdf.rhea-db.org/Participant_27798_compound_1744 rh:compound http://rdf.rhea-db.org/Compound_1744; http://rdf.rhea-db.org/Compound_1744 rh:accession; rh:chebi
5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate
RHEA:27799		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:27799

    Source assertion: RHEA:27799 assigns CHEBI:33019 to its products with coefficient 5.

    Source locationhttp://rdf.rhea-db.org/27799 rh:products http://rdf.rhea-db.org/27798_R; http://rdf.rhea-db.org/27798_R rh:contains5 http://rdf.rhea-db.org/Participant_27798_compound_2106; http://rdf.rhea-db.org/Participant_27798_compound_2106 rh:compound http://rdf.rhea-db.org/Compound_2106; http://rdf.rhea-db.org/Compound_2106 rh:accession; rh:chebi
all-trans-octaprenyl diphosphate(3-)
CHEBI:57711		consumes

all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100
  • RHEA:30100

    Source assertion: RHEA:30100 assigns CHEBI:57711 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30100 rh:substrates http://rdf.rhea-db.org/30099_L; http://rdf.rhea-db.org/30099_L rh:contains1 http://rdf.rhea-db.org/Participant_30099_compound_1744; http://rdf.rhea-db.org/Participant_30099_compound_1744 rh:compound http://rdf.rhea-db.org/Compound_1744; http://rdf.rhea-db.org/Compound_1744 rh:accession; rh:chebi
1,4-dihydroxy-2-naphthoate
CHEBI:11173		consumes

all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100
  • RHEA:30100

    Source assertion: RHEA:30100 assigns CHEBI:11173 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30100 rh:substrates http://rdf.rhea-db.org/30099_L; http://rdf.rhea-db.org/30099_L rh:contains1 http://rdf.rhea-db.org/Participant_30099_compound_6107; http://rdf.rhea-db.org/Participant_30099_compound_6107 rh:compound http://rdf.rhea-db.org/Compound_6107; http://rdf.rhea-db.org/Compound_6107 rh:accession; rh:chebi
hydron
CHEBI:15378		consumes

all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100
  • RHEA:30100

    Source assertion: RHEA:30100 assigns CHEBI:15378 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30100 rh:substrates http://rdf.rhea-db.org/30099_L; http://rdf.rhea-db.org/30099_L rh:contains1 http://rdf.rhea-db.org/Participant_30099_compound_3249; http://rdf.rhea-db.org/Participant_30099_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; http://rdf.rhea-db.org/Compound_3249 rh:accession; rh:chebi
all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100		produces

2-demethylmenaquinol-8
CHEBI:61873
  • RHEA:30100

    Source assertion: RHEA:30100 assigns CHEBI:61873 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30100 rh:products http://rdf.rhea-db.org/30099_R; http://rdf.rhea-db.org/30099_R rh:contains1 http://rdf.rhea-db.org/Participant_30099_compound_6609; http://rdf.rhea-db.org/Participant_30099_compound_6609 rh:compound http://rdf.rhea-db.org/Compound_6609; http://rdf.rhea-db.org/Compound_6609 rh:accession; rh:chebi
all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100		produces

carbon dioxide
CHEBI:16526
  • RHEA:30100

    Source assertion: RHEA:30100 assigns CHEBI:16526 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30100 rh:products http://rdf.rhea-db.org/30099_R; http://rdf.rhea-db.org/30099_R rh:contains1 http://rdf.rhea-db.org/Participant_30099_compound_4391; http://rdf.rhea-db.org/Participant_30099_compound_4391 rh:compound http://rdf.rhea-db.org/Compound_4391; http://rdf.rhea-db.org/Compound_4391 rh:accession; rh:chebi
all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:30100

    Source assertion: RHEA:30100 assigns CHEBI:33019 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30100 rh:products http://rdf.rhea-db.org/30099_R; http://rdf.rhea-db.org/30099_R rh:contains1 http://rdf.rhea-db.org/Participant_30099_compound_2106; http://rdf.rhea-db.org/Participant_30099_compound_2106 rh:compound http://rdf.rhea-db.org/Compound_2106; http://rdf.rhea-db.org/Compound_2106 rh:accession; rh:chebi
2-demethylmenaquinol-8
CHEBI:61873		consumes

2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+)
RHEA:30064
  • RHEA:30064

    Source assertion: RHEA:30064 assigns CHEBI:61873 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30064 rh:substrates http://rdf.rhea-db.org/30063_L; http://rdf.rhea-db.org/30063_L rh:contains1 http://rdf.rhea-db.org/Participant_30063_compound_6609; http://rdf.rhea-db.org/Participant_30063_compound_6609 rh:compound http://rdf.rhea-db.org/Compound_6609; http://rdf.rhea-db.org/Compound_6609 rh:accession; rh:chebi
S-adenosyl-L-methionine zwitterion
CHEBI:59789		consumes

2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+)
RHEA:30064
  • RHEA:30064

    Source assertion: RHEA:30064 assigns CHEBI:59789 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30064 rh:substrates http://rdf.rhea-db.org/30063_L; http://rdf.rhea-db.org/30063_L rh:contains1 http://rdf.rhea-db.org/Participant_30063_compound_6127; http://rdf.rhea-db.org/Participant_30063_compound_6127 rh:compound http://rdf.rhea-db.org/Compound_6127; http://rdf.rhea-db.org/Compound_6127 rh:accession; rh:chebi
2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+)
RHEA:30064		produces

menaquinol-8
CHEBI:61684
  • RHEA:30064

    Source assertion: RHEA:30064 assigns CHEBI:61684 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30064 rh:products http://rdf.rhea-db.org/30063_R; http://rdf.rhea-db.org/30063_R rh:contains1 http://rdf.rhea-db.org/Participant_30063_compound_6557; http://rdf.rhea-db.org/Participant_30063_compound_6557 rh:compound http://rdf.rhea-db.org/Compound_6557; http://rdf.rhea-db.org/Compound_6557 rh:accession; rh:chebi
2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+)
RHEA:30064		produces

S-adenosyl-L-homocysteine zwitterion
CHEBI:57856
  • RHEA:30064

    Source assertion: RHEA:30064 assigns CHEBI:57856 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30064 rh:products http://rdf.rhea-db.org/30063_R; http://rdf.rhea-db.org/30063_R rh:contains1 http://rdf.rhea-db.org/Participant_30063_compound_4281; http://rdf.rhea-db.org/Participant_30063_compound_4281 rh:compound http://rdf.rhea-db.org/Compound_4281; http://rdf.rhea-db.org/Compound_4281 rh:accession; rh:chebi
2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+)
RHEA:30064		produces

hydron
CHEBI:15378
  • RHEA:30064

    Source assertion: RHEA:30064 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/30064 rh:products http://rdf.rhea-db.org/30063_R; http://rdf.rhea-db.org/30063_R rh:contains1 http://rdf.rhea-db.org/Participant_30063_compound_3249; http://rdf.rhea-db.org/Participant_30063_compound_3249 rh:compound http://rdf.rhea-db.org/Compound_3249; http://rdf.rhea-db.org/Compound_3249 rh:accession; rh:chebi
isochorismate synthase MenF
MetaCyc:MENF-CPLX		catalyzes

chorismate => isochorismate
RHEA:18986
  • UniProtKB:P38051

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000255 HAMAP-Rule:MF_01935, ECO:0000269 PubMed:17240978, ECO:0000269 PubMed:8764478, ECO:0000269 PubMed:9150206, ECO:0000269 PubMed:9795253. Its Rhea reaction is RHEA:18985.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:18986

    Source assertion: RHEA:18985 explicitly links the left-to-right directional reaction RHEA:18986.

    Source locationhttp://rdf.rhea-db.org/18985 rh:directionalReaction http://rdf.rhea-db.org/18986; http://rdf.rhea-db.org/18986 rh:substrates; rh:products; rh:equation
  • MetaCyc:MENF-CPLX

    Source assertion: Native MENF-CPLX explicitly links its catalyzed enzymatic reaction to ISOCHORSYN-RXN; this supports the reaction assignment.

    Source locationProtein[@frameid="MENF-CPLX"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:9150206

    Source assertion: Purified recombinant E. coli MenF converts chorismate to isochorismate with magnesium; gel filtration and SDS-PAGE support a homodimer. The abstract described the reaction as irreversible, whereas the later MenF/EntC study found reversibility.

    Source location/resultList/result[id="9150206"]/abstractText
  • PMID:9795253

    Source assertion: The primary abstract distinguishes MenF and EntC using E. coli mutants: menF disruption leaves only trace MK-8 despite intact entC. Purified tagged MenF catalyzes both forward and reverse isomerization.

    Source location/resultList/result[id="9795253"]/abstractText
Isochorismate synthase MenF
UniProtKB:P38051		part_of

isochorismate synthase MenF
MetaCyc:MENF-CPLX
  • MetaCyc:MENF-CPLX

    Source assertion: The native MG1655 complex has 2 copies of MENF-MONOMER.

    Source locationProtein[@frameid="MENF-CPLX"]/component/Protein/@frameid; component/coefficient; species/Organism/@frameid
  • MetaCyc:MENF-MONOMER

    Source assertion: The native protein explicitly unifies with UniProt P38051.

    Source locationProtein[@frameid="MENF-MONOMER"]/dblink[dblink-db="UNIPROT"]/dblink-oid; dblink-relationship
  • UniProtKB:P38051

    Source assertion: SUBUNIT states Homodimer; its evidence codes remain explicit in the original reviewed entry.

    Source location/comments/6/texts
isochorismate synthase MenF
MetaCyc:MENF-CPLX		has_cofactor

magnesium(2+)
CHEBI:18420
  • UniProtKB:P38051

    Source assertion: The reviewed entry specifies CHEBI:18420 with ECO:0000255 HAMAP-Rule:MF_01935, ECO:0000269 PubMed:17240978, ECO:0000269 PubMed:18453696, ECO:0000269 PubMed:9150206, ECO:0000269 PubMed:9795253. Binds more tightly in the presence of chorismate.

    Source location/comments/2/cofactors; /comments/2/note
chorismate => isochorismate
RHEA:18986		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes PWY-5837 as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:PWY-5837

    Source assertion: The full native subpathway includes ISOCHORSYN-RXN and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-5837"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="ISOCHORSYN-RXN"]/direction
  • MetaCyc:ISOCHORSYN-RXN

    Source assertion: The native reaction explicitly cross-references RHEA:18988.

    Source locationReaction[@frameid="ISOCHORSYN-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:18986

    Source assertion: RHEA:18985 links native cited member RHEA:18988 and selected left-to-right member RHEA:18986.

    Source locationhttp://rdf.rhea-db.org/18985 rh:bidirectionalReaction http://rdf.rhea-db.org/18988; http://rdf.rhea-db.org/18985 rh:directionalReaction http://rdf.rhea-db.org/18986; http://rdf.rhea-db.org/18986 rh:substrates; rh:products
  • MetaCyc:PWY-5838

    Source assertion: The native superpathway species list includes MG1655, a K-12 substrain.

    Source locationPathway[@frameid="PWY-5838"]/species/Organism[@frameid="TAX-511145"]
  • UniProtKB:P38051

    Source assertion: The chosen reviewed protein entry is taxon 83333 (E. coli K-12). Other selected reviewed proteins share this taxon.

    Source location/organism/taxonId; /organism/scientificName
  • PMID:23564174

    Source assertion: The study uses MG1655 genes for recombinant thioesterase work and BW25113-derived deletions for quinone measurements; this is specific experimental support within K-12, not evidence for identical flux in every derivative.

    Source locationMaterials and Methods, Expression and purification of proteins (#sec4); Results and Discussion, Effect of the ydiI knockout on production of menaquinone (#sec11).
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
MetaCyc:CPLX0-7525		catalyzes

isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594
  • UniProtKB:P17109

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000255 HAMAP-Rule:MF_01659, ECO:0000269 PubMed:17760421. Its Rhea reaction is RHEA:25593.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:25594

    Source assertion: RHEA:25593 explicitly links the left-to-right directional reaction RHEA:25594.

    Source locationhttp://rdf.rhea-db.org/25593 rh:directionalReaction http://rdf.rhea-db.org/25594; http://rdf.rhea-db.org/25594 rh:substrates; rh:products; rh:equation
  • MetaCyc:CPLX0-7525

    Source assertion: Native CPLX0-7525 explicitly links its catalyzed enzymatic reaction to 2.5.1.64-RXN; this supports the reaction assignment.

    Source locationProtein[@frameid="CPLX0-7525"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:17760421

    Source assertion: The primary abstract identifies SEPHCHC as the actual E. coli MenD product and distinguishes subsequent pH-dependent spontaneous SHCHC formation from the enzyme reaction.

    Source location/resultList/result[id="17760421"]/abstractText
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
UniProtKB:P17109		part_of

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
MetaCyc:CPLX0-7525
  • MetaCyc:CPLX0-7525

    Source assertion: The native MG1655 complex has 2 copies of MEND-MONOMER.

    Source locationProtein[@frameid="CPLX0-7525"]/component/Protein/@frameid; component/coefficient; species/Organism/@frameid
  • MetaCyc:MEND-MONOMER

    Source assertion: The native protein explicitly unifies with UniProt P17109.

    Source locationProtein[@frameid="MEND-MONOMER"]/dblink[dblink-db="UNIPROT"]/dblink-oid; dblink-relationship
  • UniProtKB:P17109

    Source assertion: SUBUNIT states Homodimer; its evidence codes remain explicit in the original reviewed entry.

    Source location/comments/7/texts
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
MetaCyc:CPLX0-7525		has_cofactor

Metal alternatives do not imply simultaneous magnesium and manganese occupancy.

magnesium(2+)
CHEBI:18420
  • UniProtKB:P17109

    Source assertion: The reviewed entry specifies CHEBI:18420 with ECO:0000255 HAMAP-Rule:MF_01659.

    Source location/comments/2/cofactors
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
MetaCyc:CPLX0-7525		has_cofactor

Metal alternatives do not imply simultaneous magnesium and manganese occupancy.

manganese(2+)
CHEBI:29035
  • UniProtKB:P17109

    Source assertion: The reviewed entry specifies CHEBI:29035 with ECO:0000255 HAMAP-Rule:MF_01659.

    Source location/comments/2/cofactors
  • PMID:14621995

    Source assertion: The primary abstract describes a dimeric thiamine-diphosphate-dependent E. coli MenD that prefers manganese. Its obsolete SHCHC product assignment is not adopted.

    Source location/resultList/result[id="14621995"]/abstractText
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
MetaCyc:CPLX0-7525		has_cofactor

thiamine(1+) diphosphate(3-)
CHEBI:58937
  • UniProtKB:P17109

    Source assertion: The reviewed entry specifies CHEBI:58937 with ECO:0000255 HAMAP-Rule:MF_01659. Binds 1 thiamine pyrophosphate per subunit.

    Source location/comments/3/cofactors; /comments/3/note
  • PMID:14621995

    Source assertion: The primary abstract describes a dimeric thiamine-diphosphate-dependent E. coli MenD that prefers manganese. Its obsolete SHCHC product assignment is not adopted.

    Source location/resultList/result[id="14621995"]/abstractText
isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes PWY-5837 as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:PWY-5837

    Source assertion: The full native subpathway includes 2.5.1.64-RXN and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-5837"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="2.5.1.64-RXN"]/direction
  • MetaCyc:2.5.1.64-RXN

    Source assertion: The native reaction explicitly cross-references RHEA:25594.

    Source locationReaction[@frameid="2.5.1.64-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:25594

    Source assertion: RHEA:25593 explicitly links the left-to-right directional reaction RHEA:25594.

    Source locationhttp://rdf.rhea-db.org/25593 rh:directionalReaction http://rdf.rhea-db.org/25594; http://rdf.rhea-db.org/25594 rh:substrates; rh:products; rh:equation
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
UniProtKB:P37355		catalyzes

Monomeric MenH catalyzes the SHCHC-forming elimination; it is not assigned the later DHNA-CoA hydrolysis.

5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate
RHEA:25598
  • UniProtKB:P37355

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000255 HAMAP-Rule:MF_01660, ECO:0000269 PubMed:18284213. Its Rhea reaction is RHEA:25597.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:25598

    Source assertion: RHEA:25597 explicitly links the left-to-right directional reaction RHEA:25598.

    Source locationhttp://rdf.rhea-db.org/25597 rh:directionalReaction http://rdf.rhea-db.org/25598; http://rdf.rhea-db.org/25598 rh:substrates; rh:products; rh:equation
  • MetaCyc:EG12438-MONOMER

    Source assertion: Native EG12438-MONOMER explicitly links its catalyzed enzymatic reaction to RXN-9310; this supports the reaction assignment.

    Source locationProtein[@frameid="EG12438-MONOMER"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:18284213

    Source assertion: The primary abstract identifies E. coli MenH as SHCHC synthase, catalyzing 2,5-elimination of pyruvate from SEPHCHC; it rejects the former thioesterase assignment.

    Source location/resultList/result[id="18284213"]/abstractText
  • PMID:23637813

    Source assertion: Purified tagged EcMenH elutes as a monomer by size exclusion; dynamic light scattering and crystal-interface analysis support monomeric solution behavior.

    Source locationMethods, Cloning, Expression and Purification (#s2a, second paragraph); Results, Overview of the Crystal Structures (#s3a, first paragraph).
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate
RHEA:25598		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes PWY-5837 as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:PWY-5837

    Source assertion: The full native subpathway includes RXN-9310 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-5837"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="RXN-9310"]/direction
  • MetaCyc:RXN-9310

    Source assertion: The native reaction explicitly cross-references RHEA:25598.

    Source locationReaction[@frameid="RXN-9310"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:25598

    Source assertion: RHEA:25597 explicitly links the left-to-right directional reaction RHEA:25598.

    Source locationhttp://rdf.rhea-db.org/25597 rh:directionalReaction http://rdf.rhea-db.org/25598; http://rdf.rhea-db.org/25598 rh:substrates; rh:products; rh:equation
o-succinylbenzoate synthase
UniProtKB:P29208		enables

Functional activity is represented without asserting isolated-monomer sufficiency.

(1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate => 2-succinylbenzoate + H2O
RHEA:10197
  • UniProtKB:P29208

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000255 HAMAP-Rule:MF_00470, ECO:0000269 PubMed:10194342, ECO:0000269 PubMed:8335646. Its Rhea reaction is RHEA:10196.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:10197

    Source assertion: RHEA:10196 explicitly links the left-to-right directional reaction RHEA:10197.

    Source locationhttp://rdf.rhea-db.org/10196 rh:directionalReaction http://rdf.rhea-db.org/10197; http://rdf.rhea-db.org/10197 rh:substrates; rh:products; rh:equation
  • MetaCyc:O-SUCCINYLBENZOATE-COA-SYN-MONOMER

    Source assertion: Native O-SUCCINYLBENZOATE-COA-SYN-MONOMER explicitly links its catalyzed enzymatic reaction to O-SUCCINYLBENZOATE-COA-SYN-RXN; this supports the reaction assignment.

    Source locationProtein[@frameid="O-SUCCINYLBENZOATE-COA-SYN-MONOMER"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:10978150

    Source assertion: The primary abstract describes E. coli o-succinylbenzoate synthase and its Mg(2+)-OSB complex, with acid/base residues positioned for the dehydration reaction.

    Source location/resultList/result[id="10978150"]/abstractText
o-succinylbenzoate synthase
UniProtKB:P29208		has_cofactor

magnesium(2+)
CHEBI:18420
  • UniProtKB:P29208

    Source assertion: The reviewed entry specifies CHEBI:18420 with ECO:0000269 PubMed:10978150.

    Source location/comments/2/cofactors
(1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate => 2-succinylbenzoate + H2O
RHEA:10197		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes PWY-5837 as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:PWY-5837

    Source assertion: The full native subpathway includes O-SUCCINYLBENZOATE-COA-SYN-RXN and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-5837"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="O-SUCCINYLBENZOATE-COA-SYN-RXN"]/direction
  • MetaCyc:O-SUCCINYLBENZOATE-COA-SYN-RXN

    Source assertion: The native reaction explicitly cross-references RHEA:10197.

    Source locationReaction[@frameid="O-SUCCINYLBENZOATE-COA-SYN-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:10197

    Source assertion: RHEA:10196 explicitly links the left-to-right directional reaction RHEA:10197.

    Source locationhttp://rdf.rhea-db.org/10196 rh:directionalReaction http://rdf.rhea-db.org/10197; http://rdf.rhea-db.org/10197 rh:substrates; rh:products; rh:equation
2-succinylbenzoate--CoA ligase
UniProtKB:P37353		enables

Supported MenE function; native tetramer and reviewed homodimer-by-similarity assignments conflict, while inhibitor-bound author/PISA structures are monomeric. No unique oligomer is asserted.

2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010
  • UniProtKB:P37353

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000255 HAMAP-Rule:MF_00731, ECO:0000269 PubMed:22109989, ECO:0000269 PubMed:26394156. Its Rhea reaction is RHEA:17009.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:17010

    Source assertion: RHEA:17009 explicitly links the left-to-right directional reaction RHEA:17010.

    Source locationhttp://rdf.rhea-db.org/17009 rh:directionalReaction http://rdf.rhea-db.org/17010; http://rdf.rhea-db.org/17010 rh:substrates; rh:products; rh:equation
  • MetaCyc:MENE-CPLX

    Source assertion: Native MENE-CPLX explicitly links its catalyzed enzymatic reaction to O-SUCCINYLBENZOATE-COA-LIG-RXN; this supports the reaction assignment.

    Source locationProtein[@frameid="MENE-CPLX"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:26394156

    Source assertion: Purified E. coli MenE is assayed with OSB, ATP and CoA in a MenB-coupled reaction; MgCl2 is present. This establishes activity, not a unique physiological oligomer.

    Source locationMaterials and Methods, Enzyme assays (#P10); Results and Discussion, Role of R195 in OSB-CoA formation and inhibition of MenE; Table 2 (#P39).
  • MetaCyc:MENE-CPLX

    Source assertion: The native complex has four copies of O-SUCCINYLBENZOATE-COA-LIG-MONOMER; this conflicts with the current reviewed assembly annotation and is not installed as a resolved physical assembly.

    Source locationProtein[@frameid="MENE-CPLX"]/component/Protein; component/coefficient
  • UniProtKB:P37353

    Source assertion: SUBUNIT assigns Homodimer by similarity (ECO:0000250 UniProtKB:P23971).

    Source location/comments/6/texts
  • DOI:10.2210/pdb6NJ0/pdb

    Source assertion: For PDB 6NJ0, author- and PISA-defined biological assembly 1 is monomeric. Protein, variant and ligand identities are separately grounded in the cited primary article; this assembly does not resolve all solution or catalytic conditions.

    Source location/pdbx_struct_assembly/oligomeric_count; /pdbx_struct_assembly/oligomeric_details; /pdbx_struct_assembly/details; /pdbx_struct_assembly/method_details; /rcsb_struct_symmetry
  • DOI:10.2210/pdb5C5H/pdb

    Source assertion: For PDB 5C5H, author- and PISA-defined biological assembly 1 is monomeric. Protein, variant and ligand identities are separately grounded in the cited primary article; this assembly does not resolve all solution or catalytic conditions.

    Source location/pdbx_struct_assembly/oligomeric_count; /pdbx_struct_assembly/oligomeric_details; /pdbx_struct_assembly/details; /pdbx_struct_assembly/method_details; /rcsb_struct_symmetry
  • PMID:30912442

    Source assertion: The primary Methods and Results identify PDB 6NJ0 as wild-type E. coli MenE co-crystallized with m-phenyl ether inhibitor analogue 5. The structure used R195K E. coli MenE bound to OSB-AMS (5C5H) as a molecular-replacement model. These are inhibitor-bound structures, not evidence resolving all physiological assemblies.

    Source locationPMC6653581 original HTML, Methods paragraph #P17; Results paragraph #P32.
  • PMID:26394156

    Source assertion: The primary Results identify the R195K E. coli MenE mutant co-crystallized with OSB-AMS as PDB 5C5H; Methods give the crystallization conditions. This grounds the variant and ligand context separately from the RCSB assembly annotation.

    Source locationPMC4624480 original HTML, Methods paragraph #P11; Results paragraph #P23.
2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes PWY-5837 as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:PWY-5837

    Source assertion: The full native subpathway includes O-SUCCINYLBENZOATE-COA-LIG-RXN and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-5837"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="O-SUCCINYLBENZOATE-COA-LIG-RXN"]/direction
  • MetaCyc:O-SUCCINYLBENZOATE-COA-LIG-RXN

    Source assertion: The native reaction explicitly cross-references RHEA:17010.

    Source locationReaction[@frameid="O-SUCCINYLBENZOATE-COA-LIG-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:17010

    Source assertion: RHEA:17009 explicitly links the left-to-right directional reaction RHEA:17010.

    Source locationhttp://rdf.rhea-db.org/17009 rh:directionalReaction http://rdf.rhea-db.org/17010; http://rdf.rhea-db.org/17010 rh:substrates; rh:products; rh:equation
1,4-dihydroxy-2-naphthoyl-CoA synthase
MetaCyc:CPLX0-7882		catalyzes

2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O
RHEA:26563
  • UniProtKB:P0ABU0

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000255 HAMAP-Rule:MF_01934, ECO:0000269 PubMed:20643650, ECO:0000269 PubMed:21830810, ECO:0000269 PubMed:22606952, ECO:0000269 PubMed:23658663. Its Rhea reaction is RHEA:26562.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:26563

    Source assertion: RHEA:26562 explicitly links the left-to-right directional reaction RHEA:26563.

    Source locationhttp://rdf.rhea-db.org/26562 rh:directionalReaction http://rdf.rhea-db.org/26563; http://rdf.rhea-db.org/26563 rh:substrates; rh:products; rh:equation
  • MetaCyc:CPLX0-7882

    Source assertion: Native CPLX0-7882 explicitly links its catalyzed enzymatic reaction to NAPHTHOATE-SYN-RXN; this supports the reaction assignment.

    Source locationProtein[@frameid="CPLX0-7882"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:20643650

    Source assertion: Untagged E. coli XL1 Blue MenB forms DHNA-CoA and is hexameric in solution; bicarbonate activates the enzyme. The tagged enzyme is inactive and no DHNA synthase activity was detected.

    Source locationExperimental Procedures, Expression and Purification of Enzymes (#sec6); Results, Bicarbonate Activation of the EcMenB Activity (#sec15, first paragraph).
1,4-dihydroxy-2-naphthoyl-CoA synthase
UniProtKB:P0ABU0		part_of

1,4-dihydroxy-2-naphthoyl-CoA synthase
MetaCyc:CPLX0-7882
  • MetaCyc:CPLX0-7882

    Source assertion: The native MG1655 complex has 6 copies of NAPHTHOATE-SYN-MONOMER.

    Source locationProtein[@frameid="CPLX0-7882"]/component/Protein/@frameid; component/coefficient; species/Organism/@frameid
  • MetaCyc:NAPHTHOATE-SYN-MONOMER

    Source assertion: The native protein explicitly unifies with UniProt P0ABU0.

    Source locationProtein[@frameid="NAPHTHOATE-SYN-MONOMER"]/dblink[dblink-db="UNIPROT"]/dblink-oid; dblink-relationship
  • UniProtKB:P0ABU0

    Source assertion: SUBUNIT states Homohexamer. Dimer of a homotrimer; its evidence codes remain explicit in the original reviewed entry.

    Source location/comments/7/texts
1,4-dihydroxy-2-naphthoyl-CoA synthase
MetaCyc:CPLX0-7882		has_cofactor

hydrogencarbonate
CHEBI:17544
  • UniProtKB:P0ABU0

    Source assertion: The reviewed entry specifies CHEBI:17544 with ECO:0000255 HAMAP-Rule:MF_01934, ECO:0000269 PubMed:20643650, ECO:0000305 PubMed:22606952. The hydrogencarbonate anion plays the same catalytic role (proton acceptor) as the side-chain carboxylate group of the essential 'Asp-185' found in actinobacteria, archaea, bacteroidetes, and deltaproteobacteria.

    Source location/comments/2/cofactors; /comments/2/note
  • PMID:20643650

    Source assertion: Purified untagged EcMenB activity depends on exogenous bicarbonate; the structural binding-site comparison used the closely related Salmonella enzyme.

    Source locationResults, Bicarbonate Activation of the EcMenB Activity (#sec15) and Active Site and Bicarbonate Binding Site in EcMenB/StMenB; Discussion.
2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O
RHEA:26563		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes PWY-5837 as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:PWY-5837

    Source assertion: The full native subpathway includes NAPHTHOATE-SYN-RXN and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-5837"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="NAPHTHOATE-SYN-RXN"]/direction
  • MetaCyc:NAPHTHOATE-SYN-RXN

    Source assertion: The native reaction explicitly cross-references RHEA:26563.

    Source locationReaction[@frameid="NAPHTHOATE-SYN-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:26563

    Source assertion: RHEA:26562 explicitly links the left-to-right directional reaction RHEA:26563.

    Source locationhttp://rdf.rhea-db.org/26562 rh:directionalReaction http://rdf.rhea-db.org/26563; http://rdf.rhea-db.org/26563 rh:substrates; rh:products; rh:equation
1,4-dihydroxy-2-naphthoyl-CoA hydrolase
MetaCyc:CPLX0-8128		catalyzes

1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310
  • UniProtKB:P77781

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000255 HAMAP-Rule:MF_01936, ECO:0000269 PubMed:23564174, ECO:0000269 PubMed:24992697. Its Rhea reaction is RHEA:26309.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:26310

    Source assertion: RHEA:26309 explicitly links the left-to-right directional reaction RHEA:26310.

    Source locationhttp://rdf.rhea-db.org/26309 rh:directionalReaction http://rdf.rhea-db.org/26310; http://rdf.rhea-db.org/26310 rh:substrates; rh:products; rh:equation
  • MetaCyc:CPLX0-8128

    Source assertion: Native CPLX0-8128 explicitly links its catalyzed enzymatic reaction to RXN-9311; this supports the reaction assignment.

    Source locationProtein[@frameid="CPLX0-8128"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:23564174

    Source assertion: MG1655-derived recombinant YdiI hydrolyzes DHNA-CoA. BW25113 deletion mutants have reduced menaquinone production; residual production prevents an absolute indispensability claim.

    Source locationMaterials and Methods, Expression and purification of proteins (#sec4); Results and Discussion, Effect of the ydiI knockout on production of menaquinone (#sec11).
1,4-dihydroxy-2-naphthoyl-CoA hydrolase
UniProtKB:P77781		part_of

1,4-dihydroxy-2-naphthoyl-CoA hydrolase
MetaCyc:CPLX0-8128
  • MetaCyc:CPLX0-8128

    Source assertion: The native MG1655 complex has 4 copies of G6912-MONOMER.

    Source locationProtein[@frameid="CPLX0-8128"]/component/Protein/@frameid; component/coefficient; species/Organism/@frameid
  • MetaCyc:G6912-MONOMER

    Source assertion: The native protein explicitly unifies with UniProt P77781.

    Source locationProtein[@frameid="G6912-MONOMER"]/dblink[dblink-db="UNIPROT"]/dblink-oid; dblink-relationship
  • UniProtKB:P77781

    Source assertion: SUBUNIT states Homotetramer. Dimer of dimers; its evidence codes remain explicit in the original reviewed entry.

    Source location/comments/5/texts
  • PMID:25010423

    Source assertion: The YdiI quaternary structure is a dimer of dimers with active sites spanning subunits.

    Source locationResults and Discussion, Structural Determinants of YbdB and YdiI Substrate Specificity and Catalysis (sec3.1, second paragraph); Figure 1B-D.
1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes PWY-5837 as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:PWY-5837

    Source assertion: The full native subpathway includes RXN-9311 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-5837"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="RXN-9311"]/direction
  • MetaCyc:RXN-9311

    Source assertion: The native reaction explicitly cross-references RHEA:26310.

    Source locationReaction[@frameid="RXN-9311"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:26310

    Source assertion: RHEA:26309 explicitly links the left-to-right directional reaction RHEA:26310.

    Source locationhttp://rdf.rhea-db.org/26309 rh:directionalReaction http://rdf.rhea-db.org/26310; http://rdf.rhea-db.org/26310 rh:substrates; rh:products; rh:equation
all-trans-octaprenyl-diphosphate synthase
MetaCyc:CPLX0-7426		catalyzes

5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate
RHEA:27799
  • UniProtKB:P0AD57

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000269 PubMed:8037730. Its Rhea reaction is RHEA:27798.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:27799

    Source assertion: RHEA:27798 explicitly links the left-to-right directional reaction RHEA:27799.

    Source locationhttp://rdf.rhea-db.org/27798 rh:directionalReaction http://rdf.rhea-db.org/27799; http://rdf.rhea-db.org/27799 rh:substrates; rh:products; rh:equation
  • MetaCyc:CPLX0-7426

    Source assertion: Native CPLX0-7426 explicitly links its catalyzed enzymatic reaction to RXN-8992; this supports the reaction assignment.

    Source locationProtein[@frameid="CPLX0-7426"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:8037730

    Source assertion: The primary abstract assigns E. coli ispB by increased prenyltransferase activity and product analysis identifying the phosphate ester of all-E-octaprenol.

    Source location/resultList/result[id="8037730"]/abstractText
Octaprenyl diphosphate synthase
UniProtKB:P0AD57		part_of

all-trans-octaprenyl-diphosphate synthase
MetaCyc:CPLX0-7426
  • MetaCyc:CPLX0-7426

    Source assertion: The native MG1655 complex has 2 copies of OPPSYN-MONOMER.

    Source locationProtein[@frameid="CPLX0-7426"]/component/Protein/@frameid; component/coefficient; species/Organism/@frameid
  • MetaCyc:OPPSYN-MONOMER

    Source assertion: The native protein explicitly unifies with UniProt P0AD57.

    Source locationProtein[@frameid="OPPSYN-MONOMER"]/dblink[dblink-db="UNIPROT"]/dblink-oid; dblink-relationship
  • PMID:11108713

    Source assertion: The primary abstract demonstrates IspB dimer formation and altered chain length in a tagged wild-type/mutant heterodimer; this corroborates dimerization without assigning the mutant phenotype to the wild type.

    Source location/resultList/result[id="11108713"]/abstractText
all-trans-octaprenyl-diphosphate synthase
MetaCyc:CPLX0-7426		has_cofactor

The IspB magnesium assignment and two ions per subunit are by similarity.

magnesium(2+)
CHEBI:18420
  • UniProtKB:P0AD57

    Source assertion: The reviewed entry specifies CHEBI:18420 with ECO:0000250 :. Binds 2 Mg(2+) ions per subunit.

    Source location/comments/2/cofactors; /comments/2/note
5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate
RHEA:27799		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes PWY-5783 as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:PWY-5783

    Source assertion: The full native subpathway includes RXN-8992 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-5783"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="RXN-8992"]/direction
  • MetaCyc:RXN-8992

    Source assertion: The native reaction explicitly cross-references RHEA:27799.

    Source locationReaction[@frameid="RXN-8992"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:27799

    Source assertion: RHEA:27798 explicitly links the left-to-right directional reaction RHEA:27799.

    Source locationhttp://rdf.rhea-db.org/27798 rh:directionalReaction http://rdf.rhea-db.org/27799; http://rdf.rhea-db.org/27799 rh:substrates; rh:products; rh:equation
1,4-dihydroxy-2-naphthoate octaprenyltransferase
UniProtKB:P32166		enables

Functional activity is represented without asserting isolated-monomer sufficiency.

all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100
  • UniProtKB:P32166

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000255 HAMAP-Rule:MF_01937, ECO:0000269 PubMed:9573170. Its Rhea reaction is RHEA:26478.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:30100

    Source assertion: RHEA:30099 explicitly links the left-to-right directional reaction RHEA:30100.

    Source locationhttp://rdf.rhea-db.org/30099 rh:directionalReaction http://rdf.rhea-db.org/30100; http://rdf.rhea-db.org/30100 rh:substrates; rh:products; rh:equation
  • RHEA:30100

    Source assertion: Exact octaprenyl master RHEA:30099 is explicitly rdfs:subClassOf generic RHEA:26478; the selected native reaction fixes the eight-unit chain.

    Source locationhttp://rdf.rhea-db.org/30099 rdfs:subClassOf http://rdf.rhea-db.org/26478; http://rdf.rhea-db.org/30099 rh:directionalReaction http://rdf.rhea-db.org/30100
  • MetaCyc:DMK-MONOMER

    Source assertion: Native DMK-MONOMER explicitly links its catalyzed enzymatic reaction to DMK-RXN; this supports the reaction assignment.

    Source locationProtein[@frameid="DMK-MONOMER"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:9573170

    Source assertion: K-12 menA mutant complementation restores MK-8/DMK-8 and membrane prenyltransferase activity. The assay used farnesyl diphosphate; natural octaprenyl use is discussed from prior work. Oxidation to the extracted quinone is described as thought spontaneous, not a demonstrated additional biosynthetic enzyme.

    Source locationBacterial strains and growth conditions; Isolation of membranes and assay of DHNA octaprenyltransferase (#sec6); Analysis of menaquinones and octaprenyltransferase activity in various strains; introductory paragraph beginning The conversion of DHNA to DMK, and Figure 1.
all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes PWY-5852 as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:PWY-5852

    Source assertion: The full native subpathway includes DMK-RXN and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-5852"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="DMK-RXN"]/direction
  • MetaCyc:DMK-RXN

    Source assertion: The native reaction explicitly cross-references RHEA:30100.

    Source locationReaction[@frameid="DMK-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:30100

    Source assertion: RHEA:30099 explicitly links the left-to-right directional reaction RHEA:30100.

    Source locationhttp://rdf.rhea-db.org/30099 rh:directionalReaction http://rdf.rhea-db.org/30100; http://rdf.rhea-db.org/30100 rh:substrates; rh:products; rh:equation
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE
UniProtKB:P0A887		enables

Fixed-chain menaquinol methylation follows native and Rhea chemistry. The ubiquinone Ubi metabolon is not asserted as its required assembly here.

2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+)
RHEA:30064
  • UniProtKB:P0A887

    Source assertion: The reviewed K-12 entry assigns this catalytic function with ECO:0000255 HAMAP-Rule:MF_01813, ECO:0000269 PubMed:9045837. Its Rhea reaction is RHEA:42640.

    Source location/organism/taxonId; /comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences; /comments/1/reaction/evidences
  • RHEA:30064

    Source assertion: RHEA:30063 explicitly links the left-to-right directional reaction RHEA:30064.

    Source locationhttp://rdf.rhea-db.org/30063 rh:directionalReaction http://rdf.rhea-db.org/30064; http://rdf.rhea-db.org/30064 rh:substrates; rh:products; rh:equation
  • RHEA:30064

    Source assertion: Exact octaprenyl master RHEA:30063 is explicitly rdfs:subClassOf generic RHEA:42640; the selected native reaction fixes the eight-unit chain.

    Source locationhttp://rdf.rhea-db.org/30063 rdfs:subClassOf http://rdf.rhea-db.org/42640; http://rdf.rhea-db.org/30063 rh:directionalReaction http://rdf.rhea-db.org/30064
  • MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER

    Source assertion: Native 2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER explicitly links its catalyzed enzymatic reaction to ADOMET-DMK-METHYLTRANSFER-RXN; this supports the reaction assignment.

    Source locationProtein[@frameid="2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER"]/catalyzes/Enzymatic-Reaction/reaction/Reaction/@frameid
  • PMID:9045837

    Source assertion: The primary abstract identifies UbiE through complementation, disruption and accumulated demethylmenaquinone. It supports C-methylation in both quinone pathways, without proving the redox state of every intracellular substrate.

    Source location/resultList/result[id="9045837"]/abstractText
2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+)
RHEA:30064		part_of

MetaCyc:PWY-5838
  • MetaCyc:PWY-5838

    Source assertion: The complete superpathway explicitly includes MENAQUINONESYN-PWY as a pathway member.

    Source locationPathway[@frameid="PWY-5838"]/reaction-list/Pathway/@frameid
  • MetaCyc:MENAQUINONESYN-PWY

    Source assertion: The full native subpathway includes ADOMET-DMK-METHYLTRANSFER-RXN and selects L2R in its reaction layout.

    Source locationPathway[@frameid="MENAQUINONESYN-PWY"]/reaction-list/Reaction/@frameid; reaction-layout[Reaction/@frameid="ADOMET-DMK-METHYLTRANSFER-RXN"]/direction
  • MetaCyc:ADOMET-DMK-METHYLTRANSFER-RXN

    Source assertion: The native reaction explicitly cross-references RHEA:30064.

    Source locationReaction[@frameid="ADOMET-DMK-METHYLTRANSFER-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:30064

    Source assertion: RHEA:30063 explicitly links the left-to-right directional reaction RHEA:30064.

    Source locationhttp://rdf.rhea-db.org/30063 rh:directionalReaction http://rdf.rhea-db.org/30064; http://rdf.rhea-db.org/30064 rh:substrates; rh:products; rh:equation
1,4-dihydroxy-2-naphthoate octaprenyltransferase
UniProtKB:P32166		located_in

plasma membrane
GO:0005886
  • UniProtKB:P32166

    Source assertion: SUBCELLULAR LOCATION assigns MenA to the cell inner membrane as a multipass protein, with experimental PMID:15919996 and HAMAP support.

    Source location/comments/3/subcellularLocations
chorismate => isochorismate
RHEA:18986		provides_input_for

Curated continuity through CHEBI:29780; no physical channeling or flux magnitude is asserted.

isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594
  • RHEA:18986

    Source assertion: RHEA:18986 explicitly has shared intermediate CHEBI:29780 in its products.

    Source locationhttp://rdf.rhea-db.org/18986 rh:products http://rdf.rhea-db.org/18985_R; http://rdf.rhea-db.org/18985_R rh:contains1 http://rdf.rhea-db.org/Participant_18985_compound_3577; http://rdf.rhea-db.org/Participant_18985_compound_3577 rh:compound http://rdf.rhea-db.org/Compound_3577; http://rdf.rhea-db.org/Compound_3577 rh:accession
  • RHEA:25594

    Source assertion: RHEA:25594 explicitly has shared intermediate CHEBI:29780 in its substrates.

    Source locationhttp://rdf.rhea-db.org/25594 rh:substrates http://rdf.rhea-db.org/25593_L; http://rdf.rhea-db.org/25593_L rh:contains1 http://rdf.rhea-db.org/Participant_25593_compound_3577; http://rdf.rhea-db.org/Participant_25593_compound_3577 rh:compound http://rdf.rhea-db.org/Compound_3577; http://rdf.rhea-db.org/Compound_3577 rh:accession
isochorismate + 2-oxoglutarate + H(+) => 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
RHEA:25594		provides_input_for

Curated continuity through CHEBI:58818; no physical channeling or flux magnitude is asserted.

5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate
RHEA:25598
  • RHEA:25594

    Source assertion: RHEA:25594 explicitly has shared intermediate CHEBI:58818 in its products.

    Source locationhttp://rdf.rhea-db.org/25594 rh:products http://rdf.rhea-db.org/25593_R; http://rdf.rhea-db.org/25593_R rh:contains1 http://rdf.rhea-db.org/Participant_25593_compound_5683; http://rdf.rhea-db.org/Participant_25593_compound_5683 rh:compound http://rdf.rhea-db.org/Compound_5683; http://rdf.rhea-db.org/Compound_5683 rh:accession
  • RHEA:25598

    Source assertion: RHEA:25598 explicitly has shared intermediate CHEBI:58818 in its substrates.

    Source locationhttp://rdf.rhea-db.org/25598 rh:substrates http://rdf.rhea-db.org/25597_L; http://rdf.rhea-db.org/25597_L rh:contains1 http://rdf.rhea-db.org/Participant_25597_compound_5683; http://rdf.rhea-db.org/Participant_25597_compound_5683 rh:compound http://rdf.rhea-db.org/Compound_5683; http://rdf.rhea-db.org/Compound_5683 rh:accession
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate => (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate
RHEA:25598		provides_input_for

Curated continuity through CHEBI:58689; no physical channeling or flux magnitude is asserted.

(1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate => 2-succinylbenzoate + H2O
RHEA:10197
  • RHEA:25598

    Source assertion: RHEA:25598 explicitly has shared intermediate CHEBI:58689 in its products.

    Source locationhttp://rdf.rhea-db.org/25598 rh:products http://rdf.rhea-db.org/25597_R; http://rdf.rhea-db.org/25597_R rh:contains1 http://rdf.rhea-db.org/Participant_25597_compound_5418; http://rdf.rhea-db.org/Participant_25597_compound_5418 rh:compound http://rdf.rhea-db.org/Compound_5418; http://rdf.rhea-db.org/Compound_5418 rh:accession
  • RHEA:10197

    Source assertion: RHEA:10197 explicitly has shared intermediate CHEBI:58689 in its substrates.

    Source locationhttp://rdf.rhea-db.org/10197 rh:substrates http://rdf.rhea-db.org/10196_L; http://rdf.rhea-db.org/10196_L rh:contains1 http://rdf.rhea-db.org/Participant_10196_compound_5418; http://rdf.rhea-db.org/Participant_10196_compound_5418 rh:compound http://rdf.rhea-db.org/Compound_5418; http://rdf.rhea-db.org/Compound_5418 rh:accession
(1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate => 2-succinylbenzoate + H2O
RHEA:10197		provides_input_for

Curated continuity through CHEBI:18325; no physical channeling or flux magnitude is asserted.

2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010
  • RHEA:10197

    Source assertion: RHEA:10197 explicitly has shared intermediate CHEBI:18325 in its products.

    Source locationhttp://rdf.rhea-db.org/10197 rh:products http://rdf.rhea-db.org/10196_R; http://rdf.rhea-db.org/10196_R rh:contains1 http://rdf.rhea-db.org/Participant_10196_compound_5241; http://rdf.rhea-db.org/Participant_10196_compound_5241 rh:compound http://rdf.rhea-db.org/Compound_5241; http://rdf.rhea-db.org/Compound_5241 rh:accession
  • RHEA:17010

    Source assertion: RHEA:17010 explicitly has shared intermediate CHEBI:18325 in its substrates.

    Source locationhttp://rdf.rhea-db.org/17010 rh:substrates http://rdf.rhea-db.org/17009_L; http://rdf.rhea-db.org/17009_L rh:contains1 http://rdf.rhea-db.org/Participant_17009_compound_5241; http://rdf.rhea-db.org/Participant_17009_compound_5241 rh:compound http://rdf.rhea-db.org/Compound_5241; http://rdf.rhea-db.org/Compound_5241 rh:accession
2-succinylbenzoate + ATP + CoA => 2-succinylbenzoyl-CoA + AMP + diphosphate
RHEA:17010		provides_input_for

Curated continuity through CHEBI:57364; no physical channeling or flux magnitude is asserted.

2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O
RHEA:26563
  • RHEA:17010

    Source assertion: RHEA:17010 explicitly has shared intermediate CHEBI:57364 in its products.

    Source locationhttp://rdf.rhea-db.org/17010 rh:products http://rdf.rhea-db.org/17009_R; http://rdf.rhea-db.org/17009_R rh:contains1 http://rdf.rhea-db.org/Participant_17009_compound_2208; http://rdf.rhea-db.org/Participant_17009_compound_2208 rh:compound http://rdf.rhea-db.org/Compound_2208; http://rdf.rhea-db.org/Compound_2208 rh:accession
  • RHEA:26563

    Source assertion: RHEA:26563 explicitly has shared intermediate CHEBI:57364 in its substrates.

    Source locationhttp://rdf.rhea-db.org/26563 rh:substrates http://rdf.rhea-db.org/26562_L; http://rdf.rhea-db.org/26562_L rh:contains1 http://rdf.rhea-db.org/Participant_26562_compound_2208; http://rdf.rhea-db.org/Participant_26562_compound_2208 rh:compound http://rdf.rhea-db.org/Compound_2208; http://rdf.rhea-db.org/Compound_2208 rh:accession
2-succinylbenzoyl-CoA + H(+) => 1,4-dihydroxy-2-naphthoyl-CoA + H2O
RHEA:26563		provides_input_for

Curated continuity through CHEBI:58897; no physical channeling or flux magnitude is asserted.

1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310
  • RHEA:26563

    Source assertion: RHEA:26563 explicitly has shared intermediate CHEBI:58897 in its products.

    Source locationhttp://rdf.rhea-db.org/26563 rh:products http://rdf.rhea-db.org/26562_R; http://rdf.rhea-db.org/26562_R rh:contains1 http://rdf.rhea-db.org/Participant_26562_compound_5962; http://rdf.rhea-db.org/Participant_26562_compound_5962 rh:compound http://rdf.rhea-db.org/Compound_5962; http://rdf.rhea-db.org/Compound_5962 rh:accession
  • RHEA:26310

    Source assertion: RHEA:26310 explicitly has shared intermediate CHEBI:58897 in its substrates.

    Source locationhttp://rdf.rhea-db.org/26310 rh:substrates http://rdf.rhea-db.org/26309_L; http://rdf.rhea-db.org/26309_L rh:contains1 http://rdf.rhea-db.org/Participant_26309_compound_5962; http://rdf.rhea-db.org/Participant_26309_compound_5962 rh:compound http://rdf.rhea-db.org/Compound_5962; http://rdf.rhea-db.org/Compound_5962 rh:accession
1,4-dihydroxy-2-naphthoyl-CoA + H2O => 1,4-dihydroxy-2-naphthoate + CoA + H(+)
RHEA:26310		provides_input_for

Curated continuity through CHEBI:11173; no physical channeling or flux magnitude is asserted.

all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100
  • RHEA:26310

    Source assertion: RHEA:26310 explicitly has shared intermediate CHEBI:11173 in its products.

    Source locationhttp://rdf.rhea-db.org/26310 rh:products http://rdf.rhea-db.org/26309_R; http://rdf.rhea-db.org/26309_R rh:contains1 http://rdf.rhea-db.org/Participant_26309_compound_6107; http://rdf.rhea-db.org/Participant_26309_compound_6107 rh:compound http://rdf.rhea-db.org/Compound_6107; http://rdf.rhea-db.org/Compound_6107 rh:accession
  • RHEA:30100

    Source assertion: RHEA:30100 explicitly has shared intermediate CHEBI:11173 in its substrates.

    Source locationhttp://rdf.rhea-db.org/30100 rh:substrates http://rdf.rhea-db.org/30099_L; http://rdf.rhea-db.org/30099_L rh:contains1 http://rdf.rhea-db.org/Participant_30099_compound_6107; http://rdf.rhea-db.org/Participant_30099_compound_6107 rh:compound http://rdf.rhea-db.org/Compound_6107; http://rdf.rhea-db.org/Compound_6107 rh:accession
5 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate => all-trans-octaprenyl diphosphate + 5 diphosphate
RHEA:27799		provides_input_for

Curated continuity through CHEBI:57711; no physical channeling or flux magnitude is asserted.

all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100
  • RHEA:27799

    Source assertion: RHEA:27799 explicitly has shared intermediate CHEBI:57711 in its products.

    Source locationhttp://rdf.rhea-db.org/27799 rh:products http://rdf.rhea-db.org/27798_R; http://rdf.rhea-db.org/27798_R rh:contains1 http://rdf.rhea-db.org/Participant_27798_compound_1744; http://rdf.rhea-db.org/Participant_27798_compound_1744 rh:compound http://rdf.rhea-db.org/Compound_1744; http://rdf.rhea-db.org/Compound_1744 rh:accession
  • RHEA:30100

    Source assertion: RHEA:30100 explicitly has shared intermediate CHEBI:57711 in its substrates.

    Source locationhttp://rdf.rhea-db.org/30100 rh:substrates http://rdf.rhea-db.org/30099_L; http://rdf.rhea-db.org/30099_L rh:contains1 http://rdf.rhea-db.org/Participant_30099_compound_1744; http://rdf.rhea-db.org/Participant_30099_compound_1744 rh:compound http://rdf.rhea-db.org/Compound_1744; http://rdf.rhea-db.org/Compound_1744 rh:accession
all-trans-octaprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) => 2-demethylmenaquinol-8 + CO2 + diphosphate
RHEA:30100		provides_input_for

Curated continuity through CHEBI:61873; no physical channeling or flux magnitude is asserted.

2-demethylmenaquinol-8 + S-adenosyl-L-methionine => menaquinol-8 + S-adenosyl-L-homocysteine + H(+)
RHEA:30064
  • RHEA:30100

    Source assertion: RHEA:30100 explicitly has shared intermediate CHEBI:61873 in its products.

    Source locationhttp://rdf.rhea-db.org/30100 rh:products http://rdf.rhea-db.org/30099_R; http://rdf.rhea-db.org/30099_R rh:contains1 http://rdf.rhea-db.org/Participant_30099_compound_6609; http://rdf.rhea-db.org/Participant_30099_compound_6609 rh:compound http://rdf.rhea-db.org/Compound_6609; http://rdf.rhea-db.org/Compound_6609 rh:accession
  • RHEA:30064

    Source assertion: RHEA:30064 explicitly has shared intermediate CHEBI:61873 in its substrates.

    Source locationhttp://rdf.rhea-db.org/30064 rh:substrates http://rdf.rhea-db.org/30063_L; http://rdf.rhea-db.org/30063_L rh:contains1 http://rdf.rhea-db.org/Participant_30063_compound_6609; http://rdf.rhea-db.org/Participant_30063_compound_6609 rh:compound http://rdf.rhea-db.org/Compound_6609; http://rdf.rhea-db.org/Compound_6609 rh:accession

References

Curation history