Chorismate isomerization
MetaCyc:ISOCHORSYN-RXN | has_input Coefficient 1. | chorismate
MetaCyc:CHORISMATE | - MetaCyc:ISOCHORSYN-RXN
Source assertion: The native left slot specifies CHORISMATE with coefficient 1. The source direction is REVERSIBLE; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="ISOCHORSYN-RXN"]/left/*[@frameid="CHORISMATE"]; left/coefficient; reaction-direction - MetaCyc:CHORISMATE
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="CHORISMATE"]/common-name; cml/molecule; inchi
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Chorismate isomerization
MetaCyc:ISOCHORSYN-RXN | has_output Coefficient 1. | isochorismate
MetaCyc:ISOCHORISMATE | - MetaCyc:ISOCHORSYN-RXN
Source assertion: The native right slot specifies ISOCHORISMATE with coefficient 1. The source direction is REVERSIBLE; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="ISOCHORSYN-RXN"]/right/*[@frameid="ISOCHORISMATE"]; right/coefficient; reaction-direction - MetaCyc:ISOCHORISMATE
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="ISOCHORISMATE"]/common-name; cml/molecule; inchi
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Isochorismate hydrolysis
MetaCyc:ISOCHORMAT-RXN | has_input Coefficient 1. | H2O
MetaCyc:WATER | - MetaCyc:ISOCHORMAT-RXN
Source assertion: The native left slot specifies WATER with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="ISOCHORMAT-RXN"]/left/*[@frameid="WATER"]; left/coefficient; reaction-direction - MetaCyc:WATER
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="WATER"]/common-name; cml/molecule; inchi
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Isochorismate hydrolysis
MetaCyc:ISOCHORMAT-RXN | has_input Coefficient 1. | isochorismate
MetaCyc:ISOCHORISMATE | - MetaCyc:ISOCHORMAT-RXN
Source assertion: The native left slot specifies ISOCHORISMATE with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="ISOCHORMAT-RXN"]/left/*[@frameid="ISOCHORISMATE"]; left/coefficient; reaction-direction - MetaCyc:ISOCHORISMATE
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="ISOCHORISMATE"]/common-name; cml/molecule; inchi
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Isochorismate hydrolysis
MetaCyc:ISOCHORMAT-RXN | has_output Coefficient 1. | pyruvate
MetaCyc:PYRUVATE | - MetaCyc:ISOCHORMAT-RXN
Source assertion: The native right slot specifies PYRUVATE with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="ISOCHORMAT-RXN"]/right/*[@frameid="PYRUVATE"]; right/coefficient; reaction-direction - MetaCyc:PYRUVATE
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PYRUVATE"]/common-name; cml/molecule; inchi
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Isochorismate hydrolysis
MetaCyc:ISOCHORMAT-RXN | has_output Coefficient 1. | (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate
MetaCyc:DIHYDRO-DIOH-BENZOATE | - MetaCyc:ISOCHORMAT-RXN
Source assertion: The native right slot specifies DIHYDRO-DIOH-BENZOATE with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="ISOCHORMAT-RXN"]/right/*[@frameid="DIHYDRO-DIOH-BENZOATE"]; right/coefficient; reaction-direction - MetaCyc:DIHYDRO-DIOH-BENZOATE
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="DIHYDRO-DIOH-BENZOATE"]/common-name; cml/molecule; inchi
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Dihydrodihydroxybenzoate oxidation
MetaCyc:DHBDEHYD-RXN | has_input Coefficient 1. | (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate
MetaCyc:DIHYDRO-DIOH-BENZOATE | - MetaCyc:DHBDEHYD-RXN
Source assertion: The native left slot specifies DIHYDRO-DIOH-BENZOATE with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBDEHYD-RXN"]/left/*[@frameid="DIHYDRO-DIOH-BENZOATE"]; left/coefficient; reaction-direction - MetaCyc:DIHYDRO-DIOH-BENZOATE
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="DIHYDRO-DIOH-BENZOATE"]/common-name; cml/molecule; inchi
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Dihydrodihydroxybenzoate oxidation
MetaCyc:DHBDEHYD-RXN | has_input Coefficient 1. | NAD+
MetaCyc:NAD | - MetaCyc:DHBDEHYD-RXN
Source assertion: The native left slot specifies NAD with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBDEHYD-RXN"]/left/*[@frameid="NAD"]; left/coefficient; reaction-direction - MetaCyc:NAD
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="NAD"]/common-name; cml/molecule; inchi
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Dihydrodihydroxybenzoate oxidation
MetaCyc:DHBDEHYD-RXN | has_output Coefficient 1. | 2,3-dihydroxybenzoate
MetaCyc:2-3-DIHYDROXYBENZOATE | - MetaCyc:DHBDEHYD-RXN
Source assertion: The native right slot specifies 2-3-DIHYDROXYBENZOATE with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBDEHYD-RXN"]/right/*[@frameid="2-3-DIHYDROXYBENZOATE"]; right/coefficient; reaction-direction - MetaCyc:2-3-DIHYDROXYBENZOATE
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="2-3-DIHYDROXYBENZOATE"]/common-name; cml/molecule; inchi
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Dihydrodihydroxybenzoate oxidation
MetaCyc:DHBDEHYD-RXN | has_output Coefficient 1. | NADH(2-)
CHEBI:57945 | - MetaCyc:DHBDEHYD-RXN
Source assertion: The native right slot specifies NADH with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBDEHYD-RXN"]/right/*[@frameid="NADH"]; right/coefficient; reaction-direction - MetaCyc:NADH
Source assertion: The native CML and InChI define the ionic structure selected as CHEBI:57945; identity is checked independently against the complete ChEBI term, not its display name. Source locationCompound[@frameid="NADH"]/cml/molecule; inchi - CHEBI:30616
Source assertion: CHEBI:57945 has the same complete InChI, elemental formula and charge as this native compound; the selected label is its canonical ChEBI label. Source locationstatements WHERE subject="CHEBI:57945"; chemrof:inchi_string, chemrof:generalized_empirical_formula, chemrof:charge, rdfs:label
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Dihydrodihydroxybenzoate oxidation
MetaCyc:DHBDEHYD-RXN | has_output Coefficient 1. | H+
MetaCyc:PROTON | - MetaCyc:DHBDEHYD-RXN
Source assertion: The native right slot specifies PROTON with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBDEHYD-RXN"]/right/*[@frameid="PROTON"]; right/coefficient; reaction-direction - MetaCyc:PROTON
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PROTON"]/common-name; cml/molecule; inchi
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2,3-Dihydroxybenzoate adenylation
MetaCyc:DHBAMPLIG-RXN | has_input Coefficient 1. | H+
MetaCyc:PROTON | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The native left slot specifies PROTON with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBAMPLIG-RXN"]/left/*[@frameid="PROTON"]; left/coefficient; reaction-direction - MetaCyc:PROTON
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PROTON"]/common-name; cml/molecule; inchi
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2,3-Dihydroxybenzoate adenylation
MetaCyc:DHBAMPLIG-RXN | has_input Coefficient 1. | 2,3-dihydroxybenzoate
MetaCyc:2-3-DIHYDROXYBENZOATE | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The native left slot specifies 2-3-DIHYDROXYBENZOATE with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBAMPLIG-RXN"]/left/*[@frameid="2-3-DIHYDROXYBENZOATE"]; left/coefficient; reaction-direction - MetaCyc:2-3-DIHYDROXYBENZOATE
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="2-3-DIHYDROXYBENZOATE"]/common-name; cml/molecule; inchi
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2,3-Dihydroxybenzoate adenylation
MetaCyc:DHBAMPLIG-RXN | has_input Coefficient 1. | ATP(4-)
CHEBI:30616 | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The native left slot specifies ATP with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBAMPLIG-RXN"]/left/*[@frameid="ATP"]; left/coefficient; reaction-direction - MetaCyc:ATP
Source assertion: The native CML and InChI define the ionic structure selected as CHEBI:30616; identity is checked independently against the complete ChEBI term, not its display name. Source locationCompound[@frameid="ATP"]/cml/molecule; inchi - CHEBI:30616
Source assertion: CHEBI:30616 has the same complete InChI, elemental formula and charge as this native compound; the selected label is its canonical ChEBI label. Source locationstatements WHERE subject="CHEBI:30616"; chemrof:inchi_string, chemrof:generalized_empirical_formula, chemrof:charge, rdfs:label
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2,3-Dihydroxybenzoate adenylation
MetaCyc:DHBAMPLIG-RXN | has_output Coefficient 1. | (2,3-dihydroxybenzoyl)adenylate
MetaCyc:CPD-62 | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The native right slot specifies CPD-62 with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBAMPLIG-RXN"]/right/*[@frameid="CPD-62"]; right/coefficient; reaction-direction - MetaCyc:CPD-62
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="CPD-62"]/common-name; cml/molecule; inchi
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2,3-Dihydroxybenzoate adenylation
MetaCyc:DHBAMPLIG-RXN | has_output Coefficient 1. | diphosphate
MetaCyc:PPI | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The native right slot specifies PPI with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="DHBAMPLIG-RXN"]/right/*[@frameid="PPI"]; right/coefficient; reaction-direction - MetaCyc:PPI
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PPI"]/common-name; cml/molecule; inchi
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2,3-Dihydroxybenzoyl loading of DhbB
MetaCyc:RXN-19449 | has_input Coefficient 1. | (2,3-dihydroxybenzoyl)adenylate
MetaCyc:CPD-62 | - MetaCyc:RXN-19449
Source assertion: The native left slot specifies CPD-62 with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19449"]/left/*[@frameid="CPD-62"]; left/coefficient; reaction-direction - MetaCyc:CPD-62
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="CPD-62"]/common-name; cml/molecule; inchi
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2,3-Dihydroxybenzoyl loading of DhbB
MetaCyc:RXN-19449 | has_input Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a holo-[DhbB aryl-carrier protein]
MetaCyc:Holo-DhbB | - MetaCyc:RXN-19449
Source assertion: The native left slot specifies Holo-DhbB with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19449"]/left/*[@frameid="Holo-DhbB"]; left/coefficient; reaction-direction - MetaCyc:Holo-DhbB
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="Holo-DhbB"]/common-name; parent
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2,3-Dihydroxybenzoyl loading of DhbB
MetaCyc:RXN-19449 | has_output Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a 2,3-dihydroxybenzoyl-[DhbB aryl-carrier protein]
MetaCyc:23-dihydroxybenzoyl-DhbB | - MetaCyc:RXN-19449
Source assertion: The native right slot specifies 23-dihydroxybenzoyl-DhbB with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19449"]/right/*[@frameid="23-dihydroxybenzoyl-DhbB"]; right/coefficient; reaction-direction - MetaCyc:23-dihydroxybenzoyl-DhbB
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="23-dihydroxybenzoyl-DhbB"]/common-name; parent; unmodified-form
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2,3-Dihydroxybenzoyl loading of DhbB
MetaCyc:RXN-19449 | has_output Coefficient 1. | adenosine 5'-monophosphate(2-)
CHEBI:456215 | - MetaCyc:RXN-19449
Source assertion: The native right slot specifies AMP with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19449"]/right/*[@frameid="AMP"]; right/coefficient; reaction-direction - MetaCyc:AMP
Source assertion: The native CML and InChI define the ionic structure selected as CHEBI:456215; identity is checked independently against the complete ChEBI term, not its display name. Source locationCompound[@frameid="AMP"]/cml/molecule; inchi - CHEBI:30616
Source assertion: CHEBI:456215 has the same complete InChI, elemental formula and charge as this native compound; the selected label is its canonical ChEBI label. Source locationstatements WHERE subject="CHEBI:456215"; chemrof:inchi_string, chemrof:generalized_empirical_formula, chemrof:charge, rdfs:label
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2,3-Dihydroxybenzoyl loading of DhbB
MetaCyc:RXN-19449 | has_output Coefficient 1. | H+
MetaCyc:PROTON | - MetaCyc:RXN-19449
Source assertion: The native right slot specifies PROTON with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19449"]/right/*[@frameid="PROTON"]; right/coefficient; reaction-direction - MetaCyc:PROTON
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PROTON"]/common-name; cml/molecule; inchi
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Glycine adenylation
MetaCyc:RXN-9376 | has_input Coefficient 1. | glycine
MetaCyc:GLY | - MetaCyc:RXN-9376
Source assertion: The native left slot specifies GLY with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-9376"]/left/*[@frameid="GLY"]; left/coefficient; reaction-direction - MetaCyc:GLY
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="GLY"]/common-name; cml/molecule; inchi
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Glycine adenylation
MetaCyc:RXN-9376 | has_input Coefficient 1. | ATP(4-)
CHEBI:30616 | - MetaCyc:RXN-9376
Source assertion: The native left slot specifies ATP with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-9376"]/left/*[@frameid="ATP"]; left/coefficient; reaction-direction - MetaCyc:ATP
Source assertion: The native CML and InChI define the ionic structure selected as CHEBI:30616; identity is checked independently against the complete ChEBI term, not its display name. Source locationCompound[@frameid="ATP"]/cml/molecule; inchi - CHEBI:30616
Source assertion: CHEBI:30616 has the same complete InChI, elemental formula and charge as this native compound; the selected label is its canonical ChEBI label. Source locationstatements WHERE subject="CHEBI:30616"; chemrof:inchi_string, chemrof:generalized_empirical_formula, chemrof:charge, rdfs:label
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Glycine adenylation
MetaCyc:RXN-9376 | has_output Coefficient 1. | (glycyl)adenylate
MetaCyc:CPD-9993 | - MetaCyc:RXN-9376
Source assertion: The native right slot specifies CPD-9993 with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-9376"]/right/*[@frameid="CPD-9993"]; right/coefficient; reaction-direction - MetaCyc:CPD-9993
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="CPD-9993"]/common-name; cml/molecule; inchi
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Glycine adenylation
MetaCyc:RXN-9376 | has_output Coefficient 1. | diphosphate
MetaCyc:PPI | - MetaCyc:RXN-9376
Source assertion: The native right slot specifies PPI with coefficient 1. The source direction is LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-9376"]/right/*[@frameid="PPI"]; right/coefficient; reaction-direction - MetaCyc:PPI
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PPI"]/common-name; cml/molecule; inchi
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Glycyl loading of DhbF
MetaCyc:RXN-19458 | has_input Coefficient 1. | (glycyl)adenylate
MetaCyc:CPD-9993 | - MetaCyc:RXN-19458
Source assertion: The native left slot specifies CPD-9993 with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19458"]/left/*[@frameid="CPD-9993"]; left/coefficient; reaction-direction - MetaCyc:CPD-9993
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="CPD-9993"]/common-name; cml/molecule; inchi
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Glycyl loading of DhbF
MetaCyc:RXN-19458 | has_input Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a [DhbF non-ribosomal peptide synthase]
MetaCyc:DhbF | - MetaCyc:RXN-19458
Source assertion: The native left slot specifies DhbF with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19458"]/left/*[@frameid="DhbF"]; left/coefficient; reaction-direction - MetaCyc:DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="DhbF"]/common-name; parent
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Glycyl loading of DhbF
MetaCyc:RXN-19458 | has_output Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a glycyl-[DhbF non-ribosomal peptide synthase]
MetaCyc:Glycyl-DhbF | - MetaCyc:RXN-19458
Source assertion: The native right slot specifies Glycyl-DhbF with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19458"]/right/*[@frameid="Glycyl-DhbF"]; right/coefficient; reaction-direction - MetaCyc:Glycyl-DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="Glycyl-DhbF"]/common-name; parent; unmodified-form
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Glycyl loading of DhbF
MetaCyc:RXN-19458 | has_output Coefficient 1. | adenosine 5'-monophosphate(2-)
CHEBI:456215 | - MetaCyc:RXN-19458
Source assertion: The native right slot specifies AMP with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19458"]/right/*[@frameid="AMP"]; right/coefficient; reaction-direction - MetaCyc:AMP
Source assertion: The native CML and InChI define the ionic structure selected as CHEBI:456215; identity is checked independently against the complete ChEBI term, not its display name. Source locationCompound[@frameid="AMP"]/cml/molecule; inchi - CHEBI:30616
Source assertion: CHEBI:456215 has the same complete InChI, elemental formula and charge as this native compound; the selected label is its canonical ChEBI label. Source locationstatements WHERE subject="CHEBI:456215"; chemrof:inchi_string, chemrof:generalized_empirical_formula, chemrof:charge, rdfs:label
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Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | has_input Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a 2,3-dihydroxybenzoyl-[DhbB aryl-carrier protein]
MetaCyc:23-dihydroxybenzoyl-DhbB | - MetaCyc:RXN-19460
Source assertion: The native left slot specifies 23-dihydroxybenzoyl-DhbB with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19460"]/left/*[@frameid="23-dihydroxybenzoyl-DhbB"]; left/coefficient; reaction-direction - MetaCyc:23-dihydroxybenzoyl-DhbB
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="23-dihydroxybenzoyl-DhbB"]/common-name; parent; unmodified-form
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Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | has_input Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a glycyl-[DhbF non-ribosomal peptide synthase]
MetaCyc:Glycyl-DhbF | - MetaCyc:RXN-19460
Source assertion: The native left slot specifies Glycyl-DhbF with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19460"]/left/*[@frameid="Glycyl-DhbF"]; left/coefficient; reaction-direction - MetaCyc:Glycyl-DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="Glycyl-DhbF"]/common-name; parent; unmodified-form
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Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | has_output Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a 2,3-dihydroxybenzoyl-glycyl-[DhbF non-ribosomal peptide synthase]
MetaCyc:DHB-glycyl-DhbF | - MetaCyc:RXN-19460
Source assertion: The native right slot specifies DHB-glycyl-DhbF with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19460"]/right/*[@frameid="DHB-glycyl-DhbF"]; right/coefficient; reaction-direction - MetaCyc:DHB-glycyl-DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="DHB-glycyl-DhbF"]/common-name; parent; unmodified-form
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Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | has_output Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a holo-[DhbB aryl-carrier protein]
MetaCyc:Holo-DhbB | - MetaCyc:RXN-19460
Source assertion: The native right slot specifies Holo-DhbB with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19460"]/right/*[@frameid="Holo-DhbB"]; right/coefficient; reaction-direction - MetaCyc:Holo-DhbB
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="Holo-DhbB"]/common-name; parent
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Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | has_output Coefficient 1. | H+
MetaCyc:PROTON | - MetaCyc:RXN-19460
Source assertion: The native right slot specifies PROTON with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19460"]/right/*[@frameid="PROTON"]; right/coefficient; reaction-direction - MetaCyc:PROTON
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PROTON"]/common-name; cml/molecule; inchi
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L-Threonine adenylation
MetaCyc:RXN-9377 | has_input Coefficient 1. | L-threonine
MetaCyc:THR | - MetaCyc:RXN-9377
Source assertion: The native left slot specifies THR with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-9377"]/left/*[@frameid="THR"]; left/coefficient; reaction-direction - MetaCyc:THR
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="THR"]/common-name; cml/molecule; inchi
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L-Threonine adenylation
MetaCyc:RXN-9377 | has_input Coefficient 1. | ATP(4-)
CHEBI:30616 | - MetaCyc:RXN-9377
Source assertion: The native left slot specifies ATP with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-9377"]/left/*[@frameid="ATP"]; left/coefficient; reaction-direction - MetaCyc:ATP
Source assertion: The native CML and InChI define the ionic structure selected as CHEBI:30616; identity is checked independently against the complete ChEBI term, not its display name. Source locationCompound[@frameid="ATP"]/cml/molecule; inchi - CHEBI:30616
Source assertion: CHEBI:30616 has the same complete InChI, elemental formula and charge as this native compound; the selected label is its canonical ChEBI label. Source locationstatements WHERE subject="CHEBI:30616"; chemrof:inchi_string, chemrof:generalized_empirical_formula, chemrof:charge, rdfs:label
|
L-Threonine adenylation
MetaCyc:RXN-9377 | has_input Coefficient 1. | H+
MetaCyc:PROTON | - MetaCyc:RXN-9377
Source assertion: The native left slot specifies PROTON with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-9377"]/left/*[@frameid="PROTON"]; left/coefficient; reaction-direction - MetaCyc:PROTON
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PROTON"]/common-name; cml/molecule; inchi
|
L-Threonine adenylation
MetaCyc:RXN-9377 | has_output Coefficient 1. | (L-threonyl)adenylate
MetaCyc:CPD-9994 | - MetaCyc:RXN-9377
Source assertion: The native right slot specifies CPD-9994 with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-9377"]/right/*[@frameid="CPD-9994"]; right/coefficient; reaction-direction - MetaCyc:CPD-9994
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="CPD-9994"]/common-name; cml/molecule; inchi
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L-Threonine adenylation
MetaCyc:RXN-9377 | has_output Coefficient 1. | diphosphate
MetaCyc:PPI | - MetaCyc:RXN-9377
Source assertion: The native right slot specifies PPI with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-9377"]/right/*[@frameid="PPI"]; right/coefficient; reaction-direction - MetaCyc:PPI
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PPI"]/common-name; cml/molecule; inchi
|
L-Threonyl loading of DhbF
MetaCyc:RXN-19452 | has_input Coefficient 1. | (L-threonyl)adenylate
MetaCyc:CPD-9994 | - MetaCyc:RXN-19452
Source assertion: The native left slot specifies CPD-9994 with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19452"]/left/*[@frameid="CPD-9994"]; left/coefficient; reaction-direction - MetaCyc:CPD-9994
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="CPD-9994"]/common-name; cml/molecule; inchi
|
L-Threonyl loading of DhbF
MetaCyc:RXN-19452 | has_input Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a [DhbF non-ribosomal peptide synthase]
MetaCyc:DhbF | - MetaCyc:RXN-19452
Source assertion: The native left slot specifies DhbF with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19452"]/left/*[@frameid="DhbF"]; left/coefficient; reaction-direction - MetaCyc:DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="DhbF"]/common-name; parent
|
L-Threonyl loading of DhbF
MetaCyc:RXN-19452 | has_output Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | an L-threonyl-[DhbF non-ribosomal peptide synthase]
MetaCyc:Threonyl-DhbF | - MetaCyc:RXN-19452
Source assertion: The native right slot specifies Threonyl-DhbF with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19452"]/right/*[@frameid="Threonyl-DhbF"]; right/coefficient; reaction-direction - MetaCyc:Threonyl-DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="Threonyl-DhbF"]/common-name; parent; unmodified-form
|
L-Threonyl loading of DhbF
MetaCyc:RXN-19452 | has_output Coefficient 1. | adenosine 5'-monophosphate(2-)
CHEBI:456215 | - MetaCyc:RXN-19452
Source assertion: The native right slot specifies AMP with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19452"]/right/*[@frameid="AMP"]; right/coefficient; reaction-direction - MetaCyc:AMP
Source assertion: The native CML and InChI define the ionic structure selected as CHEBI:456215; identity is checked independently against the complete ChEBI term, not its display name. Source locationCompound[@frameid="AMP"]/cml/molecule; inchi - CHEBI:30616
Source assertion: CHEBI:456215 has the same complete InChI, elemental formula and charge as this native compound; the selected label is its canonical ChEBI label. Source locationstatements WHERE subject="CHEBI:456215"; chemrof:inchi_string, chemrof:generalized_empirical_formula, chemrof:charge, rdfs:label
|
L-Threonyl loading of DhbF
MetaCyc:RXN-19452 | has_output Coefficient 1. | H+
MetaCyc:PROTON | - MetaCyc:RXN-19452
Source assertion: The native right slot specifies PROTON with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19452"]/right/*[@frameid="PROTON"]; right/coefficient; reaction-direction - MetaCyc:PROTON
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PROTON"]/common-name; cml/molecule; inchi
|
Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | has_input Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a 2,3-dihydroxybenzoyl-glycyl-[DhbF non-ribosomal peptide synthase]
MetaCyc:DHB-glycyl-DhbF | - MetaCyc:RXN-19461
Source assertion: The native left slot specifies DHB-glycyl-DhbF with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19461"]/left/*[@frameid="DHB-glycyl-DhbF"]; left/coefficient; reaction-direction - MetaCyc:DHB-glycyl-DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="DHB-glycyl-DhbF"]/common-name; parent; unmodified-form
|
Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | has_input Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | an L-threonyl-[DhbF non-ribosomal peptide synthase]
MetaCyc:Threonyl-DhbF | - MetaCyc:RXN-19461
Source assertion: The native left slot specifies Threonyl-DhbF with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19461"]/left/*[@frameid="Threonyl-DhbF"]; left/coefficient; reaction-direction - MetaCyc:Threonyl-DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="Threonyl-DhbF"]/common-name; parent; unmodified-form
|
Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | has_output Coefficient 1. Primary-supported threonyl reactive-state correction under the native transformation identity. The native arginyl frame and imported MNX sameAs are not accepted as exact equivalents. Coefficients count formal carrier states, not whole DhbF polypeptides. | a 2,3-dihydroxybenzoyl-glycyl-L-threonyl-[DhbF non-ribosomal peptide synthase]
metanetx.chemical:MNXM743865 | - MetaCyc:RXN-19461
Source assertion: The original right slot has coefficient 1 for DHB-glycyl-arginyl-DhbF. That arginyl name conflicts with the threonyl precursor and primary structure; this edge is a curated threonyl correction, not native-equation equivalence. Source locationReaction[@frameid="RXN-19461"]/right/Protein; right/coefficient - metanetx.chemical:MNXM743865
Source assertion: The independently issued canonical name is the DHB-glycyl-L-threonyl DhbF state; native SMILES encodes a 2S,3R threonyl thioester and protein-continuation wildcards. It is a reactive class, not an exact full protein or a sameAs claim to the old arginyl frame. Source locationUnique MolecularEntity JSON-LD: identifier, name, smiles; visible Properties: global charge -1 - PMID:39870587
Source assertion: Figure 1b depicts DHB-Gly-L-Thr bound to the second DhbF CP and its trimeric cyclorelease. Figure 5 supports L-Thr selection; Figure 6 detects reconstituted bacillibactin. Source locationPDF p.4 Figure 1b; p.8 Figure 5; pp.8-9 Figure 6
|
Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | has_output Coefficient 1. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a [DhbF non-ribosomal peptide synthase]
MetaCyc:DhbF | - MetaCyc:RXN-19461
Source assertion: The native right slot specifies DhbF with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19461"]/right/*[@frameid="DhbF"]; right/coefficient; reaction-direction - MetaCyc:DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="DhbF"]/common-name; parent
|
Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | has_output Coefficient 1. | H+
MetaCyc:PROTON | - MetaCyc:RXN-19461
Source assertion: The native right slot specifies PROTON with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19461"]/right/*[@frameid="PROTON"]; right/coefficient; reaction-direction - MetaCyc:PROTON
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="PROTON"]/common-name; cml/molecule; inchi
|
Bacillibactin trimerization and cyclorelease
MetaCyc:RXN-19462 | has_input Coefficient 3. Primary-supported threonyl reactive-state correction under the native transformation identity. The native arginyl frame and imported MNX sameAs are not accepted as exact equivalents. Coefficients count formal carrier states, not whole DhbF polypeptides. | a 2,3-dihydroxybenzoyl-glycyl-L-threonyl-[DhbF non-ribosomal peptide synthase]
metanetx.chemical:MNXM743865 | - MetaCyc:RXN-19462
Source assertion: The original left slot has coefficient 3 for DHB-glycyl-arginyl-DhbF. That arginyl name conflicts with the threonyl precursor and primary structure; this edge is a curated threonyl correction, not native-equation equivalence. Source locationReaction[@frameid="RXN-19462"]/left/Protein; left/coefficient - metanetx.chemical:MNXM743865
Source assertion: The independently issued canonical name is the DHB-glycyl-L-threonyl DhbF state; native SMILES encodes a 2S,3R threonyl thioester and protein-continuation wildcards. It is a reactive class, not an exact full protein or a sameAs claim to the old arginyl frame. Source locationUnique MolecularEntity JSON-LD: identifier, name, smiles; visible Properties: global charge -1 - PMID:39870587
Source assertion: Figure 1b depicts DHB-Gly-L-Thr bound to the second DhbF CP and its trimeric cyclorelease. Figure 5 supports L-Thr selection; Figure 6 detects reconstituted bacillibactin. Source locationPDF p.4 Figure 1b; p.8 Figure 5; pp.8-9 Figure 6
|
Bacillibactin trimerization and cyclorelease
MetaCyc:RXN-19462 | has_output Coefficient 1. | bacillibactin
MetaCyc:CPD-9984 | - MetaCyc:RXN-19462
Source assertion: The native right slot specifies CPD-9984 with coefficient 1. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19462"]/right/*[@frameid="CPD-9984"]; right/coefficient; reaction-direction - MetaCyc:CPD-9984
Source assertion: The native compound frame supplies its exact chemical structure, stereochemical InChI and charge; no neutral or unspecified-anomer cross-reference is substituted. Source locationCompound[@frameid="CPD-9984"]/common-name; cml/molecule; inchi
|
Bacillibactin trimerization and cyclorelease
MetaCyc:RXN-19462 | has_output Coefficient 3. Native carrier-state class, not an exact full polypeptide. DhbF contains two CP sites; state coefficients do not count whole NRPS molecules. | a [DhbF non-ribosomal peptide synthase]
MetaCyc:DhbF | - MetaCyc:RXN-19462
Source assertion: The native right slot specifies DhbF with coefficient 3. The source direction is PHYSIOL-LEFT-TO-RIGHT; the displayed side follows the biosynthetic transformation. Source locationReaction[@frameid="RXN-19462"]/right/*[@frameid="DhbF"]; right/coefficient; reaction-direction - MetaCyc:DhbF
Source assertion: This native protein class names the specified covalent carrier state. It does not identify a KH3-4 sequence or establish whole-enzyme stoichiometry. Source locationProtein[@frameid="DhbF"]/common-name; parent
|
isochorismate synthase DhbC [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45612.1 | enables Annotation-supported KH3-4 gene-product assignment for the complete upstream DHB module. No purified activity or active oligomer is established for this selected protein by the 2025 study. | Chorismate isomerization
MetaCyc:ISOCHORSYN-RXN | - NCBIProtein:BDG45612.1
Source assertion: The versioned GenPept entry BDG45612.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45612.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: The KH3-4 cluster on AP025627 contains dhbACB assigned to 2,3-DHB production from chorismate. These upstream activities were not individually assayed in the NRPS reconstitution that begins with supplied 2,3-DHB. Source locationPDF p.4, Results and Discussion, KH3-4 gene cluster; Figure 1a - NCBIProtein:BDG45612.1
Source assertion: The product annotation and similarity-based CDS inference support this proposed upstream activity. They are annotation evidence, not direct purified-enzyme evidence for the selected KH3-4 protein. Source locationGBSeq[GBSeq_accession-version="BDG45612.1"]/GBSeq_feature-table/GBFeature/GBFeature_quals (product, inference)
|
isochorismatase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45614.1 | enables Annotation-supported KH3-4 gene-product assignment for the complete upstream DHB module. No purified activity or active oligomer is established for this selected protein by the 2025 study. | Isochorismate hydrolysis
MetaCyc:ISOCHORMAT-RXN | - NCBIProtein:BDG45614.1
Source assertion: The versioned GenPept entry BDG45614.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45614.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: The KH3-4 cluster on AP025627 contains dhbACB assigned to 2,3-DHB production from chorismate. These upstream activities were not individually assayed in the NRPS reconstitution that begins with supplied 2,3-DHB. Source locationPDF p.4, Results and Discussion, KH3-4 gene cluster; Figure 1a - NCBIProtein:BDG45614.1
Source assertion: The product annotation and similarity-based CDS inference support this proposed upstream activity. They are annotation evidence, not direct purified-enzyme evidence for the selected KH3-4 protein. Source locationGBSeq[GBSeq_accession-version="BDG45614.1"]/GBSeq_feature-table/GBFeature/GBFeature_quals (product, inference)
|
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45611.1 | enables Annotation-supported KH3-4 gene-product assignment for the complete upstream DHB module. No purified activity or active oligomer is established for this selected protein by the 2025 study. | Dihydrodihydroxybenzoate oxidation
MetaCyc:DHBDEHYD-RXN | - NCBIProtein:BDG45611.1
Source assertion: The versioned GenPept entry BDG45611.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45611.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: The KH3-4 cluster on AP025627 contains dhbACB assigned to 2,3-DHB production from chorismate. These upstream activities were not individually assayed in the NRPS reconstitution that begins with supplied 2,3-DHB. Source locationPDF p.4, Results and Discussion, KH3-4 gene cluster; Figure 1a - NCBIProtein:BDG45611.1
Source assertion: The product annotation and similarity-based CDS inference support this proposed upstream activity. They are annotation evidence, not direct purified-enzyme evidence for the selected KH3-4 protein. Source locationGBSeq[GBSeq_accession-version="BDG45611.1"]/GBSeq_feature-table/GBFeature/GBFeature_quals (product, inference)
|
2,3-dihydroxybenzoate-AMP ligase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45613.1 | enables Functional gene-product role supported by the primary mechanistic model and KH3-4-derived recombinant preparations. This does not establish monomer sufficiency, fixed assembly stoichiometry or separately measured flux for every carrier-bound step. | 2,3-Dihydroxybenzoate adenylation
MetaCyc:DHBAMPLIG-RXN | - NCBIProtein:BDG45613.1
Source assertion: The versioned GenPept entry BDG45613.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45613.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: DhbE preferentially activates 2,3-DHB; the loading model transfers the activated acyl group to DhbB. The purified KH3-4-derived preparations support complete NRPS product formation, not a separate kinetic assay of every bound transfer. Source locationPDF p.4 Figure 1b; pp.7-8 Figure 5 and substrate-specificity paragraphs; pp.8-9 Figure 6 - PMID:39870587
Source assertion: Methods explicitly identify BDG45613 as the KH3-4 gene source for the recombinant protein. Tags and coexpression mean the preparation is not claimed to be an independently sequence-verified native full protein. Source locationPDF p.12, Experimental Section, Plasmid construction; pp.12-13 Production and purification
|
2,3-dihydroxybenzoate-AMP ligase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45613.1 | enables Functional gene-product role supported by the primary mechanistic model and KH3-4-derived recombinant preparations. This does not establish monomer sufficiency, fixed assembly stoichiometry or separately measured flux for every carrier-bound step. | 2,3-Dihydroxybenzoyl loading of DhbB
MetaCyc:RXN-19449 | - NCBIProtein:BDG45613.1
Source assertion: The versioned GenPept entry BDG45613.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45613.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: DhbE preferentially activates 2,3-DHB; the loading model transfers the activated acyl group to DhbB. The purified KH3-4-derived preparations support complete NRPS product formation, not a separate kinetic assay of every bound transfer. Source locationPDF p.4 Figure 1b; pp.7-8 Figure 5 and substrate-specificity paragraphs; pp.8-9 Figure 6 - PMID:39870587
Source assertion: Methods explicitly identify BDG45613 as the KH3-4 gene source for the recombinant protein. Tags and coexpression mean the preparation is not claimed to be an independently sequence-verified native full protein. Source locationPDF p.12, Experimental Section, Plasmid construction; pp.12-13 Production and purification
|
dimodular nonribosomal peptide synthase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45615.1 | enables Functional gene-product role supported by the primary mechanistic model and KH3-4-derived recombinant preparations. This does not establish monomer sufficiency, fixed assembly stoichiometry or separately measured flux for every carrier-bound step. | Glycine adenylation
MetaCyc:RXN-9376 | - NCBIProtein:BDG45615.1
Source assertion: The versioned GenPept entry BDG45615.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45615.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: DhbF module 1 prefers glycine and module 2 L-threonine. Figure 1b assigns loading, condensations and TE cyclorelease; purified KH3-4-derived NRPS preparations generate bacillibactin. Not every bound transfer was assayed separately. Source locationPDF p.4 Figure 1b; pp.7-8 Figure 5 and substrate-specificity paragraphs; pp.8-9 Figure 6 - PMID:39870587
Source assertion: Methods explicitly identify BDG45615 as the KH3-4 gene source for the recombinant protein. Tags and coexpression mean the preparation is not claimed to be an independently sequence-verified native full protein. Source locationPDF p.12, Experimental Section, Plasmid construction; pp.12-13 Production and purification
|
dimodular nonribosomal peptide synthase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45615.1 | enables Functional gene-product role supported by the primary mechanistic model and KH3-4-derived recombinant preparations. This does not establish monomer sufficiency, fixed assembly stoichiometry or separately measured flux for every carrier-bound step. | Glycyl loading of DhbF
MetaCyc:RXN-19458 | - NCBIProtein:BDG45615.1
Source assertion: The versioned GenPept entry BDG45615.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45615.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: DhbF module 1 prefers glycine and module 2 L-threonine. Figure 1b assigns loading, condensations and TE cyclorelease; purified KH3-4-derived NRPS preparations generate bacillibactin. Not every bound transfer was assayed separately. Source locationPDF p.4 Figure 1b; pp.7-8 Figure 5 and substrate-specificity paragraphs; pp.8-9 Figure 6 - PMID:39870587
Source assertion: Methods explicitly identify BDG45615 as the KH3-4 gene source for the recombinant protein. Tags and coexpression mean the preparation is not claimed to be an independently sequence-verified native full protein. Source locationPDF p.12, Experimental Section, Plasmid construction; pp.12-13 Production and purification
|
dimodular nonribosomal peptide synthase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45615.1 | enables Functional gene-product role supported by the primary mechanistic model and KH3-4-derived recombinant preparations. This does not establish monomer sufficiency, fixed assembly stoichiometry or separately measured flux for every carrier-bound step. | Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | - NCBIProtein:BDG45615.1
Source assertion: The versioned GenPept entry BDG45615.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45615.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: DhbF module 1 prefers glycine and module 2 L-threonine. Figure 1b assigns loading, condensations and TE cyclorelease; purified KH3-4-derived NRPS preparations generate bacillibactin. Not every bound transfer was assayed separately. Source locationPDF p.4 Figure 1b; pp.7-8 Figure 5 and substrate-specificity paragraphs; pp.8-9 Figure 6 - PMID:39870587
Source assertion: Methods explicitly identify BDG45615 as the KH3-4 gene source for the recombinant protein. Tags and coexpression mean the preparation is not claimed to be an independently sequence-verified native full protein. Source locationPDF p.12, Experimental Section, Plasmid construction; pp.12-13 Production and purification
|
dimodular nonribosomal peptide synthase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45615.1 | enables Functional gene-product role supported by the primary mechanistic model and KH3-4-derived recombinant preparations. This does not establish monomer sufficiency, fixed assembly stoichiometry or separately measured flux for every carrier-bound step. | L-Threonine adenylation
MetaCyc:RXN-9377 | - NCBIProtein:BDG45615.1
Source assertion: The versioned GenPept entry BDG45615.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45615.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: DhbF module 1 prefers glycine and module 2 L-threonine. Figure 1b assigns loading, condensations and TE cyclorelease; purified KH3-4-derived NRPS preparations generate bacillibactin. Not every bound transfer was assayed separately. Source locationPDF p.4 Figure 1b; pp.7-8 Figure 5 and substrate-specificity paragraphs; pp.8-9 Figure 6 - PMID:39870587
Source assertion: Methods explicitly identify BDG45615 as the KH3-4 gene source for the recombinant protein. Tags and coexpression mean the preparation is not claimed to be an independently sequence-verified native full protein. Source locationPDF p.12, Experimental Section, Plasmid construction; pp.12-13 Production and purification
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dimodular nonribosomal peptide synthase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45615.1 | enables Functional gene-product role supported by the primary mechanistic model and KH3-4-derived recombinant preparations. This does not establish monomer sufficiency, fixed assembly stoichiometry or separately measured flux for every carrier-bound step. | L-Threonyl loading of DhbF
MetaCyc:RXN-19452 | - NCBIProtein:BDG45615.1
Source assertion: The versioned GenPept entry BDG45615.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45615.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: DhbF module 1 prefers glycine and module 2 L-threonine. Figure 1b assigns loading, condensations and TE cyclorelease; purified KH3-4-derived NRPS preparations generate bacillibactin. Not every bound transfer was assayed separately. Source locationPDF p.4 Figure 1b; pp.7-8 Figure 5 and substrate-specificity paragraphs; pp.8-9 Figure 6 - PMID:39870587
Source assertion: Methods explicitly identify BDG45615 as the KH3-4 gene source for the recombinant protein. Tags and coexpression mean the preparation is not claimed to be an independently sequence-verified native full protein. Source locationPDF p.12, Experimental Section, Plasmid construction; pp.12-13 Production and purification
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dimodular nonribosomal peptide synthase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45615.1 | enables Functional gene-product role supported by the primary mechanistic model and KH3-4-derived recombinant preparations. This does not establish monomer sufficiency, fixed assembly stoichiometry or separately measured flux for every carrier-bound step. | Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | - NCBIProtein:BDG45615.1
Source assertion: The versioned GenPept entry BDG45615.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45615.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: DhbF module 1 prefers glycine and module 2 L-threonine. Figure 1b assigns loading, condensations and TE cyclorelease; purified KH3-4-derived NRPS preparations generate bacillibactin. Not every bound transfer was assayed separately. Source locationPDF p.4 Figure 1b; pp.7-8 Figure 5 and substrate-specificity paragraphs; pp.8-9 Figure 6 - PMID:39870587
Source assertion: Methods explicitly identify BDG45615 as the KH3-4 gene source for the recombinant protein. Tags and coexpression mean the preparation is not claimed to be an independently sequence-verified native full protein. Source locationPDF p.12, Experimental Section, Plasmid construction; pp.12-13 Production and purification
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dimodular nonribosomal peptide synthase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45615.1 | enables Functional gene-product role supported by the primary mechanistic model and KH3-4-derived recombinant preparations. This does not establish monomer sufficiency, fixed assembly stoichiometry or separately measured flux for every carrier-bound step. | Bacillibactin trimerization and cyclorelease
MetaCyc:RXN-19462 | - NCBIProtein:BDG45615.1
Source assertion: The versioned GenPept entry BDG45615.1 identifies Parageobacillus sp. KH3-4, taxon 2916802, and its AP025627.1 coding region. The native gene product is distinguished from tagged experimental constructs and carrier classes. Source locationGBSeq[GBSeq_accession-version="BDG45615.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals (coded_by, db_xref, locus_tag) - PMID:39870587
Source assertion: DhbF module 1 prefers glycine and module 2 L-threonine. Figure 1b assigns loading, condensations and TE cyclorelease; purified KH3-4-derived NRPS preparations generate bacillibactin. Not every bound transfer was assayed separately. Source locationPDF p.4 Figure 1b; pp.7-8 Figure 5 and substrate-specificity paragraphs; pp.8-9 Figure 6 - PMID:39870587
Source assertion: Methods explicitly identify BDG45615 as the KH3-4 gene source for the recombinant protein. Tags and coexpression mean the preparation is not claimed to be an independently sequence-verified native full protein. Source locationPDF p.12, Experimental Section, Plasmid construction; pp.12-13 Production and purification
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Chorismate isomerization
MetaCyc:ISOCHORSYN-RXN | part_of | MetaCyc:PWY-5903 | - MetaCyc:PWY-5901
Source assertion: The original reaction-list includes ISOCHORSYN-RXN; this complete module member is retained in the chorismate-to-bacillibactin route. Source locationPathway[@frameid="PWY-5901"]/reaction-list/Reaction - MetaCyc:PWY-5903
Source assertion: PWY-5903 explicitly includes PWY-5901 as its upstream nested pathway. Source locationPathway[@frameid="PWY-5903"]/reaction-list/Pathway
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Isochorismate hydrolysis
MetaCyc:ISOCHORMAT-RXN | part_of | MetaCyc:PWY-5903 | - MetaCyc:PWY-5901
Source assertion: The original reaction-list includes ISOCHORMAT-RXN; this complete module member is retained in the chorismate-to-bacillibactin route. Source locationPathway[@frameid="PWY-5901"]/reaction-list/Reaction - MetaCyc:PWY-5903
Source assertion: PWY-5903 explicitly includes PWY-5901 as its upstream nested pathway. Source locationPathway[@frameid="PWY-5903"]/reaction-list/Pathway
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Dihydrodihydroxybenzoate oxidation
MetaCyc:DHBDEHYD-RXN | part_of | MetaCyc:PWY-5903 | - MetaCyc:PWY-5901
Source assertion: The original reaction-list includes DHBDEHYD-RXN; this complete module member is retained in the chorismate-to-bacillibactin route. Source locationPathway[@frameid="PWY-5901"]/reaction-list/Reaction - MetaCyc:PWY-5903
Source assertion: PWY-5903 explicitly includes PWY-5901 as its upstream nested pathway. Source locationPathway[@frameid="PWY-5903"]/reaction-list/Pathway
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2,3-Dihydroxybenzoate adenylation
MetaCyc:DHBAMPLIG-RXN | part_of | MetaCyc:PWY-5903 | |
2,3-Dihydroxybenzoyl loading of DhbB
MetaCyc:RXN-19449 | part_of | MetaCyc:PWY-5903 | |
Glycine adenylation
MetaCyc:RXN-9376 | part_of | MetaCyc:PWY-5903 | |
Glycyl loading of DhbF
MetaCyc:RXN-19458 | part_of | MetaCyc:PWY-5903 | |
Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | part_of | MetaCyc:PWY-5903 | |
L-Threonine adenylation
MetaCyc:RXN-9377 | part_of | MetaCyc:PWY-5903 | |
L-Threonyl loading of DhbF
MetaCyc:RXN-19452 | part_of | MetaCyc:PWY-5903 | |
Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | part_of | MetaCyc:PWY-5903 | |
Bacillibactin trimerization and cyclorelease
MetaCyc:RXN-19462 | part_of | MetaCyc:PWY-5903 | |
Chorismate isomerization
MetaCyc:ISOCHORSYN-RXN | provides_input_for Shared intermediate: MetaCyc:ISOCHORISMATE. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | Isochorismate hydrolysis
MetaCyc:ISOCHORMAT-RXN | |
Isochorismate hydrolysis
MetaCyc:ISOCHORMAT-RXN | provides_input_for Shared intermediate: MetaCyc:DIHYDRO-DIOH-BENZOATE. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | Dihydrodihydroxybenzoate oxidation
MetaCyc:DHBDEHYD-RXN | |
Dihydrodihydroxybenzoate oxidation
MetaCyc:DHBDEHYD-RXN | provides_input_for Shared intermediate: MetaCyc:2-3-DIHYDROXYBENZOATE. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | 2,3-Dihydroxybenzoate adenylation
MetaCyc:DHBAMPLIG-RXN | |
2,3-Dihydroxybenzoate adenylation
MetaCyc:DHBAMPLIG-RXN | provides_input_for Shared intermediate: MetaCyc:CPD-62. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | 2,3-Dihydroxybenzoyl loading of DhbB
MetaCyc:RXN-19449 | |
2,3-Dihydroxybenzoyl loading of DhbB
MetaCyc:RXN-19449 | provides_input_for Shared intermediate: MetaCyc:23-dihydroxybenzoyl-DhbB. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | |
Glycine adenylation
MetaCyc:RXN-9376 | provides_input_for Shared intermediate: MetaCyc:CPD-9993. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | Glycyl loading of DhbF
MetaCyc:RXN-19458 | |
Glycyl loading of DhbF
MetaCyc:RXN-19458 | provides_input_for Shared intermediate: MetaCyc:Glycyl-DhbF. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | |
Dihydroxybenzoyl-glycyl condensation
MetaCyc:RXN-19460 | provides_input_for Shared intermediate: MetaCyc:DHB-glycyl-DhbF. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | |
L-Threonine adenylation
MetaCyc:RXN-9377 | provides_input_for Shared intermediate: MetaCyc:CPD-9994. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | L-Threonyl loading of DhbF
MetaCyc:RXN-19452 | |
L-Threonyl loading of DhbF
MetaCyc:RXN-19452 | provides_input_for Shared intermediate: MetaCyc:Threonyl-DhbF. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | |
Dihydroxybenzoyl-glycyl-L-threonyl condensation
MetaCyc:RXN-19461 | provides_input_for Shared intermediate: metanetx.chemical:MNXM743865. This is a chemical handoff, not an assertion of physical channeling or a single linear activation order. | Bacillibactin trimerization and cyclorelease
MetaCyc:RXN-19462 | - PMID:39870587
Source assertion: The second DhbF condensation domain forms the DHB-Gly-L-Thr carrier-bound unit; the TE domain then mediates trimeric cyclorelease to bacillibactin. Source locationPDF p.4 Figure 1b and caption - metanetx.chemical:MNXM743865
Source assertion: The selected intermediate is the independently named DHB-glycyl-L-threonyl DhbF reactive class; old arginyl cross-references are not exact mappings. Source locationUnique MolecularEntity JSON-LD: identifier, name, smiles
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4'-phosphopantetheinyl transferase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45617.1 | activates Activation by phosphopantetheinylation in the KH3-4-derived preparation; DhbB modification was measured directly. The printed coexpression primer mismatch does not prove that an incorrect plasmid was used. | isochorismatase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45614.1 | - NCBIProtein:BDG45617.1
Source assertion: The original versioned KH3-4 GenPept entry identifies the cluster 4-prime-phosphopantetheinyl transferase and its AP025627.1 coding region. Source locationGBSeq[GBSeq_accession-version="BDG45617.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals - PMID:39870587
Source assertion: Methods identify BDG45617 as the KH3-4 PPTase gene and BDG45614/BDG45615 as DhbB/DhbF. The proteins were expressed with tags and/or coexpression; this identifies native gene sources, not fully verified engineered construct sequences. Source locationPDF p.12, Plasmid construction; pp.12-13, Production and purification - PMID:39870587
Source assertion: Purified PPTase and CoA increase apo-DhbB mass by about 340 Da. The no-PPTase control and coexpression preparation support phosphopantetheinylation. The printed coexpression primer discrepancy limits exact construct reconstruction. Source locationPDF pp.5-6, PPTase Results and Figure 3; p.14, PPTase assay; p.19, SI Table S1
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4'-phosphopantetheinyl transferase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45617.1 | activates PPTase-dependent activation model supported by coexpression and function. Both DhbF carrier-site occupancies were not directly measured; this is weaker evidence than the measured DhbB mass shift. | dimodular nonribosomal peptide synthase [Parageobacillus sp. KH3-4]
NCBIProtein:BDG45615.1 | - NCBIProtein:BDG45617.1
Source assertion: The original versioned KH3-4 GenPept entry identifies the cluster 4-prime-phosphopantetheinyl transferase and its AP025627.1 coding region. Source locationGBSeq[GBSeq_accession-version="BDG45617.1"]/GBSeq_definition; GBSeq_feature-table/GBFeature/GBFeature_quals - PMID:39870587
Source assertion: Methods identify BDG45617 as the KH3-4 PPTase gene and BDG45614/BDG45615 as DhbB/DhbF. The proteins were expressed with tags and/or coexpression; this identifies native gene sources, not fully verified engineered construct sequences. Source locationPDF p.12, Plasmid construction; pp.12-13, Production and purification - PMID:39870587
Source assertion: DhbF was coexpressed with PPTase and the resulting preparation was used as holo-DhbF in active NRPS reconstitution. Its large mass excluded direct MALDI modification analysis; full site occupancy was not independently measured. Source locationPDF pp.5-7, PPTase Results and Figure 3 discussion; pp.12-14, coexpression and reconstitution Methods
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