5-aminoimidazole ribonucleotide biosynthesis II

MetaCyc:PWY-6122

5-Aminoimidazole ribonucleotide biosynthesis II converts 5-phospho-alpha-D-ribose 1-diphosphate to 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole through 5-phospho-beta-D-ribosylamine, glycinamide ribonucleotide, ATP- and formate-dependent formylation to FGAR, and ATP-dependent amidine formation and cyclization.

Pathway type

nucleotide biosynthesis

Organism scope

Escherichia coli NCBITaxon:562

Read the source YAML

Pathway network

34 nodes · 54 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Other participant
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

Show at full size (scroll to explore)

Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Escherichia coli `NCBITaxon:562`	taxon
amidophosphoribosyltransferase `EC:2.4.2.14`	molecular activity
phosphoribosylamine-glycine ligase `EC:6.3.4.13`	molecular activity
phosphoribosylglycinamide formyltransferase 2 `EC:6.3.1.21`	molecular activity
phosphoribosylformylglycinamidine synthase `EC:6.3.5.3`	molecular activity
phosphoribosylformylglycinamidine cyclo-ligase `EC:6.3.3.1`	molecular activity
5-phospho-alpha-D-ribose 1-diphosphate `CHEBI:58017`	small molecule
L-glutamine `CHEBI:58359`	small molecule
H2O `CHEBI:15377`	small molecule
5-phospho-beta-D-ribosylamine `CHEBI:58681`	small molecule
L-glutamate `CHEBI:29985`	small molecule
diphosphate `CHEBI:33019`	small molecule
glycine `CHEBI:57305`	small molecule
ATP `CHEBI:30616`	small molecule
N(1)-(5-phospho-beta-D-ribosyl)glycinamide `CHEBI:143788`	small molecule
ADP `CHEBI:456216`	small molecule
phosphate `CHEBI:43474`	small molecule
H(+) `CHEBI:15378`	small molecule
formate `CHEBI:15740`	small molecule
N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide `CHEBI:147286`	small molecule
2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine `CHEBI:147287`	small molecule
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole `CHEBI:137981`	small molecule
Phosphoribosylformylglycinamidine cyclo-ligase `UniProtKB:P08178`	protein
cytoplasm `GO:0005737`	cellular component
Amidophosphoribosyltransferase `UniProtKB:P0AG16`	protein
magnesium(2+) `CHEBI:18420`	cofactor
Phosphoribosylformylglycinamidine synthase `UniProtKB:P15254`	protein
Phosphoribosylamine--glycine ligase `UniProtKB:P15640`	protein
manganese(2+) `CHEBI:29035`	cofactor
Formate-dependent phosphoribosylglycinamide formyltransferase `UniProtKB:P33221`	protein
amidophosphoribosyltransferase reaction `RHEA:14907`	reaction	right to left
phosphoribosylamine-glycine ligase reaction `RHEA:17454`	reaction	left to right
phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	reaction	left to right
phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	reaction	left to right
phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	reaction	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
H2O `CHEBI:15377`	consumes	amidophosphoribosyltransferase reaction `RHEA:14907`	RHEA:14907 Source assertion: Rhea lists CHEBI:15377 on the substrates side of RHEA:14907 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14907']/rh:substrates; rh:contains/rh:compound/rh:accession`
5-phospho-alpha-D-ribose 1-diphosphate `CHEBI:58017`	consumes	amidophosphoribosyltransferase reaction `RHEA:14907`	RHEA:14907 Source assertion: Rhea lists CHEBI:58017 on the substrates side of RHEA:14907 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14907']/rh:substrates; rh:contains/rh:compound/rh:accession`
L-glutamine `CHEBI:58359`	consumes	amidophosphoribosyltransferase reaction `RHEA:14907`	RHEA:14907 Source assertion: Rhea lists CHEBI:58359 on the substrates side of RHEA:14907 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14907']/rh:substrates; rh:contains/rh:compound/rh:accession`
amidophosphoribosyltransferase reaction `RHEA:14907`	produces	L-glutamate `CHEBI:29985`	RHEA:14907 Source assertion: Rhea lists CHEBI:29985 on the products side of RHEA:14907 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14907']/rh:products; rh:contains/rh:compound/rh:accession`
amidophosphoribosyltransferase reaction `RHEA:14907`	produces	diphosphate `CHEBI:33019`	RHEA:14907 Source assertion: Rhea lists CHEBI:33019 on the products side of RHEA:14907 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14907']/rh:products; rh:contains/rh:compound/rh:accession`
amidophosphoribosyltransferase reaction `RHEA:14907`	produces	5-phospho-beta-D-ribosylamine `CHEBI:58681`	RHEA:14907 Source assertion: Rhea lists CHEBI:58681 on the products side of RHEA:14907 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/14907']/rh:products; rh:contains/rh:compound/rh:accession`
ATP `CHEBI:30616`	consumes	phosphoribosylamine-glycine ligase reaction `RHEA:17454`	RHEA:17454 Source assertion: Rhea lists CHEBI:30616 on the substrates side of RHEA:17454 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17454']/rh:substrates; rh:contains/rh:compound/rh:accession`
glycine `CHEBI:57305`	consumes	phosphoribosylamine-glycine ligase reaction `RHEA:17454`	RHEA:17454 Source assertion: Rhea lists CHEBI:57305 on the substrates side of RHEA:17454 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17454']/rh:substrates; rh:contains/rh:compound/rh:accession`
5-phospho-beta-D-ribosylamine `CHEBI:58681`	consumes	phosphoribosylamine-glycine ligase reaction `RHEA:17454`	RHEA:17454 Source assertion: Rhea lists CHEBI:58681 on the substrates side of RHEA:17454 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17454']/rh:substrates; rh:contains/rh:compound/rh:accession`
phosphoribosylamine-glycine ligase reaction `RHEA:17454`	produces	N(1)-(5-phospho-beta-D-ribosyl)glycinamide `CHEBI:143788`	RHEA:17454 Source assertion: Rhea lists CHEBI:143788 on the products side of RHEA:17454 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17454']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylamine-glycine ligase reaction `RHEA:17454`	produces	H(+) `CHEBI:15378`	RHEA:17454 Source assertion: Rhea lists CHEBI:15378 on the products side of RHEA:17454 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17454']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylamine-glycine ligase reaction `RHEA:17454`	produces	phosphate `CHEBI:43474`	RHEA:17454 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:17454 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17454']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylamine-glycine ligase reaction `RHEA:17454`	produces	ADP `CHEBI:456216`	RHEA:17454 Source assertion: Rhea lists CHEBI:456216 on the products side of RHEA:17454 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17454']/rh:products; rh:contains/rh:compound/rh:accession`
N(1)-(5-phospho-beta-D-ribosyl)glycinamide `CHEBI:143788`	consumes	phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	RHEA:24830 Source assertion: Rhea lists CHEBI:143788 on the substrates side of RHEA:24830 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24830']/rh:substrates; rh:contains/rh:compound/rh:accession`
formate `CHEBI:15740`	consumes	phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	RHEA:24830 Source assertion: Rhea lists CHEBI:15740 on the substrates side of RHEA:24830 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24830']/rh:substrates; rh:contains/rh:compound/rh:accession`
ATP `CHEBI:30616`	consumes	phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	RHEA:24830 Source assertion: Rhea lists CHEBI:30616 on the substrates side of RHEA:24830 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24830']/rh:substrates; rh:contains/rh:compound/rh:accession`
phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	produces	N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide `CHEBI:147286`	RHEA:24830 Source assertion: Rhea lists CHEBI:147286 on the products side of RHEA:24830 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24830']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	produces	H(+) `CHEBI:15378`	RHEA:24830 Source assertion: Rhea lists CHEBI:15378 on the products side of RHEA:24830 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24830']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	produces	phosphate `CHEBI:43474`	RHEA:24830 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:24830 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24830']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	produces	ADP `CHEBI:456216`	RHEA:24830 Source assertion: Rhea lists CHEBI:456216 on the products side of RHEA:24830 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/24830']/rh:products; rh:contains/rh:compound/rh:accession`
N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide `CHEBI:147286`	consumes	phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	RHEA:17130 Source assertion: Rhea lists CHEBI:147286 on the substrates side of RHEA:17130 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17130']/rh:substrates; rh:contains/rh:compound/rh:accession`
H2O `CHEBI:15377`	consumes	phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	RHEA:17130 Source assertion: Rhea lists CHEBI:15377 on the substrates side of RHEA:17130 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17130']/rh:substrates; rh:contains/rh:compound/rh:accession`
ATP `CHEBI:30616`	consumes	phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	RHEA:17130 Source assertion: Rhea lists CHEBI:30616 on the substrates side of RHEA:17130 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17130']/rh:substrates; rh:contains/rh:compound/rh:accession`
L-glutamine `CHEBI:58359`	consumes	phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	RHEA:17130 Source assertion: Rhea lists CHEBI:58359 on the substrates side of RHEA:17130 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17130']/rh:substrates; rh:contains/rh:compound/rh:accession`
phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	produces	2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine `CHEBI:147287`	RHEA:17130 Source assertion: Rhea lists CHEBI:147287 on the products side of RHEA:17130 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17130']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	produces	H(+) `CHEBI:15378`	RHEA:17130 Source assertion: Rhea lists CHEBI:15378 on the products side of RHEA:17130 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17130']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	produces	L-glutamate `CHEBI:29985`	RHEA:17130 Source assertion: Rhea lists CHEBI:29985 on the products side of RHEA:17130 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17130']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	produces	phosphate `CHEBI:43474`	RHEA:17130 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:17130 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17130']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	produces	ADP `CHEBI:456216`	RHEA:17130 Source assertion: Rhea lists CHEBI:456216 on the products side of RHEA:17130 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/17130']/rh:products; rh:contains/rh:compound/rh:accession`
2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine `CHEBI:147287`	consumes	phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	RHEA:23033 Source assertion: Rhea lists CHEBI:147287 on the substrates side of RHEA:23033 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/23033']/rh:substrates; rh:contains/rh:compound/rh:accession`
ATP `CHEBI:30616`	consumes	phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	RHEA:23033 Source assertion: Rhea lists CHEBI:30616 on the substrates side of RHEA:23033 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/23033']/rh:substrates; rh:contains/rh:compound/rh:accession`
phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	produces	5-amino-1-(5-phospho-beta-D-ribosyl)imidazole `CHEBI:137981`	RHEA:23033 Source assertion: Rhea lists CHEBI:137981 on the products side of RHEA:23033 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/23033']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	produces	H(+) `CHEBI:15378`	RHEA:23033 Source assertion: Rhea lists CHEBI:15378 on the products side of RHEA:23033 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/23033']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	produces	phosphate `CHEBI:43474`	RHEA:23033 Source assertion: Rhea lists CHEBI:43474 on the products side of RHEA:23033 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/23033']/rh:products; rh:contains/rh:compound/rh:accession`
phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	produces	ADP `CHEBI:456216`	RHEA:23033 Source assertion: Rhea lists CHEBI:456216 on the products side of RHEA:23033 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/23033']/rh:products; rh:contains/rh:compound/rh:accession`
amidophosphoribosyltransferase `EC:2.4.2.14`	catalyzes	amidophosphoribosyltransferase reaction `RHEA:14907`	RHEA:14905 Source assertion: Rhea associates EC:2.4.2.14 with the reaction family containing RHEA:14907. Source location `rh:ec`
phosphoribosylamine-glycine ligase `EC:6.3.4.13`	catalyzes	phosphoribosylamine-glycine ligase reaction `RHEA:17454`	RHEA:17453 Source assertion: Rhea associates EC:6.3.4.13 with the reaction family containing RHEA:17454. Source location `rh:ec`
phosphoribosylglycinamide formyltransferase 2 `EC:6.3.1.21`	catalyzes	phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	RHEA:24829 Source assertion: Rhea associates EC:6.3.1.21 with the reaction family containing RHEA:24830. Source location `rh:ec`
phosphoribosylformylglycinamidine synthase `EC:6.3.5.3`	catalyzes	phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	RHEA:17129 Source assertion: Rhea associates EC:6.3.5.3 with the reaction family containing RHEA:17130. Source location `rh:ec`
phosphoribosylformylglycinamidine cyclo-ligase `EC:6.3.3.1`	catalyzes	phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	RHEA:23032 Source assertion: Rhea associates EC:6.3.3.1 with the reaction family containing RHEA:23033. Source location `rh:ec`
amidophosphoribosyltransferase reaction `RHEA:14907`	precedes	phosphoribosylamine-glycine ligase reaction `RHEA:17454`	MetaCyc:PWY-6122 Source assertion: MetaCyc pathway ordering places RHEA:14907 before RHEA:17454 in this pathway. Shared intermediate: CHEBI:58681. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	precedes	phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	MetaCyc:PWY-6122 Source assertion: MetaCyc pathway ordering places RHEA:17130 before RHEA:23033 in this pathway. Shared intermediate: CHEBI:147287. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
phosphoribosylamine-glycine ligase reaction `RHEA:17454`	precedes	phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	MetaCyc:PWY-6122 Source assertion: MetaCyc pathway ordering places RHEA:17454 before RHEA:24830 in this pathway. Shared intermediate: CHEBI:143788. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	precedes	phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	MetaCyc:PWY-6122 Source assertion: MetaCyc pathway ordering places RHEA:24830 before RHEA:17130 in this pathway. Shared intermediate: CHEBI:147286. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
Phosphoribosylformylglycinamidine cyclo-ligase `UniProtKB:P08178`	enables	phosphoribosylformylglycinamidine cyclo-ligase reaction `RHEA:23033`	UniProtKB:P08178 Source assertion: UniProt annotates UniProtKB:P08178 with catalytic activity for the reaction family of RHEA:23033. Annotation evidence: ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Phosphoribosylformylglycinamidine cyclo-ligase `UniProtKB:P08178`	located_in	cytoplasm `GO:0005737`	UniProtKB:P08178 Source assertion: UniProt locates UniProtKB:P08178 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: curated source annotation. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Amidophosphoribosyltransferase `UniProtKB:P0AG16`	enables	amidophosphoribosyltransferase reaction `RHEA:14907`	UniProtKB:P0AG16 Source assertion: UniProt annotates UniProtKB:P0AG16 with catalytic activity for the reaction family of RHEA:14907. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Amidophosphoribosyltransferase `UniProtKB:P0AG16`	has_cofactor	magnesium(2+) `CHEBI:18420`	UniProtKB:P0AG16 Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:P0AG16 (evidence: ECO:0000255). Binds 1 Mg(2+) ion per subunit. Source location `comments[commentType='COFACTOR'].cofactors and note`
Phosphoribosylformylglycinamidine synthase `UniProtKB:P15254`	enables	phosphoribosylformylglycinamidine synthase reaction `RHEA:17130`	UniProtKB:P15254 Source assertion: UniProt annotates UniProtKB:P15254 with catalytic activity for the reaction family of RHEA:17130. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Phosphoribosylformylglycinamidine synthase `UniProtKB:P15254`	located_in	cytoplasm `GO:0005737`	UniProtKB:P15254 Source assertion: UniProt locates UniProtKB:P15254 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000255, ECO:0000269. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Phosphoribosylamine--glycine ligase `UniProtKB:P15640`	enables	phosphoribosylamine-glycine ligase reaction `RHEA:17454`	UniProtKB:P15640 Source assertion: UniProt annotates UniProtKB:P15640 with catalytic activity for the reaction family of RHEA:17454. Annotation evidence: ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Phosphoribosylamine--glycine ligase `UniProtKB:P15640`	has_cofactor	magnesium(2+) `CHEBI:18420`	UniProtKB:P15640 Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:P15640 (evidence: ECO:0000269). Binds 1 Mg(2+) or Mn(2+) ion per subunit. Source location `comments[commentType='COFACTOR'].cofactors and note`
Phosphoribosylamine--glycine ligase `UniProtKB:P15640`	has_cofactor	manganese(2+) `CHEBI:29035`	UniProtKB:P15640 Source assertion: UniProt lists CHEBI:29035 as a cofactor of UniProtKB:P15640 (evidence: ECO:0000250). Binds 1 Mg(2+) or Mn(2+) ion per subunit. Source location `comments[commentType='COFACTOR'].cofactors and note`
Formate-dependent phosphoribosylglycinamide formyltransferase `UniProtKB:P33221`	enables	phosphoribosylglycinamide formyltransferase 2 reaction `RHEA:24830`	UniProtKB:P33221 Source assertion: UniProt annotates UniProtKB:P33221 with catalytic activity for the reaction family of RHEA:24830. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:83333. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`

References

MetaCyc:PWY-6122 MetaCyc 5-aminoimidazole ribonucleotide biosynthesis II pathway (source version: 22.5)
RHEA:14905 Rhea amidophosphoribosyltransferase reaction (source version: 139)
RHEA:14907 Rhea 5-phospho-beta-D-ribosylamine-forming direction (source version: 139)
RHEA:17453 Rhea phosphoribosylamine-glycine ligase reaction (source version: 139)
RHEA:17454 Rhea glycinamide ribonucleotide-forming direction (source version: 139)
RHEA:24829 Rhea phosphoribosylglycinamide formyltransferase 2 reaction (source version: 139)
RHEA:24830 Rhea FGAR-forming direction (source version: 139)
RHEA:17129 Rhea phosphoribosylformylglycinamidine synthase reaction (source version: 139)
RHEA:17130 Rhea FGAM-forming direction (source version: 139)
RHEA:23032 Rhea phosphoribosylformylglycinamidine cyclo-ligase reaction (source version: 139)
RHEA:23033 Rhea AIR-forming direction (source version: 139)
UniProtKB:P08178 UniProtKB:P08178 authority record (source version: 2026_03)
UniProtKB:P0AG16 UniProtKB:P0AG16 authority record (source version: 2026_03)
UniProtKB:P15254 UniProtKB:P15254 authority record (source version: 2026_03)
UniProtKB:P15640 UniProtKB:P15640 authority record (source version: 2026_03)
UniProtKB:P33221 UniProtKB:P33221 authority record (source version: 2026_03)

Curation history

2026-10-05T06:25:52Z codex
- EDIT — Review causal chemistry and add sourced molecular context
  Reviewed the entire record against MetaCyc 22.5 native pathway XML (including nested pathways), Rhea 139 explicit sides and directions, ENZYME 02-Sep-2026, PubMed records, and reviewed UniProt 2026_03 annotations within the record's taxon scope. Replaced generated structured-source quotations with source_assertion and source_locator. Typed molecular activities and participants. Reaction count 5 to 5; 0 directed-ID corrections; 5 protein annotations, 3 cofactor annotations and 2 sourced locations. All Rhea reaction substrate/product sets checked against independent Rhea authority; native pathway leaf coverage and source exceptions recorded in reports/causal_graph_review/metacyc-review.json. Full source URLs, versions and byte hashes are in metacyc-sources.json. All 50 MetaCyc records passed native schema validation; six targeted chemistry/evidence regression tests passed. Missing protein or cofactor annotations are not treated as biological absence; qualifications and exclusions are preserved in the ledger.
Read this curation session