petrobactin biosynthesis

MetaCyc:PWY-6289

Complete ten-transformation native pathway including 3-dehydroshikimate conversion, carrier maturation and both convergent citrate/spermidine assembly branches. The organism context is Bacillus anthracis Sterne; the principal full pathway study used Sterne 34F2-derived preparations. The six versioned source proteins preserve native identities; current reviewed AsbB and AsbE annotations differ at their N termini, and exact tagged constructs are not equated to native sequences. Source-defined AsbF is dimeric; AsbB dimer evidence is retained on its functional role without assigning an unverified complex identity. The physiological catalyst of generic apo-to-holo carrier maturation remains unassigned. Generic carrier states represent whole chemical classes rather than full protein sequences. The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only. Both branches are supported by the primary in vitro study, while a unique intracellular order remains unresolved. AsbA partial substitution by AsbB and early free-aroylspermidine side products do not expand the selected native boundary. The inspected early-enzyme paper is abstract-only and the later study supplement was unavailable. The selected citryl intermediate identities leave absolute citrate-derived stereochemistry unspecified; they do not assert racemic physiology or acceptance of every stereoisomer.

Pathway type
petrobactin biosynthesis
Organism scope

Read the source YAML

Pathway network

40 nodes · 94 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Pathway network: petrobactin biosynthesis 40 nodes · 94 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. RHEA-COMP:17557 — consumes → RHEA:48405; This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:48405consumes CHEBI:57287 — consumes → RHEA:48405; Evidence: RHEA:48405consumes RHEA:48405 — produces → RHEA-COMP:15903; This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:48405produces RHEA:48405 — produces → CHEBI:58343; Evidence: RHEA:48405produces RHEA:48405 — produces → CHEBI:15378; Evidence: RHEA:48405produces CHEBI:16630 — consumes → RHEA:24849; Evidence: RHEA:24849consumes RHEA:24849 — produces → CHEBI:36241; Evidence: RHEA:24849produces RHEA:24849 — produces → CHEBI:15377; Evidence: RHEA:24849produces CHEBI:36241 — consumes → RHEA:62469; Evidence: RHEA:62469consumes CHEBI:30616 — consumes → RHEA:62469; Evidence: RHEA:62469consumes CHEBI:15378 — consumes → RHEA:62469; Evidence: RHEA:62469consumes RHEA:62469 — produces → CHEBI:144942; Evidence: RHEA:62469produces RHEA:62469 — produces → CHEBI:33019; Evidence: RHEA:62469produces CHEBI:144942 — consumes → RHEA:62477; Evidence: RHEA:62477consumes RHEA-COMP:15903 — consumes → RHEA:62477; This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:62477consumes RHEA:62477 — produces → RHEA-COMP:15941; This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:62477produces RHEA:62477 — produces → CHEBI:456215; Evidence: RHEA:62477produces RHEA:62477 — produces → CHEBI:15378; Evidence: RHEA:62477produces CHEBI:57834 — consumes → RHEA:63809; Evidence: RHEA:63809consumes CHEBI:16947 — consumes → RHEA:63809; Evidence: RHEA:63809consumes CHEBI:30616 — consumes → RHEA:63809; Evidence: RHEA:63809consumes RHEA:63809 — produces → CHEBI:149586; The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.; Evidence: RHEA:63809produces RHEA:63809 — produces → CHEBI:456215; Evidence: RHEA:63809produces RHEA:63809 — produces → CHEBI:33019; Evidence: RHEA:63809produces RHEA:63809 — produces → CHEBI:15378; Evidence: RHEA:63809produces CHEBI:149586 — consumes → RHEA:63813; The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.; Evidence: RHEA:63813consumes CHEBI:57834 — consumes → RHEA:63813; Evidence: RHEA:63813consumes CHEBI:30616 — consumes → RHEA:63813; Evidence: RHEA:63813consumes RHEA:63813 — produces → CHEBI:149592; Evidence: RHEA:63813produces RHEA:63813 — produces → CHEBI:456215; Evidence: RHEA:63813produces RHEA:63813 — produces → CHEBI:33019; Evidence: RHEA:63813produces RHEA:63813 — produces → CHEBI:15378; Evidence: RHEA:63813produces CHEBI:149586 — consumes → RHEA:64029; The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.; Evidence: RHEA:64029consumes RHEA-COMP:15941 — consumes → RHEA:64029; This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:64029consumes RHEA:64029 — produces → CHEBI:149593; The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.; Evidence: RHEA:64029produces RHEA:64029 — produces → RHEA-COMP:15903; This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:64029produces RHEA:64029 — produces → CHEBI:15378; Evidence: RHEA:64029produces CHEBI:149593 — consumes → RHEA:63817; The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.; Evidence: RHEA:63817consumes CHEBI:57834 — consumes → RHEA:63817; Evidence: RHEA:63817consumes CHEBI:30616 — consumes → RHEA:63817; Evidence: RHEA:63817consumes RHEA:63817 — produces → CHEBI:149594; The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.; Evidence: RHEA:63817produces RHEA:63817 — produces → CHEBI:456215; Evidence: RHEA:63817produces RHEA:63817 — produces → CHEBI:33019; Evidence: RHEA:63817produces RHEA:63817 — produces → CHEBI:15378; Evidence: RHEA:63817produces CHEBI:149592 — consumes → RHEA:64025; Evidence: RHEA:64025consumes RHEA-COMP:15941 — consumes → RHEA:64025; This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:64025consumes RHEA:64025 — produces → CHEBI:149594; The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.; Evidence: RHEA:64025produces RHEA:64025 — produces → RHEA-COMP:15903; This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:64025produces RHEA:64025 — produces → CHEBI:15378; Evidence: RHEA:64025produces CHEBI:149594 — consumes → MetaCyc:RXN-10679; The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only. The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.; Evidence: MetaCyc:RXN-10679, MetaCyc:CPD-11481, CHEBI:149594consumes RHEA-COMP:15941 — consumes → MetaCyc:RXN-10679; The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: MetaCyc:RXN-10679, RHEA:63821consumes MetaCyc:RXN-10679 — produces → MetaCyc:CPD-9985; The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only.; Evidence: MetaCyc:RXN-10679, MetaCyc:CPD-9985, PMID:22408253, CHEBI:142778produces MetaCyc:RXN-10679 — produces → RHEA-COMP:15903; The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: MetaCyc:RXN-10679, RHEA:63821, MetaCyc:Aryl-Carrier-Proteins, PMID:22408253produces MetaCyc:RXN-10679 — produces → CHEBI:15378; The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only.; Evidence: MetaCyc:RXN-10679produces MetaCyc:CPLX-7874 — catalyzes → RHEA:24849; The native species-level complex is applied to the experimentally sourced Sterne AsbF dimer. Exact tagged constructs are not equated to the unmodified gene product.; Evidence: MetaCyc:CPLX-7874, PMID:18955706catalyzes NCBIProtein:AAT54155.1 — enables → RHEA:62469; AsbC gene-product role; the original Sterne translation equals the current reviewed sequence. The two native component transformations are retained without additionally counting the overlapping Rhea net reaction. Exact tagged constructs and a fixed oligomeric state are not established here.; Evidence: NCBIProtein:AAT54155.1, UniProtKB:Q81RQ7, PMID:17346033enables NCBIProtein:AAT54155.1 — enables → RHEA:62477; AsbC gene-product role; the original Sterne translation equals the current reviewed sequence. The two native component transformations are retained without additionally counting the overlapping Rhea net reaction. Exact tagged constructs and a fixed oligomeric state are not established here.; Evidence: NCBIProtein:AAT54155.1, UniProtKB:Q81RQ7, PMID:17346033enables NCBIProtein:AAT54153.1 — enables → RHEA:63809; AsbA gene-product activity does not assert monomer sufficiency. The original Sterne translation equals the current reviewed sequence. Magnesium-dependent assay context is retained without an independently established metal-binding cofactor relation. AsbB can partly substitute in vitro, so this edge does not assert universal AsbA indispensability.; Evidence: NCBIProtein:AAT54153.1, UniProtKB:Q81RQ9, PMID:22408253enables NCBIProtein:AAT54154.1 — enables → RHEA:63813; The versioned Sterne AsbB translation is 610 residues; current reviewed Q81RQ8 has two additional N-terminal residues. The primary supports a dimeric preparation,; its gene-product enables relation does not establish monomer sufficiency. Exact recombinant construct equality is not established.; Evidence: NCBIProtein:AAT54154.1, UniProtKB:Q81RQ8, PMID:22408253enables NCBIProtein:AAT54157.1 — enables → RHEA:64029; The versioned Sterne AsbE translation is 331 residues; current reviewed Q81RQ5 matches its suffix after four residues. The role is grounded in the Sterne source and primary functional assignment, not full-sequence equality to the current annotation. Its fixed assembly is unresolved.; Evidence: NCBIProtein:AAT54157.1, UniProtKB:Q81RQ5, PMID:22408253enables NCBIProtein:AAT54154.1 — enables → RHEA:63817; The versioned Sterne AsbB translation is 610 residues; current reviewed Q81RQ8 has two additional N-terminal residues. The primary supports a dimeric preparation,; its gene-product enables relation does not establish monomer sufficiency. Exact recombinant construct equality is not established.; Evidence: NCBIProtein:AAT54154.1, UniProtKB:Q81RQ8, PMID:22408253enables NCBIProtein:AAT54157.1 — enables → RHEA:64025; The versioned Sterne AsbE translation is 331 residues; current reviewed Q81RQ5 matches its suffix after four residues. The role is grounded in the Sterne source and primary functional assignment, not full-sequence equality to the current annotation. Its fixed assembly is unresolved. The bis(spermidine) branch uses the current database single-transfer step; the primary observed final product and proposed successive transfers because the monoaryl intermediate did not accumulate.; Evidence: NCBIProtein:AAT54157.1, UniProtKB:Q81RQ5, PMID:22408253enables NCBIProtein:AAT54157.1 — enables → MetaCyc:RXN-10679; The versioned Sterne AsbE translation is 331 residues; current reviewed Q81RQ5 matches its suffix after four residues. The role is grounded in the Sterne source and primary functional assignment, not full-sequence equality to the current annotation. Its fixed assembly is unresolved. The authentic native terminal product is retained despite the current ChEBI connectivity conflict.; Evidence: NCBIProtein:AAT54157.1, UniProtKB:Q81RQ5, PMID:22408253enables RHEA:48405 — part_of → MetaCyc:PWY-6289; The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.; Evidence: MetaCyc:PWY-6289, RHEA:48405part of RHEA:24849 — part_of → MetaCyc:PWY-6289; The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.; Evidence: MetaCyc:PWY-6289, RHEA:24849part of RHEA:62469 — part_of → MetaCyc:PWY-6289; The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.; Evidence: MetaCyc:PWY-6289, RHEA:62469part of RHEA:62477 — part_of → MetaCyc:PWY-6289; The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.; Evidence: MetaCyc:PWY-6289, RHEA:62477part of RHEA:63809 — part_of → MetaCyc:PWY-6289; The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.; Evidence: MetaCyc:PWY-6289, RHEA:63809part of RHEA:63813 — part_of → MetaCyc:PWY-6289; The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.; Evidence: MetaCyc:PWY-6289, RHEA:63813part of RHEA:64029 — part_of → MetaCyc:PWY-6289; The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.; Evidence: MetaCyc:PWY-6289, RHEA:64029part of RHEA:63817 — part_of → MetaCyc:PWY-6289; The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.; Evidence: MetaCyc:PWY-6289, RHEA:63817part of RHEA:64025 — part_of → MetaCyc:PWY-6289; The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.; Evidence: MetaCyc:PWY-6289, RHEA:64025part of MetaCyc:RXN-10679 — part_of → MetaCyc:PWY-6289; The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only.; Evidence: MetaCyc:PWY-6289, MetaCyc:RXN-10679part of NCBIProtein:AAT54153.1 — part_of → NCBITaxon:260799; Evidence: NCBIProtein:AAT54153.1part of NCBIProtein:AAT54154.1 — part_of → NCBITaxon:260799; Evidence: NCBIProtein:AAT54154.1part of NCBIProtein:AAT54155.1 — part_of → NCBITaxon:260799; Evidence: NCBIProtein:AAT54155.1part of NCBIProtein:AAT54156.1 — part_of → NCBITaxon:260799; Evidence: NCBIProtein:AAT54156.1part of NCBIProtein:AAT54157.1 — part_of → NCBITaxon:260799; Evidence: NCBIProtein:AAT54157.1part of NCBIProtein:AAT54158.1 — part_of → NCBITaxon:260799; Evidence: NCBIProtein:AAT54158.1part of MetaCyc:CPLX-7874 — has_part → NCBIProtein:AAT54158.1; Two AsbF subunits per source-defined dimer. The source Sterne translation and current reviewed Q81RQ4 sequence compare exactly; generic carrier-state counts elsewhere are not subunit counts.; Evidence: MetaCyc:CPLX-7874, MetaCyc:MONOMER-14940, NCBIProtein:AAT54158.1, UniProtKB:Q81RQ4, PMID:18955706has part MetaCyc:CPLX-7874 — has_cofactor → CHEBI:29035; Manganese is the experimentally preferred bound metal in the inspected preparation; the edge does not assert exclusive physiological metal use.; Evidence: UniProtKB:Q81RQ4, PMID:18955706has cofactor NCBIProtein:AAT54156.1 — part_of → MetaCyc:PWY-6289; The selected versioned Sterne AsbD protein participates in petrobactin biosynthesis. This contextual participation does not assert an exact identity between AsbD and every member of the generic carrier classes.; Evidence: NCBIProtein:AAT54156.1, UniProtKB:Q81RQ6, PMID:17346033part of RHEA:48405 — provides_input_for → RHEA:62477; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:48405, RHEA:62477provides input for RHEA:24849 — provides_input_for → RHEA:62469; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.; Evidence: RHEA:24849, RHEA:62469provides input for RHEA:62469 — provides_input_for → RHEA:62477; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.; Evidence: RHEA:62469, RHEA:62477provides input for RHEA:63809 — provides_input_for → RHEA:63813; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.; Evidence: RHEA:63809, RHEA:63813provides input for RHEA:63809 — provides_input_for → RHEA:64029; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.; Evidence: RHEA:63809, RHEA:64029provides input for RHEA:64029 — provides_input_for → RHEA:63817; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.; Evidence: RHEA:64029, RHEA:63817provides input for RHEA:63813 — provides_input_for → RHEA:64025; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.; Evidence: RHEA:63813, RHEA:64025provides input for RHEA:63817 — provides_input_for → MetaCyc:RXN-10679; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.; Evidence: RHEA:63817, MetaCyc:RXN-10679provides input for RHEA:64025 — provides_input_for → MetaCyc:RXN-10679; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.; Evidence: RHEA:64025, MetaCyc:RXN-10679provides input for RHEA:62477 — provides_input_for → RHEA:64029; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:62477, RHEA:64029provides input for RHEA:62477 — provides_input_for → RHEA:64025; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:62477, RHEA:64025provides input for RHEA:62477 — provides_input_for → MetaCyc:RXN-10679; The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.; Evidence: RHEA:62477, MetaCyc:RXN-10679provides input for apo-[aryl-carrier protein]; RHEA-COMP:17557; Declared type: chemical_entity chemical entity apo-[aryl-carrier protein] coenzyme A(4-); CHEBI:57287; Declared type: small_molecule small molecule coenzyme A(4-) holo-[aryl-carrier protein]; RHEA-COMP:15903; Declared type: chemical_entity chemical entity holo-[aryl-carrier protein] adenosine 3',5'-bismonophosphate(4-); CHEBI:58343; Declared type: small_molecule small molecule adenosine 3',5'-bismonophosphate(4-) hydron; CHEBI:15378; Declared type: small_molecule small molecule hydron 3-dehydroshikimate; CHEBI:16630; Declared type: small_molecule small molecule 3-dehydroshikimate 3,4-dihydroxybenzoate; CHEBI:36241; Declared type: small_molecule small molecule 3,4-dihydroxybenzoate water; CHEBI:15377; Declared type: small_molecule small molecule water ATP(4-); CHEBI:30616; Declared type: small_molecule small molecule ATP(4-) 3,4-dihydroxybenzoyl-AMP(1-); CHEBI:144942; Declared type: small_molecule small molecule 3,4-dihydroxybenzoyl-AMP(1-) diphosphate(3-); CHEBI:33019; Declared type: small_molecule small molecule diphosphate(3-) 3,4-dihydroxybenzoyl-[aryl-carrier protein]; RHEA-COMP:15941; Declared type: chemical_entity chemical entity 3,4-dihydroxybenzoyl-[aryl- carrier protein] adenosine 5'-monophosphate(2-); CHEBI:456215; Declared type: small_molecule small molecule adenosine 5'-monophosphate(2-) spermidine(3+); CHEBI:57834; Declared type: small_molecule small molecule spermidine(3+) citrate(3-); CHEBI:16947; Declared type: small_molecule small molecule citrate(3-) N-citryl-spermidine zwitterion; CHEBI:149586; Declared type: small_molecule small molecule N-citryl-spermidine zwitterion N(8),N'(8)-citryl-bis(spermidine) (+3); CHEBI:149592; Declared type: small_molecule small molecule N(8),N'(8)-citryl- bis(spermidine) (+3) N(1)-(3,4-dihydroxybenzoyl)-N(8)-citryl-spermidine (1-); CHEBI:149593; Declared type: small_molecule small molecule N(1)-(3,4-dihydroxybenzoyl)- N(8)-citryl-spermidine (1-) N(1)-(3,4-dihydroxybenzoyl)-N(8),N'(8)-citryl-bis(spermidine) (2+); CHEBI:149594; Declared type: small_molecule small molecule N(1)-(3,4-dihydroxybenzoyl)- N(8),N'(8)-citryl- bis(spermidine) (2+) petrobactin; MetaCyc:CPD-9985; Declared type: small_molecule small molecule petrobactin siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne]; NCBIProtein:AAT54153.1; Declared type: protein protein siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne] siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne]; NCBIProtein:AAT54154.1; Declared type: protein protein siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne] AMP-binding protein [Bacillus anthracis str. Sterne]; NCBIProtein:AAT54155.1; Declared type: protein protein AMP-binding protein [Bacillus anthracis str. Sterne] hypothetical protein BAS1841 [Bacillus anthracis str. Sterne]; NCBIProtein:AAT54156.1; Declared type: protein protein hypothetical protein BAS1841 [Bacillus anthracis str. Sterne] hypothetical protein BAS1842 [Bacillus anthracis str. Sterne]; NCBIProtein:AAT54157.1; Declared type: protein protein hypothetical protein BAS1842 [Bacillus anthracis str. Sterne] conserved hypothetical protein [Bacillus anthracis str. Sterne]; NCBIProtein:AAT54158.1; Declared type: protein protein conserved hypothetical protein [Bacillus anthracis str. Sterne] 3-dehydroshikimate dehydratase; MetaCyc:CPLX-7874; Declared type: complex complex 3-dehydroshikimate dehydratase manganese(2+); CHEBI:29035; Declared type: cofactor cofactor manganese(2+) Carrier phosphopantetheinylation; RHEA:48405; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity Carrier phosphopantetheinylation 3-dehydroshikimate dehydration; RHEA:24849; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity 3-dehydroshikimate dehydration 3,4-dihydroxybenzoate adenylation; RHEA:62469; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity 3,4-dihydroxybenzoate adenylation Aryl-carrier loading; RHEA:62477; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity Aryl-carrier loading Initial citrate-spermidine ligation; RHEA:63809; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity Initial citrate-spermidine ligation Second ligation before aromatic transfer; RHEA:63813; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity Second ligation before aromatic transfer Aromatic transfer before second ligation; RHEA:64029; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity Aromatic transfer before second ligation Second ligation after aromatic transfer; RHEA:63817; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity Second ligation after aromatic transfer Aromatic transfer on citryl-bis(spermidine); RHEA:64025; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity Aromatic transfer on citryl- bis(spermidine) Terminal petrobactin aryl transfer; MetaCyc:RXN-10679; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity Terminal petrobactin aryl transfer Bacillus anthracis str. Sterne; NCBITaxon:260799; Declared type: taxon taxon Bacillus anthracis str. Sterne petrobactin biosynthesis; MetaCyc:PWY-6289; Declared type: pathway pathway petrobactin biosynthesis

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Bacillus anthracis str. Sterne
NCBITaxon:260799		taxon
apo-[aryl-carrier protein]
RHEA-COMP:17557		chemical entity
coenzyme A(4-)
CHEBI:57287		small molecule
holo-[aryl-carrier protein]
RHEA-COMP:15903		chemical entity
adenosine 3',5'-bismonophosphate(4-)
CHEBI:58343		small molecule
hydron
CHEBI:15378		small molecule
3-dehydroshikimate
CHEBI:16630		small molecule
3,4-dihydroxybenzoate
CHEBI:36241		small molecule
water
CHEBI:15377		small molecule
ATP(4-)
CHEBI:30616		small molecule
3,4-dihydroxybenzoyl-AMP(1-)
CHEBI:144942		small molecule
diphosphate(3-)
CHEBI:33019		small molecule
3,4-dihydroxybenzoyl-[aryl-carrier protein]
RHEA-COMP:15941		chemical entity
adenosine 5'-monophosphate(2-)
CHEBI:456215		small molecule
spermidine(3+)
CHEBI:57834		small molecule
citrate(3-)
CHEBI:16947		small molecule
N-citryl-spermidine zwitterion
CHEBI:149586		small molecule
N(8),N'(8)-citryl-bis(spermidine) (+3)
CHEBI:149592		small molecule
N(1)-(3,4-dihydroxybenzoyl)-N(8)-citryl-spermidine (1-)
CHEBI:149593		small molecule
N(1)-(3,4-dihydroxybenzoyl)-N(8),N'(8)-citryl-bis(spermidine) (2+)
CHEBI:149594		small molecule
petrobactin
MetaCyc:CPD-9985		small molecule
siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54153.1		protein
siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54154.1		protein
AMP-binding protein [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54155.1		protein
hypothetical protein BAS1841 [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54156.1		protein
hypothetical protein BAS1842 [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54157.1		protein
conserved hypothetical protein [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54158.1		protein
3-dehydroshikimate dehydratase
MetaCyc:CPLX-7874		complex
manganese(2+)
CHEBI:29035		cofactor
Carrier phosphopantetheinylation
RHEA:48405		molecular activityleft to right
3-dehydroshikimate dehydration
RHEA:24849		molecular activityleft to right
3,4-dihydroxybenzoate adenylation
RHEA:62469		molecular activityleft to right
Aryl-carrier loading
RHEA:62477		molecular activityleft to right
Initial citrate-spermidine ligation
RHEA:63809		molecular activityleft to right
Second ligation before aromatic transfer
RHEA:63813		molecular activityleft to right
Aromatic transfer before second ligation
RHEA:64029		molecular activityleft to right
Second ligation after aromatic transfer
RHEA:63817		molecular activityleft to right
Aromatic transfer on citryl-bis(spermidine)
RHEA:64025		molecular activityleft to right
Terminal petrobactin aryl transfer
MetaCyc:RXN-10679		molecular activityleft to right

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
apo-[aryl-carrier protein]
RHEA-COMP:17557		consumes

This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

Carrier phosphopantetheinylation
RHEA:48405
  • RHEA:48405

    Source assertion: RHEA:48405 assigns RHEA-COMP:17557 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/48405 rh:substrates http://rdf.rhea-db.org/48404_L; http://rdf.rhea-db.org/48404_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_48404_compound_17557; rh:compound http://rdf.rhea-db.org/Compound_17557; rh:name and rh:reactivePart
coenzyme A(4-)
CHEBI:57287		consumes

Carrier phosphopantetheinylation
RHEA:48405
  • RHEA:48405

    Source assertion: RHEA:48405 assigns CHEBI:57287 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/48405 rh:substrates http://rdf.rhea-db.org/48404_L; http://rdf.rhea-db.org/48404_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_48404_compound_1264; rh:compound http://rdf.rhea-db.org/Compound_1264; rh:chebi
Carrier phosphopantetheinylation
RHEA:48405		produces

This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

holo-[aryl-carrier protein]
RHEA-COMP:15903
  • RHEA:48405

    Source assertion: RHEA:48405 assigns RHEA-COMP:15903 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/48405 rh:products http://rdf.rhea-db.org/48404_R; http://rdf.rhea-db.org/48404_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_48404_compound_15903; rh:compound http://rdf.rhea-db.org/Compound_15903; rh:name and rh:reactivePart
Carrier phosphopantetheinylation
RHEA:48405		produces

adenosine 3',5'-bismonophosphate(4-)
CHEBI:58343
  • RHEA:48405

    Source assertion: RHEA:48405 assigns CHEBI:58343 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/48405 rh:products http://rdf.rhea-db.org/48404_R; http://rdf.rhea-db.org/48404_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_48404_compound_1731; rh:compound http://rdf.rhea-db.org/Compound_1731; rh:chebi
Carrier phosphopantetheinylation
RHEA:48405		produces

hydron
CHEBI:15378
  • RHEA:48405

    Source assertion: RHEA:48405 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/48405 rh:products http://rdf.rhea-db.org/48404_R; http://rdf.rhea-db.org/48404_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_48404_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249; rh:chebi
3-dehydroshikimate
CHEBI:16630		consumes

3-dehydroshikimate dehydration
RHEA:24849
  • RHEA:24849

    Source assertion: RHEA:24849 assigns CHEBI:16630 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/24849 rh:substrates http://rdf.rhea-db.org/24848_L; http://rdf.rhea-db.org/24848_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_24848_compound_3489; rh:compound http://rdf.rhea-db.org/Compound_3489; rh:chebi
3-dehydroshikimate dehydration
RHEA:24849		produces

3,4-dihydroxybenzoate
CHEBI:36241
  • RHEA:24849

    Source assertion: RHEA:24849 assigns CHEBI:36241 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/24849 rh:products http://rdf.rhea-db.org/24848_R; http://rdf.rhea-db.org/24848_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_24848_compound_3099; rh:compound http://rdf.rhea-db.org/Compound_3099; rh:chebi
3-dehydroshikimate dehydration
RHEA:24849		produces

water
CHEBI:15377
  • RHEA:24849

    Source assertion: RHEA:24849 assigns CHEBI:15377 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/24849 rh:products http://rdf.rhea-db.org/24848_R; http://rdf.rhea-db.org/24848_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_24848_compound_1283; rh:compound http://rdf.rhea-db.org/Compound_1283; rh:chebi
3,4-dihydroxybenzoate
CHEBI:36241		consumes

3,4-dihydroxybenzoate adenylation
RHEA:62469
  • RHEA:62469

    Source assertion: RHEA:62469 assigns CHEBI:36241 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62469 rh:substrates http://rdf.rhea-db.org/62468_L; http://rdf.rhea-db.org/62468_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62468_compound_3099; rh:compound http://rdf.rhea-db.org/Compound_3099; rh:chebi
ATP(4-)
CHEBI:30616		consumes

3,4-dihydroxybenzoate adenylation
RHEA:62469
  • RHEA:62469

    Source assertion: RHEA:62469 assigns CHEBI:30616 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62469 rh:substrates http://rdf.rhea-db.org/62468_L; http://rdf.rhea-db.org/62468_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62468_compound_6372; rh:compound http://rdf.rhea-db.org/Compound_6372; rh:chebi
hydron
CHEBI:15378		consumes

3,4-dihydroxybenzoate adenylation
RHEA:62469
  • RHEA:62469

    Source assertion: RHEA:62469 assigns CHEBI:15378 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62469 rh:substrates http://rdf.rhea-db.org/62468_L; http://rdf.rhea-db.org/62468_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62468_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249; rh:chebi
3,4-dihydroxybenzoate adenylation
RHEA:62469		produces

3,4-dihydroxybenzoyl-AMP(1-)
CHEBI:144942
  • RHEA:62469

    Source assertion: RHEA:62469 assigns CHEBI:144942 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62469 rh:products http://rdf.rhea-db.org/62468_R; http://rdf.rhea-db.org/62468_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62468_compound_16102; rh:compound http://rdf.rhea-db.org/Compound_16102; rh:chebi
3,4-dihydroxybenzoate adenylation
RHEA:62469		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:62469

    Source assertion: RHEA:62469 assigns CHEBI:33019 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62469 rh:products http://rdf.rhea-db.org/62468_R; http://rdf.rhea-db.org/62468_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62468_compound_2106; rh:compound http://rdf.rhea-db.org/Compound_2106; rh:chebi
3,4-dihydroxybenzoyl-AMP(1-)
CHEBI:144942		consumes

Aryl-carrier loading
RHEA:62477
  • RHEA:62477

    Source assertion: RHEA:62477 assigns CHEBI:144942 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:substrates http://rdf.rhea-db.org/62476_L; http://rdf.rhea-db.org/62476_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_16102; rh:compound http://rdf.rhea-db.org/Compound_16102; rh:chebi
holo-[aryl-carrier protein]
RHEA-COMP:15903		consumes

This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

Aryl-carrier loading
RHEA:62477
  • RHEA:62477

    Source assertion: RHEA:62477 assigns RHEA-COMP:15903 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:substrates http://rdf.rhea-db.org/62476_L; http://rdf.rhea-db.org/62476_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_15903; rh:compound http://rdf.rhea-db.org/Compound_15903; rh:name and rh:reactivePart
Aryl-carrier loading
RHEA:62477		produces

This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

3,4-dihydroxybenzoyl-[aryl-carrier protein]
RHEA-COMP:15941
  • RHEA:62477

    Source assertion: RHEA:62477 assigns RHEA-COMP:15941 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:products http://rdf.rhea-db.org/62476_R; http://rdf.rhea-db.org/62476_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_15941; rh:compound http://rdf.rhea-db.org/Compound_15941; rh:name and rh:reactivePart
Aryl-carrier loading
RHEA:62477		produces

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • RHEA:62477

    Source assertion: RHEA:62477 assigns CHEBI:456215 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:products http://rdf.rhea-db.org/62476_R; http://rdf.rhea-db.org/62476_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_2590; rh:compound http://rdf.rhea-db.org/Compound_2590; rh:chebi
Aryl-carrier loading
RHEA:62477		produces

hydron
CHEBI:15378
  • RHEA:62477

    Source assertion: RHEA:62477 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:products http://rdf.rhea-db.org/62476_R; http://rdf.rhea-db.org/62476_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249; rh:chebi
spermidine(3+)
CHEBI:57834		consumes

Initial citrate-spermidine ligation
RHEA:63809
  • RHEA:63809

    Source assertion: RHEA:63809 assigns CHEBI:57834 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63809 rh:substrates http://rdf.rhea-db.org/63808_L; http://rdf.rhea-db.org/63808_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63808_compound_2557; rh:compound http://rdf.rhea-db.org/Compound_2557; rh:chebi
citrate(3-)
CHEBI:16947		consumes

Initial citrate-spermidine ligation
RHEA:63809
  • RHEA:63809

    Source assertion: RHEA:63809 assigns CHEBI:16947 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63809 rh:substrates http://rdf.rhea-db.org/63808_L; http://rdf.rhea-db.org/63808_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63808_compound_5244; rh:compound http://rdf.rhea-db.org/Compound_5244; rh:chebi
ATP(4-)
CHEBI:30616		consumes

Initial citrate-spermidine ligation
RHEA:63809
  • RHEA:63809

    Source assertion: RHEA:63809 assigns CHEBI:30616 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63809 rh:substrates http://rdf.rhea-db.org/63808_L; http://rdf.rhea-db.org/63808_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63808_compound_6372; rh:compound http://rdf.rhea-db.org/Compound_6372; rh:chebi
Initial citrate-spermidine ligation
RHEA:63809		produces

The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.

N-citryl-spermidine zwitterion
CHEBI:149586
  • RHEA:63809

    Source assertion: RHEA:63809 assigns CHEBI:149586 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63809 rh:products http://rdf.rhea-db.org/63808_R; http://rdf.rhea-db.org/63808_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63808_compound_16439; rh:compound http://rdf.rhea-db.org/Compound_16439; rh:chebi
Initial citrate-spermidine ligation
RHEA:63809		produces

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • RHEA:63809

    Source assertion: RHEA:63809 assigns CHEBI:456215 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63809 rh:products http://rdf.rhea-db.org/63808_R; http://rdf.rhea-db.org/63808_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63808_compound_2590; rh:compound http://rdf.rhea-db.org/Compound_2590; rh:chebi
Initial citrate-spermidine ligation
RHEA:63809		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:63809

    Source assertion: RHEA:63809 assigns CHEBI:33019 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63809 rh:products http://rdf.rhea-db.org/63808_R; http://rdf.rhea-db.org/63808_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63808_compound_2106; rh:compound http://rdf.rhea-db.org/Compound_2106; rh:chebi
Initial citrate-spermidine ligation
RHEA:63809		produces

hydron
CHEBI:15378
  • RHEA:63809

    Source assertion: RHEA:63809 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63809 rh:products http://rdf.rhea-db.org/63808_R; http://rdf.rhea-db.org/63808_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63808_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249; rh:chebi
N-citryl-spermidine zwitterion
CHEBI:149586		consumes

The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.

Second ligation before aromatic transfer
RHEA:63813
  • RHEA:63813

    Source assertion: RHEA:63813 assigns CHEBI:149586 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63813 rh:substrates http://rdf.rhea-db.org/63812_L; http://rdf.rhea-db.org/63812_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63812_compound_16439; rh:compound http://rdf.rhea-db.org/Compound_16439; rh:chebi
spermidine(3+)
CHEBI:57834		consumes

Second ligation before aromatic transfer
RHEA:63813
  • RHEA:63813

    Source assertion: RHEA:63813 assigns CHEBI:57834 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63813 rh:substrates http://rdf.rhea-db.org/63812_L; http://rdf.rhea-db.org/63812_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63812_compound_2557; rh:compound http://rdf.rhea-db.org/Compound_2557; rh:chebi
ATP(4-)
CHEBI:30616		consumes

Second ligation before aromatic transfer
RHEA:63813
  • RHEA:63813

    Source assertion: RHEA:63813 assigns CHEBI:30616 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63813 rh:substrates http://rdf.rhea-db.org/63812_L; http://rdf.rhea-db.org/63812_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63812_compound_6372; rh:compound http://rdf.rhea-db.org/Compound_6372; rh:chebi
Second ligation before aromatic transfer
RHEA:63813		produces

N(8),N'(8)-citryl-bis(spermidine) (+3)
CHEBI:149592
  • RHEA:63813

    Source assertion: RHEA:63813 assigns CHEBI:149592 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63813 rh:products http://rdf.rhea-db.org/63812_R; http://rdf.rhea-db.org/63812_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63812_compound_16440; rh:compound http://rdf.rhea-db.org/Compound_16440; rh:chebi
Second ligation before aromatic transfer
RHEA:63813		produces

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • RHEA:63813

    Source assertion: RHEA:63813 assigns CHEBI:456215 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63813 rh:products http://rdf.rhea-db.org/63812_R; http://rdf.rhea-db.org/63812_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63812_compound_2590; rh:compound http://rdf.rhea-db.org/Compound_2590; rh:chebi
Second ligation before aromatic transfer
RHEA:63813		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:63813

    Source assertion: RHEA:63813 assigns CHEBI:33019 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63813 rh:products http://rdf.rhea-db.org/63812_R; http://rdf.rhea-db.org/63812_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63812_compound_2106; rh:compound http://rdf.rhea-db.org/Compound_2106; rh:chebi
Second ligation before aromatic transfer
RHEA:63813		produces

hydron
CHEBI:15378
  • RHEA:63813

    Source assertion: RHEA:63813 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63813 rh:products http://rdf.rhea-db.org/63812_R; http://rdf.rhea-db.org/63812_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63812_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249; rh:chebi
N-citryl-spermidine zwitterion
CHEBI:149586		consumes

The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.

Aromatic transfer before second ligation
RHEA:64029
  • RHEA:64029

    Source assertion: RHEA:64029 assigns CHEBI:149586 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64029 rh:substrates http://rdf.rhea-db.org/64028_L; http://rdf.rhea-db.org/64028_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64028_compound_16439; rh:compound http://rdf.rhea-db.org/Compound_16439; rh:chebi
3,4-dihydroxybenzoyl-[aryl-carrier protein]
RHEA-COMP:15941		consumes

This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

Aromatic transfer before second ligation
RHEA:64029
  • RHEA:64029

    Source assertion: RHEA:64029 assigns RHEA-COMP:15941 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64029 rh:substrates http://rdf.rhea-db.org/64028_L; http://rdf.rhea-db.org/64028_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64028_compound_15941; rh:compound http://rdf.rhea-db.org/Compound_15941; rh:name and rh:reactivePart
Aromatic transfer before second ligation
RHEA:64029		produces

The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.

N(1)-(3,4-dihydroxybenzoyl)-N(8)-citryl-spermidine (1-)
CHEBI:149593
  • RHEA:64029

    Source assertion: RHEA:64029 assigns CHEBI:149593 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64029 rh:products http://rdf.rhea-db.org/64028_R; http://rdf.rhea-db.org/64028_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64028_compound_16442; rh:compound http://rdf.rhea-db.org/Compound_16442; rh:chebi
Aromatic transfer before second ligation
RHEA:64029		produces

This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

holo-[aryl-carrier protein]
RHEA-COMP:15903
  • RHEA:64029

    Source assertion: RHEA:64029 assigns RHEA-COMP:15903 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64029 rh:products http://rdf.rhea-db.org/64028_R; http://rdf.rhea-db.org/64028_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64028_compound_15903; rh:compound http://rdf.rhea-db.org/Compound_15903; rh:name and rh:reactivePart
Aromatic transfer before second ligation
RHEA:64029		produces

hydron
CHEBI:15378
  • RHEA:64029

    Source assertion: RHEA:64029 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64029 rh:products http://rdf.rhea-db.org/64028_R; http://rdf.rhea-db.org/64028_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64028_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249; rh:chebi
N(1)-(3,4-dihydroxybenzoyl)-N(8)-citryl-spermidine (1-)
CHEBI:149593		consumes

The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.

Second ligation after aromatic transfer
RHEA:63817
  • RHEA:63817

    Source assertion: RHEA:63817 assigns CHEBI:149593 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63817 rh:substrates http://rdf.rhea-db.org/63816_L; http://rdf.rhea-db.org/63816_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63816_compound_16442; rh:compound http://rdf.rhea-db.org/Compound_16442; rh:chebi
spermidine(3+)
CHEBI:57834		consumes

Second ligation after aromatic transfer
RHEA:63817
  • RHEA:63817

    Source assertion: RHEA:63817 assigns CHEBI:57834 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63817 rh:substrates http://rdf.rhea-db.org/63816_L; http://rdf.rhea-db.org/63816_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63816_compound_2557; rh:compound http://rdf.rhea-db.org/Compound_2557; rh:chebi
ATP(4-)
CHEBI:30616		consumes

Second ligation after aromatic transfer
RHEA:63817
  • RHEA:63817

    Source assertion: RHEA:63817 assigns CHEBI:30616 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63817 rh:substrates http://rdf.rhea-db.org/63816_L; http://rdf.rhea-db.org/63816_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63816_compound_6372; rh:compound http://rdf.rhea-db.org/Compound_6372; rh:chebi
Second ligation after aromatic transfer
RHEA:63817		produces

The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.

N(1)-(3,4-dihydroxybenzoyl)-N(8),N'(8)-citryl-bis(spermidine) (2+)
CHEBI:149594
  • RHEA:63817

    Source assertion: RHEA:63817 assigns CHEBI:149594 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63817 rh:products http://rdf.rhea-db.org/63816_R; http://rdf.rhea-db.org/63816_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63816_compound_16441; rh:compound http://rdf.rhea-db.org/Compound_16441; rh:chebi
Second ligation after aromatic transfer
RHEA:63817		produces

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • RHEA:63817

    Source assertion: RHEA:63817 assigns CHEBI:456215 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63817 rh:products http://rdf.rhea-db.org/63816_R; http://rdf.rhea-db.org/63816_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63816_compound_2590; rh:compound http://rdf.rhea-db.org/Compound_2590; rh:chebi
Second ligation after aromatic transfer
RHEA:63817		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:63817

    Source assertion: RHEA:63817 assigns CHEBI:33019 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63817 rh:products http://rdf.rhea-db.org/63816_R; http://rdf.rhea-db.org/63816_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63816_compound_2106; rh:compound http://rdf.rhea-db.org/Compound_2106; rh:chebi
Second ligation after aromatic transfer
RHEA:63817		produces

hydron
CHEBI:15378
  • RHEA:63817

    Source assertion: RHEA:63817 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63817 rh:products http://rdf.rhea-db.org/63816_R; http://rdf.rhea-db.org/63816_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63816_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249; rh:chebi
N(8),N'(8)-citryl-bis(spermidine) (+3)
CHEBI:149592		consumes

Aromatic transfer on citryl-bis(spermidine)
RHEA:64025
  • RHEA:64025

    Source assertion: RHEA:64025 assigns CHEBI:149592 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64025 rh:substrates http://rdf.rhea-db.org/64024_L; http://rdf.rhea-db.org/64024_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64024_compound_16440; rh:compound http://rdf.rhea-db.org/Compound_16440; rh:chebi
3,4-dihydroxybenzoyl-[aryl-carrier protein]
RHEA-COMP:15941		consumes

This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

Aromatic transfer on citryl-bis(spermidine)
RHEA:64025
  • RHEA:64025

    Source assertion: RHEA:64025 assigns RHEA-COMP:15941 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64025 rh:substrates http://rdf.rhea-db.org/64024_L; http://rdf.rhea-db.org/64024_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64024_compound_15941; rh:compound http://rdf.rhea-db.org/Compound_15941; rh:name and rh:reactivePart
Aromatic transfer on citryl-bis(spermidine)
RHEA:64025		produces

The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.

N(1)-(3,4-dihydroxybenzoyl)-N(8),N'(8)-citryl-bis(spermidine) (2+)
CHEBI:149594
  • RHEA:64025

    Source assertion: RHEA:64025 assigns CHEBI:149594 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64025 rh:products http://rdf.rhea-db.org/64024_R; http://rdf.rhea-db.org/64024_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64024_compound_16441; rh:compound http://rdf.rhea-db.org/Compound_16441; rh:chebi
Aromatic transfer on citryl-bis(spermidine)
RHEA:64025		produces

This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

holo-[aryl-carrier protein]
RHEA-COMP:15903
  • RHEA:64025

    Source assertion: RHEA:64025 assigns RHEA-COMP:15903 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64025 rh:products http://rdf.rhea-db.org/64024_R; http://rdf.rhea-db.org/64024_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64024_compound_15903; rh:compound http://rdf.rhea-db.org/Compound_15903; rh:name and rh:reactivePart
Aromatic transfer on citryl-bis(spermidine)
RHEA:64025		produces

hydron
CHEBI:15378
  • RHEA:64025

    Source assertion: RHEA:64025 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64025 rh:products http://rdf.rhea-db.org/64024_R; http://rdf.rhea-db.org/64024_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64024_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249; rh:chebi
N(1)-(3,4-dihydroxybenzoyl)-N(8),N'(8)-citryl-bis(spermidine) (2+)
CHEBI:149594		consumes

The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only. The issued native/ChEBI intermediate does not specify absolute citrate-derived stereochemistry. The graph preserves that class and does not assert racemic physiology or acceptance of all stereoisomers.

Terminal petrobactin aryl transfer
MetaCyc:RXN-10679
  • MetaCyc:RXN-10679

    Source assertion: The native terminal equation places CPD-11481 on its left side with unit coefficient and physiological left-to-right direction.

    Source locationReaction[@frameid="RXN-10679"]/left/*[@frameid="CPD-11481"]; reaction-direction
  • MetaCyc:CPD-11481

    Source assertion: The native compound supplies its own InChI, formula C27H48N6O8 and charge +2; the native reaction does not supply a ChEBI identifier.

    Source locationCompound[@frameid="CPD-11481"]/inchi; cml/molecule
  • CHEBI:149594

    Source assertion: The independently issued chemical has formula C27H48N6O8, charge +2 and the same InChI as the native terminal substrate. This bridge is checked independently of the rejected terminal product mapping.

    Source locationstatements[subject=CHEBI:149594,predicate=chemrof:inchi_string|chemrof:generalized_empirical_formula|chemrof:charge]
3,4-dihydroxybenzoyl-[aryl-carrier protein]
RHEA-COMP:15941		consumes

The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

Terminal petrobactin aryl transfer
MetaCyc:RXN-10679
  • MetaCyc:RXN-10679

    Source assertion: The native terminal equation places 34-DHB-AsbD-Proteins on its left side with unit coefficient and physiological left-to-right direction.

    Source locationReaction[@frameid="RXN-10679"]/left/*[@frameid="34-DHB-AsbD-Proteins"]; reaction-direction
  • RHEA:63821

    Source assertion: The compared current terminal reaction explicitly assigns RHEA-COMP:15941 to its substrates; this carrier assertion is retained independently of its rejected ChEBI product connectivity.

    Source locationhttp://rdf.rhea-db.org/63821 rh:substrates http://rdf.rhea-db.org/63820_L; http://rdf.rhea-db.org/63820_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63820_compound_15941; rh:compound http://rdf.rhea-db.org/Compound_15941
Terminal petrobactin aryl transfer
MetaCyc:RXN-10679		produces

The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only.

petrobactin
MetaCyc:CPD-9985
  • MetaCyc:RXN-10679

    Source assertion: The native terminal equation places CPD-9985 on its right side with unit coefficient and physiological left-to-right direction.

    Source locationReaction[@frameid="RXN-10679"]/right/*[@frameid="CPD-9985"]; reaction-direction
  • MetaCyc:CPD-9985

    Source assertion: The native InChI connects each aromatic amide to the central amine through three carbons and each citrate amide through four. Formula C34H51N6O11 and charge +1 alone do not distinguish the conflicting constitutional isomer.

    Source locationCompound[@frameid="CPD-9985"]/inchi; cml/molecule/@formalCharge; cml/molecule/formula
  • PMID:22408253

    Source assertion: Figure 1A/C depicts the native three-carbon aromatic-amide arms and four-carbon citrate-amide arms.

    Source locationPMC3346087 Figure 1A and 1C
  • CHEBI:142778

    Source assertion: The inspected ChEBI InChI instead has four-carbon aromatic-amide and three-carbon citrate-amide arms. This conflicting structure is not the product selected here.

    Source locationNative InChI field, connectivity layer /c
Terminal petrobactin aryl transfer
MetaCyc:RXN-10679		produces

The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

holo-[aryl-carrier protein]
RHEA-COMP:15903
  • MetaCyc:RXN-10679

    Source assertion: The native terminal equation places Aryl-Carrier-Proteins on its right side with unit coefficient and physiological left-to-right direction.

    Source locationReaction[@frameid="RXN-10679"]/right/*[@frameid="Aryl-Carrier-Proteins"]; reaction-direction
  • RHEA:63821

    Source assertion: The compared current terminal reaction explicitly assigns RHEA-COMP:15903 to its products; this carrier assertion is retained independently of its rejected ChEBI product connectivity.

    Source locationhttp://rdf.rhea-db.org/63821 rh:products http://rdf.rhea-db.org/63820_R; http://rdf.rhea-db.org/63820_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63820_compound_15903; rh:compound http://rdf.rhea-db.org/Compound_15903
  • MetaCyc:Aryl-Carrier-Proteins

    Source assertion: The native output is the broader aryl-carrier protein class; it is not asserted identical to the narrower Rhea holo state.

    Source locationProtein[@frameid="Aryl-Carrier-Proteins"]/parent; common-name
  • PMID:22408253

    Source assertion: Figure 1C depicts release of AsbD-SH after the terminal aromatic transfer, supporting the unloaded phosphopantetheine-bearing carrier state.

    Source locationPMC3346087 Figure 1C, terminal AsbE arrow
Terminal petrobactin aryl transfer
MetaCyc:RXN-10679		produces

The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only.

hydron
CHEBI:15378
  • MetaCyc:RXN-10679

    Source assertion: The native terminal equation places PROTON on its right side with unit coefficient and physiological left-to-right direction.

    Source locationReaction[@frameid="RXN-10679"]/right/*[@frameid="PROTON"]; reaction-direction
3-dehydroshikimate dehydratase
MetaCyc:CPLX-7874		catalyzes

The native species-level complex is applied to the experimentally sourced Sterne AsbF dimer. Exact tagged constructs are not equated to the unmodified gene product.

3-dehydroshikimate dehydration
RHEA:24849
  • MetaCyc:CPLX-7874

    Source assertion: The native dimer catalyzes DHSHIKIMATE-DEHYDRO-RXN and contains two copies of MONOMER-14940.

    Source locationProtein[@frameid="CPLX-7874"]/catalyzes; component/Protein; component/coefficient
  • PMID:18955706

    Source assertion: Purified AsbF converted 3-dehydroshikimate to 3,4-dihydroxybenzoate; structural and solution measurements support a dimer.

    Source locationPMC2579390 Results/sec3/p[2]; sec5/p[2]
AMP-binding protein [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54155.1		enables

AsbC gene-product role; the original Sterne translation equals the current reviewed sequence. The two native component transformations are retained without additionally counting the overlapping Rhea net reaction. Exact tagged constructs and a fixed oligomeric state are not established here.

3,4-dihydroxybenzoate adenylation
RHEA:62469
  • NCBIProtein:AAT54155.1

    Source assertion: AAT54155.1 is the original versioned Sterne gene product for the selected AsbC role; its source feature binds strain Sterne and taxon:260799. Native identity does not assert exact engineered-construct equality.

    Source locationGBSeq[GBSeq_accession-version="AAT54155.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals; GBSeq_sequence
  • UniProtKB:Q81RQ7

    Source assertion: The reviewed species-level AsbC entry assigns the corresponding biochemical activity with ECO:0000269 citations. Its source annotation is used with the separately checked Sterne sequence correspondence, not as exact tagged-construct identity.

    Source location/comments/2/reaction/name; reactionCrossReferences; evidences
  • PMID:17346033

    Source assertion: The inspected abstract reports AsbC adenylation of 3,4-dihydroxybenzoate and subsequent transfer to AsbD. The complete original paper was not available.

    Source locationresultList/result[id="17346033"]/abstractText
AMP-binding protein [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54155.1		enables

AsbC gene-product role; the original Sterne translation equals the current reviewed sequence. The two native component transformations are retained without additionally counting the overlapping Rhea net reaction. Exact tagged constructs and a fixed oligomeric state are not established here.

Aryl-carrier loading
RHEA:62477
  • NCBIProtein:AAT54155.1

    Source assertion: AAT54155.1 is the original versioned Sterne gene product for the selected AsbC role; its source feature binds strain Sterne and taxon:260799. Native identity does not assert exact engineered-construct equality.

    Source locationGBSeq[GBSeq_accession-version="AAT54155.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals; GBSeq_sequence
  • UniProtKB:Q81RQ7

    Source assertion: The reviewed species-level AsbC entry assigns the corresponding biochemical activity with ECO:0000269 citations. Its source annotation is used with the separately checked Sterne sequence correspondence, not as exact tagged-construct identity.

    Source location/comments/3/reaction/name; reactionCrossReferences; evidences
  • PMID:17346033

    Source assertion: The inspected abstract reports AsbC adenylation of 3,4-dihydroxybenzoate and subsequent transfer to AsbD. The complete original paper was not available.

    Source locationresultList/result[id="17346033"]/abstractText
siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54153.1		enables

AsbA gene-product activity does not assert monomer sufficiency. The original Sterne translation equals the current reviewed sequence. Magnesium-dependent assay context is retained without an independently established metal-binding cofactor relation. AsbB can partly substitute in vitro, so this edge does not assert universal AsbA indispensability.

Initial citrate-spermidine ligation
RHEA:63809
  • NCBIProtein:AAT54153.1

    Source assertion: AAT54153.1 is the original versioned Sterne gene product for the selected AsbA role; its source feature binds strain Sterne and taxon:260799. Native identity does not assert exact engineered-construct equality.

    Source locationGBSeq[GBSeq_accession-version="AAT54153.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals; GBSeq_sequence
  • UniProtKB:Q81RQ9

    Source assertion: The reviewed species-level AsbA entry assigns the corresponding biochemical activity with ECO:0000269 citations. Its source annotation is used with the separately checked Sterne sequence correspondence, not as exact tagged-construct identity.

    Source location/comments/1/reaction/name; reactionCrossReferences; evidences
  • PMID:22408253

    Source assertion: The Sterne-derived AsbA preparation supplied the first citrate-spermidine ligation; later AsbE transfer branches are supported in the same study.

    Source locationPMC3346087 Results/sec21/p[1]; sec22/p[3]; Figure 1C
siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54154.1		enables

The versioned Sterne AsbB translation is 610 residues; current reviewed Q81RQ8 has two additional N-terminal residues. The primary supports a dimeric preparation,; its gene-product enables relation does not establish monomer sufficiency. Exact recombinant construct equality is not established.

Second ligation before aromatic transfer
RHEA:63813
  • NCBIProtein:AAT54154.1

    Source assertion: AAT54154.1 is the original versioned Sterne gene product for the selected AsbB role; its source feature binds strain Sterne and taxon:260799. Native identity does not assert exact engineered-construct equality.

    Source locationGBSeq[GBSeq_accession-version="AAT54154.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals; GBSeq_sequence
  • UniProtKB:Q81RQ8

    Source assertion: The reviewed species-level AsbB entry assigns the corresponding biochemical activity with ECO:0000269 citations. Its source annotation is used with the separately checked Sterne sequence correspondence, not as exact tagged-construct identity.

    Source location/comments/1/reaction/name; reactionCrossReferences; evidences
  • PMID:22408253

    Source assertion: Sterne AsbB accepts the two citryl intermediates. Its homodimer is supported by crystallography and size exclusion.

    Source locationPMC3346087 Results/sec22/p[2-3]; sec23/p[3]; sec25/p[1]; Figure 1C
hypothetical protein BAS1842 [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54157.1		enables

The versioned Sterne AsbE translation is 331 residues; current reviewed Q81RQ5 matches its suffix after four residues. The role is grounded in the Sterne source and primary functional assignment, not full-sequence equality to the current annotation. Its fixed assembly is unresolved.

Aromatic transfer before second ligation
RHEA:64029
  • NCBIProtein:AAT54157.1

    Source assertion: AAT54157.1 is the original versioned Sterne gene product for the selected AsbE role; its source feature binds strain Sterne and taxon:260799. Native identity does not assert exact engineered-construct equality.

    Source locationGBSeq[GBSeq_accession-version="AAT54157.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals; GBSeq_sequence
  • UniProtKB:Q81RQ5

    Source assertion: The reviewed species-level AsbE entry assigns the corresponding biochemical activity with ECO:0000269 citations. Its source annotation is used with the separately checked Sterne sequence correspondence, not as exact tagged-construct identity.

    Source location/comments/1/reaction/name; reactionCrossReferences; evidences
  • PMID:22408253

    Source assertion: Sterne-derived AsbC/D/E preparations supported both aromatic-transfer branches and terminal product formation.

    Source locationPMC3346087 Results/sec21/p[1]; sec28/p[2-3]; Figure 1C and Figure 7
siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54154.1		enables

The versioned Sterne AsbB translation is 610 residues; current reviewed Q81RQ8 has two additional N-terminal residues. The primary supports a dimeric preparation,; its gene-product enables relation does not establish monomer sufficiency. Exact recombinant construct equality is not established.

Second ligation after aromatic transfer
RHEA:63817
  • NCBIProtein:AAT54154.1

    Source assertion: AAT54154.1 is the original versioned Sterne gene product for the selected AsbB role; its source feature binds strain Sterne and taxon:260799. Native identity does not assert exact engineered-construct equality.

    Source locationGBSeq[GBSeq_accession-version="AAT54154.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals; GBSeq_sequence
  • UniProtKB:Q81RQ8

    Source assertion: The reviewed species-level AsbB entry assigns the corresponding biochemical activity with ECO:0000269 citations. Its source annotation is used with the separately checked Sterne sequence correspondence, not as exact tagged-construct identity.

    Source location/comments/2/reaction/name; reactionCrossReferences; evidences
  • PMID:22408253

    Source assertion: Sterne AsbB accepts the two citryl intermediates. Its homodimer is supported by crystallography and size exclusion.

    Source locationPMC3346087 Results/sec22/p[2-3]; sec23/p[3]; sec25/p[1]; Figure 1C
hypothetical protein BAS1842 [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54157.1		enables

The versioned Sterne AsbE translation is 331 residues; current reviewed Q81RQ5 matches its suffix after four residues. The role is grounded in the Sterne source and primary functional assignment, not full-sequence equality to the current annotation. Its fixed assembly is unresolved. The bis(spermidine) branch uses the current database single-transfer step; the primary observed final product and proposed successive transfers because the monoaryl intermediate did not accumulate.

Aromatic transfer on citryl-bis(spermidine)
RHEA:64025
  • NCBIProtein:AAT54157.1

    Source assertion: AAT54157.1 is the original versioned Sterne gene product for the selected AsbE role; its source feature binds strain Sterne and taxon:260799. Native identity does not assert exact engineered-construct equality.

    Source locationGBSeq[GBSeq_accession-version="AAT54157.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals; GBSeq_sequence
  • UniProtKB:Q81RQ5

    Source assertion: The reviewed species-level AsbE entry assigns the corresponding biochemical activity with ECO:0000269 citations. Its source annotation is used with the separately checked Sterne sequence correspondence, not as exact tagged-construct identity.

    Source location/comments/2/reaction/name; reactionCrossReferences; evidences
  • PMID:22408253

    Source assertion: Sterne-derived AsbC/D/E preparations supported both aromatic-transfer branches and terminal product formation.

    Source locationPMC3346087 Results/sec21/p[1]; sec28/p[2-3]; Figure 1C and Figure 7
hypothetical protein BAS1842 [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54157.1		enables

The versioned Sterne AsbE translation is 331 residues; current reviewed Q81RQ5 matches its suffix after four residues. The role is grounded in the Sterne source and primary functional assignment, not full-sequence equality to the current annotation. Its fixed assembly is unresolved. The authentic native terminal product is retained despite the current ChEBI connectivity conflict.

Terminal petrobactin aryl transfer
MetaCyc:RXN-10679
  • NCBIProtein:AAT54157.1

    Source assertion: AAT54157.1 is the original versioned Sterne gene product for the selected AsbE role; its source feature binds strain Sterne and taxon:260799. Native identity does not assert exact engineered-construct equality.

    Source locationGBSeq[GBSeq_accession-version="AAT54157.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals; GBSeq_sequence
  • UniProtKB:Q81RQ5

    Source assertion: The reviewed species-level AsbE entry assigns the corresponding biochemical activity with ECO:0000269 citations. Its source annotation is used with the separately checked Sterne sequence correspondence, not as exact tagged-construct identity.

    Source location/comments/3/reaction/name; reactionCrossReferences; evidences
  • PMID:22408253

    Source assertion: Sterne-derived AsbC/D/E preparations supported both aromatic-transfer branches and terminal product formation.

    Source locationPMC3346087 Results/sec21/p[1]; sec28/p[2-3]; Figure 1C and Figure 7
Carrier phosphopantetheinylation
RHEA:48405		part_of

The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains RXN-19493 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="RXN-19493"]; reaction-layout[Reaction/@frameid="RXN-19493"]/direction
  • RHEA:48405

    Source assertion: Rhea master 48404 explicitly links directed reaction 48405; its independently inspected chemical sides implement the selected native transformation.

    Source locationhttp://rdf.rhea-db.org/48404 rh:directionalReaction http://rdf.rhea-db.org/48405; http://rdf.rhea-db.org/48405 rh:substrates/rh:products
3-dehydroshikimate dehydration
RHEA:24849		part_of

The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains DHSHIKIMATE-DEHYDRO-RXN and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="DHSHIKIMATE-DEHYDRO-RXN"]; reaction-layout[Reaction/@frameid="DHSHIKIMATE-DEHYDRO-RXN"]/direction
  • RHEA:24849

    Source assertion: Rhea master 24848 explicitly links directed reaction 24849; its independently inspected chemical sides implement the selected native transformation.

    Source locationhttp://rdf.rhea-db.org/24848 rh:directionalReaction http://rdf.rhea-db.org/24849; http://rdf.rhea-db.org/24849 rh:substrates/rh:products
3,4-dihydroxybenzoate adenylation
RHEA:62469		part_of

The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains RXN-10675 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="RXN-10675"]; reaction-layout[Reaction/@frameid="RXN-10675"]/direction
  • RHEA:62469

    Source assertion: Rhea master 62468 explicitly links directed reaction 62469; its independently inspected chemical sides implement the selected native transformation.

    Source locationhttp://rdf.rhea-db.org/62468 rh:directionalReaction http://rdf.rhea-db.org/62469; http://rdf.rhea-db.org/62469 rh:substrates/rh:products
Aryl-carrier loading
RHEA:62477		part_of

The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains RXN-10676 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="RXN-10676"]; reaction-layout[Reaction/@frameid="RXN-10676"]/direction
  • RHEA:62477

    Source assertion: Rhea master 62476 explicitly links directed reaction 62477; its independently inspected chemical sides implement the selected native transformation.

    Source locationhttp://rdf.rhea-db.org/62476 rh:directionalReaction http://rdf.rhea-db.org/62477; http://rdf.rhea-db.org/62477 rh:substrates/rh:products
Initial citrate-spermidine ligation
RHEA:63809		part_of

The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains RXN-10680 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="RXN-10680"]; reaction-layout[Reaction/@frameid="RXN-10680"]/direction
  • RHEA:63809

    Source assertion: Rhea master 63808 explicitly links directed reaction 63809; its independently inspected chemical sides implement the selected native transformation.

    Source locationhttp://rdf.rhea-db.org/63808 rh:directionalReaction http://rdf.rhea-db.org/63809; http://rdf.rhea-db.org/63809 rh:substrates/rh:products
Second ligation before aromatic transfer
RHEA:63813		part_of

The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains RXN-10681 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="RXN-10681"]; reaction-layout[Reaction/@frameid="RXN-10681"]/direction
  • RHEA:63813

    Source assertion: Rhea master 63812 explicitly links directed reaction 63813; its independently inspected chemical sides implement the selected native transformation.

    Source locationhttp://rdf.rhea-db.org/63812 rh:directionalReaction http://rdf.rhea-db.org/63813; http://rdf.rhea-db.org/63813 rh:substrates/rh:products
Aromatic transfer before second ligation
RHEA:64029		part_of

The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains RXN-10677 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="RXN-10677"]; reaction-layout[Reaction/@frameid="RXN-10677"]/direction
  • RHEA:64029

    Source assertion: Rhea master 64028 explicitly links directed reaction 64029; its independently inspected chemical sides implement the selected native transformation.

    Source locationhttp://rdf.rhea-db.org/64028 rh:directionalReaction http://rdf.rhea-db.org/64029; http://rdf.rhea-db.org/64029 rh:substrates/rh:products
Second ligation after aromatic transfer
RHEA:63817		part_of

The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains RXN-10678 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="RXN-10678"]; reaction-layout[Reaction/@frameid="RXN-10678"]/direction
  • RHEA:63817

    Source assertion: Rhea master 63816 explicitly links directed reaction 63817; its independently inspected chemical sides implement the selected native transformation.

    Source locationhttp://rdf.rhea-db.org/63816 rh:directionalReaction http://rdf.rhea-db.org/63817; http://rdf.rhea-db.org/63817 rh:substrates/rh:products
Aromatic transfer on citryl-bis(spermidine)
RHEA:64025		part_of

The original native object and current reaction are bridged by separately inspected chemical structures and carrier-class definitions; no absent native Rhea cross-reference is invented.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains RXN-10682 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="RXN-10682"]; reaction-layout[Reaction/@frameid="RXN-10682"]/direction
  • RHEA:64025

    Source assertion: Rhea master 64024 explicitly links directed reaction 64025; its independently inspected chemical sides implement the selected native transformation.

    Source locationhttp://rdf.rhea-db.org/64024 rh:directionalReaction http://rdf.rhea-db.org/64025; http://rdf.rhea-db.org/64025 rh:substrates/rh:products
Terminal petrobactin aryl transfer
MetaCyc:RXN-10679		part_of

The authentic native reaction and CPD-9985 product are selected. Native and ChEBI:142778 have equal formula/charge but different spermidine-arm connectivity; no exact equation or product equivalence to RHEA:63821 is asserted. Its independently specified carrier side supports the holo output only.

MetaCyc:PWY-6289
  • MetaCyc:PWY-6289

    Source assertion: The native pathway explicitly contains RXN-10679 and selects L2R in its reaction layout.

    Source locationPathway[@frameid="PWY-6289"]/reaction-list/Reaction[@frameid="RXN-10679"]; reaction-layout[Reaction/@frameid="RXN-10679"]/direction
  • MetaCyc:RXN-10679

    Source assertion: The authentic native reaction selects physiological left-to-right terminal transfer to CPD-9985.

    Source locationReaction[@frameid="RXN-10679"]/reaction-direction; left; right
siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54153.1		part_of

Bacillus anthracis str. Sterne
NCBITaxon:260799
  • NCBIProtein:AAT54153.1

    Source assertion: The source feature explicitly records strain Sterne and taxon:260799.

    Source locationGBSeq[GBSeq_accession-version="AAT54153.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals
siderophore biosynthesis protein, putative [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54154.1		part_of

Bacillus anthracis str. Sterne
NCBITaxon:260799
  • NCBIProtein:AAT54154.1

    Source assertion: The source feature explicitly records strain Sterne and taxon:260799.

    Source locationGBSeq[GBSeq_accession-version="AAT54154.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals
AMP-binding protein [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54155.1		part_of

Bacillus anthracis str. Sterne
NCBITaxon:260799
  • NCBIProtein:AAT54155.1

    Source assertion: The source feature explicitly records strain Sterne and taxon:260799.

    Source locationGBSeq[GBSeq_accession-version="AAT54155.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals
hypothetical protein BAS1841 [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54156.1		part_of

Bacillus anthracis str. Sterne
NCBITaxon:260799
  • NCBIProtein:AAT54156.1

    Source assertion: The source feature explicitly records strain Sterne and taxon:260799.

    Source locationGBSeq[GBSeq_accession-version="AAT54156.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals
hypothetical protein BAS1842 [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54157.1		part_of

Bacillus anthracis str. Sterne
NCBITaxon:260799
  • NCBIProtein:AAT54157.1

    Source assertion: The source feature explicitly records strain Sterne and taxon:260799.

    Source locationGBSeq[GBSeq_accession-version="AAT54157.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals
conserved hypothetical protein [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54158.1		part_of

Bacillus anthracis str. Sterne
NCBITaxon:260799
  • NCBIProtein:AAT54158.1

    Source assertion: The source feature explicitly records strain Sterne and taxon:260799.

    Source locationGBSeq[GBSeq_accession-version="AAT54158.1"]/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals
3-dehydroshikimate dehydratase
MetaCyc:CPLX-7874		has_part

Two AsbF subunits per source-defined dimer. The source Sterne translation and current reviewed Q81RQ4 sequence compare exactly; generic carrier-state counts elsewhere are not subunit counts.

conserved hypothetical protein [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54158.1
  • MetaCyc:CPLX-7874

    Source assertion: The native complex component is MONOMER-14940 with coefficient 2.

    Source locationProtein[@frameid="CPLX-7874"]/component/Protein; component/coefficient
  • MetaCyc:MONOMER-14940

    Source assertion: The native AsbF monomer cross-references UniProt Q81RQ4.

    Source locationProtein[@frameid="MONOMER-14940"]/dblink[dblink-db="UNIPROT"]/dblink-oid
  • NCBIProtein:AAT54158.1

    Source assertion: The original entry supplies the versioned Sterne AsbF sequence used in the independent comparison; it does not identify every experimental tag.

    Source locationGBSeq[GBSeq_accession-version="AAT54158.1"]/GBSeq_sequence
  • UniProtKB:Q81RQ4

    Source assertion: The reviewed full sequence supplies the independent exact comparison for the native AsbF component.

    Source location/primaryAccession; /sequence/value
  • PMID:18955706
    also supports a dimeric state of the enzyme in solution
    Source locationPMC2579390 Results/sec5/p[2]
3-dehydroshikimate dehydratase
MetaCyc:CPLX-7874		has_cofactor

Manganese is the experimentally preferred bound metal in the inspected preparation; the edge does not assert exclusive physiological metal use.

manganese(2+)
CHEBI:29035
  • UniProtKB:Q81RQ4

    Source assertion: The reviewed COFACTOR comment assigns Mn(2+) with ECO:0000269, citing PMID:18955706.

    Source location/comments/2/cofactors; /comments/2/note
  • PMID:18955706

    Source assertion: Metal rescue and structural analysis support manganese binding; other divalent metals can reactivate the preparation.

    Source locationPMC2579390 Results/sec4/p[1]; sec5/p[2]
hypothetical protein BAS1841 [Bacillus anthracis str. Sterne]
NCBIProtein:AAT54156.1		part_of

The selected versioned Sterne AsbD protein participates in petrobactin biosynthesis. This contextual participation does not assert an exact identity between AsbD and every member of the generic carrier classes.

MetaCyc:PWY-6289
  • NCBIProtein:AAT54156.1

    Source assertion: The versioned Sterne carrier protein supplies the selected AsbD gene-product identity.

    Source locationGBSeq[GBSeq_accession-version="AAT54156.1"]/GBSeq_feature-table; GBSeq_sequence
  • UniProtKB:Q81RQ6

    Source assertion: The reviewed carrier entry assigns aryl-carrier function and phosphopantetheinyl activation of Ser-40 with ECO:0000269.

    Source location/comments/0/texts; /comments/2/texts
  • PMID:17346033

    Source assertion: The abstract identifies AsbD as the carrier receiving the AsbC-activated aromatic unit.

    Source locationresultList/result[id="17346033"]/abstractText
Carrier phosphopantetheinylation
RHEA:48405		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

Aryl-carrier loading
RHEA:62477
  • RHEA:48405

    Source assertion: RHEA:48405 assigns RHEA-COMP:15903 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/48405 rh:products http://rdf.rhea-db.org/48404_R; http://rdf.rhea-db.org/48404_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_48404_compound_15903; rh:compound http://rdf.rhea-db.org/Compound_15903; rh:name and rh:reactivePart
  • RHEA:62477

    Source assertion: RHEA:62477 assigns RHEA-COMP:15903 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:substrates http://rdf.rhea-db.org/62476_L; http://rdf.rhea-db.org/62476_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_15903; rh:compound http://rdf.rhea-db.org/Compound_15903; rh:name and rh:reactivePart
3-dehydroshikimate dehydration
RHEA:24849		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.

3,4-dihydroxybenzoate adenylation
RHEA:62469
  • RHEA:24849

    Source assertion: RHEA:24849 assigns CHEBI:36241 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/24849 rh:products http://rdf.rhea-db.org/24848_R; http://rdf.rhea-db.org/24848_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_24848_compound_3099; rh:compound http://rdf.rhea-db.org/Compound_3099; rh:chebi
  • RHEA:62469

    Source assertion: RHEA:62469 assigns CHEBI:36241 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62469 rh:substrates http://rdf.rhea-db.org/62468_L; http://rdf.rhea-db.org/62468_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62468_compound_3099; rh:compound http://rdf.rhea-db.org/Compound_3099; rh:chebi
3,4-dihydroxybenzoate adenylation
RHEA:62469		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.

Aryl-carrier loading
RHEA:62477
  • RHEA:62469

    Source assertion: RHEA:62469 assigns CHEBI:144942 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62469 rh:products http://rdf.rhea-db.org/62468_R; http://rdf.rhea-db.org/62468_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62468_compound_16102; rh:compound http://rdf.rhea-db.org/Compound_16102; rh:chebi
  • RHEA:62477

    Source assertion: RHEA:62477 assigns CHEBI:144942 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:substrates http://rdf.rhea-db.org/62476_L; http://rdf.rhea-db.org/62476_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_16102; rh:compound http://rdf.rhea-db.org/Compound_16102; rh:chebi
Initial citrate-spermidine ligation
RHEA:63809		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.

Second ligation before aromatic transfer
RHEA:63813
  • RHEA:63809

    Source assertion: RHEA:63809 assigns CHEBI:149586 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63809 rh:products http://rdf.rhea-db.org/63808_R; http://rdf.rhea-db.org/63808_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63808_compound_16439; rh:compound http://rdf.rhea-db.org/Compound_16439; rh:chebi
  • RHEA:63813

    Source assertion: RHEA:63813 assigns CHEBI:149586 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63813 rh:substrates http://rdf.rhea-db.org/63812_L; http://rdf.rhea-db.org/63812_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63812_compound_16439; rh:compound http://rdf.rhea-db.org/Compound_16439; rh:chebi
Initial citrate-spermidine ligation
RHEA:63809		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.

Aromatic transfer before second ligation
RHEA:64029
  • RHEA:63809

    Source assertion: RHEA:63809 assigns CHEBI:149586 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63809 rh:products http://rdf.rhea-db.org/63808_R; http://rdf.rhea-db.org/63808_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63808_compound_16439; rh:compound http://rdf.rhea-db.org/Compound_16439; rh:chebi
  • RHEA:64029

    Source assertion: RHEA:64029 assigns CHEBI:149586 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64029 rh:substrates http://rdf.rhea-db.org/64028_L; http://rdf.rhea-db.org/64028_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64028_compound_16439; rh:compound http://rdf.rhea-db.org/Compound_16439; rh:chebi
Aromatic transfer before second ligation
RHEA:64029		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.

Second ligation after aromatic transfer
RHEA:63817
  • RHEA:64029

    Source assertion: RHEA:64029 assigns CHEBI:149593 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64029 rh:products http://rdf.rhea-db.org/64028_R; http://rdf.rhea-db.org/64028_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64028_compound_16442; rh:compound http://rdf.rhea-db.org/Compound_16442; rh:chebi
  • RHEA:63817

    Source assertion: RHEA:63817 assigns CHEBI:149593 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63817 rh:substrates http://rdf.rhea-db.org/63816_L; http://rdf.rhea-db.org/63816_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63816_compound_16442; rh:compound http://rdf.rhea-db.org/Compound_16442; rh:chebi
Second ligation before aromatic transfer
RHEA:63813		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.

Aromatic transfer on citryl-bis(spermidine)
RHEA:64025
  • RHEA:63813

    Source assertion: RHEA:63813 assigns CHEBI:149592 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63813 rh:products http://rdf.rhea-db.org/63812_R; http://rdf.rhea-db.org/63812_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63812_compound_16440; rh:compound http://rdf.rhea-db.org/Compound_16440; rh:chebi
  • RHEA:64025

    Source assertion: RHEA:64025 assigns CHEBI:149592 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64025 rh:substrates http://rdf.rhea-db.org/64024_L; http://rdf.rhea-db.org/64024_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64024_compound_16440; rh:compound http://rdf.rhea-db.org/Compound_16440; rh:chebi
Second ligation after aromatic transfer
RHEA:63817		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.

Terminal petrobactin aryl transfer
MetaCyc:RXN-10679
  • RHEA:63817

    Source assertion: RHEA:63817 assigns CHEBI:149594 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/63817 rh:products http://rdf.rhea-db.org/63816_R; http://rdf.rhea-db.org/63816_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_63816_compound_16441; rh:compound http://rdf.rhea-db.org/Compound_16441; rh:chebi
  • MetaCyc:RXN-10679

    Source assertion: The native terminal equation places CPD-11481 on its left side with unit coefficient and physiological left-to-right direction.

    Source locationReaction[@frameid="RXN-10679"]/left/*[@frameid="CPD-11481"]; reaction-direction
Aromatic transfer on citryl-bis(spermidine)
RHEA:64025		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order.

Terminal petrobactin aryl transfer
MetaCyc:RXN-10679
  • RHEA:64025

    Source assertion: RHEA:64025 assigns CHEBI:149594 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64025 rh:products http://rdf.rhea-db.org/64024_R; http://rdf.rhea-db.org/64024_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64024_compound_16441; rh:compound http://rdf.rhea-db.org/Compound_16441; rh:chebi
  • MetaCyc:RXN-10679

    Source assertion: The native terminal equation places CPD-11481 on its left side with unit coefficient and physiological left-to-right direction.

    Source locationReaction[@frameid="RXN-10679"]/left/*[@frameid="CPD-11481"]; reaction-direction
Aryl-carrier loading
RHEA:62477		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

Aromatic transfer before second ligation
RHEA:64029
  • RHEA:62477

    Source assertion: RHEA:62477 assigns RHEA-COMP:15941 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:products http://rdf.rhea-db.org/62476_R; http://rdf.rhea-db.org/62476_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_15941; rh:compound http://rdf.rhea-db.org/Compound_15941; rh:name and rh:reactivePart
  • RHEA:64029

    Source assertion: RHEA:64029 assigns RHEA-COMP:15941 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64029 rh:substrates http://rdf.rhea-db.org/64028_L; http://rdf.rhea-db.org/64028_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64028_compound_15941; rh:compound http://rdf.rhea-db.org/Compound_15941; rh:name and rh:reactivePart
Aryl-carrier loading
RHEA:62477		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

Aromatic transfer on citryl-bis(spermidine)
RHEA:64025
  • RHEA:62477

    Source assertion: RHEA:62477 assigns RHEA-COMP:15941 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:products http://rdf.rhea-db.org/62476_R; http://rdf.rhea-db.org/62476_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_15941; rh:compound http://rdf.rhea-db.org/Compound_15941; rh:name and rh:reactivePart
  • RHEA:64025

    Source assertion: RHEA:64025 assigns RHEA-COMP:15941 to its substrates with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/64025 rh:substrates http://rdf.rhea-db.org/64024_L; http://rdf.rhea-db.org/64024_L http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_64024_compound_15941; rh:compound http://rdf.rhea-db.org/Compound_15941; rh:name and rh:reactivePart
Aryl-carrier loading
RHEA:62477		provides_input_for

The independently sourced sides identify the shared intermediate. These convergent branches are supported in vitro; the graph does not choose a unique intracellular flux order. This is a whole generic aryl-carrier state, not free phosphopantetheine or an exact AsbD sequence. Chemical class coefficients do not specify enzyme assembly stoichiometry.

Terminal petrobactin aryl transfer
MetaCyc:RXN-10679
  • RHEA:62477

    Source assertion: RHEA:62477 assigns RHEA-COMP:15941 to its products with coefficient 1.

    Source locationhttp://rdf.rhea-db.org/62477 rh:products http://rdf.rhea-db.org/62476_R; http://rdf.rhea-db.org/62476_R http://rdf.rhea-db.org/contains1 http://rdf.rhea-db.org/Participant_62476_compound_15941; rh:compound http://rdf.rhea-db.org/Compound_15941; rh:name and rh:reactivePart
  • MetaCyc:RXN-10679

    Source assertion: The native terminal equation places 34-DHB-AsbD-Proteins on its left side with unit coefficient and physiological left-to-right direction.

    Source locationReaction[@frameid="RXN-10679"]/left/*[@frameid="34-DHB-AsbD-Proteins"]; reaction-direction

References

Curation history