molybdenum cofactor biosynthesis

MetaCyc:PWY-6823

The complete eight-reaction native route is represented for the Escherichia coli K-12 protein core: MoaA cyclizes GTP, MoaC forms cPMP, sulfur-carrier activation and recycling supply both sulfurs of molybdopterin, and Mog/MoeA adenylation and molybdate insertion yield Mo-molybdopterin. The sulfur-transfer net reaction retains five whole protein carrier states and a generic reductant; it does not specify every elementary relay. YnjE enhances reconstitution without a claim of universal indispensability. MoaA cyclase/cluster assignments retain their HAMAP inference, while direct MoaC experiments distinguish the E. coli enzyme from Staphylococcus aureus MoaA used in coupled assays. Native assemblies concern MG1655 within K-12; individual kinetic and genetic experiments have narrower strain and assay scopes. Anaerobic nitrate-respiration complementation and oxygen-sensitive radical-SAM preparations do not imply exclusive anaerobic pathway operation. The endpoint is unmodified Mo-MPT, before MCD or bis-MGD synthesis and cofactor insertion into target enzymes. The initial PathBank map also includes a separate folate branch and MocA cytidylation; those are outside this exact native boundary. Alternative sulfur-donor compensation, tungsten substitution, and high-molybdate bypass conditions do not establish uniform wild-type flux.

Pathway type

cofactor biosynthesis

Organism scope

Escherichia coli K-12 NCBITaxon:83333

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Pathway network

52 nodes · 73 asserted directed edges

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Escherichia coli K-12 `NCBITaxon:83333`	taxon
GTP 3',8-cyclase `UniProtKB:P30745`	protein
Cyclic pyranopterin monophosphate synthase `UniProtKB:P0A738`	protein
Molybdopterin synthase sulfur carrier subunit `UniProtKB:P30748`	protein
Molybdopterin synthase catalytic subunit `UniProtKB:P30749`	protein
Molybdopterin molybdenumtransferase `UniProtKB:P12281`	protein
Molybdopterin-synthase adenylyltransferase `UniProtKB:P12282`	protein
Molybdopterin adenylyltransferase `UniProtKB:P0AF03`	protein
Cysteine desulfurase IscS `UniProtKB:P0A6B7`	protein
Thiosulfate sulfurtransferase YnjE `UniProtKB:P78067`	protein
cyclic pyranopterin monophosphate synthase `MetaCyc:CPLX-8331`	complex
molybdopterin synthase `MetaCyc:CPLX0-2502`	complex
molybdopterin-synthase adenylyltransferase `MetaCyc:CPLX0-8164`	complex
molybdopterin adenylyltransferase `MetaCyc:CPLX0-8163`	complex
molybdopterin molybdotransferase `MetaCyc:CPLX0-8045`	complex
cysteine desulfurase `MetaCyc:CPLX0-248`	complex
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate(4-) `CHEBI:131766`	small molecule
hydrogen acceptor `CHEBI:13193`	small molecule
water `CHEBI:15377`	small molecule
hydron `CHEBI:15378`	small molecule
5'-deoxyadenosine `CHEBI:17319`	small molecule
hydrogen donor `CHEBI:17499`	small molecule
magnesium(2+) `CHEBI:18420`	cofactor
zinc(2+) `CHEBI:29105`	cofactor
ATP(4-) `CHEBI:30616`	small molecule
diphosphate(3-) `CHEBI:33019`	small molecule
L-cysteine zwitterion `CHEBI:35235`	small molecule
molybdate `CHEBI:36264`	small molecule
GTP(4-) `CHEBI:37565`	small molecule
adenosine 5'-monophosphate(2-) `CHEBI:456215`	small molecule
tetra-mu3-sulfido-tetrairon `CHEBI:49883`	cofactor
L-methionine zwitterion `CHEBI:57844`	small molecule
L-alanine zwitterion `CHEBI:57972`	small molecule
molybdopterin(3-) `CHEBI:58698`	small molecule
precursor Z(1-) `CHEBI:59648`	small molecule
pyridoxal 5'-phosphate(2-) `CHEBI:597326`	cofactor
S-adenosyl-L-methionine zwitterion `CHEBI:59789`	small molecule
molybdopterin adenine dinucleotide(3-) `CHEBI:62727`	small molecule
Mo(VI)O2(OH)-molybdopterin cofactor(4-) `CHEBI:71302`	small molecule
S-sulfanyl-L-cysteinyl-[cysteine desulfurase] `RHEA-COMP:12157`	protein
L-cysteinyl-[cysteine desulfurase] `RHEA-COMP:12158`	protein
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP `RHEA-COMP:12159`	protein
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly `RHEA-COMP:12202`	protein
[molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate `RHEA-COMP:19907`	protein
GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	molecular activity	left to right
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate => cyclic pyranopterin phosphate + diphosphate `RHEA:49581`	molecular activity	left to right
L-cysteinyl-[cysteine desulfurase] + L-cysteine => S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + L-alanine `RHEA:17458`	molecular activity	left to right
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) => [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate `RHEA:43617`	molecular activity	left to right
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	molecular activity	left to right
2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O => molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+) `RHEA:26334`	molecular activity	left to right
molybdopterin + ATP + H(+) => adenylyl-molybdopterin + diphosphate `RHEA:31332`	molecular activity	left to right
adenylyl-molybdopterin + molybdate => Mo-molybdopterin + AMP + H(+) `RHEA:35048`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
L-cysteinyl-[cysteine desulfurase] `RHEA-COMP:12158`	consumes Coefficient 1 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	L-cysteinyl-[cysteine desulfurase] + L-cysteine => S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + L-alanine `RHEA:17458`	RHEA:17457 Source assertion: RHEA:17458 substrates contain RHEA-COMP:12158 with coefficient 1. Source location `http://rdf.rhea-db.org/17458 rh:substrates; http://rdf.rhea-db.org/17457_L rh:contains1 http://rdf.rhea-db.org/Participant_17457_compound_12158; rh:compound http://rdf.rhea-db.org/Compound_12158`
L-cysteine zwitterion `CHEBI:35235`	consumes Coefficient 1 in the selected biosynthetic direction.	L-cysteinyl-[cysteine desulfurase] + L-cysteine => S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + L-alanine `RHEA:17458`	RHEA:17457 Source assertion: RHEA:17458 substrates contain CHEBI:35235 with coefficient 1. Source location `http://rdf.rhea-db.org/17458 rh:substrates; http://rdf.rhea-db.org/17457_L rh:contains1 http://rdf.rhea-db.org/Participant_17457_compound_2467; rh:compound http://rdf.rhea-db.org/Compound_2467`
L-cysteinyl-[cysteine desulfurase] + L-cysteine => S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + L-alanine `RHEA:17458`	produces Coefficient 1 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	S-sulfanyl-L-cysteinyl-[cysteine desulfurase] `RHEA-COMP:12157`	RHEA:17457 Source assertion: RHEA:17458 products contain RHEA-COMP:12157 with coefficient 1. Source location `http://rdf.rhea-db.org/17458 rh:products; http://rdf.rhea-db.org/17457_R rh:contains1 http://rdf.rhea-db.org/Participant_17457_compound_12157; rh:compound http://rdf.rhea-db.org/Compound_12157`
L-cysteinyl-[cysteine desulfurase] + L-cysteine => S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + L-alanine `RHEA:17458`	produces Coefficient 1 in the selected biosynthetic direction.	L-alanine zwitterion `CHEBI:57972`	RHEA:17457 Source assertion: RHEA:17458 products contain CHEBI:57972 with coefficient 1. Source location `http://rdf.rhea-db.org/17458 rh:products; http://rdf.rhea-db.org/17457_R rh:contains1 http://rdf.rhea-db.org/Participant_17457_compound_3361; rh:compound http://rdf.rhea-db.org/Compound_3361`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate `RHEA-COMP:19907`	consumes Coefficient 2 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O => molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+) `RHEA:26334`	RHEA:26333 Source assertion: RHEA:26334 substrates contain RHEA-COMP:19907 with coefficient 2. Source location `http://rdf.rhea-db.org/26334 rh:substrates; http://rdf.rhea-db.org/26333_L rh:contains2 http://rdf.rhea-db.org/Participant_26333_compound_19907; rh:compound http://rdf.rhea-db.org/Compound_19907`
precursor Z(1-) `CHEBI:59648`	consumes Coefficient 1 in the selected biosynthetic direction.	2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O => molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+) `RHEA:26334`	RHEA:26333 Source assertion: RHEA:26334 substrates contain CHEBI:59648 with coefficient 1. Source location `http://rdf.rhea-db.org/26334 rh:substrates; http://rdf.rhea-db.org/26333_L rh:contains1 http://rdf.rhea-db.org/Participant_26333_compound_5980; rh:compound http://rdf.rhea-db.org/Compound_5980`
water `CHEBI:15377`	consumes Coefficient 1 in the selected biosynthetic direction.	2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O => molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+) `RHEA:26334`	RHEA:26333 Source assertion: RHEA:26334 substrates contain CHEBI:15377 with coefficient 1. Source location `http://rdf.rhea-db.org/26334 rh:substrates; http://rdf.rhea-db.org/26333_L rh:contains1 http://rdf.rhea-db.org/Participant_26333_compound_1283; rh:compound http://rdf.rhea-db.org/Compound_1283`
2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O => molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+) `RHEA:26334`	produces Coefficient 1 in the selected biosynthetic direction.	molybdopterin(3-) `CHEBI:58698`	RHEA:26333 Source assertion: RHEA:26334 products contain CHEBI:58698 with coefficient 1. Source location `http://rdf.rhea-db.org/26334 rh:products; http://rdf.rhea-db.org/26333_R rh:contains1 http://rdf.rhea-db.org/Participant_26333_compound_3616; rh:compound http://rdf.rhea-db.org/Compound_3616`
2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O => molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+) `RHEA:26334`	produces Coefficient 2 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly `RHEA-COMP:12202`	RHEA:26333 Source assertion: RHEA:26334 products contain RHEA-COMP:12202 with coefficient 2. Source location `http://rdf.rhea-db.org/26334 rh:products; http://rdf.rhea-db.org/26333_R rh:contains2 http://rdf.rhea-db.org/Participant_26333_compound_12202; rh:compound http://rdf.rhea-db.org/Compound_12202`
2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O => molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+) `RHEA:26334`	produces Coefficient 2 in the selected biosynthetic direction.	hydron `CHEBI:15378`	RHEA:26333 Source assertion: RHEA:26334 products contain CHEBI:15378 with coefficient 2. Source location `http://rdf.rhea-db.org/26334 rh:products; http://rdf.rhea-db.org/26333_R rh:contains2 http://rdf.rhea-db.org/Participant_26333_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249`
molybdopterin(3-) `CHEBI:58698`	consumes Coefficient 1 in the selected biosynthetic direction.	molybdopterin + ATP + H(+) => adenylyl-molybdopterin + diphosphate `RHEA:31332`	RHEA:31331 Source assertion: RHEA:31332 substrates contain CHEBI:58698 with coefficient 1. Source location `http://rdf.rhea-db.org/31332 rh:substrates; http://rdf.rhea-db.org/31331_L rh:contains1 http://rdf.rhea-db.org/Participant_31331_compound_3616; rh:compound http://rdf.rhea-db.org/Compound_3616`
ATP(4-) `CHEBI:30616`	consumes Coefficient 1 in the selected biosynthetic direction.	molybdopterin + ATP + H(+) => adenylyl-molybdopterin + diphosphate `RHEA:31332`	RHEA:31331 Source assertion: RHEA:31332 substrates contain CHEBI:30616 with coefficient 1. Source location `http://rdf.rhea-db.org/31332 rh:substrates; http://rdf.rhea-db.org/31331_L rh:contains1 http://rdf.rhea-db.org/Participant_31331_compound_6372; rh:compound http://rdf.rhea-db.org/Compound_6372`
hydron `CHEBI:15378`	consumes Coefficient 1 in the selected biosynthetic direction.	molybdopterin + ATP + H(+) => adenylyl-molybdopterin + diphosphate `RHEA:31332`	RHEA:31331 Source assertion: RHEA:31332 substrates contain CHEBI:15378 with coefficient 1. Source location `http://rdf.rhea-db.org/31332 rh:substrates; http://rdf.rhea-db.org/31331_L rh:contains1 http://rdf.rhea-db.org/Participant_31331_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249`
molybdopterin + ATP + H(+) => adenylyl-molybdopterin + diphosphate `RHEA:31332`	produces Coefficient 1 in the selected biosynthetic direction.	molybdopterin adenine dinucleotide(3-) `CHEBI:62727`	RHEA:31331 Source assertion: RHEA:31332 products contain CHEBI:62727 with coefficient 1. Source location `http://rdf.rhea-db.org/31332 rh:products; http://rdf.rhea-db.org/31331_R rh:contains1 http://rdf.rhea-db.org/Participant_31331_compound_6867; rh:compound http://rdf.rhea-db.org/Compound_6867`
molybdopterin + ATP + H(+) => adenylyl-molybdopterin + diphosphate `RHEA:31332`	produces Coefficient 1 in the selected biosynthetic direction.	diphosphate(3-) `CHEBI:33019`	RHEA:31331 Source assertion: RHEA:31332 products contain CHEBI:33019 with coefficient 1. Source location `http://rdf.rhea-db.org/31332 rh:products; http://rdf.rhea-db.org/31331_R rh:contains1 http://rdf.rhea-db.org/Participant_31331_compound_2106; rh:compound http://rdf.rhea-db.org/Compound_2106`
molybdopterin adenine dinucleotide(3-) `CHEBI:62727`	consumes Coefficient 1 in the selected biosynthetic direction.	adenylyl-molybdopterin + molybdate => Mo-molybdopterin + AMP + H(+) `RHEA:35048`	RHEA:35047 Source assertion: RHEA:35048 substrates contain CHEBI:62727 with coefficient 1. Source location `http://rdf.rhea-db.org/35048 rh:substrates; http://rdf.rhea-db.org/35047_L rh:contains1 http://rdf.rhea-db.org/Participant_35047_compound_6867; rh:compound http://rdf.rhea-db.org/Compound_6867`
molybdate `CHEBI:36264`	consumes Coefficient 1 in the selected biosynthetic direction.	adenylyl-molybdopterin + molybdate => Mo-molybdopterin + AMP + H(+) `RHEA:35048`	RHEA:35047 Source assertion: RHEA:35048 substrates contain CHEBI:36264 with coefficient 1. Source location `http://rdf.rhea-db.org/35048 rh:substrates; http://rdf.rhea-db.org/35047_L rh:contains1 http://rdf.rhea-db.org/Participant_35047_compound_5442; rh:compound http://rdf.rhea-db.org/Compound_5442`
adenylyl-molybdopterin + molybdate => Mo-molybdopterin + AMP + H(+) `RHEA:35048`	produces Coefficient 1 in the selected biosynthetic direction.	Mo(VI)O2(OH)-molybdopterin cofactor(4-) `CHEBI:71302`	RHEA:35047 Source assertion: RHEA:35048 products contain CHEBI:71302 with coefficient 1. Source location `http://rdf.rhea-db.org/35048 rh:products; http://rdf.rhea-db.org/35047_R rh:contains1 http://rdf.rhea-db.org/Participant_35047_compound_7664; rh:compound http://rdf.rhea-db.org/Compound_7664`
adenylyl-molybdopterin + molybdate => Mo-molybdopterin + AMP + H(+) `RHEA:35048`	produces Coefficient 1 in the selected biosynthetic direction.	adenosine 5'-monophosphate(2-) `CHEBI:456215`	RHEA:35047 Source assertion: RHEA:35048 products contain CHEBI:456215 with coefficient 1. Source location `http://rdf.rhea-db.org/35048 rh:products; http://rdf.rhea-db.org/35047_R rh:contains1 http://rdf.rhea-db.org/Participant_35047_compound_2590; rh:compound http://rdf.rhea-db.org/Compound_2590`
adenylyl-molybdopterin + molybdate => Mo-molybdopterin + AMP + H(+) `RHEA:35048`	produces Coefficient 1 in the selected biosynthetic direction.	hydron `CHEBI:15378`	RHEA:35047 Source assertion: RHEA:35048 products contain CHEBI:15378 with coefficient 1. Source location `http://rdf.rhea-db.org/35048 rh:products; http://rdf.rhea-db.org/35047_R rh:contains1 http://rdf.rhea-db.org/Participant_35047_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly `RHEA-COMP:12202`	consumes Coefficient 1 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) => [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate `RHEA:43617`	RHEA:43616 Source assertion: RHEA:43617 substrates contain RHEA-COMP:12202 with coefficient 1. Source location `http://rdf.rhea-db.org/43617 rh:substrates; http://rdf.rhea-db.org/43616_L rh:contains1 http://rdf.rhea-db.org/Participant_43616_compound_12202; rh:compound http://rdf.rhea-db.org/Compound_12202`
ATP(4-) `CHEBI:30616`	consumes Coefficient 1 in the selected biosynthetic direction.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) => [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate `RHEA:43617`	RHEA:43616 Source assertion: RHEA:43617 substrates contain CHEBI:30616 with coefficient 1. Source location `http://rdf.rhea-db.org/43617 rh:substrates; http://rdf.rhea-db.org/43616_L rh:contains1 http://rdf.rhea-db.org/Participant_43616_compound_6372; rh:compound http://rdf.rhea-db.org/Compound_6372`
hydron `CHEBI:15378`	consumes Coefficient 1 in the selected biosynthetic direction.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) => [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate `RHEA:43617`	RHEA:43616 Source assertion: RHEA:43617 substrates contain CHEBI:15378 with coefficient 1. Source location `http://rdf.rhea-db.org/43617 rh:substrates; http://rdf.rhea-db.org/43616_L rh:contains1 http://rdf.rhea-db.org/Participant_43616_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) => [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate `RHEA:43617`	produces Coefficient 1 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP `RHEA-COMP:12159`	RHEA:43616 Source assertion: RHEA:43617 products contain RHEA-COMP:12159 with coefficient 1. Source location `http://rdf.rhea-db.org/43617 rh:products; http://rdf.rhea-db.org/43616_R rh:contains1 http://rdf.rhea-db.org/Participant_43616_compound_12159; rh:compound http://rdf.rhea-db.org/Compound_12159`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) => [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate `RHEA:43617`	produces Coefficient 1 in the selected biosynthetic direction.	diphosphate(3-) `CHEBI:33019`	RHEA:43616 Source assertion: RHEA:43617 products contain CHEBI:33019 with coefficient 1. Source location `http://rdf.rhea-db.org/43617 rh:products; http://rdf.rhea-db.org/43616_R rh:contains1 http://rdf.rhea-db.org/Participant_43616_compound_2106; rh:compound http://rdf.rhea-db.org/Compound_2106`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP `RHEA-COMP:12159`	consumes Coefficient 1 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	RHEA:48612 Source assertion: RHEA:48613 substrates contain RHEA-COMP:12159 with coefficient 1. Source location `http://rdf.rhea-db.org/48613 rh:substrates; http://rdf.rhea-db.org/48612_L rh:contains1 http://rdf.rhea-db.org/Participant_48612_compound_12159; rh:compound http://rdf.rhea-db.org/Compound_12159`
S-sulfanyl-L-cysteinyl-[cysteine desulfurase] `RHEA-COMP:12157`	consumes Coefficient 1 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	RHEA:48612 Source assertion: RHEA:48613 substrates contain RHEA-COMP:12157 with coefficient 1. Source location `http://rdf.rhea-db.org/48613 rh:substrates; http://rdf.rhea-db.org/48612_L rh:contains1 http://rdf.rhea-db.org/Participant_48612_compound_12157; rh:compound http://rdf.rhea-db.org/Compound_12157`
hydrogen donor `CHEBI:17499`	consumes Coefficient 1 in the selected biosynthetic direction. The net redox donor/acceptor remains generic; these sources do not identify the physiological electron donor.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	RHEA:48612 Source assertion: RHEA:48613 substrates contain CHEBI:17499 with coefficient 1. Source location `http://rdf.rhea-db.org/48613 rh:substrates; http://rdf.rhea-db.org/48612_L rh:contains1 http://rdf.rhea-db.org/Participant_48612_compound_1304; rh:compound http://rdf.rhea-db.org/Compound_1304`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	produces Coefficient 1 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate `RHEA-COMP:19907`	RHEA:48612 Source assertion: RHEA:48613 products contain RHEA-COMP:19907 with coefficient 1. Source location `http://rdf.rhea-db.org/48613 rh:products; http://rdf.rhea-db.org/48612_R rh:contains1 http://rdf.rhea-db.org/Participant_48612_compound_19907; rh:compound http://rdf.rhea-db.org/Compound_19907`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	produces Coefficient 1 in the selected biosynthetic direction. This is the whole protein carrier state; its chemically modified residue is not a free metabolite.	L-cysteinyl-[cysteine desulfurase] `RHEA-COMP:12158`	RHEA:48612 Source assertion: RHEA:48613 products contain RHEA-COMP:12158 with coefficient 1. Source location `http://rdf.rhea-db.org/48613 rh:products; http://rdf.rhea-db.org/48612_R rh:contains1 http://rdf.rhea-db.org/Participant_48612_compound_12158; rh:compound http://rdf.rhea-db.org/Compound_12158`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	produces Coefficient 1 in the selected biosynthetic direction. The net redox donor/acceptor remains generic; these sources do not identify the physiological electron donor.	hydrogen acceptor `CHEBI:13193`	RHEA:48612 Source assertion: RHEA:48613 products contain CHEBI:13193 with coefficient 1. Source location `http://rdf.rhea-db.org/48613 rh:products; http://rdf.rhea-db.org/48612_R rh:contains1 http://rdf.rhea-db.org/Participant_48612_compound_3512; rh:compound http://rdf.rhea-db.org/Compound_3512`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	produces Coefficient 1 in the selected biosynthetic direction.	adenosine 5'-monophosphate(2-) `CHEBI:456215`	RHEA:48612 Source assertion: RHEA:48613 products contain CHEBI:456215 with coefficient 1. Source location `http://rdf.rhea-db.org/48613 rh:products; http://rdf.rhea-db.org/48612_R rh:contains1 http://rdf.rhea-db.org/Participant_48612_compound_2590; rh:compound http://rdf.rhea-db.org/Compound_2590`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	produces Coefficient 2 in the selected biosynthetic direction.	hydron `CHEBI:15378`	RHEA:48612 Source assertion: RHEA:48613 products contain CHEBI:15378 with coefficient 2. Source location `http://rdf.rhea-db.org/48613 rh:products; http://rdf.rhea-db.org/48612_R rh:contains2 http://rdf.rhea-db.org/Participant_48612_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249`
GTP(4-) `CHEBI:37565`	consumes Coefficient 1 in the selected biosynthetic direction.	GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	RHEA:49576 Source assertion: RHEA:49577 substrates contain CHEBI:37565 with coefficient 1. Source location `http://rdf.rhea-db.org/49577 rh:substrates; http://rdf.rhea-db.org/49576_L rh:contains1 http://rdf.rhea-db.org/Participant_49576_compound_6355; rh:compound http://rdf.rhea-db.org/Compound_6355`
hydrogen donor `CHEBI:17499`	consumes Coefficient 1 in the selected biosynthetic direction. The net redox donor/acceptor remains generic; these sources do not identify the physiological electron donor.	GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	RHEA:49576 Source assertion: RHEA:49577 substrates contain CHEBI:17499 with coefficient 1. Source location `http://rdf.rhea-db.org/49577 rh:substrates; http://rdf.rhea-db.org/49576_L rh:contains1 http://rdf.rhea-db.org/Participant_49576_compound_1304; rh:compound http://rdf.rhea-db.org/Compound_1304`
S-adenosyl-L-methionine zwitterion `CHEBI:59789`	consumes Coefficient 1 in the selected biosynthetic direction.	GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	RHEA:49576 Source assertion: RHEA:49577 substrates contain CHEBI:59789 with coefficient 1. Source location `http://rdf.rhea-db.org/49577 rh:substrates; http://rdf.rhea-db.org/49576_L rh:contains1 http://rdf.rhea-db.org/Participant_49576_compound_6127; rh:compound http://rdf.rhea-db.org/Compound_6127`
GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	produces Coefficient 1 in the selected biosynthetic direction.	(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate(4-) `CHEBI:131766`	RHEA:49576 Source assertion: RHEA:49577 products contain CHEBI:131766 with coefficient 1. Source location `http://rdf.rhea-db.org/49577 rh:products; http://rdf.rhea-db.org/49576_R rh:contains1 http://rdf.rhea-db.org/Participant_49576_compound_12441; rh:compound http://rdf.rhea-db.org/Compound_12441`
GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	produces Coefficient 1 in the selected biosynthetic direction.	5'-deoxyadenosine `CHEBI:17319`	RHEA:49576 Source assertion: RHEA:49577 products contain CHEBI:17319 with coefficient 1. Source location `http://rdf.rhea-db.org/49577 rh:products; http://rdf.rhea-db.org/49576_R rh:contains1 http://rdf.rhea-db.org/Participant_49576_compound_2028; rh:compound http://rdf.rhea-db.org/Compound_2028`
GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	produces Coefficient 1 in the selected biosynthetic direction.	L-methionine zwitterion `CHEBI:57844`	RHEA:49576 Source assertion: RHEA:49577 products contain CHEBI:57844 with coefficient 1. Source location `http://rdf.rhea-db.org/49577 rh:products; http://rdf.rhea-db.org/49576_R rh:contains1 http://rdf.rhea-db.org/Participant_49576_compound_2530; rh:compound http://rdf.rhea-db.org/Compound_2530`
GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	produces Coefficient 1 in the selected biosynthetic direction. The net redox donor/acceptor remains generic; these sources do not identify the physiological electron donor.	hydrogen acceptor `CHEBI:13193`	RHEA:49576 Source assertion: RHEA:49577 products contain CHEBI:13193 with coefficient 1. Source location `http://rdf.rhea-db.org/49577 rh:products; http://rdf.rhea-db.org/49576_R rh:contains1 http://rdf.rhea-db.org/Participant_49576_compound_3512; rh:compound http://rdf.rhea-db.org/Compound_3512`
GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	produces Coefficient 1 in the selected biosynthetic direction.	hydron `CHEBI:15378`	RHEA:49576 Source assertion: RHEA:49577 products contain CHEBI:15378 with coefficient 1. Source location `http://rdf.rhea-db.org/49577 rh:products; http://rdf.rhea-db.org/49576_R rh:contains1 http://rdf.rhea-db.org/Participant_49576_compound_3249; rh:compound http://rdf.rhea-db.org/Compound_3249`
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate(4-) `CHEBI:131766`	consumes Coefficient 1 in the selected biosynthetic direction.	(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate => cyclic pyranopterin phosphate + diphosphate `RHEA:49581`	RHEA:49580 Source assertion: RHEA:49581 substrates contain CHEBI:131766 with coefficient 1. Source location `http://rdf.rhea-db.org/49581 rh:substrates; http://rdf.rhea-db.org/49580_L rh:contains1 http://rdf.rhea-db.org/Participant_49580_compound_12441; rh:compound http://rdf.rhea-db.org/Compound_12441`
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate => cyclic pyranopterin phosphate + diphosphate `RHEA:49581`	produces Coefficient 1 in the selected biosynthetic direction.	precursor Z(1-) `CHEBI:59648`	RHEA:49580 Source assertion: RHEA:49581 products contain CHEBI:59648 with coefficient 1. Source location `http://rdf.rhea-db.org/49581 rh:products; http://rdf.rhea-db.org/49580_R rh:contains1 http://rdf.rhea-db.org/Participant_49580_compound_5980; rh:compound http://rdf.rhea-db.org/Compound_5980`
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate => cyclic pyranopterin phosphate + diphosphate `RHEA:49581`	produces Coefficient 1 in the selected biosynthetic direction.	diphosphate(3-) `CHEBI:33019`	RHEA:49580 Source assertion: RHEA:49581 products contain CHEBI:33019 with coefficient 1. Source location `http://rdf.rhea-db.org/49581 rh:products; http://rdf.rhea-db.org/49580_R rh:contains1 http://rdf.rhea-db.org/Participant_49580_compound_2106; rh:compound http://rdf.rhea-db.org/Compound_2106`
GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	part_of The native pathway reaction is represented in its biosynthetic direction using exact current Rhea chemistry. Native RXN-8340 has a stale RHEA:26378 cross-reference, which denotes the combined GTP-to-cPMP transformation. Its actual cyclic-GTP product and EC 4.1.99.22, together with current MoaA annotation, support the separate cyclase step here.	`MetaCyc:PWY-6823`	MetaCyc:PWY-6823 Source assertion: PWY-6823 includes RXN-8340 in its eight-reaction molybdenum-cofactor route. Source location `/ptools-xml/[@frameid='PWY-6823']/reaction-list/Reaction[@frameid="RXN-8340"]` RHEA:49576 Source assertion:* RHEA:49576 has directional reaction RHEA:49577, selected left-to-right for biosynthesis. Source location `http://rdf.rhea-db.org/49576 rh:directionalReaction http://rdf.rhea-db.org/49577; http://rdf.rhea-db.org/49577 rh:substrates/rh:products` MetaCyc:RXN-8340 Source assertion: RXN-8340 consumes GTP and produces CPD-19179; EC 4.1.99.22 describes GTP cyclization. Its RHEA:26378 cross-reference is not used as an identity equivalence. Source location `/ptools-xml/[@frameid='RXN-8340']/left; right; ec-number; dblink` MetaCyc:CPD-19179 Source assertion:* CPD-19179 names (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate; its CML gives C10H12N5O14P3 and charge -4. Source location `/ptools-xml/Compound[@frameid='CPD-19179']/common-name; cml/molecule/formula; cml/molecule/@formalCharge` UniProtKB:P30745 Source assertion: The reviewed MoaA annotation links EC 4.1.99.22 to RHEA:49576 with ECO:0000255 (HAMAP MF_01225). Source location `$.comments[2].reaction` CHEBI:131766 Source assertion: ChEBI:131766 explicitly cross-references metacyc.compound:CPD-19179 and identifies the (8S) cyclic GTP structure, formula C10H12N5O14P3, charge -4. Source location `ChEBI 255 Semantic SQL statements: subject CHEBI:131766; oio:hasDbXref = metacyc.compound:CPD-19179; rdfs:label; chemrof:generalized_empirical_formula; chemrof:charge`
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate => cyclic pyranopterin phosphate + diphosphate `RHEA:49581`	part_of The native pathway reaction is represented in its biosynthetic direction using exact current Rhea chemistry.	`MetaCyc:PWY-6823`	MetaCyc:PWY-6823 Source assertion: PWY-6823 includes RXN-17809 in its eight-reaction molybdenum-cofactor route. Source location `/ptools-xml/[@frameid='PWY-6823']/reaction-list/Reaction[@frameid="RXN-17809"]` RHEA:49580 Source assertion:* RHEA:49580 has directional reaction RHEA:49581, selected left-to-right for biosynthesis. Source location `http://rdf.rhea-db.org/49580 rh:directionalReaction http://rdf.rhea-db.org/49581; http://rdf.rhea-db.org/49581 rh:substrates/rh:products` MetaCyc:RXN-17809 Source assertion: RXN-17809 explicitly cross-references RHEA:49581; its native left-to-right reaction is selected. Source location `/ptools-xml/*[@frameid='RXN-17809']/reaction-direction; dblink[dblink-db="RHEA"]`
L-cysteinyl-[cysteine desulfurase] + L-cysteine => S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + L-alanine `RHEA:17458`	part_of The native pathway reaction is represented in its biosynthetic direction using exact current Rhea chemistry.	`MetaCyc:PWY-6823`	MetaCyc:PWY-6823 Source assertion: PWY-6823 includes RXN0-308 in its eight-reaction molybdenum-cofactor route. Source location `/ptools-xml/[@frameid='PWY-6823']/reaction-list/Reaction[@frameid="RXN0-308"]` RHEA:17457 Source assertion:* RHEA:17457 has directional reaction RHEA:17458, selected left-to-right for biosynthesis. Source location `http://rdf.rhea-db.org/17457 rh:directionalReaction http://rdf.rhea-db.org/17458; http://rdf.rhea-db.org/17458 rh:substrates/rh:products` MetaCyc:RXN0-308 Source assertion: RXN0-308 explicitly cross-references RHEA:17458; its native left-to-right reaction is selected. Source location `/ptools-xml/*[@frameid='RXN0-308']/reaction-direction; dblink[dblink-db="RHEA"]`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) => [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate `RHEA:43617`	part_of The native pathway reaction is represented in its biosynthetic direction using exact current Rhea chemistry.	`MetaCyc:PWY-6823`	MetaCyc:PWY-6823 Source assertion: PWY-6823 includes RXN-11361 in its eight-reaction molybdenum-cofactor route. Source location `/ptools-xml/[@frameid='PWY-6823']/reaction-list/Reaction[@frameid="RXN-11361"]` RHEA:43616 Source assertion:* RHEA:43616 has directional reaction RHEA:43617, selected left-to-right for biosynthesis. Source location `http://rdf.rhea-db.org/43616 rh:directionalReaction http://rdf.rhea-db.org/43617; http://rdf.rhea-db.org/43617 rh:substrates/rh:products` MetaCyc:RXN-11361 Source assertion: RXN-11361 explicitly cross-references RHEA:43617; its native left-to-right reaction is selected. Source location `/ptools-xml/*[@frameid='RXN-11361']/reaction-direction; dblink[dblink-db="RHEA"]`
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	part_of The native pathway reaction is represented in its biosynthetic direction using exact current Rhea chemistry.	`MetaCyc:PWY-6823`	MetaCyc:PWY-6823 Source assertion: PWY-6823 includes RXN-12473 in its eight-reaction molybdenum-cofactor route. Source location `/ptools-xml/[@frameid='PWY-6823']/reaction-list/Reaction[@frameid="RXN-12473"]` RHEA:48612 Source assertion:* RHEA:48612 has directional reaction RHEA:48613, selected left-to-right for biosynthesis. Source location `http://rdf.rhea-db.org/48612 rh:directionalReaction http://rdf.rhea-db.org/48613; http://rdf.rhea-db.org/48613 rh:substrates/rh:products` MetaCyc:RXN-12473 Source assertion: RXN-12473 explicitly cross-references RHEA:48613; its native left-to-right reaction is selected. Source location `/ptools-xml/*[@frameid='RXN-12473']/reaction-direction; dblink[dblink-db="RHEA"]`
2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O => molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+) `RHEA:26334`	part_of The native pathway reaction is represented in its biosynthetic direction using exact current Rhea chemistry. The older native equation releases four protons; current Rhea releases two for its defined carrier and ionic forms.	`MetaCyc:PWY-6823`	MetaCyc:PWY-6823 Source assertion: PWY-6823 includes RXN-8342 in its eight-reaction molybdenum-cofactor route. Source location `/ptools-xml/[@frameid='PWY-6823']/reaction-list/Reaction[@frameid="RXN-8342"]` RHEA:26333 Source assertion:* RHEA:26333 has directional reaction RHEA:26334, selected left-to-right for biosynthesis. Source location `http://rdf.rhea-db.org/26333 rh:directionalReaction http://rdf.rhea-db.org/26334; http://rdf.rhea-db.org/26334 rh:substrates/rh:products` MetaCyc:RXN-8342 Source assertion: RXN-8342 explicitly cross-references RHEA:26334; its native left-to-right reaction is selected. Source location `/ptools-xml/*[@frameid='RXN-8342']/reaction-direction; dblink[dblink-db="RHEA"]`
molybdopterin + ATP + H(+) => adenylyl-molybdopterin + diphosphate `RHEA:31332`	part_of The native pathway reaction is represented in its biosynthetic direction using exact current Rhea chemistry.	`MetaCyc:PWY-6823`	MetaCyc:PWY-6823 Source assertion: PWY-6823 includes RXN-8344 in its eight-reaction molybdenum-cofactor route. Source location `/ptools-xml/[@frameid='PWY-6823']/reaction-list/Reaction[@frameid="RXN-8344"]` RHEA:31331 Source assertion:* RHEA:31331 has directional reaction RHEA:31332, selected left-to-right for biosynthesis. Source location `http://rdf.rhea-db.org/31331 rh:directionalReaction http://rdf.rhea-db.org/31332; http://rdf.rhea-db.org/31332 rh:substrates/rh:products` MetaCyc:RXN-8344 Source assertion: RXN-8344 explicitly cross-references RHEA:31332; its native left-to-right reaction is selected. Source location `/ptools-xml/*[@frameid='RXN-8344']/reaction-direction; dblink[dblink-db="RHEA"]`
adenylyl-molybdopterin + molybdate => Mo-molybdopterin + AMP + H(+) `RHEA:35048`	part_of The native pathway reaction is represented in its biosynthetic direction using exact current Rhea chemistry. The older native product is MoO2-MPT, whereas current Rhea specifies Mo(VI)O2(OH)-MPT; their water/proton accounting is different and is not treated as identical chemistry.	`MetaCyc:PWY-6823`	MetaCyc:PWY-6823 Source assertion: PWY-6823 includes RXN-8348 in its eight-reaction molybdenum-cofactor route. Source location `/ptools-xml/[@frameid='PWY-6823']/reaction-list/Reaction[@frameid="RXN-8348"]` RHEA:35047 Source assertion:* RHEA:35047 has directional reaction RHEA:35048, selected left-to-right for biosynthesis. Source location `http://rdf.rhea-db.org/35047 rh:directionalReaction http://rdf.rhea-db.org/35048; http://rdf.rhea-db.org/35048 rh:substrates/rh:products` MetaCyc:RXN-8348 Source assertion: RXN-8348 explicitly cross-references RHEA:35048; its native left-to-right reaction is selected. Source location `/ptools-xml/[@frameid='RXN-8348']/reaction-direction; dblink[dblink-db="RHEA"]` MetaCyc:CPD-8123 Source assertion:* CPD-8123 links CHEBI:71302 but its native CML represents MoO2-MPT with formula C10H10MoN5O8PS2 and charge -2. The graph uses the current Rhea chemical form instead. Source location `/ptools-xml/*[@frameid='CPD-8123']/cml/molecule/@formalCharge; cml/molecule/formula; dblink`
GTP 3',8-cyclase `UniProtKB:P30745`	enables The K-12 MoaA cyclase assignment is inferred by HAMAP. The inspected purified-enzyme cyclase work used Staphylococcus aureus MoaA; E. coli expression hosts do not make those experiments direct evidence for the K-12 enzyme. Current monomer/homodimer annotation does not establish an isolated monomer requirement.	GTP + AH2 + S-adenosyl-L-methionine => (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+) `RHEA:49577`	UniProtKB:P30745 Source assertion: P30745 is annotated for RHEA:49576 by ECO:0000255, HAMAP MF_01225. Source location `$.comments[2].reaction` MetaCyc:EG11595-MONOMER Source assertion: The E. coli MoaA frame links UniProt P30745 and catalyzes ENZRXN-19108. Source location `/ptools-xml/[@frameid='EG11595-MONOMER']/dblink; catalyzes` MetaCyc:ENZRXN-19108 Source assertion:* ENZRXN-19108 assigns EG11595-MONOMER to RXN-8340. Source location `/ptools-xml/*[@frameid='ENZRXN-19108']/enzyme; reaction`
cyclic pyranopterin monophosphate synthase `MetaCyc:CPLX-8331`	catalyzes MoaC converts the cyclized GTP intermediate into cPMP. Direct E. coli kinetic, structural and complementation experiments support this distinct second step; anaerobic nitrate respiration was a complementation readout, not a claim that all pathway steps require an anaerobic cell.	(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate => cyclic pyranopterin phosphate + diphosphate `RHEA:49581`	MetaCyc:CPLX-8331 Source assertion: The MoaC complex catalyzes RXN-17809. Source location `/ptools-xml/[@frameid='CPLX-8331']/catalyzes/Enzymatic-Reaction/reaction` UniProtKB:P0A738 Source assertion:* P0A738 is annotated for RHEA:49580 with ECO:0000269 (PMID:25941396), alongside HAMAP ECO:0000255. Source location `$.comments[1].reaction` PMID:25941396 Source assertion: E. coli MoaC variants were purified and tested with isolated cyclic GTP; wild-type MoaC rescued E. coli delta-moaC under anaerobic nitrate respiration. Source location `Results; Figure 1E-G; Materials and Methods`
cysteine desulfurase `MetaCyc:CPLX0-248`	catalyzes IscS mobilizes cysteine sulfur through its own cysteinyl persulfide. This explicit carrier-state reaction is supported by the native reaction; the broader UniProt sulfur-acceptor reaction is not substituted for it.	L-cysteinyl-[cysteine desulfurase] + L-cysteine => S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + L-alanine `RHEA:17458`	MetaCyc:CPLX0-248 Source assertion: The IscS complex catalyzes RXN0-308. Source location `/ptools-xml/[@frameid='CPLX0-248']/catalyzes/Enzymatic-Reaction/reaction` UniProtKB:P0A6B7 Source assertion:* The reviewed IscS function removes sulfur from cysteine to form alanine and delivers sulfur to multiple partners; multiple ECO:0000269 citations support this role. Source location `$.comments[0].texts` MetaCyc:RXN0-308 Source assertion: RXN0-308 links the cysteine-desulfurase carrier-state reaction to RHEA:17458. Source location `/ptools-xml/*[@frameid='RXN0-308']/left; right; dblink[dblink-db="RHEA"]`
molybdopterin-synthase adenylyltransferase `MetaCyc:CPLX0-8164`	catalyzes The MoeB homodimer adenylates the MoaD C terminus. A transient MoeB2-MoaD2 complex forms during activation; this does not assert catalysis without the MoaD substrate or a MoeB-bound sulfur intermediate.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) => [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate `RHEA:43617`	MetaCyc:CPLX0-8164 Source assertion: The MoeB complex catalyzes RXN-11361. Source location `/ptools-xml/[@frameid='CPLX0-8164']/catalyzes/Enzymatic-Reaction/reaction` UniProtKB:P12282 Source assertion:* P12282 is annotated for MoaD adenylation, RHEA:43616, with ECO:0000269 (PMID:11463785). Source location `$.comments[1].reaction` UniProtKB:P12282 Source assertion: MoeB is a homodimer and forms a stable two-MoeB/two-MoaD complex during MoaD adenylation; ECO:0000269 (PMID:11463785). Source location `$.comments[4].texts`
Cysteine desulfurase IscS `UniProtKB:P0A6B7`	enables IscS provides sulfur for the net thiocarboxylation of adenylated MoaD in the reconstituted MoeB/MoaD system. The graph retains the generic reductant and does not assert that isolated IscS carries out every elementary step. Alternative cysteine desulfurases can donate sulfur in vitro.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	PMID:19946146 IscS is the specific cysteine desulfurase transferring the sulfur to the MoaD protein. MetaCyc:RXN-12473 Source assertion: RXN-12473 consumes a cysteine-desulfurase persulfide and adenylated MoaD and produces thiocarboxylated MoaD. Source location `/ptools-xml/*[@frameid='RXN-12473']/left; right`
Thiosulfate sulfurtransferase YnjE `UniProtKB:P78067`	enables YnjE enhanced cPMP-to-MPT production in a defined IscS/MoeB/MoaD-containing reconstitution. This is an enhancing functional role in the net sulfur-transfer process, not proof of YnjE alone catalyzing the whole reaction or being universally required in vivo. No uniform cellular compartment is assigned.	[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 => [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+) `RHEA:48613`	PMID:21856748 YnjE was shown to enhance the rate of the conversion of added cyclic pyranopterin monophosphate to MPT. MetaCyc:G6952-MONOMER Source assertion: The YnjE frame links UniProt P78067 and its proposed MoaD sulfurtransferase role; primary PMID:21856748 is cited. Source location `/ptools-xml/*[@frameid='G6952-MONOMER']/dblink; catalyzes; comment`
Molybdopterin synthase sulfur carrier subunit `UniProtKB:P30748`	enables MoaD carries the sulfur incorporated into the pterin and cycles between free, adenylated and thiocarboxylated forms and the MoeB/MoaE assemblies. Whole Rhea carrier classes express these states; the unmodified sequence accession is not itself a chemically specific carrier state.	`MetaCyc:PWY-6823`	UniProtKB:P30748 Source assertion: MoaD participates in sulfur transfer from precursor Z to molybdopterin; ECO:0000269 (PMID:17223713). Source location `$.comments[0].texts` UniProtKB:P30748 Source assertion: MoaD shuttles between MoaD2-MoaE2 and MoaD2-MoeB2 complexes during catalysis; ECO:0000269 (PMID:11135669 and PMID:12571227). Source location `$.comments[2].texts`
molybdopterin synthase `MetaCyc:CPLX0-2502`	catalyzes The MoaD2-MoaE2 synthase incorporates two sulfur atoms into cPMP. The two thiocarboxylated MoaD equivalents are explicit, and the carrier is regenerated for another activation cycle.	2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + cyclic pyranopterin phosphate + H2O => molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 2 H(+) `RHEA:26334`	MetaCyc:CPLX0-2502 Source assertion: The MoaD/MoaE complex catalyzes RXN-8342. Source location `/ptools-xml/[@frameid='CPLX0-2502']/catalyzes/Enzymatic-Reaction/reaction` UniProtKB:P30749 Source assertion:* MoaE is annotated for RHEA:26333, consuming two thiocarboxylated MoaD equivalents; ECO:0000269 (PMID:12571226). Source location `$.comments[1].reaction` PMID:12571226 a dimer of dimers containing the MoaD and MoaE proteins
molybdopterin adenylyltransferase `MetaCyc:CPLX0-8163`	catalyzes Mog adenylates molybdopterin before molybdate insertion. ATP/magnesium assay conditions do not independently establish a separate bound-magnesium cofactor assignment for Mog.	molybdopterin + ATP + H(+) => adenylyl-molybdopterin + diphosphate `RHEA:31332`	MetaCyc:CPLX0-8163 Source assertion: The Mog complex catalyzes RXN-8344. Source location `/ptools-xml/[@frameid='CPLX0-8163']/catalyzes/Enzymatic-Reaction/reaction` UniProtKB:P0AF03 Source assertion:* P0AF03 is annotated for RHEA:31331 with ECO:0000269 (PMID:15632135). Source location `$.comments[1].reaction`
molybdopterin molybdotransferase `MetaCyc:CPLX0-8045`	catalyzes MoeA inserts molybdate into adenylyl-molybdopterin. The purified E. coli system used human apo-sulfite oxidase as a cofactor readout, not as the E. coli catalyst. This endpoint precedes guanylylation/cytidylylation and downstream cofactor loading.	adenylyl-molybdopterin + molybdate => Mo-molybdopterin + AMP + H(+) `RHEA:35048`	MetaCyc:CPLX0-8045 Source assertion: The MoeA complex catalyzes RXN-8348. Source location `/ptools-xml/[@frameid='CPLX0-8045']/catalyzes/Enzymatic-Reaction/reaction` UniProtKB:P12281 Source assertion:* P12281 is annotated for RHEA:35047 with ECO:0000269 (PMID:15632135). Source location `$.comments[1].reaction` PMID:15632135 Escherichia coli MoeA and MogA are required in vivo for the final step of molybdenum cofactor biosynthesis
Cyclic pyranopterin monophosphate synthase `UniProtKB:P0A738`	part_of Native assembly stoichiometry: 6 copies of this subunit. The native complex is curated for MG1655, within the selected K-12 scope. Transient catalytic states and alternative partner assemblies are described separately.	cyclic pyranopterin monophosphate synthase `MetaCyc:CPLX-8331`	MetaCyc:CPLX-8331 Source assertion: CPLX-8331 contains 6 copies of EG11666-MONOMER. Source location `/ptools-xml/[@frameid='CPLX-8331']/component[Protein/@frameid="EG11666-MONOMER"]/coefficient` MetaCyc:EG11666-MONOMER Source assertion:* EG11666-MONOMER links the selected E. coli protein to UniProt P0A738. Source location `/ptools-xml/[@frameid='EG11666-MONOMER']/dblink[dblink-db="UNIPROT"]` UniProtKB:P0A738 Source assertion:* The reviewed SUBUNIT comment supports the 6-copy P0A738 component in this assembly; its cited experimental evidence is retained. Source location `$.comments[4].texts` PMID:25941396 The hexameric structure (a trimer of homodimers)
Molybdopterin synthase sulfur carrier subunit `UniProtKB:P30748`	part_of Native assembly stoichiometry: 2 copies of this subunit. The native complex is curated for MG1655, within the selected K-12 scope. Transient catalytic states and alternative partner assemblies are described separately.	molybdopterin synthase `MetaCyc:CPLX0-2502`	MetaCyc:CPLX0-2502 Source assertion: CPLX0-2502 contains 2 copies of EG11597-MONOMER. Source location `/ptools-xml/[@frameid='CPLX0-2502']/component[Protein/@frameid="EG11597-MONOMER"]/coefficient` MetaCyc:EG11597-MONOMER Source assertion:* EG11597-MONOMER links the selected E. coli protein to UniProt P30748. Source location `/ptools-xml/[@frameid='EG11597-MONOMER']/dblink[dblink-db="UNIPROT"]` UniProtKB:P30748 Source assertion:* The reviewed SUBUNIT comment supports the 2-copy P30748 component in this assembly; its cited experimental evidence is retained. Source location `$.comments[2].texts`
Molybdopterin synthase catalytic subunit `UniProtKB:P30749`	part_of Native assembly stoichiometry: 2 copies of this subunit. The native complex is curated for MG1655, within the selected K-12 scope. Transient catalytic states and alternative partner assemblies are described separately.	molybdopterin synthase `MetaCyc:CPLX0-2502`	MetaCyc:CPLX0-2502 Source assertion: CPLX0-2502 contains 2 copies of EG11598-MONOMER. Source location `/ptools-xml/[@frameid='CPLX0-2502']/component[Protein/@frameid="EG11598-MONOMER"]/coefficient` MetaCyc:EG11598-MONOMER Source assertion:* EG11598-MONOMER links the selected E. coli protein to UniProt P30749. Source location `/ptools-xml/[@frameid='EG11598-MONOMER']/dblink[dblink-db="UNIPROT"]` UniProtKB:P30749 Source assertion:* The reviewed SUBUNIT comment supports the 2-copy P30749 component in this assembly; its cited experimental evidence is retained. Source location `$.comments[3].texts`
Molybdopterin-synthase adenylyltransferase `UniProtKB:P12282`	part_of Native assembly stoichiometry: 2 copies of this subunit. The native complex is curated for MG1655, within the selected K-12 scope. Transient catalytic states and alternative partner assemblies are described separately.	molybdopterin-synthase adenylyltransferase `MetaCyc:CPLX0-8164`	MetaCyc:CPLX0-8164 Source assertion: CPLX0-8164 contains 2 copies of EG10154-MONOMER. Source location `/ptools-xml/[@frameid='CPLX0-8164']/component[Protein/@frameid="EG10154-MONOMER"]/coefficient` MetaCyc:EG10154-MONOMER Source assertion:* EG10154-MONOMER links the selected E. coli protein to UniProt P12282. Source location `/ptools-xml/[@frameid='EG10154-MONOMER']/dblink[dblink-db="UNIPROT"]` UniProtKB:P12282 Source assertion:* The reviewed SUBUNIT comment supports the 2-copy P12282 component in this assembly; its cited experimental evidence is retained. Source location `$.comments[4].texts`
Molybdopterin adenylyltransferase `UniProtKB:P0AF03`	part_of Native assembly stoichiometry: 3 copies of this subunit. The native complex is curated for MG1655, within the selected K-12 scope. Transient catalytic states and alternative partner assemblies are described separately.	molybdopterin adenylyltransferase `MetaCyc:CPLX0-8163`	MetaCyc:CPLX0-8163 Source assertion: CPLX0-8163 contains 3 copies of EG11511-MONOMER. Source location `/ptools-xml/[@frameid='CPLX0-8163']/component[Protein/@frameid="EG11511-MONOMER"]/coefficient` MetaCyc:EG11511-MONOMER Source assertion:* EG11511-MONOMER links the selected E. coli protein to UniProt P0AF03. Source location `/ptools-xml/[@frameid='EG11511-MONOMER']/dblink[dblink-db="UNIPROT"]` UniProtKB:P0AF03 Source assertion:* The reviewed SUBUNIT comment supports the 3-copy P0AF03 component in this assembly; its cited experimental evidence is retained. Source location `$.comments[3].texts`
Molybdopterin molybdenumtransferase `UniProtKB:P12281`	part_of Native assembly stoichiometry: 2 copies of this subunit. The native complex is curated for MG1655, within the selected K-12 scope. Transient catalytic states and alternative partner assemblies are described separately.	molybdopterin molybdotransferase `MetaCyc:CPLX0-8045`	MetaCyc:CPLX0-8045 Source assertion: CPLX0-8045 contains 2 copies of EG10153-MONOMER. Source location `/ptools-xml/[@frameid='CPLX0-8045']/component[Protein/@frameid="EG10153-MONOMER"]/coefficient` MetaCyc:EG10153-MONOMER Source assertion:* EG10153-MONOMER links the selected E. coli protein to UniProt P12281. Source location `/ptools-xml/[@frameid='EG10153-MONOMER']/dblink[dblink-db="UNIPROT"]` UniProtKB:P12281 Source assertion:* The reviewed SUBUNIT comment supports the 2-copy P12281 component in this assembly; its cited experimental evidence is retained. Source location `$.comments[4].texts`
Cysteine desulfurase IscS `UniProtKB:P0A6B7`	part_of Native assembly stoichiometry: 2 copies of this subunit. The native complex is curated for MG1655, within the selected K-12 scope. Transient catalytic states and alternative partner assemblies are described separately.	cysteine desulfurase `MetaCyc:CPLX0-248`	MetaCyc:CPLX0-248 Source assertion: CPLX0-248 contains 2 copies of G7325-MONOMER. Source location `/ptools-xml/[@frameid='CPLX0-248']/component[Protein/@frameid="G7325-MONOMER"]/coefficient` MetaCyc:G7325-MONOMER Source assertion:* G7325-MONOMER links the selected E. coli protein to UniProt P0A6B7. Source location `/ptools-xml/[@frameid='G7325-MONOMER']/dblink[dblink-db="UNIPROT"]` UniProtKB:P0A6B7 Source assertion:* The reviewed SUBUNIT comment supports the 2-copy P0A6B7 component in this assembly; its cited experimental evidence is retained. Source location `$.comments[6].texts`
GTP 3',8-cyclase `UniProtKB:P30745`	has_cofactor Two [4Fe-4S] clusters are inferred for K-12 MoaA by HAMAP. SAM-dependent chemistry and oxygen-sensitive enzyme preparation are distinguished from a blanket anaerobic-growth requirement.	tetra-mu3-sulfido-tetrairon `CHEBI:49883`	UniProtKB:P30745 Source assertion: The cofactor comment assigns two [4Fe-4S] clusters, CHEBI:49883, with ECO:0000255 (HAMAP MF_01225). Source location `$.comments[3].cofactors; $.comments[3].note`
Molybdopterin-synthase adenylyltransferase `UniProtKB:P12282`	has_cofactor The reviewed MoeB annotation assigns one zinc ion per subunit. This is distinct from magnesium supplied with ATP in activation assays.	zinc(2+) `CHEBI:29105`	UniProtKB:P12282 Source assertion: The cofactor comment assigns CHEBI:29105, one zinc ion per subunit, with ECO:0000269 (PMID:11463785). Source location `$.comments[2].cofactors; $.comments[2].note`
Molybdopterin molybdenumtransferase `UniProtKB:P12281`	has_cofactor The reviewed MoeA annotation assigns one bound magnesium ion per subunit from structural evidence; it is not inferred merely from MgATP assay conditions.	magnesium(2+) `CHEBI:18420`	UniProtKB:P12281 Source assertion: The cofactor comment assigns CHEBI:18420, one magnesium ion per subunit, with ECO:0000269 (PMID:11428898). Source location `$.comments[2].cofactors; $.comments[2].note`
Cysteine desulfurase IscS `UniProtKB:P0A6B7`	has_cofactor IscS uses pyridoxal phosphate for cysteine desulfuration.	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P0A6B7 Source assertion: The cofactor comment explicitly identifies CHEBI:597326 with ECO:0000269 (PMID:10600118 and PMID:12860127) and HAMAP ECO:0000255. Source location `$.comments[2].cofactors`

References

RHEA:49576 Rhea reaction 49576 (source version: Rhea release 142, 2026-09-02; SHA-256 of the complete compressed RDF distribution)
RHEA:49580 Rhea reaction 49580 (source version: Rhea release 142, 2026-09-02; SHA-256 of the complete compressed RDF distribution)
RHEA:17457 Rhea reaction 17457 (source version: Rhea release 142, 2026-09-02; SHA-256 of the complete compressed RDF distribution)
RHEA:43616 Rhea reaction 43616 (source version: Rhea release 142, 2026-09-02; SHA-256 of the complete compressed RDF distribution)
RHEA:48612 Rhea reaction 48612 (source version: Rhea release 142, 2026-09-02; SHA-256 of the complete compressed RDF distribution)
RHEA:26333 Rhea reaction 26333 (source version: Rhea release 142, 2026-09-02; SHA-256 of the complete compressed RDF distribution)
RHEA:31331 Rhea reaction 31331 (source version: Rhea release 142, 2026-09-02; SHA-256 of the complete compressed RDF distribution)
RHEA:35047 Rhea reaction 35047 (source version: Rhea release 142, 2026-09-02; SHA-256 of the complete compressed RDF distribution)
UniProtKB:P30745 GTP 3',8-cyclase (source version: UniProtKB 2026_03; retrieved 2026-10-11)
UniProtKB:P0A738 Cyclic pyranopterin monophosphate synthase (source version: UniProtKB 2026_03; retrieved 2026-10-11)
UniProtKB:P30748 Molybdopterin synthase sulfur carrier subunit (source version: UniProtKB 2026_03; retrieved 2026-10-11)
UniProtKB:P30749 Molybdopterin synthase catalytic subunit (source version: UniProtKB 2026_03; retrieved 2026-10-11)
UniProtKB:P12281 Molybdopterin molybdenumtransferase (source version: UniProtKB 2026_03; retrieved 2026-10-11)
UniProtKB:P12282 Molybdopterin-synthase adenylyltransferase (source version: UniProtKB 2026_03; retrieved 2026-10-11)
UniProtKB:P0AF03 Molybdopterin adenylyltransferase (source version: UniProtKB 2026_03; retrieved 2026-10-11)
UniProtKB:P0A6B7 Cysteine desulfurase IscS (source version: UniProtKB 2026_03; retrieved 2026-10-11)
UniProtKB:P78067 Thiosulfate sulfurtransferase YnjE (source version: UniProtKB 2026_03; retrieved 2026-10-11)
MetaCyc:PWY-6823 molybdenum cofactor biosynthesis (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:RXN-8340 RXN-8340 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:RXN-17809 RXN-17809 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:RXN0-308 RXN0-308 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:RXN-11361 RXN-11361 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:RXN-12473 RXN-12473 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:RXN-8342 RXN-8342 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:RXN-8344 RXN-8344 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:RXN-8348 RXN-8348 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:CPLX-8331 cyclic pyranopterin monophosphate synthase (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:EG11666-MONOMER EG11666-MONOMER (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:CPLX0-2502 molybdopterin synthase (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:EG11597-MONOMER EG11597-MONOMER (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:EG11598-MONOMER EG11598-MONOMER (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:CPLX0-8164 molybdopterin-synthase adenylyltransferase (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:EG10154-MONOMER EG10154-MONOMER (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:CPLX0-8163 molybdopterin adenylyltransferase (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:EG11511-MONOMER EG11511-MONOMER (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:CPLX0-8045 molybdopterin molybdotransferase (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:EG10153-MONOMER EG10153-MONOMER (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:CPLX0-248 cysteine desulfurase (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:G7325-MONOMER G7325-MONOMER (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:EG11595-MONOMER EG11595-MONOMER (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:ENZRXN-19108 ENZRXN-19108 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:G6952-MONOMER G6952-MONOMER (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:CPD-19179 CPD-19179 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
MetaCyc:CPD-8123 CPD-8123 (source version: MetaCyc 22.5 native XML; retrieved 2026-10-11)
PMID:25941396 Mechanism of pyranopterin ring formation in molybdenum cofactor biosynthesis. (source version: PMC full article HTML; retrieved 2026-10-11; digest of actual HTML body)
PMID:19946146 IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli. (source version: PMC full article HTML; retrieved 2026-10-11; digest of actual HTML body)
PMID:21856748 The identification of a novel protein involved in molybdenum cofactor biosynthesis in Escherichia coli. (source version: Europe PMC indexed primary abstract and metadata; retrieved 2026-10-11; not a full-text digest)
PMID:12571226 Mechanistic and mutational studies of Escherichia coli molybdopterin synthase clarify the final step of molybdopterin biosynthesis. (source version: Europe PMC indexed primary abstract and metadata; retrieved 2026-10-11; not a full-text digest)
PMID:15632135 In vitro molybdenum ligation to molybdopterin using purified components. (source version: Europe PMC indexed primary abstract and metadata; retrieved 2026-10-11; not a full-text digest)
CHEBI:131766 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate(4-) (source version: ChEBI 255 Semantic SQL snapshot; SHA-256 of complete preserved database file)

Curation history

2026-10-11T02:29:44Z Codex
- CREATE — Add the complete native GTP-to-molybdenum-cofactor pathway.
  Retained all eight native reactions, sulfur-carrier activation cycle and source-defined protein assemblies. Separated PathBank collage, native versus current chemistry and supported YnjE enhancement. Corrected MoaA intermediate cross-reference source attribution under issue342. Source and duplicate-search dossiers are in research/pathway_additions/2026-10-11-nicotine-next; shared authorities, generated pages, final gates and independent reviews are recorded separately.
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