sulfoquinovose degradation II

MetaCyc:PWY-7722

The complete five-transformation sulfo-Entner-Doudoroff route from free sulfoquinovose to pyruvate and sulfolactate in Pseudomonas putida strain SQ1 (DSM 100120). Six exact Rhea activities represent the five native members because the final SLA oxidation has alternative NAD- and NADP-dependent forms. All five SQ1 enzymes were reconstituted in vitro and induced during aerobic SQ growth. Current native reaction structures and reviewed Rhea/UniProt assignments retain S-SLA/S-SL; older native protein prose linking R-SLA is superseded, without recasting the SQ1 HPLC-MS assay as an enantiomer-resolved experiment. SQDH has direct tetramer evidence; the other functional protein/activity relations do not assert monomer sufficiency. Metal annotations preserve inference levels, and the aldolase genomic-start difference is explicit. The endpoint retains organic sulfur. SQ-glycoside hydrolysis, mutarotation, unidentified transport/regulatory mechanisms, downstream pyruvate metabolism and partner-organism sulfolactate mineralization are outside this exact native boundary. KT2440 expression datasets do not establish SQ1 applicability.

Pathway type

sulfo ED catabolism

Organism scope

Pseudomonas putida NCBITaxon:303

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Pathway network

27 nodes · 48 asserted directed edges

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Reaction / activity
Chemical / cofactor
Protein / complex
Pathway
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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Pseudomonas putida `NCBITaxon:303`	taxon
pyruvate `CHEBI:15361`	small molecule
water `CHEBI:15377`	small molecule
hydron `CHEBI:15378`	small molecule
NAD(1-) `CHEBI:57540`	small molecule
NADPH(4-) `CHEBI:57783`	small molecule
NADH(2-) `CHEBI:57945`	small molecule
NADP(3-) `CHEBI:58349`	small molecule
(S)-3-sulfonatolactate(2-) `CHEBI:61289`	small molecule
6-sulfo-D-quinovose(1-) `CHEBI:77132`	small molecule
6-deoxy-6-sulfoglucono-1,5-lactone(1-) `CHEBI:88091`	small molecule
6-deoxy-6-sulfo-D-gluconate(2-) `CHEBI:88093`	small molecule
2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate(2-) `CHEBI:88094`	small molecule
L-3-sulfolactaldehyde(1-) `CHEBI:90109`	small molecule
divalent metal cation `CHEBI:60240`	cofactor
tetra-mu3-sulfido-tetrairon `CHEBI:49883`	cofactor
Sulfoquinovose 1-dehydrogenase `UniProtKB:P0DOV5`	protein
6-deoxy-6-sulfogluconolactonase `UniProtKB:P0DOV6`	protein
6-deoxy-6-sulfo-D-gluconate dehydratase `UniProtKB:P0DOV7`	protein
2-dehydro-3,6-dideoxy-6-sulfogluconate aldolase `UniProtKB:P0DOV8`	protein
3-sulfolactaldehyde dehydrogenase `UniProtKB:P0DOV9`	protein
6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) `RHEA:47897`	molecular activity	left to right
6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) `RHEA:47909`	molecular activity	left to right
6-deoxy-6-sulfo-D-gluconate => 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O `RHEA:47913`	molecular activity	left to right
2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate `RHEA:47925`	molecular activity	left to right
(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	molecular activity	left to right
(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
6-sulfo-D-quinovose(1-) `CHEBI:77132`	consumes	6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) `RHEA:47897`	RHEA:47897 Source assertion: RHEA:47897 assigns CHEBI:77132 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47897"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47896_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47896_compound_9101"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_9101)`
NAD(1-) `CHEBI:57540`	consumes	6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) `RHEA:47897`	RHEA:47897 Source assertion: RHEA:47897 assigns CHEBI:57540 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47897"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47896_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47896_compound_3484"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_3484)`
6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) `RHEA:47897`	produces	6-deoxy-6-sulfoglucono-1,5-lactone(1-) `CHEBI:88091`	RHEA:47897 Source assertion: RHEA:47897 assigns CHEBI:88091 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47897"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47896_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47896_compound_11949"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11949)`
6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) `RHEA:47897`	produces	NADH(2-) `CHEBI:57945`	RHEA:47897 Source assertion: RHEA:47897 assigns CHEBI:57945 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47897"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47896_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47896_compound_1650"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_1650)`
6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) `RHEA:47897`	produces	hydron `CHEBI:15378`	RHEA:47897 Source assertion: RHEA:47897 assigns CHEBI:15378 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47897"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47896_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47896_compound_3249"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_3249)`
6-deoxy-6-sulfoglucono-1,5-lactone(1-) `CHEBI:88091`	consumes	6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) `RHEA:47909`	RHEA:47909 Source assertion: RHEA:47909 assigns CHEBI:88091 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47909"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47908_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47908_compound_11949"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11949)`
water `CHEBI:15377`	consumes	6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) `RHEA:47909`	RHEA:47909 Source assertion: RHEA:47909 assigns CHEBI:15377 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47909"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47908_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47908_compound_1283"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_1283)`
6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) `RHEA:47909`	produces	6-deoxy-6-sulfo-D-gluconate(2-) `CHEBI:88093`	RHEA:47909 Source assertion: RHEA:47909 assigns CHEBI:88093 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47909"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47908_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47908_compound_11950"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11950)`
6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) `RHEA:47909`	produces	hydron `CHEBI:15378`	RHEA:47909 Source assertion: RHEA:47909 assigns CHEBI:15378 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47909"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47908_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47908_compound_3249"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_3249)`
6-deoxy-6-sulfo-D-gluconate(2-) `CHEBI:88093`	consumes	6-deoxy-6-sulfo-D-gluconate => 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O `RHEA:47913`	RHEA:47913 Source assertion: RHEA:47913 assigns CHEBI:88093 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47913"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47912_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47912_compound_11950"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11950)`
6-deoxy-6-sulfo-D-gluconate => 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O `RHEA:47913`	produces	2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate(2-) `CHEBI:88094`	RHEA:47913 Source assertion: RHEA:47913 assigns CHEBI:88094 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47913"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47912_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47912_compound_11951"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11951)`
6-deoxy-6-sulfo-D-gluconate => 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O `RHEA:47913`	produces	water `CHEBI:15377`	RHEA:47913 Source assertion: RHEA:47913 assigns CHEBI:15377 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47913"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47912_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47912_compound_1283"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_1283)`
2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate(2-) `CHEBI:88094`	consumes	2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate `RHEA:47925`	RHEA:47925 Source assertion: RHEA:47925 assigns CHEBI:88094 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47925"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47924_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47924_compound_11951"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11951)`
2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate `RHEA:47925`	produces	L-3-sulfolactaldehyde(1-) `CHEBI:90109`	RHEA:47925 Source assertion: RHEA:47925 assigns CHEBI:90109 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47925"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47924_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47924_compound_12045"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_12045)`
2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate `RHEA:47925`	produces	pyruvate `CHEBI:15361`	RHEA:47925 Source assertion: RHEA:47925 assigns CHEBI:15361 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47925"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47924_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47924_compound_3719"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_3719)`
L-3-sulfolactaldehyde(1-) `CHEBI:90109`	consumes	(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	RHEA:47929 Source assertion: RHEA:47929 assigns CHEBI:90109 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47929"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47928_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47928_compound_12045"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_12045)`
NADP(3-) `CHEBI:58349`	consumes	(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	RHEA:47929 Source assertion: RHEA:47929 assigns CHEBI:58349 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47929"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47928_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47928_compound_5188"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_5188)`
water `CHEBI:15377`	consumes	(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	RHEA:47929 Source assertion: RHEA:47929 assigns CHEBI:15377 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47929"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47928_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47928_compound_1283"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_1283)`
(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	produces	(S)-3-sulfonatolactate(2-) `CHEBI:61289`	RHEA:47929 Source assertion: RHEA:47929 assigns CHEBI:61289 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47929"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47928_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47928_compound_6369"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_6369)`
(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	produces	NADPH(4-) `CHEBI:57783`	RHEA:47929 Source assertion: RHEA:47929 assigns CHEBI:57783 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47929"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47928_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47928_compound_3438"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_3438)`
(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	produces	hydron `CHEBI:15378`	RHEA:47929 Source assertion: RHEA:47929 assigns CHEBI:15378 to its products with coefficient 2. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47929"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47928_R"]/rh:contains2[@rdf:resource="http://rdf.rhea-db.org/Participant_47928_compound_3249"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_3249)`
L-3-sulfolactaldehyde(1-) `CHEBI:90109`	consumes	(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	RHEA:47933 Source assertion: RHEA:47933 assigns CHEBI:90109 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47933"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47932_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47932_compound_12045"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_12045)`
NAD(1-) `CHEBI:57540`	consumes	(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	RHEA:47933 Source assertion: RHEA:47933 assigns CHEBI:57540 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47933"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47932_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47932_compound_3484"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_3484)`
water `CHEBI:15377`	consumes	(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	RHEA:47933 Source assertion: RHEA:47933 assigns CHEBI:15377 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47933"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47932_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47932_compound_1283"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_1283)`
(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	produces	(S)-3-sulfonatolactate(2-) `CHEBI:61289`	RHEA:47933 Source assertion: RHEA:47933 assigns CHEBI:61289 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47933"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47932_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47932_compound_6369"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_6369)`
(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	produces	NADH(2-) `CHEBI:57945`	RHEA:47933 Source assertion: RHEA:47933 assigns CHEBI:57945 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47933"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47932_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47932_compound_1650"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_1650)`
(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	produces	hydron `CHEBI:15378`	RHEA:47933 Source assertion: RHEA:47933 assigns CHEBI:15378 to its products with coefficient 2. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47933"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47932_R"]/rh:contains2[@rdf:resource="http://rdf.rhea-db.org/Participant_47932_compound_3249"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_3249)`
6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) `RHEA:47897`	part_of	`MetaCyc:PWY-7722`	MetaCyc:PWY-7722 Source assertion: The complete native pathway lists RXN-17096 among its five reaction members. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-list/Reaction[@frameid="RXN-17096"]` MetaCyc:RXN-17096 Source assertion: The native reaction cross-references RHEA:47897 and records physiological left-to-right orientation. Source location `/ptools-xml/Reaction[@frameid="RXN-17096"]/dblink[dblink-db="RHEA"]/dblink-oid; /ptools-xml/Reaction/reaction-direction` RHEA:47897 Source assertion: Rhea master 47896 explicitly has directional reaction 47897; its left-to-right substrate and product assignment is used here. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47896"]/rh:directionalReaction[@rdf:resource="http://rdf.rhea-db.org/47897"]; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47897"]/rh:substrates and rh:products`
6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) `RHEA:47909`	part_of	`MetaCyc:PWY-7722`	MetaCyc:PWY-7722 Source assertion: The complete native pathway lists RXN-17095 among its five reaction members. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-list/Reaction[@frameid="RXN-17095"]` MetaCyc:RXN-17095 Source assertion: The native reaction cross-references RHEA:47909 and records physiological left-to-right orientation. Source location `/ptools-xml/Reaction[@frameid="RXN-17095"]/dblink[dblink-db="RHEA"]/dblink-oid; /ptools-xml/Reaction/reaction-direction` RHEA:47909 Source assertion: Rhea master 47908 explicitly has directional reaction 47909; its left-to-right substrate and product assignment is used here. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47908"]/rh:directionalReaction[@rdf:resource="http://rdf.rhea-db.org/47909"]; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47909"]/rh:substrates and rh:products`
6-deoxy-6-sulfo-D-gluconate => 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O `RHEA:47913`	part_of	`MetaCyc:PWY-7722`	MetaCyc:PWY-7722 Source assertion: The complete native pathway lists RXN-17097 among its five reaction members. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-list/Reaction[@frameid="RXN-17097"]` MetaCyc:RXN-17097 Source assertion: The native reaction cross-references RHEA:47913 and records physiological left-to-right orientation. Source location `/ptools-xml/Reaction[@frameid="RXN-17097"]/dblink[dblink-db="RHEA"]/dblink-oid; /ptools-xml/Reaction/reaction-direction` RHEA:47913 Source assertion: Rhea master 47912 explicitly has directional reaction 47913; its left-to-right substrate and product assignment is used here. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47912"]/rh:directionalReaction[@rdf:resource="http://rdf.rhea-db.org/47913"]; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47913"]/rh:substrates and rh:products`
2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate `RHEA:47925`	part_of	`MetaCyc:PWY-7722`	MetaCyc:PWY-7722 Source assertion: The complete native pathway lists RXN-17098 among its five reaction members. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-list/Reaction[@frameid="RXN-17098"]` MetaCyc:RXN-17098 Source assertion: The native reaction cross-references RHEA:47925 and records physiological left-to-right orientation. Source location `/ptools-xml/Reaction[@frameid="RXN-17098"]/dblink[dblink-db="RHEA"]/dblink-oid; /ptools-xml/Reaction/reaction-direction` RHEA:47925 Source assertion: Rhea master 47924 explicitly has directional reaction 47925; its left-to-right substrate and product assignment is used here. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47924"]/rh:directionalReaction[@rdf:resource="http://rdf.rhea-db.org/47925"]; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47925"]/rh:substrates and rh:products`
(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	part_of	`MetaCyc:PWY-7722`	MetaCyc:PWY-7722 Source assertion: The complete native pathway lists RXN-17101 among its five reaction members. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-list/Reaction[@frameid="RXN-17101"]` MetaCyc:RXN-17101 Source assertion: The native final reaction uses generic NAD(P)+ and NAD(P)H classes and links RHEA:47929. Its complete cofactor scope is represented by alternative NADP- and NAD-dependent activities, not two consecutive oxidations. Source location `/ptools-xml/Reaction[@frameid="RXN-17101"]/left; /ptools-xml/Reaction/right; /ptools-xml/Reaction/dblink[dblink-db="RHEA"]/dblink-oid` UniProtKB:P0DOV9 Source assertion: ECO:0000269 (PMID26195800): the reviewed SQ1 protein explicitly assigns the NADP-dependent activity to RHEA:47928. Source location `/comments/1/reaction/name; /comments/1/reaction/reactionCrossReferences/0; /comments/1/reaction/evidences` RHEA:47929 Source assertion: Rhea master 47928 explicitly has directional reaction 47929; its left-to-right substrate and product assignment is used here. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47928"]/rh:directionalReaction[@rdf:resource="http://rdf.rhea-db.org/47929"]; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47929"]/rh:substrates and rh:products`
(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	part_of	`MetaCyc:PWY-7722`	MetaCyc:PWY-7722 Source assertion: The complete native pathway lists RXN-17101 among its five reaction members. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-list/Reaction[@frameid="RXN-17101"]` MetaCyc:RXN-17101 Source assertion: The native final reaction uses generic NAD(P)+ and NAD(P)H classes and links RHEA:47929. Its complete cofactor scope is represented by alternative NADP- and NAD-dependent activities, not two consecutive oxidations. Source location `/ptools-xml/Reaction[@frameid="RXN-17101"]/left; /ptools-xml/Reaction/right; /ptools-xml/Reaction/dblink[dblink-db="RHEA"]/dblink-oid` UniProtKB:P0DOV9 Source assertion: ECO:0000269 (PMID26195800): the reviewed SQ1 protein explicitly assigns the NAD-dependent activity to RHEA:47932. Source location `/comments/2/reaction/name; /comments/2/reaction/reactionCrossReferences/0; /comments/2/reaction/evidences` RHEA:47933 Source assertion: Rhea master 47932 explicitly has directional reaction 47933; its left-to-right substrate and product assignment is used here. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47932"]/rh:directionalReaction[@rdf:resource="http://rdf.rhea-db.org/47933"]; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47933"]/rh:substrates and rh:products`
Sulfoquinovose 1-dehydrogenase `UniProtKB:P0DOV5`	enables Functional protein/activity relation, not monomer sufficiency. The 2025 recombinant SQ1 enzyme is tetrameric in solution and crystals. Minor NADP activity and trace G6P activity are assay-dependent side activities outside the native NAD-dependent step.	6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) `RHEA:47897`	UniProtKB:P0DOV5 Source assertion: ECO:0000269, PMID26195800: this reviewed SQ1 gene product is assigned the specified catalytic activity; its cited genomic and experimental references explicitly name strain SQ1. Source location `/comments/1/reaction; /references/0/referenceComments; /references/1/referenceComments` PMID:26195800 Source assertion: SQ1 IMG0090 was cloned and purified from recombinant E. coli; it oxidized SQ to SGL with a strong NAD+ preference. Whole-pathway reconstitution used the purified protein. Source location `//[@id="s4"]/p[1]; //[@id="s5"]/p[2]; Figure 4B; SI Heterologous Overproduction and Purification of His-Tagged Proteins` PMID:39840830 Source assertion: The codon-harmonized SQ1 SQDH construct has a tetrameric solution state by SEC-MALLS (~119 kDa versus ~28 kDa monomer), consistent with its crystals. The published untagged sequence equals P0DOV5. Source location `//[@id="s2-2"]/p[1]; Figure 4; //[@id="s5-2"]; //[@id="s5-3"]/p[1]` PMID:39840830 Source assertion:* Under the 2025 kinetic conditions, NAD+ was favored over NADP+ by about 10^6 in catalytic efficiency, and very weak G6P turnover was detectable at high enzyme concentration; the 2015 negative assay is condition-specific. Source location `//[@id="s2-1"]/p[1]; //[@id="s2-1"]/p[2]; Table 1`
6-deoxy-6-sulfogluconolactonase `UniProtKB:P0DOV6`	enables Functional activity assignment. Divalent-metal binding below is a reviewed similarity inference, not an SQ1 metal-titration measurement. No physical monomer or oligomer state is inferred from the native MONOMER frame name.	6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) `RHEA:47909`	UniProtKB:P0DOV6 Source assertion: ECO:0000269, PMID26195800: this reviewed SQ1 gene product is assigned the specified catalytic activity; its cited genomic and experimental references explicitly name strain SQ1. Source location `/comments/1/reaction; /references/0/referenceComments; /references/1/referenceComments` PMID:26195800 Source assertion: Purified recombinant SQ1 IMG0091 converted SGL to SG in sequential reconstitution. Minor spontaneous hydrolysis was also observed before lactonase addition; it does not replace the enzyme-supported native step. Source location `//[@id="s5"]/p[1]; //[@id="s5"]/p[2]; Figure 4B-C`
6-deoxy-6-sulfo-D-gluconate dehydratase `UniProtKB:P0DOV7`	enables Functional activity assignment; current reviewed homodimer and one [4Fe-4S] cluster annotations are inferred by similarity to Q1JUQ1, not directly measured SQ1 assembly or cluster occupancy.	6-deoxy-6-sulfo-D-gluconate => 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O `RHEA:47913`	UniProtKB:P0DOV7 Source assertion: ECO:0000269, PMID26195800: this reviewed SQ1 gene product is assigned the specified catalytic activity; its cited genomic and experimental references explicitly name strain SQ1. Source location `/comments/1/reaction; /references/0/referenceComments; /references/1/referenceComments` PMID:26195800 Source assertion: Purified recombinant SQ1 IMG0089 converted SG to KDSG in the sequential reconstitution; its preparation also contained C-terminally truncated products identified by peptide fingerprinting. Source location `//[@id="s5"]/p[1]; //[@id="s5"]/p[2]; Figure 4C-D; Figure S4 legend` UniProtKB:P0DOV7 Source assertion: The homodimer annotation is ECO:0000250 inference from Q1JUQ1. It is not direct experimental assembly evidence for SQ1. Source location `/comments/3/texts/0`
2-dehydro-3,6-dideoxy-6-sulfogluconate aldolase `UniProtKB:P0DOV8`	enables Functional assignment of the reviewed SQ1 gene product. The 2015 cloned IMG00100 gene uses an upstream start relative to the current GenBank annotation at the same locus. Native prose has stale R-SLA/family annotations; current native reaction and Rhea/UniProt chemistry select S-SLA. No monomer sufficiency is asserted.	2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate `RHEA:47925`	UniProtKB:P0DOV8 Source assertion: ECO:0000269, PMID26195800: this reviewed SQ1 gene product is assigned the specified catalytic activity; its cited genomic and experimental references explicitly name strain SQ1. Source location `/comments/1/reaction; /references/0/referenceComments; /references/1/referenceComments` PMID:26195800 Source assertion: Purified recombinant SQ1 IMG0100 cleaved KDSG to SLA and pyruvate. Pyruvate was verified by a positive lactate-dehydrogenase assay and HPLC cochromatography; the SLA assay was not enantiomer-resolved. Source location `//[@id="s5"]/p[1]; //[@id="s5"]/p[2]; Figure 4D-E` PMID:26195800 Source assertion: The cloned IMG00100 used forward primer CACCATGTCCACTGCTGTCACG. All recombinant inserts came from SQ1 chromosomal DNA and were sequence-verified; the paper does not specify current UniProt accessions. Source location `//[@id="si8"]/p[1]; Table S2, PpSQ1_00100f row` NCBIProtein:KHL76360.1 Source assertion:* The original SQ1 protein record locates PpSQ1_00455 at complement(JTCJ01000004.1:18793..19560). This is the current annotated gene call, not a claim that its N terminus equals the longer 2015 construct. Source location `/GBSet/GBSeq/GBSeq_feature-table/GBFeature[GBFeature_key="source"]/GBFeature_quals; /GBSet/GBSeq/GBSeq_feature-table/GBFeature[GBFeature_key="CDS"]/GBFeature_quals; /GBSet/GBSeq/GBSeq_sequence` PMID:41830472 Source assertion: The 2026 P. putida aldolase abstract reports a homohexamer, optimal Co/Mn activity and modest KDPG cross-reactivity. The accessible abstract does not expose its strain or construct sequence; these details are contextual and are not transferred as direct SQ1 assembly/metal assignments. Source location `/resultList/result[0]/abstractText`
3-sulfolactaldehyde dehydrogenase `UniProtKB:P0DOV9`	enables Alternative NADP- and NAD-dependent final activity, not sequential steps. Current Rhea/UniProt and native reaction sides select S-SLA/S-SL; stale native protein prose links R-SLA. The SQ1 HPLC-MS assay was not an independent enantiomer-resolved characterization.	(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	UniProtKB:P0DOV9 Source assertion: ECO:0000269, PMID26195800: this reviewed SQ1 gene product is assigned the specified catalytic activity; its cited genomic and experimental references explicitly name strain SQ1. Source location `/comments/1/reaction; /references/0/referenceComments; /references/1/referenceComments` PMID:26195800 Source assertion: Purified recombinant SQ1 IMG0088 oxidized SLA to SL with both NAD+ and NADP+. Published kinetic constants used succinate semialdehyde because the SLA concentration was undefined; no SLA Km is assigned here. Source location `//[@id="s4"]/p[2]; Table 1 and footnote; Figure 4E-F` PMID:37886098 Source assertion:* The later tetramer and stereoselectivity experiments concern Rhizobium leguminosarum SRDI565 RlGabD, not SQ1. They are not transferred as direct assembly or enantiomer-assay evidence for P0DOV9. Source location `//[@id="abstract1"]/p[1]; //[@id="sec3"]/p[1]; //*[@id="sec6"]/p[1]`
3-sulfolactaldehyde dehydrogenase `UniProtKB:P0DOV9`	enables Alternative NADP- and NAD-dependent final activity, not sequential steps. Current Rhea/UniProt and native reaction sides select S-SLA/S-SL; stale native protein prose links R-SLA. The SQ1 HPLC-MS assay was not an independent enantiomer-resolved characterization.	(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	UniProtKB:P0DOV9 Source assertion: ECO:0000269, PMID26195800: this reviewed SQ1 gene product is assigned the specified catalytic activity; its cited genomic and experimental references explicitly name strain SQ1. Source location `/comments/2/reaction; /references/0/referenceComments; /references/1/referenceComments` PMID:26195800 Source assertion: Purified recombinant SQ1 IMG0088 oxidized SLA to SL with both NAD+ and NADP+. Published kinetic constants used succinate semialdehyde because the SLA concentration was undefined; no SLA Km is assigned here. Source location `//[@id="s4"]/p[2]; Table 1 and footnote; Figure 4E-F` PMID:37886098 Source assertion:* The later tetramer and stereoselectivity experiments concern Rhizobium leguminosarum SRDI565 RlGabD, not SQ1. They are not transferred as direct assembly or enantiomer-assay evidence for P0DOV9. Source location `//[@id="abstract1"]/p[1]; //[@id="sec3"]/p[1]; //*[@id="sec6"]/p[1]`
6-deoxy-6-sulfogluconolactonase `UniProtKB:P0DOV6`	has_cofactor	divalent metal cation `CHEBI:60240`	UniProtKB:P0DOV6 Source assertion: ECO:0000250 inference from Q15493: the reviewed entry assigns one divalent metal cation per subunit. This is a similarity annotation, not direct SQ1 metal identity or occupancy measurement. Source location `/comments/2/cofactors/0; /comments/2/note/texts/0`
6-deoxy-6-sulfo-D-gluconate dehydratase `UniProtKB:P0DOV7`	has_cofactor	tetra-mu3-sulfido-tetrairon `CHEBI:49883`	UniProtKB:P0DOV7 Source assertion: ECO:0000250 inference from Q1JUQ1: the reviewed entry assigns one [4Fe-4S] cluster. This is a similarity annotation, not direct SQ1 metal identity or occupancy measurement. Source location `/comments/2/cofactors/0; /comments/2/note/texts/0`
2-dehydro-3,6-dideoxy-6-sulfogluconate aldolase `UniProtKB:P0DOV8`	has_cofactor	divalent metal cation `CHEBI:60240`	UniProtKB:P0DOV8 Source assertion: ECO:0000250 inference from Q47098: the reviewed entry assigns one divalent metal cation per subunit. This is a similarity annotation, not direct SQ1 metal identity or occupancy measurement. Source location `/comments/2/cofactors/0; /comments/2/note/texts/0`
6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) `RHEA:47897`	provides_input_for	6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) `RHEA:47909`	MetaCyc:PWY-7722 Source assertion: The native predecessor list places RXN-17096 before RXN-17095. This citation supports native order; the shared current chemical identity is separately grounded in Rhea. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-ordering[Reaction/@frameid="RXN-17095"]/predecessor-reactions/Reaction[@frameid="RXN-17096"]` RHEA:47897 Source assertion: RHEA:47897 assigns CHEBI:88091 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47897"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47896_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47896_compound_11949"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11949)` RHEA:47909 Source assertion: RHEA:47909 assigns CHEBI:88091 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47909"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47908_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47908_compound_11949"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11949)`
6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) `RHEA:47909`	provides_input_for	6-deoxy-6-sulfo-D-gluconate => 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O `RHEA:47913`	MetaCyc:PWY-7722 Source assertion: The native predecessor list places RXN-17095 before RXN-17097. This citation supports native order; the shared current chemical identity is separately grounded in Rhea. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-ordering[Reaction/@frameid="RXN-17097"]/predecessor-reactions/Reaction[@frameid="RXN-17095"]` RHEA:47909 Source assertion: RHEA:47909 assigns CHEBI:88093 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47909"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47908_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47908_compound_11950"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11950)` RHEA:47913 Source assertion: RHEA:47913 assigns CHEBI:88093 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47913"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47912_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47912_compound_11950"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11950)`
6-deoxy-6-sulfo-D-gluconate => 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O `RHEA:47913`	provides_input_for	2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate `RHEA:47925`	MetaCyc:PWY-7722 Source assertion: The native predecessor list places RXN-17097 before RXN-17098. This citation supports native order; the shared current chemical identity is separately grounded in Rhea. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-ordering[Reaction/@frameid="RXN-17098"]/predecessor-reactions/Reaction[@frameid="RXN-17097"]` RHEA:47913 Source assertion: RHEA:47913 assigns CHEBI:88094 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47913"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47912_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47912_compound_11951"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11951)` RHEA:47925 Source assertion: RHEA:47925 assigns CHEBI:88094 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47925"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47924_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47924_compound_11951"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_11951)`
2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate `RHEA:47925`	provides_input_for	(2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) `RHEA:47929`	MetaCyc:PWY-7722 Source assertion: The native predecessor list places RXN-17098 before RXN-17101. This citation supports native order; the shared current chemical identity is separately grounded in Rhea. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-ordering[Reaction/@frameid="RXN-17101"]/predecessor-reactions/Reaction[@frameid="RXN-17098"]` RHEA:47925 Source assertion: RHEA:47925 assigns CHEBI:90109 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47925"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47924_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47924_compound_12045"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_12045)` RHEA:47929 Source assertion: RHEA:47929 assigns CHEBI:90109 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47929"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47928_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47928_compound_12045"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_12045)`
2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate `RHEA:47925`	provides_input_for	(2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) `RHEA:47933`	MetaCyc:PWY-7722 Source assertion: The native predecessor list places RXN-17098 before RXN-17101. This citation supports native order; the shared current chemical identity is separately grounded in Rhea. Source location `/ptools-xml/Pathway[@frameid="PWY-7722"]/reaction-ordering[Reaction/@frameid="RXN-17101"]/predecessor-reactions/Reaction[@frameid="RXN-17098"]` RHEA:47925 Source assertion: RHEA:47925 assigns CHEBI:90109 to its products with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47925"]/rh:products; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47924_R"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47924_compound_12045"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_12045)` RHEA:47933 Source assertion: RHEA:47933 assigns CHEBI:90109 to its substrates with coefficient 1. Source location `rdf:Description[@rdf:about="http://rdf.rhea-db.org/47933"]/rh:substrates; rdf:Description[@rdf:about="http://rdf.rhea-db.org/47932_L"]/rh:contains1[@rdf:resource="http://rdf.rhea-db.org/Participant_47932_compound_12045"]; rh:compound/rh:chebi (http://rdf.rhea-db.org/Compound_12045)`
NADH(2-) `CHEBI:57945`	inhibits Conditional recombinant-enzyme inhibition; no cellular regulation or new transcription-factor identity is inferred.	Sulfoquinovose 1-dehydrogenase `UniProtKB:P0DOV5`	PMID:39840830 Source assertion: NADH competitively inhibits NAD+ in the recombinant SQDH product-inhibition assays, supporting an ordered mechanism with NAD+ binding first and NADH leaving last. This is assay-level product inhibition, not a measured cellular flux threshold. Source location `//*[@id="s2-1"]/p[4]; Figure 3; section 5.5 Enzyme kinetics`

References

MetaCyc:PWY-7722 sulfoquinovose degradation II (source version: MetaCyc 22.5 public mirror; full original XML retrieved 2026-10-11)
MetaCyc:RXN-17096 RXN-17096 (source version: MetaCyc 22.5 public mirror; full original XML retrieved 2026-10-11)
MetaCyc:RXN-17095 RXN-17095 (source version: MetaCyc 22.5 public mirror; full original XML retrieved 2026-10-11)
MetaCyc:RXN-17097 RXN-17097 (source version: MetaCyc 22.5 public mirror; full original XML retrieved 2026-10-11)
MetaCyc:RXN-17098 RXN-17098 (source version: MetaCyc 22.5 public mirror; full original XML retrieved 2026-10-11)
MetaCyc:RXN-17101 RXN-17101 (source version: MetaCyc 22.5 public mirror; full original XML retrieved 2026-10-11)
UniProtKB:P0DOV5 Sulfoquinovose 1-dehydrogenase (source version: Complete reviewed entry JSON retrieved 2026-10-11; entry version 25, sequence version 1; last annotation 2026-09-02)
UniProtKB:P0DOV6 6-deoxy-6-sulfogluconolactonase (source version: Complete reviewed entry JSON retrieved 2026-10-11; entry version 21, sequence version 1; last annotation 2026-06-10)
UniProtKB:P0DOV7 6-deoxy-6-sulfo-D-gluconate dehydratase (source version: Complete reviewed entry JSON retrieved 2026-10-11; entry version 26, sequence version 1; last annotation 2026-09-02)
UniProtKB:P0DOV8 2-dehydro-3,6-dideoxy-6-sulfogluconate aldolase (source version: Complete reviewed entry JSON retrieved 2026-10-11; entry version 21, sequence version 1; last annotation 2026-09-02)
UniProtKB:P0DOV9 3-sulfolactaldehyde dehydrogenase (source version: Complete reviewed entry JSON retrieved 2026-10-11; entry version 25, sequence version 1; last annotation 2026-09-02)
PMID:26195800 Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1. (source version: Original 2015 full primary article including SI methods, tables and figure legends in publisher-deposited PMC HTML; retrieved 2026-10-11)
PMID:39840830 Structure, kinetics, and mechanism of Pseudomonas putida sulfoquinovose dehydrogenase, the first enzyme in the sulfoglycolytic Entner-Doudoroff pathway. (source version: Original 2025 full primary PMC HTML, including complete construct sequences and Methods; retrieved 2026-10-11)
PMID:37886098 Molecular basis of sulfolactate synthesis by sulfolactaldehyde dehydrogenase from Rhizobium leguminosarum. (source version: Original 2023 primary full PMC HTML; other-organism scope, inspected as a transfer limit; retrieved 2026-10-11)
PMID:41830472 Metal-Dependent 2-Keto-3,6-dideoxy-6-sulfo-gluconate (KDSG) Aldolase: Decoding the Key C-C Bond Cleaving Step in Bacterial Sulfoglycolysis. (source version: Original 2026 primary abstract only, through Europe PMC single-PMID core JSON; full article/SI unavailable and deposited structure API requests returned404; retrieved 2026-10-11)
NCBIProtein:KHL76360.1 SQ1 PpSQ1_00455 protein and genomic CDS annotation (source version: Original accession-version GBSet/GBSeq XML retrieved 2026-10-11; sequence and strain/CDS identity only, automated original functional name not imported)
RHEA:47897 6-sulfo-D-quinovose + NAD(+) => 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H(+) (source version: Rhea 142, 2026-09-02; digest covers full original compressed RDF gzip)
RHEA:47909 6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O => 6-deoxy-6-sulfo-D-gluconate + H(+) (source version: Rhea 142, 2026-09-02; digest covers full original compressed RDF gzip)
RHEA:47913 6-deoxy-6-sulfo-D-gluconate => 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O (source version: Rhea 142, 2026-09-02; digest covers full original compressed RDF gzip)
RHEA:47925 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate => (2S)-3-sulfolactaldehyde + pyruvate (source version: Rhea 142, 2026-09-02; digest covers full original compressed RDF gzip)
RHEA:47929 (2S)-3-sulfolactaldehyde + NADP(+) + H2O => (2S)-3-sulfolactate + NADPH + 2 H(+) (source version: Rhea 142, 2026-09-02; digest covers full original compressed RDF gzip)
RHEA:47933 (2S)-3-sulfolactaldehyde + NAD(+) + H2O => (2S)-3-sulfolactate + NADH + 2 H(+) (source version: Rhea 142, 2026-09-02; digest covers full original compressed RDF gzip)

Curation history

2026-10-11T09:04:19Z codex
- CREATE — Add sulfoquinovose degradation II
  Curated all five native sulfo-ED steps with six cofactor-specific Rhea activities, exact SQ1 protein/construct scope and chemical stereochemistry qualifications. Maintained record MetaCyc:PWY-7722; creation bytes SHA256 20a95c209243399b88a90fbecb0d8d310ebfb3094fc6ee4487352fdc2185c596. Original-source projections, replay and identifier decisions are in research/pathway_additions/2026-10-11-next-203/. Duplicate searches included ignored and hidden files. Independent review and full integration gates are separate subsequent checks, not claimed by this creation event.
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