staphyloferrin B biosynthesis

MetaCyc:PWY-8008

Complete seven-transformation staphyloferrin B biosynthesis: SbnI/A/B supply two L-2,3-diaminopropionate units, followed by SbnE/H/F/C assembly with supplied citrate. The experimentally grounded implementation uses Staphylococcus aureus Newman proteins; NCTC 8325 proteins and native SbnI/B complexes are retained separately as source-defined assembly context, not renamed Newman entities. The seven selected current Rhea equations specify ionic forms and a formal open-chain terminal product. CHEBI:136993 is the linear dianion, not the cyclic hemiaminal characterized in the 2015 original supplement. That supplement reports synthetic SB as an equilibrating pair of hemiaminal epimers; PubChem CID 122234488 independently issues the neutral cyclic structure with an unspecified additional center. No exact equivalence or linear-only physiological endpoint is claimed. The observed ring-chain chemistry is disclosed without inventing an eighth reaction. Native citryl intermediates leave a stereocenter unspecified; current ChEBI S-specific forms are curated conventions rather than direct stereochemical measurements on every Newman intermediate. The five-ATP-per-formal-product total is derived from twice-turning precursor reactions and three ligations, not a measured cellular yield. SbnB reversibility is experimentally observed, while the selected direction follows biosynthetic flux. SbnI dimer/tetramer observations coexist with an active truncated monomer; SbnA/H dimers and SbnC/E/F monomer-dimer mixtures do not establish unique assembly sufficiency. Primary engineered constructs are distinguished from native sequence identities. The 2009 reconstitution uses Newman coding regions and RN6390 genetic comparison; the 2015 biological assays use USA300 JE2. Native DAP and ACEGA protonation differs from the selected Rhea forms; equations are not asserted to be exactly identical. Source-native proton/mass metadata conflicts are retained in evidence and the research disposition. SbnG citrate supply, SbnD transport, iron loading, receptor recognition and regulation are contextual processes outside the seven-object native boundary.

Pathway type
staphyloferrin b biosynthesis
Organism scope

Read the source YAML

Pathway network

42 nodes · 72 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Pathway network: staphyloferrin B biosynthesis 42 nodes · 72 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. CHEBI:33384 — consumes → RHEA:59113; Evidence: RHEA:59113consumes CHEBI:30616 — consumes → RHEA:59113; Evidence: RHEA:59113consumes RHEA:59113 — produces → CHEBI:57524; Evidence: RHEA:59113produces RHEA:59113 — produces → CHEBI:456216; Evidence: RHEA:59113produces RHEA:59113 — produces → CHEBI:15378; Evidence: RHEA:59113produces CHEBI:57524 — consumes → RHEA:52385; Evidence: RHEA:52385consumes CHEBI:29985 — consumes → RHEA:52385; Evidence: RHEA:52385consumes RHEA:52385 — produces → CHEBI:134610; Evidence: RHEA:52385produces RHEA:52385 — produces → CHEBI:43474; Evidence: RHEA:52385produces RHEA:52385 — produces → CHEBI:15378; Evidence: RHEA:52385produces CHEBI:134610 — consumes → RHEA:51929; Evidence: RHEA:51929consumes CHEBI:57540 — consumes → RHEA:51929; Evidence: RHEA:51929consumes CHEBI:15377 — consumes → RHEA:51929; Evidence: RHEA:51929consumes RHEA:51929 — produces → CHEBI:57721; Evidence: RHEA:51929produces RHEA:51929 — produces → CHEBI:16810; Evidence: RHEA:51929produces RHEA:51929 — produces → CHEBI:57945; Evidence: RHEA:51929produces RHEA:51929 — produces → CHEBI:15378; Evidence: RHEA:51929produces CHEBI:57721 — consumes → RHEA:59125; Evidence: RHEA:59125consumes CHEBI:16947 — consumes → RHEA:59125; Evidence: RHEA:59125consumes CHEBI:30616 — consumes → RHEA:59125; Evidence: RHEA:59125consumes RHEA:59125 — produces → CHEBI:142969; Evidence: RHEA:59125, CHEBI:136993produces RHEA:59125 — produces → CHEBI:456215; Evidence: RHEA:59125produces RHEA:59125 — produces → CHEBI:33019; Evidence: RHEA:59125produces CHEBI:142969 — consumes → RHEA:59121; Evidence: RHEA:59121, CHEBI:136993consumes CHEBI:15378 — consumes → RHEA:59121; Evidence: RHEA:59121consumes RHEA:59121 — produces → CHEBI:142970; Evidence: RHEA:59121, CHEBI:136993produces RHEA:59121 — produces → CHEBI:16526; Evidence: RHEA:59121produces CHEBI:142970 — consumes → RHEA:59129; Evidence: RHEA:59129, CHEBI:136993consumes CHEBI:57721 — consumes → RHEA:59129; Evidence: RHEA:59129consumes CHEBI:30616 — consumes → RHEA:59129; Evidence: RHEA:59129consumes RHEA:59129 — produces → CHEBI:142971; Evidence: RHEA:59129, CHEBI:136993produces RHEA:59129 — produces → CHEBI:456215; Evidence: RHEA:59129produces RHEA:59129 — produces → CHEBI:33019; Evidence: RHEA:59129produces CHEBI:142971 — consumes → RHEA:59133; Evidence: RHEA:59133, CHEBI:136993consumes CHEBI:16810 — consumes → RHEA:59133; Evidence: RHEA:59133consumes CHEBI:30616 — consumes → RHEA:59133; Evidence: RHEA:59133consumes RHEA:59133 — produces → CHEBI:136993; Formal open-chain dianion output of the current Rhea net equation. The primary characterized cyclic hemiaminal is a distinct connectivity; no claim of a uniquely linear physiological species or exact identity is made.; Evidence: RHEA:59133, CHEBI:136993, DOI:10.1021/jacs.5b04557.s001, PubChem:122234488produces RHEA:59133 — produces → CHEBI:456215; Evidence: RHEA:59133produces RHEA:59133 — produces → CHEBI:33019; Evidence: RHEA:59133produces RHEA:59133 — produces → CHEBI:15378; Evidence: RHEA:59133produces RHEA:59113 — part_of → MetaCyc:PWY-8008; Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed.; Evidence: MetaCyc:PWY-8008, MetaCyc:RXN-19819, RHEA:59113part of RHEA:52385 — part_of → MetaCyc:PWY-8008; Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed. Native proton bookkeeping differs; current balanced Rhea coefficients are selected.; Evidence: MetaCyc:PWY-8008, MetaCyc:RXN-18395, RHEA:52385, MetaCyc:RXN-18395part of RHEA:51929 — part_of → MetaCyc:PWY-8008; Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed.; Evidence: MetaCyc:PWY-8008, MetaCyc:RXN-18393, RHEA:51929, MetaCyc:RXN-18393part of RHEA:59125 — part_of → MetaCyc:PWY-8008; Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed. Native proton bookkeeping differs; current balanced Rhea coefficients are selected.; Evidence: MetaCyc:PWY-8008, MetaCyc:RXN-19820, RHEA:59125part of RHEA:59121 — part_of → MetaCyc:PWY-8008; Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed.; Evidence: MetaCyc:PWY-8008, MetaCyc:RXN-19821, RHEA:59121part of RHEA:59129 — part_of → MetaCyc:PWY-8008; Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed. Native proton bookkeeping differs; current balanced Rhea coefficients are selected.; Evidence: MetaCyc:PWY-8008, MetaCyc:RXN-19822, RHEA:59129part of RHEA:59133 — part_of → MetaCyc:PWY-8008; Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed. The stored final product is linear; cyclic primary product characterization is retained as a molecular-form limitation.; Evidence: MetaCyc:PWY-8008, MetaCyc:RXN-19823, RHEA:59133part of NCBIProtein:BAF66340.1 — enables → RHEA:59113; Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.; Evidence: PMID:29483190, NCBIProtein:BAF66340.1, UniProtKB:Q2G1M5enables NCBIProtein:BAF66332.1 — enables → RHEA:52385; Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.; Evidence: DOI:10.14288/1.0228113, NCBIProtein:BAF66332.1, UniProtKB:Q2G1N3enables NCBIProtein:BAF66333.1 — enables → RHEA:51929; Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.; Evidence: DOI:10.14288/1.0228113, NCBIProtein:BAF66333.1, UniProtKB:Q2G1N2, DOI:10.14288/1.0228113enables NCBIProtein:BAF66336.1 — enables → RHEA:59125; Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.; Evidence: PMID:19775248, NCBIProtein:BAF66336.1, UniProtKB:Q2G1M9enables NCBIProtein:BAF66339.1 — enables → RHEA:59121; Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.; Evidence: PMID:19775248, NCBIProtein:BAF66339.1, UniProtKB:Q2G1M6enables NCBIProtein:BAF66337.1 — enables → RHEA:59129; Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.; Evidence: PMID:19775248, NCBIProtein:BAF66337.1, UniProtKB:Q2G1M8enables NCBIProtein:BAF66334.1 — enables → RHEA:59133; Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.; Evidence: PMID:19775248, NCBIProtein:BAF66334.1, UniProtKB:Q2G1N1enables UniProtKB:Q2G1M5 — part_of → MetaCyc:CPLX-9312; The named component and complex retain NCTC 8325 source identity; they are not renamed Newman entities.; Evidence: MetaCyc:CPLX-9312, MetaCyc:G1I0R-77-MONOMERpart of MetaCyc:CPLX-9312 — catalyzes → RHEA:59113; Curated NCTC 8325 assembly/activity assertion. SbnI oligomerization is not essential for kinase activity; SbnB solution dimer evidence is independently reported for Newman-derived protein.; Evidence: MetaCyc:CPLX-9312, UniProtKB:Q2G1M5catalyzes UniProtKB:Q2G1N2 — part_of → MetaCyc:CPLX-9313; The named component and complex retain NCTC 8325 source identity; they are not renamed Newman entities.; Evidence: MetaCyc:CPLX-9313, MetaCyc:G1I0R-70-MONOMERpart of MetaCyc:CPLX-9313 — catalyzes → RHEA:51929; Curated NCTC 8325 assembly/activity assertion. SbnI oligomerization is not essential for kinase activity; SbnB solution dimer evidence is independently reported for Newman-derived protein.; Evidence: MetaCyc:CPLX-9313, UniProtKB:Q2G1N2catalyzes NCBIProtein:BAF66332.1 — has_cofactor → CHEBI:597326; Evidence: PMID:26794841, UniProtKB:Q2G1N3has cofactor NCBIProtein:BAF66339.1 — has_cofactor → CHEBI:597326; Evidence: PMID:19775248, UniProtKB:Q2G1M6has cofactor MetaCyc:CPLX-9312 — has_cofactor → CHEBI:18420; Qualified native cofactor annotation. Newman ligase roles use exact sequence correspondence to the native NCTC 8325 gene product; the complete-system Mg omission experiment does not establish individual binding counts.; Evidence: MetaCyc:ENZRXN-26580has cofactor NCBIProtein:BAF66336.1 — has_cofactor → CHEBI:18420; Qualified native cofactor annotation. Newman ligase roles use exact sequence correspondence to the native NCTC 8325 gene product; the complete-system Mg omission experiment does not establish individual binding counts.; Evidence: MetaCyc:ENZRXN-26574, PMID:19775248has cofactor NCBIProtein:BAF66337.1 — has_cofactor → CHEBI:18420; Qualified native cofactor annotation. Newman ligase roles use exact sequence correspondence to the native NCTC 8325 gene product; the complete-system Mg omission experiment does not establish individual binding counts.; Evidence: MetaCyc:ENZRXN-26576, PMID:19775248has cofactor NCBIProtein:BAF66334.1 — has_cofactor → CHEBI:18420; Qualified native cofactor annotation. Newman ligase roles use exact sequence correspondence to the native NCTC 8325 gene product; the complete-system Mg omission experiment does not establish individual binding counts.; Evidence: MetaCyc:ENZRXN-26577, PMID:19775248has cofactor RHEA:59113 — provides_input_for → RHEA:52385; Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.; Evidence: RHEA:59113, RHEA:52385provides input for RHEA:52385 — provides_input_for → RHEA:51929; Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.; Evidence: RHEA:52385, RHEA:51929provides input for RHEA:51929 — provides_input_for → RHEA:59125; Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.; Evidence: RHEA:51929, RHEA:59125provides input for RHEA:59125 — provides_input_for → RHEA:59121; Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.; Evidence: RHEA:59125, RHEA:59121provides input for RHEA:59121 — provides_input_for → RHEA:59129; Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.; Evidence: RHEA:59121, RHEA:59129provides input for RHEA:51929 — provides_input_for → RHEA:59129; Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.; Evidence: RHEA:51929, RHEA:59129provides input for RHEA:59129 — provides_input_for → RHEA:59133; Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.; Evidence: RHEA:59129, RHEA:59133provides input for RHEA:51929 — provides_input_for → RHEA:59133; Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.; Evidence: RHEA:51929, RHEA:59133provides input for N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate(2-); CHEBI:134610; Declared type: small_molecule small molecule N-[(2S)-2-amino-2- carboxyethyl]-L-glutamate(2- ) staphyloferrin B(2-); CHEBI:136993; Declared type: small_molecule small molecule staphyloferrin B(2-) 2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate(2-); CHEBI:142969; Declared type: small_molecule small molecule 2-[(L-alanin-3- ylcarbamoyl)methyl]-2- hydroxybutanedioate(2-) 2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate; CHEBI:142970; Declared type: small_molecule small molecule 2-[(2- aminoethylcarbamoyl)methyl]- 2-hydroxybutanedioate 2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate; CHEBI:142971; Declared type: small_molecule small molecule 2-[(L-alanin-3- ylcarbamoyl)methyl]-3-(2- aminoethylcarbamoyl)-2- hydroxypropanoate water; CHEBI:15377; Declared type: small_molecule small molecule water hydron; CHEBI:15378; Declared type: small_molecule small molecule hydron carbon dioxide; CHEBI:16526; Declared type: small_molecule small molecule carbon dioxide 2-oxoglutarate(2-); CHEBI:16810; Declared type: small_molecule small molecule 2-oxoglutarate(2-) citrate(3-); CHEBI:16947; Declared type: small_molecule small molecule citrate(3-) magnesium(2+); CHEBI:18420; Declared type: cofactor cofactor magnesium(2+) L-glutamate(1-); CHEBI:29985; Declared type: small_molecule small molecule L-glutamate(1-) ATP(4-); CHEBI:30616; Declared type: small_molecule small molecule ATP(4-) diphosphate(3-); CHEBI:33019; Declared type: small_molecule small molecule diphosphate(3-) L-serine zwitterion; CHEBI:33384; Declared type: small_molecule small molecule L-serine zwitterion hydrogenphosphate; CHEBI:43474; Declared type: small_molecule small molecule hydrogenphosphate adenosine 5'-monophosphate(2-); CHEBI:456215; Declared type: small_molecule small molecule adenosine 5'-monophosphate(2-) ADP(3-); CHEBI:456216; Declared type: small_molecule small molecule ADP(3-) O-phosphonato-L-serine(2-); CHEBI:57524; Declared type: small_molecule small molecule O-phosphonato-L-serine(2-) NAD(1-); CHEBI:57540; Declared type: small_molecule small molecule NAD(1-) 3-amino-L-alanine zwitterion; CHEBI:57721; Declared type: small_molecule small molecule 3-amino-L-alanine zwitterion NADH(2-); CHEBI:57945; Declared type: small_molecule small molecule NADH(2-) pyridoxal 5'-phosphate(2-); CHEBI:597326; Declared type: cofactor cofactor pyridoxal 5'-phosphate(2-) NWMN_0068; NCBIProtein:BAF66340.1; Declared type: protein protein NWMN_0068 NWMN_0060; NCBIProtein:BAF66332.1; Declared type: protein protein NWMN_0060 NWMN_0061; NCBIProtein:BAF66333.1; Declared type: protein protein NWMN_0061 NWMN_0064; NCBIProtein:BAF66336.1; Declared type: protein protein NWMN_0064 NWMN_0067; NCBIProtein:BAF66339.1; Declared type: protein protein NWMN_0067 NWMN_0065; NCBIProtein:BAF66337.1; Declared type: protein protein NWMN_0065 NWMN_0062; NCBIProtein:BAF66334.1; Declared type: protein protein NWMN_0062 ATP-dependent L-serine kinase SbnI; UniProtKB:Q2G1M5; Declared type: protein protein ATP-dependent L-serine kinase SbnI N-((2S)-2-amino-2-carboxyethyl)-L-glutamate dehydrogenase; UniProtKB:Q2G1N2; Declared type: protein protein N-((2S)-2-amino-2- carboxyethyl)-L-glutamate dehydrogenase L-serine kinase; MetaCyc:CPLX-9312; Declared type: complex complex L-serine kinase N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate dehydrogenase; MetaCyc:CPLX-9313; Declared type: complex complex N-[(2S)-2-amino-2- carboxyethyl]-L-glutamate dehydrogenase SbnI free L-serine phosphorylation; RHEA:59113; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity SbnI free L-serine phosphorylation SbnA ACEGA formation; RHEA:52385; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity SbnA ACEGA formation SbnB oxidative ACEGA hydrolysis; RHEA:51929; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity SbnB oxidative ACEGA hydrolysis SbnE citryl-DAP ligation; RHEA:59125; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity SbnE citryl-DAP ligation SbnH citryl-DAP decarboxylation; RHEA:59121; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity SbnH citryl-DAP decarboxylation SbnF second DAP ligation; RHEA:59129; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity SbnF second DAP ligation SbnC terminal ligation under the formal open-chain product convention; RHEA:59133; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity SbnC terminal ligation under the formal open-chain product convention staphyloferrin B biosynthesis; MetaCyc:PWY-8008; Declared type: pathway pathway staphyloferrin B biosynthesis

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Staphylococcus aureus subsp. aureus str. Newman
NCBITaxon:426430		taxon
Staphylococcus aureus subsp. aureus NCTC 8325
NCBITaxon:93061		taxon
N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate(2-)
CHEBI:134610		small molecule
staphyloferrin B(2-)
CHEBI:136993		small molecule
2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate(2-)
CHEBI:142969		small molecule
2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate
CHEBI:142970		small molecule
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate
CHEBI:142971		small molecule
water
CHEBI:15377		small molecule
hydron
CHEBI:15378		small molecule
carbon dioxide
CHEBI:16526		small molecule
2-oxoglutarate(2-)
CHEBI:16810		small molecule
citrate(3-)
CHEBI:16947		small molecule
magnesium(2+)
CHEBI:18420		cofactor
L-glutamate(1-)
CHEBI:29985		small molecule
ATP(4-)
CHEBI:30616		small molecule
diphosphate(3-)
CHEBI:33019		small molecule
L-serine zwitterion
CHEBI:33384		small molecule
hydrogenphosphate
CHEBI:43474		small molecule
adenosine 5'-monophosphate(2-)
CHEBI:456215		small molecule
ADP(3-)
CHEBI:456216		small molecule
O-phosphonato-L-serine(2-)
CHEBI:57524		small molecule
NAD(1-)
CHEBI:57540		small molecule
3-amino-L-alanine zwitterion
CHEBI:57721		small molecule
NADH(2-)
CHEBI:57945		small molecule
pyridoxal 5'-phosphate(2-)
CHEBI:597326		cofactor
NWMN_0068
NCBIProtein:BAF66340.1		protein
NWMN_0060
NCBIProtein:BAF66332.1		protein
NWMN_0061
NCBIProtein:BAF66333.1		protein
NWMN_0064
NCBIProtein:BAF66336.1		protein
NWMN_0067
NCBIProtein:BAF66339.1		protein
NWMN_0065
NCBIProtein:BAF66337.1		protein
NWMN_0062
NCBIProtein:BAF66334.1		protein
ATP-dependent L-serine kinase SbnI
UniProtKB:Q2G1M5		protein
N-((2S)-2-amino-2-carboxyethyl)-L-glutamate dehydrogenase
UniProtKB:Q2G1N2		protein
L-serine kinase
MetaCyc:CPLX-9312		complex
N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate dehydrogenase
MetaCyc:CPLX-9313		complex
SbnI free L-serine phosphorylation
RHEA:59113		molecular activityleft to right
SbnA ACEGA formation
RHEA:52385		molecular activityleft to right
SbnB oxidative ACEGA hydrolysis
RHEA:51929		molecular activityleft to right
SbnE citryl-DAP ligation
RHEA:59125		molecular activityleft to right
SbnH citryl-DAP decarboxylation
RHEA:59121		molecular activityleft to right
SbnF second DAP ligation
RHEA:59129		molecular activityleft to right
SbnC terminal ligation under the formal open-chain product convention
RHEA:59133		molecular activityleft to right

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
L-serine zwitterion
CHEBI:33384		consumes

SbnI free L-serine phosphorylation
RHEA:59113
  • RHEA:59113

    Source assertion: The selected Rhea direction assigns CHEBI:33384 to substrates with coefficient 1.

    Source locationrh:59113/rh:substrates -> http://rdf.rhea-db.org/59112_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59112_compound_4901; rh:compound -> http://rdf.rhea-db.org/Compound_4901; rh:chebi
ATP(4-)
CHEBI:30616		consumes

SbnI free L-serine phosphorylation
RHEA:59113
  • RHEA:59113

    Source assertion: The selected Rhea direction assigns CHEBI:30616 to substrates with coefficient 1.

    Source locationrh:59113/rh:substrates -> http://rdf.rhea-db.org/59112_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59112_compound_6372; rh:compound -> http://rdf.rhea-db.org/Compound_6372; rh:chebi
SbnI free L-serine phosphorylation
RHEA:59113		produces

O-phosphonato-L-serine(2-)
CHEBI:57524
  • RHEA:59113

    Source assertion: The selected Rhea direction assigns CHEBI:57524 to products with coefficient 1.

    Source locationrh:59113/rh:products -> http://rdf.rhea-db.org/59112_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59112_compound_1236; rh:compound -> http://rdf.rhea-db.org/Compound_1236; rh:chebi
SbnI free L-serine phosphorylation
RHEA:59113		produces

ADP(3-)
CHEBI:456216
  • RHEA:59113

    Source assertion: The selected Rhea direction assigns CHEBI:456216 to products with coefficient 1.

    Source locationrh:59113/rh:products -> http://rdf.rhea-db.org/59112_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59112_compound_2710; rh:compound -> http://rdf.rhea-db.org/Compound_2710; rh:chebi
SbnI free L-serine phosphorylation
RHEA:59113		produces

hydron
CHEBI:15378
  • RHEA:59113

    Source assertion: The selected Rhea direction assigns CHEBI:15378 to products with coefficient 1.

    Source locationrh:59113/rh:products -> http://rdf.rhea-db.org/59112_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59112_compound_3249; rh:compound -> http://rdf.rhea-db.org/Compound_3249; rh:chebi
O-phosphonato-L-serine(2-)
CHEBI:57524		consumes

SbnA ACEGA formation
RHEA:52385
  • RHEA:52385

    Source assertion: The selected Rhea direction assigns CHEBI:57524 to substrates with coefficient 1.

    Source locationrh:52385/rh:substrates -> http://rdf.rhea-db.org/52384_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_52384_compound_1236; rh:compound -> http://rdf.rhea-db.org/Compound_1236; rh:chebi
L-glutamate(1-)
CHEBI:29985		consumes

SbnA ACEGA formation
RHEA:52385
  • RHEA:52385

    Source assertion: The selected Rhea direction assigns CHEBI:29985 to substrates with coefficient 1.

    Source locationrh:52385/rh:substrates -> http://rdf.rhea-db.org/52384_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_52384_compound_2079; rh:compound -> http://rdf.rhea-db.org/Compound_2079; rh:chebi
SbnA ACEGA formation
RHEA:52385		produces

N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate(2-)
CHEBI:134610
  • RHEA:52385

    Source assertion: The selected Rhea direction assigns CHEBI:134610 to products with coefficient 1.

    Source locationrh:52385/rh:products -> http://rdf.rhea-db.org/52384_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_52384_compound_13099; rh:compound -> http://rdf.rhea-db.org/Compound_13099; rh:chebi
SbnA ACEGA formation
RHEA:52385		produces

hydrogenphosphate
CHEBI:43474
  • RHEA:52385

    Source assertion: The selected Rhea direction assigns CHEBI:43474 to products with coefficient 1.

    Source locationrh:52385/rh:products -> http://rdf.rhea-db.org/52384_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_52384_compound_5518; rh:compound -> http://rdf.rhea-db.org/Compound_5518; rh:chebi
SbnA ACEGA formation
RHEA:52385		produces

hydron
CHEBI:15378
  • RHEA:52385

    Source assertion: The selected Rhea direction assigns CHEBI:15378 to products with coefficient 1.

    Source locationrh:52385/rh:products -> http://rdf.rhea-db.org/52384_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_52384_compound_3249; rh:compound -> http://rdf.rhea-db.org/Compound_3249; rh:chebi
N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate(2-)
CHEBI:134610		consumes

SbnB oxidative ACEGA hydrolysis
RHEA:51929
  • RHEA:51929

    Source assertion: The selected Rhea direction assigns CHEBI:134610 to substrates with coefficient 1.

    Source locationrh:51929/rh:substrates -> http://rdf.rhea-db.org/51928_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_13099; rh:compound -> http://rdf.rhea-db.org/Compound_13099; rh:chebi
NAD(1-)
CHEBI:57540		consumes

SbnB oxidative ACEGA hydrolysis
RHEA:51929
  • RHEA:51929

    Source assertion: The selected Rhea direction assigns CHEBI:57540 to substrates with coefficient 1.

    Source locationrh:51929/rh:substrates -> http://rdf.rhea-db.org/51928_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_3484; rh:compound -> http://rdf.rhea-db.org/Compound_3484; rh:chebi
water
CHEBI:15377		consumes

SbnB oxidative ACEGA hydrolysis
RHEA:51929
  • RHEA:51929

    Source assertion: The selected Rhea direction assigns CHEBI:15377 to substrates with coefficient 1.

    Source locationrh:51929/rh:substrates -> http://rdf.rhea-db.org/51928_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_1283; rh:compound -> http://rdf.rhea-db.org/Compound_1283; rh:chebi
SbnB oxidative ACEGA hydrolysis
RHEA:51929		produces

3-amino-L-alanine zwitterion
CHEBI:57721
  • RHEA:51929

    Source assertion: The selected Rhea direction assigns CHEBI:57721 to products with coefficient 1.

    Source locationrh:51929/rh:products -> http://rdf.rhea-db.org/51928_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_1905; rh:compound -> http://rdf.rhea-db.org/Compound_1905; rh:chebi
SbnB oxidative ACEGA hydrolysis
RHEA:51929		produces

2-oxoglutarate(2-)
CHEBI:16810
  • RHEA:51929

    Source assertion: The selected Rhea direction assigns CHEBI:16810 to products with coefficient 1.

    Source locationrh:51929/rh:products -> http://rdf.rhea-db.org/51928_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_2975; rh:compound -> http://rdf.rhea-db.org/Compound_2975; rh:chebi
SbnB oxidative ACEGA hydrolysis
RHEA:51929		produces

NADH(2-)
CHEBI:57945
  • RHEA:51929

    Source assertion: The selected Rhea direction assigns CHEBI:57945 to products with coefficient 1.

    Source locationrh:51929/rh:products -> http://rdf.rhea-db.org/51928_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_1650; rh:compound -> http://rdf.rhea-db.org/Compound_1650; rh:chebi
SbnB oxidative ACEGA hydrolysis
RHEA:51929		produces

hydron
CHEBI:15378
  • RHEA:51929

    Source assertion: The selected Rhea direction assigns CHEBI:15378 to products with coefficient 1.

    Source locationrh:51929/rh:products -> http://rdf.rhea-db.org/51928_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_3249; rh:compound -> http://rdf.rhea-db.org/Compound_3249; rh:chebi
3-amino-L-alanine zwitterion
CHEBI:57721		consumes

SbnE citryl-DAP ligation
RHEA:59125
  • RHEA:59125

    Source assertion: The selected Rhea direction assigns CHEBI:57721 to substrates with coefficient 1.

    Source locationrh:59125/rh:substrates -> http://rdf.rhea-db.org/59124_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59124_compound_1905; rh:compound -> http://rdf.rhea-db.org/Compound_1905; rh:chebi
citrate(3-)
CHEBI:16947		consumes

SbnE citryl-DAP ligation
RHEA:59125
  • RHEA:59125

    Source assertion: The selected Rhea direction assigns CHEBI:16947 to substrates with coefficient 1.

    Source locationrh:59125/rh:substrates -> http://rdf.rhea-db.org/59124_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59124_compound_5244; rh:compound -> http://rdf.rhea-db.org/Compound_5244; rh:chebi
ATP(4-)
CHEBI:30616		consumes

SbnE citryl-DAP ligation
RHEA:59125
  • RHEA:59125

    Source assertion: The selected Rhea direction assigns CHEBI:30616 to substrates with coefficient 1.

    Source locationrh:59125/rh:substrates -> http://rdf.rhea-db.org/59124_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59124_compound_6372; rh:compound -> http://rdf.rhea-db.org/Compound_6372; rh:chebi
SbnE citryl-DAP ligation
RHEA:59125		produces

2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate(2-)
CHEBI:142969
  • RHEA:59125

    Source assertion: The selected Rhea direction assigns CHEBI:142969 to products with coefficient 1.

    Source locationrh:59125/rh:products -> http://rdf.rhea-db.org/59124_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59124_compound_15297; rh:compound -> http://rdf.rhea-db.org/Compound_15297; rh:chebi
  • CHEBI:136993

    Source assertion: The selected ChEBI structure specifies the citryl center; this is a current chemical-authority convention, not a direct stereochemical assay on this Newman intermediate.

    Source locationSemantic SQL CHEBI:142969/chemrof:inchi_string; rdfs:label
SbnE citryl-DAP ligation
RHEA:59125		produces

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • RHEA:59125

    Source assertion: The selected Rhea direction assigns CHEBI:456215 to products with coefficient 1.

    Source locationrh:59125/rh:products -> http://rdf.rhea-db.org/59124_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59124_compound_2590; rh:compound -> http://rdf.rhea-db.org/Compound_2590; rh:chebi
SbnE citryl-DAP ligation
RHEA:59125		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:59125

    Source assertion: The selected Rhea direction assigns CHEBI:33019 to products with coefficient 1.

    Source locationrh:59125/rh:products -> http://rdf.rhea-db.org/59124_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59124_compound_2106; rh:compound -> http://rdf.rhea-db.org/Compound_2106; rh:chebi
2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate(2-)
CHEBI:142969		consumes

SbnH citryl-DAP decarboxylation
RHEA:59121
  • RHEA:59121

    Source assertion: The selected Rhea direction assigns CHEBI:142969 to substrates with coefficient 1.

    Source locationrh:59121/rh:substrates -> http://rdf.rhea-db.org/59120_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59120_compound_15297; rh:compound -> http://rdf.rhea-db.org/Compound_15297; rh:chebi
  • CHEBI:136993

    Source assertion: The selected ChEBI structure specifies the citryl center; this is a current chemical-authority convention, not a direct stereochemical assay on this Newman intermediate.

    Source locationSemantic SQL CHEBI:142969/chemrof:inchi_string; rdfs:label
hydron
CHEBI:15378		consumes

SbnH citryl-DAP decarboxylation
RHEA:59121
  • RHEA:59121

    Source assertion: The selected Rhea direction assigns CHEBI:15378 to substrates with coefficient 1.

    Source locationrh:59121/rh:substrates -> http://rdf.rhea-db.org/59120_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59120_compound_3249; rh:compound -> http://rdf.rhea-db.org/Compound_3249; rh:chebi
SbnH citryl-DAP decarboxylation
RHEA:59121		produces

2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate
CHEBI:142970
  • RHEA:59121

    Source assertion: The selected Rhea direction assigns CHEBI:142970 to products with coefficient 1.

    Source locationrh:59121/rh:products -> http://rdf.rhea-db.org/59120_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59120_compound_15295; rh:compound -> http://rdf.rhea-db.org/Compound_15295; rh:chebi
  • CHEBI:136993

    Source assertion: The selected ChEBI structure specifies the citryl center; this is a current chemical-authority convention, not a direct stereochemical assay on this Newman intermediate.

    Source locationSemantic SQL CHEBI:142970/chemrof:inchi_string; rdfs:label
SbnH citryl-DAP decarboxylation
RHEA:59121		produces

carbon dioxide
CHEBI:16526
  • RHEA:59121

    Source assertion: The selected Rhea direction assigns CHEBI:16526 to products with coefficient 1.

    Source locationrh:59121/rh:products -> http://rdf.rhea-db.org/59120_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59120_compound_4391; rh:compound -> http://rdf.rhea-db.org/Compound_4391; rh:chebi
2-[(2-aminoethylcarbamoyl)methyl]-2-hydroxybutanedioate
CHEBI:142970		consumes

SbnF second DAP ligation
RHEA:59129
  • RHEA:59129

    Source assertion: The selected Rhea direction assigns CHEBI:142970 to substrates with coefficient 1.

    Source locationrh:59129/rh:substrates -> http://rdf.rhea-db.org/59128_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59128_compound_15295; rh:compound -> http://rdf.rhea-db.org/Compound_15295; rh:chebi
  • CHEBI:136993

    Source assertion: The selected ChEBI structure specifies the citryl center; this is a current chemical-authority convention, not a direct stereochemical assay on this Newman intermediate.

    Source locationSemantic SQL CHEBI:142970/chemrof:inchi_string; rdfs:label
3-amino-L-alanine zwitterion
CHEBI:57721		consumes

SbnF second DAP ligation
RHEA:59129
  • RHEA:59129

    Source assertion: The selected Rhea direction assigns CHEBI:57721 to substrates with coefficient 1.

    Source locationrh:59129/rh:substrates -> http://rdf.rhea-db.org/59128_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59128_compound_1905; rh:compound -> http://rdf.rhea-db.org/Compound_1905; rh:chebi
ATP(4-)
CHEBI:30616		consumes

SbnF second DAP ligation
RHEA:59129
  • RHEA:59129

    Source assertion: The selected Rhea direction assigns CHEBI:30616 to substrates with coefficient 1.

    Source locationrh:59129/rh:substrates -> http://rdf.rhea-db.org/59128_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59128_compound_6372; rh:compound -> http://rdf.rhea-db.org/Compound_6372; rh:chebi
SbnF second DAP ligation
RHEA:59129		produces

2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate
CHEBI:142971
  • RHEA:59129

    Source assertion: The selected Rhea direction assigns CHEBI:142971 to products with coefficient 1.

    Source locationrh:59129/rh:products -> http://rdf.rhea-db.org/59128_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59128_compound_15296; rh:compound -> http://rdf.rhea-db.org/Compound_15296; rh:chebi
  • CHEBI:136993

    Source assertion: The selected ChEBI structure specifies the citryl center; this is a current chemical-authority convention, not a direct stereochemical assay on this Newman intermediate.

    Source locationSemantic SQL CHEBI:142971/chemrof:inchi_string; rdfs:label
SbnF second DAP ligation
RHEA:59129		produces

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • RHEA:59129

    Source assertion: The selected Rhea direction assigns CHEBI:456215 to products with coefficient 1.

    Source locationrh:59129/rh:products -> http://rdf.rhea-db.org/59128_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59128_compound_2590; rh:compound -> http://rdf.rhea-db.org/Compound_2590; rh:chebi
SbnF second DAP ligation
RHEA:59129		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:59129

    Source assertion: The selected Rhea direction assigns CHEBI:33019 to products with coefficient 1.

    Source locationrh:59129/rh:products -> http://rdf.rhea-db.org/59128_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59128_compound_2106; rh:compound -> http://rdf.rhea-db.org/Compound_2106; rh:chebi
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate
CHEBI:142971		consumes

SbnC terminal ligation under the formal open-chain product convention
RHEA:59133
  • RHEA:59133

    Source assertion: The selected Rhea direction assigns CHEBI:142971 to substrates with coefficient 1.

    Source locationrh:59133/rh:substrates -> http://rdf.rhea-db.org/59132_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59132_compound_15296; rh:compound -> http://rdf.rhea-db.org/Compound_15296; rh:chebi
  • CHEBI:136993

    Source assertion: The selected ChEBI structure specifies the citryl center; this is a current chemical-authority convention, not a direct stereochemical assay on this Newman intermediate.

    Source locationSemantic SQL CHEBI:142971/chemrof:inchi_string; rdfs:label
2-oxoglutarate(2-)
CHEBI:16810		consumes

SbnC terminal ligation under the formal open-chain product convention
RHEA:59133
  • RHEA:59133

    Source assertion: The selected Rhea direction assigns CHEBI:16810 to substrates with coefficient 1.

    Source locationrh:59133/rh:substrates -> http://rdf.rhea-db.org/59132_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59132_compound_2975; rh:compound -> http://rdf.rhea-db.org/Compound_2975; rh:chebi
ATP(4-)
CHEBI:30616		consumes

SbnC terminal ligation under the formal open-chain product convention
RHEA:59133
  • RHEA:59133

    Source assertion: The selected Rhea direction assigns CHEBI:30616 to substrates with coefficient 1.

    Source locationrh:59133/rh:substrates -> http://rdf.rhea-db.org/59132_L; rh:contains1 -> http://rdf.rhea-db.org/Participant_59132_compound_6372; rh:compound -> http://rdf.rhea-db.org/Compound_6372; rh:chebi
SbnC terminal ligation under the formal open-chain product convention
RHEA:59133		produces

Formal open-chain dianion output of the current Rhea net equation. The primary characterized cyclic hemiaminal is a distinct connectivity; no claim of a uniquely linear physiological species or exact identity is made.

staphyloferrin B(2-)
CHEBI:136993
  • RHEA:59133

    Source assertion: The selected Rhea direction assigns CHEBI:136993 to products with coefficient 1.

    Source locationrh:59133/rh:products -> http://rdf.rhea-db.org/59132_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59132_compound_15367; rh:compound -> http://rdf.rhea-db.org/Compound_15367; rh:chebi
  • CHEBI:136993

    Source assertion: CHEBI:136993 has an open-chain alpha-ketoamide terminus and specified S-citrate center; it is not the cyclic hemiaminal neutral structure.

    Source locationSemantic SQL CHEBI:136993/chemrof:inchi_string; chemrof:charge
  • DOI:10.1021/jacs.5b04557.s001

    Source assertion: The original SB structure is cyclic; S7 describes a 1:1 epimer mixture arising from equilibrating lactamization. This limits the interpretation of the formal linear output, rather than demonstrating a linear-only physiological product.

    Source locationS7, SB1 product-description paragraph; S38 TableS3; S39 TableS4; S42 FigureS2
  • PubChem:122234488

    Source assertion: CID 122234488 independently defines a neutral cyclic hemiaminal with S amino-acid/S citrate centers and an unspecified additional center, a distinct structure from the formal linear dianion.

    Source location/PropertyTable/Properties[0]/InChI, InChIKey, IUPACName, Charge
SbnC terminal ligation under the formal open-chain product convention
RHEA:59133		produces

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • RHEA:59133

    Source assertion: The selected Rhea direction assigns CHEBI:456215 to products with coefficient 1.

    Source locationrh:59133/rh:products -> http://rdf.rhea-db.org/59132_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59132_compound_2590; rh:compound -> http://rdf.rhea-db.org/Compound_2590; rh:chebi
SbnC terminal ligation under the formal open-chain product convention
RHEA:59133		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:59133

    Source assertion: The selected Rhea direction assigns CHEBI:33019 to products with coefficient 1.

    Source locationrh:59133/rh:products -> http://rdf.rhea-db.org/59132_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59132_compound_2106; rh:compound -> http://rdf.rhea-db.org/Compound_2106; rh:chebi
SbnC terminal ligation under the formal open-chain product convention
RHEA:59133		produces

hydron
CHEBI:15378
  • RHEA:59133

    Source assertion: The selected Rhea direction assigns CHEBI:15378 to products with coefficient 1.

    Source locationrh:59133/rh:products -> http://rdf.rhea-db.org/59132_R; rh:contains1 -> http://rdf.rhea-db.org/Participant_59132_compound_3249; rh:compound -> http://rdf.rhea-db.org/Compound_3249; rh:chebi
SbnI free L-serine phosphorylation
RHEA:59113		part_of

Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed.

MetaCyc:PWY-8008
  • MetaCyc:PWY-8008

    Source assertion: PWY-8008 includes native reaction RXN-19819 within its seven-reaction biosynthetic definition.

    Source locationPathway[@frameid="PWY-8008"]/reaction-list/Reaction[@frameid="RXN-19819"]
  • MetaCyc:RXN-19819

    Source assertion: The native reaction is physiologically left-to-right. Its stored molecular forms and coefficients are retained separately from the selected current Rhea equation.

    Source locationReaction/reaction-direction; left; right
  • RHEA:59113

    Source assertion: The original Rhea master 59112 explicitly lists directed reaction 59113; its selected substrates are 59112_L and products 59112_R.

    Source locationrh:59112/rh:directionalReaction -> rh:59113; rh:59113/rh:substrates; rh:products
SbnA ACEGA formation
RHEA:52385		part_of

Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed. Native proton bookkeeping differs; current balanced Rhea coefficients are selected.

MetaCyc:PWY-8008
  • MetaCyc:PWY-8008

    Source assertion: PWY-8008 includes native reaction RXN-18395 within its seven-reaction biosynthetic definition.

    Source locationPathway[@frameid="PWY-8008"]/reaction-list/Reaction[@frameid="RXN-18395"]
  • MetaCyc:RXN-18395

    Source assertion: The native reaction is physiologically left-to-right. Its stored molecular forms and coefficients are retained separately from the selected current Rhea equation.

    Source locationReaction/reaction-direction; left; right
  • RHEA:52385

    Source assertion: The original Rhea master 52384 explicitly lists directed reaction 52385; its selected substrates are 52384_L and products 52384_R.

    Source locationrh:52384/rh:directionalReaction -> rh:52385; rh:52385/rh:substrates; rh:products
  • MetaCyc:RXN-18395

    Source assertion: The native RHEA dblink explicitly supplies the selected directed identifier.

    Source locationReaction/dblink[dblink-db="RHEA"]/dblink-oid
SbnB oxidative ACEGA hydrolysis
RHEA:51929		part_of

Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed.

MetaCyc:PWY-8008
  • MetaCyc:PWY-8008

    Source assertion: PWY-8008 includes native reaction RXN-18393 within its seven-reaction biosynthetic definition.

    Source locationPathway[@frameid="PWY-8008"]/reaction-list/Reaction[@frameid="RXN-18393"]
  • MetaCyc:RXN-18393

    Source assertion: The native reaction is physiologically left-to-right. Its stored molecular forms and coefficients are retained separately from the selected current Rhea equation.

    Source locationReaction/reaction-direction; left; right
  • RHEA:51929

    Source assertion: The original Rhea master 51928 explicitly lists directed reaction 51929; its selected substrates are 51928_L and products 51928_R.

    Source locationrh:51928/rh:directionalReaction -> rh:51929; rh:51929/rh:substrates; rh:products
  • MetaCyc:RXN-18393

    Source assertion: The native RHEA dblink explicitly supplies the selected directed identifier.

    Source locationReaction/dblink[dblink-db="RHEA"]/dblink-oid
SbnE citryl-DAP ligation
RHEA:59125		part_of

Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed. Native proton bookkeeping differs; current balanced Rhea coefficients are selected.

MetaCyc:PWY-8008
  • MetaCyc:PWY-8008

    Source assertion: PWY-8008 includes native reaction RXN-19820 within its seven-reaction biosynthetic definition.

    Source locationPathway[@frameid="PWY-8008"]/reaction-list/Reaction[@frameid="RXN-19820"]
  • MetaCyc:RXN-19820

    Source assertion: The native reaction is physiologically left-to-right. Its stored molecular forms and coefficients are retained separately from the selected current Rhea equation.

    Source locationReaction/reaction-direction; left; right
  • RHEA:59125

    Source assertion: The original Rhea master 59124 explicitly lists directed reaction 59125; its selected substrates are 59124_L and products 59124_R.

    Source locationrh:59124/rh:directionalReaction -> rh:59125; rh:59125/rh:substrates; rh:products
SbnH citryl-DAP decarboxylation
RHEA:59121		part_of

Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed.

MetaCyc:PWY-8008
  • MetaCyc:PWY-8008

    Source assertion: PWY-8008 includes native reaction RXN-19821 within its seven-reaction biosynthetic definition.

    Source locationPathway[@frameid="PWY-8008"]/reaction-list/Reaction[@frameid="RXN-19821"]
  • MetaCyc:RXN-19821

    Source assertion: The native reaction is physiologically left-to-right. Its stored molecular forms and coefficients are retained separately from the selected current Rhea equation.

    Source locationReaction/reaction-direction; left; right
  • RHEA:59121

    Source assertion: The original Rhea master 59120 explicitly lists directed reaction 59121; its selected substrates are 59120_L and products 59120_R.

    Source locationrh:59120/rh:directionalReaction -> rh:59121; rh:59121/rh:substrates; rh:products
SbnF second DAP ligation
RHEA:59129		part_of

Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed. Native proton bookkeeping differs; current balanced Rhea coefficients are selected.

MetaCyc:PWY-8008
  • MetaCyc:PWY-8008

    Source assertion: PWY-8008 includes native reaction RXN-19822 within its seven-reaction biosynthetic definition.

    Source locationPathway[@frameid="PWY-8008"]/reaction-list/Reaction[@frameid="RXN-19822"]
  • MetaCyc:RXN-19822

    Source assertion: The native reaction is physiologically left-to-right. Its stored molecular forms and coefficients are retained separately from the selected current Rhea equation.

    Source locationReaction/reaction-direction; left; right
  • RHEA:59129

    Source assertion: The original Rhea master 59128 explicitly lists directed reaction 59129; its selected substrates are 59128_L and products 59128_R.

    Source locationrh:59128/rh:directionalReaction -> rh:59129; rh:59129/rh:substrates; rh:products
SbnC terminal ligation under the formal open-chain product convention
RHEA:59133		part_of

Native and current equations are compared by explicit chemistry; no unasserted native Rhea cross-reference is claimed. The stored final product is linear; cyclic primary product characterization is retained as a molecular-form limitation.

MetaCyc:PWY-8008
  • MetaCyc:PWY-8008

    Source assertion: PWY-8008 includes native reaction RXN-19823 within its seven-reaction biosynthetic definition.

    Source locationPathway[@frameid="PWY-8008"]/reaction-list/Reaction[@frameid="RXN-19823"]
  • MetaCyc:RXN-19823

    Source assertion: The native reaction is physiologically left-to-right. Its stored molecular forms and coefficients are retained separately from the selected current Rhea equation.

    Source locationReaction/reaction-direction; left; right
  • RHEA:59133

    Source assertion: The original Rhea master 59132 explicitly lists directed reaction 59133; its selected substrates are 59132_L and products 59132_R.

    Source locationrh:59132/rh:directionalReaction -> rh:59133; rh:59133/rh:substrates; rh:products
NWMN_0068
NCBIProtein:BAF66340.1		enables

Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.

SbnI free L-serine phosphorylation
RHEA:59113
  • PMID:29483190

    Source assertion: Purified Newman-derived SbnI phosphorylates free L-serine to OPS. Full-length protein is predominantly dimeric; a truncated monomer remains catalytically active.

    Source locationResults, sec4 (dimer) and sec5 (free-serine kinase); Experimental procedures, sec11 (cloning)
  • NCBIProtein:BAF66340.1

    Source assertion: The original GenPept BAF66340.1 identifies locus NWMN_0068 in Newman, taxon 426430, with its native sequence.

    Source locationGBSet/GBSeq[GBSeq_accession-version="BAF66340.1"]/GBSeq_feature-table; GBSeq_sequence
  • UniProtKB:Q2G1M5

    Source assertion: The NCTC 8325 entry assigns RHEA:59112 and RHEA:59113 with ECO:0000269. Its original sequence is identical to the selected Newman native protein; experimental constructs remain distinct.

    Source location/results/0/comments/1/reaction; physiologicalReactions; /results/0/sequence/value
NWMN_0060
NCBIProtein:BAF66332.1		enables

Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.

SbnA ACEGA formation
RHEA:52385
  • DOI:10.14288/1.0228113

    Source assertion: Newman-derived SbnA forms ACEGA from OPS and glutamate; full-length coding-region origin and the measured precursor reaction are reported.

    Source locationMethods2.3.2 printed37/PDF59; Results4.2.1 printed89-92/PDF111-114
  • NCBIProtein:BAF66332.1

    Source assertion: The original GenPept BAF66332.1 identifies locus NWMN_0060 in Newman, taxon 426430, with its native sequence.

    Source locationGBSet/GBSeq[GBSeq_accession-version="BAF66332.1"]/GBSeq_feature-table; GBSeq_sequence
  • UniProtKB:Q2G1N3

    Source assertion: The NCTC 8325 entry assigns RHEA:52384 and RHEA:52385 with ECO:0000269. Its original sequence is identical to the selected Newman native protein; experimental constructs remain distinct.

    Source location/results/3/comments/1/reaction; physiologicalReactions; /results/3/sequence/value
NWMN_0061
NCBIProtein:BAF66333.1		enables

Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.

SbnB oxidative ACEGA hydrolysis
RHEA:51929
  • DOI:10.14288/1.0228113

    Source assertion: Newman-derived SbnB converts ACEGA and NAD to DAP, 2-oxoglutarate and NADH. The reverse reaction is also demonstrated; the chosen direction is biosynthetic flux.

    Source locationMethods2.3.3 printed38/PDF60; Results4.2.2 printed93-94/PDF115-116; Figure4-4
  • NCBIProtein:BAF66333.1

    Source assertion: The original GenPept BAF66333.1 identifies locus NWMN_0061 in Newman, taxon 426430, with its native sequence.

    Source locationGBSet/GBSeq[GBSeq_accession-version="BAF66333.1"]/GBSeq_feature-table; GBSeq_sequence
  • UniProtKB:Q2G1N2

    Source assertion: The NCTC 8325 entry assigns RHEA:51928 and RHEA:51929 with ECO:0000269. Its original sequence is identical to the selected Newman native protein; experimental constructs remain distinct.

    Source location/results/1/comments/1/reaction; physiologicalReactions; /results/1/sequence/value
  • DOI:10.14288/1.0228113

    Source assertion: Newman-derived SbnB is predominantly dimeric in solution; dynamic scattering and MALS support the assembly. This does not change the separate NCTC 8325 native-complex identity.

    Source locationResults 4.2.4, printed 98/PDF 120; Figure 4-7
NWMN_0064
NCBIProtein:BAF66336.1		enables

Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.

SbnE citryl-DAP ligation
RHEA:59125
  • PMID:19775248

    Source assertion: Purified SbnE ligates citrate to DAP, supported by substrate assays and isotope-shifted product masses. Newman coding regions were used; the reported free intermediates do not establish every selected ionic or stereochemical form.

    Source locationResults, SbnE citrate-desymmetrizing enzyme section; Figure7A; Experimental procedures, Protein overexpression and purification
  • NCBIProtein:BAF66336.1

    Source assertion: The original GenPept BAF66336.1 identifies locus NWMN_0064 in Newman, taxon 426430, with its native sequence.

    Source locationGBSet/GBSeq[GBSeq_accession-version="BAF66336.1"]/GBSeq_feature-table; GBSeq_sequence
  • UniProtKB:Q2G1M9

    Source assertion: The NCTC 8325 entry assigns RHEA:59124 and RHEA:59125 with ECO:0000269. Its original sequence is identical to the selected Newman native protein; experimental constructs remain distinct.

    Source location/results/4/comments/1/reaction; physiologicalReactions; /results/4/sequence/value
NWMN_0067
NCBIProtein:BAF66339.1		enables

Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.

SbnH citryl-DAP decarboxylation
RHEA:59121
  • PMID:19775248

    Source assertion: Addition of SbnH converts the citryl-DAP intermediate to a decarboxylated intermediate. The enzyme is reported as a PLP-dependent dimer, while no unique monomer sufficiency is established.

    Source locationResults, SbnH carries out PLP-dependent decarboxylation; Figure7B; Experimental procedures, Protein overexpression and purification
  • NCBIProtein:BAF66339.1

    Source assertion: The original GenPept BAF66339.1 identifies locus NWMN_0067 in Newman, taxon 426430, with its native sequence.

    Source locationGBSet/GBSeq[GBSeq_accession-version="BAF66339.1"]/GBSeq_feature-table; GBSeq_sequence
  • UniProtKB:Q2G1M6

    Source assertion: The NCTC 8325 entry assigns RHEA:59120 and RHEA:59121 with ECO:0000269. Its original sequence is identical to the selected Newman native protein; experimental constructs remain distinct.

    Source location/results/2/comments/1/reaction; physiologicalReactions; /results/2/sequence/value
NWMN_0065
NCBIProtein:BAF66337.1		enables

Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.

SbnF second DAP ligation
RHEA:59129
  • PMID:19775248

    Source assertion: SbnF adds the second DAP unit to citryl-diaminoethane in the proposed assembly sequence, supported by the intermediate mass and substrate assays. Solution monomer/dimer mixtures are reported.

    Source locationResults, SbnF is necessary to form a staphyloferrin B intermediate; Figure7C; Experimental procedures, Protein overexpression and purification
  • NCBIProtein:BAF66337.1

    Source assertion: The original GenPept BAF66337.1 identifies locus NWMN_0065 in Newman, taxon 426430, with its native sequence.

    Source locationGBSet/GBSeq[GBSeq_accession-version="BAF66337.1"]/GBSeq_feature-table; GBSeq_sequence
  • UniProtKB:Q2G1M8

    Source assertion: The NCTC 8325 entry assigns RHEA:59128 and RHEA:59129 with ECO:0000269. Its original sequence is identical to the selected Newman native protein; experimental constructs remain distinct.

    Source location/results/6/comments/1/reaction; physiologicalReactions; /results/6/sequence/value
NWMN_0062
NCBIProtein:BAF66334.1		enables

Newman gene-product activity with exact native sequence bridge. This functional relation does not claim an isolated monomer is sufficient. Full engineered construct sequence is not equated to the unmodified genome translation.

SbnC terminal ligation under the formal open-chain product convention
RHEA:59133
  • PMID:19775248

    Source assertion: SbnC is required for the final 2-oxoglutarate ligation and biologically active SB assembly. The measured mass does not distinguish the later characterized cyclic hemiaminal from the formal linear reaction convention.

    Source locationResults, SbnC activates α-KG in a reaction that completes the synthesis of staphyloferrin B; Figure7D; Experimental procedures, Protein overexpression and purification
  • NCBIProtein:BAF66334.1

    Source assertion: The original GenPept BAF66334.1 identifies locus NWMN_0062 in Newman, taxon 426430, with its native sequence.

    Source locationGBSet/GBSeq[GBSeq_accession-version="BAF66334.1"]/GBSeq_feature-table; GBSeq_sequence
  • UniProtKB:Q2G1N1

    Source assertion: The NCTC 8325 entry assigns RHEA:59132 and RHEA:59133 with ECO:0000269. Its original sequence is identical to the selected Newman native protein; experimental constructs remain distinct.

    Source location/results/7/comments/1/reaction; physiologicalReactions; /results/7/sequence/value
ATP-dependent L-serine kinase SbnI
UniProtKB:Q2G1M5		part_of

The named component and complex retain NCTC 8325 source identity; they are not renamed Newman entities.

L-serine kinase
MetaCyc:CPLX-9312
  • MetaCyc:CPLX-9312

    Source assertion: The native NCTC 8325 complex contains two copies of G1I0R-77-MONOMER; this is the source-defined assembly projection.

    Source locationProtein/component/Protein; component/coefficient; species/Organism
  • MetaCyc:G1I0R-77-MONOMER

    Source assertion: The native component explicitly cross-references UniProt Q2G1M5.

    Source locationProtein/dblink[dblink-db="UNIPROT"]/dblink-oid
L-serine kinase
MetaCyc:CPLX-9312		catalyzes

Curated NCTC 8325 assembly/activity assertion. SbnI oligomerization is not essential for kinase activity; SbnB solution dimer evidence is independently reported for Newman-derived protein.

SbnI free L-serine phosphorylation
RHEA:59113
  • MetaCyc:CPLX-9312

    Source assertion: The native complex has an enzymatic-reaction link to the represented native transformation; the Rhea reaction is selected via the separately documented chemistry bridge.

    Source locationProtein/catalyzes/Enzymatic-Reaction/reaction/Reaction
  • UniProtKB:Q2G1M5

    Source assertion: The component entry assigns the selected reaction and a homooligomeric assembly. Primary Newman experiments and exact sequence equality support comparison, not a change of source strain.

    Source location/results/0/comments (CATALYTIC ACTIVITY; SUBUNIT); /results/0/sequence/value
N-((2S)-2-amino-2-carboxyethyl)-L-glutamate dehydrogenase
UniProtKB:Q2G1N2		part_of

The named component and complex retain NCTC 8325 source identity; they are not renamed Newman entities.

N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate dehydrogenase
MetaCyc:CPLX-9313
  • MetaCyc:CPLX-9313

    Source assertion: The native NCTC 8325 complex contains two copies of G1I0R-70-MONOMER; this is the source-defined assembly projection.

    Source locationProtein/component/Protein; component/coefficient; species/Organism
  • MetaCyc:G1I0R-70-MONOMER

    Source assertion: The native component explicitly cross-references UniProt Q2G1N2.

    Source locationProtein/dblink[dblink-db="UNIPROT"]/dblink-oid
N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate dehydrogenase
MetaCyc:CPLX-9313		catalyzes

Curated NCTC 8325 assembly/activity assertion. SbnI oligomerization is not essential for kinase activity; SbnB solution dimer evidence is independently reported for Newman-derived protein.

SbnB oxidative ACEGA hydrolysis
RHEA:51929
  • MetaCyc:CPLX-9313

    Source assertion: The native complex has an enzymatic-reaction link to the represented native transformation; the Rhea reaction is selected via the separately documented chemistry bridge.

    Source locationProtein/catalyzes/Enzymatic-Reaction/reaction/Reaction
  • UniProtKB:Q2G1N2

    Source assertion: The component entry assigns the selected reaction and a homooligomeric assembly. Primary Newman experiments and exact sequence equality support comparison, not a change of source strain.

    Source location/results/1/comments (CATALYTIC ACTIVITY; SUBUNIT); /results/1/sequence/value
NWMN_0060
NCBIProtein:BAF66332.1		has_cofactor

pyridoxal 5'-phosphate(2-)
CHEBI:597326
  • PMID:26794841

    Source assertion: Purified Newman-derived SbnA is a dimer with bound PLP; crystallographic PLP binding is reported. The selected ChEBI charge state is the current chemical convention.

    Source locationsec2.1 paragraph1; sec3.1 paragraphs1-2; Figure1
  • UniProtKB:Q2G1N3

    Source assertion: The sequence-identical NCTC 8325 entry explicitly assigns pyridoxal phosphate CHEBI:597326 with ECO:0000269.

    Source location/results/3/comments/2/cofactors/0
NWMN_0067
NCBIProtein:BAF66339.1		has_cofactor

pyridoxal 5'-phosphate(2-)
CHEBI:597326
  • PMID:19775248

    Source assertion: SbnH is described as a PLP-dependent decarboxylase and its purified yellow protein is consistent with bound PLP; the selected charge state is a chemical convention.

    Source locationResults, SbnH carries out PLP-dependent decarboxylation, opening paragraph
  • UniProtKB:Q2G1M6

    Source assertion: The sequence-identical NCTC 8325 entry explicitly assigns pyridoxal phosphate CHEBI:597326 with ECO:0000269.

    Source location/results/2/comments/2/cofactors/0
L-serine kinase
MetaCyc:CPLX-9312		has_cofactor

Qualified native cofactor annotation. Newman ligase roles use exact sequence correspondence to the native NCTC 8325 gene product; the complete-system Mg omission experiment does not establish individual binding counts.

magnesium(2+)
CHEBI:18420
  • MetaCyc:ENZRXN-26580

    Source assertion: The native enzyme-activity annotation explicitly lists MG+2 as cofactor. This source-specific assignment does not establish a unique physiological metal or measured binding stoichiometry.

    Source locationEnzymatic-Reaction/enzyme/Protein; reaction/Reaction; cofactor/Compound[@frameid="MG+2"]
NWMN_0064
NCBIProtein:BAF66336.1		has_cofactor

Qualified native cofactor annotation. Newman ligase roles use exact sequence correspondence to the native NCTC 8325 gene product; the complete-system Mg omission experiment does not establish individual binding counts.

magnesium(2+)
CHEBI:18420
  • MetaCyc:ENZRXN-26574

    Source assertion: The native enzyme-activity annotation explicitly lists MG+2 as cofactor. This source-specific assignment does not establish a unique physiological metal or measured binding stoichiometry.

    Source locationEnzymatic-Reaction/enzyme/Protein; reaction/Reaction; cofactor/Compound[@frameid="MG+2"]
  • PMID:19775248

    Source assertion: Omitting Mg2+ from the complete SbnCEFH assembly prevented the SB ion; this tests the reaction system, not separate metal-binding stoichiometry for each ligase.

    Source locationResults, In vitro synthesis of staphyloferrin B requires three NIS synthetases and a decarboxylase, Mg omission statement; Figure3
NWMN_0065
NCBIProtein:BAF66337.1		has_cofactor

Qualified native cofactor annotation. Newman ligase roles use exact sequence correspondence to the native NCTC 8325 gene product; the complete-system Mg omission experiment does not establish individual binding counts.

magnesium(2+)
CHEBI:18420
  • MetaCyc:ENZRXN-26576

    Source assertion: The native enzyme-activity annotation explicitly lists MG+2 as cofactor. This source-specific assignment does not establish a unique physiological metal or measured binding stoichiometry.

    Source locationEnzymatic-Reaction/enzyme/Protein; reaction/Reaction; cofactor/Compound[@frameid="MG+2"]
  • PMID:19775248

    Source assertion: Omitting Mg2+ from the complete SbnCEFH assembly prevented the SB ion; this tests the reaction system, not separate metal-binding stoichiometry for each ligase.

    Source locationResults, In vitro synthesis of staphyloferrin B requires three NIS synthetases and a decarboxylase, Mg omission statement; Figure3
NWMN_0062
NCBIProtein:BAF66334.1		has_cofactor

Qualified native cofactor annotation. Newman ligase roles use exact sequence correspondence to the native NCTC 8325 gene product; the complete-system Mg omission experiment does not establish individual binding counts.

magnesium(2+)
CHEBI:18420
  • MetaCyc:ENZRXN-26577

    Source assertion: The native enzyme-activity annotation explicitly lists MG+2 as cofactor. This source-specific assignment does not establish a unique physiological metal or measured binding stoichiometry.

    Source locationEnzymatic-Reaction/enzyme/Protein; reaction/Reaction; cofactor/Compound[@frameid="MG+2"]
  • PMID:19775248

    Source assertion: Omitting Mg2+ from the complete SbnCEFH assembly prevented the SB ion; this tests the reaction system, not separate metal-binding stoichiometry for each ligase.

    Source locationResults, In vitro synthesis of staphyloferrin B requires three NIS synthetases and a decarboxylase, Mg omission statement; Figure3
SbnI free L-serine phosphorylation
RHEA:59113		provides_input_for

Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.

SbnA ACEGA formation
RHEA:52385
  • RHEA:59113

    Source assertion: CHEBI:57524 is an explicit product of the selected upstream direction.

    Source locationhttp://rdf.rhea-db.org/59112_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_59112_compound_1236; rh:compound -> http://rdf.rhea-db.org/Compound_1236; rh:chebi
  • RHEA:52385

    Source assertion: CHEBI:57524 is an explicit substrate of the selected downstream direction.

    Source locationhttp://rdf.rhea-db.org/52384_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_52384_compound_1236; rh:compound -> http://rdf.rhea-db.org/Compound_1236; rh:chebi
SbnA ACEGA formation
RHEA:52385		provides_input_for

Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.

SbnB oxidative ACEGA hydrolysis
RHEA:51929
  • RHEA:52385

    Source assertion: CHEBI:134610 is an explicit product of the selected upstream direction.

    Source locationhttp://rdf.rhea-db.org/52384_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_52384_compound_13099; rh:compound -> http://rdf.rhea-db.org/Compound_13099; rh:chebi
  • RHEA:51929

    Source assertion: CHEBI:134610 is an explicit substrate of the selected downstream direction.

    Source locationhttp://rdf.rhea-db.org/51928_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_13099; rh:compound -> http://rdf.rhea-db.org/Compound_13099; rh:chebi
SbnB oxidative ACEGA hydrolysis
RHEA:51929		provides_input_for

Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.

SbnE citryl-DAP ligation
RHEA:59125
  • RHEA:51929

    Source assertion: CHEBI:57721 is an explicit product of the selected upstream direction.

    Source locationhttp://rdf.rhea-db.org/51928_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_1905; rh:compound -> http://rdf.rhea-db.org/Compound_1905; rh:chebi
  • RHEA:59125

    Source assertion: CHEBI:57721 is an explicit substrate of the selected downstream direction.

    Source locationhttp://rdf.rhea-db.org/59124_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_59124_compound_1905; rh:compound -> http://rdf.rhea-db.org/Compound_1905; rh:chebi
SbnE citryl-DAP ligation
RHEA:59125		provides_input_for

Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.

SbnH citryl-DAP decarboxylation
RHEA:59121
  • RHEA:59125

    Source assertion: CHEBI:142969 is an explicit product of the selected upstream direction.

    Source locationhttp://rdf.rhea-db.org/59124_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_59124_compound_15297; rh:compound -> http://rdf.rhea-db.org/Compound_15297; rh:chebi
  • RHEA:59121

    Source assertion: CHEBI:142969 is an explicit substrate of the selected downstream direction.

    Source locationhttp://rdf.rhea-db.org/59120_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_59120_compound_15297; rh:compound -> http://rdf.rhea-db.org/Compound_15297; rh:chebi
SbnH citryl-DAP decarboxylation
RHEA:59121		provides_input_for

Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.

SbnF second DAP ligation
RHEA:59129
  • RHEA:59121

    Source assertion: CHEBI:142970 is an explicit product of the selected upstream direction.

    Source locationhttp://rdf.rhea-db.org/59120_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_59120_compound_15295; rh:compound -> http://rdf.rhea-db.org/Compound_15295; rh:chebi
  • RHEA:59129

    Source assertion: CHEBI:142970 is an explicit substrate of the selected downstream direction.

    Source locationhttp://rdf.rhea-db.org/59128_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_59128_compound_15295; rh:compound -> http://rdf.rhea-db.org/Compound_15295; rh:chebi
SbnB oxidative ACEGA hydrolysis
RHEA:51929		provides_input_for

Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.

SbnF second DAP ligation
RHEA:59129
  • RHEA:51929

    Source assertion: CHEBI:57721 is an explicit product of the selected upstream direction.

    Source locationhttp://rdf.rhea-db.org/51928_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_1905; rh:compound -> http://rdf.rhea-db.org/Compound_1905; rh:chebi
  • RHEA:59129

    Source assertion: CHEBI:57721 is an explicit substrate of the selected downstream direction.

    Source locationhttp://rdf.rhea-db.org/59128_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_59128_compound_1905; rh:compound -> http://rdf.rhea-db.org/Compound_1905; rh:chebi
SbnF second DAP ligation
RHEA:59129		provides_input_for

Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.

SbnC terminal ligation under the formal open-chain product convention
RHEA:59133
  • RHEA:59129

    Source assertion: CHEBI:142971 is an explicit product of the selected upstream direction.

    Source locationhttp://rdf.rhea-db.org/59128_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_59128_compound_15296; rh:compound -> http://rdf.rhea-db.org/Compound_15296; rh:chebi
  • RHEA:59133

    Source assertion: CHEBI:142971 is an explicit substrate of the selected downstream direction.

    Source locationhttp://rdf.rhea-db.org/59132_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_59132_compound_15296; rh:compound -> http://rdf.rhea-db.org/Compound_15296; rh:chebi
SbnB oxidative ACEGA hydrolysis
RHEA:51929		provides_input_for

Material availability in the selected network, not direct enzyme channeling or a measured flux ratio.

SbnC terminal ligation under the formal open-chain product convention
RHEA:59133
  • RHEA:51929

    Source assertion: CHEBI:16810 is an explicit product of the selected upstream direction.

    Source locationhttp://rdf.rhea-db.org/51928_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_51928_compound_2975; rh:compound -> http://rdf.rhea-db.org/Compound_2975; rh:chebi
  • RHEA:59133

    Source assertion: CHEBI:16810 is an explicit substrate of the selected downstream direction.

    Source locationhttp://rdf.rhea-db.org/59132_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_59132_compound_2975; rh:compound -> http://rdf.rhea-db.org/Compound_2975; rh:chebi

References

Curation history