DHB adenylation
MetaCyc:DHBAMPLIG-RXN | part_of Complete native member: DHB adenylation. | MetaCyc:PWY-8383 | |
DHB loading onto MxcF
MetaCyc:RXN-23310 | part_of Complete native member: DHB loading onto MxcF. | MetaCyc:PWY-8383 | |
L-lysine adenylation
MetaCyc:RXN-22679 | part_of Complete native member: L-lysine adenylation. | MetaCyc:PWY-8383 | |
L-lysine loading onto MxcG
MetaCyc:RXN-23308 | part_of Complete native member: L-lysine loading onto MxcG. | MetaCyc:PWY-8383 | |
first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | part_of Complete native member: first DHB condensation (native N6-first convention). N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | MetaCyc:PWY-8383 | - MetaCyc:PWY-8383
Source assertion: The native pathway directly includes RXN-23316 among nine reaction members. PWY-5901 is separately linked precursor supply, not a nested member. Source location/ptools-xml/Pathway[@frameid="PWY-8383"]/reaction-list/Reaction[@frameid="RXN-23316"]; /ptools-xml/Pathway/pathway-link - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
second DHB condensation
MetaCyc:RXN-23312 | part_of Complete native member: second DHB condensation. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | MetaCyc:PWY-8383 | - MetaCyc:PWY-8383
Source assertion: The native pathway directly includes RXN-23312 among nine reaction members. PWY-5901 is separately linked precursor supply, not a nested member. Source location/ptools-xml/Pathway[@frameid="PWY-8383"]/reaction-list/Reaction[@frameid="RXN-23312"]; /ptools-xml/Pathway/pathway-link - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | part_of Complete native member: reductive carrier release to the aldehyde. | MetaCyc:PWY-8383 | |
aldehyde reduction to myxochelin A
MetaCyc:RXN-23314 | part_of Complete native member: aldehyde reduction to myxochelin A. | MetaCyc:PWY-8383 | |
aldehyde transamination to myxochelin B
MetaCyc:RXN-23315 | part_of Complete native member: aldehyde transamination to myxochelin B. | MetaCyc:PWY-8383 | |
ATP(4-)
CHEBI:30616 | consumes Coefficient: 1. | DHB adenylation
MetaCyc:DHBAMPLIG-RXN | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The original left side contains ATP with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="DHBAMPLIG-RXN"]/left[*[@frameid="ATP"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:ATP
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="ATP"]/inchi - CHEBI:30616
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:30616" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge") - RHEA:20230
Source assertion: The current Rhea forward reaction independently preserves this exact chemical identity and unit coefficient, including diphosphate(3-) and one consumed hydron. It matches the selected native first reaction. Source locationRDF RHEA:20230/substrates -> http://rdf.rhea-db.org/20229_L/contains1 -> http://rdf.rhea-db.org/Participant_20229_compound_6372/compound -> http://rdf.rhea-db.org/Compound_6372/accession
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2,3-dihydroxybenzoate
CHEBI:36654 | consumes Coefficient: 1. | DHB adenylation
MetaCyc:DHBAMPLIG-RXN | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The original left side contains 2-3-DIHYDROXYBENZOATE with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="DHBAMPLIG-RXN"]/left[*[@frameid="2-3-DIHYDROXYBENZOATE"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:2-3-DIHYDROXYBENZOATE
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="2-3-DIHYDROXYBENZOATE"]/inchi - CHEBI:36654
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:36654" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge") - RHEA:20230
Source assertion: The current Rhea forward reaction independently preserves this exact chemical identity and unit coefficient, including diphosphate(3-) and one consumed hydron. It matches the selected native first reaction. Source locationRDF RHEA:20230/substrates -> http://rdf.rhea-db.org/20229_L/contains1 -> http://rdf.rhea-db.org/Participant_20229_compound_3751/compound -> http://rdf.rhea-db.org/Compound_3751/accession
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hydron
CHEBI:15378 | consumes Coefficient: 1. | DHB adenylation
MetaCyc:DHBAMPLIG-RXN | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The original left side contains PROTON with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="DHBAMPLIG-RXN"]/left[*[@frameid="PROTON"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PROTON
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PROTON"]/inchi - CHEBI:15378
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:15378" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge") - RHEA:20230
Source assertion: The current Rhea forward reaction independently preserves this exact chemical identity and unit coefficient, including diphosphate(3-) and one consumed hydron. It matches the selected native first reaction. Source locationRDF RHEA:20230/substrates -> http://rdf.rhea-db.org/20229_L/contains1 -> http://rdf.rhea-db.org/Participant_20229_compound_3249/compound -> http://rdf.rhea-db.org/Compound_3249/accession
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DHB adenylation
MetaCyc:DHBAMPLIG-RXN | produces Coefficient: 1. | 2,3-dihydroxybenzoyl 5'-adenylate(1-)
CHEBI:57417 | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The original right side contains CPD-62 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="DHBAMPLIG-RXN"]/right[*[@frameid="CPD-62"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:CPD-62
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="CPD-62"]/inchi - CHEBI:57417
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:57417" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge") - RHEA:20230
Source assertion: The current Rhea forward reaction independently preserves this exact chemical identity and unit coefficient, including diphosphate(3-) and one consumed hydron. It matches the selected native first reaction. Source locationRDF RHEA:20230/products -> http://rdf.rhea-db.org/20229_R/contains1 -> http://rdf.rhea-db.org/Participant_20229_compound_2580/compound -> http://rdf.rhea-db.org/Compound_2580/accession
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DHB adenylation
MetaCyc:DHBAMPLIG-RXN | produces Coefficient: 1. | diphosphate(3-)
CHEBI:33019 | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The original right side contains PPI with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="DHBAMPLIG-RXN"]/right[*[@frameid="PPI"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PPI
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PPI"]/inchi - CHEBI:33019
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:33019" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge") - RHEA:20230
Source assertion: The current Rhea forward reaction independently preserves this exact chemical identity and unit coefficient, including diphosphate(3-) and one consumed hydron. It matches the selected native first reaction. Source locationRDF RHEA:20230/products -> http://rdf.rhea-db.org/20229_R/contains1 -> http://rdf.rhea-db.org/Participant_20229_compound_2106/compound -> http://rdf.rhea-db.org/Compound_2106/accession
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2,3-dihydroxybenzoyl 5'-adenylate(1-)
CHEBI:57417 | consumes Coefficient: 1. | DHB loading onto MxcF
MetaCyc:RXN-23310 | - MetaCyc:RXN-23310
Source assertion: The original left side contains CPD-62 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23310"]/left[*[@frameid="CPD-62"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:CPD-62
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="CPD-62"]/inchi - CHEBI:57417
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:57417" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
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a holo-[MxcF aryl-carrier protein]
MetaCyc:MxcF | consumes Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. | DHB loading onto MxcF
MetaCyc:RXN-23310 | - MetaCyc:RXN-23310
Source assertion: The original left side contains MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23310"]/left[*[@frameid="MxcF"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:MxcF
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="MxcF"]/common-name
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DHB loading onto MxcF
MetaCyc:RXN-23310 | produces Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. | 2,3-dihydroxybenzoyl-[MxcF aryl-carrier protein]
MetaCyc:23-DHB-MxcF | - MetaCyc:RXN-23310
Source assertion: The original right side contains 23-DHB-MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23310"]/right[*[@frameid="23-DHB-MxcF"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:23-DHB-MxcF
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="23-DHB-MxcF"]/common-name
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DHB loading onto MxcF
MetaCyc:RXN-23310 | produces Coefficient: 1. | adenosine 5'-monophosphate(2-)
CHEBI:456215 | - MetaCyc:RXN-23310
Source assertion: The original right side contains AMP with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23310"]/right[*[@frameid="AMP"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:AMP
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="AMP"]/inchi - CHEBI:456215
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:456215" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
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DHB loading onto MxcF
MetaCyc:RXN-23310 | produces Coefficient: 1. | hydron
CHEBI:15378 | - MetaCyc:RXN-23310
Source assertion: The original right side contains PROTON with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23310"]/right[*[@frameid="PROTON"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PROTON
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PROTON"]/inchi - CHEBI:15378
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:15378" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
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L-lysinium(1+)
CHEBI:32551 | consumes Coefficient: 1. | L-lysine adenylation
MetaCyc:RXN-22679 | - MetaCyc:RXN-22679
Source assertion: The original left side contains LYS with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-22679"]/left[*[@frameid="LYS"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:LYS
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="LYS"]/inchi - CHEBI:32551
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:32551" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
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ATP(4-)
CHEBI:30616 | consumes Coefficient: 1. | L-lysine adenylation
MetaCyc:RXN-22679 | - MetaCyc:RXN-22679
Source assertion: The original left side contains ATP with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-22679"]/left[*[@frameid="ATP"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:ATP
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="ATP"]/inchi - CHEBI:30616
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:30616" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
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hydron
CHEBI:15378 | consumes Coefficient: 1. | L-lysine adenylation
MetaCyc:RXN-22679 | - MetaCyc:RXN-22679
Source assertion: The original left side contains PROTON with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-22679"]/left[*[@frameid="PROTON"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PROTON
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PROTON"]/inchi - CHEBI:15378
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:15378" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
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L-lysine adenylation
MetaCyc:RXN-22679 | produces Coefficient: 1. | L-lysyl-5'-AMP(1+)
CHEBI:176581 | - MetaCyc:RXN-22679
Source assertion: The original right side contains CPD-24910 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-22679"]/right[*[@frameid="CPD-24910"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:CPD-24910
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="CPD-24910"]/inchi - CHEBI:176581
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:176581" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
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L-lysine adenylation
MetaCyc:RXN-22679 | produces Coefficient: 1. | diphosphate(3-)
CHEBI:33019 | - MetaCyc:RXN-22679
Source assertion: The original right side contains PPI with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-22679"]/right[*[@frameid="PPI"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PPI
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PPI"]/inchi - CHEBI:33019
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:33019" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
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L-lysyl-5'-AMP(1+)
CHEBI:176581 | consumes Coefficient: 1. | L-lysine loading onto MxcG
MetaCyc:RXN-23308 | - MetaCyc:RXN-23308
Source assertion: The original left side contains CPD-24910 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23308"]/left[*[@frameid="CPD-24910"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:CPD-24910
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="CPD-24910"]/inchi - CHEBI:176581
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:176581" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
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a holo-[MxcG non-ribosomal peptide synthase]
MetaCyc:MxcG | consumes Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. | L-lysine loading onto MxcG
MetaCyc:RXN-23308 | - MetaCyc:RXN-23308
Source assertion: The original left side contains MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23308"]/left[*[@frameid="MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:MxcG
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="MxcG"]/common-name
|
L-lysine loading onto MxcG
MetaCyc:RXN-23308 | produces Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. | L-lysyl-[MxcG non-ribosomal peptide synthase]
MetaCyc:Lysyl-MxcG | - MetaCyc:RXN-23308
Source assertion: The original right side contains Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23308"]/right[*[@frameid="Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:Lysyl-MxcG
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="Lysyl-MxcG"]/common-name
|
L-lysine loading onto MxcG
MetaCyc:RXN-23308 | produces Coefficient: 1. | adenosine 5'-monophosphate(2-)
CHEBI:456215 | - MetaCyc:RXN-23308
Source assertion: The original right side contains AMP with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23308"]/right[*[@frameid="AMP"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:AMP
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="AMP"]/inchi - CHEBI:456215
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:456215" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
L-lysine loading onto MxcG
MetaCyc:RXN-23308 | produces Coefficient: 1. | hydron
CHEBI:15378 | - MetaCyc:RXN-23308
Source assertion: The original right side contains PROTON with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23308"]/right[*[@frameid="PROTON"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PROTON
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PROTON"]/inchi - CHEBI:15378
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:15378" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
2,3-dihydroxybenzoyl-[MxcF aryl-carrier protein]
MetaCyc:23-DHB-MxcF | consumes Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | - MetaCyc:RXN-23316
Source assertion: The original left side contains 23-DHB-MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23316"]/left[*[@frameid="23-DHB-MxcF"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:23-DHB-MxcF
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="23-DHB-MxcF"]/common-name - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
L-lysyl-[MxcG non-ribosomal peptide synthase]
MetaCyc:Lysyl-MxcG | consumes Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | - MetaCyc:RXN-23316
Source assertion: The original left side contains Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23316"]/left[*[@frameid="Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:Lysyl-MxcG
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="Lysyl-MxcG"]/common-name - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | produces Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | N6-(2,3-dihydsroxybenzoyl)-L-lysyl-[MxcG non-ribosomal peptide synthase]
MetaCyc:N-23-DHB-Lysyl-MxcG | - MetaCyc:RXN-23316
Source assertion: The original right side contains N-23-DHB-Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23316"]/right[*[@frameid="N-23-DHB-Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:N-23-DHB-Lysyl-MxcG
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="N-23-DHB-Lysyl-MxcG"]/common-name - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | produces Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | a holo-[MxcF aryl-carrier protein]
MetaCyc:MxcF | - MetaCyc:RXN-23316
Source assertion: The original right side contains MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23316"]/right[*[@frameid="MxcF"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:MxcF
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="MxcF"]/common-name - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | produces Coefficient: 1. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | hydron
CHEBI:15378 | - MetaCyc:RXN-23316
Source assertion: The original right side contains PROTON with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23316"]/right[*[@frameid="PROTON"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PROTON
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PROTON"]/inchi - CHEBI:15378
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:15378" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge") - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
2,3-dihydroxybenzoyl-[MxcF aryl-carrier protein]
MetaCyc:23-DHB-MxcF | consumes Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | second DHB condensation
MetaCyc:RXN-23312 | - MetaCyc:RXN-23312
Source assertion: The original left side contains 23-DHB-MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23312"]/left[*[@frameid="23-DHB-MxcF"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:23-DHB-MxcF
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="23-DHB-MxcF"]/common-name - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
N6-(2,3-dihydsroxybenzoyl)-L-lysyl-[MxcG non-ribosomal peptide synthase]
MetaCyc:N-23-DHB-Lysyl-MxcG | consumes Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | second DHB condensation
MetaCyc:RXN-23312 | - MetaCyc:RXN-23312
Source assertion: The original left side contains N-23-DHB-Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23312"]/left[*[@frameid="N-23-DHB-Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:N-23-DHB-Lysyl-MxcG
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="N-23-DHB-Lysyl-MxcG"]/common-name - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
second DHB condensation
MetaCyc:RXN-23312 | produces Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | N1,N6-(di-2,3-dihydroxybenzoyl)-L-lysyl-[MxcG non-ribosomal peptide synthase]
MetaCyc:N-N-di23-DHB-Lysyl-MxcG | - MetaCyc:RXN-23312
Source assertion: The original right side contains N-N-di23-DHB-Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23312"]/right[*[@frameid="N-N-di23-DHB-Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:N-N-di23-DHB-Lysyl-MxcG
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="N-N-di23-DHB-Lysyl-MxcG"]/common-name - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
second DHB condensation
MetaCyc:RXN-23312 | produces Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | a holo-[MxcF aryl-carrier protein]
MetaCyc:MxcF | - MetaCyc:RXN-23312
Source assertion: The original right side contains MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23312"]/right[*[@frameid="MxcF"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:MxcF
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="MxcF"]/common-name - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
second DHB condensation
MetaCyc:RXN-23312 | produces Coefficient: 1. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | hydron
CHEBI:15378 | - MetaCyc:RXN-23312
Source assertion: The original right side contains PROTON with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23312"]/right[*[@frameid="PROTON"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PROTON
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PROTON"]/inchi - CHEBI:15378
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:15378" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge") - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
|
N1,N6-(di-2,3-dihydroxybenzoyl)-L-lysyl-[MxcG non-ribosomal peptide synthase]
MetaCyc:N-N-di23-DHB-Lysyl-MxcG | consumes Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. | reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | - MetaCyc:RXN-23313
Source assertion: The original left side contains N-N-di23-DHB-Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23313"]/left[*[@frameid="N-N-di23-DHB-Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:N-N-di23-DHB-Lysyl-MxcG
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="N-N-di23-DHB-Lysyl-MxcG"]/common-name
|
NADPH(4-)
CHEBI:57783 | consumes Coefficient: 1. | reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | - MetaCyc:RXN-23313
Source assertion: The original left side contains NADPH with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23313"]/left[*[@frameid="NADPH"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:NADPH
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="NADPH"]/inchi - CHEBI:57783
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:57783" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
hydron
CHEBI:15378 | consumes Coefficient: 1. | reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | - MetaCyc:RXN-23313
Source assertion: The original left side contains PROTON with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23313"]/left[*[@frameid="PROTON"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PROTON
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PROTON"]/inchi - CHEBI:15378
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:15378" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | produces Coefficient: 1. | myxochelin aldehyde intermediate
MetaCyc:CPD-25916 | - MetaCyc:RXN-23313
Source assertion: The original right side contains CPD-25916 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23313"]/right[*[@frameid="CPD-25916"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:CPD-25916
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="CPD-25916"]/inchi
|
reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | produces Coefficient: 1. Whole carrier reactive class, not a free acyl group or a fixed number of native protein molecules. | a holo-[MxcG non-ribosomal peptide synthase]
MetaCyc:MxcG | - MetaCyc:RXN-23313
Source assertion: The original right side contains MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23313"]/right[*[@frameid="MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:MxcG
Source assertion: The original typed Protein class names this whole Mxc carrier reactive state. It does not supply a full molecular formula, engineered construct sequence or measured assembly stoichiometry. Source location/ptools-xml/Protein[@frameid="MxcG"]/common-name
|
reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | produces Coefficient: 1. | NADP(3-)
CHEBI:58349 | - MetaCyc:RXN-23313
Source assertion: The original right side contains NADP with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23313"]/right[*[@frameid="NADP"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:NADP
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="NADP"]/inchi - CHEBI:58349
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:58349" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
myxochelin aldehyde intermediate
MetaCyc:CPD-25916 | consumes Coefficient: 1. Native right-to-left flux reduces the aldehyde; the printed left side is the oxidation product side. | aldehyde reduction to myxochelin A
MetaCyc:RXN-23314 | - MetaCyc:RXN-23314
Source assertion: The original right side contains CPD-25916 with coefficient 1; native direction is PHYSIOL-RIGHT-TO-LEFT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23314"]/right[*[@frameid="CPD-25916"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:CPD-25916
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="CPD-25916"]/inchi
|
NADPH(4-)
CHEBI:57783 | consumes Coefficient: 1. Native right-to-left flux reduces the aldehyde; the printed left side is the oxidation product side. | aldehyde reduction to myxochelin A
MetaCyc:RXN-23314 | - MetaCyc:RXN-23314
Source assertion: The original right side contains NADPH with coefficient 1; native direction is PHYSIOL-RIGHT-TO-LEFT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23314"]/right[*[@frameid="NADPH"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:NADPH
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="NADPH"]/inchi - CHEBI:57783
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:57783" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
hydron
CHEBI:15378 | consumes Coefficient: 1. Native right-to-left flux reduces the aldehyde; the printed left side is the oxidation product side. | aldehyde reduction to myxochelin A
MetaCyc:RXN-23314 | - MetaCyc:RXN-23314
Source assertion: The original right side contains PROTON with coefficient 1; native direction is PHYSIOL-RIGHT-TO-LEFT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23314"]/right[*[@frameid="PROTON"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PROTON
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PROTON"]/inchi - CHEBI:15378
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:15378" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
aldehyde reduction to myxochelin A
MetaCyc:RXN-23314 | produces Coefficient: 1. The exact native stereospecific product is retained; stereounspecified CHEBI:31877 is not an exact mapping. Native right-to-left flux reduces the aldehyde; the printed left side is the oxidation product side. | myxochelin A
MetaCyc:CPD-25904 | - MetaCyc:RXN-23314
Source assertion: The original left side contains CPD-25904 with coefficient 1; native direction is PHYSIOL-RIGHT-TO-LEFT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23314"]/left[*[@frameid="CPD-25904"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:CPD-25904
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="CPD-25904"]/inchi
|
aldehyde reduction to myxochelin A
MetaCyc:RXN-23314 | produces Coefficient: 1. Native right-to-left flux reduces the aldehyde; the printed left side is the oxidation product side. | NADP(3-)
CHEBI:58349 | - MetaCyc:RXN-23314
Source assertion: The original left side contains NADP with coefficient 1; native direction is PHYSIOL-RIGHT-TO-LEFT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23314"]/left[*[@frameid="NADP"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:NADP
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="NADP"]/inchi - CHEBI:58349
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:58349" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
myxochelin aldehyde intermediate
MetaCyc:CPD-25916 | consumes Coefficient: 1. | aldehyde transamination to myxochelin B
MetaCyc:RXN-23315 | - MetaCyc:RXN-23315
Source assertion: The original left side contains CPD-25916 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23315"]/left[*[@frameid="CPD-25916"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:CPD-25916
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="CPD-25916"]/inchi
|
L-glutamate(1-)
CHEBI:29985 | consumes Coefficient: 1. | aldehyde transamination to myxochelin B
MetaCyc:RXN-23315 | - MetaCyc:RXN-23315
Source assertion: The original left side contains GLT with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23315"]/left[*[@frameid="GLT"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:GLT
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="GLT"]/inchi - CHEBI:29985
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:29985" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
aldehyde transamination to myxochelin B
MetaCyc:RXN-23315 | produces Coefficient: 1. CHEBI:7061 full structural identity is used; its inconsistent hydroxy/reduction definition is excluded. | myxochelin B
CHEBI:7061 | - MetaCyc:RXN-23315
Source assertion: The original right side contains CPD-25905 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23315"]/right[*[@frameid="CPD-25905"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:CPD-25905
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="CPD-25905"]/inchi - CHEBI:7061
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:7061" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
aldehyde transamination to myxochelin B
MetaCyc:RXN-23315 | produces Coefficient: 1. | 2-oxoglutarate(2-)
CHEBI:16810 | - MetaCyc:RXN-23315
Source assertion: The original right side contains 2-KETOGLUTARATE with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23315"]/right[*[@frameid="2-KETOGLUTARATE"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:2-KETOGLUTARATE
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="2-KETOGLUTARATE"]/inchi - CHEBI:16810
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:16810" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
aldehyde transamination to myxochelin B
MetaCyc:RXN-23315 | produces Coefficient: 1. | hydron
CHEBI:15378 | - MetaCyc:RXN-23315
Source assertion: The original right side contains PROTON with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23315"]/right[*[@frameid="PROTON"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:PROTON
Source assertion: The native compound supplies its explicit Standard InChI, fixing the source chemical structure and protonation used in this equation. Source location/ptools-xml/Compound[@frameid="PROTON"]/inchi - CHEBI:15378
Source assertion: The issuing ChEBI Standard InChI exactly matches the native compound, including stated stereochemistry and protonation. The identifier is selected by structure; descriptive definitions are not biochemical evidence. Source locationstatements WHERE subject="CHEBI:15378" AND predicate IN ("rdfs:label","chemrof:inchi_string","chemrof:generalized_empirical_formula","chemrof:charge")
|
MxcE (myxochelin biosynthesis protein)
UniProtKB:Q9F638 | enables Native gene-product/activity relation supported by source annotation and qualified recombinant reconstitution. No fixed E:F:G:L assembly is asserted. | DHB adenylation
MetaCyc:DHBAMPLIG-RXN | - NCBIProtein:AAG31128.1
Source assertion: The mxcE CDS in AF299336.1 supplies AAG31128.1, 543 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcE"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F638
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - MetaCyc:MONOMER-124161
Source assertion: The MxcE source object links Q9F638/AAG31128.1 and its composite DHB activation/loading activity RXN-23311. It is the myxochelin controller; the VibE controller of shared DHBAMPLIG-RXN is not transferred. Source location/ptools-xml/Protein/catalyzes/Enzymatic-Reaction/reaction; /ptools-xml/Protein/dblink - PMID:11562468
Source assertion: Discussion assigns DHB adenylation and loading onto the MxcF aryl-carrier domain to MxcE. The primary E/F/G reconstitution confirms pathway activity, rather than separately measuring each native half-reaction. Source location//*[@id="sec11"]/p[5]; //*[@id="sec9"]/p[1] - PMID:11562468
Source assertion: E/F/G genes were amplified from Sg a15 DNA using AF299336 primers. Intein-chitin constructs carry C-terminal extensions; purification and processing distinguish assayed proteins from exact bare native sequences. Source location//*[@id="sec2"]/p[1]; //*[@id="sec3"]/p[1]
|
MxcE (myxochelin biosynthesis protein)
UniProtKB:Q9F638 | enables Native gene-product/activity relation supported by source annotation and qualified recombinant reconstitution. No fixed E:F:G:L assembly is asserted. | DHB loading onto MxcF
MetaCyc:RXN-23310 | - NCBIProtein:AAG31128.1
Source assertion: The mxcE CDS in AF299336.1 supplies AAG31128.1, 543 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcE"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F638
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - MetaCyc:MONOMER-124161
Source assertion: The MxcE source object links Q9F638/AAG31128.1 and its composite DHB activation/loading activity RXN-23311. It is the myxochelin controller; the VibE controller of shared DHBAMPLIG-RXN is not transferred. Source location/ptools-xml/Protein/catalyzes/Enzymatic-Reaction/reaction; /ptools-xml/Protein/dblink - PMID:11562468
Source assertion: Discussion assigns DHB adenylation and loading onto the MxcF aryl-carrier domain to MxcE. The primary E/F/G reconstitution confirms pathway activity, rather than separately measuring each native half-reaction. Source location//*[@id="sec11"]/p[5]; //*[@id="sec9"]/p[1] - PMID:11562468
Source assertion: E/F/G genes were amplified from Sg a15 DNA using AF299336 primers. Intein-chitin constructs carry C-terminal extensions; purification and processing distinguish assayed proteins from exact bare native sequences. Source location//*[@id="sec2"]/p[1]; //*[@id="sec3"]/p[1]
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MxcG (myxochelin biosynthesis protein)
UniProtKB:Q9F636 | enables Native gene-product/activity relation supported by source annotation and qualified recombinant reconstitution. No fixed E:F:G:L assembly is asserted. | L-lysine adenylation
MetaCyc:RXN-22679 | - NCBIProtein:AAG31130.1
Source assertion: The mxcG CDS in AF299336.1 supplies AAG31130.1, 1456 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcG"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F636
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - MetaCyc:MONOMER-124162
Source assertion: The MxcG object links Q9F636/AAG31130.1 and assigns lysine loading, iterative condensation, carrier reduction and aldehyde reduction to its domains. Source location/ptools-xml/Protein/catalyzes; /ptools-xml/Protein/comment; /ptools-xml/Protein/dblink - PMID:11562468
Source assertion: The reconstituted E/F/G preparation forms A. Discussion assigns lysine activation/loading and two condensations to MxcG, and terminal two-stage reduction to its reductase domain. NADH also supports formation more slowly than NADPH. Source location//*[@id="sec9"]/p[1]; //*[@id="sec11"]/p[3]; //*[@id="sec11"]/p[5] - PMID:11562468
Source assertion: E/F/G genes were amplified from Sg a15 DNA using AF299336 primers. Intein-chitin constructs carry C-terminal extensions; purification and processing distinguish assayed proteins from exact bare native sequences. Source location//*[@id="sec2"]/p[1]; //*[@id="sec3"]/p[1]
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MxcG (myxochelin biosynthesis protein)
UniProtKB:Q9F636 | enables Native gene-product/activity relation supported by source annotation and qualified recombinant reconstitution. No fixed E:F:G:L assembly is asserted. | L-lysine loading onto MxcG
MetaCyc:RXN-23308 | - NCBIProtein:AAG31130.1
Source assertion: The mxcG CDS in AF299336.1 supplies AAG31130.1, 1456 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcG"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F636
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - MetaCyc:MONOMER-124162
Source assertion: The MxcG object links Q9F636/AAG31130.1 and assigns lysine loading, iterative condensation, carrier reduction and aldehyde reduction to its domains. Source location/ptools-xml/Protein/catalyzes; /ptools-xml/Protein/comment; /ptools-xml/Protein/dblink - PMID:11562468
Source assertion: The reconstituted E/F/G preparation forms A. Discussion assigns lysine activation/loading and two condensations to MxcG, and terminal two-stage reduction to its reductase domain. NADH also supports formation more slowly than NADPH. Source location//*[@id="sec9"]/p[1]; //*[@id="sec11"]/p[3]; //*[@id="sec11"]/p[5] - PMID:11562468
Source assertion: E/F/G genes were amplified from Sg a15 DNA using AF299336 primers. Intein-chitin constructs carry C-terminal extensions; purification and processing distinguish assayed proteins from exact bare native sequences. Source location//*[@id="sec2"]/p[1]; //*[@id="sec3"]/p[1]
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MxcG (myxochelin biosynthesis protein)
UniProtKB:Q9F636 | enables Native gene-product/activity relation supported by source annotation and qualified recombinant reconstitution. No fixed E:F:G:L assembly is asserted. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | - NCBIProtein:AAG31130.1
Source assertion: The mxcG CDS in AF299336.1 supplies AAG31130.1, 1456 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcG"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F636
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - MetaCyc:MONOMER-124162
Source assertion: The MxcG object links Q9F636/AAG31130.1 and assigns lysine loading, iterative condensation, carrier reduction and aldehyde reduction to its domains. Source location/ptools-xml/Protein/catalyzes; /ptools-xml/Protein/comment; /ptools-xml/Protein/dblink - PMID:11562468
Source assertion: The reconstituted E/F/G preparation forms A. Discussion assigns lysine activation/loading and two condensations to MxcG, and terminal two-stage reduction to its reductase domain. NADH also supports formation more slowly than NADPH. Source location//*[@id="sec9"]/p[1]; //*[@id="sec11"]/p[3]; //*[@id="sec11"]/p[5] - PMID:11562468
Source assertion: E/F/G genes were amplified from Sg a15 DNA using AF299336 primers. Intein-chitin constructs carry C-terminal extensions; purification and processing distinguish assayed proteins from exact bare native sequences. Source location//*[@id="sec2"]/p[1]; //*[@id="sec3"]/p[1] - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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MxcG (myxochelin biosynthesis protein)
UniProtKB:Q9F636 | enables Native gene-product/activity relation supported by source annotation and qualified recombinant reconstitution. No fixed E:F:G:L assembly is asserted. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | second DHB condensation
MetaCyc:RXN-23312 | - NCBIProtein:AAG31130.1
Source assertion: The mxcG CDS in AF299336.1 supplies AAG31130.1, 1456 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcG"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F636
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - MetaCyc:MONOMER-124162
Source assertion: The MxcG object links Q9F636/AAG31130.1 and assigns lysine loading, iterative condensation, carrier reduction and aldehyde reduction to its domains. Source location/ptools-xml/Protein/catalyzes; /ptools-xml/Protein/comment; /ptools-xml/Protein/dblink - PMID:11562468
Source assertion: The reconstituted E/F/G preparation forms A. Discussion assigns lysine activation/loading and two condensations to MxcG, and terminal two-stage reduction to its reductase domain. NADH also supports formation more slowly than NADPH. Source location//*[@id="sec9"]/p[1]; //*[@id="sec11"]/p[3]; //*[@id="sec11"]/p[5] - PMID:11562468
Source assertion: E/F/G genes were amplified from Sg a15 DNA using AF299336 primers. Intein-chitin constructs carry C-terminal extensions; purification and processing distinguish assayed proteins from exact bare native sequences. Source location//*[@id="sec2"]/p[1]; //*[@id="sec3"]/p[1] - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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MxcG (myxochelin biosynthesis protein)
UniProtKB:Q9F636 | enables Native gene-product/activity relation supported by source annotation and qualified recombinant reconstitution. No fixed E:F:G:L assembly is asserted. | reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | - NCBIProtein:AAG31130.1
Source assertion: The mxcG CDS in AF299336.1 supplies AAG31130.1, 1456 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcG"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F636
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - MetaCyc:MONOMER-124162
Source assertion: The MxcG object links Q9F636/AAG31130.1 and assigns lysine loading, iterative condensation, carrier reduction and aldehyde reduction to its domains. Source location/ptools-xml/Protein/catalyzes; /ptools-xml/Protein/comment; /ptools-xml/Protein/dblink - PMID:11562468
Source assertion: The reconstituted E/F/G preparation forms A. Discussion assigns lysine activation/loading and two condensations to MxcG, and terminal two-stage reduction to its reductase domain. NADH also supports formation more slowly than NADPH. Source location//*[@id="sec9"]/p[1]; //*[@id="sec11"]/p[3]; //*[@id="sec11"]/p[5] - DOI:10.1021/ja8025278
Source assertion: The complete enzyme preparation produces A and B; low amounts of free aldehyde were detected and characterized by LC-MS/MS and PFBHA derivatization. Source locationS2-S3 Reconstitution in vitro; S3 Detection of the aldehyde intermediate; S4 Figure S-3 - PMID:11562468
Source assertion: E/F/G genes were amplified from Sg a15 DNA using AF299336 primers. Intein-chitin constructs carry C-terminal extensions; purification and processing distinguish assayed proteins from exact bare native sequences. Source location//*[@id="sec2"]/p[1]; //*[@id="sec3"]/p[1]
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MxcG (myxochelin biosynthesis protein)
UniProtKB:Q9F636 | enables Native gene-product/activity relation supported by source annotation and qualified recombinant reconstitution. No fixed E:F:G:L assembly is asserted. | aldehyde reduction to myxochelin A
MetaCyc:RXN-23314 | - NCBIProtein:AAG31130.1
Source assertion: The mxcG CDS in AF299336.1 supplies AAG31130.1, 1456 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcG"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F636
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - MetaCyc:MONOMER-124162
Source assertion: The MxcG object links Q9F636/AAG31130.1 and assigns lysine loading, iterative condensation, carrier reduction and aldehyde reduction to its domains. Source location/ptools-xml/Protein/catalyzes; /ptools-xml/Protein/comment; /ptools-xml/Protein/dblink - PMID:11562468
Source assertion: The reconstituted E/F/G preparation forms A. Discussion assigns lysine activation/loading and two condensations to MxcG, and terminal two-stage reduction to its reductase domain. NADH also supports formation more slowly than NADPH. Source location//*[@id="sec9"]/p[1]; //*[@id="sec11"]/p[3]; //*[@id="sec11"]/p[5] - DOI:10.1021/ja8025278
Source assertion: The complete enzyme preparation produces A and B; low amounts of free aldehyde were detected and characterized by LC-MS/MS and PFBHA derivatization. Source locationS2-S3 Reconstitution in vitro; S3 Detection of the aldehyde intermediate; S4 Figure S-3 - PMID:11562468
Source assertion: E/F/G genes were amplified from Sg a15 DNA using AF299336 primers. Intein-chitin constructs carry C-terminal extensions; purification and processing distinguish assayed proteins from exact bare native sequences. Source location//*[@id="sec2"]/p[1]; //*[@id="sec3"]/p[1]
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MxcL (myxochelin biosynthesis protein)
UniProtKB:Q9F631 | enables Native gene-product/activity relation supported by source annotation and qualified recombinant reconstitution. No fixed E:F:G:L assembly is asserted. The 2008 tagged preparation is linked through the cited earlier plasmid; its complete engineered sequence was not independently reconstructed. | aldehyde transamination to myxochelin B
MetaCyc:RXN-23315 | - NCBIProtein:AAG31135.1
Source assertion: The mxcL CDS in AF299336.1 supplies AAG31135.1, 420 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcL"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F631
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - MetaCyc:MONOMER-124163
Source assertion: The source links Q9F631/AAG31135.1 in Sg a15 to the MxcL aminotransferase and ENZRXN-31013/RXN-23315. It describes the later recombinant E/F/G/L reconstitution; the 2001 paper only proposed this function. Source location/ptools-xml/Protein/catalyzes/Enzymatic-Reaction/reaction; /ptools-xml/Protein/comment; /ptools-xml/Protein/dblink - DOI:10.1021/ja8025278
Source assertion: His6-MxcL was amplified from pCBP-MxcL and expressed in E. coli. E/F/G/L with glutamate produces A and B; free aldehyde is detected. The earlier pCBP construct is cited, not fully sequenced in this supplement. Source locationS1 Cloning of mxcL; S1-S2 Overexpression and purification; S2-S3 Reconstitution in vitro; S3-S4 aldehyde analysis
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MxcF (aryl-carrier protein)
UniProtKB:Q9F637 | enables Carrier contribution, not independent condensation catalysis or a fixed physical stoichiometry. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | - NCBIProtein:AAG31129.1
Source assertion: The mxcF CDS in AF299336.1 supplies AAG31129.1, 311 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcF"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F637
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - PMID:11562468
Source assertion: The MxcF aryl-carrier delivers activated DHB twice. The paper leaves reuse of one physical carrier versus two separately loaded carriers unresolved. Source location//*[@id="sec11"]/p[5] - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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MxcF (aryl-carrier protein)
UniProtKB:Q9F637 | enables Carrier contribution, not independent condensation catalysis or a fixed physical stoichiometry. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | second DHB condensation
MetaCyc:RXN-23312 | - NCBIProtein:AAG31129.1
Source assertion: The mxcF CDS in AF299336.1 supplies AAG31129.1, 311 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcF"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F637
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism - PMID:11562468
Source assertion: The MxcF aryl-carrier delivers activated DHB twice. The paper leaves reuse of one physical carrier versus two separately loaded carriers unresolved. Source location//*[@id="sec11"]/p[5] - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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MxcF (aryl-carrier protein)
UniProtKB:Q9F637 | has_cofactor Covalent carrier prosthetic-group role. Heterologous MtaA maturation is supporting context outside the nine-member route; its identity is not transferred to a native activation edge. | pantetheine 4'-phosphate(2-)
CHEBI:47942 | - UniProtKB:Q9F637
Source assertion: The current unreviewed entry annotates covalent phosphopantetheine association with ECO:0000256 rule-based evidence, not an independent native-strain binding assay. Source location/comments/1/cofactors - PMID:11562468
Source assertion: MtaA coexpression increases MxcF and separately expressed PCPG mass by about 340 Da. This directly supports phosphopantetheinylation of these constructs, not measured full-length MxcG occupancy or an identified native Sg a15 PPTase. Source location//*[@id="sec8"]/p[1]; //*[@id="sec5"]/p[1] - NCBIProtein:AAG31129.1
Source assertion: The mxcF CDS in AF299336.1 supplies AAG31129.1, 311 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcF"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F637
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism
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MxcG (myxochelin biosynthesis protein)
UniProtKB:Q9F636 | has_cofactor Covalent carrier prosthetic-group role. Heterologous MtaA maturation is supporting context outside the nine-member route; its identity is not transferred to a native activation edge. | pantetheine 4'-phosphate(2-)
CHEBI:47942 | - UniProtKB:Q9F636
Source assertion: The current unreviewed entry annotates covalent phosphopantetheine association with ECO:0000256 rule-based evidence, not an independent native-strain binding assay. Source location/comments/0/cofactors - PMID:11562468
Source assertion: MtaA coexpression increases MxcF and separately expressed PCPG mass by about 340 Da. This directly supports phosphopantetheinylation of these constructs, not measured full-length MxcG occupancy or an identified native Sg a15 PPTase. Source location//*[@id="sec8"]/p[1]; //*[@id="sec5"]/p[1] - NCBIProtein:AAG31130.1
Source assertion: The mxcG CDS in AF299336.1 supplies AAG31130.1, 1456 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcG"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F636
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism
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MxcL (myxochelin biosynthesis protein)
UniProtKB:Q9F631 | has_cofactor PLP-dependent aminotransferase model with direct tagged-preparation spectroscopy and separately inferred native annotation; no cofactor stoichiometry claimed. | pyridoxal 5'-phosphate(2-)
CHEBI:597326 | - UniProtKB:Q9F631
Source assertion: The unreviewed entry annotates pyridoxal 5-prime-phosphate with ECO:0000256 ARBA evidence. This is distinct from the experimental tagged-preparation spectroscopy. Source location/comments/0/cofactors - DOI:10.1021/ja8025278
Source assertion: His6-MxcL incubated with PLP was buffer-exchanged before spectroscopy; addition of glutamate changes the PLP/PMP-associated UV spectrum. Source locationS2 Spectrophotometric measurement of PLP-bound MxcL and Figure S-1 - NCBIProtein:AAG31135.1
Source assertion: The mxcL CDS in AF299336.1 supplies AAG31135.1, 420 residues, from Sg a15. Its native translation is exactly identical to the selected UniProt sequence; engineered tags and processing are separate. Source location//GBFeature[GBFeature_key="CDS"][GBFeature_quals/GBQualifier[GBQualifier_name="gene"]/GBQualifier_value="mxcL"]/GBFeature_quals; //GBFeature[GBFeature_key="source"]/GBFeature_quals - UniProtKB:Q9F631
Source assertion: The entry provides the selected full native sequence and versioned EMBL protein cross-reference. This unreviewed species-level annotation is not itself an experimental assay. Source location/primaryAccession; /sequence; /uniProtKBCrossReferences; /entryType; /organism
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DHB adenylation
MetaCyc:DHBAMPLIG-RXN | provides_input_for Shared material: CHEBI:57417. This does not establish physical channeling or simultaneous carrier occupancy. | DHB loading onto MxcF
MetaCyc:RXN-23310 | - MetaCyc:DHBAMPLIG-RXN
Source assertion: The original right side contains CPD-62 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="DHBAMPLIG-RXN"]/right[*[@frameid="CPD-62"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:RXN-23310
Source assertion: The original left side contains CPD-62 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23310"]/left[*[@frameid="CPD-62"]]; /ptools-xml/Reaction/reaction-direction
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L-lysine adenylation
MetaCyc:RXN-22679 | provides_input_for Shared material: CHEBI:176581. This does not establish physical channeling or simultaneous carrier occupancy. | L-lysine loading onto MxcG
MetaCyc:RXN-23308 | - MetaCyc:RXN-22679
Source assertion: The original right side contains CPD-24910 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-22679"]/right[*[@frameid="CPD-24910"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:RXN-23308
Source assertion: The original left side contains CPD-24910 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23308"]/left[*[@frameid="CPD-24910"]]; /ptools-xml/Reaction/reaction-direction
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DHB loading onto MxcF
MetaCyc:RXN-23310 | provides_input_for Shared material: MetaCyc:23-DHB-MxcF. This does not establish physical channeling or simultaneous carrier occupancy. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | - MetaCyc:RXN-23310
Source assertion: The original right side contains 23-DHB-MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23310"]/right[*[@frameid="23-DHB-MxcF"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:RXN-23316
Source assertion: The original left side contains 23-DHB-MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23316"]/left[*[@frameid="23-DHB-MxcF"]]; /ptools-xml/Reaction/reaction-direction - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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DHB loading onto MxcF
MetaCyc:RXN-23310 | provides_input_for Shared material: MetaCyc:23-DHB-MxcF. This does not establish physical channeling or simultaneous carrier occupancy. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | second DHB condensation
MetaCyc:RXN-23312 | - MetaCyc:RXN-23310
Source assertion: The original right side contains 23-DHB-MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23310"]/right[*[@frameid="23-DHB-MxcF"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:RXN-23312
Source assertion: The original left side contains 23-DHB-MxcF with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23312"]/left[*[@frameid="23-DHB-MxcF"]]; /ptools-xml/Reaction/reaction-direction - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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L-lysine loading onto MxcG
MetaCyc:RXN-23308 | provides_input_for Shared material: MetaCyc:Lysyl-MxcG. This does not establish physical channeling or simultaneous carrier occupancy. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | - MetaCyc:RXN-23308
Source assertion: The original right side contains Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23308"]/right[*[@frameid="Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:RXN-23316
Source assertion: The original left side contains Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23316"]/left[*[@frameid="Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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first DHB condensation (native N6-first convention)
MetaCyc:RXN-23316 | provides_input_for Shared material: MetaCyc:N-23-DHB-Lysyl-MxcG. This does not establish physical channeling or simultaneous carrier occupancy. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | second DHB condensation
MetaCyc:RXN-23312 | - MetaCyc:RXN-23316
Source assertion: The original right side contains N-23-DHB-Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23316"]/right[*[@frameid="N-23-DHB-Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:RXN-23312
Source assertion: The original left side contains N-23-DHB-Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23312"]/left[*[@frameid="N-23-DHB-Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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second DHB condensation
MetaCyc:RXN-23312 | provides_input_for Shared material: MetaCyc:N-N-di23-DHB-Lysyl-MxcG. This does not establish physical channeling or simultaneous carrier occupancy. N6-first is the selected native database convention; the first amidated lysine nitrogen is experimentally unresolved, not demonstrated to be N6 or exclusive. | reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | - MetaCyc:RXN-23312
Source assertion: The original right side contains N-N-di23-DHB-Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23312"]/right[*[@frameid="N-N-di23-DHB-Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:RXN-23313
Source assertion: The original left side contains N-N-di23-DHB-Lysyl-MxcG with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23313"]/left[*[@frameid="N-N-di23-DHB-Lysyl-MxcG"]]; /ptools-xml/Reaction/reaction-direction - PMID:11562468
Source assertion: Figure 5C states that it is not known which lysine amide forms first. It supports both condensations but does not experimentally establish the selected native N6-first intermediate. Source location//*[@id="F5"]/figcaption/p
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reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | provides_input_for Shared material: MetaCyc:CPD-25916. This does not establish physical channeling or simultaneous carrier occupancy. | aldehyde reduction to myxochelin A
MetaCyc:RXN-23314 | - MetaCyc:RXN-23313
Source assertion: The original right side contains CPD-25916 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23313"]/right[*[@frameid="CPD-25916"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:RXN-23314
Source assertion: The original right side contains CPD-25916 with coefficient 1; native direction is PHYSIOL-RIGHT-TO-LEFT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23314"]/right[*[@frameid="CPD-25916"]]; /ptools-xml/Reaction/reaction-direction - DOI:10.1021/ja8025278
Source assertion: Low amounts of a free aldehyde intermediate were detected by LC-MS/MS and PFBHA derivatization in the complete reconstitution. Both product branches are retained. Source locationS3 Detection of the aldehyde intermediate; S4 Figure S-3
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reductive carrier release to the aldehyde
MetaCyc:RXN-23313 | provides_input_for Shared material: MetaCyc:CPD-25916. This does not establish physical channeling or simultaneous carrier occupancy. | aldehyde transamination to myxochelin B
MetaCyc:RXN-23315 | - MetaCyc:RXN-23313
Source assertion: The original right side contains CPD-25916 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected output in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23313"]/right[*[@frameid="CPD-25916"]]; /ptools-xml/Reaction/reaction-direction - MetaCyc:RXN-23315
Source assertion: The original left side contains CPD-25916 with coefficient 1; native direction is PHYSIOL-LEFT-TO-RIGHT. This is the selected input in pathway flux. Source location/ptools-xml/Reaction[@frameid="RXN-23315"]/left[*[@frameid="CPD-25916"]]; /ptools-xml/Reaction/reaction-direction - DOI:10.1021/ja8025278
Source assertion: Low amounts of a free aldehyde intermediate were detected by LC-MS/MS and PFBHA derivatization in the complete reconstitution. Both product branches are retained. Source locationS3 Detection of the aldehyde intermediate; S4 Figure S-3
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