acetate and ATP formation from acetyl-CoA I

MetaCyc:PWY0-1312

Acetate and ATP formation from acetyl-CoA I converts acetyl-CoA to acetate through acetyl phosphate while producing ATP from ADP.

Pathway type

fermentation

Organism scope

Thermotoga maritima NCBITaxon:2336

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Pathway network

16 nodes · 17 asserted directed edges

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Reaction / activity
Chemical / cofactor
Protein / complex
Other participant
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Thermotoga maritima `NCBITaxon:2336`	taxon
phosphate acetyltransferase `EC:2.3.1.8`	molecular activity
acetate kinase `EC:2.7.2.1`	molecular activity
acetyl-CoA `CHEBI:57288`	small molecule
phosphate `CHEBI:43474`	small molecule
acetyl phosphate `CHEBI:22191`	small molecule
CoA `CHEBI:57287`	small molecule
ADP `CHEBI:456216`	small molecule
acetate `CHEBI:30089`	small molecule
ATP `CHEBI:30616`	small molecule
Acetate kinase `UniProtKB:Q9WYB1`	protein
cytoplasm `GO:0005737`	cellular component
magnesium(2+) `CHEBI:18420`	cofactor
manganese(2+) `CHEBI:29035`	cofactor
Phosphate acetyltransferase `UniProtKB:Q9X0L4`	protein
phosphate acetyltransferase reaction `RHEA:19522`	reaction	left to right
acetate kinase reaction `RHEA:11354`	reaction	right to left

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
phosphate `CHEBI:43474`	consumes	phosphate acetyltransferase reaction `RHEA:19522`	RHEA:19522 Source assertion: Rhea lists CHEBI:43474 on the substrates side of RHEA:19522 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/19522']/rh:substrates; rh:contains/rh:compound/rh:accession`
acetyl-CoA `CHEBI:57288`	consumes	phosphate acetyltransferase reaction `RHEA:19522`	RHEA:19522 Source assertion: Rhea lists CHEBI:57288 on the substrates side of RHEA:19522 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/19522']/rh:substrates; rh:contains/rh:compound/rh:accession`
phosphate acetyltransferase reaction `RHEA:19522`	produces	acetyl phosphate `CHEBI:22191`	RHEA:19522 Source assertion: Rhea lists CHEBI:22191 on the products side of RHEA:19522 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/19522']/rh:products; rh:contains/rh:compound/rh:accession`
phosphate acetyltransferase reaction `RHEA:19522`	produces	CoA `CHEBI:57287`	RHEA:19522 Source assertion: Rhea lists CHEBI:57287 on the products side of RHEA:19522 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/19522']/rh:products; rh:contains/rh:compound/rh:accession`
acetyl phosphate `CHEBI:22191`	consumes	acetate kinase reaction `RHEA:11354`	RHEA:11354 Source assertion: Rhea lists CHEBI:22191 on the substrates side of RHEA:11354 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/11354']/rh:substrates; rh:contains/rh:compound/rh:accession`
ADP `CHEBI:456216`	consumes	acetate kinase reaction `RHEA:11354`	RHEA:11354 Source assertion: Rhea lists CHEBI:456216 on the substrates side of RHEA:11354 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/11354']/rh:substrates; rh:contains/rh:compound/rh:accession`
acetate kinase reaction `RHEA:11354`	produces	acetate `CHEBI:30089`	RHEA:11354 Source assertion: Rhea lists CHEBI:30089 on the products side of RHEA:11354 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/11354']/rh:products; rh:contains/rh:compound/rh:accession`
acetate kinase reaction `RHEA:11354`	produces	ATP `CHEBI:30616`	RHEA:11354 Source assertion: Rhea lists CHEBI:30616 on the products side of RHEA:11354 in this directed reaction. Source location `rdf:Description[@rdf:about='http://rdf.rhea-db.org/11354']/rh:products; rh:contains/rh:compound/rh:accession`
phosphate acetyltransferase `EC:2.3.1.8`	catalyzes	phosphate acetyltransferase reaction `RHEA:19522`	RHEA:19521 Source assertion: Rhea associates EC:2.3.1.8 with the reaction family containing RHEA:19522. Source location `rh:ec` PMID:10074080 Phosphate acetyltransferase (PTA) and acetate kinase (AK) of the hyperthermophilic eubacterium Thermotoga maritima have been purified 1,500- and 250-fold, respectively, to apparent homogeneity. Source location `PubMed Abstract`
acetate kinase `EC:2.7.2.1`	catalyzes	acetate kinase reaction `RHEA:11354`	RHEA:11352 Source assertion: Rhea associates EC:2.7.2.1 with the reaction family containing RHEA:11354. Source location `rh:ec` PMID:10074080 Phosphate acetyltransferase (PTA) and acetate kinase (AK) of the hyperthermophilic eubacterium Thermotoga maritima have been purified 1,500- and 250-fold, respectively, to apparent homogeneity. Source location `PubMed Abstract`
phosphate acetyltransferase reaction `RHEA:19522`	precedes	acetate kinase reaction `RHEA:11354`	MetaCyc:PWY0-1312 Source assertion: MetaCyc pathway ordering places RHEA:19522 before RHEA:11354 in this pathway. Shared intermediate: CHEBI:22191. Source location `Pathway/reaction-ordering and reaction-layout; Rhea-to-MetaCyc cross-references`
Acetate kinase `UniProtKB:Q9WYB1`	enables	acetate kinase reaction `RHEA:11354`	UniProtKB:Q9WYB1 Source assertion: UniProt annotates UniProtKB:Q9WYB1 with catalytic activity for the reaction family of RHEA:11354. Annotation evidence: ECO:0000255, ECO:0000269; organism taxon: NCBITaxon:243274. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Acetate kinase `UniProtKB:Q9WYB1`	located_in	cytoplasm `GO:0005737`	UniProtKB:Q9WYB1 Source assertion: UniProt locates UniProtKB:Q9WYB1 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000255. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`
Acetate kinase `UniProtKB:Q9WYB1`	has_cofactor	magnesium(2+) `CHEBI:18420`	UniProtKB:Q9WYB1 Source assertion: UniProt lists CHEBI:18420 as a cofactor of UniProtKB:Q9WYB1 (evidence: ECO:0000255, ECO:0000269). Mg(2+). Can also accept Mn(2+). Source location `comments[commentType='COFACTOR'].cofactors and note`
Acetate kinase `UniProtKB:Q9WYB1`	has_cofactor	manganese(2+) `CHEBI:29035`	UniProtKB:Q9WYB1 Source assertion: UniProt lists CHEBI:29035 as a cofactor of UniProtKB:Q9WYB1 (evidence: ECO:0000255, ECO:0000269). Mg(2+). Can also accept Mn(2+). Source location `comments[commentType='COFACTOR'].cofactors and note`
Phosphate acetyltransferase `UniProtKB:Q9X0L4`	enables	phosphate acetyltransferase reaction `RHEA:19522`	UniProtKB:Q9X0L4 Source assertion: UniProt annotates UniProtKB:Q9X0L4 with catalytic activity for the reaction family of RHEA:19522. Annotation evidence: curated source annotation; organism taxon: NCBITaxon:243274. Source location `comments[commentType='CATALYTIC ACTIVITY'].reaction.reactionCrossReferences`
Phosphate acetyltransferase `UniProtKB:Q9X0L4`	located_in	cytoplasm `GO:0005737`	UniProtKB:Q9X0L4 Source assertion: UniProt locates UniProtKB:Q9X0L4 in Cytoplasm (SL-0086, mapped by UniProt to GO:0005737). Annotation evidence: ECO:0000305. Source location `comments[commentType='SUBCELLULAR LOCATION'].subcellularLocations; UniProt location vocabulary GO mapping`

References

MetaCyc:PWY0-1312 MetaCyc acetate and ATP formation from acetyl-CoA I pathway (source version: 22.5)
RHEA:19521 Rhea phosphate acetyltransferase reaction (source version: 139)
RHEA:19522 Rhea left-to-right phosphate acetyltransferase direction (source version: 139)
RHEA:11352 Rhea acetate kinase reaction (source version: 139)
RHEA:11354 Rhea acetyl phosphate-to-acetate kinase direction (source version: 139)
PMID:10074080 Bock et al., Journal of Bacteriology, 1999
UniProtKB:Q9WYB1 UniProtKB:Q9WYB1 authority record (source version: 2026_03)
UniProtKB:Q9X0L4 UniProtKB:Q9X0L4 authority record (source version: 2026_03)

Curation history

2026-10-05T06:25:52Z codex
- EDIT — Review causal chemistry and add sourced molecular context
  Reviewed the entire record against MetaCyc 22.5 native pathway XML (including nested pathways), Rhea 139 explicit sides and directions, ENZYME 02-Sep-2026, PubMed records, and reviewed UniProt 2026_03 annotations within the record's taxon scope. Replaced generated structured-source quotations with source_assertion and source_locator. Typed molecular activities and participants. Reaction count 2 to 2; 0 directed-ID corrections; 2 protein annotations, 2 cofactor annotations and 2 sourced locations. All Rhea reaction substrate/product sets checked against independent Rhea authority; native pathway leaf coverage and source exceptions recorded in reports/causal_graph_review/metacyc-review.json. Full source URLs, versions and byte hashes are in metacyc-sources.json. All 50 MetaCyc records passed native schema validation; six targeted chemistry/evidence regression tests passed. Missing protein or cofactor annotations are not treated as biological absence; qualifications and exclusions are preserved in the ledger.
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