2-methylcitrate cycle I

MetaCyc:PWY0-42

Complete five-reaction MetaCyc route from propanoate activation through methylcitrate synthesis, dehydration, aconitase hydration and methylisocitrate cleavage to pyruvate and succinate, represented for Escherichia coli K-12 with primary experiments separately scoped to their strains and preparations. The central methylcitrate stereochemistry remains experimentally disputed. Approved stereounspecified Rhea reactions 57493 and 57497 and CHEBI:15598 preserve the established transformations without selecting an absolute stereoisomer or claiming all stereoisomers are substrates. These are broader reactions than the stereospecific native annotations, not exact replacements. PrpC and PrpB assemblies are explicit; AcnB monomer/dimer behavior is conditional. Acs and PrpE are alternative activation roles with different evidence strength. Oxaloacetate regeneration by the TCA cycle is outside the five-member native definition.

Pathway type
propionate catabolic pathway
Organism scope

Read the source YAML

Pathway network

30 nodes · 41 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Pathway network: 2-methylcitrate cycle I 30 nodes · 41 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. CHEBI:17272 — consumes → RHEA:20374; Evidence: RHEA:20374consumes CHEBI:30616 — consumes → RHEA:20374; Evidence: RHEA:20374consumes CHEBI:57287 — consumes → RHEA:20374; Evidence: RHEA:20374consumes RHEA:20374 — produces → CHEBI:57392; Evidence: RHEA:20374produces RHEA:20374 — produces → CHEBI:456215; Evidence: RHEA:20374produces RHEA:20374 — produces → CHEBI:33019; Evidence: RHEA:20374produces CHEBI:57392 — consumes → RHEA:57493; Evidence: RHEA:57493consumes CHEBI:16452 — consumes → RHEA:57493; Evidence: RHEA:57493consumes CHEBI:15377 — consumes → RHEA:57493; Evidence: RHEA:57493consumes RHEA:57493 — produces → CHEBI:15598; Stereounspecified source identity: this edge leaves methylcitrate absolute configuration unresolved and does not assert utilization or production of every stereoisomer.; Evidence: RHEA:57493produces RHEA:57493 — produces → CHEBI:57287; Evidence: RHEA:57493produces RHEA:57493 — produces → CHEBI:15378; Evidence: RHEA:57493produces CHEBI:15598 — consumes → RHEA:57497; Stereounspecified source identity: this edge leaves methylcitrate absolute configuration unresolved and does not assert utilization or production of every stereoisomer.; Evidence: RHEA:57497consumes RHEA:57497 — produces → CHEBI:57872; Evidence: RHEA:57497produces RHEA:57497 — produces → CHEBI:15377; Evidence: RHEA:57497produces CHEBI:57872 — consumes → RHEA:17943; Evidence: RHEA:17943consumes CHEBI:15377 — consumes → RHEA:17943; Evidence: RHEA:17943consumes RHEA:17943 — produces → CHEBI:57429; Evidence: RHEA:17943produces CHEBI:57429 — consumes → RHEA:16810; Evidence: RHEA:16810consumes RHEA:16810 — produces → CHEBI:15361; Evidence: RHEA:16810produces RHEA:16810 — produces → CHEBI:30031; Evidence: RHEA:16810produces RHEA:20374 — part_of → MetaCyc:PWY0-42; Evidence: MetaCyc:PWY0-42, MetaCyc:PROPIONATE--COA-LIGASE-RXN, RHEA:20374part of RHEA:57493 — part_of → MetaCyc:PWY0-42; The native reaction is stereospecific. This membership uses the separately issued generic parent reaction to represent the same supported pathway transformation while leaving the disputed methylcitrate configuration unspecified; no exactMatch is asserted.; Evidence: MetaCyc:PWY0-42, MetaCyc:2-METHYLCITRATE-SYNTHASE-RXN, RHEA:57493part of RHEA:57497 — part_of → MetaCyc:PWY0-42; The native reaction is stereospecific. This membership uses the separately issued generic parent reaction to represent the same supported pathway transformation while leaving the disputed methylcitrate configuration unspecified; no exactMatch is asserted.; Evidence: MetaCyc:PWY0-42, MetaCyc:2-METHYLCITRATE-DEHYDRATASE-RXN, RHEA:57497part of RHEA:17943 — part_of → MetaCyc:PWY0-42; Evidence: MetaCyc:PWY0-42, MetaCyc:4.2.1.99-RXN, RHEA:17943part of RHEA:16810 — part_of → MetaCyc:PWY0-42; Evidence: MetaCyc:PWY0-42, MetaCyc:METHYLISOCITRATE-LYASE-RXN, RHEA:16810part of RHEA:20374 — provides_input_for → RHEA:57493; Material transfer through CHEBI:57392. Ordering is not a thermodynamic irreversibility claim.; Evidence: MetaCyc:PWY0-42, RHEA:20374, RHEA:57493provides input for RHEA:57493 — provides_input_for → RHEA:57497; Material transfer through CHEBI:15598; the methylcitrate transfer retains an unspecified absolute configuration.; Evidence: MetaCyc:PWY0-42, RHEA:57493, RHEA:57497provides input for RHEA:57497 — provides_input_for → RHEA:17943; Material transfer through CHEBI:57872. Ordering is not a thermodynamic irreversibility claim.; Evidence: MetaCyc:PWY0-42, RHEA:57497, RHEA:17943provides input for RHEA:17943 — provides_input_for → RHEA:16810; Material transfer through CHEBI:57429. Ordering is not a thermodynamic irreversibility claim.; Evidence: MetaCyc:PWY0-42, RHEA:17943, RHEA:16810provides input for UniProtKB:P31660 — part_of → MetaCyc:CPLX0-251; Native assembly stoichiometry is 2 identical subunits; membership does not assert individual-subunit sufficiency.; Evidence: MetaCyc:CPLX0-251, MetaCyc:G6198-MONOMER, UniProtKB:P31660part of UniProtKB:P77541 — part_of → MetaCyc:CPLX0-1021; Native assembly stoichiometry is 4 identical subunits; membership does not assert individual-subunit sufficiency.; Evidence: MetaCyc:CPLX0-1021, MetaCyc:G6196-MONOMER, UniProtKB:P77541part of UniProtKB:P77495 — enables → RHEA:20374; Alternative annotated activation role. The E. coli reviewed FUNCTION is similarity-derived; the 2002 propionate-growth study favored Acs under its conditions. No essentiality or measured PrpE flux is asserted.; Evidence: UniProtKB:P77495, MetaCyc:G6200-MONOMERenables UniProtKB:P27550 — enables → RHEA:20374; Native curated propionate activation role plus condition-specific primary physiological inference. Current UniProt CATALYTIC ACTIVITY describes acetate activation, not this propionate equation; it is not used as direct support for substrate promiscuity or exclusive Acs dependence.; Evidence: MetaCyc:ACS-MONOMER, PMID:12473114enables MetaCyc:CPLX0-251 — catalyzes → RHEA:57493; The catalytic transformation is established; its exact methylcitrate stereochemical product is not selected. The source-issued generic reaction is used, not a modified RHEA:23781 equation.; Evidence: MetaCyc:CPLX0-251, UniProtKB:P31660, PMID:28956599, RHEA:57493catalyzes UniProtKB:P77243 — catalyzes → RHEA:57497; This records the physiological cis-product branch, not universal stereoisomer acceptance. Native curation preserves disagreement over whether PrpD contains an iron-sulfur cluster; one primary study reports an unstable [2Fe-2S] cluster in its preparation. No obligatory cofactor or cofactor-independence edge is asserted.; Evidence: UniProtKB:P77243, PMID:28956599, RHEA:57497, MetaCyc:G6199-MONOMER, PMID:11782506catalyzes UniProtKB:P36683 — enables → RHEA:17943; Functional gene-product role, without fixing a condition-independent oligomeric state. Native CPLX0-7761 has one component without a coefficient; current evidence supports conditional monomer/dimer behavior. The catabolic hydration is Rhea right-to-left relative to the master equation.; Evidence: UniProtKB:P36683, PMID:12473114, UniProtKB:P36683enables MetaCyc:CPLX0-1021 — catalyzes → RHEA:16810; Evidence: MetaCyc:CPLX0-1021, UniProtKB:P77541, PMID:11422389catalyzes UniProtKB:P27550 — has_cofactor → CHEBI:18420; Protein-level curated cofactor annotation; alternative-metal ranking is source-specific, not a newly measured propionate-specific metal series.; Evidence: UniProtKB:P27550has cofactor UniProtKB:P36683 — has_cofactor → CHEBI:49883; Catalytically active aconitase context; apo-AcnB RNA regulation and oxidative-stress effects are outside the chemical route.; Evidence: UniProtKB:P36683has cofactor MetaCyc:CPLX0-1021 — has_cofactor → CHEBI:18420; Evidence: UniProtKB:P77541, PMID:12706720has cofactor pyruvate; CHEBI:15361; Declared type: small_molecule small molecule pyruvate water; CHEBI:15377; Declared type: small_molecule small molecule water hydron; CHEBI:15378; Declared type: small_molecule small molecule hydron 2-methylcitrate(3-); CHEBI:15598; Declared type: small_molecule small molecule 2-methylcitrate(3-) oxaloacetate(2-); CHEBI:16452; Declared type: small_molecule small molecule oxaloacetate(2-) propionate; CHEBI:17272; Declared type: small_molecule small molecule propionate magnesium(2+); CHEBI:18420; Declared type: cofactor cofactor magnesium(2+) succinate(2-); CHEBI:30031; Declared type: small_molecule small molecule succinate(2-) ATP(4-); CHEBI:30616; Declared type: small_molecule small molecule ATP(4-) diphosphate(3-); CHEBI:33019; Declared type: small_molecule small molecule diphosphate(3-) adenosine 5'-monophosphate(2-); CHEBI:456215; Declared type: small_molecule small molecule adenosine 5'-monophosphate(2-) tetra-mu3-sulfido-tetrairon; CHEBI:49883; Declared type: cofactor cofactor tetra-mu3-sulfido-tetrairon coenzyme A(4-); CHEBI:57287; Declared type: small_molecule small molecule coenzyme A(4-) propionyl-CoA(4-); CHEBI:57392; Declared type: small_molecule small molecule propionyl-CoA(4-) (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate; CHEBI:57429; Declared type: small_molecule small molecule (2S,3R)-3-hydroxybutane- 1,2,3-tricarboxylate (Z)-but-2-ene-1,2,3-tricarboxylate; CHEBI:57872; Declared type: small_molecule small molecule (Z)-but-2-ene-1,2,3- tricarboxylate Propionate--CoA ligase; UniProtKB:P77495; Declared type: protein protein Propionate--CoA ligase Acetyl-coenzyme A synthetase; UniProtKB:P27550; Declared type: protein protein Acetyl-coenzyme A synthetase 2-methylcitrate synthase; UniProtKB:P31660; Declared type: protein protein 2-methylcitrate synthase 2-methylcitrate dehydratase; UniProtKB:P77243; Declared type: protein protein 2-methylcitrate dehydratase Aconitate hydratase B; UniProtKB:P36683; Declared type: protein protein Aconitate hydratase B 2-methylisocitrate lyase; UniProtKB:P77541; Declared type: protein protein 2-methylisocitrate lyase 2-methylcitrate synthase; MetaCyc:CPLX0-251; Declared type: complex complex 2-methylcitrate synthase 2-methylisocitrate lyase; MetaCyc:CPLX0-1021; Declared type: complex complex 2-methylisocitrate lyase propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate; RHEA:20374; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+); RHEA:57493; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+) 2-methylcitrate => 2-methyl-cis-aconitate + H2O; RHEA:57497; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity 2-methylcitrate => 2-methyl- cis-aconitate + H2O 2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate; RHEA:17943; Declared type: molecular_activity; Source reaction direction: right_to_left molecular activity 2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane- 1,2,3-tricarboxylate (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate => pyruvate + succinate; RHEA:16810; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity (2S,3R)-3-hydroxybutane- 1,2,3-tricarboxylate => pyruvate + succinate 2-methylcitrate cycle I; MetaCyc:PWY0-42; Declared type: pathway pathway 2-methylcitrate cycle I

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Escherichia coli str. K-12 substr. MG1655
NCBITaxon:511145		taxon
pyruvate
CHEBI:15361		small molecule
water
CHEBI:15377		small molecule
hydron
CHEBI:15378		small molecule
2-methylcitrate(3-)
CHEBI:15598		small molecule
oxaloacetate(2-)
CHEBI:16452		small molecule
propionate
CHEBI:17272		small molecule
magnesium(2+)
CHEBI:18420		cofactor
succinate(2-)
CHEBI:30031		small molecule
ATP(4-)
CHEBI:30616		small molecule
diphosphate(3-)
CHEBI:33019		small molecule
adenosine 5'-monophosphate(2-)
CHEBI:456215		small molecule
tetra-mu3-sulfido-tetrairon
CHEBI:49883		cofactor
coenzyme A(4-)
CHEBI:57287		small molecule
propionyl-CoA(4-)
CHEBI:57392		small molecule
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
CHEBI:57429		small molecule
(Z)-but-2-ene-1,2,3-tricarboxylate
CHEBI:57872		small molecule
Propionate--CoA ligase
UniProtKB:P77495		protein
Acetyl-coenzyme A synthetase
UniProtKB:P27550		protein
2-methylcitrate synthase
UniProtKB:P31660		protein
2-methylcitrate dehydratase
UniProtKB:P77243		protein
Aconitate hydratase B
UniProtKB:P36683		protein
2-methylisocitrate lyase
UniProtKB:P77541		protein
2-methylcitrate synthase
MetaCyc:CPLX0-251		complex
2-methylisocitrate lyase
MetaCyc:CPLX0-1021		complex
propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374		molecular activityleft to right
propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493		molecular activityleft to right
2-methylcitrate => 2-methyl-cis-aconitate + H2O
RHEA:57497		molecular activityleft to right
2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
RHEA:17943		molecular activityright to left
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate => pyruvate + succinate
RHEA:16810		molecular activityleft to right

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
propionate
CHEBI:17272		consumes

propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374
  • RHEA:20374

    Source assertion: RHEA:20374 assigns CHEBI:17272 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/20374']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/20373_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_20373_compound_4801']; rh:compound/rh:chebi
ATP(4-)
CHEBI:30616		consumes

propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374
  • RHEA:20374

    Source assertion: RHEA:20374 assigns CHEBI:30616 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/20374']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/20373_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_20373_compound_6372']; rh:compound/rh:chebi
coenzyme A(4-)
CHEBI:57287		consumes

propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374
  • RHEA:20374

    Source assertion: RHEA:20374 assigns CHEBI:57287 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/20374']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/20373_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_20373_compound_1264']; rh:compound/rh:chebi
propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374		produces

propionyl-CoA(4-)
CHEBI:57392
  • RHEA:20374

    Source assertion: RHEA:20374 assigns CHEBI:57392 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/20374']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/20373_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_20373_compound_3421']; rh:compound/rh:chebi
propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374		produces

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • RHEA:20374

    Source assertion: RHEA:20374 assigns CHEBI:456215 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/20374']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/20373_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_20373_compound_2590']; rh:compound/rh:chebi
propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374		produces

diphosphate(3-)
CHEBI:33019
  • RHEA:20374

    Source assertion: RHEA:20374 assigns CHEBI:33019 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/20374']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/20373_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_20373_compound_2106']; rh:compound/rh:chebi
propionyl-CoA(4-)
CHEBI:57392		consumes

propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493
  • RHEA:57493

    Source assertion: RHEA:57493 assigns CHEBI:57392 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57493']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57492_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57492_compound_3421']; rh:compound/rh:chebi
oxaloacetate(2-)
CHEBI:16452		consumes

propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493
  • RHEA:57493

    Source assertion: RHEA:57493 assigns CHEBI:16452 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57493']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57492_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57492_compound_5219']; rh:compound/rh:chebi
water
CHEBI:15377		consumes

propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493
  • RHEA:57493

    Source assertion: RHEA:57493 assigns CHEBI:15377 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57493']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57492_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57492_compound_1283']; rh:compound/rh:chebi
propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493		produces

Stereounspecified source identity: this edge leaves methylcitrate absolute configuration unresolved and does not assert utilization or production of every stereoisomer.

2-methylcitrate(3-)
CHEBI:15598
  • RHEA:57493

    Source assertion: RHEA:57493 assigns CHEBI:15598 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57493']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57492_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57492_compound_14908']; rh:compound/rh:chebi
propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493		produces

coenzyme A(4-)
CHEBI:57287
  • RHEA:57493

    Source assertion: RHEA:57493 assigns CHEBI:57287 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57493']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57492_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57492_compound_1264']; rh:compound/rh:chebi
propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493		produces

hydron
CHEBI:15378
  • RHEA:57493

    Source assertion: RHEA:57493 assigns CHEBI:15378 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57493']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57492_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57492_compound_3249']; rh:compound/rh:chebi
2-methylcitrate(3-)
CHEBI:15598		consumes

Stereounspecified source identity: this edge leaves methylcitrate absolute configuration unresolved and does not assert utilization or production of every stereoisomer.

2-methylcitrate => 2-methyl-cis-aconitate + H2O
RHEA:57497
  • RHEA:57497

    Source assertion: RHEA:57497 assigns CHEBI:15598 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57497']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57496_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57496_compound_14908']; rh:compound/rh:chebi
2-methylcitrate => 2-methyl-cis-aconitate + H2O
RHEA:57497		produces

(Z)-but-2-ene-1,2,3-tricarboxylate
CHEBI:57872
  • RHEA:57497

    Source assertion: RHEA:57497 assigns CHEBI:57872 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57497']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57496_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57496_compound_3416']; rh:compound/rh:chebi
2-methylcitrate => 2-methyl-cis-aconitate + H2O
RHEA:57497		produces

water
CHEBI:15377
  • RHEA:57497

    Source assertion: RHEA:57497 assigns CHEBI:15377 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57497']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57496_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57496_compound_1283']; rh:compound/rh:chebi
(Z)-but-2-ene-1,2,3-tricarboxylate
CHEBI:57872		consumes

2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
RHEA:17943
  • RHEA:17943

    Source assertion: RHEA:17943 assigns CHEBI:57872 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/17943']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/17941_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_17941_compound_3416']; rh:compound/rh:chebi
water
CHEBI:15377		consumes

2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
RHEA:17943
  • RHEA:17943

    Source assertion: RHEA:17943 assigns CHEBI:15377 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/17943']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/17941_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_17941_compound_1283']; rh:compound/rh:chebi
2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
RHEA:17943		produces

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
CHEBI:57429
  • RHEA:17943

    Source assertion: RHEA:17943 assigns CHEBI:57429 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/17943']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/17941_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_17941_compound_1381']; rh:compound/rh:chebi
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
CHEBI:57429		consumes

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate => pyruvate + succinate
RHEA:16810
  • RHEA:16810

    Source assertion: RHEA:16810 assigns CHEBI:57429 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/16810']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/16809_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_16809_compound_1381']; rh:compound/rh:chebi
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate => pyruvate + succinate
RHEA:16810		produces

pyruvate
CHEBI:15361
  • RHEA:16810

    Source assertion: RHEA:16810 assigns CHEBI:15361 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/16810']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/16809_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_16809_compound_3719']; rh:compound/rh:chebi
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate => pyruvate + succinate
RHEA:16810		produces

succinate(2-)
CHEBI:30031
  • RHEA:16810

    Source assertion: RHEA:16810 assigns CHEBI:30031 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/16810']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/16809_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_16809_compound_2501']; rh:compound/rh:chebi
propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374		part_of

MetaCyc:PWY0-42
  • MetaCyc:PWY0-42

    Source assertion: PWY0-42 includes PROPIONATE--COA-LIGASE-RXN in its five-member reaction-list.

    Source locationPathway[@frameid="PWY0-42"]/reaction-list/Reaction[@frameid="PROPIONATE--COA-LIGASE-RXN"]
  • MetaCyc:PROPIONATE--COA-LIGASE-RXN

    Source assertion: PROPIONATE--COA-LIGASE-RXN has native Rhea cross-reference 20373.

    Source locationReaction[@frameid="PROPIONATE--COA-LIGASE-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:20374

    Source assertion: Rhea master 20373 contains directed reaction 20374 and the native reaction cross-reference 20373 within its family.

    Source locationrdf:Description[@rdf:about="http://rdf.rhea-db.org/20373"]/rh:directionalReaction,rh:bidirectionalReaction
propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493		part_of

The native reaction is stereospecific. This membership uses the separately issued generic parent reaction to represent the same supported pathway transformation while leaving the disputed methylcitrate configuration unspecified; no exactMatch is asserted.

MetaCyc:PWY0-42
  • MetaCyc:PWY0-42

    Source assertion: PWY0-42 includes 2-METHYLCITRATE-SYNTHASE-RXN in its five-member reaction-list.

    Source locationPathway[@frameid="PWY0-42"]/reaction-list/Reaction[@frameid="2-METHYLCITRATE-SYNTHASE-RXN"]
  • MetaCyc:2-METHYLCITRATE-SYNTHASE-RXN

    Source assertion: 2-METHYLCITRATE-SYNTHASE-RXN has native Rhea cross-reference 23783.

    Source locationReaction[@frameid="2-METHYLCITRATE-SYNTHASE-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:57493

    Source assertion: Rhea master 23780 is a subclass of approved generic master 57492; the latter cites PMID:28956599 and supplies directed reaction 57493.

    Source locationrdf:Description[@rdf:about="http://rdf.rhea-db.org/23780"]/rdfs:subClassOf; rdf:Description[@rdf:about="http://rdf.rhea-db.org/57492"]/rh:status,rh:citation,rh:directionalReaction
2-methylcitrate => 2-methyl-cis-aconitate + H2O
RHEA:57497		part_of

The native reaction is stereospecific. This membership uses the separately issued generic parent reaction to represent the same supported pathway transformation while leaving the disputed methylcitrate configuration unspecified; no exactMatch is asserted.

MetaCyc:PWY0-42
  • MetaCyc:PWY0-42

    Source assertion: PWY0-42 includes 2-METHYLCITRATE-DEHYDRATASE-RXN in its five-member reaction-list.

    Source locationPathway[@frameid="PWY0-42"]/reaction-list/Reaction[@frameid="2-METHYLCITRATE-DEHYDRATASE-RXN"]
  • MetaCyc:2-METHYLCITRATE-DEHYDRATASE-RXN

    Source assertion: 2-METHYLCITRATE-DEHYDRATASE-RXN has native Rhea cross-reference 17728.

    Source locationReaction[@frameid="2-METHYLCITRATE-DEHYDRATASE-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:57497

    Source assertion: Rhea master 17725 is a subclass of approved generic master 57496; the latter cites PMID:28956599 and supplies directed reaction 57497.

    Source locationrdf:Description[@rdf:about="http://rdf.rhea-db.org/17725"]/rdfs:subClassOf; rdf:Description[@rdf:about="http://rdf.rhea-db.org/57496"]/rh:status,rh:citation,rh:directionalReaction
2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
RHEA:17943		part_of

MetaCyc:PWY0-42
  • MetaCyc:PWY0-42

    Source assertion: PWY0-42 includes 4.2.1.99-RXN in its five-member reaction-list.

    Source locationPathway[@frameid="PWY0-42"]/reaction-list/Reaction[@frameid="4.2.1.99-RXN"]
  • MetaCyc:4.2.1.99-RXN

    Source assertion: 4.2.1.99-RXN has native Rhea cross-reference 17944.

    Source locationReaction[@frameid="4.2.1.99-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:17943

    Source assertion: Rhea master 17941 contains directed reaction 17943 and the native reaction cross-reference 17944 within its family.

    Source locationrdf:Description[@rdf:about="http://rdf.rhea-db.org/17941"]/rh:directionalReaction,rh:bidirectionalReaction
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate => pyruvate + succinate
RHEA:16810		part_of

MetaCyc:PWY0-42
  • MetaCyc:PWY0-42

    Source assertion: PWY0-42 includes METHYLISOCITRATE-LYASE-RXN in its five-member reaction-list.

    Source locationPathway[@frameid="PWY0-42"]/reaction-list/Reaction[@frameid="METHYLISOCITRATE-LYASE-RXN"]
  • MetaCyc:METHYLISOCITRATE-LYASE-RXN

    Source assertion: METHYLISOCITRATE-LYASE-RXN has native Rhea cross-reference 16812.

    Source locationReaction[@frameid="METHYLISOCITRATE-LYASE-RXN"]/dblink[dblink-db="RHEA"]/dblink-oid
  • RHEA:16810

    Source assertion: Rhea master 16809 contains directed reaction 16810 and the native reaction cross-reference 16812 within its family.

    Source locationrdf:Description[@rdf:about="http://rdf.rhea-db.org/16809"]/rh:directionalReaction,rh:bidirectionalReaction
propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374		provides_input_for

Material transfer through CHEBI:57392. Ordering is not a thermodynamic irreversibility claim.

propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493
  • MetaCyc:PWY0-42

    Source assertion: The native ordering places PROPIONATE--COA-LIGASE-RXN immediately before 2-METHYLCITRATE-SYNTHASE-RXN.

    Source locationPathway[@frameid="PWY0-42"]/reaction-ordering[Reaction/@frameid="2-METHYLCITRATE-SYNTHASE-RXN"]/predecessor-reactions
  • RHEA:20374

    Source assertion: RHEA:20374 assigns CHEBI:57392 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/20374']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/20373_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_20373_compound_3421']; rh:compound/rh:chebi
  • RHEA:57493

    Source assertion: RHEA:57493 assigns CHEBI:57392 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57493']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57492_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57492_compound_3421']; rh:compound/rh:chebi
propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493		provides_input_for

Material transfer through CHEBI:15598; the methylcitrate transfer retains an unspecified absolute configuration.

2-methylcitrate => 2-methyl-cis-aconitate + H2O
RHEA:57497
  • MetaCyc:PWY0-42

    Source assertion: The native ordering places 2-METHYLCITRATE-SYNTHASE-RXN immediately before 2-METHYLCITRATE-DEHYDRATASE-RXN.

    Source locationPathway[@frameid="PWY0-42"]/reaction-ordering[Reaction/@frameid="2-METHYLCITRATE-DEHYDRATASE-RXN"]/predecessor-reactions
  • RHEA:57493

    Source assertion: RHEA:57493 assigns CHEBI:15598 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57493']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57492_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57492_compound_14908']; rh:compound/rh:chebi
  • RHEA:57497

    Source assertion: RHEA:57497 assigns CHEBI:15598 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57497']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57496_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57496_compound_14908']; rh:compound/rh:chebi
2-methylcitrate => 2-methyl-cis-aconitate + H2O
RHEA:57497		provides_input_for

Material transfer through CHEBI:57872. Ordering is not a thermodynamic irreversibility claim.

2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
RHEA:17943
  • MetaCyc:PWY0-42

    Source assertion: The native ordering places 2-METHYLCITRATE-DEHYDRATASE-RXN immediately before 4.2.1.99-RXN.

    Source locationPathway[@frameid="PWY0-42"]/reaction-ordering[Reaction/@frameid="4.2.1.99-RXN"]/predecessor-reactions
  • RHEA:57497

    Source assertion: RHEA:57497 assigns CHEBI:57872 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/57497']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/57496_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_57496_compound_3416']; rh:compound/rh:chebi
  • RHEA:17943

    Source assertion: RHEA:17943 assigns CHEBI:57872 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/17943']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/17941_R']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_17941_compound_3416']; rh:compound/rh:chebi
2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
RHEA:17943		provides_input_for

Material transfer through CHEBI:57429. Ordering is not a thermodynamic irreversibility claim.

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate => pyruvate + succinate
RHEA:16810
  • MetaCyc:PWY0-42

    Source assertion: The native ordering places 4.2.1.99-RXN immediately before METHYLISOCITRATE-LYASE-RXN.

    Source locationPathway[@frameid="PWY0-42"]/reaction-ordering[Reaction/@frameid="METHYLISOCITRATE-LYASE-RXN"]/predecessor-reactions
  • RHEA:17943

    Source assertion: RHEA:17943 assigns CHEBI:57429 to its products with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/17943']/rh:products; rdf:Description[@rdf:about='http://rdf.rhea-db.org/17941_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_17941_compound_1381']; rh:compound/rh:chebi
  • RHEA:16810

    Source assertion: RHEA:16810 assigns CHEBI:57429 to its substrates with coefficient 1.

    Source locationrdf:Description[@rdf:about='http://rdf.rhea-db.org/16810']/rh:substrates; rdf:Description[@rdf:about='http://rdf.rhea-db.org/16809_L']/rh:contains1[@rdf:resource='http://rdf.rhea-db.org/Participant_16809_compound_1381']; rh:compound/rh:chebi
2-methylcitrate synthase
UniProtKB:P31660		part_of

Native assembly stoichiometry is 2 identical subunits; membership does not assert individual-subunit sufficiency.

2-methylcitrate synthase
MetaCyc:CPLX0-251
  • MetaCyc:CPLX0-251

    Source assertion: CPLX0-251 contains 2 copies of G6198-MONOMER.

    Source locationProtein[@frameid="CPLX0-251"]/component/Protein; component/coefficient
  • MetaCyc:G6198-MONOMER

    Source assertion: G6198-MONOMER has UniProt cross-reference P31660.

    Source locationProtein[@frameid="G6198-MONOMER"]/dblink[dblink-db="UNIPROT"]/dblink-oid
  • UniProtKB:P31660

    Source assertion: The reviewed SUBUNIT annotation states homodimer (ECO:0000269; PMID:9579066).

    Source location$.comments[?(@.commentType=="SUBUNIT")]
2-methylisocitrate lyase
UniProtKB:P77541		part_of

Native assembly stoichiometry is 4 identical subunits; membership does not assert individual-subunit sufficiency.

2-methylisocitrate lyase
MetaCyc:CPLX0-1021
  • MetaCyc:CPLX0-1021

    Source assertion: CPLX0-1021 contains 4 copies of G6196-MONOMER.

    Source locationProtein[@frameid="CPLX0-1021"]/component/Protein; component/coefficient
  • MetaCyc:G6196-MONOMER

    Source assertion: G6196-MONOMER has UniProt cross-reference P77541.

    Source locationProtein[@frameid="G6196-MONOMER"]/dblink[dblink-db="UNIPROT"]/dblink-oid
  • UniProtKB:P77541

    Source assertion: The reviewed SUBUNIT annotation states homotetramer, a dimer of dimers (ECO:0000269; PMID:12706720 and PMID:15723538; also HAMAP ECO:0000255).

    Source location$.comments[?(@.commentType=="SUBUNIT")]
Propionate--CoA ligase
UniProtKB:P77495		enables

Alternative annotated activation role. The E. coli reviewed FUNCTION is similarity-derived; the 2002 propionate-growth study favored Acs under its conditions. No essentiality or measured PrpE flux is asserted.

propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374
  • UniProtKB:P77495

    Source assertion: FUNCTION assigns synthesis of propionyl-CoA from propionate and CoA by similarity (ECO:0000250); CATALYTIC ACTIVITY cross-references RHEA:20373.

    Source location$.comments[0:2]
  • MetaCyc:G6200-MONOMER

    Source assertion: G6200-MONOMER maps to P77495 and catalyzes PROPIONATE--COA-LIGASE-RXN through ENZRXN0-7593.

    Source locationProtein[@frameid="G6200-MONOMER"]/dblink[dblink-db="UNIPROT"]; catalyzes/Enzymatic-Reaction[@frameid="ENZRXN0-7593"]/reaction
Acetyl-coenzyme A synthetase
UniProtKB:P27550		enables

Native curated propionate activation role plus condition-specific primary physiological inference. Current UniProt CATALYTIC ACTIVITY describes acetate activation, not this propionate equation; it is not used as direct support for substrate promiscuity or exclusive Acs dependence.

propanoate + ATP + CoA => propanoyl-CoA + AMP + diphosphate
RHEA:20374
  • MetaCyc:ACS-MONOMER

    Source assertion: ACS-MONOMER maps to P27550 and catalyzes PROPIONATE--COA-LIGASE-RXN through ENZRXN0-1507.

    Source locationProtein[@frameid="ACS-MONOMER"]/dblink[dblink-db="UNIPROT"]; catalyzes/Enzymatic-Reaction[@frameid="ENZRXN0-1507"]/reaction
  • PMID:12473114

    Source assertion: In propionate-grown E. coli, the abstract reports detection of prp-operon proteins except PrpE and proposes its replacement by acetyl-CoA synthetase.

    Source location$.resultList.result[0].abstractText; final two sentences
2-methylcitrate synthase
MetaCyc:CPLX0-251		catalyzes

The catalytic transformation is established; its exact methylcitrate stereochemical product is not selected. The source-issued generic reaction is used, not a modified RHEA:23781 equation.

propanoyl-CoA + oxaloacetate + H2O => 2-methylcitrate + CoA + H(+)
RHEA:57493
  • MetaCyc:CPLX0-251

    Source assertion: The PrpC complex catalyzes 2-METHYLCITRATE-SYNTHASE-RXN through ENZRXN0-245.

    Source locationProtein[@frameid="CPLX0-251"]/catalyzes/Enzymatic-Reaction[@frameid="ENZRXN0-245"]/reaction
  • UniProtKB:P31660

    Source assertion: FUNCTION assigns condensation of propionyl-CoA and oxaloacetate to methylcitrate and CoA (ECO:0000269). CAUTION preserves conflicting absolute stereochemistry assignments from PMID:12473114 and PMID:28956599.

    Source location$.comments[0]; $.comments[9]
  • PMID:28956599

    Source assertion: The abstract reports that E. coli PrpC and B. subtilis MmgD generate the same methylcitrate stereoisomer, assigned alternatively as (2S,3R) or (2R,3S).

    Source location$.resultList.result[0].abstractText
  • RHEA:57493

    Source assertion: Approved generic synthesis reaction 57493 belongs to master 57492, which cites PMID:28956599 and is the parent of specific master 23780.

    Source locationrdf:Description[@rdf:about="http://rdf.rhea-db.org/57493"]; rdf:Description[@rdf:about="http://rdf.rhea-db.org/23780"]/rdfs:subClassOf
2-methylcitrate dehydratase
UniProtKB:P77243		catalyzes

This records the physiological cis-product branch, not universal stereoisomer acceptance. Native curation preserves disagreement over whether PrpD contains an iron-sulfur cluster; one primary study reports an unstable [2Fe-2S] cluster in its preparation. No obligatory cofactor or cofactor-independence edge is asserted.

2-methylcitrate => 2-methyl-cis-aconitate + H2O
RHEA:57497
  • UniProtKB:P77243

    Source assertion: FUNCTION assigns dehydration of methylcitrate to cis-methylaconitate (ECO:0000269; PMID:11782506 and PMID:12473114); SUBUNIT states monomer. CAUTION retains the substrate stereochemistry conflict.

    Source location$.comments[0]; $.comments[6]; $.comments[11]
  • PMID:28956599

    Source assertion: The abstract reports that E. coli PrpD can dehydrate at least two methylcitrate stereoisomers; the physiological pathways proceed through (Z)-2-methylaconitate.

    Source location$.resultList.result[0].abstractText
  • RHEA:57497

    Source assertion: Approved generic dehydration 57497 belongs to master 57496, which cites PMID:28956599 and is the parent of specific master 17725.

    Source locationrdf:Description[@rdf:about="http://rdf.rhea-db.org/57497"]; rdf:Description[@rdf:about="http://rdf.rhea-db.org/17725"]/rdfs:subClassOf
  • MetaCyc:G6199-MONOMER

    Source assertion: The native comment preserves disagreement between PMID:11782506 and PMID:12473114 over whether PrpD contains an iron-sulfur cluster. This is a curated conflict statement, not a resolution of the metal requirement.

    Source locationProtein[@frameid="G6199-MONOMER"]/comment
  • PMID:11782506

    Source assertion: The primary abstract reports one unstable [2Fe-2S] cluster per purified PrpD monomer, needed for maximum catalytic activity in that preparation and reconstitutable with Fe(2+) under reducing conditions.

    Source location$.resultList.result[0].abstractText
Aconitate hydratase B
UniProtKB:P36683		enables

Functional gene-product role, without fixing a condition-independent oligomeric state. Native CPLX0-7761 has one component without a coefficient; current evidence supports conditional monomer/dimer behavior. The catabolic hydration is Rhea right-to-left relative to the master equation.

2-methyl-cis-aconitate + H2O => (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
RHEA:17943
  • UniProtKB:P36683

    Source assertion: FUNCTION assigns hydration of cis-2-methylaconitate to (2R,3S)-2-methylisocitrate; CATALYTIC ACTIVITY cites RHEA:17941 with ECO:0000269, PMID:12473114.

    Source location$.comments[0]; $.comments[2]
  • PMID:12473114

    Source assertion: AcnB was purified from propionate-grown E. coli, identified by its N-terminus, and required with PrpD for conversion of methylcitrate to methylisocitrate.

    Source location$.resultList.result[0].abstractText
  • UniProtKB:P36683

    Source assertion: SUBUNIT describes monomer and possible homodimer with iron-dependent interconversion (ECO:0000269).

    Source location$.comments[7]
2-methylisocitrate lyase
MetaCyc:CPLX0-1021		catalyzes

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate => pyruvate + succinate
RHEA:16810
  • MetaCyc:CPLX0-1021

    Source assertion: The PrpB complex catalyzes METHYLISOCITRATE-LYASE-RXN through ENZRXN0-1521.

    Source locationProtein[@frameid="CPLX0-1021"]/catalyzes/Enzymatic-Reaction[@frameid="ENZRXN0-1521"]/reaction
  • UniProtKB:P77541

    Source assertion: CATALYTIC ACTIVITY assigns methylisocitrate cleavage to pyruvate and succinate, RHEA:16809 (ECO:0000269; PMID:11422389 and PMID:15723538; also HAMAP ECO:0000255).

    Source location$.comments[1]
  • PMID:11422389

    Source assertion: The purified E. coli PrpB is homotetrameric by size-exclusion chromatography and cleaves threo-methylisocitrate; the erythro diastereomer was inactive.

    Source location$.resultList.result[0].abstractText
Acetyl-coenzyme A synthetase
UniProtKB:P27550		has_cofactor

Protein-level curated cofactor annotation; alternative-metal ranking is source-specific, not a newly measured propionate-specific metal series.

magnesium(2+)
CHEBI:18420
  • UniProtKB:P27550

    Source assertion: COFACTOR assigns Mg2+ with ECO:0000269 (PMID:15327942 and PMID:21941) and HAMAP ECO:0000255; other divalent metals support decreasing activity in the order Mg, Mn, Ca, Ni.

    Source location$.comments[3]
Aconitate hydratase B
UniProtKB:P36683		has_cofactor

Catalytically active aconitase context; apo-AcnB RNA regulation and oxidative-stress effects are outside the chemical route.

tetra-mu3-sulfido-tetrairon
CHEBI:49883
  • UniProtKB:P36683

    Source assertion: COFACTOR assigns one [4Fe-4S] cluster per subunit, CHEBI:49883 (ECO:0000269; PMID:11992126).

    Source location$.comments[3]
2-methylisocitrate lyase
MetaCyc:CPLX0-1021		has_cofactor

magnesium(2+)
CHEBI:18420
  • UniProtKB:P77541

    Source assertion: COFACTOR assigns Mg2+, CHEBI:18420, with experimental ECO:0000269 references PMID:11422389, PMID:12706720 and PMID:15723538, plus HAMAP ECO:0000255.

    Source location$.comments[2]
  • PMID:12706720

    Source assertion: The primary abstract states that E. coli PrpB strictly depends on Mg2+ for catalysis.

    Source location$.resultList.result[0].abstractText

References

Curation history