fructoselysine and psicoselysine degradation

MetaCyc:PWY0-521

The source-defined three-reaction pathway converts psicoselysine to fructoselysine through FrlC, then uses ATP-dependent FrlD phosphorylation and FrlB deglycation to produce glucose 6-phosphate and lysine. Fructoselysine enters at the kinase step. The two downstream reactions overlap the narrower GO:1901281 fructoselysine catabolic record; these are overlapping boundaries, not disjoint capabilities or exact identifier synonyms. The graph selects catabolic traversal while preserving FrlC reversibility and in vitro FrlB reversibility. Native MG1655 annotations, reviewed K-12 protein identities, experimental constructs and conditional growth evidence retain separate scopes.

Pathway type
glycated amino acid catabolism
Organism scope

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Pathway network

41 nodes · 35 asserted directed edges

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Pathway network: fructoselysine and psicoselysine degradation 41 nodes · 35 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. MetaCyc:PWY0-521 — has_part → RHEA:28391; Native core membership; exact chemistry and the selected directional Rhea member are independently checked. Native RXN0-4841 is reversible with opposite printed orientation; its R2L pathway layout equals this Rhea L2R psicoselysine entry.; Evidence: MetaCyc:PWY0-521, MetaCyc:RXN0-4841, RHEA:28391has part CHEBI:61403 — consumes → RHEA:28391; Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.; Evidence: RHEA:28391consumes RHEA:28391 — produces → CHEBI:61393; Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.; Evidence: RHEA:28391produces MetaCyc:PWY0-521 — has_part → RHEA:28379; Native core membership; exact chemistry and the selected directional Rhea member are independently checked.; Evidence: MetaCyc:PWY0-521, MetaCyc:RXN0-962, RHEA:28379has part CHEBI:61393 — consumes → RHEA:28379; Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.; Evidence: RHEA:28379consumes CHEBI:30616 — consumes → RHEA:28379; Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.; Evidence: RHEA:28379consumes RHEA:28379 — produces → CHEBI:61392; Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.; Evidence: RHEA:28379produces RHEA:28379 — produces → CHEBI:456216; Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.; Evidence: RHEA:28379produces RHEA:28379 — produces → CHEBI:15378; Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.; Evidence: RHEA:28379produces MetaCyc:PWY0-521 — has_part → MetaCyc:RXN0-963; Native core membership preserves the beta-D-glucose 6-phosphate product; the source Rhea cross-reference has broader anomer scope and is not treated as exact. This catabolic direction does not assert thermodynamic irreversibility; FrlB also catalyzes the reverse conversion in vitro.; Evidence: MetaCyc:PWY0-521, MetaCyc:RXN0-963has part CHEBI:61392 — consumes → MetaCyc:RXN0-963; Complete native deglycase side; exact beta-D-glucose 6-phosphate chemistry is retained. This stereochemical scope is a native database assertion, not automatically an independently measured primary-study product.; Evidence: MetaCyc:RXN0-963, MetaCyc:FRUCTOSELYSINE-6-PHOSPHATEconsumes CHEBI:15377 — consumes → MetaCyc:RXN0-963; Complete native deglycase side; exact beta-D-glucose 6-phosphate chemistry is retained. This stereochemical scope is a native database assertion, not automatically an independently measured primary-study product.; Evidence: MetaCyc:RXN0-963, MetaCyc:WATERconsumes MetaCyc:RXN0-963 — produces → CHEBI:58247; Complete native deglycase side; exact beta-D-glucose 6-phosphate chemistry is retained. This stereochemical scope is a native database assertion, not automatically an independently measured primary-study product.; Evidence: MetaCyc:RXN0-963, MetaCyc:GLC-6-Pproduces MetaCyc:RXN0-963 — produces → CHEBI:32551; Complete native deglycase side; exact beta-D-glucose 6-phosphate chemistry is retained. This stereochemical scope is a native database assertion, not automatically an independently measured primary-study product.; Evidence: MetaCyc:RXN0-963, MetaCyc:LYSproduces RHEA:28391 — provides_input_for → RHEA:28379; The selected upstream event provides fructoselysine to the next reaction; fructoselysine may also enter directly at the kinase.; Evidence: MetaCyc:PWY0-521provides input for RHEA:28379 — provides_input_for → MetaCyc:RXN0-963; The selected upstream event provides fructoselysine 6-phosphate to the next reaction; fructoselysine may also enter directly at the kinase.; Evidence: MetaCyc:PWY0-521provides input for MetaCyc:CPLX0-3861 — has_part → UniProtKB:P45541; Native homomer annotation contains 8 copies of the linked polypeptide. Source assembly inference does not establish a solved structure or exact experimental construct.; Evidence: MetaCyc:CPLX0-3861, MetaCyc:G7724-MONOMERhas part MetaCyc:CPLX0-821 — has_part → UniProtKB:P0AC00; Native homomer annotation contains 12 copies of the linked polypeptide. Source assembly inference does not establish a solved structure or exact experimental construct.; Evidence: MetaCyc:CPLX0-821, MetaCyc:G7723-MONOMERhas part MetaCyc:CPLX0-3861 — catalyzes → RHEA:28391; Curated native catalytic assignment; this annotation is separate from experimental preparation claims.; Evidence: MetaCyc:CPLX0-3861, MetaCyc:RXN0-4841, RHEA:28391catalyzes UniProtKB:P45543 — catalyzes → RHEA:28379; Curated native catalytic assignment; this annotation is separate from experimental preparation claims.; Evidence: MetaCyc:G7726-MONOMER, MetaCyc:G7726-MONOMER, MetaCyc:RXN0-962, RHEA:28379catalyzes MetaCyc:CPLX0-821 — catalyzes → MetaCyc:RXN0-963; Curated native catalytic assignment; this annotation is separate from experimental preparation claims.; Evidence: MetaCyc:CPLX0-821, MetaCyc:RXN0-963catalyzes MetaCyc:CPLX0-3861 — catalyzes → RHEA:28391; subject experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value); Purified recombinant FrlC interconverts the two substrates in vitro; Figure 4 includes both starting substrates and enzyme-free controls. The selected graph orientation follows psicoselysine catabolism.; Evidence: PMID:14641112catalyzes MetaCyc:CPLX0-3861 — has_cofactor → CHEBI:49786; subject experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value); Alternative in vitro metal support; nickel and cobalt enhance activity more than iron or manganese. The extract assay includes 1 mM MgCl2. Physiological occupancy, fixed stoichiometry and simultaneous binding of these alternatives are not established.; Evidence: PMID:14641112, UniProtKB:P45541has cofactor MetaCyc:CPLX0-3861 — has_cofactor → CHEBI:48828; subject experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value); Alternative in vitro metal support; nickel and cobalt enhance activity more than iron or manganese. The extract assay includes 1 mM MgCl2. Physiological occupancy, fixed stoichiometry and simultaneous binding of these alternatives are not established.; Evidence: PMID:14641112, UniProtKB:P45541has cofactor MetaCyc:CPLX0-3861 — has_cofactor → CHEBI:29033; subject experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value); Alternative in vitro metal support; nickel and cobalt enhance activity more than iron or manganese. The extract assay includes 1 mM MgCl2. Physiological occupancy, fixed stoichiometry and simultaneous binding of these alternatives are not established.; Evidence: PMID:14641112, UniProtKB:P45541has cofactor MetaCyc:CPLX0-3861 — has_cofactor → CHEBI:29035; subject experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value); Alternative in vitro metal support; nickel and cobalt enhance activity more than iron or manganese. The extract assay includes 1 mM MgCl2. Physiological occupancy, fixed stoichiometry and simultaneous binding of these alternatives are not established.; Evidence: PMID:14641112, UniProtKB:P45541has cofactor CHEBI:29105 — inhibits → MetaCyc:CPLX0-3861; object experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value); In vitro inhibition in the recombinant extract assay: 10 micromolar Zn2+ completely inhibits reported activity, with underlying results not shown. No physiological regulatory role is inferred.; Evidence: PMID:14641112inhibits UniProtKB:P45539 — enables → MetaCyc:PWY0-521; subject experimental construct: Indirect gene-product attribution from the FB21138 frlA Tn5 growth comparison at 20 mM substrates. The Materials passage does not specify this mutant background; the separately described BL21 cultures do not establish it. No complementation or purified FrlA transport assay is reported.; Evidence PMID:14641112: FB21138 is a Blattner-collection frlA Tn5 mutant; growth was followed in M9 with 20 mM substrates. The reported phenotype lacks a rescue experiment. (p1047 Materials; p1048 Growth of E. coli on psicoselysine; p1051 growth Results); FrlA participation is inferred from the growth phenotype and reviewed probable-transporter annotation. Tn5 polarity and lack of rescue limit gene-product attribution; transport itself, coupling and stoichiometry were not measured. FrlA is context, not a core reaction member.; Evidence: PMID:14641112, UniProtKB:P45539enables UniProtKB:P45543 — catalyzes → RHEA:28379; subject experimental construct: FrlD from BL21(DE3) genomic DNA in pET-3a, expressed in BL21(DE3)pLysS; no affinity tag stated. The start-primer sequence matches current K-12 P45543, but complete experimental sequence identity is not established. Approximately 28-kDa gel-filtration behavior supports the monomer assignment.; Evidence PMID:12147680: Methods describe the BL21(DE3) donor and pET-3a expression in BL21(DE3)pLysS; Results assigns monomeric behavior from gel filtration. (p42524 Overexpression and purification of fructoselysine 6-kinase; p42526 Expression and characterization; Fig5 caption); Recombinant FrlD catalyzes ATP-dependent C6 phosphorylation. Product NMR establishes phosphate position while retaining multiple solution forms at pH 4.1. Mg salts are assay conditions; their inclusion alone does not establish an indispensable cofactor. NADH and phosphoenolpyruvate belong to coupled detection.; Evidence: PMID:12147680catalyzes MetaCyc:CPLX0-821 — catalyzes → MetaCyc:RXN0-963; subject experimental construct: Active first-AUG FrlB from BL21(DE3) DNA, pET-3a expression in BL21(DE3)pLysS. Its start primer matches current P0AC00 residues 1 onward; complete donor sequence identity is unproved. The second-AUG construct was insoluble/inactive. Approximately 450-kDa gel filtration suggests a dodecamer; the assembly trace was not shown.; Evidence PMID:12147680: The first-AUG construct is active, whereas the second-AUG product is insoluble with no detected activity. An approximately 450-kDa apparent mass suggests a dodecamer. (p42524 Overexpression and purification of fructoselysine-6-phosphate deglycase; p42527 Expression and characterization of putative deglycase); Recombinant FrlB supports reversible cleavage to lysine and glucose 6-phosphate, including the reverse condensation experiment. The enzymatic glucose-6-phosphate identification does not establish anomer specificity: the beta product attached to this native reaction remains a separate database assertion.; Evidence: PMID:12147680, MetaCyc:GLC-6-Pcatalyzes CHEBI:29105 — inhibits → MetaCyc:CPLX0-821; object experimental construct: Active first-AUG FrlB from BL21(DE3) DNA, pET-3a expression in BL21(DE3)pLysS. Its start primer matches current P0AC00 residues 1 onward; complete donor sequence identity is unproved. The second-AUG construct was insoluble/inactive. Approximately 450-kDa gel filtration suggests a dodecamer; the assembly trace was not shown.; Evidence PMID:12147680: The first-AUG construct is active, whereas the second-AUG product is insoluble with no detected activity. An approximately 450-kDa apparent mass suggests a dodecamer. (p42524 Overexpression and purification of fructoselysine-6-phosphate deglycase; p42527 Expression and characterization of putative deglycase); In vitro inhibition: 10 micromolar ZnCl2 completely suppresses the recombinant preparation activity. EGTA/EDTA stimulated activity and later assays included 0.1 mM EGTA; the zinc-test buffer is not separately fully restated. This is not physiological zinc regulation.; Evidence: PMID:12147680inhibits UniProtKB:P45544 — inhibits → MetaCyc:PWY0-521; subject experimental construct: E. coli FrlR expressed from pBAD33 in BW25113/JW5698 PfrlABCD-lux reporter comparisons. Exact construction/primer tables were unavailable; full insert identity to current K-12 P45544 is not independently established. The measured target is a shared-promoter reporter, not each endogenous transcript or an MG1655 gene instance.; Evidence PMID:32979851: BW25113 and frlR-deleted JW5698 lux reporters are compared with ectopic pBAD33-FrlR; construction details are referred to supporting Table S2. (Thesis PDF pp89–90 (article pp5–6), Figs5b/6; Methods4.5 article p13 / PDF97); Transcriptional repression of the shared frlABCD promoter controls pathway expression in the BW25113 reporter system. This process-level context edge does not assert direct inhibition of the pathway enzymes, separate endogenous transcript measurements, regulation of frlR itself, or psicoselysine-specific reporter testing.; Evidence: PMID:32979851inhibits UniProtKB:P45544 — regulates → MetaCyc:PWY0-521; subject experimental construct: Separate in vitro preparation: His6-SUMO-FrlR expressed in BL21(DE3), cleaved with His6-Ulp1 and recovered after metal-affinity cleanup. Exact supplementary construct sequence is unverified. Operator DNA is the assay target; the reference protein ID does not establish a particular MG1655 promoter or full-length native dimer.; Evidence PMID:32979851: Protein purification uses His6-SUMO expression in BL21(DE3), His6-Ulp1 cleavage and recovery of tag-free FrlR; SPR compares operator-bearing and control DNA. (Thesis PDF pp97–98 (article pp13–14), Methods4.6–4.7); Operator binding supplies the molecular basis for the separately observed transcriptional repression. SPR measured operator-dependent binding, with reported KD 4.9 nM after active-concentration correction; engineered T7/operator reporters support sequence dependence. This is regulatory context, not a new metabolic event.; Evidence: PMID:32979851regulates UniProtKB:P0AGB3 — activates → MetaCyc:PWY0-521; subject experimental construct: pBAD33-rpoH overexpression in BW25113 shared-promoter lux reporter assays, with promoter truncations and temperature conditions specified by Figure 5c and Methods4.5. Exact supplementary insert sequence was not inspected; current K-12 RpoH denotes gene-product identity.; Evidence PMID:32979851: The reporter experiment overproduces RpoH from pBAD33; Methods specifies adjusted measurement temperatures for rpoH analysis. (Thesis PDF pp89–90 Fig5c and Results2.3; PDF97 Methods4.5); Positive transcriptional context from RpoH overexpression and promoter-truncation reporters. This is not a purified sigma-factor binding experiment, direct enzyme activation, individual endogenous-transcript measurement, or a host-adaptation experiment.; Evidence: PMID:32979851activates UniProtKB:P0ACJ8 — activates → MetaCyc:PWY0-521; subject experimental construct: Indirect CRP attribution in BW25113/JW3778 cyaA and PfrlABCD-lux reporter comparisons. CRP itself was neither deleted nor purified in these experiments; promoter DNA and reporter context were engineered. Exact equivalence of every tested sequence to the reference K-12 molecules is not established.; Evidence PMID:32979851: The cAMP reporter comparisons use cyaA-deleted JW3778, ectopic cyaA and truncated promoter fusions; the CRP-directed interpretation is indirect. (Thesis PDF pp89–90 Fig5d and Results2.3; Methods4.5 PDF97); Inferred CRP/cAMP-dependent positive transcriptional control from cyaA manipulation, glucose response and candidate-CRP-region truncation. The study supplies no new purified CRP binding or crp-deletion experiment. The source differs between 10 and 20 mM glucose descriptions; no single dose is selected here.; Evidence: PMID:32979851activates psicosyllysine(1+); CHEBI:61403; Declared type: small_molecule small molecule psicosyllysine(1+) fructosyllysine(1+); CHEBI:61393; Declared type: small_molecule small molecule fructosyllysine(1+) ATP(4-); CHEBI:30616; Declared type: small_molecule small molecule ATP(4-) fructoselysine 6-phosphate(1-); CHEBI:61392; Declared type: small_molecule small molecule fructoselysine 6-phosphate(1-) ADP(3-); CHEBI:456216; Declared type: small_molecule small molecule ADP(3-) hydron; CHEBI:15378; Declared type: small_molecule small molecule hydron water; CHEBI:15377; Declared type: small_molecule small molecule water beta-D-glucose 6-phosphate(2-); CHEBI:58247; Declared type: small_molecule small molecule beta-D-glucose 6-phosphate(2-) L-lysinium(1+); CHEBI:32551; Declared type: small_molecule small molecule L-lysinium(1+) nickel(2+); CHEBI:49786; Declared type: cofactor cofactor nickel(2+) cobalt(2+); CHEBI:48828; Declared type: cofactor cofactor cobalt(2+) iron(2+); CHEBI:29033; Declared type: cofactor cofactor iron(2+) manganese(2+); CHEBI:29035; Declared type: cofactor cofactor manganese(2+) zinc(2+); CHEBI:29105; Declared type: small_molecule small molecule zinc(2+) Fructoselysine 3-epimerase; UniProtKB:P45541; Declared type: protein protein Fructoselysine 3-epimerase Fructoselysine 6-kinase; UniProtKB:P45543; Declared type: protein protein Fructoselysine 6-kinase Fructoselysine 6-phosphate deglycase; UniProtKB:P0AC00; Declared type: protein protein Fructoselysine 6-phosphate deglycase Probable fructoselysine/psicoselysine transporter FrlA; UniProtKB:P45539; Declared type: protein protein Probable fructoselysine/psicoselysine transporter FrlA Probable fructoselysine utilization operon transcriptional repressor; UniProtKB:P45544; Declared type: protein protein Probable fructoselysine utilization operon transcriptional repressor RNA polymerase sigma factor RpoH; UniProtKB:P0AGB3; Declared type: protein protein RNA polymerase sigma factor RpoH DNA-binding transcriptional dual regulator CRP; UniProtKB:P0ACJ8; Declared type: protein protein DNA-binding transcriptional dual regulator CRP fructoselysine 3-epimerase; MetaCyc:CPLX0-3861; Declared type: complex complex fructoselysine 3-epimerase fructoselysine 6-phosphate deglycase; MetaCyc:CPLX0-821; Declared type: complex complex fructoselysine 6-phosphate deglycase N(6)-(D-psicosyl)-L-lysine => N(6)-(D-fructosyl)-L-lysine; RHEA:28391; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity N(6)-(D-psicosyl)-L-lysine => N(6)-(D-fructosyl)-L-lysine N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+); RHEA:28379; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D- fructosyl)-L-lysine + ADP + H(+) fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine; MetaCyc:RXN0-963; Declared type: molecular_activity; Source reaction direction: left_to_right molecular activity fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine fructoselysine and psicoselysine degradation; MetaCyc:PWY0-521; Declared type: pathway pathway fructoselysine and psicoselysine degradation fructoselysine 3-epimerase; MetaCyc:CPLX0-3861; Declared type: complex; experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value) experimental construct fructoselysine 3-epimerase [experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region…] fructoselysine 3-epimerase; MetaCyc:CPLX0-3861; Declared type: complex; experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value) experimental construct fructoselysine 3-epimerase [experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region…] fructoselysine 3-epimerase; MetaCyc:CPLX0-3861; Declared type: complex; experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value) experimental construct fructoselysine 3-epimerase [experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region…] fructoselysine 3-epimerase; MetaCyc:CPLX0-3861; Declared type: complex; experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value) experimental construct fructoselysine 3-epimerase [experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region…] fructoselysine 3-epimerase; MetaCyc:CPLX0-3861; Declared type: complex; experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value) experimental construct fructoselysine 3-epimerase [experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region…] fructoselysine 3-epimerase; MetaCyc:CPLX0-3861; Declared type: complex; experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.; Evidence PMID:14641112: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer. (p1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization); Evidence UniProtKB:P45541: The current reference sequence contains 276 residues, with terminal arginine. (primaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value) experimental construct fructoselysine 3-epimerase [experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region…] Probable fructoselysine/psicoselysine transporter FrlA; UniProtKB:P45539; Declared type: protein; experimental construct: Indirect gene-product attribution from the FB21138 frlA Tn5 growth comparison at 20 mM substrates. The Materials passage does not specify this mutant background; the separately described BL21 cultures do not establish it. No complementation or purified FrlA transport assay is reported.; Evidence PMID:14641112: FB21138 is a Blattner-collection frlA Tn5 mutant; growth was followed in M9 with 20 mM substrates. The reported phenotype lacks a rescue experiment. (p1047 Materials; p1048 Growth of E. coli on psicoselysine; p1051 growth Results) experimental construct Probable fructoselysine/psicoselysine transporter FrlA [experimental construct: Indirect gene-product attribution from the FB21138 frlA Tn5 growth comparison at 20 mM substrates.…] Fructoselysine 6-kinase; UniProtKB:P45543; Declared type: protein; experimental construct: FrlD from BL21(DE3) genomic DNA in pET-3a, expressed in BL21(DE3)pLysS; no affinity tag stated. The start-primer sequence matches current K-12 P45543, but complete experimental sequence identity is not established. Approximately 28-kDa gel-filtration behavior supports the monomer assignment.; Evidence PMID:12147680: Methods describe the BL21(DE3) donor and pET-3a expression in BL21(DE3)pLysS; Results assigns monomeric behavior from gel filtration. (p42524 Overexpression and purification of fructoselysine 6-kinase; p42526 Expression and characterization; Fig5 caption) experimental construct Fructoselysine 6-kinase [experimental construct: FrlD from BL21(DE3) genomic DNA in pET-3a, expressed in BL21(DE3)pLysS; no affinity tag stated. The…] fructoselysine 6-phosphate deglycase; MetaCyc:CPLX0-821; Declared type: complex; experimental construct: Active first-AUG FrlB from BL21(DE3) DNA, pET-3a expression in BL21(DE3)pLysS. Its start primer matches current P0AC00 residues 1 onward; complete donor sequence identity is unproved. The second-AUG construct was insoluble/inactive. Approximately 450-kDa gel filtration suggests a dodecamer; the assembly trace was not shown.; Evidence PMID:12147680: The first-AUG construct is active, whereas the second-AUG product is insoluble with no detected activity. An approximately 450-kDa apparent mass suggests a dodecamer. (p42524 Overexpression and purification of fructoselysine-6-phosphate deglycase; p42527 Expression and characterization of putative deglycase) experimental construct fructoselysine 6-phosphate deglycase [experimental construct: Active first-AUG FrlB from BL21(DE3) DNA, pET-3a expression in BL21(DE3)pLysS. Its start primer…] fructoselysine 6-phosphate deglycase; MetaCyc:CPLX0-821; Declared type: complex; experimental construct: Active first-AUG FrlB from BL21(DE3) DNA, pET-3a expression in BL21(DE3)pLysS. Its start primer matches current P0AC00 residues 1 onward; complete donor sequence identity is unproved. The second-AUG construct was insoluble/inactive. Approximately 450-kDa gel filtration suggests a dodecamer; the assembly trace was not shown.; Evidence PMID:12147680: The first-AUG construct is active, whereas the second-AUG product is insoluble with no detected activity. An approximately 450-kDa apparent mass suggests a dodecamer. (p42524 Overexpression and purification of fructoselysine-6-phosphate deglycase; p42527 Expression and characterization of putative deglycase) experimental construct fructoselysine 6-phosphate deglycase [experimental construct: Active first-AUG FrlB from BL21(DE3) DNA, pET-3a expression in BL21(DE3)pLysS. Its start primer…] Probable fructoselysine utilization operon transcriptional repressor; UniProtKB:P45544; Declared type: protein; experimental construct: E. coli FrlR expressed from pBAD33 in BW25113/JW5698 PfrlABCD-lux reporter comparisons. Exact construction/primer tables were unavailable; full insert identity to current K-12 P45544 is not independently established. The measured target is a shared-promoter reporter, not each endogenous transcript or an MG1655 gene instance.; Evidence PMID:32979851: BW25113 and frlR-deleted JW5698 lux reporters are compared with ectopic pBAD33-FrlR; construction details are referred to supporting Table S2. (Thesis PDF pp89–90 (article pp5–6), Figs5b/6; Methods4.5 article p13 / PDF97) experimental construct Probable fructoselysine utilization operon transcriptional repressor [experimental construct: E. coli FrlR expressed from pBAD33 in BW25113/JW5698 PfrlABCD-lux reporter comparisons. Exact…] Probable fructoselysine utilization operon transcriptional repressor; UniProtKB:P45544; Declared type: protein; experimental construct: Separate in vitro preparation: His6-SUMO-FrlR expressed in BL21(DE3), cleaved with His6-Ulp1 and recovered after metal-affinity cleanup. Exact supplementary construct sequence is unverified. Operator DNA is the assay target; the reference protein ID does not establish a particular MG1655 promoter or full-length native dimer.; Evidence PMID:32979851: Protein purification uses His6-SUMO expression in BL21(DE3), His6-Ulp1 cleavage and recovery of tag-free FrlR; SPR compares operator-bearing and control DNA. (Thesis PDF pp97–98 (article pp13–14), Methods4.6–4.7) experimental construct Probable fructoselysine utilization operon transcriptional repressor [experimental construct: Separate in vitro preparation: His6-SUMO-FrlR expressed in BL21(DE3), cleaved with His6-Ulp1 and…] RNA polymerase sigma factor RpoH; UniProtKB:P0AGB3; Declared type: protein; experimental construct: pBAD33-rpoH overexpression in BW25113 shared-promoter lux reporter assays, with promoter truncations and temperature conditions specified by Figure 5c and Methods4.5. Exact supplementary insert sequence was not inspected; current K-12 RpoH denotes gene-product identity.; Evidence PMID:32979851: The reporter experiment overproduces RpoH from pBAD33; Methods specifies adjusted measurement temperatures for rpoH analysis. (Thesis PDF pp89–90 Fig5c and Results2.3; PDF97 Methods4.5) experimental construct RNA polymerase sigma factor RpoH [experimental construct: pBAD33-rpoH overexpression in BW25113 shared-promoter lux reporter assays, with promoter…] DNA-binding transcriptional dual regulator CRP; UniProtKB:P0ACJ8; Declared type: protein; experimental construct: Indirect CRP attribution in BW25113/JW3778 cyaA and PfrlABCD-lux reporter comparisons. CRP itself was neither deleted nor purified in these experiments; promoter DNA and reporter context were engineered. Exact equivalence of every tested sequence to the reference K-12 molecules is not established.; Evidence PMID:32979851: The cAMP reporter comparisons use cyaA-deleted JW3778, ectopic cyaA and truncated promoter fusions; the CRP-directed interpretation is indirect. (Thesis PDF pp89–90 Fig5d and Results2.3; Methods4.5 PDF97) experimental construct DNA-binding transcriptional dual regulator CRP [experimental construct: Indirect CRP attribution in BW25113/JW3778 cyaA and PfrlABCD-lux reporter comparisons. CRP itself…]

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Escherichia coli K-12
NCBITaxon:83333		taxon
Escherichia coli str. K-12 substr. MG1655
NCBITaxon:511145		taxon
Escherichia coli BW25113
NCBITaxon:679895		taxon
psicosyllysine(1+)
CHEBI:61403		small molecule
fructosyllysine(1+)
CHEBI:61393		small molecule
ATP(4-)
CHEBI:30616		small molecule
fructoselysine 6-phosphate(1-)
CHEBI:61392		small molecule
ADP(3-)
CHEBI:456216		small molecule
hydron
CHEBI:15378		small molecule
water
CHEBI:15377		small molecule
beta-D-glucose 6-phosphate(2-)
CHEBI:58247		small molecule
L-lysinium(1+)
CHEBI:32551		small molecule
nickel(2+)
CHEBI:49786		cofactor
cobalt(2+)
CHEBI:48828		cofactor
iron(2+)
CHEBI:29033		cofactor
manganese(2+)
CHEBI:29035		cofactor
zinc(2+)
CHEBI:29105		small molecule
Fructoselysine 3-epimerase
UniProtKB:P45541		protein
Fructoselysine 6-kinase
UniProtKB:P45543		protein
Fructoselysine 6-phosphate deglycase
UniProtKB:P0AC00		protein
Probable fructoselysine/psicoselysine transporter FrlA
UniProtKB:P45539		protein
Probable fructoselysine utilization operon transcriptional repressor
UniProtKB:P45544		protein
RNA polymerase sigma factor RpoH
UniProtKB:P0AGB3		protein
DNA-binding transcriptional dual regulator CRP
UniProtKB:P0ACJ8		protein
fructoselysine 3-epimerase
MetaCyc:CPLX0-3861		complex
fructoselysine 6-phosphate deglycase
MetaCyc:CPLX0-821		complex
N(6)-(D-psicosyl)-L-lysine => N(6)-(D-fructosyl)-L-lysine
RHEA:28391		molecular activityleft to right
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		molecular activityleft to right
fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine
MetaCyc:RXN0-963		molecular activityleft to right

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
MetaCyc:PWY0-521has_part

Native core membership; exact chemistry and the selected directional Rhea member are independently checked. Native RXN0-4841 is reversible with opposite printed orientation; its R2L pathway layout equals this Rhea L2R psicoselysine entry.

N(6)-(D-psicosyl)-L-lysine => N(6)-(D-fructosyl)-L-lysine
RHEA:28391
  • MetaCyc:PWY0-521

    Source assertion: The native reaction-list contains RXN0-4841.

    Source location/ptools-xml/Pathway/reaction-list/Reaction[@frameid="RXN0-4841"]
  • MetaCyc:RXN0-4841

    Source assertion: The native reaction directly cross-references Rhea 28393; its complete left and right sides and native direction are retained in the same original.

    Source location/ptools-xml/Reaction/dblink[dblink-db="RHEA"]; left; right; reaction-direction
  • RHEA:28391

    Source assertion: RHEA:28390 has directional member RHEA:28391; its explicit substrate/product assignment selects catabolic traversal.

    Source locationAggregate rdf:Description[@rdf:about="http://rdf.rhea-db.org/28390"]/rh:directionalReaction; rdf:Description[@rdf:about="http://rdf.rhea-db.org/28391"]/rh:substrates and rh:products
psicosyllysine(1+)
CHEBI:61403		consumes

Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.

N(6)-(D-psicosyl)-L-lysine => N(6)-(D-fructosyl)-L-lysine
RHEA:28391
  • RHEA:28391

    Source assertion: RHEA:28391 places CHEBI:61403 on its substrates side with coefficient 1 in rh:contains1.

    Source locationAggregate rdf:Description by rdf:about: http://rdf.rhea-db.org/28391/rh:substrates -> http://rdf.rhea-db.org/28390_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_28390_compound_6449/rh:compound -> http://rdf.rhea-db.org/Compound_6449/rh:chebi
N(6)-(D-psicosyl)-L-lysine => N(6)-(D-fructosyl)-L-lysine
RHEA:28391		produces

Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.

fructosyllysine(1+)
CHEBI:61393
  • RHEA:28391

    Source assertion: RHEA:28391 places CHEBI:61393 on its products side with coefficient 1 in rh:contains1.

    Source locationAggregate rdf:Description by rdf:about: http://rdf.rhea-db.org/28391/rh:products -> http://rdf.rhea-db.org/28390_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_28390_compound_6448/rh:compound -> http://rdf.rhea-db.org/Compound_6448/rh:chebi
MetaCyc:PWY0-521has_part

Native core membership; exact chemistry and the selected directional Rhea member are independently checked.

N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379
  • MetaCyc:PWY0-521

    Source assertion: The native reaction-list contains RXN0-962.

    Source location/ptools-xml/Pathway/reaction-list/Reaction[@frameid="RXN0-962"]
  • MetaCyc:RXN0-962

    Source assertion: The native reaction directly cross-references Rhea 28379; its complete left and right sides and native direction are retained in the same original.

    Source location/ptools-xml/Reaction/dblink[dblink-db="RHEA"]; left; right; reaction-direction
  • RHEA:28379

    Source assertion: RHEA:28378 has directional member RHEA:28379; its explicit substrate/product assignment selects catabolic traversal.

    Source locationAggregate rdf:Description[@rdf:about="http://rdf.rhea-db.org/28378"]/rh:directionalReaction; rdf:Description[@rdf:about="http://rdf.rhea-db.org/28379"]/rh:substrates and rh:products
fructosyllysine(1+)
CHEBI:61393		consumes

Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.

N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379
  • RHEA:28379

    Source assertion: RHEA:28379 places CHEBI:61393 on its substrates side with coefficient 1 in rh:contains1.

    Source locationAggregate rdf:Description by rdf:about: http://rdf.rhea-db.org/28379/rh:substrates -> http://rdf.rhea-db.org/28378_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_28378_compound_6448/rh:compound -> http://rdf.rhea-db.org/Compound_6448/rh:chebi
ATP(4-)
CHEBI:30616		consumes

Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.

N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379
  • RHEA:28379

    Source assertion: RHEA:28379 places CHEBI:30616 on its substrates side with coefficient 1 in rh:contains1.

    Source locationAggregate rdf:Description by rdf:about: http://rdf.rhea-db.org/28379/rh:substrates -> http://rdf.rhea-db.org/28378_L/rh:contains1 -> http://rdf.rhea-db.org/Participant_28378_compound_6372/rh:compound -> http://rdf.rhea-db.org/Compound_6372/rh:chebi
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		produces

Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.

fructoselysine 6-phosphate(1-)
CHEBI:61392
  • RHEA:28379

    Source assertion: RHEA:28379 places CHEBI:61392 on its products side with coefficient 1 in rh:contains1.

    Source locationAggregate rdf:Description by rdf:about: http://rdf.rhea-db.org/28379/rh:products -> http://rdf.rhea-db.org/28378_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_28378_compound_6447/rh:compound -> http://rdf.rhea-db.org/Compound_6447/rh:chebi
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		produces

Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.

ADP(3-)
CHEBI:456216
  • RHEA:28379

    Source assertion: RHEA:28379 places CHEBI:456216 on its products side with coefficient 1 in rh:contains1.

    Source locationAggregate rdf:Description by rdf:about: http://rdf.rhea-db.org/28379/rh:products -> http://rdf.rhea-db.org/28378_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_28378_compound_2710/rh:compound -> http://rdf.rhea-db.org/Compound_2710/rh:chebi
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		produces

Complete formal reaction side, coefficient one; auxiliary substrates and products are retained.

hydron
CHEBI:15378
  • RHEA:28379

    Source assertion: RHEA:28379 places CHEBI:15378 on its products side with coefficient 1 in rh:contains1.

    Source locationAggregate rdf:Description by rdf:about: http://rdf.rhea-db.org/28379/rh:products -> http://rdf.rhea-db.org/28378_R/rh:contains1 -> http://rdf.rhea-db.org/Participant_28378_compound_3249/rh:compound -> http://rdf.rhea-db.org/Compound_3249/rh:chebi
MetaCyc:PWY0-521has_part

Native core membership preserves the beta-D-glucose 6-phosphate product; the source Rhea cross-reference has broader anomer scope and is not treated as exact. This catabolic direction does not assert thermodynamic irreversibility; FrlB also catalyzes the reverse conversion in vitro.

fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine
MetaCyc:RXN0-963
  • MetaCyc:PWY0-521

    Source assertion: The native reaction-list contains RXN0-963.

    Source location/ptools-xml/Pathway/reaction-list/Reaction[@frameid="RXN0-963"]
  • MetaCyc:RXN0-963

    Source assertion: The complete native reaction converts FRUCTOSELYSINE-6-PHOSPHATE and WATER to GLC-6-P and LYS, with LEFT-TO-RIGHT direction.

    Source location/ptools-xml/Reaction/left; right; reaction-direction
fructoselysine 6-phosphate(1-)
CHEBI:61392		consumes

Complete native deglycase side; exact beta-D-glucose 6-phosphate chemistry is retained. This stereochemical scope is a native database assertion, not automatically an independently measured primary-study product.

fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine
MetaCyc:RXN0-963
  • MetaCyc:RXN0-963

    Source assertion: The complete native left side contains FRUCTOSELYSINE-6-PHOSPHATE.

    Source location/ptools-xml/Reaction/left/Compound[@frameid="FRUCTOSELYSINE-6-PHOSPHATE"]
  • MetaCyc:FRUCTOSELYSINE-6-PHOSPHATE

    Source assertion: The native compound has a direct CHEBI:61392 cross-reference and matching complete InChI.

    Source location/ptools-xml/Compound/dblink[dblink-db="CHEBI"]; inchi
water
CHEBI:15377		consumes

Complete native deglycase side; exact beta-D-glucose 6-phosphate chemistry is retained. This stereochemical scope is a native database assertion, not automatically an independently measured primary-study product.

fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine
MetaCyc:RXN0-963
  • MetaCyc:RXN0-963

    Source assertion: The complete native left side contains WATER.

    Source location/ptools-xml/Reaction/left/Compound[@frameid="WATER"]
  • MetaCyc:WATER

    Source assertion: The native compound has a direct CHEBI:15377 cross-reference and matching complete InChI.

    Source location/ptools-xml/Compound/dblink[dblink-db="CHEBI"]; inchi
fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine
MetaCyc:RXN0-963		produces

Complete native deglycase side; exact beta-D-glucose 6-phosphate chemistry is retained. This stereochemical scope is a native database assertion, not automatically an independently measured primary-study product.

beta-D-glucose 6-phosphate(2-)
CHEBI:58247
  • MetaCyc:RXN0-963

    Source assertion: The complete native right side contains GLC-6-P.

    Source location/ptools-xml/Reaction/right/Compound[@frameid="GLC-6-P"]
  • MetaCyc:GLC-6-P

    Source assertion: The native compound has a direct CHEBI:58247 cross-reference and matching complete InChI.

    Source location/ptools-xml/Compound/dblink[dblink-db="CHEBI"]; inchi
fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine
MetaCyc:RXN0-963		produces

Complete native deglycase side; exact beta-D-glucose 6-phosphate chemistry is retained. This stereochemical scope is a native database assertion, not automatically an independently measured primary-study product.

L-lysinium(1+)
CHEBI:32551
  • MetaCyc:RXN0-963

    Source assertion: The complete native right side contains LYS.

    Source location/ptools-xml/Reaction/right/Compound[@frameid="LYS"]
  • MetaCyc:LYS

    Source assertion: The native compound has a direct CHEBI:32551 cross-reference and matching complete InChI.

    Source location/ptools-xml/Compound/dblink[dblink-db="CHEBI"]; inchi
N(6)-(D-psicosyl)-L-lysine => N(6)-(D-fructosyl)-L-lysine
RHEA:28391		provides_input_for

The selected upstream event provides fructoselysine to the next reaction; fructoselysine may also enter directly at the kinase.

N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379
  • MetaCyc:PWY0-521

    Source assertion: RXN0-962 names RXN0-4841 as its predecessor; adjacent layouts share fructoselysine.

    Source location/ptools-xml/Pathway/reaction-ordering[Reaction/@frameid="RXN0-962"]; reaction-layout
N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379		provides_input_for

The selected upstream event provides fructoselysine 6-phosphate to the next reaction; fructoselysine may also enter directly at the kinase.

fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine
MetaCyc:RXN0-963
  • MetaCyc:PWY0-521

    Source assertion: RXN0-963 names RXN0-962 as its predecessor; adjacent layouts share fructoselysine 6-phosphate.

    Source location/ptools-xml/Pathway/reaction-ordering[Reaction/@frameid="RXN0-963"]; reaction-layout
fructoselysine 3-epimerase
MetaCyc:CPLX0-3861		has_part

Native homomer annotation contains 8 copies of the linked polypeptide. Source assembly inference does not establish a solved structure or exact experimental construct.

Fructoselysine 3-epimerase
UniProtKB:P45541
  • MetaCyc:CPLX0-3861

    Source assertion: The native component list specifies 8 copies of G7724-MONOMER.

    Source location/ptools-xml/Protein/component[Protein/@frameid="G7724-MONOMER"]/coefficient
  • MetaCyc:G7724-MONOMER

    Source assertion: The native polypeptide directly cross-references UniProt P45541.

    Source location/ptools-xml/Protein/dblink[dblink-db="UNIPROT"]
fructoselysine 6-phosphate deglycase
MetaCyc:CPLX0-821		has_part

Native homomer annotation contains 12 copies of the linked polypeptide. Source assembly inference does not establish a solved structure or exact experimental construct.

Fructoselysine 6-phosphate deglycase
UniProtKB:P0AC00
  • MetaCyc:CPLX0-821

    Source assertion: The native component list specifies 12 copies of G7723-MONOMER.

    Source location/ptools-xml/Protein/component[Protein/@frameid="G7723-MONOMER"]/coefficient
  • MetaCyc:G7723-MONOMER

    Source assertion: The native polypeptide directly cross-references UniProt P0AC00.

    Source location/ptools-xml/Protein/dblink[dblink-db="UNIPROT"]
fructoselysine 3-epimerase
MetaCyc:CPLX0-3861		catalyzes

Curated native catalytic assignment; this annotation is separate from experimental preparation claims.

N(6)-(D-psicosyl)-L-lysine => N(6)-(D-fructosyl)-L-lysine
RHEA:28391
  • MetaCyc:CPLX0-3861

    Source assertion: The native catalyst activity has an enzyme backlink and reaction RXN0-4841.

    Source location/ptools-xml/Protein/catalyzes/Enzymatic-Reaction[reaction/Reaction/@frameid="RXN0-4841"]/enzyme; reaction
  • MetaCyc:RXN0-4841

    Source assertion: The native reaction directly cross-references Rhea 28393; its complete left and right sides and native direction are retained in the same original.

    Source location/ptools-xml/Reaction/dblink[dblink-db="RHEA"]; left; right; reaction-direction
  • RHEA:28391

    Source assertion: RHEA:28390 has directional member RHEA:28391; its explicit substrate/product assignment selects catabolic traversal.

    Source locationAggregate rdf:Description[@rdf:about="http://rdf.rhea-db.org/28390"]/rh:directionalReaction; rdf:Description[@rdf:about="http://rdf.rhea-db.org/28391"]/rh:substrates and rh:products
Fructoselysine 6-kinase
UniProtKB:P45543		catalyzes

Curated native catalytic assignment; this annotation is separate from experimental preparation claims.

N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379
  • MetaCyc:G7726-MONOMER

    Source assertion: The native catalyst activity has an enzyme backlink and reaction RXN0-962.

    Source location/ptools-xml/Protein/catalyzes/Enzymatic-Reaction[reaction/Reaction/@frameid="RXN0-962"]/enzyme; reaction
  • MetaCyc:G7726-MONOMER

    Source assertion: The native protein links directly to UniProt P45543.

    Source location/ptools-xml/Protein/dblink[dblink-db="UNIPROT"]
  • MetaCyc:RXN0-962

    Source assertion: The native reaction directly cross-references Rhea 28379; its complete left and right sides and native direction are retained in the same original.

    Source location/ptools-xml/Reaction/dblink[dblink-db="RHEA"]; left; right; reaction-direction
  • RHEA:28379

    Source assertion: RHEA:28378 has directional member RHEA:28379; its explicit substrate/product assignment selects catabolic traversal.

    Source locationAggregate rdf:Description[@rdf:about="http://rdf.rhea-db.org/28378"]/rh:directionalReaction; rdf:Description[@rdf:about="http://rdf.rhea-db.org/28379"]/rh:substrates and rh:products
fructoselysine 6-phosphate deglycase
MetaCyc:CPLX0-821		catalyzes

Curated native catalytic assignment; this annotation is separate from experimental preparation claims.

fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine
MetaCyc:RXN0-963
  • MetaCyc:CPLX0-821

    Source assertion: The native catalyst activity has an enzyme backlink and reaction RXN0-963.

    Source location/ptools-xml/Protein/catalyzes/Enzymatic-Reaction[reaction/Reaction/@frameid="RXN0-963"]/enzyme; reaction
  • MetaCyc:RXN0-963

    Source assertion: The complete native reaction converts FRUCTOSELYSINE-6-PHOSPHATE and WATER to GLC-6-P and LYS, with LEFT-TO-RIGHT direction.

    Source location/ptools-xml/Reaction/left; right; reaction-direction
fructoselysine 3-epimerase
MetaCyc:CPLX0-3861

experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.

Qualification evidence
  • PMID:14641112

    Source assertion: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer.

    Source locationp1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization
  • UniProtKB:P45541

    Source assertion: The current reference sequence contains 276 residues, with terminal arginine.

    Source locationprimaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value
catalyzes

Purified recombinant FrlC interconverts the two substrates in vitro; Figure 4 includes both starting substrates and enzyme-free controls. The selected graph orientation follows psicoselysine catabolism.

N(6)-(D-psicosyl)-L-lysine => N(6)-(D-fructosyl)-L-lysine
RHEA:28391
  • PMID:14641112

    Source assertion: Purified FrlC reversibly interconverts psicoselysine and fructoselysine; controls without enzyme remain stable.

    Source locationpp1049–1051 Kinetic properties and catalysed reaction; Fig4 and its caption
fructoselysine 3-epimerase
MetaCyc:CPLX0-3861

experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.

Qualification evidence
  • PMID:14641112

    Source assertion: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer.

    Source locationp1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization
  • UniProtKB:P45541

    Source assertion: The current reference sequence contains 276 residues, with terminal arginine.

    Source locationprimaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value
has_cofactor

Alternative in vitro metal support; nickel and cobalt enhance activity more than iron or manganese. The extract assay includes 1 mM MgCl2. Physiological occupancy, fixed stoichiometry and simultaneous binding of these alternatives are not established.

nickel(2+)
CHEBI:49786
  • PMID:14641112

    Source assertion: Ni(2+) enhances the FrlC extract detritiation assay; the relative enhancement is Ni > Co > Fe approximately Mn.

    Source locationp1049 Expression and characterization; Fig2 p1050 and complete caption
  • UniProtKB:P45541

    Source assertion: COFACTOR lists Ni(2+) and Co(2+) and describes weaker in vitro support by Fe(2+) or Mn(2+), with ECO:0000269/PMID:14641112.

    Source locationcomments[commentType="COFACTOR"].cofactors; note.texts
fructoselysine 3-epimerase
MetaCyc:CPLX0-3861

experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.

Qualification evidence
  • PMID:14641112

    Source assertion: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer.

    Source locationp1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization
  • UniProtKB:P45541

    Source assertion: The current reference sequence contains 276 residues, with terminal arginine.

    Source locationprimaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value
has_cofactor

Alternative in vitro metal support; nickel and cobalt enhance activity more than iron or manganese. The extract assay includes 1 mM MgCl2. Physiological occupancy, fixed stoichiometry and simultaneous binding of these alternatives are not established.

cobalt(2+)
CHEBI:48828
  • PMID:14641112

    Source assertion: Co(2+) enhances the FrlC extract detritiation assay; the relative enhancement is Ni > Co > Fe approximately Mn.

    Source locationp1049 Expression and characterization; Fig2 p1050 and complete caption
  • UniProtKB:P45541

    Source assertion: COFACTOR lists Ni(2+) and Co(2+) and describes weaker in vitro support by Fe(2+) or Mn(2+), with ECO:0000269/PMID:14641112.

    Source locationcomments[commentType="COFACTOR"].cofactors; note.texts
fructoselysine 3-epimerase
MetaCyc:CPLX0-3861

experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.

Qualification evidence
  • PMID:14641112

    Source assertion: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer.

    Source locationp1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization
  • UniProtKB:P45541

    Source assertion: The current reference sequence contains 276 residues, with terminal arginine.

    Source locationprimaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value
has_cofactor

Alternative in vitro metal support; nickel and cobalt enhance activity more than iron or manganese. The extract assay includes 1 mM MgCl2. Physiological occupancy, fixed stoichiometry and simultaneous binding of these alternatives are not established.

iron(2+)
CHEBI:29033
  • PMID:14641112

    Source assertion: Fe(2+) enhances the FrlC extract detritiation assay; the relative enhancement is Ni > Co > Fe approximately Mn.

    Source locationp1049 Expression and characterization; Fig2 p1050 and complete caption
  • UniProtKB:P45541

    Source assertion: COFACTOR lists Ni(2+) and Co(2+) and describes weaker in vitro support by Fe(2+) or Mn(2+), with ECO:0000269/PMID:14641112.

    Source locationcomments[commentType="COFACTOR"].cofactors; note.texts
fructoselysine 3-epimerase
MetaCyc:CPLX0-3861

experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.

Qualification evidence
  • PMID:14641112

    Source assertion: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer.

    Source locationp1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization
  • UniProtKB:P45541

    Source assertion: The current reference sequence contains 276 residues, with terminal arginine.

    Source locationprimaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value
has_cofactor

Alternative in vitro metal support; nickel and cobalt enhance activity more than iron or manganese. The extract assay includes 1 mM MgCl2. Physiological occupancy, fixed stoichiometry and simultaneous binding of these alternatives are not established.

manganese(2+)
CHEBI:29035
  • PMID:14641112

    Source assertion: Mn(2+) enhances the FrlC extract detritiation assay; the relative enhancement is Ni > Co > Fe approximately Mn.

    Source locationp1049 Expression and characterization; Fig2 p1050 and complete caption
  • UniProtKB:P45541

    Source assertion: COFACTOR lists Ni(2+) and Co(2+) and describes weaker in vitro support by Fe(2+) or Mn(2+), with ECO:0000269/PMID:14641112.

    Source locationcomments[commentType="COFACTOR"].cofactors; note.texts
zinc(2+)
CHEBI:29105		inhibits

In vitro inhibition in the recombinant extract assay: 10 micromolar Zn2+ completely inhibits reported activity, with underlying results not shown. No physiological regulatory role is inferred.

fructoselysine 3-epimerase
MetaCyc:CPLX0-3861

experimental construct: Recombinant FrlC: Methods use BL21(DE3) DNA, pET-3a and BL21(DE3)pLysS; Results calls the region K12. Figure 1 reports 275 residues versus current P45541 length 276 (extra terminal Arg). Exact full-length MG1655 equivalence is not asserted. Octamer assignment derives from approximately 260-kDa gel filtration, results not shown.

Qualification evidence
  • PMID:14641112

    Source assertion: Methods specify BL21(DE3) DNA and pET-3a expression; Results describes K12 resequencing and a 275-residue protein. Gel filtration suggests an octamer.

    Source locationp1048 Overexpression and purification; Results Establishment of sequence; p1049 Fig1 and Expression and characterization
  • UniProtKB:P45541

    Source assertion: The current reference sequence contains 276 residues, with terminal arginine.

    Source locationprimaryAccession; entryAudit.sequenceVersion; sequence.length; sequence.value
  • PMID:14641112

    Source assertion: Zn2+ completely inhibited activity at 10 micromolar; these results are reported as not shown.

    Source locationp1049 Expression and characterization, first paragraph
Probable fructoselysine/psicoselysine transporter FrlA
UniProtKB:P45539

experimental construct: Indirect gene-product attribution from the FB21138 frlA Tn5 growth comparison at 20 mM substrates. The Materials passage does not specify this mutant background; the separately described BL21 cultures do not establish it. No complementation or purified FrlA transport assay is reported.

Qualification evidence
  • PMID:14641112

    Source assertion: FB21138 is a Blattner-collection frlA Tn5 mutant; growth was followed in M9 with 20 mM substrates. The reported phenotype lacks a rescue experiment.

    Source locationp1047 Materials; p1048 Growth of E. coli on psicoselysine; p1051 growth Results
enables

FrlA participation is inferred from the growth phenotype and reviewed probable-transporter annotation. Tn5 polarity and lack of rescue limit gene-product attribution; transport itself, coupling and stoichiometry were not measured. FrlA is context, not a core reaction member.

MetaCyc:PWY0-521
  • PMID:14641112

    Source assertion: The frlA Tn5 mutant FB21138 does not grow on fructoselysine or psicoselysine but does grow on glucose; Figure 5 treats FrlA transport as presumptive.

    Source locationp1047 Materials; p1051 Growth of E. coli on psicoselysine and Fig5 caption
  • UniProtKB:P45539

    Source assertion: FUNCTION describes likely fructoselysine/psicoselysine transport with ECO:0000305; DISRUPTION PHENOTYPE cites PMID:14641112 with ECO:0000269.

    Source locationcomments[commentType="FUNCTION"].texts; comments[commentType="DISRUPTION PHENOTYPE"].texts
Fructoselysine 6-kinase
UniProtKB:P45543

experimental construct: FrlD from BL21(DE3) genomic DNA in pET-3a, expressed in BL21(DE3)pLysS; no affinity tag stated. The start-primer sequence matches current K-12 P45543, but complete experimental sequence identity is not established. Approximately 28-kDa gel-filtration behavior supports the monomer assignment.

Qualification evidence
  • PMID:12147680

    Source assertion: Methods describe the BL21(DE3) donor and pET-3a expression in BL21(DE3)pLysS; Results assigns monomeric behavior from gel filtration.

    Source locationp42524 Overexpression and purification of fructoselysine 6-kinase; p42526 Expression and characterization; Fig5 caption
catalyzes

Recombinant FrlD catalyzes ATP-dependent C6 phosphorylation. Product NMR establishes phosphate position while retaining multiple solution forms at pH 4.1. Mg salts are assay conditions; their inclusion alone does not establish an indispensable cofactor. NADH and phosphoenolpyruvate belong to coupled detection.

N(6)-(D-fructosyl)-L-lysine + ATP => N(6)-(6-phospho-D-fructosyl)-L-lysine + ADP + H(+)
RHEA:28379
  • PMID:12147680

    Source assertion: The purified kinase phosphorylates fructoselysine using ATP; product characterization assigns the phosphate to carbon 6.

    Source locationp42524 enzyme assay Methods; pp42526–42527 product characterization and Fig6 caption
fructoselysine 6-phosphate deglycase
MetaCyc:CPLX0-821

experimental construct: Active first-AUG FrlB from BL21(DE3) DNA, pET-3a expression in BL21(DE3)pLysS. Its start primer matches current P0AC00 residues 1 onward; complete donor sequence identity is unproved. The second-AUG construct was insoluble/inactive. Approximately 450-kDa gel filtration suggests a dodecamer; the assembly trace was not shown.

Qualification evidence
  • PMID:12147680

    Source assertion: The first-AUG construct is active, whereas the second-AUG product is insoluble with no detected activity. An approximately 450-kDa apparent mass suggests a dodecamer.

    Source locationp42524 Overexpression and purification of fructoselysine-6-phosphate deglycase; p42527 Expression and characterization of putative deglycase
catalyzes

Recombinant FrlB supports reversible cleavage to lysine and glucose 6-phosphate, including the reverse condensation experiment. The enzymatic glucose-6-phosphate identification does not establish anomer specificity: the beta product attached to this native reaction remains a separate database assertion.

fructoselysine 6-phosphate + water → beta-D-glucose 6-phosphate + L-lysine
MetaCyc:RXN0-963
  • PMID:12147680

    Source assertion: The active deglycase preparation forms glucose 6-phosphate and lysine; reverse formation of fructoselysine 6-phosphate is measured.

    Source locationp42527 Expression and characterization of putative deglycase; p42528 Fig8 caption and Discussion
  • MetaCyc:GLC-6-P

    Source assertion: The native product compound directly identifies CHEBI:58247 and supplies beta-anomer-specific complete InChI.

    Source location/ptools-xml/Compound/common-name; inchi; dblink[dblink-db="CHEBI"]
zinc(2+)
CHEBI:29105		inhibits

In vitro inhibition: 10 micromolar ZnCl2 completely suppresses the recombinant preparation activity. EGTA/EDTA stimulated activity and later assays included 0.1 mM EGTA; the zinc-test buffer is not separately fully restated. This is not physiological zinc regulation.

fructoselysine 6-phosphate deglycase
MetaCyc:CPLX0-821

experimental construct: Active first-AUG FrlB from BL21(DE3) DNA, pET-3a expression in BL21(DE3)pLysS. Its start primer matches current P0AC00 residues 1 onward; complete donor sequence identity is unproved. The second-AUG construct was insoluble/inactive. Approximately 450-kDa gel filtration suggests a dodecamer; the assembly trace was not shown.

Qualification evidence
  • PMID:12147680

    Source assertion: The first-AUG construct is active, whereas the second-AUG product is insoluble with no detected activity. An approximately 450-kDa apparent mass suggests a dodecamer.

    Source locationp42524 Overexpression and purification of fructoselysine-6-phosphate deglycase; p42527 Expression and characterization of putative deglycase
  • PMID:12147680

    Source assertion: ZnCl2 at 10 micromolar completely suppressed deglycase activity; chelators stimulated activity, and 0.1 mM EGTA was included in subsequent assays.

    Source locationp42527 Expression and characterization of putative deglycase
Probable fructoselysine utilization operon transcriptional repressor
UniProtKB:P45544

experimental construct: E. coli FrlR expressed from pBAD33 in BW25113/JW5698 PfrlABCD-lux reporter comparisons. Exact construction/primer tables were unavailable; full insert identity to current K-12 P45544 is not independently established. The measured target is a shared-promoter reporter, not each endogenous transcript or an MG1655 gene instance.

Qualification evidence
  • PMID:32979851

    Source assertion: BW25113 and frlR-deleted JW5698 lux reporters are compared with ectopic pBAD33-FrlR; construction details are referred to supporting Table S2.

    Source locationThesis PDF pp89–90 (article pp5–6), Figs5b/6; Methods4.5 article p13 / PDF97
inhibits

Transcriptional repression of the shared frlABCD promoter controls pathway expression in the BW25113 reporter system. This process-level context edge does not assert direct inhibition of the pathway enzymes, separate endogenous transcript measurements, regulation of frlR itself, or psicoselysine-specific reporter testing.

MetaCyc:PWY0-521
  • PMID:32979851

    Source assertion: Deleting frlR increases PfrlABCD-lux output; ectopic FrlR represses it. Epsilon-fructoselysine relieves reporter repression, whereas alpha-fructoselysine does not.

    Source locationThesis PDF pp89–90 (article pp5–6), Figs5b/6 and Results2.3
Probable fructoselysine utilization operon transcriptional repressor
UniProtKB:P45544

experimental construct: Separate in vitro preparation: His6-SUMO-FrlR expressed in BL21(DE3), cleaved with His6-Ulp1 and recovered after metal-affinity cleanup. Exact supplementary construct sequence is unverified. Operator DNA is the assay target; the reference protein ID does not establish a particular MG1655 promoter or full-length native dimer.

Qualification evidence
  • PMID:32979851

    Source assertion: Protein purification uses His6-SUMO expression in BL21(DE3), His6-Ulp1 cleavage and recovery of tag-free FrlR; SPR compares operator-bearing and control DNA.

    Source locationThesis PDF pp97–98 (article pp13–14), Methods4.6–4.7
regulates

Operator binding supplies the molecular basis for the separately observed transcriptional repression. SPR measured operator-dependent binding, with reported KD 4.9 nM after active-concentration correction; engineered T7/operator reporters support sequence dependence. This is regulatory context, not a new metabolic event.

MetaCyc:PWY0-521
  • PMID:32979851

    Source assertion: Purified FrlR binds operator-bearing DNA in SPR. Operator mutations alter the engineered reporter response, supporting sequence-dependent DNA recognition.

    Source locationThesis PDF pp91–93 (article pp7–9), Results2.4 and Fig7; Methods4.6/4.9
RNA polymerase sigma factor RpoH
UniProtKB:P0AGB3

experimental construct: pBAD33-rpoH overexpression in BW25113 shared-promoter lux reporter assays, with promoter truncations and temperature conditions specified by Figure 5c and Methods4.5. Exact supplementary insert sequence was not inspected; current K-12 RpoH denotes gene-product identity.

Qualification evidence
  • PMID:32979851

    Source assertion: The reporter experiment overproduces RpoH from pBAD33; Methods specifies adjusted measurement temperatures for rpoH analysis.

    Source locationThesis PDF pp89–90 Fig5c and Results2.3; PDF97 Methods4.5
activates

Positive transcriptional context from RpoH overexpression and promoter-truncation reporters. This is not a purified sigma-factor binding experiment, direct enzyme activation, individual endogenous-transcript measurement, or a host-adaptation experiment.

MetaCyc:PWY0-521
  • PMID:32979851

    Source assertion: Arabinose-induced rpoH on pBAD33 increases PfrlABCD-lux output in promoter-truncation experiments.

    Source locationThesis PDF pp89–90 (article pp5–6), Fig5c; Methods4.5 article p13 / PDF97
DNA-binding transcriptional dual regulator CRP
UniProtKB:P0ACJ8

experimental construct: Indirect CRP attribution in BW25113/JW3778 cyaA and PfrlABCD-lux reporter comparisons. CRP itself was neither deleted nor purified in these experiments; promoter DNA and reporter context were engineered. Exact equivalence of every tested sequence to the reference K-12 molecules is not established.

Qualification evidence
  • PMID:32979851

    Source assertion: The cAMP reporter comparisons use cyaA-deleted JW3778, ectopic cyaA and truncated promoter fusions; the CRP-directed interpretation is indirect.

    Source locationThesis PDF pp89–90 Fig5d and Results2.3; Methods4.5 PDF97
activates

Inferred CRP/cAMP-dependent positive transcriptional control from cyaA manipulation, glucose response and candidate-CRP-region truncation. The study supplies no new purified CRP binding or crp-deletion experiment. The source differs between 10 and 20 mM glucose descriptions; no single dose is selected here.

MetaCyc:PWY0-521
  • PMID:32979851

    Source assertion: cyaA deletion reduces the reporter and ectopic cyaA restores activity; glucose and promoter truncation alter the response, supporting a CRP/cAMP regulatory interpretation.

    Source locationThesis PDF pp89–90 (article pp5–6), Fig5d and Results2.3; PDF97 Methods4.5

References

Curation history