Mycobacterium tuberculosis trehalose biosynthesis

Reactome:R-MTU-868688

Mycobacterium tuberculosis produces trehalose through OtsA/OtsB and TreY/TreZ routes. TreS is reversible in vitro, but experimental physiological flux consumes trehalose to produce alpha-maltose. Cytosolic catalytic assemblies include OtsA tetramer and magnesium-bound OtsB.

Pathway type

biopax pathway

Organism scope

Mycobacterium tuberculosis NCBITaxon:1773

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Pathway network

26 nodes · 50 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Mycobacterium tuberculosis `NCBITaxon:1773`	taxon
UDP-Glc `CHEBI:58885`	small molecule
G6P `CHEBI:58225`	small molecule
αα-T6P `CHEBI:18283`	small molecule
UDP `CHEBI:58223`	small molecule
H+ `CHEBI:15378`	small molecule
H2O `CHEBI:15377`	small molecule
alpha,alpha-trehalose `CHEBI:16551`	small molecule
Pi `CHEBI:43474`	small molecule
Mg2+ `CHEBI:18420`	cofactor
(1->4)-alpha-D-glucan `CHEBI:15444`	small molecule
glucanotrehalose `CHEBI:134401`	small molecule
OtsA `UniProtKB:P9WN11`	protein
OtsB `UniProtKB:P9WFZ5`	protein
TreS `UniProtKB:P9WQ19`	protein
TreY `UniProtKB:P9WQ21`	protein
TreZ `UniProtKB:P9WQ23`	protein
OtsA tetramer `Reactome:R-MTU-868690`	complex
OtsB-Mg2+ `Reactome:R-MTU-868623`	complex
cytosol `GO:0005829`	cellular component
alpha-maltose `CHEBI:18167`	small molecule
manganese(2+) `CHEBI:29035`	cofactor
Glucose is transferred from UDP-glucose onto glucose-6-phosphate `Reactome:R-MTU-868622`	molecular activity	left to right
Trehalose-6-phosphate is hydrolyzed to trehalose `Reactome:R-MTU-868608`	molecular activity	left to right
TreS interconverts trehalose and alpha-maltose (physiological trehalose consumption) `Reactome:R-MTU-868709`	molecular activity	reversible
1,4-alpha-glucan is converted to glucanotrehalose `Reactome:R-MTU-868658`	molecular activity	left to right
Glucanotrehalose is hydrolyzed to 1,4-alpha-glucan and trehalose `Reactome:R-MTU-868657`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
UDP-Glc `CHEBI:58885`	consumes	Glucose is transferred from UDP-glucose onto glucose-6-phosphate `Reactome:R-MTU-868622`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction1 lists UDP-Glc on its left side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction1/bp:left/#SmallMolecule1`
G6P `CHEBI:58225`	consumes	Glucose is transferred from UDP-glucose onto glucose-6-phosphate `Reactome:R-MTU-868622`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction1 lists G6P on its left side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction1/bp:left/#SmallMolecule2`
Glucose is transferred from UDP-glucose onto glucose-6-phosphate `Reactome:R-MTU-868622`	produces	αα-T6P `CHEBI:18283`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction1 lists αα-T6P on its right side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction1/bp:right/#SmallMolecule3`
Glucose is transferred from UDP-glucose onto glucose-6-phosphate `Reactome:R-MTU-868622`	produces	UDP `CHEBI:58223`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction1 lists UDP on its right side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction1/bp:right/#SmallMolecule4`
Glucose is transferred from UDP-glucose onto glucose-6-phosphate `Reactome:R-MTU-868622`	produces	H+ `CHEBI:15378`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction1 lists H+ on its right side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction1/bp:right/#SmallMolecule5`
Glucose is transferred from UDP-glucose onto glucose-6-phosphate `Reactome:R-MTU-868622`	occurs_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: The physical participants of BioPAX BiochemicalReaction1 are assigned to the cytosol. Source location `#BiochemicalReaction1; participating physicalEntity/bp:cellularLocation/#CellularLocationVocabulary1`
αα-T6P `CHEBI:18283`	consumes	Trehalose-6-phosphate is hydrolyzed to trehalose `Reactome:R-MTU-868608`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction2 lists αα-T6P on its left side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction2/bp:left/#SmallMolecule3`
H2O `CHEBI:15377`	consumes	Trehalose-6-phosphate is hydrolyzed to trehalose `Reactome:R-MTU-868608`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction2 lists H2O on its left side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction2/bp:left/#SmallMolecule6`
Trehalose-6-phosphate is hydrolyzed to trehalose `Reactome:R-MTU-868608`	produces	alpha,alpha-trehalose `CHEBI:16551`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction2 lists alpha,alpha-trehalose on its right side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction2/bp:right/#SmallMolecule7`
Trehalose-6-phosphate is hydrolyzed to trehalose `Reactome:R-MTU-868608`	produces	Pi `CHEBI:43474`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction2 lists Pi on its right side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction2/bp:right/#SmallMolecule8`
Trehalose-6-phosphate is hydrolyzed to trehalose `Reactome:R-MTU-868608`	occurs_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: The physical participants of BioPAX BiochemicalReaction2 are assigned to the cytosol. Source location `#BiochemicalReaction2; participating physicalEntity/bp:cellularLocation/#CellularLocationVocabulary1`
TreS interconverts trehalose and alpha-maltose (physiological trehalose consumption) `Reactome:R-MTU-868709`	occurs_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: The physical participants of BioPAX BiochemicalReaction3 are assigned to the cytosol. Source location `#BiochemicalReaction3; participating physicalEntity/bp:cellularLocation/#CellularLocationVocabulary1`
(1->4)-alpha-D-glucan `CHEBI:15444`	consumes	1,4-alpha-glucan is converted to glucanotrehalose `Reactome:R-MTU-868658`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction4 lists (1,4-alpha-glycosyl)n on its left side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction4/bp:left/#SmallMolecule11`
1,4-alpha-glucan is converted to glucanotrehalose `Reactome:R-MTU-868658`	produces	glucanotrehalose `CHEBI:134401`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction4 lists glucanotrehalose on its right side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction4/bp:right/#SmallMolecule12`
1,4-alpha-glucan is converted to glucanotrehalose `Reactome:R-MTU-868658`	occurs_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: The physical participants of BioPAX BiochemicalReaction4 are assigned to the cytosol. Source location `#BiochemicalReaction4; participating physicalEntity/bp:cellularLocation/#CellularLocationVocabulary1`
glucanotrehalose `CHEBI:134401`	consumes	Glucanotrehalose is hydrolyzed to 1,4-alpha-glucan and trehalose `Reactome:R-MTU-868657`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction5 lists glucanotrehalose on its left side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction5/bp:left/#SmallMolecule12`
H2O `CHEBI:15377`	consumes	Glucanotrehalose is hydrolyzed to 1,4-alpha-glucan and trehalose `Reactome:R-MTU-868657`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction5 lists H2O on its left side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction5/bp:left/#SmallMolecule6`
Glucanotrehalose is hydrolyzed to 1,4-alpha-glucan and trehalose `Reactome:R-MTU-868657`	produces	(1->4)-alpha-D-glucan `CHEBI:15444`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction5 lists (1->4)-alpha-D-glucan on its right side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction5/bp:right/#SmallMolecule13`
Glucanotrehalose is hydrolyzed to 1,4-alpha-glucan and trehalose `Reactome:R-MTU-868657`	produces	alpha,alpha-trehalose `CHEBI:16551`	Reactome:R-MTU-868688 Source assertion: BioPAX BiochemicalReaction5 lists alpha,alpha-trehalose on its right side with LEFT-TO-RIGHT conversionDirection. Source location `#BiochemicalReaction5/bp:right/#SmallMolecule7`
Glucanotrehalose is hydrolyzed to 1,4-alpha-glucan and trehalose `Reactome:R-MTU-868657`	occurs_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: The physical participants of BioPAX BiochemicalReaction5 are assigned to the cytosol. Source location `#BiochemicalReaction5; participating physicalEntity/bp:cellularLocation/#CellularLocationVocabulary1`
OtsA tetramer `Reactome:R-MTU-868690`	catalyzes	Glucose is transferred from UDP-glucose onto glucose-6-phosphate `Reactome:R-MTU-868622`	Reactome:R-MTU-868688 Source assertion: BioPAX Catalysis1 identifies OtsA tetramer as the controller of BiochemicalReaction1. Source location `#Catalysis1/bp:controller/#Complex1; bp:controlled/#BiochemicalReaction1`
OtsB-Mg2+ `Reactome:R-MTU-868623`	catalyzes	Trehalose-6-phosphate is hydrolyzed to trehalose `Reactome:R-MTU-868608`	Reactome:R-MTU-868688 Source assertion: BioPAX Catalysis2 identifies OtsB-Mg2+ as the controller of BiochemicalReaction2. Source location `#Catalysis2/bp:controller/#Complex2; bp:controlled/#BiochemicalReaction2`
TreS `UniProtKB:P9WQ19`	catalyzes	TreS interconverts trehalose and alpha-maltose (physiological trehalose consumption) `Reactome:R-MTU-868709`	Reactome:R-MTU-868688 Source assertion: BioPAX Catalysis3 identifies TreS as the controller of BiochemicalReaction3. Source location `#Catalysis3/bp:controller/#Protein3; bp:controlled/#BiochemicalReaction3`
TreY `UniProtKB:P9WQ21`	catalyzes	1,4-alpha-glucan is converted to glucanotrehalose `Reactome:R-MTU-868658`	Reactome:R-MTU-868688 Source assertion: BioPAX Catalysis4 identifies TreY as the controller of BiochemicalReaction4. Source location `#Catalysis4/bp:controller/#Protein4; bp:controlled/#BiochemicalReaction4`
TreZ `UniProtKB:P9WQ23`	catalyzes	Glucanotrehalose is hydrolyzed to 1,4-alpha-glucan and trehalose `Reactome:R-MTU-868657`	Reactome:R-MTU-868688 Source assertion: BioPAX Catalysis5 identifies TreZ as the controller of BiochemicalReaction5. Source location `#Catalysis5/bp:controller/#Protein5; bp:controlled/#BiochemicalReaction5`
UDP-Glc `CHEBI:58885`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule1 assigns UDP-Glc to the cytosol. Source location `#SmallMolecule1/bp:cellularLocation/#CellularLocationVocabulary1`
G6P `CHEBI:58225`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule2 assigns G6P to the cytosol. Source location `#SmallMolecule2/bp:cellularLocation/#CellularLocationVocabulary1`
αα-T6P `CHEBI:18283`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule3 assigns αα-T6P to the cytosol. Source location `#SmallMolecule3/bp:cellularLocation/#CellularLocationVocabulary1`
UDP `CHEBI:58223`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule4 assigns UDP to the cytosol. Source location `#SmallMolecule4/bp:cellularLocation/#CellularLocationVocabulary1`
H+ `CHEBI:15378`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule5 assigns H+ to the cytosol. Source location `#SmallMolecule5/bp:cellularLocation/#CellularLocationVocabulary1`
H2O `CHEBI:15377`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule6 assigns H2O to the cytosol. Source location `#SmallMolecule6/bp:cellularLocation/#CellularLocationVocabulary1`
alpha,alpha-trehalose `CHEBI:16551`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule7 assigns alpha,alpha-trehalose to the cytosol. Source location `#SmallMolecule7/bp:cellularLocation/#CellularLocationVocabulary1`
Pi `CHEBI:43474`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule8 assigns Pi to the cytosol. Source location `#SmallMolecule8/bp:cellularLocation/#CellularLocationVocabulary1`
Mg2+ `CHEBI:18420`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule9 assigns Mg2+ to the cytosol. Source location `#SmallMolecule9/bp:cellularLocation/#CellularLocationVocabulary1`
(1->4)-alpha-D-glucan `CHEBI:15444`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule11 assigns (1,4-alpha-glycosyl)n to the cytosol. Source location `#SmallMolecule11/bp:cellularLocation/#CellularLocationVocabulary1` Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule13 assigns (1->4)-alpha-D-glucan to the cytosol. Source location `#SmallMolecule13/bp:cellularLocation/#CellularLocationVocabulary1`
glucanotrehalose `CHEBI:134401`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX SmallMolecule12 assigns glucanotrehalose to the cytosol. Source location `#SmallMolecule12/bp:cellularLocation/#CellularLocationVocabulary1`
OtsA `UniProtKB:P9WN11`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX Protein1 assigns OtsA to the cytosol. Source location `#Protein1/bp:cellularLocation/#CellularLocationVocabulary1`
OtsB `UniProtKB:P9WFZ5`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX Protein2 assigns OtsB to the cytosol. Source location `#Protein2/bp:cellularLocation/#CellularLocationVocabulary1`
TreS `UniProtKB:P9WQ19`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX Protein3 assigns TreS to the cytosol. Source location `#Protein3/bp:cellularLocation/#CellularLocationVocabulary1`
TreY `UniProtKB:P9WQ21`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX Protein4 assigns TreY to the cytosol. Source location `#Protein4/bp:cellularLocation/#CellularLocationVocabulary1`
TreZ `UniProtKB:P9WQ23`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX Protein5 assigns TreZ to the cytosol. Source location `#Protein5/bp:cellularLocation/#CellularLocationVocabulary1`
OtsA tetramer `Reactome:R-MTU-868690`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX Complex1 assigns OtsA tetramer to the cytosol. Source location `#Complex1/bp:cellularLocation/#CellularLocationVocabulary1`
OtsA tetramer `Reactome:R-MTU-868690`	has_part	OtsA `UniProtKB:P9WN11`	Reactome:R-MTU-868688 Source assertion: BioPAX Complex1 includes OtsA as a component of OtsA tetramer. Source location `#Complex1/bp:component/#Protein1`
OtsB-Mg2+ `Reactome:R-MTU-868623`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-868688 Source assertion: BioPAX Complex2 assigns OtsB-Mg2+ to the cytosol. Source location `#Complex2/bp:cellularLocation/#CellularLocationVocabulary1`
OtsB-Mg2+ `Reactome:R-MTU-868623`	has_part	OtsB `UniProtKB:P9WFZ5`	Reactome:R-MTU-868688 Source assertion: BioPAX Complex2 includes OtsB as a component of OtsB-Mg2+. Source location `#Complex2/bp:component/#Protein2`
OtsB-Mg2+ `Reactome:R-MTU-868623`	has_part	Mg2+ `CHEBI:18420`	Reactome:R-MTU-868688 Source assertion: BioPAX Complex2 includes Mg2+ as a component of OtsB-Mg2+. Source location `#Complex2/bp:component/#SmallMolecule9`
alpha,alpha-trehalose `CHEBI:16551`	consumes Physiological direction differs from the older maltose-to-trehalose Reactome diagram.	TreS interconverts trehalose and alpha-maltose (physiological trehalose consumption) `Reactome:R-MTU-868709`	PMID:23601637 Source assertion: Genetic and NMR experiments support mycobacterial TreS flux from trehalose to alpha-maltose; the biochemical reaction is reversible. Source location `Results: TreS reaction products and physiological direction; Figure 3`
TreS interconverts trehalose and alpha-maltose (physiological trehalose consumption) `Reactome:R-MTU-868709`	produces Physiological direction differs from the older maltose-to-trehalose Reactome diagram.	alpha-maltose `CHEBI:18167`	PMID:23601637 Source assertion: Genetic and NMR experiments support mycobacterial TreS flux from trehalose to alpha-maltose; the biochemical reaction is reversible. Source location `Results: TreS reaction products and physiological direction; Figure 3`
OtsB `UniProtKB:P9WFZ5`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:15158675); ECO:0000269 (PubMed:15703182)	manganese(2+) `CHEBI:29035`	UniProtKB:P9WFZ5 Source assertion: UniProtKB:P9WFZ5 annotates CHEBI:29035 as a cofactor of this protein. Source location https://rest.uniprot.org/uniprotkb/stream?query=accession%3AP02918+OR+accession%3AP02919+OR+accession%3AP06721+OR+accession%3AP07862+OR+accession%3AP08373+OR+accession%3AP0A6B4+OR+accession%3AP0A6W3+OR+accession%3AP0A749+OR+accession%3AP0A759+OR+accession%3AP0ABG4+OR+accession%3AP0ACC7+OR+accession%3AP0AD68+OR+accession%3AP0AE16+OR+accession%3AP0AF16+OR+accession%3AP0AF18+OR+accession%3AP11880+OR+accession%3AP13016+OR+accession%3AP14900+OR+accession%3AP17169+OR+accession%3AP17443+OR+accession%3AP17952+OR+accession%3AP22188+OR+accession%3AP22634+OR+accession%3AP29012+OR+accession%3AP31120+OR+accession%3AP37773+OR+accession%3AP46022+OR+accession%3AP60932+OR+accession%3AP75949+OR+accession%3AP75959+OR+accession%3AP76008+OR+accession%3AP76535+OR+accession%3AP76577+OR+accession%3AP77272+OR+accession%3AP77570+OR+accession%3AP95189+OR+accession%3AP9WFZ5+OR+accession%3AP9WJM7+OR+accession%3AP9WJM9+OR+accession%3AP9WJN1+OR+accession%3AP9WKI1+OR+accession%3AP9WMY7+OR+accession%3AP9WN11+OR+accession%3AP9WQ19+OR+accession%3AP9WQ21+OR+accession%3AP9WQ23+OR+accession%3AQ2TL65&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:f2a2d9df603885068af58d89787438192b8c396d89a4cc931ac7bbab93a74c5c] #/results/30/comments/0/cofactors/0
OtsB `UniProtKB:P9WFZ5`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:15158675); ECO:0000269 (PubMed:15703182)	Mg2+ `CHEBI:18420`	UniProtKB:P9WFZ5 Source assertion: UniProtKB:P9WFZ5 annotates CHEBI:18420 as a cofactor of this protein. Source location https://rest.uniprot.org/uniprotkb/stream?query=accession%3AP02918+OR+accession%3AP02919+OR+accession%3AP06721+OR+accession%3AP07862+OR+accession%3AP08373+OR+accession%3AP0A6B4+OR+accession%3AP0A6W3+OR+accession%3AP0A749+OR+accession%3AP0A759+OR+accession%3AP0ABG4+OR+accession%3AP0ACC7+OR+accession%3AP0AD68+OR+accession%3AP0AE16+OR+accession%3AP0AF16+OR+accession%3AP0AF18+OR+accession%3AP11880+OR+accession%3AP13016+OR+accession%3AP14900+OR+accession%3AP17169+OR+accession%3AP17443+OR+accession%3AP17952+OR+accession%3AP22188+OR+accession%3AP22634+OR+accession%3AP29012+OR+accession%3AP31120+OR+accession%3AP37773+OR+accession%3AP46022+OR+accession%3AP60932+OR+accession%3AP75949+OR+accession%3AP75959+OR+accession%3AP76008+OR+accession%3AP76535+OR+accession%3AP76577+OR+accession%3AP77272+OR+accession%3AP77570+OR+accession%3AP95189+OR+accession%3AP9WFZ5+OR+accession%3AP9WJM7+OR+accession%3AP9WJM9+OR+accession%3AP9WJN1+OR+accession%3AP9WKI1+OR+accession%3AP9WMY7+OR+accession%3AP9WN11+OR+accession%3AP9WQ19+OR+accession%3AP9WQ21+OR+accession%3AP9WQ23+OR+accession%3AQ2TL65&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:f2a2d9df603885068af58d89787438192b8c396d89a4cc931ac7bbab93a74c5c] #/results/30/comments/0/cofactors/1

References

Reactome:R-MTU-868622 Reactome reaction R-MTU-868622
Reactome:R-MTU-868608 Reactome reaction R-MTU-868608
Reactome:R-MTU-868709 Reactome reaction R-MTU-868709
Reactome:R-MTU-868658 Reactome reaction R-MTU-868658
Reactome:R-MTU-868657 Reactome reaction R-MTU-868657
PMID:10658666 Three pathways for trehalose biosynthesis in mycobacteria
PMID:12473104 Trehalose-phosphate synthase of Mycobacterium tuberculosis. Cloning, expression and properties of the recombinant enzyme
PMID:15158675 Cloning and expression of the trehalose-phosphate phosphatase of Mycobacterium tuberculosis: comparison to the enzyme from Mycobacterium smegmatis
PMID:19177348 Effect of trehalose on protein structure
PMID:15703182 The OtsAB pathway is essential for trehalose biosynthesis in Mycobacterium tuberculosis
PMID:12626396 New insights on trehalose: a multifunctional molecule
PMID:15901732 The crucial role of trehalose and structurally related oligosaccharides in the biosynthesis and transfer of mycolic acids in Corynebacterineae
Reactome:R-MTU-868688 R-MTU-868688 native structured pathway source (source version: Reactome BioPAX 97)
PMID:23601637 Flux through Trehalose Synthase Flows from Trehalose to the Alpha Anomer of Maltose in Mycobacteria
UniProtKB:P9WFZ5 UniProtKB P9WFZ5 cofactor annotations (source version: 2026_03)

Curation history

2026-10-05T06:16:49Z Codex
- EDIT — Review complete causal graph against native sources and correct unsupported or omitted relationships
  All native sides, controllers, complexes, cofactors and cytosol locations inspected; source comments are not attributed as quotations from cited papers. Corrected TreS physiological direction and alpha-maltose product using PMID23601637. Every native record was inspected; see reports/causal-graph-diagram-review.json for native-object counts, exclusions and identity corrections. Semantic validation and native GraphId locator checks passed; authority snapshots and full corpus gates are coordinated separately.
Read this curation session