Sulfate assimilation

Reactome:R-MTU-936635

Mycobacterium tuberculosis sulfate uptake and activation links SubI binding, CysTWA transport, CysD/CysNC-dependent APS synthesis, APS phosphorylation and the CysQ PAPS-hydrolysis branch. GTP hydrolysis is coupled to APS synthesis, correcting its placement on APS kinase in Reactome. This record does not assert exclusive sulfate dependence, universal enzyme essentiality or complete stoichiometric balance. CysQ PAPS activity is qualitatively supported; PAP is its better-characterized substrate.

Pathway type

biopax pathway

Organism scope

Mycobacterium tuberculosis NCBITaxon:1773

Read the source YAML

Pathway network

33 nodes · 72 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

Show at full size (scroll to explore)

Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Mycobacterium tuberculosis `NCBITaxon:1773`	taxon
SO4(2-) `Reactome:R-ALL-427648`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
water `CHEBI:15377`	small molecule
ADP(3-) `CHEBI:456216`	small molecule
hydrogenphosphate `CHEBI:43474`	small molecule
SO4(2-) `Reactome:R-ALL-174375`	small molecule
5'-adenylyl sulfate(2-) `CHEBI:58243`	small molecule
diphosphate(3-) `CHEBI:33019`	small molecule
GTP(4-) `CHEBI:37565`	small molecule
hydron `CHEBI:15378`	small molecule
3'-phosphonato-5'-adenylyl sulfate(4-) `CHEBI:58339`	small molecule
GDP(3-) `CHEBI:58189`	small molecule
magnesium(2+) `CHEBI:18420`	cofactor
SubI `Reactome:R-MTU-936726`	protein
CysT `Reactome:R-MTU-936743`	protein
CysA `Reactome:R-MTU-936656`	protein
CysW `Reactome:R-MTU-936681`	protein
CysNC `Reactome:R-MTU-936597`	protein
CysD `Reactome:R-MTU-936698`	protein
CysQ `Reactome:R-MTU-936577`	protein
SubI--sulfate complex `Reactome:R-MTU-936589`	complex
sulfate ABC transporter `Reactome:R-MTU-936707`	complex
sulfate adenylyltransferase complex `Reactome:R-MTU-936606`	complex
CysQ dimer `Reactome:R-MTU-936691`	complex
CysQ-Mg++ `Reactome:R-MTU-936671`	complex
plasma membrane `GO:0005886`	cellular component
extracellular region `GO:0005576`	cellular component
cytosol `GO:0005829`	cellular component
sulfate binds to SubI outside the membrane `Reactome:R-MTU-936667`	molecular activity	left to right
sulfate uptake in the cytosol `Reactome:R-MTU-936631`	molecular activity	left to right
sulfate is activated to APS `Reactome:R-MTU-936729`	molecular activity	left to right
APS is phosphorylated to PAPS `Reactome:R-MTU-936583`	molecular activity	left to right
PAPS is dephosphorylated to APS `Reactome:R-MTU-936659`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
SubI `Reactome:R-MTU-936726`	consumes	sulfate binds to SubI outside the membrane `Reactome:R-MTU-936667`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction1 places physical entity Protein1 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction1/bp:left/#Protein1; #BiochemicalReaction1/bp:conversionDirection`
SO4(2-) `Reactome:R-ALL-427648`	consumes	sulfate binds to SubI outside the membrane `Reactome:R-MTU-936667`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction1 places physical entity SmallMolecule1 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction1/bp:left/#SmallMolecule1; #BiochemicalReaction1/bp:conversionDirection`
sulfate binds to SubI outside the membrane `Reactome:R-MTU-936667`	produces	SubI--sulfate complex `Reactome:R-MTU-936589`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction1 places physical entity Complex1 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction1/bp:right/#Complex1; #BiochemicalReaction1/bp:conversionDirection`
ATP(4-) `CHEBI:30616`	consumes	sulfate uptake in the cytosol `Reactome:R-MTU-936631`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction2 places physical entity SmallMolecule2 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction2/bp:left/#SmallMolecule2; #BiochemicalReaction2/bp:conversionDirection`
water `CHEBI:15377`	consumes	sulfate uptake in the cytosol `Reactome:R-MTU-936631`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction2 places physical entity SmallMolecule3 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction2/bp:left/#SmallMolecule3; #BiochemicalReaction2/bp:conversionDirection`
SubI--sulfate complex `Reactome:R-MTU-936589`	consumes	sulfate uptake in the cytosol `Reactome:R-MTU-936631`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction2 places physical entity Complex1 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction2/bp:left/#Complex1; #BiochemicalReaction2/bp:conversionDirection`
sulfate uptake in the cytosol `Reactome:R-MTU-936631`	produces	SubI `Reactome:R-MTU-936726`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction2 places physical entity Protein1 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction2/bp:right/#Protein1; #BiochemicalReaction2/bp:conversionDirection`
sulfate uptake in the cytosol `Reactome:R-MTU-936631`	produces	ADP(3-) `CHEBI:456216`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction2 places physical entity SmallMolecule4 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction2/bp:right/#SmallMolecule4; #BiochemicalReaction2/bp:conversionDirection`
sulfate uptake in the cytosol `Reactome:R-MTU-936631`	produces	hydrogenphosphate `CHEBI:43474`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction2 places physical entity SmallMolecule5 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction2/bp:right/#SmallMolecule5; #BiochemicalReaction2/bp:conversionDirection`
sulfate uptake in the cytosol `Reactome:R-MTU-936631`	produces	SO4(2-) `Reactome:R-ALL-174375`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction2 places physical entity SmallMolecule6 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction2/bp:right/#SmallMolecule6; #BiochemicalReaction2/bp:conversionDirection`
ATP(4-) `CHEBI:30616`	consumes	sulfate is activated to APS `Reactome:R-MTU-936729`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction3 places physical entity SmallMolecule2 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction3/bp:left/#SmallMolecule2; #BiochemicalReaction3/bp:conversionDirection`
SO4(2-) `Reactome:R-ALL-174375`	consumes	sulfate is activated to APS `Reactome:R-MTU-936729`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction3 places physical entity SmallMolecule6 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction3/bp:left/#SmallMolecule6; #BiochemicalReaction3/bp:conversionDirection`
sulfate is activated to APS `Reactome:R-MTU-936729`	produces	5'-adenylyl sulfate(2-) `CHEBI:58243`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction3 places physical entity SmallMolecule7 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction3/bp:right/#SmallMolecule7; #BiochemicalReaction3/bp:conversionDirection`
sulfate is activated to APS `Reactome:R-MTU-936729`	produces	diphosphate(3-) `CHEBI:33019`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction3 places physical entity SmallMolecule8 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction3/bp:right/#SmallMolecule8; #BiochemicalReaction3/bp:conversionDirection`
sulfate is activated to APS `Reactome:R-MTU-936729`	occurs_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: All physical entities on both sides of BioPAX BiochemicalReaction3 have the same cellular location. Source location `#BiochemicalReaction3/bp:left\|bp:right; physicalEntity/bp:cellularLocation/#CellularLocationVocabulary3`
ATP(4-) `CHEBI:30616`	consumes	APS is phosphorylated to PAPS `Reactome:R-MTU-936583`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction4 places physical entity SmallMolecule2 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction4/bp:left/#SmallMolecule2; #BiochemicalReaction4/bp:conversionDirection`
5'-adenylyl sulfate(2-) `CHEBI:58243`	consumes	APS is phosphorylated to PAPS `Reactome:R-MTU-936583`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction4 places physical entity SmallMolecule7 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction4/bp:left/#SmallMolecule7; #BiochemicalReaction4/bp:conversionDirection`
water `CHEBI:15377`	consumes Corrected placement of the GTP-hydrolysis component from APS kinase to the coupled APS-synthesis event. This qualitative edge does not specify a stoichiometric coefficient.	sulfate is activated to APS `Reactome:R-MTU-936729`	UniProtKB:P9WNM5 Source assertion: The structured FUNCTION annotation couples CysN GTP hydrolysis to CysD-mediated APS synthesis (ECO:0000250). This edge assigns the reactant CHEBI:15377 of that hydrolysis component to the APS-synthesis event. Source location `$.results[5].comments[0].texts[0]`
GTP(4-) `CHEBI:37565`	consumes Corrected placement of the GTP-hydrolysis component from APS kinase to the coupled APS-synthesis event. This qualitative edge does not specify a stoichiometric coefficient.	sulfate is activated to APS `Reactome:R-MTU-936729`	UniProtKB:P9WNM5 Source assertion: The structured FUNCTION annotation couples CysN GTP hydrolysis to CysD-mediated APS synthesis (ECO:0000250). This edge assigns the reactant CHEBI:37565 of that hydrolysis component to the APS-synthesis event. Source location `$.results[5].comments[0].texts[0]` PMID:15615729 The stoichiometry of GTP hydrolysis and APS synthesis is 1:1
APS is phosphorylated to PAPS `Reactome:R-MTU-936583`	produces	ADP(3-) `CHEBI:456216`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction4 places physical entity SmallMolecule4 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction4/bp:right/#SmallMolecule4; #BiochemicalReaction4/bp:conversionDirection`
APS is phosphorylated to PAPS `Reactome:R-MTU-936583`	produces Qualitative hydron product supported by the APS kinase reaction. Reactome source multiplicity of two is not retained. APS-synthesis proton consumption and coupled GTP-hydrolysis proton production are netted; this graph has no explicit stoichiometric model.	hydron `CHEBI:15378`	UniProtKB:P9WNM5 Source assertion: The CATALYTIC ACTIVITY reaction for EC:2.7.1.25 lists APS and ATP as reactants and PAPS, ADP and H(+) as products (RHEA:24152). Source location `$.results[5].comments[3].reaction`
sulfate is activated to APS `Reactome:R-MTU-936729`	produces Corrected placement of the GTP-hydrolysis component from APS kinase to the coupled APS-synthesis event. This qualitative edge does not specify a stoichiometric coefficient.	hydrogenphosphate `CHEBI:43474`	UniProtKB:P9WNM5 Source assertion: The structured FUNCTION annotation couples CysN GTP hydrolysis to CysD-mediated APS synthesis (ECO:0000250). This edge assigns the product CHEBI:43474 of that hydrolysis component to the APS-synthesis event. Source location `$.results[5].comments[0].texts[0]`
APS is phosphorylated to PAPS `Reactome:R-MTU-936583`	produces	3'-phosphonato-5'-adenylyl sulfate(4-) `CHEBI:58339`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction4 places physical entity SmallMolecule11 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction4/bp:right/#SmallMolecule11; #BiochemicalReaction4/bp:conversionDirection`
sulfate is activated to APS `Reactome:R-MTU-936729`	produces Corrected placement of the GTP-hydrolysis component from APS kinase to the coupled APS-synthesis event. This qualitative edge does not specify a stoichiometric coefficient.	GDP(3-) `CHEBI:58189`	UniProtKB:P9WNM5 Source assertion: The structured FUNCTION annotation couples CysN GTP hydrolysis to CysD-mediated APS synthesis (ECO:0000250). This edge assigns the product CHEBI:58189 of that hydrolysis component to the APS-synthesis event. Source location `$.results[5].comments[0].texts[0]`
APS is phosphorylated to PAPS `Reactome:R-MTU-936583`	occurs_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: All physical entities on both sides of BioPAX BiochemicalReaction4 have the same cellular location. Source location `#BiochemicalReaction4/bp:left\|bp:right; physicalEntity/bp:cellularLocation/#CellularLocationVocabulary3`
water `CHEBI:15377`	consumes	PAPS is dephosphorylated to APS `Reactome:R-MTU-936659`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction5 places physical entity SmallMolecule3 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction5/bp:left/#SmallMolecule3; #BiochemicalReaction5/bp:conversionDirection`
3'-phosphonato-5'-adenylyl sulfate(4-) `CHEBI:58339`	consumes	PAPS is dephosphorylated to APS `Reactome:R-MTU-936659`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction5 places physical entity SmallMolecule11 on its left side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction5/bp:left/#SmallMolecule11; #BiochemicalReaction5/bp:conversionDirection`
PAPS is dephosphorylated to APS `Reactome:R-MTU-936659`	produces	5'-adenylyl sulfate(2-) `CHEBI:58243`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction5 places physical entity SmallMolecule7 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction5/bp:right/#SmallMolecule7; #BiochemicalReaction5/bp:conversionDirection`
PAPS is dephosphorylated to APS `Reactome:R-MTU-936659`	produces	hydrogenphosphate `CHEBI:43474`	Reactome:R-MTU-936635 Source assertion: BioPAX BiochemicalReaction5 places physical entity SmallMolecule5 on its right side; conversion direction is LEFT-TO-RIGHT; displayed orientation is LEFT-TO-RIGHT. Source location `#BiochemicalReaction5/bp:right/#SmallMolecule5; #BiochemicalReaction5/bp:conversionDirection`
PAPS is dephosphorylated to APS `Reactome:R-MTU-936659`	occurs_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: All physical entities on both sides of BioPAX BiochemicalReaction5 have the same cellular location. Source location `#BiochemicalReaction5/bp:left\|bp:right; physicalEntity/bp:cellularLocation/#CellularLocationVocabulary3`
sulfate ABC transporter `Reactome:R-MTU-936707`	catalyzes	sulfate uptake in the cytosol `Reactome:R-MTU-936631`	Reactome:R-MTU-936635 Source assertion: BioPAX Catalysis1 names Complex2 as its catalytic controller. Source location `#Catalysis1/bp:controller/#Complex2; bp:controlled/#BiochemicalReaction2`
sulfate adenylyltransferase complex `Reactome:R-MTU-936606`	catalyzes	sulfate is activated to APS `Reactome:R-MTU-936729`	Reactome:R-MTU-936635 Source assertion: BioPAX Catalysis2 names Complex3 as its catalytic controller. Source location `#Catalysis2/bp:controller/#Complex3; bp:controlled/#BiochemicalReaction3`
sulfate adenylyltransferase complex `Reactome:R-MTU-936606`	catalyzes	APS is phosphorylated to PAPS `Reactome:R-MTU-936583`	Reactome:R-MTU-936635 Source assertion: BioPAX Catalysis3 names Complex3 as its catalytic controller. Source location `#Catalysis3/bp:controller/#Complex3; bp:controlled/#BiochemicalReaction4`
CysQ dimer `Reactome:R-MTU-936691`	catalyzes Source CysQ PAPS hydrolysis assignment has qualitative biochemical support (PMID18454554). PAP, not PAPS, was quantitatively characterized; membrane localization and oligomer context are retained as Reactome assertions.	PAPS is dephosphorylated to APS `Reactome:R-MTU-936659`	Reactome:R-MTU-936635 Source assertion: BioPAX Catalysis4 names Complex4 as its catalytic controller. Source location `#Catalysis4/bp:controller/#Complex4; bp:controlled/#BiochemicalReaction5`
GTP(4-) `CHEBI:37565`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule9 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule9/bp:cellularLocation/#CellularLocationVocabulary3`
diphosphate(3-) `CHEBI:33019`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule8 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule8/bp:cellularLocation/#CellularLocationVocabulary3`
5'-adenylyl sulfate(2-) `CHEBI:58243`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule7 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule7/bp:cellularLocation/#CellularLocationVocabulary3`
SO4(2-) `Reactome:R-ALL-174375`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule6 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule6/bp:cellularLocation/#CellularLocationVocabulary3`
hydrogenphosphate `CHEBI:43474`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule5 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule5/bp:cellularLocation/#CellularLocationVocabulary3`
ADP(3-) `CHEBI:456216`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule4 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule4/bp:cellularLocation/#CellularLocationVocabulary3`
water `CHEBI:15377`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule3 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule3/bp:cellularLocation/#CellularLocationVocabulary3`
ATP(4-) `CHEBI:30616`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule2 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule2/bp:cellularLocation/#CellularLocationVocabulary3`
GDP(3-) `CHEBI:58189`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule12 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule12/bp:cellularLocation/#CellularLocationVocabulary3`
3'-phosphonato-5'-adenylyl sulfate(4-) `CHEBI:58339`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule11 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule11/bp:cellularLocation/#CellularLocationVocabulary3`
hydron `CHEBI:15378`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule10 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule10/bp:cellularLocation/#CellularLocationVocabulary3`
SO4(2-) `Reactome:R-ALL-427648`	located_in	extracellular region `GO:0005576`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule1 is assigned to cellular location CellularLocationVocabulary2. Source location `#SmallMolecule1/bp:cellularLocation/#CellularLocationVocabulary2`
SubI `Reactome:R-MTU-936726`	located_in	plasma membrane `GO:0005886`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Protein1 is assigned to cellular location CellularLocationVocabulary1. Source location `#Protein1/bp:cellularLocation/#CellularLocationVocabulary1`
CysQ dimer `Reactome:R-MTU-936691`	has_part	CysQ-Mg++ `Reactome:R-MTU-936671`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex4 explicitly includes physical entity Complex5. Source location `#Complex4/bp:component/#Complex5`
CysQ dimer `Reactome:R-MTU-936691`	located_in	plasma membrane `GO:0005886`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Complex4 is assigned to cellular location CellularLocationVocabulary1. Source location `#Complex4/bp:cellularLocation/#CellularLocationVocabulary1`
CysQ-Mg++ `Reactome:R-MTU-936671`	has_part	magnesium(2+) `CHEBI:18420`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex5 explicitly includes physical entity SmallMolecule13. Source location `#Complex5/bp:component/#SmallMolecule13`
CysQ-Mg++ `Reactome:R-MTU-936671`	has_part	CysQ `Reactome:R-MTU-936577`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex5 explicitly includes physical entity Protein7. Source location `#Complex5/bp:component/#Protein7`
CysQ-Mg++ `Reactome:R-MTU-936671`	located_in	plasma membrane `GO:0005886`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Complex5 is assigned to cellular location CellularLocationVocabulary1. Source location `#Complex5/bp:cellularLocation/#CellularLocationVocabulary1`
CysQ `Reactome:R-MTU-936577`	located_in	plasma membrane `GO:0005886`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Protein7 is assigned to cellular location CellularLocationVocabulary1. Source location `#Protein7/bp:cellularLocation/#CellularLocationVocabulary1`
magnesium(2+) `CHEBI:18420`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity SmallMolecule13 is assigned to cellular location CellularLocationVocabulary3. Source location `#SmallMolecule13/bp:cellularLocation/#CellularLocationVocabulary3`
sulfate adenylyltransferase complex `Reactome:R-MTU-936606`	has_part	CysNC `Reactome:R-MTU-936597`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex3 explicitly includes physical entity Protein5. Source location `#Complex3/bp:component/#Protein5`
sulfate adenylyltransferase complex `Reactome:R-MTU-936606`	has_part	CysD `Reactome:R-MTU-936698`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex3 explicitly includes physical entity Protein6. Source location `#Complex3/bp:component/#Protein6`
sulfate adenylyltransferase complex `Reactome:R-MTU-936606`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Complex3 is assigned to cellular location CellularLocationVocabulary3. Source location `#Complex3/bp:cellularLocation/#CellularLocationVocabulary3`
CysD `Reactome:R-MTU-936698`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Protein6 is assigned to cellular location CellularLocationVocabulary3. Source location `#Protein6/bp:cellularLocation/#CellularLocationVocabulary3`
CysNC `Reactome:R-MTU-936597`	located_in	cytosol `GO:0005829`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Protein5 is assigned to cellular location CellularLocationVocabulary3. Source location `#Protein5/bp:cellularLocation/#CellularLocationVocabulary3`
sulfate ABC transporter `Reactome:R-MTU-936707`	has_part	CysT `Reactome:R-MTU-936743`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex2 explicitly includes physical entity Protein2. Source location `#Complex2/bp:component/#Protein2`
sulfate ABC transporter `Reactome:R-MTU-936707`	has_part	CysA `Reactome:R-MTU-936656`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex2 explicitly includes physical entity Protein3. Source location `#Complex2/bp:component/#Protein3`
sulfate ABC transporter `Reactome:R-MTU-936707`	has_part	CysW `Reactome:R-MTU-936681`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex2 explicitly includes physical entity Protein4. Source location `#Complex2/bp:component/#Protein4`
sulfate ABC transporter `Reactome:R-MTU-936707`	located_in	plasma membrane `GO:0005886`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Complex2 is assigned to cellular location CellularLocationVocabulary1. Source location `#Complex2/bp:cellularLocation/#CellularLocationVocabulary1`
CysW `Reactome:R-MTU-936681`	located_in	plasma membrane `GO:0005886`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Protein4 is assigned to cellular location CellularLocationVocabulary1. Source location `#Protein4/bp:cellularLocation/#CellularLocationVocabulary1`
CysA `Reactome:R-MTU-936656`	located_in	plasma membrane `GO:0005886`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Protein3 is assigned to cellular location CellularLocationVocabulary1. Source location `#Protein3/bp:cellularLocation/#CellularLocationVocabulary1`
CysT `Reactome:R-MTU-936743`	located_in	plasma membrane `GO:0005886`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Protein2 is assigned to cellular location CellularLocationVocabulary1. Source location `#Protein2/bp:cellularLocation/#CellularLocationVocabulary1`
SubI--sulfate complex `Reactome:R-MTU-936589`	has_part	SubI `Reactome:R-MTU-936726`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex1 explicitly includes physical entity Protein1. Source location `#Complex1/bp:component/#Protein1`
SubI--sulfate complex `Reactome:R-MTU-936589`	has_part	SO4(2-) `Reactome:R-ALL-427648`	Reactome:R-MTU-936635 Source assertion: BioPAX complex Complex1 explicitly includes physical entity SmallMolecule1. Source location `#Complex1/bp:component/#SmallMolecule1`
SubI--sulfate complex `Reactome:R-MTU-936589`	located_in	plasma membrane `GO:0005886`	Reactome:R-MTU-936635 Source assertion: BioPAX physical entity Complex1 is assigned to cellular location CellularLocationVocabulary1. Source location `#Complex1/bp:cellularLocation/#CellularLocationVocabulary1`
sulfate binds to SubI outside the membrane `Reactome:R-MTU-936667`	provides_input_for Source-ordered material transfer through Reactome:R-MTU-936589; no regulatory sign or stoichiometric coefficient is asserted.	sulfate uptake in the cytosol `Reactome:R-MTU-936631`	Reactome:R-MTU-936635 Source assertion: BioPAX PathwayStep1 explicitly lists PathwayStep2 as nextStep; the linked reactions produce and consume the same source physical entity. Source location `#PathwayStep1/bp:nextStep/#PathwayStep2; #BiochemicalReaction1/bp:right/#Complex1; #BiochemicalReaction1/bp:conversionDirection; #BiochemicalReaction2/bp:left/#Complex1; #BiochemicalReaction2/bp:conversionDirection`
sulfate uptake in the cytosol `Reactome:R-MTU-936631`	provides_input_for Source-ordered material transfer through Reactome:R-ALL-174375; no regulatory sign or stoichiometric coefficient is asserted.	sulfate is activated to APS `Reactome:R-MTU-936729`	Reactome:R-MTU-936635 Source assertion: BioPAX PathwayStep2 explicitly lists PathwayStep3 as nextStep; the linked reactions produce and consume the same source physical entity. Source location `#PathwayStep2/bp:nextStep/#PathwayStep3; #BiochemicalReaction2/bp:right/#SmallMolecule6; #BiochemicalReaction2/bp:conversionDirection; #BiochemicalReaction3/bp:left/#SmallMolecule6; #BiochemicalReaction3/bp:conversionDirection`
sulfate is activated to APS `Reactome:R-MTU-936729`	provides_input_for Source-ordered material transfer through CHEBI:58243; no regulatory sign or stoichiometric coefficient is asserted.	APS is phosphorylated to PAPS `Reactome:R-MTU-936583`	Reactome:R-MTU-936635 Source assertion: BioPAX PathwayStep3 explicitly lists PathwayStep4 as nextStep; the linked reactions produce and consume the same source physical entity. Source location `#PathwayStep3/bp:nextStep/#PathwayStep4; #BiochemicalReaction3/bp:right/#SmallMolecule7; #BiochemicalReaction3/bp:conversionDirection; #BiochemicalReaction4/bp:left/#SmallMolecule7; #BiochemicalReaction4/bp:conversionDirection`

References

PMID:11929522 Functional genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivo
PMID:15184554 The Mycobacterium tuberculosis cysD and cysNC genes form a stress-induced operon that encodes a tri-functional sulfate-activating complex
PMID:15615729 The trifunctional sulfate-activating complex (SAC) of Mycobacterium tuberculosis
PMID:18454554 Rv2131c from Mycobacterium tuberculosis is a CysQ 3'-phosphoadenosine-5'-phosphatase
PMID:17970225 Drug targets in mycobacterial sulfur metabolism
Reactome:R-MTU-936635 BioPAX source pathway Reactome:R-MTU-936635 (source version: Reactome release 97)
UniProtKB:P9WNM5 UniProtKB P9WNM5 bifunctional CysNC function and catalytic-activity annotations (source version: retrieved 2026-10-07; release not exposed)

Curation history

2026-10-07T07:37:27Z Codex
- CREATE — Curate Reactome:R-MTU-936635 with native physical states and reviewed chemistry
  Added 5 reactions and 72 edges from hash-pinned Reactome release 97. Complete source projection and edge-disposition ledger are in research/source_discovery/2026-10-07-reactome. Preserve modified, redox and cleaved protein states; exclude unsupported sulfur charging and CysK2/OAS chemistry where relevant; correct GTP coupling in sulfate activation. Exact source and primary-evidence limitations are explicit. Native and closed-schema validation passed before writing. Hidden/ignored-file deduplication included.
Read this curation session