Isoleucine degradation

WikiPathways:WP178

Isoleucine degradation converts L-isoleucine to (S)-3-methyl-2-oxopentanoate, 2-methylbutanal, and 2-methylbutanol in Saccharomyces cerevisiae.

Pathway type

amino acid degradation

Organism scope

Saccharomyces cerevisiae NCBITaxon:4932

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Pathway network

27 nodes · 35 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae `NCBITaxon:4932`	taxon
L-isoleucine `CHEBI:58045`	small molecule
BAT2 `SGD:S000003909`	protein
2-methylbutanal `CHEBI:16182`	small molecule
NAD(1-) `CHEBI:57540`	small molecule
BAT1 `SGD:S000001251`	protein
NADH(2-) `CHEBI:57945`	small molecule
PDC1 `SGD:S000004034`	protein
L-glutamate(1-) `CHEBI:29985`	small molecule
ARO10 `SGD:S000002788`	protein
PDC6 `SGD:S000003319`	protein
hydron `CHEBI:15378`	small molecule
2-oxoglutarate(2-) `CHEBI:16810`	small molecule
PDC5 `SGD:S000004124`	protein
2-methylbutanol `CHEBI:48945`	small molecule
SFA1 `SGD:S000002327`	protein
carbon dioxide `CHEBI:16526`	small molecule
(S)-3-methyl-2-oxopentanoate `CHEBI:35146`	small molecule
ADH5 `SGD:S000000349`	protein
ADH4 `SGD:S000003225`	protein
pyridoxal 5'-phosphate(2-) `CHEBI:597326`	cofactor
magnesium(2+) `CHEBI:18420`	cofactor
thiamine(1+) diphosphate(3-) `CHEBI:58937`	cofactor
zinc(2+) `CHEBI:29105`	cofactor
iron(2+) `CHEBI:29033`	cofactor
L-isoleucine to (S)-3-methyl-2-oxopentanoate GPML interaction `WikiPathways:WP178/id59c25f71`	molecular activity	left to right
2-methylbutanal to 2-methylbutanol GPML interaction `WikiPathways:WP178/idbcd10869`	molecular activity	left to right
(S)-3-methyl-2-oxopentanoate to 2-methylbutanal GPML interaction `WikiPathways:WP178/idf1c4befb`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
L-isoleucine `CHEBI:58045`	consumes	L-isoleucine to (S)-3-methyl-2-oxopentanoate GPML interaction `WikiPathways:WP178/id59c25f71`	WikiPathways:WP178 Source assertion: GPML id59c25f71 connects normalized CHEBI:58045 as an input of interaction id59c25f71. Source location `/Pathway/Interaction[@GraphId='id59c25f71']/Graphics; /Pathway/DataNode[@GraphId='a702d']`
L-isoleucine to (S)-3-methyl-2-oxopentanoate GPML interaction `WikiPathways:WP178/id59c25f71`	produces	(S)-3-methyl-2-oxopentanoate `CHEBI:35146`	WikiPathways:WP178 Source assertion: GPML id59c25f71 connects normalized CHEBI:35146 as an output of interaction id59c25f71. Source location `/Pathway/Interaction[@GraphId='id59c25f71']/Graphics; /Pathway/DataNode[@GraphId='f7ede']`
2-methylbutanal `CHEBI:16182`	consumes	2-methylbutanal to 2-methylbutanol GPML interaction `WikiPathways:WP178/idbcd10869`	WikiPathways:WP178 Source assertion: GPML idbcd10869 connects normalized CHEBI:16182 as an input of interaction idbcd10869. Source location `/Pathway/Interaction[@GraphId='idbcd10869']/Graphics; /Pathway/DataNode[@GraphId='ae17b']`
2-methylbutanal to 2-methylbutanol GPML interaction `WikiPathways:WP178/idbcd10869`	produces	2-methylbutanol `CHEBI:48945`	WikiPathways:WP178 Source assertion: GPML idbcd10869 connects normalized CHEBI:48945 as an output of interaction idbcd10869. Source location `/Pathway/Interaction[@GraphId='idbcd10869']/Graphics; /Pathway/DataNode[@GraphId='ec74f']`
(S)-3-methyl-2-oxopentanoate `CHEBI:35146`	consumes	(S)-3-methyl-2-oxopentanoate to 2-methylbutanal GPML interaction `WikiPathways:WP178/idf1c4befb`	WikiPathways:WP178 Source assertion: GPML idf1c4befb connects normalized CHEBI:35146 as an input of interaction idf1c4befb. Source location `/Pathway/Interaction[@GraphId='idf1c4befb']/Graphics; /Pathway/DataNode[@GraphId='f7ede']`
(S)-3-methyl-2-oxopentanoate to 2-methylbutanal GPML interaction `WikiPathways:WP178/idf1c4befb`	produces	2-methylbutanal `CHEBI:16182`	WikiPathways:WP178 Source assertion: GPML idf1c4befb connects normalized CHEBI:16182 as an output of interaction idf1c4befb. Source location `/Pathway/Interaction[@GraphId='idf1c4befb']/Graphics; /Pathway/DataNode[@GraphId='ae17b']`
(S)-3-methyl-2-oxopentanoate to 2-methylbutanal GPML interaction `WikiPathways:WP178/idf1c4befb`	produces	carbon dioxide `CHEBI:16526`	WikiPathways:WP178 Source assertion: GPML id122c722 connects normalized CHEBI:16526 as an output of interaction idf1c4befb. Source location `/Pathway/Interaction[@GraphId='id122c722']/Graphics; /Pathway/DataNode[@GraphId='f1acf']`
SFA1 `SGD:S000002327`	catalyzes	2-methylbutanal to 2-methylbutanol GPML interaction `WikiPathways:WP178/idbcd10869`	WikiPathways:WP178 Source assertion: GPML id3c6e83b4 connects normalized SGD:S000002327 as a catalyst of interaction idbcd10869. Source location `/Pathway/Interaction[@GraphId='id3c6e83b4']/Graphics; /Pathway/DataNode[@GraphId='ef0ef']`
ADH5 `SGD:S000000349`	catalyzes	2-methylbutanal to 2-methylbutanol GPML interaction `WikiPathways:WP178/idbcd10869`	WikiPathways:WP178 Source assertion: GPML id3c6e83b4 connects normalized SGD:S000000349 as a catalyst of interaction idbcd10869. Source location `/Pathway/Interaction[@GraphId='id3c6e83b4']/Graphics; /Pathway/DataNode[@GraphId='afbec']`
ADH4 `SGD:S000003225`	catalyzes	2-methylbutanal to 2-methylbutanol GPML interaction `WikiPathways:WP178/idbcd10869`	WikiPathways:WP178 Source assertion: GPML id3c6e83b4 connects normalized SGD:S000003225 as a catalyst of interaction idbcd10869. Source location `/Pathway/Interaction[@GraphId='id3c6e83b4']/Graphics; /Pathway/DataNode[@GraphId='ee6f3']`
BAT2 `SGD:S000003909`	catalyzes	L-isoleucine to (S)-3-methyl-2-oxopentanoate GPML interaction `WikiPathways:WP178/id59c25f71`	WikiPathways:WP178 Source assertion: GPML id6ce772fa connects normalized SGD:S000003909 as a catalyst of interaction id59c25f71. Source location `/Pathway/Interaction[@GraphId='id6ce772fa']/Graphics; /Pathway/DataNode[@GraphId='a9a']`
BAT1 `SGD:S000001251`	catalyzes	L-isoleucine to (S)-3-methyl-2-oxopentanoate GPML interaction `WikiPathways:WP178/id59c25f71`	WikiPathways:WP178 Source assertion: GPML id6ce772fa connects normalized SGD:S000001251 as a catalyst of interaction id59c25f71. Source location `/Pathway/Interaction[@GraphId='id6ce772fa']/Graphics; /Pathway/DataNode[@GraphId='b88']`
NADH(2-) `CHEBI:57945`	consumes	2-methylbutanal to 2-methylbutanol GPML interaction `WikiPathways:WP178/idbcd10869`	WikiPathways:WP178 Source assertion: GPML id6d78e0dd connects normalized CHEBI:57945 as an input of interaction idbcd10869. Source location `/Pathway/Interaction[@GraphId='id6d78e0dd']/Graphics; /Pathway/DataNode[@GraphId='bc33d']`
2-methylbutanal to 2-methylbutanol GPML interaction `WikiPathways:WP178/idbcd10869`	produces	NAD(1-) `CHEBI:57540`	WikiPathways:WP178 Source assertion: GPML id7aa61fb4 connects normalized CHEBI:57540 as an output of interaction idbcd10869. Source location `/Pathway/Interaction[@GraphId='id7aa61fb4']/Graphics; /Pathway/DataNode[@GraphId='b6044']`
PDC1 `SGD:S000004034`	catalyzes	(S)-3-methyl-2-oxopentanoate to 2-methylbutanal GPML interaction `WikiPathways:WP178/idf1c4befb`	WikiPathways:WP178 Source assertion: GPML id88f86b02 connects normalized SGD:S000004034 as a catalyst of interaction idf1c4befb. Source location `/Pathway/Interaction[@GraphId='id88f86b02']/Graphics; /Pathway/DataNode[@GraphId='beb']`
ARO10 `SGD:S000002788`	catalyzes	(S)-3-methyl-2-oxopentanoate to 2-methylbutanal GPML interaction `WikiPathways:WP178/idf1c4befb`	WikiPathways:WP178 Source assertion: GPML id88f86b02 connects normalized SGD:S000002788 as a catalyst of interaction idf1c4befb. Source location `/Pathway/Interaction[@GraphId='id88f86b02']/Graphics; /Pathway/DataNode[@GraphId='d89']`
PDC6 `SGD:S000003319`	catalyzes	(S)-3-methyl-2-oxopentanoate to 2-methylbutanal GPML interaction `WikiPathways:WP178/idf1c4befb`	WikiPathways:WP178 Source assertion: GPML id88f86b02 connects normalized SGD:S000003319 as a catalyst of interaction idf1c4befb. Source location `/Pathway/Interaction[@GraphId='id88f86b02']/Graphics; /Pathway/DataNode[@GraphId='da5']`
PDC5 `SGD:S000004124`	catalyzes	(S)-3-methyl-2-oxopentanoate to 2-methylbutanal GPML interaction `WikiPathways:WP178/idf1c4befb`	WikiPathways:WP178 Source assertion: GPML id88f86b02 connects normalized SGD:S000004124 as a catalyst of interaction idf1c4befb. Source location `/Pathway/Interaction[@GraphId='id88f86b02']/Graphics; /Pathway/DataNode[@GraphId='e36']`
2-oxoglutarate(2-) `CHEBI:16810`	consumes	L-isoleucine to (S)-3-methyl-2-oxopentanoate GPML interaction `WikiPathways:WP178/id59c25f71`	WikiPathways:WP178 Source assertion: GPML idb65db85 connects normalized CHEBI:16810 as an input of interaction id59c25f71. Source location `/Pathway/Interaction[@GraphId='idb65db85']/Graphics; /Pathway/DataNode[@GraphId='e2f2a']`
L-isoleucine to (S)-3-methyl-2-oxopentanoate GPML interaction `WikiPathways:WP178/id59c25f71`	produces	L-glutamate(1-) `CHEBI:29985`	WikiPathways:WP178 Source assertion: GPML idba75f623 connects normalized CHEBI:29985 as an output of interaction id59c25f71. Source location `/Pathway/Interaction[@GraphId='idba75f623']/Graphics; /Pathway/DataNode[@GraphId='d5f']`
hydron `CHEBI:15378`	consumes	(S)-3-methyl-2-oxopentanoate to 2-methylbutanal GPML interaction `WikiPathways:WP178/idf1c4befb`	WikiPathways:WP178 Source assertion: GPML ide5cba93b connects normalized CHEBI:15378 as an input of interaction idf1c4befb. Source location `/Pathway/Interaction[@GraphId='ide5cba93b']/Graphics; /Pathway/DataNode[@GraphId='e284e']`
BAT2 `SGD:S000003909`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P47176 Source assertion: UniProtKB:P47176 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1987/comments/0/cofactors/0` UniProtKB:P47176 Source assertion: UniProtKB:P47176 has the exact SGD cross-reference SGD:S000003909. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1987/uniProtKBCrossReferences/0`
BAT1 `SGD:S000001251`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. Reviewed source qualification: Disputed growth phenotypes do not address PLP binding; retain the similarity-based PLP annotation. UniProt evidence: ECO:0000250 (UniProtKB:P0AB80)	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P38891 Source assertion: UniProtKB:P38891 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1497/comments/0/cofactors/0` UniProtKB:P38891 Source assertion: UniProtKB:P38891 has the exact SGD cross-reference SGD:S000001251. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1497/uniProtKBCrossReferences/0`
PDC1 `SGD:S000004034`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:10651824) Binds 1 Mg(2+) per subunit. UniProt note evidence: ECO:0000269 (PubMed:10651824)	magnesium(2+) `CHEBI:18420`	UniProtKB:P06169 Source assertion: UniProtKB:P06169 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/127/comments/0/cofactors/0; /results/127/comments/0/note` UniProtKB:P06169 Source assertion: UniProtKB:P06169 has the exact SGD cross-reference SGD:S000004034. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/127/uniProtKBCrossReferences/0`
PDC1 `SGD:S000004034`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:10651824) Binds 1 thiamine pyrophosphate per subunit. UniProt note evidence: ECO:0000269 (PubMed:10651824)	thiamine(1+) diphosphate(3-) `CHEBI:58937`	UniProtKB:P06169 Source assertion: UniProtKB:P06169 annotates CHEBI:58937 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/127/comments/1/cofactors/0; /results/127/comments/1/note` UniProtKB:P06169 Source assertion: UniProtKB:P06169 has the exact SGD cross-reference SGD:S000004034. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/127/uniProtKBCrossReferences/0`
ARO10 `SGD:S000002788`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 Mg(2+) per subunit. UniProt note evidence: ECO:0000250	magnesium(2+) `CHEBI:18420`	UniProtKB:Q06408 Source assertion: UniProtKB:Q06408 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2752/comments/0/cofactors/0; /results/2752/comments/0/note` UniProtKB:Q06408 Source assertion: UniProtKB:Q06408 has the exact SGD cross-reference SGD:S000002788. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2752/uniProtKBCrossReferences/0`
ARO10 `SGD:S000002788`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 thiamine pyrophosphate per subunit. UniProt note evidence: ECO:0000250	thiamine(1+) diphosphate(3-) `CHEBI:58937`	UniProtKB:Q06408 Source assertion: UniProtKB:Q06408 annotates CHEBI:58937 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2752/comments/1/cofactors/0; /results/2752/comments/1/note` UniProtKB:Q06408 Source assertion: UniProtKB:Q06408 has the exact SGD cross-reference SGD:S000002788. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2752/uniProtKBCrossReferences/0`
PDC6 `SGD:S000003319`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P06169) Binds 1 Mg(2+) per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P06169)	magnesium(2+) `CHEBI:18420`	UniProtKB:P26263 Source assertion: UniProtKB:P26263 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/742/comments/0/cofactors/0; /results/742/comments/0/note` UniProtKB:P26263 Source assertion: UniProtKB:P26263 has the exact SGD cross-reference SGD:S000003319. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/742/uniProtKBCrossReferences/0`
PDC6 `SGD:S000003319`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P06169) Binds 1 thiamine pyrophosphate per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P06169)	thiamine(1+) diphosphate(3-) `CHEBI:58937`	UniProtKB:P26263 Source assertion: UniProtKB:P26263 annotates CHEBI:58937 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/742/comments/1/cofactors/0; /results/742/comments/1/note` UniProtKB:P26263 Source assertion: UniProtKB:P26263 has the exact SGD cross-reference SGD:S000003319. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/742/uniProtKBCrossReferences/0`
PDC5 `SGD:S000004124`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P06169) Binds 1 Mg(2+) per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P06169)	magnesium(2+) `CHEBI:18420`	UniProtKB:P16467 Source assertion: UniProtKB:P16467 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/439/comments/0/cofactors/0; /results/439/comments/0/note` UniProtKB:P16467 Source assertion: UniProtKB:P16467 has the exact SGD cross-reference SGD:S000004124. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/439/uniProtKBCrossReferences/0`
PDC5 `SGD:S000004124`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P06169) Binds 1 thiamine pyrophosphate per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P06169)	thiamine(1+) diphosphate(3-) `CHEBI:58937`	UniProtKB:P16467 Source assertion: UniProtKB:P16467 annotates CHEBI:58937 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/439/comments/1/cofactors/0; /results/439/comments/1/note` UniProtKB:P16467 Source assertion: UniProtKB:P16467 has the exact SGD cross-reference SGD:S000004124. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/439/uniProtKBCrossReferences/0`
SFA1 `SGD:S000002327`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P06525) Binds 2 Zn(2+) ions per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P06525)	zinc(2+) `CHEBI:29105`	UniProtKB:P32771 Source assertion: UniProtKB:P32771 annotates CHEBI:29105 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/990/comments/0/cofactors/0; /results/990/comments/0/note` UniProtKB:P32771 Source assertion: UniProtKB:P32771 has the exact SGD cross-reference SGD:S000002327. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/990/uniProtKBCrossReferences/0`
ADH5 `SGD:S000000349`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P00330) Binds 2 Zn(2+) ions per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P00330)	zinc(2+) `CHEBI:29105`	UniProtKB:P38113 Source assertion: UniProtKB:P38113 annotates CHEBI:29105 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3563/comments/0/cofactors/0; /results/3563/comments/0/note` UniProtKB:P38113 Source assertion: UniProtKB:P38113 has the exact SGD cross-reference SGD:S000000349. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3563/uniProtKBCrossReferences/0`
ADH4 `SGD:S000003225`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:3282541) Zinc. May bind iron when zinc levels are limiting. UniProt note evidence: ECO:0000269 (PubMed:3282541)	zinc(2+) `CHEBI:29105`	UniProtKB:P10127 Source assertion: UniProtKB:P10127 annotates CHEBI:29105 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/302/comments/0/cofactors/0; /results/302/comments/0/note` UniProtKB:P10127 Source assertion: UniProtKB:P10127 has the exact SGD cross-reference SGD:S000003225. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/302/uniProtKBCrossReferences/0`
ADH4 `SGD:S000003225`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:3282541) Zinc. May bind iron when zinc levels are limiting. UniProt note evidence: ECO:0000269 (PubMed:3282541)	iron(2+) `CHEBI:29033`	UniProtKB:P10127 Source assertion: UniProtKB:P10127 annotates CHEBI:29033 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/302/comments/0/cofactors/1; /results/302/comments/0/note` UniProtKB:P10127 Source assertion: UniProtKB:P10127 has the exact SGD cross-reference SGD:S000003225. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/302/uniProtKBCrossReferences/0`

References

WikiPathways:WP178 WP178 native structured pathway source (source version: WP178_r140283)
DOI:10.1128/AEM.01675-12 Substrate Specificity of Thiamine Pyrophosphate-Dependent 2-Oxo-Acid Decarboxylases in Saccharomyces cerevisiae
UniProtKB:P47176 UniProtKB P47176 cofactor annotations (source version: 2026_03)
UniProtKB:P38891 UniProtKB P38891 cofactor annotations (source version: 2026_03)
UniProtKB:P06169 UniProtKB P06169 cofactor annotations (source version: 2026_03)
UniProtKB:Q06408 UniProtKB Q06408 cofactor annotations (source version: 2026_03)
UniProtKB:P26263 UniProtKB P26263 cofactor annotations (source version: 2026_03)
UniProtKB:P16467 UniProtKB P16467 cofactor annotations (source version: 2026_03)
UniProtKB:P32771 UniProtKB P32771 cofactor annotations (source version: 2026_03)
UniProtKB:P38113 UniProtKB P38113 cofactor annotations (source version: 2026_03)
UniProtKB:P10127 UniProtKB P10127 cofactor annotations (source version: 2026_03)

Curation history

2026-10-05T06:16:43Z Codex
- EDIT — Review complete causal graph against native sources and correct unsupported or omitted relationships
  Restored catalysts and cosubstrates. Excluded THI3 catalytic assignment: individual enzyme assays in DOI10.1128/AEM.01675-12 found no activity. No subcellular location inferred from isozyme identity alone. Every native record was inspected; see reports/causal-graph-diagram-review.json for native-object counts, exclusions and identity corrections. Semantic validation and native GraphId locator checks passed; authority snapshots and full corpus gates are coordinated separately.
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