Triglyceride biosynthesis

WikiPathways:WP266

Triglyceride biosynthesis converts sn-glycerol 3-phosphate to 1-acyl-sn-glycerol 3-phosphate, 1,2-diacyl-sn-glycerol 3-phosphate, 1,2-diacyl-sn-glycerol, and triacyl-sn-glycerol in Saccharomyces cerevisiae.

Pathway type

lipid biosynthesis

Organism scope

Saccharomyces cerevisiae NCBITaxon:4932

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Pathway network

25 nodes · 29 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae `NCBITaxon:4932`	taxon
SLC1 `SGD:S000002210`	protein
DGA1 `SGD:S000005771`	protein
1,2-diacyl-sn-glycerol 3-phosphate `CHEBI:29089`	lipid
hydrogenphosphate `CHEBI:43474`	small molecule
SCT1 `SGD:S000000107`	protein
PAH1 `SGD:S000004775`	protein
triacyl-sn-glycerol `CHEBI:64615`	lipid
1-O-acyl-sn-glycero-3-phosphocholine(1+) `CHEBI:17504`	lipid
1,2-diacyl-sn-glycerol `CHEBI:17815`	lipid
acyl-CoA `CHEBI:17984`	small molecule
1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	lipid
water `CHEBI:15377`	small molecule
1,2-diacyl-sn-glycero-3-phosphocholine(1+) `CHEBI:16110`	lipid
sn-glycerol 3-phosphate `CHEBI:57597`	small molecule
GPT2 `SGD:S000001775`	protein
coenzyme A `CHEBI:15346`	small molecule
DPP1 `SGD:S000002692`	protein
LPP1 `SGD:S000002911`	protein
LRO1 `SGD:S000005291`	protein
magnesium(2+) `CHEBI:18420`	cofactor
1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id1cd7ea26`	molecular activity	left to right
1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id3bcd2f4`	molecular activity	left to right
1,2-diacyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id60909eec`	molecular activity	left to right
1-acyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/id85e34cbe`	molecular activity	left to right
sn-glycerol 3-phosphate to 1-acyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/idc8402cc8`	molecular activity	left to right

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
1,2-diacyl-sn-glycerol `CHEBI:17815`	consumes	1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id1cd7ea26`	WikiPathways:WP266 Source assertion: GPML id1cd7ea26 connects normalized CHEBI:17815 as an input of interaction id1cd7ea26. Source location `/Pathway/Interaction[@GraphId='id1cd7ea26']/Graphics; /Pathway/DataNode[@GraphId='dce5f']`
1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id1cd7ea26`	produces	triacyl-sn-glycerol `CHEBI:64615`	WikiPathways:WP266 Source assertion: GPML id1cd7ea26 connects normalized CHEBI:64615 as an output of interaction id1cd7ea26. Source location `/Pathway/Interaction[@GraphId='id1cd7ea26']/Graphics; /Pathway/DataNode[@GraphId='cf64d']`
1,2-diacyl-sn-glycerol `CHEBI:17815`	consumes	1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id3bcd2f4`	WikiPathways:WP266 Source assertion: GPML id3bcd2f4 connects normalized CHEBI:17815 as an input of interaction id3bcd2f4. Source location `/Pathway/Interaction[@GraphId='id3bcd2f4']/Graphics; /Pathway/DataNode[@GraphId='dce5f']`
1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id3bcd2f4`	produces	triacyl-sn-glycerol `CHEBI:64615`	WikiPathways:WP266 Source assertion: GPML id3bcd2f4 connects normalized CHEBI:64615 as an output of interaction id3bcd2f4. Source location `/Pathway/Interaction[@GraphId='id3bcd2f4']/Graphics; /Pathway/DataNode[@GraphId='cf64d']`
1,2-diacyl-sn-glycerol 3-phosphate `CHEBI:29089`	consumes	1,2-diacyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id60909eec`	WikiPathways:WP266 Source assertion: GPML id60909eec connects normalized CHEBI:29089 as an input of interaction id60909eec. Source location `/Pathway/Interaction[@GraphId='id60909eec']/Graphics; /Pathway/DataNode[@GraphId='b2af1']`
1,2-diacyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id60909eec`	produces	1,2-diacyl-sn-glycerol `CHEBI:17815`	WikiPathways:WP266 Source assertion: GPML id60909eec connects normalized CHEBI:17815 as an output of interaction id60909eec. Source location `/Pathway/Interaction[@GraphId='id60909eec']/Graphics; /Pathway/DataNode[@GraphId='dce5f']`
1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	consumes	1-acyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/id85e34cbe`	WikiPathways:WP266 Source assertion: GPML id85e34cbe connects normalized CHEBI:16975 as an input of interaction id85e34cbe. Source location `/Pathway/Interaction[@GraphId='id85e34cbe']/Graphics; /Pathway/DataNode[@GraphId='f64b0']`
1-acyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/id85e34cbe`	produces	1,2-diacyl-sn-glycerol 3-phosphate `CHEBI:29089`	WikiPathways:WP266 Source assertion: GPML id85e34cbe connects normalized CHEBI:29089 as an output of interaction id85e34cbe. Source location `/Pathway/Interaction[@GraphId='id85e34cbe']/Graphics; /Pathway/DataNode[@GraphId='b2af1']`
sn-glycerol 3-phosphate `CHEBI:57597`	consumes	sn-glycerol 3-phosphate to 1-acyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/idc8402cc8`	WikiPathways:WP266 Source assertion: GPML idc8402cc8 connects normalized CHEBI:57597 as an input of interaction idc8402cc8. Source location `/Pathway/Interaction[@GraphId='idc8402cc8']/Graphics; /Pathway/DataNode[@GraphId='fe042']`
sn-glycerol 3-phosphate to 1-acyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/idc8402cc8`	produces	1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	WikiPathways:WP266 Source assertion: GPML idc8402cc8 connects normalized CHEBI:16975 as an output of interaction idc8402cc8. Source location `/Pathway/Interaction[@GraphId='idc8402cc8']/Graphics; /Pathway/DataNode[@GraphId='f64b0']`
SLC1 `SGD:S000002210`	catalyzes	1-acyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/id85e34cbe`	WikiPathways:WP266 Source assertion: GPML id44d2daef connects normalized SGD:S000002210 as a catalyst of interaction id85e34cbe. Source location `/Pathway/Interaction[@GraphId='id44d2daef']/Graphics; /Pathway/DataNode[@GraphId='a87']`
1,2-diacyl-sn-glycero-3-phosphocholine(1+) `CHEBI:16110`	consumes	1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id1cd7ea26`	WikiPathways:WP266 Source assertion: GPML id5db6d8ca connects normalized CHEBI:16110 as an input of interaction id1cd7ea26. Source location `/Pathway/Interaction[@GraphId='id5db6d8ca']/Graphics; /Pathway/DataNode[@GraphId='fc4d6']`
1,2-diacyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id60909eec`	produces	hydrogenphosphate `CHEBI:43474`	WikiPathways:WP266 Source assertion: GPML id8d4f483b connects normalized CHEBI:43474 as an output of interaction id60909eec. Source location `/Pathway/Interaction[@GraphId='id8d4f483b']/Graphics; /Pathway/DataNode[@GraphId='c3644']`
SCT1 `SGD:S000000107`	catalyzes	sn-glycerol 3-phosphate to 1-acyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/idc8402cc8`	WikiPathways:WP266 Source assertion: GPML id910aff3b connects normalized SGD:S000000107 as a catalyst of interaction idc8402cc8. Source location `/Pathway/Interaction[@GraphId='id910aff3b']/Graphics; /Pathway/DataNode[@GraphId='cb880']`
GPT2 `SGD:S000001775`	catalyzes	sn-glycerol 3-phosphate to 1-acyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/idc8402cc8`	WikiPathways:WP266 Source assertion: GPML id910aff3b connects normalized SGD:S000001775 as a catalyst of interaction idc8402cc8. Source location `/Pathway/Interaction[@GraphId='id910aff3b']/Graphics; /Pathway/DataNode[@GraphId='afdf5']`
DGA1 `SGD:S000005771`	catalyzes	1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id3bcd2f4`	WikiPathways:WP266 Source assertion: GPML id9a72ee72 connects normalized SGD:S000005771 as a catalyst of interaction id3bcd2f4. Source location `/Pathway/Interaction[@GraphId='id9a72ee72']/Graphics; /Pathway/DataNode[@GraphId='aef']`
1-acyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/id85e34cbe`	produces	coenzyme A `CHEBI:15346`	WikiPathways:WP266 Source assertion: GPML idb9c5729 connects normalized CHEBI:15346 as an output of interaction id85e34cbe. Source location `/Pathway/Interaction[@GraphId='idb9c5729']/Graphics; /Pathway/DataNode[@GraphId='af189']`
acyl-CoA `CHEBI:17984`	consumes	sn-glycerol 3-phosphate to 1-acyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/idc8402cc8`	WikiPathways:WP266 Source assertion: GPML idc9355581 connects normalized CHEBI:17984 as an input of interaction idc8402cc8. Source location `/Pathway/Interaction[@GraphId='idc9355581']/Graphics; /Pathway/DataNode[@GraphId='df041']`
acyl-CoA `CHEBI:17984`	consumes	1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id3bcd2f4`	WikiPathways:WP266 Source assertion: GPML idd980ea96 connects normalized CHEBI:17984 as an input of interaction id3bcd2f4. Source location `/Pathway/Interaction[@GraphId='idd980ea96']/Graphics; /Pathway/DataNode[@GraphId='c78a3']`
acyl-CoA `CHEBI:17984`	consumes	1-acyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/id85e34cbe`	WikiPathways:WP266 Source assertion: GPML ide84619a9 connects normalized CHEBI:17984 as an input of interaction id85e34cbe. Source location `/Pathway/Interaction[@GraphId='ide84619a9']/Graphics; /Pathway/DataNode[@GraphId='aacf4']`
water `CHEBI:15377`	consumes	1,2-diacyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id60909eec`	WikiPathways:WP266 Source assertion: GPML idecb8710a connects normalized CHEBI:15377 as an input of interaction id60909eec. Source location `/Pathway/Interaction[@GraphId='idecb8710a']/Graphics; /Pathway/DataNode[@GraphId='f8051']`
LRO1 `SGD:S000005291`	catalyzes	1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id1cd7ea26`	WikiPathways:WP266 Source assertion: GPML idf55f4b1c connects normalized SGD:S000005291 as a catalyst of interaction id1cd7ea26. Source location `/Pathway/Interaction[@GraphId='idf55f4b1c']/Graphics; /Pathway/DataNode[@GraphId='c9ac1']`
1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id1cd7ea26`	produces	1-O-acyl-sn-glycero-3-phosphocholine(1+) `CHEBI:17504`	WikiPathways:WP266 Source assertion: GPML idf876cb69 connects normalized CHEBI:17504 as an output of interaction id1cd7ea26. Source location `/Pathway/Interaction[@GraphId='idf876cb69']/Graphics; /Pathway/DataNode[@GraphId='d97ed']`
PAH1 `SGD:S000004775`	catalyzes	1,2-diacyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id60909eec`	WikiPathways:WP266 Source assertion: GPML idf9c930cc connects normalized SGD:S000004775 as a catalyst of interaction id60909eec. Source location `/Pathway/Interaction[@GraphId='idf9c930cc']/Graphics; /Pathway/DataNode[@GraphId='cf077']`
DPP1 `SGD:S000002692`	catalyzes	1,2-diacyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id60909eec`	WikiPathways:WP266 Source assertion: GPML idf9c930cc connects normalized SGD:S000002692 as a catalyst of interaction id60909eec. Source location `/Pathway/Interaction[@GraphId='idf9c930cc']/Graphics; /Pathway/DataNode[@GraphId='f5e7a']`
LPP1 `SGD:S000002911`	catalyzes	1,2-diacyl-sn-glycerol 3-phosphate to 1,2-diacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id60909eec`	WikiPathways:WP266 Source assertion: GPML idf9c930cc connects normalized SGD:S000002911 as a catalyst of interaction id60909eec. Source location `/Pathway/Interaction[@GraphId='idf9c930cc']/Graphics; /Pathway/DataNode[@GraphId='b48ef']`
sn-glycerol 3-phosphate to 1-acyl-sn-glycerol 3-phosphate GPML interaction `WikiPathways:WP266/idc8402cc8`	produces	coenzyme A `CHEBI:15346`	WikiPathways:WP266 Source assertion: GPML e60b8 connects normalized CHEBI:15346 as an output of interaction idc8402cc8. Source location `/Pathway/Interaction[@GraphId='e60b8']/Graphics; /Pathway/DataNode[@GraphId='b1ab6']`
1,2-diacyl-sn-glycerol to triacyl-sn-glycerol GPML interaction `WikiPathways:WP266/id3bcd2f4`	produces	coenzyme A `CHEBI:15346`	WikiPathways:WP266 Source assertion: GPML e6580 connects normalized CHEBI:15346 as an output of interaction id3bcd2f4. Source location `/Pathway/Interaction[@GraphId='e6580']/Graphics; /Pathway/DataNode[@GraphId='aa880']`
PAH1 `SGD:S000004775`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:16467296)	magnesium(2+) `CHEBI:18420`	UniProtKB:P32567 Source assertion: UniProtKB:P32567 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/949/comments/0/cofactors/0` UniProtKB:P32567 Source assertion: UniProtKB:P32567 has the exact SGD cross-reference SGD:S000004775. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/949/uniProtKBCrossReferences/0`

References

WikiPathways:WP266 WP266 native structured pathway source (source version: WP266_r139569)
UniProtKB:P32567 UniProtKB P32567 cofactor annotations (source version: 2026_03)

Curation history

2026-10-05T19:29:52Z Codex; adversarial-scientific-correction; Corrected sn-glycerol 3-phosphate(2-) CHEBI:57597 to small_molecule using ChEBI release 255 structural classification; biochemical edges and original source assertions are unchanged.
2026-10-05T06:17:10Z Codex
- EDIT — Review complete causal graph against native sources and correct unsupported or omitted relationships
  Restored acyl-CoA/CoA, water/phosphate, PC/lysoPC and enzymes; LRO1 and DGA1 remain distinct routes to TAG. Every native record was inspected; see reports/causal-graph-diagram-review.json for native-object counts, exclusions and identity corrections. Semantic validation and native GraphId locator checks passed; authority snapshots and full corpus gates are coordinated separately.
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2026-10-05T19:31:11Z Codex
- EDIT — Corrected sn-glycerol 3-phosphate(2-) CHEBI:57597 to small_molecule using ChEBI release 255 structural classification; biochemical edges and original source assertions are unchanged.
  Corrected sn-glycerol 3-phosphate(2-) CHEBI:57597 to small_molecule using ChEBI release 255 structural classification; biochemical edges and original source assertions are unchanged. Original source assertions and review scope are preserved in reports/causal_graph_review/adversarial-scientific-corrections.json. Validated the changed record against the closed schema and added focused regression checks; repository-wide validation and generated artifacts are handled in the integration step.
Read this curation session