L-asparagine biosynthesis I

gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1

L-asparagine biosynthesis I aminates oxaloacetate to L-aspartate and then amidates L-aspartate to L-asparagine through paired AAT1/AAT2 and ASN1/ASN2 activities in Saccharomyces cerevisiae S288C.

Pathway type

amino acid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

25 nodes · 46 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
L-glutamine zwitterion `CHEBI:58359`	small molecule
diphosphoric acid `CHEBI:29888`	small molecule
hydron `CHEBI:15378`	small molecule
L-asparagine zwitterion `CHEBI:58048`	small molecule
2-oxoglutarate(2-) `CHEBI:16810`	small molecule
oxaloacetate(2-) `CHEBI:16452`	small molecule
L-glutamate(1-) `CHEBI:29985`	small molecule
L-asparagine biosynthetic process `GO:0070981`	biological process
ATP(4-) `CHEBI:30616`	small molecule
ASN1 Scer `SGD:S000006349`	protein
cytosol `GO:0005829`	cellular component
AAT1 Scer `SGD:S000001589`	protein
water `CHEBI:15377`	small molecule
L-aspartate(1-) `CHEBI:29991`	small molecule
adenosine 5'-monophosphate(2-) `CHEBI:456215`	small molecule
AAT2 Scer `SGD:S000004017`	protein
ASN2 Scer `SGD:S000003356`	protein
mitochondrial matrix `GO:0005759`	cellular component
cytoplasm `GO:0005737`	cellular component
peroxisome `GO:0005777`	cellular component
pyridoxal 5'-phosphate(2-) `CHEBI:597326`	cofactor
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	molecular activity
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	molecular activity
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	molecular activity
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	has_input	L-glutamine zwitterion `CHEBI:58359`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002233 gomodel:CHEBI_58359_ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/0; endpoint types: /individuals/0/type; /individuals/13/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	provides_input_for	asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553 RO:0002413 gomodel:ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/1; endpoint types: /individuals/37/type; /individuals/0/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002234 gomodel:CHEBI_29888_ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/2; endpoint types: /individuals/0/type; /individuals/6/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002234 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003574. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/3; endpoint types: /individuals/51/type; /individuals/57/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	has_output	L-asparagine zwitterion `CHEBI:58048`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002234 gomodel:CHEBI_58048_ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/4; endpoint types: /individuals/0/type; /individuals/12/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	has_output	2-oxoglutarate(2-) `CHEBI:16810`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002234 gomodel:CHEBI_16810_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/5; endpoint types: /individuals/1/type; /individuals/5/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	provides_input_for	asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002413 gomodel:ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/6; endpoint types: /individuals/1/type; /individuals/0/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	has_input	oxaloacetate(2-) `CHEBI:16452`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002233 gomodel:CHEBI_16452_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/7; endpoint types: /individuals/1/type; /individuals/4/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	has_output	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002234 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003575. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/8; endpoint types: /individuals/51/type; /individuals/58/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	provides_input_for	asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002413 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/9; endpoint types: /individuals/1/type; /individuals/51/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	part_of	L-asparagine biosynthetic process `GO:0070981`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN BFO:0000050 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/YeastPathways_ASPARAGINE-BIOSYNTHESIS-1. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/10; endpoint types: /individuals/0/type; /individuals/35/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002234 gomodel:CHEBI_15378_ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/11; endpoint types: /individuals/0/type; /individuals/3/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002233 gomodel:CHEBI_30616_ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/12; endpoint types: /individuals/0/type; /individuals/10/type`
ASN1 Scer `SGD:S000006349`	enables	asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002333 gomodel:YPR145W-MONOMER_ASNSYNB-RXN_controller. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/13; endpoint types: /individuals/0/type; /individuals/15/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	part_of	L-asparagine biosynthetic process `GO:0070981`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 BFO:0000050 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/YeastPathways_ASPARAGINE-BIOSYNTHESIS-1. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/15; endpoint types: /individuals/51/type; /individuals/35/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	has_input	oxaloacetate(2-) `CHEBI:16452`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553 RO:0002233 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003555. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/16; endpoint types: /individuals/37/type; /individuals/39/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	has_input	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553 RO:0002233 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003554. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/17; endpoint types: /individuals/37/type; /individuals/38/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	has_output	2-oxoglutarate(2-) `CHEBI:16810`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553 RO:0002234 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003556. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/18; endpoint types: /individuals/37/type; /individuals/40/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002234 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003572. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/19; endpoint types: /individuals/51/type; /individuals/55/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	has_output	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002234 gomodel:CHEBI_29985_ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/20; endpoint types: /individuals/0/type; /individuals/7/type`
AAT1 Scer `SGD:S000001589`	enables	L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002333 gomodel:YKL106W-MONOMER_ASPAMINOTRANS-RXN_controller. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/21; endpoint types: /individuals/1/type; /individuals/14/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	has_input	L-glutamine zwitterion `CHEBI:58359`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002233 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003569. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/22; endpoint types: /individuals/51/type; /individuals/52/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	part_of	L-asparagine biosynthetic process `GO:0070981`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553 BFO:0000050 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/YeastPathways_ASPARAGINE-BIOSYNTHESIS-1. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/23; endpoint types: /individuals/37/type; /individuals/35/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002233 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003570. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/24; endpoint types: /individuals/51/type; /individuals/53/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 BFO:0000066 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003579. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/25; endpoint types: /individuals/51/type; /individuals/61/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002233 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003571. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/26; endpoint types: /individuals/51/type; /individuals/54/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	part_of	L-asparagine biosynthetic process `GO:0070981`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN BFO:0000050 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/YeastPathways_ASPARAGINE-BIOSYNTHESIS-1. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/27; endpoint types: /individuals/1/type; /individuals/35/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	has_input	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002233 gomodel:CHEBI_29985_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/28; endpoint types: /individuals/1/type; /individuals/8/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	has_output	L-aspartate(1-) `CHEBI:29991`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553 RO:0002234 gomodel:CHEBI_29991_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/29; endpoint types: /individuals/37/type; /individuals/9/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	has_output	L-asparagine zwitterion `CHEBI:58048`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002234 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003573. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/30; endpoint types: /individuals/51/type; /individuals/56/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	has_input	L-aspartate(1-) `CHEBI:29991`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002233 gomodel:CHEBI_29991_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/31; endpoint types: /individuals/51/type; /individuals/9/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002233 gomodel:CHEBI_15377_ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/32; endpoint types: /individuals/0/type; /individuals/2/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	has_output	adenosine 5'-monophosphate(2-) `CHEBI:456215`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002234 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003576. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/33; endpoint types: /individuals/51/type; /individuals/59/type`
AAT2 Scer `SGD:S000004017`	enables	L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553 RO:0002333 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003557. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/34; endpoint types: /individuals/37/type; /individuals/41/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN BFO:0000066 gomodel:reaction_ASNSYNB-RXN_location_lociGO_0005829. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/35; endpoint types: /individuals/0/type; /individuals/33/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553 BFO:0000066 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003559. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/36; endpoint types: /individuals/37/type; /individuals/42/type`
ASN2 Scer `SGD:S000003356`	enables	asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568 RO:0002333 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003578. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/37; endpoint types: /individuals/51/type; /individuals/60/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	has_input	L-aspartate(1-) `CHEBI:29991`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002233 gomodel:CHEBI_29991_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/38; endpoint types: /individuals/0/type; /individuals/9/type`
asparagine synthase (glutamine-hydrolyzing) activity `gomodel:ASNSYNB-RXN`	has_output	adenosine 5'-monophosphate(2-) `CHEBI:456215`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASNSYNB-RXN RO:0002234 gomodel:CHEBI_456215_ASNSYNB-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/39; endpoint types: /individuals/0/type; /individuals/11/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553`	provides_input_for	asparagine synthase (glutamine-hydrolyzing) activity `gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003553 RO:0002413 gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1/6a4c244800003568. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/40; endpoint types: /individuals/37/type; /individuals/51/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	has_output	L-aspartate(1-) `CHEBI:29991`	gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002234 gomodel:CHEBI_29991_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json#/facts/41; endpoint types: /individuals/1/type; /individuals/9/type`
AAT1 Scer `SGD:S000001589`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:14576278)	mitochondrial matrix `GO:0005759`	UniProtKB:Q01802 Source assertion: UniProtKB:Q01802 annotates location Mitochondrion matrix (SL-0170). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4042/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q01802 Source assertion: UniProtKB:Q01802 has the exact SGD cross-reference SGD:S000001589. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4042/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
AAT2 Scer `SGD:S000004017`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:9288922) Source qualification: Targeted to peroxisomes in cells grown in oleate	cytoplasm `GO:0005737`	UniProtKB:P23542 Source assertion: UniProtKB:P23542 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/619/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P23542 Source assertion: UniProtKB:P23542 has the exact SGD cross-reference SGD:S000004017. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/619/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
AAT2 Scer `SGD:S000004017`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:9288922) Source qualification: Targeted to peroxisomes in cells grown in oleate	peroxisome `GO:0005777`	UniProtKB:P23542 Source assertion: UniProtKB:P23542 annotates location Peroxisome (SL-0204). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/619/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P23542 Source assertion: UniProtKB:P23542 has the exact SGD cross-reference SGD:S000004017. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/619/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
AAT1 Scer `SGD:S000001589`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:Q01802 Source assertion: UniProtKB:Q01802 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4042/comments/0/cofactors/0` UniProtKB:Q01802 Source assertion: UniProtKB:Q01802 has the exact SGD cross-reference SGD:S000001589. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4042/uniProtKBCrossReferences/0`
AAT2 Scer `SGD:S000004017`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:6811576); ECO:0000269 (PubMed:9655342)	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P23542 Source assertion: UniProtKB:P23542 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/619/comments/0/cofactors/0` UniProtKB:P23542 Source assertion: UniProtKB:P23542 has the exact SGD cross-reference SGD:S000004017. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/619/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 GO-CAM L-asparagine biosynthesis I model YeastPathways_ASPARAGINE-BIOSYNTHESIS-1 (source version: Pinned Noctua JSON archive; model date 2026-07-15; member ./YeastPathways_ASPARAGINE-BIOSYNTHESIS-1.json)
UniProtKB:Q01802 Reviewed UniProtKB Q01802 location annotations (source version: UniProt release 2026_03)
UniProtKB:P23542 Reviewed UniProtKB P23542 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 4 activity location assignments and 4 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 3 independently supported location edges and quarantined 1 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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