aspartate biosynthesis

gomodel:YeastPathways_ASPBIO-PWY

Aspartate biosynthesis in Saccharomyces cerevisiae S288C uses PYC1 and PYC2 pyruvate carboxylase activities to carboxylate pyruvate to oxaloacetate and AAT1 and AAT2 L-aspartate:2-oxoglutarate transaminase activities to transaminate oxaloacetate and L-glutamate to L-aspartate and 2-oxoglutarate.

Pathway type

amino acid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

Read the source YAML

Pathway network

26 nodes · 44 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

Show at full size (scroll to explore)

Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
L-aspartate(1-) `CHEBI:29991`	small molecule
pyruvate `CHEBI:15361`	small molecule
oxaloacetate(2-) `CHEBI:16452`	small molecule
ADP(3-) `CHEBI:456216`	small molecule
hydron `CHEBI:15378`	small molecule
hydrogenphosphate `CHEBI:43474`	small molecule
L-aspartate biosynthetic process `GO:0006532`	biological process
L-glutamate(1-) `CHEBI:29985`	small molecule
cytosol `GO:0005829`	cellular component
AAT2 Scer `SGD:S000004017`	protein
hydrogencarbonate `CHEBI:17544`	small molecule
AAT1 Scer `SGD:S000001589`	protein
ATP(4-) `CHEBI:30616`	small molecule
2-oxoglutarate(2-) `CHEBI:16810`	small molecule
PYC1 Scer `SGD:S000003030`	protein
PYC2 Scer `SGD:S000000422`	protein
cytoplasm `GO:0005737`	cellular component
peroxisome `GO:0005777`	cellular component
mitochondrial matrix `GO:0005759`	cellular component
pyridoxal 5'-phosphate(2-) `CHEBI:597326`	cofactor
biotinate `CHEBI:57586`	cofactor
zinc(2+) `CHEBI:29105`	cofactor
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	molecular activity
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	molecular activity
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	molecular activity
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	has_output	L-aspartate(1-) `CHEBI:29991`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002234 gomodel:CHEBI_29991_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/0; endpoint types: /individuals/0/type; /individuals/7/type`
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	has_input	pyruvate `CHEBI:15361`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 RO:0002233 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002250. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/1; endpoint types: /individuals/32/type; /individuals/34/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268`	has_input	oxaloacetate(2-) `CHEBI:16452`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268 RO:0002233 gomodel:CHEBI_16452_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/2; endpoint types: /individuals/51/type; /individuals/3/type`
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	has_output	ADP(3-) `CHEBI:456216`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN RO:0002234 gomodel:CHEBI_456216_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/3; endpoint types: /individuals/11/type; /individuals/10/type`
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN RO:0002234 gomodel:CHEBI_15378_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/4; endpoint types: /individuals/11/type; /individuals/2/type`
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	has_output	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 RO:0002234 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002254. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/5; endpoint types: /individuals/32/type; /individuals/38/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268`	part_of	L-aspartate biosynthetic process `GO:0006532`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268 BFO:0000050 gomodel:YeastPathways_ASPBIO-PWY/YeastPathways_ASPBIO-PWY. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/6; endpoint types: /individuals/51/type; /individuals/31/type`
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 RO:0002234 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002253. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/7; endpoint types: /individuals/32/type; /individuals/37/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	has_input	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002233 gomodel:CHEBI_29985_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/8; endpoint types: /individuals/0/type; /individuals/6/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268 BFO:0000066 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002274. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/9; endpoint types: /individuals/51/type; /individuals/56/type`
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	part_of	L-aspartate biosynthetic process `GO:0006532`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN BFO:0000050 gomodel:YeastPathways_ASPBIO-PWY/YeastPathways_ASPBIO-PWY. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/10; endpoint types: /individuals/11/type; /individuals/31/type`
AAT2 Scer `SGD:S000004017`	enables	L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268 RO:0002333 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002272. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/12; endpoint types: /individuals/51/type; /individuals/55/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268`	has_output	L-aspartate(1-) `CHEBI:29991`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268 RO:0002234 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002270. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/13; endpoint types: /individuals/51/type; /individuals/53/type`
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	has_output	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN RO:0002234 gomodel:CHEBI_43474_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/14; endpoint types: /individuals/11/type; /individuals/9/type`
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	has_input	hydrogencarbonate `CHEBI:17544`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 RO:0002233 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002249. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/15; endpoint types: /individuals/32/type; /individuals/33/type`
AAT1 Scer `SGD:S000001589`	enables	L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002333 gomodel:YKL106W-MONOMER_ASPAMINOTRANS-RXN_controller. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/16; endpoint types: /individuals/0/type; /individuals/13/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	part_of	L-aspartate biosynthetic process `GO:0006532`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN BFO:0000050 gomodel:YeastPathways_ASPBIO-PWY/YeastPathways_ASPBIO-PWY. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/17; endpoint types: /individuals/0/type; /individuals/31/type`
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 RO:0002233 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002251. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/18; endpoint types: /individuals/32/type; /individuals/35/type`
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 BFO:0000066 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002257. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/19; endpoint types: /individuals/32/type; /individuals/40/type`
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	has_input	hydrogencarbonate `CHEBI:17544`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN RO:0002233 gomodel:CHEBI_17544_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/20; endpoint types: /individuals/11/type; /individuals/5/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	has_output	2-oxoglutarate(2-) `CHEBI:16810`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002234 gomodel:CHEBI_16810_ASPAMINOTRANS-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/21; endpoint types: /individuals/0/type; /individuals/4/type`
PYC1 Scer `SGD:S000003030`	enables	pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN RO:0002333 gomodel:YGL062W-MONOMER_PYRUVATE-CARBOXYLASE-RXN_controller. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/22; endpoint types: /individuals/11/type; /individuals/12/type`
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	has_output	ADP(3-) `CHEBI:456216`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 RO:0002234 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002252. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/23; endpoint types: /individuals/32/type; /individuals/36/type`
PYC2 Scer `SGD:S000000422`	enables	pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 RO:0002333 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002255. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/24; endpoint types: /individuals/32/type; /individuals/39/type`
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN BFO:0000066 gomodel:reaction_PYRUVATE-CARBOXYLASE-RXN_location_lociGO_0005829. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/25; endpoint types: /individuals/11/type; /individuals/30/type`
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	has_input	pyruvate `CHEBI:15361`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN RO:0002233 gomodel:CHEBI_15361_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/26; endpoint types: /individuals/11/type; /individuals/1/type`
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	part_of	L-aspartate biosynthetic process `GO:0006532`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 BFO:0000050 gomodel:YeastPathways_ASPBIO-PWY/YeastPathways_ASPBIO-PWY. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/27; endpoint types: /individuals/32/type; /individuals/31/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:ASPAMINOTRANS-RXN`	has_input	oxaloacetate(2-) `CHEBI:16452`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:ASPAMINOTRANS-RXN RO:0002233 gomodel:CHEBI_16452_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/28; endpoint types: /individuals/0/type; /individuals/3/type`
pyruvate carboxylase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248`	has_output	oxaloacetate(2-) `CHEBI:16452`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002248 RO:0002234 gomodel:CHEBI_16452_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/29; endpoint types: /individuals/32/type; /individuals/3/type`
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	has_output	oxaloacetate(2-) `CHEBI:16452`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN RO:0002234 gomodel:CHEBI_16452_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/30; endpoint types: /individuals/11/type; /individuals/3/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268`	has_output	2-oxoglutarate(2-) `CHEBI:16810`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268 RO:0002234 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002271. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/31; endpoint types: /individuals/51/type; /individuals/54/type`
L-aspartate:2-oxoglutarate transaminase activity `gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268`	has_input	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002268 RO:0002233 gomodel:YeastPathways_ASPBIO-PWY/6a4c244800002269. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/32; endpoint types: /individuals/51/type; /individuals/52/type`
pyruvate carboxylase activity `gomodel:PYRUVATE-CARBOXYLASE-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_ASPBIO-PWY Source assertion: The model asserts gomodel:PYRUVATE-CARBOXYLASE-RXN RO:0002233 gomodel:CHEBI_30616_PYRUVATE-CARBOXYLASE-RXN. Source location `./YeastPathways_ASPBIO-PWY.json#/facts/33; endpoint types: /individuals/11/type; /individuals/8/type`
AAT2 Scer `SGD:S000004017`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:9288922) Source qualification: Targeted to peroxisomes in cells grown in oleate	cytoplasm `GO:0005737`	UniProtKB:P23542 Source assertion: UniProtKB:P23542 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/619/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P23542 Source assertion: UniProtKB:P23542 has the exact SGD cross-reference SGD:S000004017. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/619/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
AAT2 Scer `SGD:S000004017`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:9288922) Source qualification: Targeted to peroxisomes in cells grown in oleate	peroxisome `GO:0005777`	UniProtKB:P23542 Source assertion: UniProtKB:P23542 annotates location Peroxisome (SL-0204). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/619/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P23542 Source assertion: UniProtKB:P23542 has the exact SGD cross-reference SGD:S000004017. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/619/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
AAT1 Scer `SGD:S000001589`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:14576278)	mitochondrial matrix `GO:0005759`	UniProtKB:Q01802 Source assertion: UniProtKB:Q01802 annotates location Mitochondrion matrix (SL-0170). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4042/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q01802 Source assertion: UniProtKB:Q01802 has the exact SGD cross-reference SGD:S000001589. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4042/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
PYC1 Scer `SGD:S000003030`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	cytoplasm `GO:0005737`	UniProtKB:P11154 Source assertion: UniProtKB:P11154 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/322/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P11154 Source assertion: UniProtKB:P11154 has the exact SGD cross-reference SGD:S000003030. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/322/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
PYC2 Scer `SGD:S000000422`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	cytoplasm `GO:0005737`	UniProtKB:P32327 Source assertion: UniProtKB:P32327 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/866/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32327 Source assertion: UniProtKB:P32327 has the exact SGD cross-reference SGD:S000000422. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/866/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
AAT2 Scer `SGD:S000004017`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:6811576); ECO:0000269 (PubMed:9655342)	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P23542 Source assertion: UniProtKB:P23542 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/619/comments/0/cofactors/0` UniProtKB:P23542 Source assertion: UniProtKB:P23542 has the exact SGD cross-reference SGD:S000004017. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/619/uniProtKBCrossReferences/0`
AAT1 Scer `SGD:S000001589`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:Q01802 Source assertion: UniProtKB:Q01802 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4042/comments/0/cofactors/0` UniProtKB:Q01802 Source assertion: UniProtKB:Q01802 has the exact SGD cross-reference SGD:S000001589. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4042/uniProtKBCrossReferences/0`
PYC1 Scer `SGD:S000003030`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	biotinate `CHEBI:57586`	UniProtKB:P11154 Source assertion: UniProtKB:P11154 annotates CHEBI:57586 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/322/comments/0/cofactors/0` UniProtKB:P11154 Source assertion: UniProtKB:P11154 has the exact SGD cross-reference SGD:S000003030. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/322/uniProtKBCrossReferences/0`
PYC1 Scer `SGD:S000003030`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	zinc(2+) `CHEBI:29105`	UniProtKB:P11154 Source assertion: UniProtKB:P11154 annotates CHEBI:29105 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/322/comments/1/cofactors/0` UniProtKB:P11154 Source assertion: UniProtKB:P11154 has the exact SGD cross-reference SGD:S000003030. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/322/uniProtKBCrossReferences/0`
PYC2 Scer `SGD:S000000422`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	biotinate `CHEBI:57586`	UniProtKB:P32327 Source assertion: UniProtKB:P32327 annotates CHEBI:57586 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/866/comments/0/cofactors/0` UniProtKB:P32327 Source assertion: UniProtKB:P32327 has the exact SGD cross-reference SGD:S000000422. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/866/uniProtKBCrossReferences/0`
PYC2 Scer `SGD:S000000422`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	zinc(2+) `CHEBI:29105`	UniProtKB:P32327 Source assertion: UniProtKB:P32327 annotates CHEBI:29105 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/866/comments/1/cofactors/0` UniProtKB:P32327 Source assertion: UniProtKB:P32327 has the exact SGD cross-reference SGD:S000000422. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/866/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_ASPBIO-PWY GO-CAM aspartate biosynthesis model YeastPathways_ASPBIO-PWY (source version: Pinned Noctua JSON archive; model date 2026-07-14; member ./YeastPathways_ASPBIO-PWY.json)
UniProtKB:P23542 Reviewed UniProtKB P23542 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q01802 Reviewed UniProtKB Q01802 location annotations (source version: UniProt release 2026_03)
UniProtKB:P11154 Reviewed UniProtKB P11154 location annotations (source version: UniProt release 2026_03)
UniProtKB:P32327 Reviewed UniProtKB P32327 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 4 activity location assignments and 4 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 5 independently supported location edges and quarantined 1 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
Read this curation session