citrulline biosynthesis

gomodel:YeastPathways_CITRUL-BIO2-PWY

Citrulline biosynthesis in Saccharomyces cerevisiae S288C uses CPX-579 carbamoyl-phosphate synthase to form carbamoyl phosphate from L-glutamine, hydrogencarbonate, water, and ATP, then ARG3 ornithine carbamoyltransferase transfers the carbamoyl group to L-ornithine to form L-citrulline in the SGD GO-CAM citrulline biosynthesis pathway.

Pathway type

amino acid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

22 nodes · 28 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
L-citrulline zwitterion `CHEBI:57743`	small molecule
hydrogenphosphate `CHEBI:43474`	small molecule
hydrogencarbonate `CHEBI:17544`	small molecule
ADP(3-) `CHEBI:456216`	small molecule
water `CHEBI:15377`	small molecule
hydron `CHEBI:15378`	small molecule
cytosol `GO:0005829`	cellular component
L-citrulline biosynthetic process `GO:0019240`	biological process
carbamoyl phosphate(2-) `CHEBI:58228`	small molecule
CPX-579 Scer `SGD:S000217863`	complex
ARG3 Scer `SGD:S000003624`	protein
L-glutamate(1-) `CHEBI:29985`	small molecule
L-glutamine zwitterion `CHEBI:58359`	small molecule
L-ornithinium(1+) `CHEBI:46911`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
cytoplasm `GO:0005737`	cellular component
CPA2 Scer `SGD:S000003870`	protein
CPA1 Scer `SGD:S000005829`	protein
magnesium(2+) `CHEBI:18420`	cofactor
manganese(2+) `CHEBI:29035`	cofactor
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	molecular activity
L-ornithine + carbamoyl phosphate -> L-citrulline + phosphate + H⁺ `gomodel:ORNCARBAMTRANSFER-RXN`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
L-ornithine + carbamoyl phosphate -> L-citrulline + phosphate + H⁺ `gomodel:ORNCARBAMTRANSFER-RXN`	has_output	L-citrulline zwitterion `CHEBI:57743`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:ORNCARBAMTRANSFER-RXN RO:0002234 gomodel:CHEBI_57743_ORNCARBAMTRANSFER-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/0; endpoint types: /individuals/14/type; /individuals/11/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	has_output	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002234 gomodel:CHEBI_43474_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/1; endpoint types: /individuals/0/type; /individuals/7/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	has_input	hydrogencarbonate `CHEBI:17544`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002233 gomodel:CHEBI_17544_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/2; endpoint types: /individuals/0/type; /individuals/4/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	has_output	ADP(3-) `CHEBI:456216`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002234 gomodel:CHEBI_456216_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/3; endpoint types: /individuals/0/type; /individuals/9/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002233 gomodel:CHEBI_15377_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/4; endpoint types: /individuals/0/type; /individuals/1/type`
L-ornithine + carbamoyl phosphate -> L-citrulline + phosphate + H⁺ `gomodel:ORNCARBAMTRANSFER-RXN`	has_output	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:ORNCARBAMTRANSFER-RXN RO:0002234 gomodel:CHEBI_43474_ORNCARBAMTRANSFER-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/5; endpoint types: /individuals/14/type; /individuals/8/type`
L-ornithine + carbamoyl phosphate -> L-citrulline + phosphate + H⁺ `gomodel:ORNCARBAMTRANSFER-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:ORNCARBAMTRANSFER-RXN RO:0002234 gomodel:CHEBI_15378_ORNCARBAMTRANSFER-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/6; endpoint types: /individuals/14/type; /individuals/3/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002234 gomodel:CHEBI_15378_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/7; endpoint types: /individuals/0/type; /individuals/2/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN BFO:0000066 gomodel:reaction_CARBPSYN-RXN_location_lociGO_0005829. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/8; endpoint types: /individuals/0/type; /individuals/33/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	part_of	L-citrulline biosynthetic process `GO:0019240`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN BFO:0000050 gomodel:YeastPathways_CITRUL-BIO2-PWY/YeastPathways_CITRUL-BIO2-PWY. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/9; endpoint types: /individuals/0/type; /individuals/35/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	has_output	carbamoyl phosphate(2-) `CHEBI:58228`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002234 gomodel:CHEBI_58228_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/10; endpoint types: /individuals/0/type; /individuals/12/type`
CPX-579 Scer `SGD:S000217863`	enables	2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002333 gomodel:YeastPathways_CITRUL-BIO2-PWY/6690711d00000301. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/11; endpoint types: /individuals/0/type; /individuals/36/type`
ARG3 Scer `SGD:S000003624`	enables	L-ornithine + carbamoyl phosphate -> L-citrulline + phosphate + H⁺ `gomodel:ORNCARBAMTRANSFER-RXN`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:ORNCARBAMTRANSFER-RXN RO:0002333 gomodel:YJL088W-MONOMER_ORNCARBAMTRANSFER-RXN_controller. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/12; endpoint types: /individuals/14/type; /individuals/15/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	has_output	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002234 gomodel:CHEBI_29985_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/13; endpoint types: /individuals/0/type; /individuals/5/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	has_input	L-glutamine zwitterion `CHEBI:58359`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002233 gomodel:CHEBI_58359_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/14; endpoint types: /individuals/0/type; /individuals/13/type`
L-ornithine + carbamoyl phosphate -> L-citrulline + phosphate + H⁺ `gomodel:ORNCARBAMTRANSFER-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:ORNCARBAMTRANSFER-RXN BFO:0000066 gomodel:reaction_ORNCARBAMTRANSFER-RXN_location_lociGO_0005829. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/15; endpoint types: /individuals/14/type; /individuals/34/type`
L-ornithine + carbamoyl phosphate -> L-citrulline + phosphate + H⁺ `gomodel:ORNCARBAMTRANSFER-RXN`	has_input	L-ornithinium(1+) `CHEBI:46911`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:ORNCARBAMTRANSFER-RXN RO:0002233 gomodel:CHEBI_46911_ORNCARBAMTRANSFER-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/16; endpoint types: /individuals/14/type; /individuals/10/type`
2 ATP + L-glutamine + hydrogencarbonate + H₂O -> L-glutamate + carbamoyl phosphate + 2 ADP + phosphate + 2 H⁺ `gomodel:CARBPSYN-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:CARBPSYN-RXN RO:0002233 gomodel:CHEBI_30616_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/17; endpoint types: /individuals/0/type; /individuals/6/type`
L-ornithine + carbamoyl phosphate -> L-citrulline + phosphate + H⁺ `gomodel:ORNCARBAMTRANSFER-RXN`	has_input	carbamoyl phosphate(2-) `CHEBI:58228`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:ORNCARBAMTRANSFER-RXN RO:0002233 gomodel:CHEBI_58228_CARBPSYN-RXN. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/18; endpoint types: /individuals/14/type; /individuals/12/type`
L-ornithine + carbamoyl phosphate -> L-citrulline + phosphate + H⁺ `gomodel:ORNCARBAMTRANSFER-RXN`	part_of	L-citrulline biosynthetic process `GO:0019240`	gomodel:YeastPathways_CITRUL-BIO2-PWY Source assertion: The model asserts gomodel:ORNCARBAMTRANSFER-RXN BFO:0000050 gomodel:YeastPathways_CITRUL-BIO2-PWY/YeastPathways_CITRUL-BIO2-PWY. Source location `./YeastPathways_CITRUL-BIO2-PWY.json#/facts/19; endpoint types: /individuals/14/type; /individuals/35/type`
CPX-579 Scer `SGD:S000217863`	has_part ECO:0000353: physical interaction evidence; source stoichiometry minValue: 1, maxValue: 1	CPA1 Scer `SGD:S000005829`	SGD:S000217863 Source assertion: Complex Portal CPX-579 lists P07258 as a protein component; complex evidence is ECO:0000353: physical interaction evidence. Source location `Complex Portal JSON #/participants/0; /evidenceType` gomodel:YeastPathways_ARGSYNBSUB-PWY Source assertion: The model asserts gomodel:CPLX3O-887_CARBPSYN-RXN_controller BFO:0000051 gomodel:SGD_S000005829_CPLX3O-887_component. Source location `./YeastPathways_ARGSYNBSUB-PWY.json#/facts/78; endpoint types: /individuals/44/type; /individuals/50/type`
CPX-579 Scer `SGD:S000217863`	has_part ECO:0000353: physical interaction evidence; source stoichiometry minValue: 1, maxValue: 1	CPA2 Scer `SGD:S000003870`	SGD:S000217863 Source assertion: Complex Portal CPX-579 lists P03965 as a protein component; complex evidence is ECO:0000353: physical interaction evidence. Source location `Complex Portal JSON #/participants/1; /evidenceType` gomodel:YeastPathways_ARGSYNBSUB-PWY Source assertion: The model asserts gomodel:CPLX3O-887_CARBPSYN-RXN_controller BFO:0000051 gomodel:SGD_S000003870_CPLX3O-887_component. Source location `./YeastPathways_ARGSYNBSUB-PWY.json#/facts/9; endpoint types: /individuals/44/type; /individuals/49/type`
CPX-579 Scer `SGD:S000217863`	located_in Complex location from the independent composition source; original GO-CAM activity locations are retained with their own provenance.	cytoplasm `GO:0005737`	SGD:S000217863 Source assertion: Complex Portal CPX-579 records cellular location GO:0005737 (cytoplasm); its evidence classification is ECO:0000353: physical interaction evidence. Source location `Complex Portal JSON #/crossReferences/9; /evidenceType`
ARG3 Scer `SGD:S000003624`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	cytoplasm `GO:0005737`	UniProtKB:P05150 Source assertion: UniProtKB:P05150 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/100/comments/0/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P05150 Source assertion: UniProtKB:P05150 has the exact SGD cross-reference SGD:S000003624. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/100/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
CPA2 Scer `SGD:S000003870`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:200419); ECO:0000269 (PubMed:205532)	cytoplasm `GO:0005737`	UniProtKB:P03965 Source assertion: UniProtKB:P03965 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/75/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P03965 Source assertion: UniProtKB:P03965 has the exact SGD cross-reference SGD:S000003870. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/75/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
CPA1 Scer `SGD:S000005829`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:200419); ECO:0000269 (PubMed:205532)	cytoplasm `GO:0005737`	UniProtKB:P07258 Source assertion: UniProtKB:P07258 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/169/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P07258 Source assertion: UniProtKB:P07258 has the exact SGD cross-reference SGD:S000005829. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/169/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
CPA2 Scer `SGD:S000003870`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000255 (PROSITE-ProRule:PRU00409) Binds 4 Mg(2+) or Mn(2+) ions per subunit. UniProt note evidence: ECO:0000255 (PROSITE-ProRule:PRU00409)	magnesium(2+) `CHEBI:18420`	UniProtKB:P03965 Source assertion: UniProtKB:P03965 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/75/comments/0/cofactors/0; /results/75/comments/0/note` UniProtKB:P03965 Source assertion: UniProtKB:P03965 has the exact SGD cross-reference SGD:S000003870. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/75/uniProtKBCrossReferences/0`
CPA2 Scer `SGD:S000003870`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000255 (PROSITE-ProRule:PRU00409) Binds 4 Mg(2+) or Mn(2+) ions per subunit. UniProt note evidence: ECO:0000255 (PROSITE-ProRule:PRU00409)	manganese(2+) `CHEBI:29035`	UniProtKB:P03965 Source assertion: UniProtKB:P03965 annotates CHEBI:29035 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/75/comments/0/cofactors/1; /results/75/comments/0/note` UniProtKB:P03965 Source assertion: UniProtKB:P03965 has the exact SGD cross-reference SGD:S000003870. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/75/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_CITRUL-BIO2-PWY SGD GO-CAM citrulline biosynthesis - imported from: Saccharomyces Genome Database (source version: Pinned Noctua JSON archive; model date 2024-07-12; member ./YeastPathways_CITRUL-BIO2-PWY.json)
SGD:S000217863 Complex Portal CPX-579: Carbamoyl-phosphate synthase arginine-specific (source version: Inspected API snapshot 2026-10-05; release dates 2014-10-16)
gomodel:YeastPathways_ARGSYNBSUB-PWY GO-CAM L-arginine biosynthesis II acetyl cycle model YeastPathways_ARGSYNBSUB-PWY (source version: Pinned Noctua JSON archive; model date 2026-07-08; member ./YeastPathways_ARGSYNBSUB-PWY.json)
UniProtKB:P07258 Reviewed UniProtKB P07258 SGD cross-reference (source version: UniProt release 2026_03)
UniProtKB:P03965 Reviewed UniProtKB P03965 SGD cross-reference (source version: UniProt release 2026_03)
UniProtKB:P05150 Reviewed UniProtKB P05150 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger. Reviewed exact Complex Portal composition, source evidence grades, and independent physical complex locations.
2026-10-05T06:17:04Z Codex; review-complex-composition; Reviewed exact Complex Portal composition, source evidence grades, and independent physical complex locations; reused existing proteins through reviewed UniProt SGD cross-references.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 2 activity location assignments and 3 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 3 independently supported location edges and quarantined 0 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
Read this curation session