L-lysine biosynthesis IV

gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2

L-lysine biosynthesis IV in Saccharomyces cerevisiae S288C routes acetyl-CoA and 2-oxoglutarate through homocitrate, homoaconitate, 2-oxoadipate, 2-aminoadipate, saccharopine, and L-2-aminoadipate 6-semialdehyde chemistry to L-lysine. Homocitrate dehydration is assigned to ACO2 with a minor ACO1 contribution; LYS4 catalyzes the following homoaconitate hydration.

Pathway type

amino acid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

48 nodes · 89 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
coenzyme A(4-) `CHEBI:57287`	small molecule
2-oxoadipate(2-) `CHEBI:57499`	small molecule
(-)-homoisocitrate(3-) `CHEBI:15404`	small molecule
but-1-ene-1,2,4-tricarboxylate `CHEBI:58174`	small molecule
2-oxoglutarate(2-) `CHEBI:16810`	small molecule
water `CHEBI:15377`	small molecule
LYS4 Scer `SGD:S000002642`	protein
acetyl-CoA(4-) `CHEBI:57288`	small molecule
L-2-aminoadipate(1-) `CHEBI:58672`	small molecule
L-saccharopinate(1-) `CHEBI:57951`	small molecule
cytosol `GO:0005829`	cellular component
L-lysine biosynthetic process `GO:0009085`	biological process
L-glutamate(1-) `CHEBI:29985`	small molecule
carbon dioxide `CHEBI:16526`	small molecule
(2R)-homocitrate(3-) `CHEBI:58884`	small molecule
L-allysine zwitterion `CHEBI:58321`	small molecule
hydron `CHEBI:15378`	small molecule
NADH(2-) `CHEBI:57945`	small molecule
NAD(1-) `CHEBI:57540`	small molecule
LYS12 Scer `SGD:S000001356`	protein
NADPH(4-) `CHEBI:57783`	small molecule
NADP(3-) `CHEBI:58349`	small molecule
LYS1 Scer `SGD:S000001473`	protein
LYS21 Scer `SGD:S000002289`	protein
LYS9 Scer `SGD:S000005333`	protein
LYS2 Scer `SGD:S000000319`	protein
LYS20 Scer `SGD:S000002341`	protein
L-lysinium(1+) `CHEBI:32551`	small molecule
ARO8 Scer `SGD:S000003170`	protein
mitochondrion `GO:0005739`	cellular component
peroxisome `GO:0005777`	cellular component
cytoplasm `GO:0005737`	cellular component
ACO2 Scer `SGD:S000003736`	protein
ACO1 Scer `SGD:S000004295`	protein
tetra-mu3-sulfido-tetrairon `CHEBI:49883`	cofactor
magnesium(2+) `CHEBI:18420`	cofactor
manganese(2+) `CHEBI:29035`	cofactor
pantetheine 4'-phosphate(2-) `CHEBI:47942`	cofactor
pyridoxal 5'-phosphate(2-) `CHEBI:597326`	cofactor
saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	molecular activity
L-2-aminoadipate:2-oxoglutarate transaminase activity `gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN`	molecular activity
homoaconitate hydratase activity `gomodel:HOMOACONITATE-HYDRATASE-RXN`	molecular activity
homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	molecular activity
homoisocitrate dehydrogenase activity `gomodel:RXN-7970`	molecular activity
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	molecular activity
homocitrate dehydratase activity `gomodel:RXN3O-1983`	molecular activity
L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	molecular activity
homocitrate synthase activity `gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOCITRATE-SYNTHASE-RXN RO:0002234 gomodel:CHEBI_57287_HOMOCITRATE-SYNTHASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/0; endpoint types: /individuals/35/type; /individuals/18/type`
L-2-aminoadipate:2-oxoglutarate transaminase activity `gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN`	has_input	2-oxoadipate(2-) `CHEBI:57499`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN RO:0002233 gomodel:CHEBI_57499_RXN-7970. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/1; endpoint types: /individuals/112/type; /individuals/20/type`
homoaconitate hydratase activity `gomodel:HOMOACONITATE-HYDRATASE-RXN`	has_output	(-)-homoisocitrate(3-) `CHEBI:15404`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOACONITATE-HYDRATASE-RXN RO:0002234 gomodel:CHEBI_15404_HOMOACONITATE-HYDRATASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/2; endpoint types: /individuals/34/type; /individuals/10/type`
homocitrate synthase activity `gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076 RO:0002234 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001080. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/3; endpoint types: /individuals/114/type; /individuals/118/type`
homocitrate dehydratase activity `gomodel:RXN3O-1983`	has_output	but-1-ene-1,2,4-tricarboxylate `CHEBI:58174`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-1983 RO:0002234 gomodel:CHEBI_58174_RXN3O-1983. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/4; endpoint types: /individuals/39/type; /individuals/28/type`
L-2-aminoadipate:2-oxoglutarate transaminase activity `gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN`	has_output	2-oxoglutarate(2-) `CHEBI:16810`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN RO:0002234 gomodel:CHEBI_16810_2-AMINOADIPATE-AMINOTRANSFERASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/5; endpoint types: /individuals/112/type; /individuals/13/type`
L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-9808 RO:0002234 gomodel:CHEBI_15377_RXN3O-9808. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/6; endpoint types: /individuals/40/type; /individuals/5/type`
homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	has_input	2-oxoglutarate(2-) `CHEBI:16810`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOCITRATE-SYNTHASE-RXN RO:0002233 gomodel:CHEBI_16810_HOMOCITRATE-SYNTHASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/7; endpoint types: /individuals/35/type; /individuals/14/type`
homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	has_input	acetyl-CoA(4-) `CHEBI:57288`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOCITRATE-SYNTHASE-RXN RO:0002233 gomodel:CHEBI_57288_HOMOCITRATE-SYNTHASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/9; endpoint types: /individuals/35/type; /individuals/19/type`
homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOCITRATE-SYNTHASE-RXN RO:0002233 gomodel:CHEBI_15377_HOMOCITRATE-SYNTHASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/10; endpoint types: /individuals/35/type; /individuals/2/type`
L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	has_input	L-2-aminoadipate(1-) `CHEBI:58672`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-9808 RO:0002233 gomodel:CHEBI_58672_2-AMINOADIPATE-AMINOTRANSFERASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/11; endpoint types: /individuals/40/type; /individuals/32/type`
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 RO:0002234 gomodel:CHEBI_15377_RXN3O-127. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/12; endpoint types: /individuals/38/type; /individuals/3/type`
saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	has_input	L-saccharopinate(1-) `CHEBI:57951`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:1.5.1.7-RXN RO:0002233 gomodel:CHEBI_57951_RXN3O-127. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/13; endpoint types: /individuals/111/type; /individuals/27/type`
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	has_output	L-saccharopinate(1-) `CHEBI:57951`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 RO:0002234 gomodel:CHEBI_57951_RXN3O-127. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/15; endpoint types: /individuals/38/type; /individuals/27/type`
homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	part_of	L-lysine biosynthetic process `GO:0009085`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOCITRATE-SYNTHASE-RXN BFO:0000050 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/YeastPathways_LYSINE-AMINOAD-PWY-2. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/17; endpoint types: /individuals/35/type; /individuals/113/type`
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	has_input	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 RO:0002233 gomodel:CHEBI_29985_RXN3O-127. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/18; endpoint types: /individuals/38/type; /individuals/16/type`
homoisocitrate dehydrogenase activity `gomodel:RXN-7970`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN-7970 RO:0002234 gomodel:CHEBI_16526_RXN-7970. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/19; endpoint types: /individuals/37/type; /individuals/11/type`
homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	has_output	(2R)-homocitrate(3-) `CHEBI:58884`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOCITRATE-SYNTHASE-RXN RO:0002234 gomodel:CHEBI_58884_HOMOCITRATE-SYNTHASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/20; endpoint types: /individuals/35/type; /individuals/33/type`
L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	has_output	L-allysine zwitterion `CHEBI:58321`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-9808 RO:0002234 gomodel:CHEBI_58321_RXN3O-9808. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/21; endpoint types: /individuals/40/type; /individuals/29/type`
saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:1.5.1.7-RXN RO:0002234 gomodel:CHEBI_15378_1.5.1.7-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/23; endpoint types: /individuals/111/type; /individuals/6/type`
homoisocitrate dehydrogenase activity `gomodel:RXN-7970`	has_output	NADH(2-) `CHEBI:57945`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN-7970 RO:0002234 gomodel:CHEBI_57945_RXN-7970. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/24; endpoint types: /individuals/37/type; /individuals/26/type`
L-2-aminoadipate:2-oxoglutarate transaminase activity `gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN BFO:0000066 gomodel:reaction_2-AMINOADIPATE-AMINOTRANSFERASE-RXN_location_lociGO_0005829. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/25; endpoint types: /individuals/112/type; /individuals/104/type`
LYS4 Scer `SGD:S000002642`	enables	homoaconitate hydratase activity `gomodel:HOMOACONITATE-HYDRATASE-RXN`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOACONITATE-HYDRATASE-RXN RO:0002333 gomodel:YDR234W-MONOMER_HOMOACONITATE-HYDRATASE-RXN_controller. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/26; endpoint types: /individuals/34/type; /individuals/43/type`
homoisocitrate dehydrogenase activity `gomodel:RXN-7970`	has_input	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN-7970 RO:0002233 gomodel:CHEBI_57540_RXN-7970. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/27; endpoint types: /individuals/37/type; /individuals/22/type`
homocitrate synthase activity `gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076`	has_input	2-oxoglutarate(2-) `CHEBI:16810`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076 RO:0002233 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001077. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/28; endpoint types: /individuals/114/type; /individuals/115/type`
LYS12 Scer `SGD:S000001356`	enables	homoisocitrate dehydrogenase activity `gomodel:RXN-7970`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN-7970 RO:0002333 gomodel:YIL094C-MONOMER_RXN-7970_controller. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/29; endpoint types: /individuals/37/type; /individuals/45/type`
homocitrate synthase activity `gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076`	has_output	(2R)-homocitrate(3-) `CHEBI:58884`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076 RO:0002234 gomodel:CHEBI_58884_HOMOCITRATE-SYNTHASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/30; endpoint types: /individuals/114/type; /individuals/33/type`
homocitrate dehydratase activity `gomodel:RXN3O-1983`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-1983 RO:0002234 gomodel:CHEBI_15377_RXN3O-1983. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/31; endpoint types: /individuals/39/type; /individuals/4/type`
L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-9808 RO:0002233 gomodel:CHEBI_57783_RXN3O-9808. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/32; endpoint types: /individuals/40/type; /individuals/24/type`
L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	part_of	L-lysine biosynthetic process `GO:0009085`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-9808 BFO:0000050 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/YeastPathways_LYSINE-AMINOAD-PWY-2. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/33; endpoint types: /individuals/40/type; /individuals/113/type`
homoisocitrate dehydrogenase activity `gomodel:RXN-7970`	part_of	L-lysine biosynthetic process `GO:0009085`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN-7970 BFO:0000050 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/YeastPathways_LYSINE-AMINOAD-PWY-2. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/34; endpoint types: /individuals/37/type; /individuals/113/type`
saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	part_of	L-lysine biosynthetic process `GO:0009085`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:1.5.1.7-RXN BFO:0000050 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/YeastPathways_LYSINE-AMINOAD-PWY-2. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/35; endpoint types: /individuals/111/type; /individuals/113/type`
L-2-aminoadipate:2-oxoglutarate transaminase activity `gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN`	has_output	L-2-aminoadipate(1-) `CHEBI:58672`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN RO:0002234 gomodel:CHEBI_58672_2-AMINOADIPATE-AMINOTRANSFERASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/36; endpoint types: /individuals/112/type; /individuals/32/type`
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	part_of	L-lysine biosynthetic process `GO:0009085`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 BFO:0000050 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/YeastPathways_LYSINE-AMINOAD-PWY-2. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/37; endpoint types: /individuals/38/type; /individuals/113/type`
L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-9808 RO:0002234 gomodel:CHEBI_58349_RXN3O-9808. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/38; endpoint types: /individuals/40/type; /individuals/31/type`
homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOCITRATE-SYNTHASE-RXN BFO:0000066 gomodel:reaction_HOMOCITRATE-SYNTHASE-RXN_location_lociGO_0005829. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/39; endpoint types: /individuals/35/type; /individuals/106/type`
homocitrate synthase activity `gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076 RO:0002233 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001079. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/40; endpoint types: /individuals/114/type; /individuals/117/type`
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 RO:0002233 gomodel:CHEBI_15378_RXN3O-127. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/41; endpoint types: /individuals/38/type; /individuals/8/type`
homocitrate synthase activity `gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076`	part_of	L-lysine biosynthetic process `GO:0009085`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076 BFO:0000050 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/YeastPathways_LYSINE-AMINOAD-PWY-2. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/42; endpoint types: /individuals/114/type; /individuals/113/type`
homocitrate synthase activity `gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076 RO:0002234 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001081. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/43; endpoint types: /individuals/114/type; /individuals/119/type`
LYS1 Scer `SGD:S000001473`	enables	saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:1.5.1.7-RXN RO:0002333 gomodel:YIR034C-MONOMER_1.5.1.7-RXN_controller. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/44; endpoint types: /individuals/111/type; /individuals/46/type`
homoaconitate hydratase activity `gomodel:HOMOACONITATE-HYDRATASE-RXN`	part_of	L-lysine biosynthetic process `GO:0009085`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOACONITATE-HYDRATASE-RXN BFO:0000050 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/YeastPathways_LYSINE-AMINOAD-PWY-2. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/45; endpoint types: /individuals/34/type; /individuals/113/type`
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 BFO:0000066 gomodel:reaction_RXN3O-127_location_lociGO_0005829. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/46; endpoint types: /individuals/38/type; /individuals/108/type`
L-2-aminoadipate:2-oxoglutarate transaminase activity `gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN`	has_input	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN RO:0002233 gomodel:CHEBI_29985_2-AMINOADIPATE-AMINOTRANSFERASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/48; endpoint types: /individuals/112/type; /individuals/15/type`
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	has_input	L-allysine zwitterion `CHEBI:58321`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 RO:0002233 gomodel:CHEBI_58321_RXN3O-9808. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/49; endpoint types: /individuals/38/type; /individuals/29/type`
homoisocitrate dehydrogenase activity `gomodel:RXN-7970`	has_output	2-oxoadipate(2-) `CHEBI:57499`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN-7970 RO:0002234 gomodel:CHEBI_57499_RXN-7970. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/50; endpoint types: /individuals/37/type; /individuals/20/type`
LYS21 Scer `SGD:S000002289`	enables	homocitrate synthase activity `gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076 RO:0002333 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001082. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/51; endpoint types: /individuals/114/type; /individuals/120/type`
homoisocitrate dehydrogenase activity `gomodel:RXN-7970`	has_input	(-)-homoisocitrate(3-) `CHEBI:15404`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN-7970 RO:0002233 gomodel:CHEBI_15404_HOMOACONITATE-HYDRATASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/52; endpoint types: /individuals/37/type; /individuals/10/type`
saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	has_output	2-oxoglutarate(2-) `CHEBI:16810`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:1.5.1.7-RXN RO:0002234 gomodel:CHEBI_16810_1.5.1.7-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/53; endpoint types: /individuals/111/type; /individuals/12/type`
L-2-aminoadipate:2-oxoglutarate transaminase activity `gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN`	part_of	L-lysine biosynthetic process `GO:0009085`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN BFO:0000050 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/YeastPathways_LYSINE-AMINOAD-PWY-2. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/54; endpoint types: /individuals/112/type; /individuals/113/type`
L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-9808 RO:0002233 gomodel:CHEBI_15378_RXN3O-9808. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/55; endpoint types: /individuals/40/type; /individuals/9/type`
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 RO:0002234 gomodel:CHEBI_58349_RXN3O-127. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/56; endpoint types: /individuals/38/type; /individuals/30/type`
LYS9 Scer `SGD:S000005333`	enables	saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 RO:0002333 gomodel:MONOMER3O-363_RXN3O-127_controller. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/58; endpoint types: /individuals/38/type; /individuals/36/type`
homocitrate synthase activity `gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076`	has_input	acetyl-CoA(4-) `CHEBI:57288`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001076 RO:0002233 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/6a4c244800001078. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/59; endpoint types: /individuals/114/type; /individuals/116/type`
LYS2 Scer `SGD:S000000319`	enables	L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-9808 RO:0002333 gomodel:YBR115C-MONOMER_RXN3O-9808_controller. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/60; endpoint types: /individuals/40/type; /individuals/41/type`
LYS20 Scer `SGD:S000002341`	enables	homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOCITRATE-SYNTHASE-RXN RO:0002333 gomodel:YDL182W-MONOMER_HOMOCITRATE-SYNTHASE-RXN_controller. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/61; endpoint types: /individuals/35/type; /individuals/42/type`
saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	has_output	L-lysinium(1+) `CHEBI:32551`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:1.5.1.7-RXN RO:0002234 gomodel:CHEBI_32551_1.5.1.7-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/62; endpoint types: /individuals/111/type; /individuals/17/type`
saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity `gomodel:RXN3O-127`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-127 RO:0002233 gomodel:CHEBI_57783_RXN3O-127. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/63; endpoint types: /individuals/38/type; /individuals/23/type`
homocitrate synthase activity `gomodel:HOMOCITRATE-SYNTHASE-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOCITRATE-SYNTHASE-RXN RO:0002234 gomodel:CHEBI_15378_HOMOCITRATE-SYNTHASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/64; endpoint types: /individuals/35/type; /individuals/7/type`
homoaconitate hydratase activity `gomodel:HOMOACONITATE-HYDRATASE-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOACONITATE-HYDRATASE-RXN RO:0002233 gomodel:CHEBI_15377_HOMOACONITATE-HYDRATASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/65; endpoint types: /individuals/34/type; /individuals/1/type`
ARO8 Scer `SGD:S000003170`	enables	L-2-aminoadipate:2-oxoglutarate transaminase activity `gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:2-AMINOADIPATE-AMINOTRANSFERASE-RXN RO:0002333 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/67c10cc400000549. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/66; endpoint types: /individuals/112/type; /individuals/131/type`
homocitrate dehydratase activity `gomodel:RXN3O-1983`	part_of	L-lysine biosynthetic process `GO:0009085`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-1983 BFO:0000050 gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2/YeastPathways_LYSINE-AMINOAD-PWY-2. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/67; endpoint types: /individuals/39/type; /individuals/113/type`
L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity `gomodel:RXN3O-9808`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-9808 BFO:0000066 gomodel:reaction_RXN3O-9808_location_lociGO_0005829. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/68; endpoint types: /individuals/40/type; /individuals/110/type`
homocitrate dehydratase activity `gomodel:RXN3O-1983`	has_input	(2R)-homocitrate(3-) `CHEBI:58884`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:RXN3O-1983 RO:0002233 gomodel:CHEBI_58884_HOMOCITRATE-SYNTHASE-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/69; endpoint types: /individuals/39/type; /individuals/33/type`
saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	has_output	NADH(2-) `CHEBI:57945`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:1.5.1.7-RXN RO:0002234 gomodel:CHEBI_57945_1.5.1.7-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/70; endpoint types: /individuals/111/type; /individuals/25/type`
saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:1.5.1.7-RXN RO:0002233 gomodel:CHEBI_15377_1.5.1.7-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/71; endpoint types: /individuals/111/type; /individuals/0/type`
homoaconitate hydratase activity `gomodel:HOMOACONITATE-HYDRATASE-RXN`	has_input	but-1-ene-1,2,4-tricarboxylate `CHEBI:58174`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:HOMOACONITATE-HYDRATASE-RXN RO:0002233 gomodel:CHEBI_58174_RXN3O-1983. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/72; endpoint types: /individuals/34/type; /individuals/28/type`
saccharopine dehydrogenase (NAD+, L-lysine-forming) activity `gomodel:1.5.1.7-RXN`	has_input	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 Source assertion: The model asserts gomodel:1.5.1.7-RXN RO:0002233 gomodel:CHEBI_57540_1.5.1.7-RXN. Source location `./YeastPathways_LYSINE-AMINOAD-PWY-2.json#/facts/73; endpoint types: /individuals/111/type; /individuals/21/type`
LYS4 Scer `SGD:S000002642`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	mitochondrion `GO:0005739`	UniProtKB:P49367 Source assertion: UniProtKB:P49367 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3908/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P49367 Source assertion: UniProtKB:P49367 has the exact SGD cross-reference SGD:S000002642. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3908/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
LYS12 Scer `SGD:S000001356`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:14576278)	mitochondrion `GO:0005739`	UniProtKB:P40495 Source assertion: UniProtKB:P40495 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1737/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P40495 Source assertion: UniProtKB:P40495 has the exact SGD cross-reference SGD:S000001356. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1737/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
LYS1 Scer `SGD:S000001473`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:26928762); ECO:0000269 (PubMed:28928432)	peroxisome `GO:0005777`	UniProtKB:P38998 Source assertion: UniProtKB:P38998 annotates location Peroxisome (SL-0204). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1534/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P38998 Source assertion: UniProtKB:P38998 has the exact SGD cross-reference SGD:S000001473. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1534/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
LYS21 Scer `SGD:S000002289`	located_in Physical protein location from reviewed UniProt. ECO:0000305	mitochondrion `GO:0005739`	UniProtKB:Q12122 Source assertion: UniProtKB:Q12122 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4309/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q12122 Source assertion: UniProtKB:Q12122 has the exact SGD cross-reference SGD:S000002289. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4309/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
LYS20 Scer `SGD:S000002341`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	cytoplasm `GO:0005737`	UniProtKB:P48570 Source assertion: UniProtKB:P48570 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3903/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P48570 Source assertion: UniProtKB:P48570 has the exact SGD cross-reference SGD:S000002341. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3903/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ARO8 Scer `SGD:S000003170`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P53090 Source assertion: UniProtKB:P53090 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2112/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P53090 Source assertion: UniProtKB:P53090 has the exact SGD cross-reference SGD:S000003170. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2112/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ACO2 Scer `SGD:S000003736`	enables PMID:23106124 separates homocitrate dehydration (ACO2, with minor ACO1 contribution) from homoaconitate hydration (LYS4).	homocitrate dehydratase activity `gomodel:RXN3O-1983`	UniProtKB:P39533 Source assertion: UniProt P39533 assigns reversible (R)-homocitrate dehydration to cis-homoaconitate to ACO2, supported by PMID:23106124. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/1568/comments/0` UniProtKB:P39533 Source assertion: UniProt P39533 has the exact SGD cross-reference SGD:S000003736. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/1568/uniProtKBCrossReferences`
ACO2 Scer `SGD:S000003736`	located_in Physical protein location; does not place every activity of this enzyme in this compartment. ACO1 is mainly mitochondrial.	mitochondrion `GO:0005739`	UniProtKB:P39533 Source assertion: UniProt P39533 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d] #/results/1568/comments/1/subcellularLocations/0`
ACO1 Scer `SGD:S000004295`	enables PMID:23106124 separates homocitrate dehydration (ACO2, with minor ACO1 contribution) from homoaconitate hydration (LYS4).	homocitrate dehydratase activity `gomodel:RXN3O-1983`	UniProtKB:P19414 Source assertion: UniProt P19414 assigns a minor contribution to reversible (R)-homocitrate dehydration to ACO1, supported by PMID:23106124. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/497/comments/0` UniProtKB:P19414 Source assertion: UniProt P19414 has the exact SGD cross-reference SGD:S000004295. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/497/uniProtKBCrossReferences`
ACO1 Scer `SGD:S000004295`	located_in Physical protein location; does not place every activity of this enzyme in this compartment. ACO1 is mainly mitochondrial.	mitochondrion `GO:0005739`	UniProtKB:P19414 Source assertion: UniProt P19414 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d] #/results/497/comments/1/subcellularLocations/0`
ACO1 Scer `SGD:S000004295`	located_in Physical protein location; does not place every activity of this enzyme in this compartment. ACO1 is mainly mitochondrial.	cytoplasm `GO:0005737`	UniProtKB:P19414 Source assertion: UniProt P19414 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d] #/results/497/comments/1/subcellularLocations/1`
LYS4 Scer `SGD:S000002642`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 [4Fe-4S] cluster per subunit. UniProt note evidence: ECO:0000250	tetra-mu3-sulfido-tetrairon `CHEBI:49883`	UniProtKB:P49367 Source assertion: UniProtKB:P49367 annotates CHEBI:49883 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3908/comments/0/cofactors/0; /results/3908/comments/0/note` UniProtKB:P49367 Source assertion: UniProtKB:P49367 has the exact SGD cross-reference SGD:S000002642. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3908/uniProtKBCrossReferences/0`
LYS12 Scer `SGD:S000001356`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:17223711); ECO:0000269 (PubMed:19530703) Binds 1 Mg(2+) ion per subunit. UniProt note evidence: ECO:0000269 (PubMed:17223711); ECO:0000269 (PubMed:19530703)	magnesium(2+) `CHEBI:18420`	UniProtKB:P40495 Source assertion: UniProtKB:P40495 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1737/comments/0/cofactors/0; /results/1737/comments/0/note` UniProtKB:P40495 Source assertion: UniProtKB:P40495 has the exact SGD cross-reference SGD:S000001356. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1737/uniProtKBCrossReferences/0`
LYS21 Scer `SGD:S000002289`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:O87198)	magnesium(2+) `CHEBI:18420`	UniProtKB:Q12122 Source assertion: UniProtKB:Q12122 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4309/comments/0/cofactors/0` UniProtKB:Q12122 Source assertion: UniProtKB:Q12122 has the exact SGD cross-reference SGD:S000002289. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4309/uniProtKBCrossReferences/0`
LYS21 Scer `SGD:S000002289`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:O87198)	manganese(2+) `CHEBI:29035`	UniProtKB:Q12122 Source assertion: UniProtKB:Q12122 annotates CHEBI:29035 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4309/comments/0/cofactors/1` UniProtKB:Q12122 Source assertion: UniProtKB:Q12122 has the exact SGD cross-reference SGD:S000002289. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4309/uniProtKBCrossReferences/0`
LYS2 Scer `SGD:S000000319`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. Reviewed source qualification: The source describes a covalently attached cofactor moiety. Keep that bound-state qualification; no free cosubstrate consumption is inferred. UniProt evidence: ECO:0000269 (PubMed:10320345) Binds 1 phosphopantetheine covalently. UniProt note evidence: ECO:0000269 (PubMed:10320345)	pantetheine 4'-phosphate(2-) `CHEBI:47942`	UniProtKB:P07702 Source assertion: UniProtKB:P07702 annotates CHEBI:47942 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/193/comments/0/cofactors/0; /results/193/comments/0/note` UniProtKB:P07702 Source assertion: UniProtKB:P07702 has the exact SGD cross-reference SGD:S000000319. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/193/uniProtKBCrossReferences/0`
LYS20 Scer `SGD:S000002341`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:O87198)	magnesium(2+) `CHEBI:18420`	UniProtKB:P48570 Source assertion: UniProtKB:P48570 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3903/comments/0/cofactors/0` UniProtKB:P48570 Source assertion: UniProtKB:P48570 has the exact SGD cross-reference SGD:S000002341. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3903/uniProtKBCrossReferences/0`
LYS20 Scer `SGD:S000002341`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:O87198)	manganese(2+) `CHEBI:29035`	UniProtKB:P48570 Source assertion: UniProtKB:P48570 annotates CHEBI:29035 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3903/comments/0/cofactors/1` UniProtKB:P48570 Source assertion: UniProtKB:P48570 has the exact SGD cross-reference SGD:S000002341. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3903/uniProtKBCrossReferences/0`
ARO8 Scer `SGD:S000003170`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:23893908)	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P53090 Source assertion: UniProtKB:P53090 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2112/comments/0/cofactors/0` UniProtKB:P53090 Source assertion: UniProtKB:P53090 has the exact SGD cross-reference SGD:S000003170. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2112/uniProtKBCrossReferences/0`
ACO2 Scer `SGD:S000003736`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P20004) Binds 1 [4Fe-4S] cluster per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P20004)	tetra-mu3-sulfido-tetrairon `CHEBI:49883`	UniProtKB:P39533 Source assertion: UniProtKB:P39533 annotates CHEBI:49883 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1568/comments/0/cofactors/0; /results/1568/comments/0/note` UniProtKB:P39533 Source assertion: UniProtKB:P39533 has the exact SGD cross-reference SGD:S000003736. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1568/uniProtKBCrossReferences/0`
ACO1 Scer `SGD:S000004295`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:27738674) Binds 1 [4Fe-4S] cluster per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P20004)	tetra-mu3-sulfido-tetrairon `CHEBI:49883`	UniProtKB:P19414 Source assertion: UniProtKB:P19414 annotates CHEBI:49883 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/497/comments/0/cofactors/0; /results/497/comments/0/note` UniProtKB:P19414 Source assertion: UniProtKB:P19414 has the exact SGD cross-reference SGD:S000004295. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/497/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_LYSINE-AMINOAD-PWY-2 SGD GO-CAM L-lysine biosynthesis IV model (source version: Pinned Noctua JSON archive; model date 2026-07-10; member ./YeastPathways_LYSINE-AMINOAD-PWY-2.json)
UniProtKB:P49367 Reviewed UniProtKB P49367 location annotations (source version: UniProt release 2026_03)
UniProtKB:P40495 Reviewed UniProtKB P40495 location annotations (source version: UniProt release 2026_03)
UniProtKB:P38998 Reviewed UniProtKB P38998 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q12122 Reviewed UniProtKB Q12122 location annotations (source version: UniProt release 2026_03)
UniProtKB:P48570 Reviewed UniProtKB P48570 location annotations (source version: UniProt release 2026_03)
UniProtKB:P53090 Reviewed UniProtKB P53090 location annotations (source version: UniProt release 2026_03)
UniProtKB:P39533 Reviewed UniProt P39533: catalytic-function assertions (source version: 2026_03)
UniProtKB:P19414 Reviewed UniProt P19414: catalytic-function assertions (source version: 2026_03)
UniProtKB:P07702 UniProtKB P07702 cofactor annotations (source version: 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 9 activity location assignments and 8 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 6 independently supported location edges and quarantined 5 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
Read this curation session
2026-10-05T06:58:25Z claude-code
- EDIT — Correct homocitrate dehydration enzyme assignment
  Inspected complete primary paper PMID23106124 and exact current UniProt functions. Replaced LYS4 homocitrate-dehydration assignment with ACO2 and explicitly minor ACO1 contribution; retained LYS4 homoaconitate hydration and added independently annotated physical compartments. Source conflict ledger: reports/causal_graph_review/gocam-assignment-conflicts.json. Schema validation and idempotent dry-run passed.
Read this curation session