formaldehyde oxidation II (glutathione-dependent)

gomodel:YeastPathways_PWY-1801-1

Formaldehyde oxidation II in Saccharomyces cerevisiae S288C condenses formaldehyde with glutathione, oxidizes S-(hydroxymethyl)glutathione to S-formylglutathione, hydrolyzes S-formylglutathione to formate and glutathione, and oxidizes formate to carbon dioxide.

Pathway type

formaldehyde degradation

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

25 nodes · 37 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
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Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
NAD(P)(+) `CHEBI:13390`	small molecule
S-(hydroxymethyl)glutathione(1-) `CHEBI:58758`	small molecule
water `CHEBI:15377`	small molecule
NAD(1-) `CHEBI:57540`	small molecule
S-formylglutathionate(1-) `CHEBI:57688`	small molecule
carbon dioxide `CHEBI:16526`	small molecule
NADH(2-) `CHEBI:57945`	small molecule
information biomacromolecule `gomodel:YeastPathways_PWY-1801-1/65692e7e00001183`	participant
hydron `CHEBI:15378`	small molecule
glutathionate(1-) `CHEBI:57925`	small molecule
formaldehyde catabolic process `GO:0046294`	biological process
FDH1 Scer `SGD:S000005915`	protein
SFA1 Scer `SGD:S000002327`	protein
cytosol `GO:0005829`	cellular component
formaldehyde `CHEBI:16842`	small molecule
cytoplasm `GO:0005737`	cellular component
YJL068C Scer `SGD:S000003604`	protein
NAD(P)H `CHEBI:13392`	small molecule
formate `CHEBI:15740`	small molecule
mitochondrion `GO:0005739`	cellular component
zinc(2+) `CHEBI:29105`	cofactor
formate dehydrogenase (NAD+) activity `gomodel:1.2.1.2-RXN`	molecular activity
S-(hydroxymethyl)glutathione synthase activity `gomodel:RXN-2961`	molecular activity
S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	molecular activity
S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	has_input	NAD(P)(+) `CHEBI:13390`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2962 RO:0002233 gomodel:CHEBI_13390_RXN-2962. Source location `./YeastPathways_PWY-1801-1.json#/facts/0; endpoint types: /individuals/15/type; /individuals/0/type`
S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	has_input	S-(hydroxymethyl)glutathione(1-) `CHEBI:58758`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2962 RO:0002233 gomodel:CHEBI_58758_RXN-2961. Source location `./YeastPathways_PWY-1801-1.json#/facts/1; endpoint types: /individuals/15/type; /individuals/13/type`
S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN RO:0002233 gomodel:CHEBI_15377_S-FORMYLGLUTATHIONE-HYDROLASE-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/2; endpoint types: /individuals/16/type; /individuals/2/type`
formate dehydrogenase (NAD+) activity `gomodel:1.2.1.2-RXN`	has_input	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:1.2.1.2-RXN RO:0002233 gomodel:CHEBI_57540_1.2.1.2-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/3; endpoint types: /individuals/48/type; /individuals/8/type`
S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	has_output	S-formylglutathionate(1-) `CHEBI:57688`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2962 RO:0002234 gomodel:CHEBI_57688_RXN-2962. Source location `./YeastPathways_PWY-1801-1.json#/facts/4; endpoint types: /individuals/15/type; /individuals/9/type`
formate dehydrogenase (NAD+) activity `gomodel:1.2.1.2-RXN`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:1.2.1.2-RXN RO:0002234 gomodel:CHEBI_16526_1.2.1.2-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/5; endpoint types: /individuals/48/type; /individuals/6/type`
formate dehydrogenase (NAD+) activity `gomodel:1.2.1.2-RXN`	has_output	NADH(2-) `CHEBI:57945`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:1.2.1.2-RXN RO:0002234 gomodel:CHEBI_57945_1.2.1.2-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/6; endpoint types: /individuals/48/type; /individuals/12/type`
information biomacromolecule `gomodel:YeastPathways_PWY-1801-1/65692e7e00001183`	enables	S-(hydroxymethyl)glutathione synthase activity `gomodel:RXN-2961`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2961 RO:0002333 gomodel:YeastPathways_PWY-1801-1/65692e7e00001183. Source location `./YeastPathways_PWY-1801-1.json#/facts/7; endpoint types: /individuals/14/type; /individuals/53/type`
S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN RO:0002234 gomodel:CHEBI_15378_S-FORMYLGLUTATHIONE-HYDROLASE-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/8; endpoint types: /individuals/16/type; /individuals/4/type`
S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	has_output	glutathionate(1-) `CHEBI:57925`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN RO:0002234 gomodel:CHEBI_57925_S-FORMYLGLUTATHIONE-HYDROLASE-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/9; endpoint types: /individuals/16/type; /individuals/11/type`
S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	part_of	formaldehyde catabolic process `GO:0046294`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2962 BFO:0000050 gomodel:YeastPathways_PWY-1801-1/YeastPathways_PWY-1801-1. Source location `./YeastPathways_PWY-1801-1.json#/facts/10; endpoint types: /individuals/15/type; /individuals/49/type`
FDH1 Scer `SGD:S000005915`	enables	formate dehydrogenase (NAD+) activity `gomodel:1.2.1.2-RXN`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:1.2.1.2-RXN RO:0002333 gomodel:YOR388C-MONOMER_1.2.1.2-RXN_controller. Source location `./YeastPathways_PWY-1801-1.json#/facts/11; endpoint types: /individuals/48/type; /individuals/19/type`
SFA1 Scer `SGD:S000002327`	enables	S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2962 RO:0002333 gomodel:YDL168W-MONOMER_RXN-2962_controller. Source location `./YeastPathways_PWY-1801-1.json#/facts/12; endpoint types: /individuals/15/type; /individuals/17/type`
S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2962 RO:0002234 gomodel:CHEBI_15378_RXN-2962. Source location `./YeastPathways_PWY-1801-1.json#/facts/13; endpoint types: /individuals/15/type; /individuals/3/type`
S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN BFO:0000066 gomodel:reaction_S-FORMYLGLUTATHIONE-HYDROLASE-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-1801-1.json#/facts/14; endpoint types: /individuals/16/type; /individuals/47/type`
formate dehydrogenase (NAD+) activity `gomodel:1.2.1.2-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:1.2.1.2-RXN BFO:0000066 gomodel:reaction_1.2.1.2-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-1801-1.json#/facts/15; endpoint types: /individuals/48/type; /individuals/44/type`
S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	part_of	formaldehyde catabolic process `GO:0046294`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN BFO:0000050 gomodel:YeastPathways_PWY-1801-1/YeastPathways_PWY-1801-1. Source location `./YeastPathways_PWY-1801-1.json#/facts/16; endpoint types: /individuals/16/type; /individuals/49/type`
S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	provides_input_for	S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2962 RO:0002413 gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/17; endpoint types: /individuals/15/type; /individuals/16/type`
formate dehydrogenase (NAD+) activity `gomodel:1.2.1.2-RXN`	part_of	formaldehyde catabolic process `GO:0046294`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:1.2.1.2-RXN BFO:0000050 gomodel:YeastPathways_PWY-1801-1/YeastPathways_PWY-1801-1. Source location `./YeastPathways_PWY-1801-1.json#/facts/18; endpoint types: /individuals/48/type; /individuals/49/type`
S-(hydroxymethyl)glutathione synthase activity `gomodel:RXN-2961`	has_input	formaldehyde `CHEBI:16842`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2961 RO:0002233 gomodel:CHEBI_16842_RXN-2961. Source location `./YeastPathways_PWY-1801-1.json#/facts/19; endpoint types: /individuals/14/type; /individuals/7/type`
S-(hydroxymethyl)glutathione synthase activity `gomodel:RXN-2961`	has_input	glutathionate(1-) `CHEBI:57925`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2961 RO:0002233 gomodel:CHEBI_57925_RXN-2961. Source location `./YeastPathways_PWY-1801-1.json#/facts/20; endpoint types: /individuals/14/type; /individuals/10/type`
S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	occurs_in	cytoplasm `GO:0005737`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2962 BFO:0000066 gomodel:reaction_RXN-2962_location_lociGO_0005829. Source location `./YeastPathways_PWY-1801-1.json#/facts/21; endpoint types: /individuals/15/type; /individuals/46/type`
S-(hydroxymethyl)glutathione synthase activity `gomodel:RXN-2961`	has_output	S-(hydroxymethyl)glutathione(1-) `CHEBI:58758`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2961 RO:0002234 gomodel:CHEBI_58758_RXN-2961. Source location `./YeastPathways_PWY-1801-1.json#/facts/22; endpoint types: /individuals/14/type; /individuals/13/type`
YJL068C Scer `SGD:S000003604`	enables	S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN RO:0002333 gomodel:YJL068C-MONOMER_S-FORMYLGLUTATHIONE-HYDROLASE-RXN_controller. Source location `./YeastPathways_PWY-1801-1.json#/facts/23; endpoint types: /individuals/16/type; /individuals/18/type`
S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	has_output	NAD(P)H `CHEBI:13392`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2962 RO:0002234 gomodel:CHEBI_13392_RXN-2962. Source location `./YeastPathways_PWY-1801-1.json#/facts/24; endpoint types: /individuals/15/type; /individuals/1/type`
S-(hydroxymethyl)glutathione synthase activity `gomodel:RXN-2961`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2961 BFO:0000066 gomodel:reaction_RXN-2961_location_lociGO_0005829. Source location `./YeastPathways_PWY-1801-1.json#/facts/25; endpoint types: /individuals/14/type; /individuals/45/type`
S-(hydroxymethyl)glutathione synthase activity `gomodel:RXN-2961`	provides_input_for	S-(hydroxymethyl)glutathione + NAD(P)+ -> S-formylglutathione + NAD(P)H + H+ `gomodel:RXN-2962`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2961 RO:0002413 gomodel:RXN-2962. Source location `./YeastPathways_PWY-1801-1.json#/facts/26; endpoint types: /individuals/14/type; /individuals/15/type`
S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	has_output	formate `CHEBI:15740`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN RO:0002234 gomodel:CHEBI_15740_S-FORMYLGLUTATHIONE-HYDROLASE-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/27; endpoint types: /individuals/16/type; /individuals/5/type`
S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	has_input	S-formylglutathionate(1-) `CHEBI:57688`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN RO:0002233 gomodel:CHEBI_57688_RXN-2962. Source location `./YeastPathways_PWY-1801-1.json#/facts/28; endpoint types: /individuals/16/type; /individuals/9/type`
S-(hydroxymethyl)glutathione synthase activity `gomodel:RXN-2961`	part_of	formaldehyde catabolic process `GO:0046294`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:RXN-2961 BFO:0000050 gomodel:YeastPathways_PWY-1801-1/YeastPathways_PWY-1801-1. Source location `./YeastPathways_PWY-1801-1.json#/facts/29; endpoint types: /individuals/14/type; /individuals/49/type`
S-formylglutathione hydrolase activity `gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN`	provides_input_for	formate dehydrogenase (NAD+) activity `gomodel:1.2.1.2-RXN`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:S-FORMYLGLUTATHIONE-HYDROLASE-RXN RO:0002413 gomodel:1.2.1.2-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/30; endpoint types: /individuals/16/type; /individuals/48/type`
formate dehydrogenase (NAD+) activity `gomodel:1.2.1.2-RXN`	has_input	formate `CHEBI:15740`	gomodel:YeastPathways_PWY-1801-1 Source assertion: The model asserts gomodel:1.2.1.2-RXN RO:0002233 gomodel:CHEBI_15740_S-FORMYLGLUTATHIONE-HYDROLASE-RXN. Source location `./YeastPathways_PWY-1801-1.json#/facts/31; endpoint types: /individuals/48/type; /individuals/5/type`
FDH1 Scer `SGD:S000005915`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03210); ECO:0000269 (PubMed:11921099)	cytoplasm `GO:0005737`	UniProtKB:Q08911 Source assertion: UniProtKB:Q08911 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4271/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q08911 Source assertion: UniProtKB:Q08911 has the exact SGD cross-reference SGD:S000005915. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4271/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
SFA1 Scer `SGD:S000002327`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P32771 Source assertion: UniProtKB:P32771 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/990/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32771 Source assertion: UniProtKB:P32771 has the exact SGD cross-reference SGD:S000002327. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/990/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
SFA1 Scer `SGD:S000002327`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:16823961)	mitochondrion `GO:0005739`	UniProtKB:P32771 Source assertion: UniProtKB:P32771 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/990/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32771 Source assertion: UniProtKB:P32771 has the exact SGD cross-reference SGD:S000002327. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/990/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
YJL068C Scer `SGD:S000003604`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:10427036)	cytoplasm `GO:0005737`	UniProtKB:P40363 Source assertion: UniProtKB:P40363 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3743/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P40363 Source assertion: UniProtKB:P40363 has the exact SGD cross-reference SGD:S000003604. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3743/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
SFA1 Scer `SGD:S000002327`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P06525) Binds 2 Zn(2+) ions per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P06525)	zinc(2+) `CHEBI:29105`	UniProtKB:P32771 Source assertion: UniProtKB:P32771 annotates CHEBI:29105 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/990/comments/0/cofactors/0; /results/990/comments/0/note` UniProtKB:P32771 Source assertion: UniProtKB:P32771 has the exact SGD cross-reference SGD:S000002327. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/990/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWY-1801-1 GO-CAM formaldehyde oxidation II model YeastPathways_PWY-1801-1 (source version: Pinned Noctua JSON archive; model date 2026-08-04; member ./YeastPathways_PWY-1801-1.json)
UniProtKB:Q08911 Reviewed UniProtKB Q08911 location annotations (source version: UniProt release 2026_03)
UniProtKB:P32771 Reviewed UniProtKB P32771 location annotations (source version: UniProt release 2026_03)
UniProtKB:P40363 Reviewed UniProtKB P40363 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 4 activity location assignments and 3 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 4 independently supported location edges and quarantined 0 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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