S-adenosyl-L-methionine cycle II

gomodel:YeastPathways_PWY-5041

The SGD S-adenosyl-L-methionine cycle II GO-CAM for Saccharomyces cerevisiae S288C models MET6-enabled 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase, SAM1- and SAM2-enabled methionine adenosyltransferase, and SAH1-enabled adenosylhomocysteinase activities. The source also models the generic SAM-dependent methyl transfer step without assigning an enabling protein.

Pathway type

amino acid metabolism

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

29 nodes · 45 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
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Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
S-adenosyl-L-methionine zwitterion `CHEBI:59789`	small molecule
cytosol `GO:0005829`	cellular component
L-methionine cycle `GO:0033353`	biological process
L-methionine zwitterion `CHEBI:57844`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
adenosine `CHEBI:16335`	small molecule
MET6 Scer `SGD:S000000893`	protein
SAM1 Scer `SGD:S000004170`	protein
S-adenosyl-L-homocysteine zwitterion `CHEBI:57856`	small molecule
water `CHEBI:15377`	small molecule
hydrogenphosphate `CHEBI:43474`	small molecule
diphosphoric acid `CHEBI:29888`	small molecule
L-homocysteine zwitterion `CHEBI:58199`	small molecule
R-H `CHEBI:142491`	small molecule
hydron `CHEBI:15378`	small molecule
a methylated methyl donor `gomodel:ba4cc2f6-35b0-45f1-a3b5-7bf7a1e71bf8_RXN-7605`	participant
5-methyltetrahydropteroyltri-L-glutamate(4-) `CHEBI:58207`	small molecule
SAH1 Scer `SGD:S000000845`	protein
SAM2 Scer `SGD:S000002910`	protein
tetrahydropteroyltri-L-glutamate(4-) `CHEBI:58140`	small molecule
zinc(2+) `CHEBI:29105`	cofactor
magnesium(2+) `CHEBI:18420`	cofactor
potassium(1+) `CHEBI:29103`	cofactor
NAD(1-) `CHEBI:57540`	cofactor
adenosylhomocysteinase activity `gomodel:ADENOSYLHOMOCYSTEINASE-RXN`	molecular activity
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity `gomodel:HOMOCYSMET-RXN`	molecular activity
S-methyltransferase activity `gomodel:RXN-7605`	molecular activity
methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	molecular activity
SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	has_output	S-adenosyl-L-methionine zwitterion `CHEBI:59789`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:S-ADENMETSYN-RXN RO:0002234 gomodel:CHEBI_59789_S-ADENMETSYN-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/0; endpoint types: /individuals/17/type; /individuals/14/type`
SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:YeastPathways_PWY-5041/6a2b236300005409 BFO:0000066 gomodel:YeastPathways_PWY-5041/6a2b236300005416. Source location `./YeastPathways_PWY-5041.json#/facts/1; endpoint types: /individuals/53/type; /individuals/59/type`
SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	has_output	S-adenosyl-L-methionine zwitterion `CHEBI:59789`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:YeastPathways_PWY-5041/6a2b236300005409 RO:0002234 gomodel:CHEBI_59789_S-ADENMETSYN-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/2; endpoint types: /individuals/53/type; /individuals/14/type`
methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	part_of	L-methionine cycle `GO:0033353`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:S-ADENMETSYN-RXN BFO:0000050 gomodel:YeastPathways_PWY-5041/YeastPathways_PWY-5041. Source location `./YeastPathways_PWY-5041.json#/facts/3; endpoint types: /individuals/17/type; /individuals/52/type`
SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	part_of	L-methionine cycle `GO:0033353`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:YeastPathways_PWY-5041/6a2b236300005409 BFO:0000050 gomodel:YeastPathways_PWY-5041/YeastPathways_PWY-5041. Source location `./YeastPathways_PWY-5041.json#/facts/4; endpoint types: /individuals/53/type; /individuals/52/type`
SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	has_input	L-methionine zwitterion `CHEBI:57844`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:YeastPathways_PWY-5041/6a2b236300005409 RO:0002233 gomodel:CHEBI_57844_HOMOCYSMET-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/5; endpoint types: /individuals/53/type; /individuals/9/type`
methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:S-ADENMETSYN-RXN RO:0002233 gomodel:CHEBI_30616_S-ADENMETSYN-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/6; endpoint types: /individuals/17/type; /individuals/7/type`
adenosylhomocysteinase activity `gomodel:ADENOSYLHOMOCYSTEINASE-RXN`	has_output	adenosine `CHEBI:16335`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:ADENOSYLHOMOCYSTEINASE-RXN RO:0002234 gomodel:CHEBI_16335_ADENOSYLHOMOCYSTEINASE-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/7; endpoint types: /individuals/0/type; /individuals/5/type`
MET6 Scer `SGD:S000000893`	enables	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity `gomodel:HOMOCYSMET-RXN`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:HOMOCYSMET-RXN RO:0002333 gomodel:YER091C-MONOMER_HOMOCYSMET-RXN_controller. Source location `./YeastPathways_PWY-5041.json#/facts/8; endpoint types: /individuals/15/type; /individuals/19/type`
SAM1 Scer `SGD:S000004170`	enables	methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:S-ADENMETSYN-RXN RO:0002333 gomodel:YLR180W-MONOMER_S-ADENMETSYN-RXN_controller. Source location `./YeastPathways_PWY-5041.json#/facts/9; endpoint types: /individuals/17/type; /individuals/20/type`
S-methyltransferase activity `gomodel:RXN-7605`	has_output	S-adenosyl-L-homocysteine zwitterion `CHEBI:57856`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:RXN-7605 RO:0002234 gomodel:CHEBI_57856_RXN-7605. Source location `./YeastPathways_PWY-5041.json#/facts/10; endpoint types: /individuals/16/type; /individuals/10/type`
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity `gomodel:HOMOCYSMET-RXN`	part_of	L-methionine cycle `GO:0033353`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:HOMOCYSMET-RXN BFO:0000050 gomodel:YeastPathways_PWY-5041/YeastPathways_PWY-5041. Source location `./YeastPathways_PWY-5041.json#/facts/11; endpoint types: /individuals/15/type; /individuals/52/type`
methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:S-ADENMETSYN-RXN RO:0002233 gomodel:CHEBI_15377_S-ADENMETSYN-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/12; endpoint types: /individuals/17/type; /individuals/3/type`
SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	has_output	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:YeastPathways_PWY-5041/6a2b236300005409 RO:0002234 gomodel:YeastPathways_PWY-5041/6a2b236300005413. Source location `./YeastPathways_PWY-5041.json#/facts/13; endpoint types: /individuals/53/type; /individuals/57/type`
SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:YeastPathways_PWY-5041/6a2b236300005409 RO:0002234 gomodel:YeastPathways_PWY-5041/6a2b236300005412. Source location `./YeastPathways_PWY-5041.json#/facts/14; endpoint types: /individuals/53/type; /individuals/56/type`
methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:S-ADENMETSYN-RXN BFO:0000066 gomodel:reaction_S-ADENMETSYN-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5041.json#/facts/15; endpoint types: /individuals/17/type; /individuals/51/type`
methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:S-ADENMETSYN-RXN RO:0002234 gomodel:CHEBI_29888_S-ADENMETSYN-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/16; endpoint types: /individuals/17/type; /individuals/6/type`
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity `gomodel:HOMOCYSMET-RXN`	has_input	L-homocysteine zwitterion `CHEBI:58199`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:HOMOCYSMET-RXN RO:0002233 gomodel:CHEBI_58199_ADENOSYLHOMOCYSTEINASE-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/17; endpoint types: /individuals/15/type; /individuals/12/type`
S-methyltransferase activity `gomodel:RXN-7605`	has_input	R-H `CHEBI:142491`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:RXN-7605 RO:0002233 gomodel:CHEBI_142491_RXN-7605. Source location `./YeastPathways_PWY-5041.json#/facts/18; endpoint types: /individuals/16/type; /individuals/1/type`
S-methyltransferase activity `gomodel:RXN-7605`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:RXN-7605 RO:0002234 gomodel:CHEBI_15378_RXN-7605. Source location `./YeastPathways_PWY-5041.json#/facts/19; endpoint types: /individuals/16/type; /individuals/4/type`
S-methyltransferase activity `gomodel:RXN-7605`	part_of	L-methionine cycle `GO:0033353`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:RXN-7605 BFO:0000050 gomodel:YeastPathways_PWY-5041/YeastPathways_PWY-5041. Source location `./YeastPathways_PWY-5041.json#/facts/20; endpoint types: /individuals/16/type; /individuals/52/type`
S-methyltransferase activity `gomodel:RXN-7605`	has_output	a methylated methyl donor `gomodel:ba4cc2f6-35b0-45f1-a3b5-7bf7a1e71bf8_RXN-7605`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:RXN-7605 RO:0002234 gomodel:ba4cc2f6-35b0-45f1-a3b5-7bf7a1e71bf8_RXN-7605. Source location `./YeastPathways_PWY-5041.json#/facts/21; endpoint types: /individuals/16/type; /individuals/21/type`
methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	has_input	L-methionine zwitterion `CHEBI:57844`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:S-ADENMETSYN-RXN RO:0002233 gomodel:CHEBI_57844_HOMOCYSMET-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/22; endpoint types: /individuals/17/type; /individuals/9/type`
adenosylhomocysteinase activity `gomodel:ADENOSYLHOMOCYSTEINASE-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:ADENOSYLHOMOCYSTEINASE-RXN BFO:0000066 gomodel:reaction_ADENOSYLHOMOCYSTEINASE-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5041.json#/facts/23; endpoint types: /individuals/0/type; /individuals/48/type`
S-methyltransferase activity `gomodel:RXN-7605`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:RXN-7605 BFO:0000066 gomodel:reaction_RXN-7605_location_lociGO_0005829. Source location `./YeastPathways_PWY-5041.json#/facts/24; endpoint types: /individuals/16/type; /individuals/50/type`
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity `gomodel:HOMOCYSMET-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:HOMOCYSMET-RXN BFO:0000066 gomodel:reaction_HOMOCYSMET-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5041.json#/facts/25; endpoint types: /individuals/15/type; /individuals/49/type`
SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:YeastPathways_PWY-5041/6a2b236300005409 RO:0002233 gomodel:YeastPathways_PWY-5041/6a2b236300005411. Source location `./YeastPathways_PWY-5041.json#/facts/26; endpoint types: /individuals/53/type; /individuals/55/type`
SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:YeastPathways_PWY-5041/6a2b236300005409 RO:0002233 gomodel:YeastPathways_PWY-5041/6a2b236300005410. Source location `./YeastPathways_PWY-5041.json#/facts/27; endpoint types: /individuals/53/type; /individuals/54/type`
adenosylhomocysteinase activity `gomodel:ADENOSYLHOMOCYSTEINASE-RXN`	has_input	S-adenosyl-L-homocysteine zwitterion `CHEBI:57856`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:ADENOSYLHOMOCYSTEINASE-RXN RO:0002233 gomodel:CHEBI_57856_RXN-7605. Source location `./YeastPathways_PWY-5041.json#/facts/28; endpoint types: /individuals/0/type; /individuals/10/type`
S-methyltransferase activity `gomodel:RXN-7605`	has_input	S-adenosyl-L-methionine zwitterion `CHEBI:59789`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:RXN-7605 RO:0002233 gomodel:CHEBI_59789_S-ADENMETSYN-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/29; endpoint types: /individuals/16/type; /individuals/14/type`
adenosylhomocysteinase activity `gomodel:ADENOSYLHOMOCYSTEINASE-RXN`	has_output	L-homocysteine zwitterion `CHEBI:58199`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:ADENOSYLHOMOCYSTEINASE-RXN RO:0002234 gomodel:CHEBI_58199_ADENOSYLHOMOCYSTEINASE-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/30; endpoint types: /individuals/0/type; /individuals/12/type`
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity `gomodel:HOMOCYSMET-RXN`	has_output	L-methionine zwitterion `CHEBI:57844`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:HOMOCYSMET-RXN RO:0002234 gomodel:CHEBI_57844_HOMOCYSMET-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/31; endpoint types: /individuals/15/type; /individuals/9/type`
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity `gomodel:HOMOCYSMET-RXN`	has_input	5-methyltetrahydropteroyltri-L-glutamate(4-) `CHEBI:58207`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:HOMOCYSMET-RXN RO:0002233 gomodel:CHEBI_58207_HOMOCYSMET-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/32; endpoint types: /individuals/15/type; /individuals/13/type`
SAH1 Scer `SGD:S000000845`	enables	adenosylhomocysteinase activity `gomodel:ADENOSYLHOMOCYSTEINASE-RXN`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:ADENOSYLHOMOCYSTEINASE-RXN RO:0002333 gomodel:YER043C-MONOMER_ADENOSYLHOMOCYSTEINASE-RXN_controller. Source location `./YeastPathways_PWY-5041.json#/facts/33; endpoint types: /individuals/0/type; /individuals/18/type`
adenosylhomocysteinase activity `gomodel:ADENOSYLHOMOCYSTEINASE-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:ADENOSYLHOMOCYSTEINASE-RXN RO:0002233 gomodel:CHEBI_15377_ADENOSYLHOMOCYSTEINASE-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/34; endpoint types: /individuals/0/type; /individuals/2/type`
SAM2 Scer `SGD:S000002910`	enables	SAM2 methionine adenosyltransferase activity `gomodel:YeastPathways_PWY-5041/6a2b236300005409`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:YeastPathways_PWY-5041/6a2b236300005409 RO:0002333 gomodel:YeastPathways_PWY-5041/6a2b236300005415. Source location `./YeastPathways_PWY-5041.json#/facts/35; endpoint types: /individuals/53/type; /individuals/58/type`
adenosylhomocysteinase activity `gomodel:ADENOSYLHOMOCYSTEINASE-RXN`	part_of	L-methionine cycle `GO:0033353`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:ADENOSYLHOMOCYSTEINASE-RXN BFO:0000050 gomodel:YeastPathways_PWY-5041/YeastPathways_PWY-5041. Source location `./YeastPathways_PWY-5041.json#/facts/36; endpoint types: /individuals/0/type; /individuals/52/type`
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity `gomodel:HOMOCYSMET-RXN`	has_output	tetrahydropteroyltri-L-glutamate(4-) `CHEBI:58140`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:HOMOCYSMET-RXN RO:0002234 gomodel:CHEBI_58140_HOMOCYSMET-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/37; endpoint types: /individuals/15/type; /individuals/11/type`
methionine adenosyltransferase activity `gomodel:S-ADENMETSYN-RXN`	has_output	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_PWY-5041 Source assertion: The model asserts gomodel:S-ADENMETSYN-RXN RO:0002234 gomodel:CHEBI_43474_S-ADENMETSYN-RXN. Source location `./YeastPathways_PWY-5041.json#/facts/38; endpoint types: /individuals/17/type; /individuals/8/type`
MET6 Scer `SGD:S000000893`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250	zinc(2+) `CHEBI:29105`	UniProtKB:P05694 Source assertion: UniProtKB:P05694 annotates CHEBI:29105 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3234/comments/0/cofactors/0` UniProtKB:P05694 Source assertion: UniProtKB:P05694 has the exact SGD cross-reference SGD:S000000893. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3234/uniProtKBCrossReferences/0`
SAM1 Scer `SGD:S000004170`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P13444) Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate. UniProt note evidence: ECO:0000250 (UniProtKB:P13444)	magnesium(2+) `CHEBI:18420`	UniProtKB:P10659 Source assertion: UniProtKB:P10659 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/310/comments/0/cofactors/0; /results/310/comments/0/note` UniProtKB:P10659 Source assertion: UniProtKB:P10659 has the exact SGD cross-reference SGD:S000004170. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/310/uniProtKBCrossReferences/0`
SAM1 Scer `SGD:S000004170`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P13444) Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate. UniProt note evidence: ECO:0000250 (UniProtKB:P13444)	potassium(1+) `CHEBI:29103`	UniProtKB:P10659 Source assertion: UniProtKB:P10659 annotates CHEBI:29103 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/310/comments/1/cofactors/0; /results/310/comments/1/note` UniProtKB:P10659 Source assertion: UniProtKB:P10659 has the exact SGD cross-reference SGD:S000004170. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/310/uniProtKBCrossReferences/0`
SAH1 Scer `SGD:S000000845`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified Binds 1 NAD(+) per subunit.	NAD(1-) `CHEBI:57540`	UniProtKB:P39954 Source assertion: UniProtKB:P39954 annotates CHEBI:57540 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3692/comments/0/cofactors/0; /results/3692/comments/0/note` UniProtKB:P39954 Source assertion: UniProtKB:P39954 has the exact SGD cross-reference SGD:S000000845. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3692/uniProtKBCrossReferences/0`
SAM2 Scer `SGD:S000002910`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P13444) Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate. UniProt note evidence: ECO:0000250 (UniProtKB:P13444)	magnesium(2+) `CHEBI:18420`	UniProtKB:P19358 Source assertion: UniProtKB:P19358 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/496/comments/0/cofactors/0; /results/496/comments/0/note` UniProtKB:P19358 Source assertion: UniProtKB:P19358 has the exact SGD cross-reference SGD:S000002910. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/496/uniProtKBCrossReferences/0`
SAM2 Scer `SGD:S000002910`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P13444) Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate. UniProt note evidence: ECO:0000250 (UniProtKB:P13444)	potassium(1+) `CHEBI:29103`	UniProtKB:P19358 Source assertion: UniProtKB:P19358 annotates CHEBI:29103 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/496/comments/1/cofactors/0; /results/496/comments/1/note` UniProtKB:P19358 Source assertion: UniProtKB:P19358 has the exact SGD cross-reference SGD:S000002910. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/496/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWY-5041 GO-CAM S-adenosyl-L-methionine cycle II model YeastPathways_PWY-5041 (source version: Pinned Noctua JSON archive; model date 2026-07-02; member ./YeastPathways_PWY-5041.json)
UniProtKB:P05694 UniProtKB P05694 cofactor annotations (source version: 2026_03)
UniProtKB:P10659 UniProtKB P10659 cofactor annotations (source version: 2026_03)
UniProtKB:P39954 UniProtKB P39954 cofactor annotations (source version: 2026_03)
UniProtKB:P19358 UniProtKB P19358 cofactor annotations (source version: 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- REVIEW — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 5 activity location assignments and 4 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 0 independently supported location edges and quarantined 0 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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