very long chain fatty acid biosynthesis I

gomodel:YeastPathways_PWY-5080-1

The very long chain fatty acid biosynthesis I GO-CAM for Saccharomyces cerevisiae S288C links ELO2/ELO3 fatty acid elongase, IFA38 acetoacetyl-CoA reductase, PHS1 enoyl-CoA hydratase, and TSC13 very-long-chain enoyl-CoA reductase activities that elongate long-chain fatty acyl-CoA through 3-oxo, 3-hydroxy, and trans-2-enoyl-CoA intermediates.

Pathway type

lipid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

26 nodes · 42 asserted directed edges

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Chemical / cofactor
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Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
carbon dioxide `CHEBI:16526`	small molecule
ELO2 Scer `SGD:S000000630`	protein
hydron `CHEBI:15378`	small molecule
cytosol `GO:0005829`	cellular component
TSC13 Scer `SGD:S000002173`	protein
trans-2-enoyl-CoA `CHEBI:50998`	lipid
3-oxo-fatty acyl-CoA `CHEBI:15489`	lipid
NADPH(4-) `CHEBI:57783`	small molecule
coenzyme A(4-) `CHEBI:57287`	small molecule
gomodel:46fed9f2-cf0b-4568-b40e-557bd0cfffe5_RXN3O-9813 `gomodel:46fed9f2-cf0b-4568-b40e-557bd0cfffe5_RXN3O-9813`	participant
very long-chain fatty acid biosynthetic process `GO:0042761`	biological process
long-chain fatty acyl-CoA `CHEBI:33184`	lipid
3-hydroxyacyl-CoA `CHEBI:65260`	small molecule
IFA38 Scer `SGD:S000000363`	protein
ELO3 Scer `SGD:S000004364`	protein
malonyl-CoA(5-) `CHEBI:57384`	small molecule
PHS1 Scer `SGD:S000003633`	protein
NADP(3-) `CHEBI:58349`	small molecule
water `CHEBI:15377`	small molecule
endoplasmic reticulum membrane `GO:0005789`	cellular component
vacuolar membrane `GO:0005774`	cellular component
a long-chain acyl-CoA + malonyl-CoA -> a 3-oxoacyl-CoA + CO₂ + coenzyme A `gomodel:RXN3O-9811`	molecular activity
a 3-oxoacyl-CoA + NADPH + H⁺ -> a 3-hydroxyacyl-CoA + NADP⁺ `gomodel:RXN3O-9812`	molecular activity
a 3-hydroxyacyl-CoA -> a trans-2-enoyl-CoA + H₂O `gomodel:RXN3O-9813`	molecular activity
a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	molecular activity
fatty acid elongase activity `gomodel:YeastPathways_PWY-5080-1/6a2b236300005423`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
a long-chain acyl-CoA + malonyl-CoA -> a 3-oxoacyl-CoA + CO₂ + coenzyme A `gomodel:RXN3O-9811`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9811 RO:0002234 gomodel:CHEBI_16526_RXN3O-9811. Source location `./YeastPathways_PWY-5080-1.json#/facts/0; endpoint types: /individuals/17/type; /individuals/4/type`
ELO2 Scer `SGD:S000000630`	enables	a long-chain acyl-CoA + malonyl-CoA -> a 3-oxoacyl-CoA + CO₂ + coenzyme A `gomodel:RXN3O-9811`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9811 RO:0002333 gomodel:YCR034W-MONOMER_RXN3O-9811_controller. Source location `./YeastPathways_PWY-5080-1.json#/facts/1; endpoint types: /individuals/17/type; /individuals/21/type`
a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9814 RO:0002233 gomodel:CHEBI_15378_RXN3O-9814. Source location `./YeastPathways_PWY-5080-1.json#/facts/2; endpoint types: /individuals/20/type; /individuals/2/type`
TSC13 Scer `SGD:S000002173`	enables	a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9814 RO:0002333 gomodel:MONOMER3O-101_RXN3O-9814_controller. Source location `./YeastPathways_PWY-5080-1.json#/facts/4; endpoint types: /individuals/20/type; /individuals/14/type`
a 3-hydroxyacyl-CoA -> a trans-2-enoyl-CoA + H₂O `gomodel:RXN3O-9813`	causally_upstream_of	a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9813 RO:0002411 gomodel:RXN3O-9814. Source location `./YeastPathways_PWY-5080-1.json#/facts/5; endpoint types: /individuals/19/type; /individuals/20/type`
a 3-hydroxyacyl-CoA -> a trans-2-enoyl-CoA + H₂O `gomodel:RXN3O-9813`	has_output	trans-2-enoyl-CoA `CHEBI:50998`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9813 RO:0002234 gomodel:a5c2c59b-7e94-48d3-ba08-123722f9430b_RXN3O-9813. Source location `./YeastPathways_PWY-5080-1.json#/facts/6; endpoint types: /individuals/19/type; /individuals/22/type`
a 3-oxoacyl-CoA + NADPH + H⁺ -> a 3-hydroxyacyl-CoA + NADP⁺ `gomodel:RXN3O-9812`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9812 RO:0002233 gomodel:CHEBI_15378_RXN3O-9812. Source location `./YeastPathways_PWY-5080-1.json#/facts/7; endpoint types: /individuals/18/type; /individuals/1/type`
a long-chain acyl-CoA + malonyl-CoA -> a 3-oxoacyl-CoA + CO₂ + coenzyme A `gomodel:RXN3O-9811`	has_output	3-oxo-fatty acyl-CoA `CHEBI:15489`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9811 RO:0002234 gomodel:CHEBI_15489_RXN3O-9811. Source location `./YeastPathways_PWY-5080-1.json#/facts/8; endpoint types: /individuals/17/type; /individuals/3/type`
a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9814 RO:0002233 gomodel:CHEBI_57783_RXN3O-9814. Source location `./YeastPathways_PWY-5080-1.json#/facts/9; endpoint types: /individuals/20/type; /individuals/10/type`
a long-chain acyl-CoA + malonyl-CoA -> a 3-oxoacyl-CoA + CO₂ + coenzyme A `gomodel:RXN3O-9811`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9811 RO:0002234 gomodel:CHEBI_57287_RXN3O-9811. Source location `./YeastPathways_PWY-5080-1.json#/facts/10; endpoint types: /individuals/17/type; /individuals/7/type`
a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	has_input	`gomodel:46fed9f2-cf0b-4568-b40e-557bd0cfffe5_RXN3O-9813`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9814 RO:0002233 gomodel:46fed9f2-cf0b-4568-b40e-557bd0cfffe5_RXN3O-9813. Source location `./YeastPathways_PWY-5080-1.json#/facts/12; endpoint types: /individuals/20/type; /individuals/54/type`
a 3-hydroxyacyl-CoA -> a trans-2-enoyl-CoA + H₂O `gomodel:RXN3O-9813`	part_of	very long-chain fatty acid biosynthetic process `GO:0042761`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9813 BFO:0000050 gomodel:YeastPathways_PWY-5080-1/YeastPathways_PWY-5080-1. Source location `./YeastPathways_PWY-5080-1.json#/facts/13; endpoint types: /individuals/19/type; /individuals/55/type`
a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	has_output	long-chain fatty acyl-CoA `CHEBI:33184`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9814 RO:0002234 gomodel:CHEBI_33184_RXN3O-9814. Source location `./YeastPathways_PWY-5080-1.json#/facts/14; endpoint types: /individuals/20/type; /individuals/6/type`
a 3-hydroxyacyl-CoA -> a trans-2-enoyl-CoA + H₂O `gomodel:RXN3O-9813`	has_input	3-hydroxyacyl-CoA `CHEBI:65260`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9813 RO:0002233 gomodel:CHEBI_65260_RXN3O-9812. Source location `./YeastPathways_PWY-5080-1.json#/facts/15; endpoint types: /individuals/19/type; /individuals/13/type`
IFA38 Scer `SGD:S000000363`	enables	a 3-oxoacyl-CoA + NADPH + H⁺ -> a 3-hydroxyacyl-CoA + NADP⁺ `gomodel:RXN3O-9812`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9812 RO:0002333 gomodel:MONOMER3O-79_RXN3O-9812_controller. Source location `./YeastPathways_PWY-5080-1.json#/facts/16; endpoint types: /individuals/18/type; /individuals/15/type`
ELO3 Scer `SGD:S000004364`	enables	fatty acid elongase activity `gomodel:YeastPathways_PWY-5080-1/6a2b236300005423`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:YeastPathways_PWY-5080-1/6a2b236300005423 RO:0002333 gomodel:YeastPathways_PWY-5080-1/6a2b236300005429. Source location `./YeastPathways_PWY-5080-1.json#/facts/17; endpoint types: /individuals/56/type; /individuals/61/type`
fatty acid elongase activity `gomodel:YeastPathways_PWY-5080-1/6a2b236300005423`	part_of	very long-chain fatty acid biosynthetic process `GO:0042761`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:YeastPathways_PWY-5080-1/6a2b236300005423 BFO:0000050 gomodel:YeastPathways_PWY-5080-1/YeastPathways_PWY-5080-1. Source location `./YeastPathways_PWY-5080-1.json#/facts/18; endpoint types: /individuals/56/type; /individuals/55/type`
a long-chain acyl-CoA + malonyl-CoA -> a 3-oxoacyl-CoA + CO₂ + coenzyme A `gomodel:RXN3O-9811`	has_input	long-chain fatty acyl-CoA `CHEBI:33184`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9811 RO:0002233 gomodel:CHEBI_33184_RXN3O-9811. Source location `./YeastPathways_PWY-5080-1.json#/facts/20; endpoint types: /individuals/17/type; /individuals/5/type`
a 3-oxoacyl-CoA + NADPH + H⁺ -> a 3-hydroxyacyl-CoA + NADP⁺ `gomodel:RXN3O-9812`	has_input	3-oxo-fatty acyl-CoA `CHEBI:15489`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9812 RO:0002233 gomodel:CHEBI_15489_RXN3O-9811. Source location `./YeastPathways_PWY-5080-1.json#/facts/21; endpoint types: /individuals/18/type; /individuals/3/type`
a long-chain acyl-CoA + malonyl-CoA -> a 3-oxoacyl-CoA + CO₂ + coenzyme A `gomodel:RXN3O-9811`	has_input	malonyl-CoA(5-) `CHEBI:57384`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9811 RO:0002233 gomodel:CHEBI_57384_RXN3O-9811. Source location `./YeastPathways_PWY-5080-1.json#/facts/22; endpoint types: /individuals/17/type; /individuals/8/type`
fatty acid elongase activity `gomodel:YeastPathways_PWY-5080-1/6a2b236300005423`	has_input	long-chain fatty acyl-CoA `CHEBI:33184`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:YeastPathways_PWY-5080-1/6a2b236300005423 RO:0002233 gomodel:YeastPathways_PWY-5080-1/6a2b236300005424. Source location `./YeastPathways_PWY-5080-1.json#/facts/23; endpoint types: /individuals/56/type; /individuals/57/type`
a long-chain acyl-CoA + malonyl-CoA -> a 3-oxoacyl-CoA + CO₂ + coenzyme A `gomodel:RXN3O-9811`	part_of	very long-chain fatty acid biosynthetic process `GO:0042761`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9811 BFO:0000050 gomodel:YeastPathways_PWY-5080-1/YeastPathways_PWY-5080-1. Source location `./YeastPathways_PWY-5080-1.json#/facts/24; endpoint types: /individuals/17/type; /individuals/55/type`
a 3-oxoacyl-CoA + NADPH + H⁺ -> a 3-hydroxyacyl-CoA + NADP⁺ `gomodel:RXN3O-9812`	has_output	3-hydroxyacyl-CoA `CHEBI:65260`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9812 RO:0002234 gomodel:CHEBI_65260_RXN3O-9812. Source location `./YeastPathways_PWY-5080-1.json#/facts/25; endpoint types: /individuals/18/type; /individuals/13/type`
fatty acid elongase activity `gomodel:YeastPathways_PWY-5080-1/6a2b236300005423`	has_input	malonyl-CoA(5-) `CHEBI:57384`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:YeastPathways_PWY-5080-1/6a2b236300005423 RO:0002233 gomodel:YeastPathways_PWY-5080-1/6a2b236300005425. Source location `./YeastPathways_PWY-5080-1.json#/facts/26; endpoint types: /individuals/56/type; /individuals/58/type`
PHS1 Scer `SGD:S000003633`	enables	a 3-hydroxyacyl-CoA -> a trans-2-enoyl-CoA + H₂O `gomodel:RXN3O-9813`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9813 RO:0002333 gomodel:MONOMER3O-85_RXN3O-9813_controller. Source location `./YeastPathways_PWY-5080-1.json#/facts/28; endpoint types: /individuals/19/type; /individuals/16/type`
a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	part_of	very long-chain fatty acid biosynthetic process `GO:0042761`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9814 BFO:0000050 gomodel:YeastPathways_PWY-5080-1/YeastPathways_PWY-5080-1. Source location `./YeastPathways_PWY-5080-1.json#/facts/30; endpoint types: /individuals/20/type; /individuals/55/type`
a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9814 RO:0002234 gomodel:CHEBI_58349_RXN3O-9814. Source location `./YeastPathways_PWY-5080-1.json#/facts/31; endpoint types: /individuals/20/type; /individuals/12/type`
fatty acid elongase activity `gomodel:YeastPathways_PWY-5080-1/6a2b236300005423`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:YeastPathways_PWY-5080-1/6a2b236300005423 RO:0002234 gomodel:YeastPathways_PWY-5080-1/6a2b236300005427. Source location `./YeastPathways_PWY-5080-1.json#/facts/32; endpoint types: /individuals/56/type; /individuals/60/type`
fatty acid elongase activity `gomodel:YeastPathways_PWY-5080-1/6a2b236300005423`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:YeastPathways_PWY-5080-1/6a2b236300005423 RO:0002234 gomodel:YeastPathways_PWY-5080-1/6a2b236300005426. Source location `./YeastPathways_PWY-5080-1.json#/facts/33; endpoint types: /individuals/56/type; /individuals/59/type`
a 3-oxoacyl-CoA + NADPH + H⁺ -> a 3-hydroxyacyl-CoA + NADP⁺ `gomodel:RXN3O-9812`	part_of	very long-chain fatty acid biosynthetic process `GO:0042761`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9812 BFO:0000050 gomodel:YeastPathways_PWY-5080-1/YeastPathways_PWY-5080-1. Source location `./YeastPathways_PWY-5080-1.json#/facts/34; endpoint types: /individuals/18/type; /individuals/55/type`
a 3-hydroxyacyl-CoA -> a trans-2-enoyl-CoA + H₂O `gomodel:RXN3O-9813`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9813 RO:0002234 gomodel:CHEBI_15377_RXN3O-9813. Source location `./YeastPathways_PWY-5080-1.json#/facts/35; endpoint types: /individuals/19/type; /individuals/0/type`
fatty acid elongase activity `gomodel:YeastPathways_PWY-5080-1/6a2b236300005423`	has_output	3-oxo-fatty acyl-CoA `CHEBI:15489`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:YeastPathways_PWY-5080-1/6a2b236300005423 RO:0002234 gomodel:CHEBI_15489_RXN3O-9811. Source location `./YeastPathways_PWY-5080-1.json#/facts/36; endpoint types: /individuals/56/type; /individuals/3/type`
a 3-oxoacyl-CoA + NADPH + H⁺ -> a 3-hydroxyacyl-CoA + NADP⁺ `gomodel:RXN3O-9812`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9812 RO:0002233 gomodel:CHEBI_57783_RXN3O-9812. Source location `./YeastPathways_PWY-5080-1.json#/facts/37; endpoint types: /individuals/18/type; /individuals/9/type`
a 3-oxoacyl-CoA + NADPH + H⁺ -> a 3-hydroxyacyl-CoA + NADP⁺ `gomodel:RXN3O-9812`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-5080-1 Source assertion: The model asserts gomodel:RXN3O-9812 RO:0002234 gomodel:CHEBI_58349_RXN3O-9812. Source location `./YeastPathways_PWY-5080-1.json#/facts/38; endpoint types: /individuals/18/type; /individuals/11/type`
ELO2 Scer `SGD:S000000630`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:12087109); ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:9832547) Topology: Multi-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P25358 Source assertion: UniProtKB:P25358 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/681/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P25358 Source assertion: UniProtKB:P25358 has the exact SGD cross-reference SGD:S000000630. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/681/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
TSC13 Scer `SGD:S000002173`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:11113186); ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:15958487) Topology: Multi-pass membrane protein. Source qualification: Accumulates at nucleus-vacuole (NV) junctions. Sequestred to NV junctions by NVJ1. Accumulates in nuclear PMN bleps and vesicles during stationary phase and nitrogen starvation	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:Q99190 Source assertion: UniProtKB:Q99190 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3182/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q99190 Source assertion: UniProtKB:Q99190 has the exact SGD cross-reference SGD:S000002173. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3182/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
IFA38 Scer `SGD:S000000363`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03107); ECO:0000269 (PubMed:23263984) Topology: Single-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P38286 Source assertion: UniProtKB:P38286 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1350/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P38286 Source assertion: UniProtKB:P38286 has the exact SGD cross-reference SGD:S000000363. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1350/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ELO3 Scer `SGD:S000004364`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:12087109); ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:9832547) Topology: Multi-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P40319 Source assertion: UniProtKB:P40319 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1690/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P40319 Source assertion: UniProtKB:P40319 has the exact SGD cross-reference SGD:S000004364. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1690/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
PHS1 Scer `SGD:S000003633`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:18272525) Topology: Multi-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P40857 Source assertion: UniProtKB:P40857 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1776/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P40857 Source assertion: UniProtKB:P40857 has the exact SGD cross-reference SGD:S000003633. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1776/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
PHS1 Scer `SGD:S000003633`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095) Topology: Multi-pass membrane protein.	vacuolar membrane `GO:0005774`	UniProtKB:P40857 Source assertion: UniProtKB:P40857 annotates location Vacuole membrane (SL-0271). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1776/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P40857 Source assertion: UniProtKB:P40857 has the exact SGD cross-reference SGD:S000003633. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1776/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
a 3-hydroxyacyl-CoA -> a trans-2-enoyl-CoA + H₂O `gomodel:RXN3O-9813`	occurs_in Activity compartment supported by an inspected functional annotation. ECO:0000269 (PubMed:17719544); ECO:0000269 (PubMed:18272525); ECO:0000269 (PubMed:23416297). Alternative isoenzymes may function elsewhere.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P40857 Source assertion: UniProtKB:P40857 describes the corresponding catalytic function in the endoplasmic reticulum membrane; this projection applies to the activity enabled by SGD:S000003633. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1776/comments/0/texts/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
a trans-2-enoyl-CoA + NADPH + H⁺ -> a long-chain acyl-CoA + NADP⁺ `gomodel:RXN3O-9814`	occurs_in Activity compartment supported by an inspected functional annotation. ECO:0000269 (PubMed:11113186); ECO:0000269 (PubMed:15958487). Alternative isoenzymes may function elsewhere.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:Q99190 Source assertion: UniProtKB:Q99190 describes the corresponding catalytic function in the endoplasmic reticulum membrane; this projection applies to the activity enabled by SGD:S000002173. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3182/comments/0/texts/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`

References

gomodel:YeastPathways_PWY-5080-1 SGD GO-CAM very long chain fatty acid biosynthesis I - imported from: Saccharomyces Genome Database (source version: Pinned Noctua JSON archive; model date 2026-07-02; member ./YeastPathways_PWY-5080-1.json)
UniProtKB:P25358 Reviewed UniProtKB P25358 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q99190 Reviewed UniProtKB Q99190 location annotations (source version: UniProt release 2026_03)
UniProtKB:P38286 Reviewed UniProtKB P38286 location annotations (source version: UniProt release 2026_03)
UniProtKB:P40319 Reviewed UniProtKB P40319 location annotations (source version: UniProt release 2026_03)
UniProtKB:P40857 Reviewed UniProtKB P40857 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 5 activity location assignments and 5 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 8 independently supported location edges and quarantined 5 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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