sulfate activation (for sulfonation)

gomodel:YeastPathways_PWY-5340

Saccharomyces cerevisiae S288C route that adenylates sulfate to adenylyl sulfate and phosphorylates it to 3'-phosphoadenylyl sulfate for sulfonation.

Pathway type

metabolic

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

14 nodes · 18 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
cytosol `GO:0005829`	cellular component
5'-adenylyl sulfate(2-) `CHEBI:58243`	small molecule
MET14 Scer `SGD:S000001484`	protein
sulfate `CHEBI:16189`	small molecule
hydron `CHEBI:15378`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
MET3 Scer `SGD:S000003771`	protein
3'-phosphonato-5'-adenylyl sulfate(4-) `CHEBI:58339`	small molecule
3'-phosphoadenosine 5'-phosphosulfate metabolic process `GO:0050427`	biological process
diphosphoric acid `CHEBI:29888`	small molecule
ADP(3-) `CHEBI:456216`	small molecule
cytoplasm `GO:0005737`	cellular component
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	molecular activity
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN BFO:0000066 gomodel:reaction_ADENYLYLSULFKIN-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5340.json#/facts/0; endpoint types: /individuals/0/type; /individuals/27/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_output	5'-adenylyl sulfate(2-) `CHEBI:58243`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002234 gomodel:CHEBI_58243_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/1; endpoint types: /individuals/10/type; /individuals/8/type`
MET14 Scer `SGD:S000001484`	enables	adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002333 gomodel:YKL001C-MONOMER_ADENYLYLSULFKIN-RXN_controller. Source location `./YeastPathways_PWY-5340.json#/facts/2; endpoint types: /individuals/0/type; /individuals/12/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	provides_input_for	adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002413 gomodel:ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/3; endpoint types: /individuals/10/type; /individuals/0/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_input	sulfate `CHEBI:16189`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002233 gomodel:CHEBI_16189_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/4; endpoint types: /individuals/10/type; /individuals/3/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002234 gomodel:CHEBI_15378_ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/5; endpoint types: /individuals/0/type; /individuals/1/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002233 gomodel:CHEBI_30616_ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/6; endpoint types: /individuals/0/type; /individuals/5/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN BFO:0000066 gomodel:reaction_SULFATE-ADENYLYLTRANS-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5340.json#/facts/7; endpoint types: /individuals/10/type; /individuals/28/type`
MET3 Scer `SGD:S000003771`	enables	sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002333 gomodel:YJR010W-MONOMER_SULFATE-ADENYLYLTRANS-RXN_controller. Source location `./YeastPathways_PWY-5340.json#/facts/8; endpoint types: /individuals/10/type; /individuals/11/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002233 gomodel:CHEBI_15378_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/9; endpoint types: /individuals/10/type; /individuals/2/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_output	3'-phosphonato-5'-adenylyl sulfate(4-) `CHEBI:58339`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002234 gomodel:CHEBI_58339_ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/10; endpoint types: /individuals/0/type; /individuals/9/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	part_of	3'-phosphoadenosine 5'-phosphosulfate metabolic process `GO:0050427`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN BFO:0000050 gomodel:YeastPathways_PWY-5340/YeastPathways_PWY-5340. Source location `./YeastPathways_PWY-5340.json#/facts/11; endpoint types: /individuals/0/type; /individuals/29/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002233 gomodel:CHEBI_30616_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/12; endpoint types: /individuals/10/type; /individuals/6/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002234 gomodel:CHEBI_29888_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/13; endpoint types: /individuals/10/type; /individuals/4/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_input	5'-adenylyl sulfate(2-) `CHEBI:58243`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002233 gomodel:CHEBI_58243_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/14; endpoint types: /individuals/0/type; /individuals/8/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_output	ADP(3-) `CHEBI:456216`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002234 gomodel:CHEBI_456216_ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_PWY-5340.json#/facts/15; endpoint types: /individuals/0/type; /individuals/7/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	part_of	3'-phosphoadenosine 5'-phosphosulfate metabolic process `GO:0050427`	gomodel:YeastPathways_PWY-5340 Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN BFO:0000050 gomodel:YeastPathways_PWY-5340/YeastPathways_PWY-5340. Source location `./YeastPathways_PWY-5340.json#/facts/16; endpoint types: /individuals/10/type; /individuals/29/type`
MET3 Scer `SGD:S000003771`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03106); ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P08536 Source assertion: UniProtKB:P08536 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/211/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P08536 Source assertion: UniProtKB:P08536 has the exact SGD cross-reference SGD:S000003771. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/211/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`

References

gomodel:YeastPathways_PWY-5340 SGD GO-CAM sulfate activation (for sulfonation) - imported from: Saccharomyces Genome Database (source version: Pinned Noctua JSON archive; model date 2026-08-04; member ./YeastPathways_PWY-5340.json)
UniProtKB:P08536 Reviewed UniProtKB P08536 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 2 activity location assignments and 2 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 1 independently supported location edges and quarantined 0 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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