L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde

gomodel:YeastPathways_PWY-5651

L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde in Saccharomyces cerevisiae S288C oxidizes L-tryptophan to N-formyl-L-kynurenine, hydrolyzes N-formyl-L-kynurenine to L-kynurenine through BNA7 arylformamidase activity, hydroxylates L-kynurenine to 3-hydroxy-L-kynurenine, hydrolyzes 3-hydroxy-L-kynurenine to 3-hydroxyanthranilate, and oxidizes 3-hydroxyanthranilate to cis,cis-2-amino-3-(3-oxoprop-1-enyl)but-2-enedioate.

Pathway type

amino acid degradation

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

33 nodes · 47 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
formate `CHEBI:15740`	small molecule
water `CHEBI:15377`	small molecule
cytosol `GO:0005829`	cellular component
hydron `CHEBI:15378`	small molecule
3-hydroxy-L-kynurenine zwitterion `CHEBI:58125`	small molecule
L-tryptophan catabolic process `GO:0006569`	biological process
L-kynurenine zwitterion `CHEBI:57959`	small molecule
BNA1 Scer `SGD:S000003786`	protein
cis,cis-2-amino-3-(3-oxoprop-1-enyl)but-2-enedioate `CHEBI:994`	small molecule
BNA4 Scer `SGD:S000000194`	protein
L-tryptophan zwitterion `CHEBI:57912`	small molecule
dioxygen `CHEBI:15379`	small molecule
BNA2 Scer `SGD:S000003839`	protein
NADPH(4-) `CHEBI:57783`	small molecule
BNA7 Scer `SGD:S000002836`	protein
3-hydroxyanthranilate `CHEBI:36559`	small molecule
N-formyl-L-kynurenine zwitterion `CHEBI:58629`	small molecule
NADP(3-) `CHEBI:58349`	small molecule
BNA5 Scer `SGD:S000004221`	protein
L-alanine zwitterion `CHEBI:57972`	small molecule
cytoplasm `GO:0005737`	cellular component
nucleus `GO:0005634`	cellular component
mitochondrial outer membrane `GO:0005741`	cellular component
mitochondrion `GO:0005739`	cellular component
iron(2+) `CHEBI:29033`	cofactor
FAD(3-) `CHEBI:57692`	cofactor
heme `CHEBI:30413`	cofactor
pyridoxal 5'-phosphate(2-) `CHEBI:597326`	cofactor
3-hydroxyanthranilate 3,4-dioxygenase activity `gomodel:1.13.11.6-RXN`	molecular activity
kynureninase activity `gomodel:3-HYDROXY-KYNURENINASE-RXN`	molecular activity
kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	molecular activity
indoleamine 2,3-dioxygenase activity `gomodel:RXN-8665`	molecular activity
arylformamidase activity (BNA7) `gomodel:YeastPathways_PWY-5651/6a4c244800001147`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
arylformamidase activity (BNA7) `gomodel:YeastPathways_PWY-5651/6a4c244800001147`	has_output	formate `CHEBI:15740`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:YeastPathways_PWY-5651/6a4c244800001147 RO:0002234 gomodel:YeastPathways_PWY-5651/6a4c244800001149. Source location `./YeastPathways_PWY-5651.json#/facts/0; endpoint types: /individuals/70/type; /individuals/72/type`
arylformamidase activity (BNA7) `gomodel:YeastPathways_PWY-5651/6a4c244800001147`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:YeastPathways_PWY-5651/6a4c244800001147 RO:0002233 gomodel:YeastPathways_PWY-5651/6a4c244800001148. Source location `./YeastPathways_PWY-5651.json#/facts/1; endpoint types: /individuals/70/type; /individuals/71/type`
indoleamine 2,3-dioxygenase activity `gomodel:RXN-8665`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:RXN-8665 BFO:0000066 gomodel:reaction_RXN-8665_location_lociGO_0005829. Source location `./YeastPathways_PWY-5651.json#/facts/2; endpoint types: /individuals/22/type; /individuals/66/type`
3-hydroxyanthranilate 3,4-dioxygenase activity `gomodel:1.13.11.6-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:1.13.11.6-RXN RO:0002234 gomodel:CHEBI_15378_1.13.11.6-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/3; endpoint types: /individuals/67/type; /individuals/4/type`
kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	has_output	3-hydroxy-L-kynurenine zwitterion `CHEBI:58125`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:KYNURENINE-3-MONOOXYGENASE-RXN RO:0002234 gomodel:CHEBI_58125_KYNURENINE-3-MONOOXYGENASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/4; endpoint types: /individuals/21/type; /individuals/17/type`
kynureninase activity `gomodel:3-HYDROXY-KYNURENINASE-RXN`	part_of	L-tryptophan catabolic process `GO:0006569`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:3-HYDROXY-KYNURENINASE-RXN BFO:0000050 gomodel:YeastPathways_PWY-5651/YeastPathways_PWY-5651. Source location `./YeastPathways_PWY-5651.json#/facts/5; endpoint types: /individuals/68/type; /individuals/69/type`
kynureninase activity `gomodel:3-HYDROXY-KYNURENINASE-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:3-HYDROXY-KYNURENINASE-RXN BFO:0000066 gomodel:reaction_3-HYDROXY-KYNURENINASE-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5651.json#/facts/6; endpoint types: /individuals/68/type; /individuals/63/type`
kynureninase activity `gomodel:3-HYDROXY-KYNURENINASE-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:3-HYDROXY-KYNURENINASE-RXN RO:0002233 gomodel:CHEBI_15377_3-HYDROXY-KYNURENINASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/8; endpoint types: /individuals/68/type; /individuals/1/type`
arylformamidase activity (BNA7) `gomodel:YeastPathways_PWY-5651/6a4c244800001147`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:YeastPathways_PWY-5651/6a4c244800001147 RO:0002234 gomodel:YeastPathways_PWY-5651/6a4c244800001150. Source location `./YeastPathways_PWY-5651.json#/facts/9; endpoint types: /individuals/70/type; /individuals/73/type`
kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	part_of	L-tryptophan catabolic process `GO:0006569`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:KYNURENINE-3-MONOOXYGENASE-RXN BFO:0000050 gomodel:YeastPathways_PWY-5651/YeastPathways_PWY-5651. Source location `./YeastPathways_PWY-5651.json#/facts/10; endpoint types: /individuals/21/type; /individuals/69/type`
BNA1 Scer `SGD:S000003786`	enables	3-hydroxyanthranilate 3,4-dioxygenase activity `gomodel:1.13.11.6-RXN`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:1.13.11.6-RXN RO:0002333 gomodel:YJR025C-MONOMER_1.13.11.6-RXN_controller. Source location `./YeastPathways_PWY-5651.json#/facts/11; endpoint types: /individuals/67/type; /individuals/25/type`
3-hydroxyanthranilate 3,4-dioxygenase activity `gomodel:1.13.11.6-RXN`	has_output	cis,cis-2-amino-3-(3-oxoprop-1-enyl)but-2-enedioate `CHEBI:994`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:1.13.11.6-RXN RO:0002234 gomodel:CHEBI_994_1.13.11.6-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/12; endpoint types: /individuals/67/type; /individuals/20/type`
kynureninase activity `gomodel:3-HYDROXY-KYNURENINASE-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:3-HYDROXY-KYNURENINASE-RXN RO:0002234 gomodel:CHEBI_15378_3-HYDROXY-KYNURENINASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/13; endpoint types: /individuals/68/type; /individuals/5/type`
BNA4 Scer `SGD:S000000194`	enables	kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:KYNURENINE-3-MONOOXYGENASE-RXN RO:0002333 gomodel:YBL098W-MONOMER_KYNURENINE-3-MONOOXYGENASE-RXN_controller. Source location `./YeastPathways_PWY-5651.json#/facts/14; endpoint types: /individuals/21/type; /individuals/23/type`
kynureninase activity `gomodel:3-HYDROXY-KYNURENINASE-RXN`	has_input	3-hydroxy-L-kynurenine zwitterion `CHEBI:58125`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:3-HYDROXY-KYNURENINASE-RXN RO:0002233 gomodel:CHEBI_58125_KYNURENINE-3-MONOOXYGENASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/15; endpoint types: /individuals/68/type; /individuals/17/type`
indoleamine 2,3-dioxygenase activity `gomodel:RXN-8665`	has_input	L-tryptophan zwitterion `CHEBI:57912`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:RXN-8665 RO:0002233 gomodel:CHEBI_57912_RXN-8665. Source location `./YeastPathways_PWY-5651.json#/facts/16; endpoint types: /individuals/22/type; /individuals/14/type`
indoleamine 2,3-dioxygenase activity `gomodel:RXN-8665`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:RXN-8665 RO:0002233 gomodel:CHEBI_15379_RXN-8665. Source location `./YeastPathways_PWY-5651.json#/facts/18; endpoint types: /individuals/22/type; /individuals/10/type`
3-hydroxyanthranilate 3,4-dioxygenase activity `gomodel:1.13.11.6-RXN`	part_of	L-tryptophan catabolic process `GO:0006569`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:1.13.11.6-RXN BFO:0000050 gomodel:YeastPathways_PWY-5651/YeastPathways_PWY-5651. Source location `./YeastPathways_PWY-5651.json#/facts/19; endpoint types: /individuals/67/type; /individuals/69/type`
BNA2 Scer `SGD:S000003839`	enables	indoleamine 2,3-dioxygenase activity `gomodel:RXN-8665`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:RXN-8665 RO:0002333 gomodel:YJR078W-MONOMER_RXN-8665_controller. Source location `./YeastPathways_PWY-5651.json#/facts/20; endpoint types: /individuals/22/type; /individuals/26/type`
kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:KYNURENINE-3-MONOOXYGENASE-RXN RO:0002233 gomodel:CHEBI_57783_KYNURENINE-3-MONOOXYGENASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/21; endpoint types: /individuals/21/type; /individuals/13/type`
arylformamidase activity (BNA7) `gomodel:YeastPathways_PWY-5651/6a4c244800001147`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:YeastPathways_PWY-5651/6a4c244800001147 BFO:0000066 gomodel:YeastPathways_PWY-5651/6a4c244800001153. Source location `./YeastPathways_PWY-5651.json#/facts/22; endpoint types: /individuals/70/type; /individuals/75/type`
indoleamine 2,3-dioxygenase activity `gomodel:RXN-8665`	part_of	L-tryptophan catabolic process `GO:0006569`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:RXN-8665 BFO:0000050 gomodel:YeastPathways_PWY-5651/YeastPathways_PWY-5651. Source location `./YeastPathways_PWY-5651.json#/facts/24; endpoint types: /individuals/22/type; /individuals/69/type`
BNA7 Scer `SGD:S000002836`	enables PMID:18205391 experimentally rejects BNA3 as formylkynurenine formamidase and identifies BNA7. The independently represented BNA7 reaction is retained.	arylformamidase activity (BNA7) `gomodel:YeastPathways_PWY-5651/6a4c244800001147`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:YeastPathways_PWY-5651/6a4c244800001147 RO:0002333 gomodel:YeastPathways_PWY-5651/6a4c244800001152. Source location `./YeastPathways_PWY-5651.json#/facts/25; endpoint types: /individuals/70/type; /individuals/74/type` UniProtKB:Q04066 Source assertion: UniProt Q04066 assigns N-formyl-L-kynurenine hydrolysis to BNA7, with experimental support from PMID:18205391. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/4112/comments/0`
kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:KYNURENINE-3-MONOOXYGENASE-RXN RO:0002233 gomodel:CHEBI_15378_KYNURENINE-3-MONOOXYGENASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/26; endpoint types: /individuals/21/type; /individuals/7/type`
3-hydroxyanthranilate 3,4-dioxygenase activity `gomodel:1.13.11.6-RXN`	has_input	3-hydroxyanthranilate `CHEBI:36559`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:1.13.11.6-RXN RO:0002233 gomodel:CHEBI_36559_3-HYDROXY-KYNURENINASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/27; endpoint types: /individuals/67/type; /individuals/12/type`
kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:KYNURENINE-3-MONOOXYGENASE-RXN RO:0002233 gomodel:CHEBI_15379_KYNURENINE-3-MONOOXYGENASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/29; endpoint types: /individuals/21/type; /individuals/9/type`
kynureninase activity `gomodel:3-HYDROXY-KYNURENINASE-RXN`	has_output	3-hydroxyanthranilate `CHEBI:36559`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:3-HYDROXY-KYNURENINASE-RXN RO:0002234 gomodel:CHEBI_36559_3-HYDROXY-KYNURENINASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/30; endpoint types: /individuals/68/type; /individuals/12/type`
kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:KYNURENINE-3-MONOOXYGENASE-RXN RO:0002234 gomodel:CHEBI_58349_KYNURENINE-3-MONOOXYGENASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/34; endpoint types: /individuals/21/type; /individuals/18/type`
arylformamidase activity (BNA7) `gomodel:YeastPathways_PWY-5651/6a4c244800001147`	has_input	N-formyl-L-kynurenine zwitterion `CHEBI:58629`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:YeastPathways_PWY-5651/6a4c244800001147 RO:0002233 gomodel:CHEBI_58629_RXN-8665. Source location `./YeastPathways_PWY-5651.json#/facts/35; endpoint types: /individuals/70/type; /individuals/19/type`
BNA5 Scer `SGD:S000004221`	enables	kynureninase activity `gomodel:3-HYDROXY-KYNURENINASE-RXN`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:3-HYDROXY-KYNURENINASE-RXN RO:0002333 gomodel:YLR231C-MONOMER_3-HYDROXY-KYNURENINASE-RXN_controller. Source location `./YeastPathways_PWY-5651.json#/facts/36; endpoint types: /individuals/68/type; /individuals/27/type`
kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	has_input	L-kynurenine zwitterion `CHEBI:57959`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:KYNURENINE-3-MONOOXYGENASE-RXN RO:0002233 gomodel:CHEBI_57959_ARYLFORMAMIDASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/37; endpoint types: /individuals/21/type; /individuals/15/type`
3-hydroxyanthranilate 3,4-dioxygenase activity `gomodel:1.13.11.6-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:1.13.11.6-RXN BFO:0000066 gomodel:reaction_1.13.11.6-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5651.json#/facts/40; endpoint types: /individuals/67/type; /individuals/62/type`
kynureninase activity `gomodel:3-HYDROXY-KYNURENINASE-RXN`	has_output	L-alanine zwitterion `CHEBI:57972`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:3-HYDROXY-KYNURENINASE-RXN RO:0002234 gomodel:CHEBI_57972_3-HYDROXY-KYNURENINASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/41; endpoint types: /individuals/68/type; /individuals/16/type`
3-hydroxyanthranilate 3,4-dioxygenase activity `gomodel:1.13.11.6-RXN`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:1.13.11.6-RXN RO:0002233 gomodel:CHEBI_15379_1.13.11.6-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/42; endpoint types: /individuals/67/type; /individuals/8/type`
kynurenine 3-monooxygenase activity `gomodel:KYNURENINE-3-MONOOXYGENASE-RXN`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:KYNURENINE-3-MONOOXYGENASE-RXN RO:0002234 gomodel:CHEBI_15377_KYNURENINE-3-MONOOXYGENASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/43; endpoint types: /individuals/21/type; /individuals/3/type`
arylformamidase activity (BNA7) `gomodel:YeastPathways_PWY-5651/6a4c244800001147`	part_of	L-tryptophan catabolic process `GO:0006569`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:YeastPathways_PWY-5651/6a4c244800001147 BFO:0000050 gomodel:YeastPathways_PWY-5651/YeastPathways_PWY-5651. Source location `./YeastPathways_PWY-5651.json#/facts/44; endpoint types: /individuals/70/type; /individuals/69/type`
indoleamine 2,3-dioxygenase activity `gomodel:RXN-8665`	has_output	N-formyl-L-kynurenine zwitterion `CHEBI:58629`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:RXN-8665 RO:0002234 gomodel:CHEBI_58629_RXN-8665. Source location `./YeastPathways_PWY-5651.json#/facts/45; endpoint types: /individuals/22/type; /individuals/19/type`
arylformamidase activity (BNA7) `gomodel:YeastPathways_PWY-5651/6a4c244800001147`	has_output	L-kynurenine zwitterion `CHEBI:57959`	gomodel:YeastPathways_PWY-5651 Source assertion: The model asserts gomodel:YeastPathways_PWY-5651/6a4c244800001147 RO:0002234 gomodel:CHEBI_57959_ARYLFORMAMIDASE-RXN. Source location `./YeastPathways_PWY-5651.json#/facts/46; endpoint types: /individuals/70/type; /individuals/15/type`
BNA1 Scer `SGD:S000003786`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03019); ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P47096 Source assertion: UniProtKB:P47096 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1960/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P47096 Source assertion: UniProtKB:P47096 has the exact SGD cross-reference SGD:S000003786. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1960/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
BNA1 Scer `SGD:S000003786`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	nucleus `GO:0005634`	UniProtKB:P47096 Source assertion: UniProtKB:P47096 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1960/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P47096 Source assertion: UniProtKB:P47096 has the exact SGD cross-reference SGD:S000003786. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1960/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
BNA4 Scer `SGD:S000000194`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03018); ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:14576278); ECO:0000269 (PubMed:16407407)	mitochondrial outer membrane `GO:0005741`	UniProtKB:P38169 Source assertion: UniProtKB:P38169 annotates location Mitochondrion outer membrane (SL-0172). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1305/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P38169 Source assertion: UniProtKB:P38169 has the exact SGD cross-reference SGD:S000000194. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1305/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
BNA5 Scer `SGD:S000004221`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03017); ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:Q05979 Source assertion: UniProtKB:Q05979 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2700/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q05979 Source assertion: UniProtKB:Q05979 has the exact SGD cross-reference SGD:S000004221. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2700/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
BNA5 Scer `SGD:S000004221`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	nucleus `GO:0005634`	UniProtKB:Q05979 Source assertion: UniProtKB:Q05979 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2700/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q05979 Source assertion: UniProtKB:Q05979 has the exact SGD cross-reference SGD:S000004221. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2700/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
BNA1 Scer `SGD:S000003786`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	iron(2+) `CHEBI:29033`	UniProtKB:P47096 Source assertion: UniProtKB:P47096 annotates CHEBI:29033 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1960/comments/0/cofactors/0` UniProtKB:P47096 Source assertion: UniProtKB:P47096 has the exact SGD cross-reference SGD:S000003786. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1960/uniProtKBCrossReferences/0`
BNA4 Scer `SGD:S000000194`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:29429898)	FAD(3-) `CHEBI:57692`	UniProtKB:P38169 Source assertion: UniProtKB:P38169 annotates CHEBI:57692 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1305/comments/0/cofactors/0` UniProtKB:P38169 Source assertion: UniProtKB:P38169 has the exact SGD cross-reference SGD:S000000194. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1305/uniProtKBCrossReferences/0`
BNA2 Scer `SGD:S000003839`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 heme group per subunit. UniProt note evidence: ECO:0000250	heme `CHEBI:30413`	UniProtKB:P47125 Source assertion: UniProtKB:P47125 annotates CHEBI:30413 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3878/comments/0/cofactors/0; /results/3878/comments/0/note` UniProtKB:P47125 Source assertion: UniProtKB:P47125 has the exact SGD cross-reference SGD:S000003839. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3878/uniProtKBCrossReferences/0`
BNA5 Scer `SGD:S000004221`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000255 (HAMAP-Rule:MF_03017)	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:Q05979 Source assertion: UniProtKB:Q05979 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2700/comments/0/cofactors/0` UniProtKB:Q05979 Source assertion: UniProtKB:Q05979 has the exact SGD cross-reference SGD:S000004221. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2700/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWY-5651 GO-CAM L-tryptophan degradation model YeastPathways_PWY-5651 (source version: Pinned Noctua JSON archive; model date 2026-07-10; member ./YeastPathways_PWY-5651.json)
UniProtKB:P47096 Reviewed UniProtKB P47096 location annotations (source version: UniProt release 2026_03)
UniProtKB:P38169 Reviewed UniProtKB P38169 location annotations (source version: UniProt release 2026_03)
UniProtKB:P47039 Reviewed UniProtKB P47039 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q05979 Reviewed UniProtKB Q05979 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q04066 Reviewed UniProt Q04066: catalytic-function assertions (source version: 2026_03)
UniProtKB:P47125 UniProtKB P47125 cofactor annotations (source version: 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 6 activity location assignments and 6 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 7 independently supported location edges and quarantined 1 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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2026-10-05T06:58:22Z claude-code
- EDIT — Remove superseded BNA3 formamidase branch
  Inspected PMID18205391 abstract and current UniProt Q04066/P47039 structured assertions. Removed obsolete BNA3 branch and its isolated physical-protein context; retained independently represented BNA7 formamidase activity with supporting evidence. Full removed edges and pinned primary-source hashes are in reports/causal_graph_review/gocam-assignment-conflicts.json. Schema validation and idempotent dry-run passed.
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