NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde

gomodel:YeastPathways_PWY-5653

NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde in Saccharomyces cerevisiae S288C cyclizes 2-amino-3-carboxymuconate-6-semialdehyde to quinolinic acid, converts quinolinic acid to nicotinate D-ribonucleotide, adenylates nicotinate D-ribonucleotide to deamido-NAD through NMA1 or NMA2 nicotinate-nucleotide adenylyltransferase activity, and amidates deamido-NAD with glutamine to form NAD.

Pathway type

cofactor biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

29 nodes · 48 asserted directed edges

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Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
cis,cis-2-amino-3-(3-oxoprop-1-enyl)but-2-enedioate `CHEBI:994`	small molecule
NAD(1-) `CHEBI:57540`	small molecule
adenosine 5'-monophosphate(2-) `CHEBI:456215`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
hydron `CHEBI:15378`	small molecule
NAD+ biosynthetic process `GO:0009435`	biological process
water `CHEBI:15377`	small molecule
nicotinate D-ribonucleotide(2-) `CHEBI:57502`	small molecule
cytosol `GO:0005829`	cellular component
QNS1 Scer `SGD:S000001116`	protein
diphosphoric acid `CHEBI:29888`	small molecule
quinolinic acid `CHEBI:16675`	small molecule
L-glutamine zwitterion `CHEBI:58359`	small molecule
BNA6 Scer `SGD:S000001943`	protein
NMA2 Scer `SGD:S000003242`	protein
L-glutamate(1-) `CHEBI:29985`	small molecule
deamido-NAD(2-) `CHEBI:58437`	small molecule
5-O-phosphonato-alpha-D-ribofuranosyl diphosphate(5-) `CHEBI:58017`	small molecule
NMA1 Scer `SGD:S000004320`	protein
carbon dioxide `CHEBI:16526`	small molecule
cytoplasm `GO:0005737`	cellular component
nucleus `GO:0005634`	cellular component
cobalt(2+) `CHEBI:48828`	cofactor
nickel(2+) `CHEBI:49786`	cofactor
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	molecular activity
nicotinate-nucleotide adenylyltransferase activity `gomodel:NICONUCADENYLYLTRAN-RXN`	molecular activity
nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	molecular activity
2-amino-3-carboxymuconate-6-semialdehyde cyclization `gomodel:RXN-5721`	molecular activity
nicotinate-nucleotide adenylyltransferase activity (NMA2) `gomodel:YeastPathways_PWY-5653/6a4c244800000432`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
2-amino-3-carboxymuconate-6-semialdehyde cyclization `gomodel:RXN-5721`	has_input	cis,cis-2-amino-3-(3-oxoprop-1-enyl)but-2-enedioate `CHEBI:994`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:RXN-5721 RO:0002233 gomodel:CHEBI_994_RXN-5721. Source location `./YeastPathways_PWY-5653.json#/facts/0; endpoint types: /individuals/24/type; /individuals/19/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	has_output	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002234 gomodel:CHEBI_57540_NAD-SYNTH-GLN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/1; endpoint types: /individuals/21/type; /individuals/15/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	has_output	adenosine 5'-monophosphate(2-) `CHEBI:456215`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002234 gomodel:CHEBI_456215_NAD-SYNTH-GLN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/2; endpoint types: /individuals/21/type; /individuals/13/type`
nicotinate-nucleotide adenylyltransferase activity (NMA2) `gomodel:YeastPathways_PWY-5653/6a4c244800000432`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:YeastPathways_PWY-5653/6a4c244800000432 RO:0002233 gomodel:YeastPathways_PWY-5653/6a4c244800000434. Source location `./YeastPathways_PWY-5653.json#/facts/3; endpoint types: /individuals/62/type; /individuals/64/type`
nicotinate-nucleotide adenylyltransferase activity (NMA2) `gomodel:YeastPathways_PWY-5653/6a4c244800000432`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:YeastPathways_PWY-5653/6a4c244800000432 RO:0002233 gomodel:YeastPathways_PWY-5653/6a4c244800000433. Source location `./YeastPathways_PWY-5653.json#/facts/4; endpoint types: /individuals/62/type; /individuals/63/type`
nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	part_of	NAD+ biosynthetic process `GO:0009435`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:QUINOPRIBOTRANS-RXN BFO:0000050 gomodel:YeastPathways_PWY-5653/YeastPathways_PWY-5653. Source location `./YeastPathways_PWY-5653.json#/facts/5; endpoint types: /individuals/23/type; /individuals/61/type`
2-amino-3-carboxymuconate-6-semialdehyde cyclization `gomodel:RXN-5721`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:RXN-5721 RO:0002234 gomodel:CHEBI_15377_RXN-5721. Source location `./YeastPathways_PWY-5653.json#/facts/6; endpoint types: /individuals/24/type; /individuals/1/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002234 gomodel:CHEBI_15378_NAD-SYNTH-GLN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/7; endpoint types: /individuals/21/type; /individuals/2/type`
nicotinate-nucleotide adenylyltransferase activity `gomodel:NICONUCADENYLYLTRAN-RXN`	has_input	nicotinate D-ribonucleotide(2-) `CHEBI:57502`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NICONUCADENYLYLTRAN-RXN RO:0002233 gomodel:CHEBI_57502_QUINOPRIBOTRANS-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/8; endpoint types: /individuals/22/type; /individuals/14/type`
2-amino-3-carboxymuconate-6-semialdehyde cyclization `gomodel:RXN-5721`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:RXN-5721 BFO:0000066 gomodel:reaction_RXN-5721_location_lociGO_0005829. Source location `./YeastPathways_PWY-5653.json#/facts/9; endpoint types: /individuals/24/type; /individuals/60/type`
QNS1 Scer `SGD:S000001116`	enables	NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002333 gomodel:MONOMER3O-845_NAD-SYNTH-GLN-RXN_controller. Source location `./YeastPathways_PWY-5653.json#/facts/10; endpoint types: /individuals/21/type; /individuals/20/type`
nicotinate-nucleotide adenylyltransferase activity `gomodel:NICONUCADENYLYLTRAN-RXN`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NICONUCADENYLYLTRAN-RXN RO:0002234 gomodel:CHEBI_29888_NICONUCADENYLYLTRAN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/11; endpoint types: /individuals/22/type; /individuals/8/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002233 gomodel:CHEBI_30616_NAD-SYNTH-GLN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/12; endpoint types: /individuals/21/type; /individuals/11/type`
nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	has_input	quinolinic acid `CHEBI:16675`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:QUINOPRIBOTRANS-RXN RO:0002233 gomodel:CHEBI_16675_RXN-5721. Source location `./YeastPathways_PWY-5653.json#/facts/13; endpoint types: /individuals/23/type; /individuals/6/type`
nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:QUINOPRIBOTRANS-RXN BFO:0000066 gomodel:reaction_QUINOPRIBOTRANS-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5653.json#/facts/14; endpoint types: /individuals/23/type; /individuals/59/type`
nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	has_output	nicotinate D-ribonucleotide(2-) `CHEBI:57502`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:QUINOPRIBOTRANS-RXN RO:0002234 gomodel:CHEBI_57502_QUINOPRIBOTRANS-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/15; endpoint types: /individuals/23/type; /individuals/14/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002233 gomodel:CHEBI_15377_NAD-SYNTH-GLN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/16; endpoint types: /individuals/21/type; /individuals/0/type`
nicotinate-nucleotide adenylyltransferase activity `gomodel:NICONUCADENYLYLTRAN-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NICONUCADENYLYLTRAN-RXN RO:0002233 gomodel:CHEBI_15378_NICONUCADENYLYLTRAN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/17; endpoint types: /individuals/22/type; /individuals/3/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	has_input	L-glutamine zwitterion `CHEBI:58359`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002233 gomodel:CHEBI_58359_NAD-SYNTH-GLN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/18; endpoint types: /individuals/21/type; /individuals/17/type`
nicotinate-nucleotide adenylyltransferase activity (NMA2) `gomodel:YeastPathways_PWY-5653/6a4c244800000432`	part_of	NAD+ biosynthetic process `GO:0009435`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:YeastPathways_PWY-5653/6a4c244800000432 BFO:0000050 gomodel:YeastPathways_PWY-5653/YeastPathways_PWY-5653. Source location `./YeastPathways_PWY-5653.json#/facts/19; endpoint types: /individuals/62/type; /individuals/61/type`
2-amino-3-carboxymuconate-6-semialdehyde cyclization `gomodel:RXN-5721`	part_of	NAD+ biosynthetic process `GO:0009435`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:RXN-5721 BFO:0000050 gomodel:YeastPathways_PWY-5653/YeastPathways_PWY-5653. Source location `./YeastPathways_PWY-5653.json#/facts/20; endpoint types: /individuals/24/type; /individuals/61/type`
BNA6 Scer `SGD:S000001943`	enables	nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:QUINOPRIBOTRANS-RXN RO:0002333 gomodel:YFR047C-MONOMER_QUINOPRIBOTRANS-RXN_controller. Source location `./YeastPathways_PWY-5653.json#/facts/21; endpoint types: /individuals/23/type; /individuals/25/type`
nicotinate-nucleotide adenylyltransferase activity (NMA2) `gomodel:YeastPathways_PWY-5653/6a4c244800000432`	has_input	nicotinate D-ribonucleotide(2-) `CHEBI:57502`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:YeastPathways_PWY-5653/6a4c244800000432 RO:0002233 gomodel:CHEBI_57502_QUINOPRIBOTRANS-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/23; endpoint types: /individuals/62/type; /individuals/14/type`
NMA2 Scer `SGD:S000003242`	enables	nicotinate-nucleotide adenylyltransferase activity (NMA2) `gomodel:YeastPathways_PWY-5653/6a4c244800000432`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:YeastPathways_PWY-5653/6a4c244800000432 RO:0002333 gomodel:YeastPathways_PWY-5653/6a4c244800000436. Source location `./YeastPathways_PWY-5653.json#/facts/24; endpoint types: /individuals/62/type; /individuals/66/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	has_output	L-glutamate(1-) `CHEBI:29985`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002234 gomodel:CHEBI_29985_NAD-SYNTH-GLN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/25; endpoint types: /individuals/21/type; /individuals/10/type`
nicotinate-nucleotide adenylyltransferase activity `gomodel:NICONUCADENYLYLTRAN-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NICONUCADENYLYLTRAN-RXN BFO:0000066 gomodel:reaction_NICONUCADENYLYLTRAN-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5653.json#/facts/26; endpoint types: /individuals/22/type; /individuals/58/type`
nicotinate-nucleotide adenylyltransferase activity (NMA2) `gomodel:YeastPathways_PWY-5653/6a4c244800000432`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:YeastPathways_PWY-5653/6a4c244800000432 RO:0002234 gomodel:YeastPathways_PWY-5653/6a4c244800000435. Source location `./YeastPathways_PWY-5653.json#/facts/27; endpoint types: /individuals/62/type; /individuals/65/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002234 gomodel:CHEBI_29888_NAD-SYNTH-GLN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/28; endpoint types: /individuals/21/type; /individuals/7/type`
nicotinate-nucleotide adenylyltransferase activity `gomodel:NICONUCADENYLYLTRAN-RXN`	has_output	deamido-NAD(2-) `CHEBI:58437`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NICONUCADENYLYLTRAN-RXN RO:0002234 gomodel:CHEBI_58437_NICONUCADENYLYLTRAN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/29; endpoint types: /individuals/22/type; /individuals/18/type`
nicotinate-nucleotide adenylyltransferase activity `gomodel:NICONUCADENYLYLTRAN-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NICONUCADENYLYLTRAN-RXN RO:0002233 gomodel:CHEBI_30616_NICONUCADENYLYLTRAN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/30; endpoint types: /individuals/22/type; /individuals/12/type`
nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:QUINOPRIBOTRANS-RXN RO:0002233 gomodel:CHEBI_15378_QUINOPRIBOTRANS-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/31; endpoint types: /individuals/23/type; /individuals/4/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	part_of	NAD+ biosynthetic process `GO:0009435`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN BFO:0000050 gomodel:YeastPathways_PWY-5653/YeastPathways_PWY-5653. Source location `./YeastPathways_PWY-5653.json#/facts/32; endpoint types: /individuals/21/type; /individuals/61/type`
nicotinate-nucleotide adenylyltransferase activity `gomodel:NICONUCADENYLYLTRAN-RXN`	part_of	NAD+ biosynthetic process `GO:0009435`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NICONUCADENYLYLTRAN-RXN BFO:0000050 gomodel:YeastPathways_PWY-5653/YeastPathways_PWY-5653. Source location `./YeastPathways_PWY-5653.json#/facts/33; endpoint types: /individuals/22/type; /individuals/61/type`
nicotinate-nucleotide adenylyltransferase activity (NMA2) `gomodel:YeastPathways_PWY-5653/6a4c244800000432`	has_output	deamido-NAD(2-) `CHEBI:58437`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:YeastPathways_PWY-5653/6a4c244800000432 RO:0002234 gomodel:CHEBI_58437_NICONUCADENYLYLTRAN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/34; endpoint types: /individuals/62/type; /individuals/18/type`
nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	has_input	5-O-phosphonato-alpha-D-ribofuranosyl diphosphate(5-) `CHEBI:58017`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:QUINOPRIBOTRANS-RXN RO:0002233 gomodel:CHEBI_58017_QUINOPRIBOTRANS-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/35; endpoint types: /individuals/23/type; /individuals/16/type`
NMA1 Scer `SGD:S000004320`	enables	nicotinate-nucleotide adenylyltransferase activity `gomodel:NICONUCADENYLYLTRAN-RXN`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NICONUCADENYLYLTRAN-RXN RO:0002333 gomodel:YLR328W-MONOMER_NICONUCADENYLYLTRAN-RXN_controller. Source location `./YeastPathways_PWY-5653.json#/facts/36; endpoint types: /individuals/22/type; /individuals/26/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	has_input	deamido-NAD(2-) `CHEBI:58437`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN RO:0002233 gomodel:CHEBI_58437_NICONUCADENYLYLTRAN-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/37; endpoint types: /individuals/21/type; /individuals/18/type`
NAD+ synthase (glutamine-hydrolyzing) activity `gomodel:NAD-SYNTH-GLN-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:NAD-SYNTH-GLN-RXN BFO:0000066 gomodel:reaction_NAD-SYNTH-GLN-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY-5653.json#/facts/38; endpoint types: /individuals/21/type; /individuals/57/type`
nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:QUINOPRIBOTRANS-RXN RO:0002234 gomodel:CHEBI_16526_QUINOPRIBOTRANS-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/39; endpoint types: /individuals/23/type; /individuals/5/type`
2-amino-3-carboxymuconate-6-semialdehyde cyclization `gomodel:RXN-5721`	has_output	quinolinic acid `CHEBI:16675`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:RXN-5721 RO:0002234 gomodel:CHEBI_16675_RXN-5721. Source location `./YeastPathways_PWY-5653.json#/facts/40; endpoint types: /individuals/24/type; /individuals/6/type`
nicotinate-nucleotide diphosphorylase (carboxylating) activity `gomodel:QUINOPRIBOTRANS-RXN`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_PWY-5653 Source assertion: The model asserts gomodel:QUINOPRIBOTRANS-RXN RO:0002234 gomodel:CHEBI_29888_QUINOPRIBOTRANS-RXN. Source location `./YeastPathways_PWY-5653.json#/facts/41; endpoint types: /individuals/23/type; /individuals/9/type`
BNA6 Scer `SGD:S000001943`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P43619 Source assertion: UniProtKB:P43619 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3843/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P43619 Source assertion: UniProtKB:P43619 has the exact SGD cross-reference SGD:S000001943. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3843/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
BNA6 Scer `SGD:S000001943`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	nucleus `GO:0005634`	UniProtKB:P43619 Source assertion: UniProtKB:P43619 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3843/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P43619 Source assertion: UniProtKB:P43619 has the exact SGD cross-reference SGD:S000001943. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3843/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
NMA2 Scer `SGD:S000003242`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:11884393)	nucleus `GO:0005634`	UniProtKB:P53204 Source assertion: UniProtKB:P53204 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2159/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P53204 Source assertion: UniProtKB:P53204 has the exact SGD cross-reference SGD:S000003242. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2159/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
NMA1 Scer `SGD:S000004320`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:Q06178 Source assertion: UniProtKB:Q06178 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2726/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q06178 Source assertion: UniProtKB:Q06178 has the exact SGD cross-reference SGD:S000004320. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2726/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
NMA1 Scer `SGD:S000004320`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	nucleus `GO:0005634`	UniProtKB:Q06178 Source assertion: UniProtKB:Q06178 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2726/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q06178 Source assertion: UniProtKB:Q06178 has the exact SGD cross-reference SGD:S000004320. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2726/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
NMA2 Scer `SGD:S000003242`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:12597897) Divalent metal cation. UniProt note evidence: ECO:0000269 (PubMed:12597897)	cobalt(2+) `CHEBI:48828`	UniProtKB:P53204 Source assertion: UniProtKB:P53204 annotates CHEBI:48828 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2159/comments/0/cofactors/0; /results/2159/comments/0/note` UniProtKB:P53204 Source assertion: UniProtKB:P53204 has the exact SGD cross-reference SGD:S000003242. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2159/uniProtKBCrossReferences/0`
NMA1 Scer `SGD:S000004320`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:10428462) Divalent metal cation. UniProt note evidence: ECO:0000269 (PubMed:10428462)	nickel(2+) `CHEBI:49786`	UniProtKB:Q06178 Source assertion: UniProtKB:Q06178 annotates CHEBI:49786 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2726/comments/0/cofactors/0; /results/2726/comments/0/note` UniProtKB:Q06178 Source assertion: UniProtKB:Q06178 has the exact SGD cross-reference SGD:S000004320. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2726/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWY-5653 GO-CAM NAD biosynthesis model YeastPathways_PWY-5653 (source version: Pinned Noctua JSON archive; model date 2026-07-07; member ./YeastPathways_PWY-5653.json)
UniProtKB:P43619 Reviewed UniProtKB P43619 location annotations (source version: UniProt release 2026_03)
UniProtKB:P53204 Reviewed UniProtKB P53204 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q06178 Reviewed UniProtKB Q06178 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 5 activity location assignments and 4 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 5 independently supported location edges and quarantined 1 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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