zymosterol biosynthesis

gomodel:YeastPathways_PWY-6074-1

Zymosterol biosynthesis in Saccharomyces cerevisiae S288C converts lanosterol to zymosterol through ERG11, ERG24, ERG25, ERG26, and ERG27 sterol oxidation, reduction, and decarboxylation activities.

Pathway type

lipid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

46 nodes · 102 asserted directed edges

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
ERG25 Scer `SGD:S000003292`	protein
water `CHEBI:15377`	small molecule
zymosterol biosynthetic process `GO:0036197`	biological process
ERG11 Scer `SGD:S000001049`	protein
hydron `CHEBI:15378`	small molecule
NAD(P)H `CHEBI:13392`	small molecule
NADP(3-) `CHEBI:58349`	small molecule
cytosol `GO:0005829`	cellular component
NADPH(4-) `CHEBI:57783`	small molecule
4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol `CHEBI:17813`	lipid
zymosterone `CHEBI:52386`	lipid
3-dehydro-4alpha-methylzymosterol `CHEBI:136486`	lipid
NAD(P)(+) `CHEBI:13390`	small molecule
dioxygen `CHEBI:15379`	small molecule
formate `CHEBI:15740`	small molecule
4alpha-hydroxymethyl-4beta-methylzymosterol `CHEBI:87289`	lipid
ERG27 Scer `SGD:S000004090`	protein
4alpha-methylzymosterol `CHEBI:1949`	lipid
ERG24 Scer `SGD:S000005224`	protein
14-demethyllanosterol `CHEBI:18364`	lipid
4alpha-carboxyzymosterol(1-) `CHEBI:143575`	small molecule
4alpha-hydroxymethylzymosterol `CHEBI:146130`	lipid
4alpha-formylzymosterol `CHEBI:146131`	lipid
carbon dioxide `CHEBI:16526`	small molecule
ERG26 Scer `SGD:S000002969`	protein
4beta-methylzymosterol-4alpha-carboxylate `CHEBI:64925`	small molecule
4beta-methylzymosterol-4-carbaldehyde `CHEBI:87287`	lipid
lanosterol `CHEBI:16521`	lipid
zymosterol `CHEBI:18252`	lipid
endoplasmic reticulum membrane `GO:0005789`	cellular component
lipid droplet `GO:0005811`	cellular component
membrane `GO:0016020`	cellular component
iron cation `CHEBI:24875`	cofactor
heme `CHEBI:30413`	cofactor
sterol 14-demethylase activity `gomodel:RXN3O-9820`	molecular activity
C-4 methylsterol oxidase activity `gomodel:RXN3O-9821`	molecular activity
C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	molecular activity
C-4 methylsterol oxidase activity `gomodel:RXN3O-9823`	molecular activity
C-4 methylsterol oxidase activity `gomodel:RXN3O-9824`	molecular activity
C-4 methylsterol oxidase activity `gomodel:RXN3O-9825`	molecular activity
C-4 methylsterol oxidase activity `gomodel:RXN3O-9826`	molecular activity
Delta14-sterol reductase activity `gomodel:RXN66-306`	molecular activity
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-313`	molecular activity
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-314`	molecular activity
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-318`	molecular activity
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-319`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
ERG25 Scer `SGD:S000003292`	enables	C-4 methylsterol oxidase activity `gomodel:RXN3O-9826`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9826 RO:0002333 gomodel:YGR060W-MONOMER_RXN3O-9826_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/0; endpoint types: /individuals/62/type; /individuals/75/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9822 RO:0002234 gomodel:CHEBI_15377_RXN3O-9822. Source location `./YeastPathways_PWY-6074-1.json#/facts/1; endpoint types: /individuals/58/type; /individuals/20/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-314`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-314 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/2; endpoint types: /individuals/65/type; /individuals/186/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9823`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9823 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/3; endpoint types: /individuals/59/type; /individuals/186/type`
ERG11 Scer `SGD:S000001049`	enables	sterol 14-demethylase activity `gomodel:RXN3O-9820`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 RO:0002333 gomodel:YHR007C-MONOMER_RXN3O-9820_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/4; endpoint types: /individuals/56/type; /individuals/76/type`
sterol 14-demethylase activity `gomodel:RXN3O-9820`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 RO:0002233 gomodel:CHEBI_15378_RXN3O-9820. Source location `./YeastPathways_PWY-6074-1.json#/facts/5; endpoint types: /individuals/56/type; /individuals/25/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9823`	has_input	NAD(P)H `CHEBI:13392`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9823 RO:0002233 gomodel:CHEBI_13392_RXN3O-9823. Source location `./YeastPathways_PWY-6074-1.json#/facts/6; endpoint types: /individuals/59/type; /individuals/8/type`
Delta14-sterol reductase activity `gomodel:RXN66-306`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-306 RO:0002234 gomodel:CHEBI_58349_RXN66-306. Source location `./YeastPathways_PWY-6074-1.json#/facts/7; endpoint types: /individuals/63/type; /individuals/50/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-319`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-319 RO:0002233 gomodel:CHEBI_15378_RXN66-319. Source location `./YeastPathways_PWY-6074-1.json#/facts/9; endpoint types: /individuals/67/type; /individuals/28/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-319`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-319 RO:0002233 gomodel:CHEBI_57783_RXN66-319. Source location `./YeastPathways_PWY-6074-1.json#/facts/10; endpoint types: /individuals/67/type; /individuals/48/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-313`	has_output	NAD(P)H `CHEBI:13392`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-313 RO:0002234 gomodel:CHEBI_13392_RXN66-313. Source location `./YeastPathways_PWY-6074-1.json#/facts/11; endpoint types: /individuals/64/type; /individuals/12/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9825`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9825 RO:0002234 gomodel:CHEBI_15377_RXN3O-9825. Source location `./YeastPathways_PWY-6074-1.json#/facts/12; endpoint types: /individuals/61/type; /individuals/23/type`
sterol 14-demethylase activity `gomodel:RXN3O-9820`	has_output	4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol `CHEBI:17813`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 RO:0002234 gomodel:CHEBI_17813_RXN3O-9820. Source location `./YeastPathways_PWY-6074-1.json#/facts/13; endpoint types: /individuals/56/type; /individuals/40/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9822 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/15; endpoint types: /individuals/58/type; /individuals/186/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-319`	has_input	zymosterone `CHEBI:52386`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-319 RO:0002233 gomodel:CHEBI_52386_RXN66-318. Source location `./YeastPathways_PWY-6074-1.json#/facts/16; endpoint types: /individuals/67/type; /individuals/44/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-314`	has_input	3-dehydro-4alpha-methylzymosterol `CHEBI:136486`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-314 RO:0002233 gomodel:CHEBI_136486_RXN66-313. Source location `./YeastPathways_PWY-6074-1.json#/facts/18; endpoint types: /individuals/65/type; /individuals/14/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9826`	has_output	NAD(P)(+) `CHEBI:13390`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9826 RO:0002234 gomodel:CHEBI_13390_RXN3O-9826. Source location `./YeastPathways_PWY-6074-1.json#/facts/19; endpoint types: /individuals/62/type; /individuals/5/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9825`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9825 RO:0002233 gomodel:CHEBI_15379_RXN3O-9825. Source location `./YeastPathways_PWY-6074-1.json#/facts/20; endpoint types: /individuals/61/type; /individuals/34/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9821`	has_output	NAD(P)(+) `CHEBI:13390`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9821 RO:0002234 gomodel:CHEBI_13390_RXN3O-9821. Source location `./YeastPathways_PWY-6074-1.json#/facts/21; endpoint types: /individuals/57/type; /individuals/0/type`
sterol 14-demethylase activity `gomodel:RXN3O-9820`	has_output	formate `CHEBI:15740`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 RO:0002234 gomodel:CHEBI_15740_RXN3O-9820. Source location `./YeastPathways_PWY-6074-1.json#/facts/22; endpoint types: /individuals/56/type; /individuals/36/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-314`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-314 RO:0002233 gomodel:CHEBI_57783_RXN66-314. Source location `./YeastPathways_PWY-6074-1.json#/facts/23; endpoint types: /individuals/65/type; /individuals/47/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	has_input	4alpha-hydroxymethyl-4beta-methylzymosterol `CHEBI:87289`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9822 RO:0002233 gomodel:CHEBI_87289_RXN3O-9821. Source location `./YeastPathways_PWY-6074-1.json#/facts/24; endpoint types: /individuals/58/type; /individuals/55/type`
ERG25 Scer `SGD:S000003292`	enables	C-4 methylsterol oxidase activity `gomodel:RXN3O-9821`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9821 RO:0002333 gomodel:YGR060W-MONOMER_RXN3O-9821_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/26; endpoint types: /individuals/57/type; /individuals/70/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9821`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9821 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/27; endpoint types: /individuals/57/type; /individuals/186/type`
ERG27 Scer `SGD:S000004090`	enables	3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-314`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-314 RO:0002333 gomodel:YLR100W-MONOMER_RXN66-314_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/28; endpoint types: /individuals/65/type; /individuals/77/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9825`	has_input	NAD(P)H `CHEBI:13392`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9825 RO:0002233 gomodel:CHEBI_13392_RXN3O-9825. Source location `./YeastPathways_PWY-6074-1.json#/facts/29; endpoint types: /individuals/61/type; /individuals/10/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9824`	has_output	NAD(P)(+) `CHEBI:13390`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9824 RO:0002234 gomodel:CHEBI_13390_RXN3O-9824. Source location `./YeastPathways_PWY-6074-1.json#/facts/30; endpoint types: /individuals/60/type; /individuals/3/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9824`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9824 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/32; endpoint types: /individuals/60/type; /individuals/186/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-319`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-319 RO:0002234 gomodel:CHEBI_58349_RXN66-319. Source location `./YeastPathways_PWY-6074-1.json#/facts/33; endpoint types: /individuals/67/type; /individuals/52/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9824`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9824 RO:0002233 gomodel:CHEBI_15379_RXN3O-9824. Source location `./YeastPathways_PWY-6074-1.json#/facts/35; endpoint types: /individuals/60/type; /individuals/33/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-313`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-313 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/36; endpoint types: /individuals/64/type; /individuals/186/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9822 RO:0002233 gomodel:CHEBI_15379_RXN3O-9822. Source location `./YeastPathways_PWY-6074-1.json#/facts/37; endpoint types: /individuals/58/type; /individuals/31/type`
ERG27 Scer `SGD:S000004090`	enables	3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-319`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-319 RO:0002333 gomodel:YLR100W-MONOMER_RXN66-319_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/38; endpoint types: /individuals/67/type; /individuals/78/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9821`	has_output	4alpha-hydroxymethyl-4beta-methylzymosterol `CHEBI:87289`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9821 RO:0002234 gomodel:CHEBI_87289_RXN3O-9821. Source location `./YeastPathways_PWY-6074-1.json#/facts/39; endpoint types: /individuals/57/type; /individuals/55/type`
ERG25 Scer `SGD:S000003292`	enables	C-4 methylsterol oxidase activity `gomodel:RXN3O-9824`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9824 RO:0002333 gomodel:YGR060W-MONOMER_RXN3O-9824_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/40; endpoint types: /individuals/60/type; /individuals/73/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9821`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9821 RO:0002234 gomodel:CHEBI_15377_RXN3O-9821. Source location `./YeastPathways_PWY-6074-1.json#/facts/41; endpoint types: /individuals/57/type; /individuals/19/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9824`	has_input	4alpha-methylzymosterol `CHEBI:1949`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9824 RO:0002233 gomodel:CHEBI_1949_RXN66-314. Source location `./YeastPathways_PWY-6074-1.json#/facts/42; endpoint types: /individuals/60/type; /individuals/43/type`
ERG24 Scer `SGD:S000005224`	enables	Delta14-sterol reductase activity `gomodel:RXN66-306`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-306 RO:0002333 gomodel:YNL280C-MONOMER_RXN66-306_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/45; endpoint types: /individuals/63/type; /individuals/79/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9821`	has_input	14-demethyllanosterol `CHEBI:18364`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9821 RO:0002233 gomodel:CHEBI_18364_RXN66-306. Source location `./YeastPathways_PWY-6074-1.json#/facts/46; endpoint types: /individuals/57/type; /individuals/42/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-318`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-318 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/47; endpoint types: /individuals/66/type; /individuals/186/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9826`	has_output	4alpha-carboxyzymosterol(1-) `CHEBI:143575`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9826 RO:0002234 gomodel:CHEBI_143575_RXN3O-9826. Source location `./YeastPathways_PWY-6074-1.json#/facts/48; endpoint types: /individuals/62/type; /individuals/15/type`
Delta14-sterol reductase activity `gomodel:RXN66-306`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-306 RO:0002233 gomodel:CHEBI_57783_RXN66-306. Source location `./YeastPathways_PWY-6074-1.json#/facts/49; endpoint types: /individuals/63/type; /individuals/46/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9825`	has_input	4alpha-hydroxymethylzymosterol `CHEBI:146130`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9825 RO:0002233 gomodel:CHEBI_146130_RXN3O-9824. Source location `./YeastPathways_PWY-6074-1.json#/facts/50; endpoint types: /individuals/61/type; /individuals/16/type`
sterol 14-demethylase activity `gomodel:RXN3O-9820`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 RO:0002234 gomodel:CHEBI_58349_RXN3O-9820. Source location `./YeastPathways_PWY-6074-1.json#/facts/51; endpoint types: /individuals/56/type; /individuals/49/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9825`	has_output	4alpha-formylzymosterol `CHEBI:146131`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9825 RO:0002234 gomodel:CHEBI_146131_RXN3O-9825. Source location `./YeastPathways_PWY-6074-1.json#/facts/52; endpoint types: /individuals/61/type; /individuals/17/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9823`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9823 RO:0002233 gomodel:CHEBI_15379_RXN3O-9823. Source location `./YeastPathways_PWY-6074-1.json#/facts/53; endpoint types: /individuals/59/type; /individuals/32/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-318`	has_output	NAD(P)H `CHEBI:13392`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-318 RO:0002234 gomodel:CHEBI_13392_RXN66-318. Source location `./YeastPathways_PWY-6074-1.json#/facts/54; endpoint types: /individuals/66/type; /individuals/13/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9826`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9826 RO:0002234 gomodel:CHEBI_15377_RXN3O-9826. Source location `./YeastPathways_PWY-6074-1.json#/facts/56; endpoint types: /individuals/62/type; /individuals/24/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	has_output	NAD(P)(+) `CHEBI:13390`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9822 RO:0002234 gomodel:CHEBI_13390_RXN3O-9822. Source location `./YeastPathways_PWY-6074-1.json#/facts/57; endpoint types: /individuals/58/type; /individuals/1/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9826`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9826 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/58; endpoint types: /individuals/62/type; /individuals/186/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-318`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-318 RO:0002234 gomodel:CHEBI_16526_RXN66-318. Source location `./YeastPathways_PWY-6074-1.json#/facts/59; endpoint types: /individuals/66/type; /individuals/39/type`
ERG26 Scer `SGD:S000002969`	enables	3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-313`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-313 RO:0002333 gomodel:YGL001C-MONOMER_RXN66-313_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/61; endpoint types: /individuals/64/type; /individuals/68/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9823`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9823 RO:0002234 gomodel:CHEBI_15377_RXN3O-9823. Source location `./YeastPathways_PWY-6074-1.json#/facts/62; endpoint types: /individuals/59/type; /individuals/21/type`
sterol 14-demethylase activity `gomodel:RXN3O-9820`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 RO:0002233 gomodel:CHEBI_57783_RXN3O-9820. Source location `./YeastPathways_PWY-6074-1.json#/facts/63; endpoint types: /individuals/56/type; /individuals/45/type`
sterol 14-demethylase activity `gomodel:RXN3O-9820`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 RO:0002233 gomodel:CHEBI_15379_RXN3O-9820. Source location `./YeastPathways_PWY-6074-1.json#/facts/64; endpoint types: /individuals/56/type; /individuals/29/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9826`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9826 RO:0002233 gomodel:CHEBI_15379_RXN3O-9826. Source location `./YeastPathways_PWY-6074-1.json#/facts/65; endpoint types: /individuals/62/type; /individuals/35/type`
ERG25 Scer `SGD:S000003292`	enables	C-4 methylsterol oxidase activity `gomodel:RXN3O-9823`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9823 RO:0002333 gomodel:YGR060W-MONOMER_RXN3O-9823_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/66; endpoint types: /individuals/59/type; /individuals/72/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	has_input	NAD(P)H `CHEBI:13392`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9822 RO:0002233 gomodel:CHEBI_13392_RXN3O-9822. Source location `./YeastPathways_PWY-6074-1.json#/facts/67; endpoint types: /individuals/58/type; /individuals/7/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	causally_upstream_of	C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9822 RO:0002411 gomodel:RXN3O-9822. Source location `./YeastPathways_PWY-6074-1.json#/facts/68; endpoint types: /individuals/58/type; /individuals/58/type`
ERG25 Scer `SGD:S000003292`	enables	C-4 methylsterol oxidase activity `gomodel:RXN3O-9825`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9825 RO:0002333 gomodel:YGR060W-MONOMER_RXN3O-9825_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/69; endpoint types: /individuals/61/type; /individuals/74/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9821`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9821 RO:0002233 gomodel:CHEBI_15379_RXN3O-9821. Source location `./YeastPathways_PWY-6074-1.json#/facts/70; endpoint types: /individuals/57/type; /individuals/30/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9824`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9824 RO:0002234 gomodel:CHEBI_15377_RXN3O-9824. Source location `./YeastPathways_PWY-6074-1.json#/facts/71; endpoint types: /individuals/60/type; /individuals/22/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-313`	has_input	4beta-methylzymosterol-4alpha-carboxylate `CHEBI:64925`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-313 RO:0002233 gomodel:CHEBI_64925_RXN3O-9823. Source location `./YeastPathways_PWY-6074-1.json#/facts/72; endpoint types: /individuals/64/type; /individuals/53/type`
sterol 14-demethylase activity `gomodel:RXN3O-9820`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 RO:0002234 gomodel:CHEBI_15377_RXN3O-9820. Source location `./YeastPathways_PWY-6074-1.json#/facts/73; endpoint types: /individuals/56/type; /individuals/18/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-318`	has_output	zymosterone `CHEBI:52386`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-318 RO:0002234 gomodel:CHEBI_52386_RXN66-318. Source location `./YeastPathways_PWY-6074-1.json#/facts/74; endpoint types: /individuals/66/type; /individuals/44/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9823`	has_output	4beta-methylzymosterol-4alpha-carboxylate `CHEBI:64925`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9823 RO:0002234 gomodel:CHEBI_64925_RXN3O-9823. Source location `./YeastPathways_PWY-6074-1.json#/facts/75; endpoint types: /individuals/59/type; /individuals/53/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9823`	has_input	4beta-methylzymosterol-4-carbaldehyde `CHEBI:87287`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9823 RO:0002233 gomodel:CHEBI_87287_RXN3O-9822. Source location `./YeastPathways_PWY-6074-1.json#/facts/76; endpoint types: /individuals/59/type; /individuals/54/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-314`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-314 RO:0002233 gomodel:CHEBI_15378_RXN66-314. Source location `./YeastPathways_PWY-6074-1.json#/facts/77; endpoint types: /individuals/65/type; /individuals/27/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-314`	has_output	4alpha-methylzymosterol `CHEBI:1949`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-314 RO:0002234 gomodel:CHEBI_1949_RXN66-314. Source location `./YeastPathways_PWY-6074-1.json#/facts/78; endpoint types: /individuals/65/type; /individuals/43/type`
sterol 14-demethylase activity `gomodel:RXN3O-9820`	has_input	lanosterol `CHEBI:16521`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 RO:0002233 gomodel:CHEBI_16521_RXN3O-9820. Source location `./YeastPathways_PWY-6074-1.json#/facts/79; endpoint types: /individuals/56/type; /individuals/37/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9825`	has_output	NAD(P)(+) `CHEBI:13390`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9825 RO:0002234 gomodel:CHEBI_13390_RXN3O-9825. Source location `./YeastPathways_PWY-6074-1.json#/facts/80; endpoint types: /individuals/61/type; /individuals/4/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-318`	has_input	4alpha-carboxyzymosterol(1-) `CHEBI:143575`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-318 RO:0002233 gomodel:CHEBI_143575_RXN3O-9826. Source location `./YeastPathways_PWY-6074-1.json#/facts/81; endpoint types: /individuals/66/type; /individuals/15/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9821`	has_input	NAD(P)H `CHEBI:13392`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9821 RO:0002233 gomodel:CHEBI_13392_RXN3O-9821. Source location `./YeastPathways_PWY-6074-1.json#/facts/82; endpoint types: /individuals/57/type; /individuals/6/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9826`	has_input	4alpha-formylzymosterol `CHEBI:146131`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9826 RO:0002233 gomodel:CHEBI_146131_RXN3O-9825. Source location `./YeastPathways_PWY-6074-1.json#/facts/83; endpoint types: /individuals/62/type; /individuals/17/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9826`	has_input	NAD(P)H `CHEBI:13392`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9826 RO:0002233 gomodel:CHEBI_13392_RXN3O-9826. Source location `./YeastPathways_PWY-6074-1.json#/facts/84; endpoint types: /individuals/62/type; /individuals/11/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-314`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-314 RO:0002234 gomodel:CHEBI_58349_RXN66-314. Source location `./YeastPathways_PWY-6074-1.json#/facts/85; endpoint types: /individuals/65/type; /individuals/51/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-319`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-319 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/87; endpoint types: /individuals/67/type; /individuals/186/type`
Delta14-sterol reductase activity `gomodel:RXN66-306`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-306 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/88; endpoint types: /individuals/63/type; /individuals/186/type`
ERG25 Scer `SGD:S000003292`	enables	C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9822 RO:0002333 gomodel:YGR060W-MONOMER_RXN3O-9822_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/89; endpoint types: /individuals/58/type; /individuals/71/type`
Delta14-sterol reductase activity `gomodel:RXN66-306`	has_output	14-demethyllanosterol `CHEBI:18364`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-306 RO:0002234 gomodel:CHEBI_18364_RXN66-306. Source location `./YeastPathways_PWY-6074-1.json#/facts/90; endpoint types: /individuals/63/type; /individuals/42/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-318`	has_input	NAD(P)(+) `CHEBI:13390`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-318 RO:0002233 gomodel:CHEBI_13390_RXN3O-9826. Source location `./YeastPathways_PWY-6074-1.json#/facts/91; endpoint types: /individuals/66/type; /individuals/5/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-313`	has_input	NAD(P)(+) `CHEBI:13390`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-313 RO:0002233 gomodel:CHEBI_13390_RXN3O-9823. Source location `./YeastPathways_PWY-6074-1.json#/facts/92; endpoint types: /individuals/64/type; /individuals/2/type`
ERG26 Scer `SGD:S000002969`	enables	3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-318`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-318 RO:0002333 gomodel:YGL001C-MONOMER_RXN66-318_controller. Source location `./YeastPathways_PWY-6074-1.json#/facts/93; endpoint types: /individuals/66/type; /individuals/69/type`
sterol 14-demethylase activity `gomodel:RXN3O-9820`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9820 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/94; endpoint types: /individuals/56/type; /individuals/186/type`
Delta14-sterol reductase activity `gomodel:RXN66-306`	has_input	4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol `CHEBI:17813`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-306 RO:0002233 gomodel:CHEBI_17813_RXN3O-9820. Source location `./YeastPathways_PWY-6074-1.json#/facts/95; endpoint types: /individuals/63/type; /individuals/40/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9823`	has_output	NAD(P)(+) `CHEBI:13390`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9823 RO:0002234 gomodel:CHEBI_13390_RXN3O-9823. Source location `./YeastPathways_PWY-6074-1.json#/facts/96; endpoint types: /individuals/59/type; /individuals/2/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9824`	has_output	4alpha-hydroxymethylzymosterol `CHEBI:146130`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9824 RO:0002234 gomodel:CHEBI_146130_RXN3O-9824. Source location `./YeastPathways_PWY-6074-1.json#/facts/97; endpoint types: /individuals/60/type; /individuals/16/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9825`	part_of	zymosterol biosynthetic process `GO:0036197`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9825 BFO:0000050 gomodel:YeastPathways_PWY-6074-1/YeastPathways_PWY-6074-1. Source location `./YeastPathways_PWY-6074-1.json#/facts/98; endpoint types: /individuals/61/type; /individuals/186/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-313`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-313 RO:0002234 gomodel:CHEBI_16526_RXN66-313. Source location `./YeastPathways_PWY-6074-1.json#/facts/99; endpoint types: /individuals/64/type; /individuals/38/type`
3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity `gomodel:RXN66-313`	has_output	3-dehydro-4alpha-methylzymosterol `CHEBI:136486`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-313 RO:0002234 gomodel:CHEBI_136486_RXN66-313. Source location `./YeastPathways_PWY-6074-1.json#/facts/100; endpoint types: /individuals/64/type; /individuals/14/type`
Delta14-sterol reductase activity `gomodel:RXN66-306`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-306 RO:0002233 gomodel:CHEBI_15378_RXN66-306. Source location `./YeastPathways_PWY-6074-1.json#/facts/101; endpoint types: /individuals/63/type; /individuals/26/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9822`	has_output	4beta-methylzymosterol-4-carbaldehyde `CHEBI:87287`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9822 RO:0002234 gomodel:CHEBI_87287_RXN3O-9822. Source location `./YeastPathways_PWY-6074-1.json#/facts/102; endpoint types: /individuals/58/type; /individuals/54/type`
C-4 methylsterol oxidase activity `gomodel:RXN3O-9824`	has_input	NAD(P)H `CHEBI:13392`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN3O-9824 RO:0002233 gomodel:CHEBI_13392_RXN3O-9824. Source location `./YeastPathways_PWY-6074-1.json#/facts/103; endpoint types: /individuals/60/type; /individuals/9/type`
3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity `gomodel:RXN66-319`	has_output	zymosterol `CHEBI:18252`	gomodel:YeastPathways_PWY-6074-1 Source assertion: The model asserts gomodel:RXN66-319 RO:0002234 gomodel:CHEBI_18252_RXN66-319. Source location `./YeastPathways_PWY-6074-1.json#/facts/104; endpoint types: /individuals/67/type; /individuals/41/type`
ERG25 Scer `SGD:S000003292`	located_in Physical protein location from reviewed UniProt. ECO:0000305 (PubMed:12119386); ECO:0000305 (PubMed:15716577); ECO:0000305 (PubMed:8663358) Topology: Single-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P53045 Source assertion: UniProtKB:P53045 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2096/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P53045 Source assertion: UniProtKB:P53045 has the exact SGD cross-reference SGD:S000003292. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2096/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG11 Scer `SGD:S000001049`	located_in Physical protein location from reviewed UniProt. ECO:0000305 Topology: Single-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P10614 Source assertion: UniProtKB:P10614 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/308/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P10614 Source assertion: UniProtKB:P10614 has the exact SGD cross-reference SGD:S000001049. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/308/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG27 Scer `SGD:S000004090`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:12119386); ECO:0000269 (PubMed:12842197); ECO:0000269 (PubMed:15716577) Topology: Peripheral membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:Q12452 Source assertion: UniProtKB:Q12452 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3106/comments/2/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q12452 Source assertion: UniProtKB:Q12452 has the exact SGD cross-reference SGD:S000004090. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3106/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG27 Scer `SGD:S000004090`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:12119386); ECO:0000269 (PubMed:12842197); ECO:0000269 (PubMed:15716577)	lipid droplet `GO:0005811`	UniProtKB:Q12452 Source assertion: UniProtKB:Q12452 annotates location Lipid droplet (SL-0154). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3106/comments/2/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q12452 Source assertion: UniProtKB:Q12452 has the exact SGD cross-reference SGD:S000004090. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3106/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG24 Scer `SGD:S000005224`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:16847258) Topology: Multi-pass membrane protein.	membrane `GO:0016020`	UniProtKB:P32462 Source assertion: UniProtKB:P32462 annotates location Membrane (SL-0162). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/909/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32462 Source assertion: UniProtKB:P32462 has the exact SGD cross-reference SGD:S000005224. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/909/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG26 Scer `SGD:S000002969`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:11279045); ECO:0000269 (PubMed:12119386); ECO:0000269 (PubMed:15716577) Topology: Peripheral membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P53199 Source assertion: UniProtKB:P53199 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2156/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P53199 Source assertion: UniProtKB:P53199 has the exact SGD cross-reference SGD:S000002969. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2156/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG25 Scer `SGD:S000003292`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:8663358)	iron cation `CHEBI:24875`	UniProtKB:P53045 Source assertion: UniProtKB:P53045 annotates CHEBI:24875 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2096/comments/0/cofactors/0` UniProtKB:P53045 Source assertion: UniProtKB:P53045 has the exact SGD cross-reference SGD:S000003292. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2096/uniProtKBCrossReferences/0`
ERG11 Scer `SGD:S000001049`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:24613931)	heme `CHEBI:30413`	UniProtKB:P10614 Source assertion: UniProtKB:P10614 annotates CHEBI:30413 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/308/comments/0/cofactors/0` UniProtKB:P10614 Source assertion: UniProtKB:P10614 has the exact SGD cross-reference SGD:S000001049. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/308/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWY-6074-1 GO-CAM zymosterol biosynthesis model YeastPathways_PWY-6074-1 (source version: Pinned Noctua JSON archive; model date 2024-09-12; member ./YeastPathways_PWY-6074-1.json)
UniProtKB:P53045 Reviewed UniProtKB P53045 location annotations (source version: UniProt release 2026_03)
UniProtKB:P10614 Reviewed UniProtKB P10614 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q12452 Reviewed UniProtKB Q12452 location annotations (source version: UniProt release 2026_03)
UniProtKB:P32462 Reviewed UniProtKB P32462 location annotations (source version: UniProt release 2026_03)
UniProtKB:P53199 Reviewed UniProtKB P53199 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 12 activity location assignments and 5 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 6 independently supported location edges and quarantined 12 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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