ergosterol biosynthesis I

gomodel:YeastPathways_PWY-6075-1

Ergosterol biosynthesis I in Saccharomyces cerevisiae S288C converts zymosterol to ergosterol through ERG6, ERG2, ERG3, ERG5, and ERG4 sterol methyltransferase, isomerase, desaturase, and reductase activities.

Pathway type

lipid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

29 nodes · 47 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
ergosta-5,7,22,24(28)-tetraen-3beta-ol `CHEBI:18249`	lipid
ergosterol `CHEBI:16933`	lipid
ergosterol biosynthetic process `GO:0006696`	biological process
9xi-episterol `CHEBI:50586`	lipid
ERG3 Scer `SGD:S000004046`	protein
NADP(3-) `CHEBI:58349`	small molecule
cytosol `GO:0005829`	cellular component
ERG4 Scer `SGD:S000002980`	protein
water `CHEBI:15377`	small molecule
ERG2 Scer `SGD:S000004815`	protein
dioxygen `CHEBI:15379`	small molecule
NADPH(4-) `CHEBI:57783`	small molecule
zymosterol `CHEBI:18252`	lipid
hydron `CHEBI:15378`	small molecule
fecosterol `CHEBI:17038`	lipid
ergosta-5,7,24(28)-trien-3beta-ol `CHEBI:52972`	lipid
ERG6 Scer `SGD:S000004467`	protein
S-adenosyl-L-methionine zwitterion `CHEBI:59789`	small molecule
ERG5 Scer `SGD:S000004617`	protein
S-adenosyl-L-homocysteine zwitterion `CHEBI:57856`	small molecule
endoplasmic reticulum membrane `GO:0005789`	cellular component
mitochondrion `GO:0005739`	cellular component
iron cation `CHEBI:24875`	cofactor
heme `CHEBI:30413`	cofactor
Delta24(24-1) sterol reductase activity `gomodel:1.3.1.71-RXN`	molecular activity
sterol 24-C-methyltransferase activity `gomodel:RXN3O-178`	molecular activity
C-8 sterol isomerase activity `gomodel:RXN3O-203`	molecular activity
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	molecular activity
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
sterol 24-C-methyltransferase activity `gomodel:RXN3O-178`	provides_input_for	C-8 sterol isomerase activity `gomodel:RXN3O-203`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-178 RO:0002413 gomodel:RXN3O-203. Source location `./YeastPathways_PWY-6075-1.json#/facts/0; endpoint types: /individuals/24/type; /individuals/25/type`
Delta24(24-1) sterol reductase activity `gomodel:1.3.1.71-RXN`	has_input	ergosta-5,7,22,24(28)-tetraen-3beta-ol `CHEBI:18249`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:1.3.1.71-RXN RO:0002233 gomodel:CHEBI_18249_RXN3O-9828. Source location `./YeastPathways_PWY-6075-1.json#/facts/1; endpoint types: /individuals/72/type; /individuals/10/type`
Delta24(24-1) sterol reductase activity `gomodel:1.3.1.71-RXN`	has_output	ergosterol `CHEBI:16933`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:1.3.1.71-RXN RO:0002234 gomodel:CHEBI_16933_1.3.1.71-RXN. Source location `./YeastPathways_PWY-6075-1.json#/facts/2; endpoint types: /individuals/72/type; /individuals/8/type`
Delta24(24-1) sterol reductase activity `gomodel:1.3.1.71-RXN`	part_of	ergosterol biosynthetic process `GO:0006696`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:1.3.1.71-RXN BFO:0000050 gomodel:YeastPathways_PWY-6075-1/YeastPathways_PWY-6075-1. Source location `./YeastPathways_PWY-6075-1.json#/facts/3; endpoint types: /individuals/72/type; /individuals/73/type`
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	has_input	9xi-episterol `CHEBI:50586`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002233 gomodel:CHEBI_50586_RXN3O-203. Source location `./YeastPathways_PWY-6075-1.json#/facts/4; endpoint types: /individuals/26/type; /individuals/12/type`
ERG3 Scer `SGD:S000004046`	enables	delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002333 gomodel:YLR056W-MONOMER_RXN3O-9827_controller. Source location `./YeastPathways_PWY-6075-1.json#/facts/5; endpoint types: /individuals/26/type; /individuals/29/type`
Delta24(24-1) sterol reductase activity `gomodel:1.3.1.71-RXN`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:1.3.1.71-RXN RO:0002234 gomodel:CHEBI_58349_1.3.1.71-RXN. Source location `./YeastPathways_PWY-6075-1.json#/facts/6; endpoint types: /individuals/72/type; /individuals/18/type`
ERG4 Scer `SGD:S000002980`	enables	Delta24(24-1) sterol reductase activity `gomodel:1.3.1.71-RXN`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:1.3.1.71-RXN RO:0002333 gomodel:YGL012W-MONOMER_1.3.1.71-RXN_controller. Source location `./YeastPathways_PWY-6075-1.json#/facts/8; endpoint types: /individuals/72/type; /individuals/28/type`
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002234 gomodel:CHEBI_58349_RXN3O-9828. Source location `./YeastPathways_PWY-6075-1.json#/facts/9; endpoint types: /individuals/27/type; /individuals/20/type`
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	part_of	ergosterol biosynthetic process `GO:0006696`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 BFO:0000050 gomodel:YeastPathways_PWY-6075-1/YeastPathways_PWY-6075-1. Source location `./YeastPathways_PWY-6075-1.json#/facts/10; endpoint types: /individuals/27/type; /individuals/73/type`
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002234 gomodel:CHEBI_15377_RXN3O-9827. Source location `./YeastPathways_PWY-6075-1.json#/facts/11; endpoint types: /individuals/26/type; /individuals/0/type` gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002234 gomodel:YeastPathways_PWY-6075-1/654d809000002548. Source location `./YeastPathways_PWY-6075-1.json#/facts/21; endpoint types: /individuals/26/type; /individuals/78/type`
ERG2 Scer `SGD:S000004815`	enables	C-8 sterol isomerase activity `gomodel:RXN3O-203`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-203 RO:0002333 gomodel:YMR202W-MONOMER_RXN3O-203_controller. Source location `./YeastPathways_PWY-6075-1.json#/facts/12; endpoint types: /individuals/25/type; /individuals/30/type`
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002233 gomodel:CHEBI_15379_RXN3O-9827. Source location `./YeastPathways_PWY-6075-1.json#/facts/13; endpoint types: /individuals/26/type; /individuals/6/type`
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002233 gomodel:CHEBI_57783_RXN3O-9827. Source location `./YeastPathways_PWY-6075-1.json#/facts/14; endpoint types: /individuals/26/type; /individuals/15/type`
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002233 gomodel:CHEBI_57783_RXN3O-9828. Source location `./YeastPathways_PWY-6075-1.json#/facts/15; endpoint types: /individuals/27/type; /individuals/16/type`
sterol 24-C-methyltransferase activity `gomodel:RXN3O-178`	has_input	zymosterol `CHEBI:18252`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-178 RO:0002233 gomodel:CHEBI_18252_RXN3O-178. Source location `./YeastPathways_PWY-6075-1.json#/facts/16; endpoint types: /individuals/24/type; /individuals/11/type`
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	has_output	ergosta-5,7,22,24(28)-tetraen-3beta-ol `CHEBI:18249`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002234 gomodel:CHEBI_18249_RXN3O-9828. Source location `./YeastPathways_PWY-6075-1.json#/facts/17; endpoint types: /individuals/27/type; /individuals/10/type`
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002234 gomodel:YeastPathways_PWY-6075-1/654d809000002553. Source location `./YeastPathways_PWY-6075-1.json#/facts/18; endpoint types: /individuals/27/type; /individuals/80/type` gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002234 gomodel:CHEBI_15377_RXN3O-9828. Source location `./YeastPathways_PWY-6075-1.json#/facts/34; endpoint types: /individuals/27/type; /individuals/1/type`
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002233 gomodel:CHEBI_15378_RXN3O-9828. Source location `./YeastPathways_PWY-6075-1.json#/facts/19; endpoint types: /individuals/27/type; /individuals/5/type`
Delta24(24-1) sterol reductase activity `gomodel:1.3.1.71-RXN`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:1.3.1.71-RXN RO:0002233 gomodel:CHEBI_57783_1.3.1.71-RXN. Source location `./YeastPathways_PWY-6075-1.json#/facts/20; endpoint types: /individuals/72/type; /individuals/14/type`
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	provides_input_for	Delta24(24-1) sterol reductase activity `gomodel:1.3.1.71-RXN`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002413 gomodel:1.3.1.71-RXN. Source location `./YeastPathways_PWY-6075-1.json#/facts/22; endpoint types: /individuals/27/type; /individuals/72/type`
C-8 sterol isomerase activity `gomodel:RXN3O-203`	part_of	ergosterol biosynthetic process `GO:0006696`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-203 BFO:0000050 gomodel:YeastPathways_PWY-6075-1/YeastPathways_PWY-6075-1. Source location `./YeastPathways_PWY-6075-1.json#/facts/24; endpoint types: /individuals/25/type; /individuals/73/type`
sterol 24-C-methyltransferase activity `gomodel:RXN3O-178`	has_output	fecosterol `CHEBI:17038`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-178 RO:0002234 gomodel:CHEBI_17038_RXN3O-178. Source location `./YeastPathways_PWY-6075-1.json#/facts/26; endpoint types: /individuals/24/type; /individuals/9/type`
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002233 gomodel:CHEBI_15378_RXN3O-9827. Source location `./YeastPathways_PWY-6075-1.json#/facts/27; endpoint types: /individuals/26/type; /individuals/4/type`
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002234 gomodel:CHEBI_58349_RXN3O-9827. Source location `./YeastPathways_PWY-6075-1.json#/facts/28; endpoint types: /individuals/26/type; /individuals/19/type`
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	part_of	ergosterol biosynthetic process `GO:0006696`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 BFO:0000050 gomodel:YeastPathways_PWY-6075-1/YeastPathways_PWY-6075-1. Source location `./YeastPathways_PWY-6075-1.json#/facts/29; endpoint types: /individuals/26/type; /individuals/73/type`
Delta24(24-1) sterol reductase activity `gomodel:1.3.1.71-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:1.3.1.71-RXN RO:0002233 gomodel:CHEBI_15378_1.3.1.71-RXN. Source location `./YeastPathways_PWY-6075-1.json#/facts/30; endpoint types: /individuals/72/type; /individuals/2/type`
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	has_input	ergosta-5,7,24(28)-trien-3beta-ol `CHEBI:52972`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002233 gomodel:CHEBI_52972_RXN3O-9827. Source location `./YeastPathways_PWY-6075-1.json#/facts/32; endpoint types: /individuals/27/type; /individuals/13/type`
ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002233 gomodel:CHEBI_15379_RXN3O-9828. Source location `./YeastPathways_PWY-6075-1.json#/facts/33; endpoint types: /individuals/27/type; /individuals/7/type`
C-8 sterol isomerase activity `gomodel:RXN3O-203`	has_output	9xi-episterol `CHEBI:50586`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-203 RO:0002234 gomodel:CHEBI_50586_RXN3O-203. Source location `./YeastPathways_PWY-6075-1.json#/facts/35; endpoint types: /individuals/25/type; /individuals/12/type`
sterol 24-C-methyltransferase activity `gomodel:RXN3O-178`	part_of	ergosterol biosynthetic process `GO:0006696`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-178 BFO:0000050 gomodel:YeastPathways_PWY-6075-1/YeastPathways_PWY-6075-1. Source location `./YeastPathways_PWY-6075-1.json#/facts/36; endpoint types: /individuals/24/type; /individuals/73/type`
ERG6 Scer `SGD:S000004467`	enables	sterol 24-C-methyltransferase activity `gomodel:RXN3O-178`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-178 RO:0002333 gomodel:MONOMER3O-188_RXN3O-178_controller. Source location `./YeastPathways_PWY-6075-1.json#/facts/37; endpoint types: /individuals/24/type; /individuals/22/type`
C-8 sterol isomerase activity `gomodel:RXN3O-203`	has_input	fecosterol `CHEBI:17038`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-203 RO:0002233 gomodel:CHEBI_17038_RXN3O-178. Source location `./YeastPathways_PWY-6075-1.json#/facts/38; endpoint types: /individuals/25/type; /individuals/9/type`
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	provides_input_for	ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002413 gomodel:RXN3O-9828. Source location `./YeastPathways_PWY-6075-1.json#/facts/40; endpoint types: /individuals/26/type; /individuals/27/type`
C-8 sterol isomerase activity `gomodel:RXN3O-203`	provides_input_for	delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-203 RO:0002413 gomodel:RXN3O-9827. Source location `./YeastPathways_PWY-6075-1.json#/facts/41; endpoint types: /individuals/25/type; /individuals/26/type`
delta7-sterol 5(6)-desaturase activity `gomodel:RXN3O-9827`	has_output	ergosta-5,7,24(28)-trien-3beta-ol `CHEBI:52972`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9827 RO:0002234 gomodel:CHEBI_52972_RXN3O-9827. Source location `./YeastPathways_PWY-6075-1.json#/facts/42; endpoint types: /individuals/26/type; /individuals/13/type`
sterol 24-C-methyltransferase activity `gomodel:RXN3O-178`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-178 RO:0002234 gomodel:CHEBI_15378_RXN3O-178. Source location `./YeastPathways_PWY-6075-1.json#/facts/43; endpoint types: /individuals/24/type; /individuals/3/type`
sterol 24-C-methyltransferase activity `gomodel:RXN3O-178`	has_input	S-adenosyl-L-methionine zwitterion `CHEBI:59789`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-178 RO:0002233 gomodel:CHEBI_59789_RXN3O-178. Source location `./YeastPathways_PWY-6075-1.json#/facts/44; endpoint types: /individuals/24/type; /individuals/21/type`
ERG5 Scer `SGD:S000004617`	enables	ergosta-5,7,24(28)-trien-3beta-ol desaturation `gomodel:RXN3O-9828`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-9828 RO:0002333 gomodel:MONOMER3O-232_RXN3O-9828_controller. Source location `./YeastPathways_PWY-6075-1.json#/facts/45; endpoint types: /individuals/27/type; /individuals/23/type`
sterol 24-C-methyltransferase activity `gomodel:RXN3O-178`	has_output	S-adenosyl-L-homocysteine zwitterion `CHEBI:57856`	gomodel:YeastPathways_PWY-6075-1 Source assertion: The model asserts gomodel:RXN3O-178 RO:0002234 gomodel:CHEBI_57856_RXN3O-178. Source location `./YeastPathways_PWY-6075-1.json#/facts/46; endpoint types: /individuals/24/type; /individuals/17/type`
ERG3 Scer `SGD:S000004046`	located_in Physical protein location from reviewed UniProt. ECO:0000305 Topology: Multi-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P32353 Source assertion: UniProtKB:P32353 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/885/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32353 Source assertion: UniProtKB:P32353 has the exact SGD cross-reference SGD:S000004046. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/885/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG4 Scer `SGD:S000002980`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:10722850) Topology: Multi-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P25340 Source assertion: UniProtKB:P25340 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/671/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P25340 Source assertion: UniProtKB:P25340 has the exact SGD cross-reference SGD:S000002980. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/671/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG2 Scer `SGD:S000004815`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:15716577) Topology: Single-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P32352 Source assertion: UniProtKB:P32352 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/884/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32352 Source assertion: UniProtKB:P32352 has the exact SGD cross-reference SGD:S000004815. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/884/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG6 Scer `SGD:S000004467`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:6363386)	mitochondrion `GO:0005739`	UniProtKB:P25087 Source assertion: UniProtKB:P25087 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/658/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P25087 Source assertion: UniProtKB:P25087 has the exact SGD cross-reference SGD:S000004467. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/658/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG5 Scer `SGD:S000004617`	located_in Physical protein location from reviewed UniProt. ECO:0000305 Topology: Single-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P54781 Source assertion: UniProtKB:P54781 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2328/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P54781 Source assertion: UniProtKB:P54781 has the exact SGD cross-reference SGD:S000004617. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2328/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ERG3 Scer `SGD:S000004046`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P53045)	iron cation `CHEBI:24875`	UniProtKB:P32353 Source assertion: UniProtKB:P32353 annotates CHEBI:24875 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/885/comments/0/cofactors/0` UniProtKB:P32353 Source assertion: UniProtKB:P32353 has the exact SGD cross-reference SGD:S000004046. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/885/uniProtKBCrossReferences/0`
ERG5 Scer `SGD:S000004617`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P04798)	heme `CHEBI:30413`	UniProtKB:P54781 Source assertion: UniProtKB:P54781 annotates CHEBI:30413 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2328/comments/0/cofactors/0` UniProtKB:P54781 Source assertion: UniProtKB:P54781 has the exact SGD cross-reference SGD:S000004617. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2328/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWY-6075-1 GO-CAM ergosterol biosynthesis I model YeastPathways_PWY-6075-1 (source version: Pinned Noctua JSON archive; model date 2026-08-03; member ./YeastPathways_PWY-6075-1.json)
UniProtKB:P32353 Reviewed UniProtKB P32353 location annotations (source version: UniProt release 2026_03)
UniProtKB:P25340 Reviewed UniProtKB P25340 location annotations (source version: UniProt release 2026_03)
UniProtKB:P32352 Reviewed UniProtKB P32352 location annotations (source version: UniProt release 2026_03)
UniProtKB:P25087 Reviewed UniProtKB P25087 location annotations (source version: UniProt release 2026_03)
UniProtKB:P54781 Reviewed UniProtKB P54781 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 5 activity location assignments and 5 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 5 independently supported location edges and quarantined 5 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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