thiamine biosynthesis

gomodel:YeastPathways_PWY3O-17

Thiamine biosynthesis in Saccharomyces cerevisiae S288C builds the pyrimidine and thiazole branches, condenses 4-amino-2-methyl-5-diphosphonatooxymethylpyrimidine and 4-methyl-5-(2-phosphonatooxyethyl)thiazole to thiamine monophosphate, and dephosphorylates and diphosphorylates it to thiamine diphosphate.

Pathway type
cofactor biosynthesis
Organism scope

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Pathway network

46 nodes · 95 asserted directed edges

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Pathway network: thiamine biosynthesis 46 nodes · 95 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. gomodel:RXNQT-4301 — has_output → CHEBI:456215; Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXNQT-4301 — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of gomodel:PYRIMSYN3-RXN — has_output → CHEBI:456216; Evidence: gomodel:YeastPathways_PWY3O-17has output SGD:S000006179 — enables → gomodel:YeastPathways_PWY3O-17/6a4c244800007660; Evidence: gomodel:YeastPathways_PWY3O-17enables gomodel:RXNQT-4301 — has_output → CHEBI:58296; Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXNQT-4191 — has_input → CHEBI:37575; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:YeastPathways_PWY3O-17/6a4c244800007677 — has_output → CHEBI:456216; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXNQT-4301 — has_input → CHEBI:139151; Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.; Evidence: gomodel:YeastPathways_PWY3O-17has input SGD:S000005416 — enables → gomodel:PYRIMSYN3-RXN; Evidence: gomodel:YeastPathways_PWY3O-17enables gomodel:YeastPathways_PWY3O-17/6a4c244800007660 — has_input → CHEBI:16892; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:RXNQT-4191 — has_output → CHEBI:43474; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXN3O-401 — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of gomodel:THI-P-SYN-RXN — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of gomodel:RXNQT-4191 — has_input → CHEBI:15377; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of gomodel:YeastPathways_PWY3O-17/6a4c244800007660 — has_input → CHEBI:30616; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:RXNQT-4191 — has_output → CHEBI:18385; Evidence: gomodel:YeastPathways_PWY3O-17has output SGD:S000000296 — enables → gomodel:RXNQT-4191; Evidence: gomodel:YeastPathways_PWY3O-17enables gomodel:RXNQT-4301 — has_output → CHEBI:16526; Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXN3O-9804 — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:OHMETPYRKIN-RXN — provides_input_for → gomodel:YeastPathways_PWY3O-17/6a4c244800007677; Evidence: gomodel:YeastPathways_PWY3O-17provides input for gomodel:PYRIMSYN3-RXN — has_input → CHEBI:30616; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:THI-P-SYN-RXN — has_output → CHEBI:29888; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:OHMETPYRKIN-RXN — has_output → CHEBI:456216; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:THI-P-SYN-RXN — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN — has_output → CHEBI:58937; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:THI-P-SYN-RXN — has_input → CHEBI:15378; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:YeastPathways_PWY3O-17/6a4c244800007660 — has_output → CHEBI:456216; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:PYRIMSYN3-RXN — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN — has_input → CHEBI:18385; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:YeastPathways_PWY3O-17/6a4c244800007660 — has_output → CHEBI:15378; Evidence: gomodel:YeastPathways_PWY3O-17has output SGD:S000006179 — enables → gomodel:YeastPathways_PWY3O-17/6a4c244800007677; Evidence: gomodel:YeastPathways_PWY3O-17enables gomodel:THI-P-SYN-RXN — has_input → CHEBI:58296; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:OHMETPYRKIN-RXN — has_input → CHEBI:30616; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:YeastPathways_PWY3O-17/6a4c244800007677 — has_output → CHEBI:57841; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:YeastPathways_PWY3O-17/6a4c244800007677 — has_input → CHEBI:30616; Evidence: gomodel:YeastPathways_PWY3O-17has input SGD:S000005669 — enables → gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN; Evidence: gomodel:YeastPathways_PWY3O-17enables gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN — has_output → CHEBI:456215; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXN3O-9804 — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of gomodel:RXN3O-401 — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:RXN3O-401 — provides_input_for → gomodel:RXNQT-4301; Evidence: gomodel:YeastPathways_PWY3O-17provides input for gomodel:OHMETPYRKIN-RXN — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:RXNQT-4191 — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of gomodel:YeastPathways_PWY3O-17/6a4c244800007677 — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:PYRIMSYN3-RXN — has_input → CHEBI:58354; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:RXNQT-4301 — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:YeastPathways_PWY3O-17/6a4c244800007660 — has_output → CHEBI:58354; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXNQT-4301 — has_output → CHEBI:15378; Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:OHMETPYRKIN-RXN — has_output → CHEBI:15378; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXNQT-4301 — provides_input_for → gomodel:THI-P-SYN-RXN; Evidence: gomodel:YeastPathways_PWY3O-17provides input for gomodel:YeastPathways_PWY3O-17/6a4c244800007677 — provides_input_for → gomodel:THI-P-SYN-RXN; Evidence: gomodel:YeastPathways_PWY3O-17provides input for gomodel:YeastPathways_PWY3O-17/6a4c244800007660 — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN — has_input → CHEBI:30616; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN — has_output → CHEBI:15378; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXNQT-4191 — provides_input_for → gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN; Evidence: gomodel:YeastPathways_PWY3O-17provides input for gomodel:YeastPathways_PWY3O-17/6a4c244800007660 — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of gomodel:OHMETPYRKIN-RXN — has_output → CHEBI:58354; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:PYRIMSYN3-RXN — provides_input_for → gomodel:THI-P-SYN-RXN; Evidence: gomodel:YeastPathways_PWY3O-17provides input for gomodel:OHMETPYRKIN-RXN — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of SGD:S000006135 — enables → gomodel:THI-P-SYN-RXN; Evidence: gomodel:YeastPathways_PWY3O-17enables gomodel:PYRIMSYN3-RXN — has_output → CHEBI:57841; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:YeastPathways_PWY3O-17/6a4c244800007677 — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of gomodel:THI-P-SYN-RXN — has_output → CHEBI:37575; Evidence: gomodel:YeastPathways_PWY3O-17has output gomodel:RXNQT-4301 — has_input → CHEBI:15377; Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:OHMETPYRKIN-RXN — provides_input_for → gomodel:PYRIMSYN3-RXN; Evidence: gomodel:YeastPathways_PWY3O-17provides input for SGD:S000005416 — enables → gomodel:OHMETPYRKIN-RXN; Evidence: gomodel:YeastPathways_PWY3O-17enables gomodel:YeastPathways_PWY3O-17/6a4c244800007660 — provides_input_for → gomodel:YeastPathways_PWY3O-17/6a4c244800007677; Evidence: gomodel:YeastPathways_PWY3O-17provides input for gomodel:THI-P-SYN-RXN — has_input → CHEBI:57841; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:YeastPathways_PWY3O-17/6a4c244800007677 — has_input → CHEBI:58354; Evidence: gomodel:YeastPathways_PWY3O-17has input gomodel:OHMETPYRKIN-RXN — has_input → CHEBI:16892; Evidence: gomodel:YeastPathways_PWY3O-17has input SGD:S000003376 — enables → gomodel:RXN3O-401; Evidence: gomodel:YeastPathways_PWY3O-17enables gomodel:PYRIMSYN3-RXN — part_of → GO:0009228; Evidence: gomodel:YeastPathways_PWY3O-17part of gomodel:RXNQT-4191 — occurs_in → GO:0005829; Evidence: gomodel:YeastPathways_PWY3O-17occurs in gomodel:YeastPathways_PWY3O-17/6a4c244800007660 — provides_input_for → gomodel:PYRIMSYN3-RXN; Evidence: gomodel:YeastPathways_PWY3O-17provides input for SGD:S000002403 — enables → gomodel:RXN3O-9804; Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.; Evidence: gomodel:YeastPathways_PWY3O-17, UniProtKB:Q07748enables gomodel:THI-P-SYN-RXN — provides_input_for → gomodel:RXNQT-4191; Evidence: gomodel:YeastPathways_PWY3O-17provides input for SGD:S000003376 — located_in → GO:0005737; Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:15544818); ECO:0000269 (PubMed:16850348) Source qualification: Excluded from the vacuole; Evidence: UniProtKB:P32318, UniProtKB:P32318located in SGD:S000003376 — located_in → GO:0005634; Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:16850348) Source qualification: Excluded from the vacuole; Evidence: UniProtKB:P32318, UniProtKB:P32318located in gomodel:RXN3O-9804 — has_input → CHEBI:143915; Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.; Evidence: UniProtKB:Q07748has input gomodel:RXN3O-9804 — has_input → CHEBI:29979; Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.; Evidence: UniProtKB:Q07748has input gomodel:RXN3O-9804 — has_output → CHEBI:58354; Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.; Evidence: UniProtKB:Q07748has output gomodel:RXN3O-9804 — provides_input_for → gomodel:PYRIMSYN3-RXN; Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.; Evidence: UniProtKB:Q07748, gomodel:YeastPathways_PWY3O-17provides input for gomodel:RXN3O-9804 — provides_input_for → gomodel:YeastPathways_PWY3O-17/6a4c244800007677; Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.; Evidence: UniProtKB:Q07748, gomodel:YeastPathways_PWY3O-17provides input for gomodel:RXN3O-401 — has_input → CHEBI:29950; THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.; Evidence: UniProtKB:P32318has input gomodel:RXN3O-401 — has_input → CHEBI:57305; THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.; Evidence: UniProtKB:P32318has input gomodel:RXN3O-401 — has_input → CHEBI:57540; THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.; Evidence: UniProtKB:P32318has input gomodel:RXN3O-401 — has_output → CHEBI:90873; THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.; Evidence: UniProtKB:P32318has output gomodel:RXN3O-401 — has_output → CHEBI:139151; THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.; Evidence: UniProtKB:P32318has output gomodel:RXN3O-401 — has_output → CHEBI:17154; THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.; Evidence: UniProtKB:P32318has output gomodel:RXN3O-401 — has_output → CHEBI:15377; THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.; Evidence: UniProtKB:P32318has output gomodel:RXN3O-401 — has_output → CHEBI:15378; THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.; Evidence: UniProtKB:P32318has output SGD:S000003376 — has_cofactor → CHEBI:24875; UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:22031445) Binds 1 Fe cation per subunit. UniProt note evidence: ECO:0000269 (PubMed:22031445); Evidence: UniProtKB:P32318, UniProtKB:P32318has cofactor SGD:S000006135 — has_cofactor → CHEBI:18420; UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 Mg(2+) ion per subunit. UniProt note evidence: ECO:0000250; Evidence: UniProtKB:P41835, UniProtKB:P41835has cofactor SGD:S000002403 — has_cofactor → CHEBI:24875; UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P43534); Evidence: UniProtKB:Q07748, UniProtKB:Q07748has cofactor adenosine 5'-monophosphate(2-); CHEBI:456215; Declared type: small_molecule small molecule adenosine 5'-monophosphate(2-) thiamine biosynthetic process; GO:0009228; Declared type: biological_process biological process thiamine biosynthetic process ADP(3-); CHEBI:456216; Declared type: small_molecule small molecule ADP(3-) THI21 Scer; SGD:S000006179; Declared type: protein protein THI21 Scer 4-methyl-5-(2-phosphonatooxyethyl)thiazole(2-); CHEBI:58296; Declared type: small_molecule small molecule 4-methyl-5-(2- phosphonatooxyethyl)thiazole (2-) thiamine(1+) monophosphate(2-); CHEBI:37575; Declared type: small_molecule small molecule thiamine(1+) monophosphate(2-) ADP-5-ethyl-4-methylthiazole-2-carboxylate(3-); CHEBI:139151; Declared type: small_molecule small molecule ADP-5-ethyl-4- methylthiazole-2- carboxylate(3-) THI20 Scer; SGD:S000005416; Declared type: protein protein THI20 Scer 4-amino-5-hydroxymethyl-2-methylpyrimidine; CHEBI:16892; Declared type: small_molecule small molecule 4-amino-5-hydroxymethyl-2- methylpyrimidine hydrogenphosphate; CHEBI:43474; Declared type: small_molecule small molecule hydrogenphosphate water; CHEBI:15377; Declared type: small_molecule small molecule water nicotinamide; CHEBI:17154; Declared type: small_molecule small molecule nicotinamide ATP(4-); CHEBI:30616; Declared type: small_molecule small molecule ATP(4-) thiamine(1+); CHEBI:18385; Declared type: small_molecule small molecule thiamine(1+) PHO3 Scer; SGD:S000000296; Declared type: protein protein PHO3 Scer carbon dioxide; CHEBI:16526; Declared type: small_molecule small molecule carbon dioxide cytosol; GO:0005829; Declared type: cellular_component cellular component cytosol diphosphoric acid; CHEBI:29888; Declared type: small_molecule small molecule diphosphoric acid thiamine(1+) diphosphate(3-); CHEBI:58937; Declared type: small_molecule small molecule thiamine(1+) diphosphate(3-) hydron; CHEBI:15378; Declared type: small_molecule small molecule hydron 4-amino-2-methyl-5-diphosphonatooxymethylpyrimidine(3-); CHEBI:57841; Declared type: small_molecule small molecule 4-amino-2-methyl-5- diphosphonatooxymethylpyrimi dine(3-) THI80 Scer; SGD:S000005669; Declared type: protein protein THI80 Scer 4-amino-2-methyl-5-phosphonatooxymethylpyrimidine(2-); CHEBI:58354; Declared type: small_molecule small molecule 4-amino-2-methyl-5- phosphonatooxymethylpyrimidi ne(2-) THI4 Scer; SGD:S000003376; Declared type: protein protein THI4 Scer NAD(1-); CHEBI:57540; Declared type: small_molecule small molecule NAD(1-) THI6 Scer; SGD:S000006135; Declared type: protein protein THI6 Scer glycine zwitterion; CHEBI:57305; Declared type: small_molecule small molecule glycine zwitterion THI13 Scer; SGD:S000002403; Declared type: protein protein THI13 Scer cytoplasm; GO:0005737; Declared type: cellular_component cellular component cytoplasm nucleus; GO:0005634; Declared type: cellular_component cellular component nucleus N(6)-(pyridoxal phosphate)-L-lysine(2-) residue; CHEBI:143915; Declared type: participant (type not recorded) participant · type not recorded N(6)-(pyridoxal phosphate)-L-lysine(2-) residue L-histidine residue; CHEBI:29979; Declared type: participant (type not recorded) participant · type not recorded L-histidine residue L-cysteine residue; CHEBI:29950; Declared type: participant (type not recorded) participant · type not recorded L-cysteine residue dehydroalanine residue; CHEBI:90873; Declared type: participant (type not recorded) participant · type not recorded dehydroalanine residue iron cation; CHEBI:24875; Declared type: cofactor cofactor iron cation magnesium(2+); CHEBI:18420; Declared type: cofactor cofactor magnesium(2+) hydroxymethylpyrimidine kinase activity; gomodel:OHMETPYRKIN-RXN; Declared type: molecular_activity molecular activity hydroxymethylpyrimidine kinase activity phosphomethylpyrimidine kinase activity; gomodel:PYRIMSYN3-RXN; Declared type: molecular_activity molecular activity phosphomethylpyrimidine kinase activity cysteine-dependent adenosine diphosphate thiazole synthase activity; gomodel:RXN3O-401; Declared type: molecular_activity molecular activity cysteine-dependent adenosine diphosphate thiazole synthase activity 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP; gomodel:RXN3O-9804; Declared type: molecular_activity molecular activity 4-amino-5-hydroxymethyl-2- methylpyrimidine phosphate synthase activity from histidine and PLP thiamine phosphate phosphatase activity; gomodel:RXNQT-4191; Declared type: molecular_activity molecular activity thiamine phosphate phosphatase activity ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+; gomodel:RXNQT-4301; Declared type: molecular_activity molecular activity ADP-5-ethyl-4- methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5- (2-phosphooxyethyl)thiazole + CO2 + H+ thiamine-phosphate diphosphorylase activity; gomodel:THI-P-SYN-RXN; Declared type: molecular_activity molecular activity thiamine-phosphate diphosphorylase activity thiamine diphosphokinase activity; gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN; Declared type: molecular_activity molecular activity thiamine diphosphokinase activity hydroxymethylpyrimidine kinase activity; gomodel:YeastPathways_PWY3O-17/6a4c244800007660; Declared type: molecular_activity molecular activity hydroxymethylpyrimidine kinase activity phosphomethylpyrimidine kinase activity; gomodel:YeastPathways_PWY3O-17/6a4c244800007677; Declared type: molecular_activity molecular activity phosphomethylpyrimidine kinase activity

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Saccharomyces cerevisiae S288C
NCBITaxon:559292		taxon
adenosine 5'-monophosphate(2-)
CHEBI:456215		small molecule
thiamine biosynthetic process
GO:0009228		biological process
ADP(3-)
CHEBI:456216		small molecule
THI21 Scer
SGD:S000006179		protein
4-methyl-5-(2-phosphonatooxyethyl)thiazole(2-)
CHEBI:58296		small molecule
thiamine(1+) monophosphate(2-)
CHEBI:37575		small molecule
ADP-5-ethyl-4-methylthiazole-2-carboxylate(3-)
CHEBI:139151		small molecule
THI20 Scer
SGD:S000005416		protein
4-amino-5-hydroxymethyl-2-methylpyrimidine
CHEBI:16892		small molecule
hydrogenphosphate
CHEBI:43474		small molecule
water
CHEBI:15377		small molecule
nicotinamide
CHEBI:17154		small molecule
ATP(4-)
CHEBI:30616		small molecule
thiamine(1+)
CHEBI:18385		small molecule
PHO3 Scer
SGD:S000000296		protein
carbon dioxide
CHEBI:16526		small molecule
cytosol
GO:0005829		cellular component
diphosphoric acid
CHEBI:29888		small molecule
thiamine(1+) diphosphate(3-)
CHEBI:58937		small molecule
hydron
CHEBI:15378		small molecule
4-amino-2-methyl-5-diphosphonatooxymethylpyrimidine(3-)
CHEBI:57841		small molecule
THI80 Scer
SGD:S000005669		protein
4-amino-2-methyl-5-phosphonatooxymethylpyrimidine(2-)
CHEBI:58354		small molecule
THI4 Scer
SGD:S000003376		protein
NAD(1-)
CHEBI:57540		small molecule
THI6 Scer
SGD:S000006135		protein
glycine zwitterion
CHEBI:57305		small molecule
THI13 Scer
SGD:S000002403		protein
cytoplasm
GO:0005737		cellular component
nucleus
GO:0005634		cellular component
N(6)-(pyridoxal phosphate)-L-lysine(2-) residue
CHEBI:143915		participant
L-histidine residue
CHEBI:29979		participant
L-cysteine residue
CHEBI:29950		participant
dehydroalanine residue
CHEBI:90873		participant
iron cation
CHEBI:24875		cofactor
magnesium(2+)
CHEBI:18420		cofactor
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		molecular activity
phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN		molecular activity
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		molecular activity
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
gomodel:RXN3O-9804		molecular activity
thiamine phosphate phosphatase activity
gomodel:RXNQT-4191		molecular activity
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		molecular activity
thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN		molecular activity
thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN		molecular activity
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		molecular activity
phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677		molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		has_output

Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4301 RO:0002234 gomodel:CHEBI_456215_RXNQT-4301.

    Source location./YeastPathways_PWY3O-17.json#/facts/0; endpoint types: /individuals/35/type; /individuals/17/type
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4301 BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/1; endpoint types: /individuals/35/type; /individuals/91/type
phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN		has_output

ADP(3-)
CHEBI:456216
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:PYRIMSYN3-RXN RO:0002234 gomodel:CHEBI_456216_PYRIMSYN3-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/2; endpoint types: /individuals/31/type; /individuals/20/type
THI21 Scer
SGD:S000006179		enables

hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 RO:0002333 gomodel:YeastPathways_PWY3O-17/6a4c244800007665.

    Source location./YeastPathways_PWY3O-17.json#/facts/3; endpoint types: /individuals/103/type; /individuals/107/type
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		has_output

Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.

4-methyl-5-(2-phosphonatooxyethyl)thiazole(2-)
CHEBI:58296
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4301 RO:0002234 gomodel:CHEBI_58296_RXNQT-4301.

    Source location./YeastPathways_PWY3O-17.json#/facts/4; endpoint types: /individuals/35/type; /individuals/25/type
thiamine phosphate phosphatase activity
gomodel:RXNQT-4191		has_input

thiamine(1+) monophosphate(2-)
CHEBI:37575
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4191 RO:0002233 gomodel:CHEBI_37575_THI-P-SYN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/5; endpoint types: /individuals/34/type; /individuals/15/type
phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677		has_output

ADP(3-)
CHEBI:456216
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007677 RO:0002234 gomodel:YeastPathways_PWY3O-17/6a4c244800007679.

    Source location./YeastPathways_PWY3O-17.json#/facts/6; endpoint types: /individuals/119/type; /individuals/121/type
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		has_input

Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.

ADP-5-ethyl-4-methylthiazole-2-carboxylate(3-)
CHEBI:139151
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4301 RO:0002233 gomodel:CHEBI_139151_RXN3O-401.

    Source location./YeastPathways_PWY3O-17.json#/facts/7; endpoint types: /individuals/35/type; /individuals/0/type
THI20 Scer
SGD:S000005416		enables

phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:PYRIMSYN3-RXN RO:0002333 gomodel:YeastPathways_PWY3O-17/6690711d00000649.

    Source location./YeastPathways_PWY3O-17.json#/facts/8; endpoint types: /individuals/31/type; /individuals/136/type
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		has_input

4-amino-5-hydroxymethyl-2-methylpyrimidine
CHEBI:16892
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 RO:0002233 gomodel:CHEBI_16892_RXN3O-9804.

    Source location./YeastPathways_PWY3O-17.json#/facts/9; endpoint types: /individuals/103/type; /individuals/8/type
thiamine phosphate phosphatase activity
gomodel:RXNQT-4191		has_output

hydrogenphosphate
CHEBI:43474
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4191 RO:0002234 gomodel:CHEBI_43474_RXNQT-4191.

    Source location./YeastPathways_PWY3O-17.json#/facts/10; endpoint types: /individuals/34/type; /individuals/16/type
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXN3O-401 BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/11; endpoint types: /individuals/32/type; /individuals/91/type
thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THI-P-SYN-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/12; endpoint types: /individuals/36/type; /individuals/91/type
thiamine phosphate phosphatase activity
gomodel:RXNQT-4191		has_input

water
CHEBI:15377
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4191 RO:0002233 gomodel:CHEBI_15377_RXNQT-4191.

    Source location./YeastPathways_PWY3O-17.json#/facts/13; endpoint types: /individuals/34/type; /individuals/1/type
thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/14; endpoint types: /individuals/37/type; /individuals/91/type
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		has_input

ATP(4-)
CHEBI:30616
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 RO:0002233 gomodel:YeastPathways_PWY3O-17/6a4c244800007661.

    Source location./YeastPathways_PWY3O-17.json#/facts/16; endpoint types: /individuals/103/type; /individuals/104/type
thiamine phosphate phosphatase activity
gomodel:RXNQT-4191		has_output

thiamine(1+)
CHEBI:18385
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4191 RO:0002234 gomodel:CHEBI_18385_RXNQT-4191.

    Source location./YeastPathways_PWY3O-17.json#/facts/17; endpoint types: /individuals/34/type; /individuals/10/type
PHO3 Scer
SGD:S000000296		enables

thiamine phosphate phosphatase activity
gomodel:RXNQT-4191
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4191 RO:0002333 gomodel:YeastPathways_PWY3O-17/6a4c244800007651.

    Source location./YeastPathways_PWY3O-17.json#/facts/18; endpoint types: /individuals/34/type; /individuals/94/type
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		has_output

Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.

carbon dioxide
CHEBI:16526
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4301 RO:0002234 gomodel:CHEBI_16526_RXNQT-4301.

    Source location./YeastPathways_PWY3O-17.json#/facts/19; endpoint types: /individuals/35/type; /individuals/7/type
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
gomodel:RXN3O-9804		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXN3O-9804 BFO:0000066 gomodel:reaction_RXN3O-9804_location_lociGO_0005829.

    Source location./YeastPathways_PWY3O-17.json#/facts/20; endpoint types: /individuals/33/type; /individuals/86/type
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		provides_input_for

phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN RO:0002413 gomodel:YeastPathways_PWY3O-17/6a4c244800007677.

    Source location./YeastPathways_PWY3O-17.json#/facts/21; endpoint types: /individuals/30/type; /individuals/119/type
phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN		has_input

ATP(4-)
CHEBI:30616
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:PYRIMSYN3-RXN RO:0002233 gomodel:CHEBI_30616_PYRIMSYN3-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/22; endpoint types: /individuals/31/type; /individuals/13/type
thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN BFO:0000066 gomodel:reaction_THIAMIN-PYROPHOSPHOKINASE-RXN_location_lociGO_0005829.

    Source location./YeastPathways_PWY3O-17.json#/facts/23; endpoint types: /individuals/37/type; /individuals/90/type
thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN		has_output

diphosphoric acid
CHEBI:29888
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THI-P-SYN-RXN RO:0002234 gomodel:CHEBI_29888_THI-P-SYN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/24; endpoint types: /individuals/36/type; /individuals/11/type
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		has_output

ADP(3-)
CHEBI:456216
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN RO:0002234 gomodel:CHEBI_456216_OHMETPYRKIN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/26; endpoint types: /individuals/30/type; /individuals/19/type
thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THI-P-SYN-RXN BFO:0000066 gomodel:reaction_THI-P-SYN-RXN_location_lociGO_0005829.

    Source location./YeastPathways_PWY3O-17.json#/facts/27; endpoint types: /individuals/36/type; /individuals/89/type
thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN		has_output

thiamine(1+) diphosphate(3-)
CHEBI:58937
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN RO:0002234 gomodel:CHEBI_58937_THIAMIN-PYROPHOSPHOKINASE-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/28; endpoint types: /individuals/37/type; /individuals/27/type
thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN		has_input

hydron
CHEBI:15378
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THI-P-SYN-RXN RO:0002233 gomodel:CHEBI_15378_THI-P-SYN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/29; endpoint types: /individuals/36/type; /individuals/5/type
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		has_output

ADP(3-)
CHEBI:456216
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 RO:0002234 gomodel:YeastPathways_PWY3O-17/6a4c244800007662.

    Source location./YeastPathways_PWY3O-17.json#/facts/30; endpoint types: /individuals/103/type; /individuals/105/type
phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:PYRIMSYN3-RXN BFO:0000066 gomodel:reaction_PYRIMSYN3-RXN_location_lociGO_0005829.

    Source location./YeastPathways_PWY3O-17.json#/facts/31; endpoint types: /individuals/31/type; /individuals/84/type
thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN		has_input

thiamine(1+)
CHEBI:18385
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN RO:0002233 gomodel:CHEBI_18385_RXNQT-4191.

    Source location./YeastPathways_PWY3O-17.json#/facts/32; endpoint types: /individuals/37/type; /individuals/10/type
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		has_output

hydron
CHEBI:15378
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 RO:0002234 gomodel:YeastPathways_PWY3O-17/6a4c244800007663.

    Source location./YeastPathways_PWY3O-17.json#/facts/33; endpoint types: /individuals/103/type; /individuals/106/type
THI21 Scer
SGD:S000006179		enables

phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007677 RO:0002333 gomodel:YeastPathways_PWY3O-17/6a4c244800007680.

    Source location./YeastPathways_PWY3O-17.json#/facts/34; endpoint types: /individuals/119/type; /individuals/122/type
thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN		has_input

4-methyl-5-(2-phosphonatooxyethyl)thiazole(2-)
CHEBI:58296
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THI-P-SYN-RXN RO:0002233 gomodel:CHEBI_58296_RXNQT-4301.

    Source location./YeastPathways_PWY3O-17.json#/facts/36; endpoint types: /individuals/36/type; /individuals/25/type
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		has_input

ATP(4-)
CHEBI:30616
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN RO:0002233 gomodel:CHEBI_30616_OHMETPYRKIN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/38; endpoint types: /individuals/30/type; /individuals/12/type
phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677		has_output

4-amino-2-methyl-5-diphosphonatooxymethylpyrimidine(3-)
CHEBI:57841
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007677 RO:0002234 gomodel:CHEBI_57841_PYRIMSYN3-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/39; endpoint types: /individuals/119/type; /individuals/24/type
phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677		has_input

ATP(4-)
CHEBI:30616
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007677 RO:0002233 gomodel:YeastPathways_PWY3O-17/6a4c244800007678.

    Source location./YeastPathways_PWY3O-17.json#/facts/40; endpoint types: /individuals/119/type; /individuals/120/type
THI80 Scer
SGD:S000005669		enables

thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN RO:0002333 gomodel:YeastPathways_PWY3O-17/6690711d00000657.

    Source location./YeastPathways_PWY3O-17.json#/facts/41; endpoint types: /individuals/37/type; /individuals/142/type
thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN		has_output

adenosine 5'-monophosphate(2-)
CHEBI:456215
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN RO:0002234 gomodel:CHEBI_456215_THIAMIN-PYROPHOSPHOKINASE-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/42; endpoint types: /individuals/37/type; /individuals/18/type
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
gomodel:RXN3O-9804		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXN3O-9804 BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/43; endpoint types: /individuals/33/type; /individuals/91/type
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXN3O-401 BFO:0000066 gomodel:reaction_RXN3O-401_location_lociGO_0005829.

    Source location./YeastPathways_PWY3O-17.json#/facts/44; endpoint types: /individuals/32/type; /individuals/85/type
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		provides_input_for

ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXN3O-401 RO:0002413 gomodel:RXNQT-4301.

    Source location./YeastPathways_PWY3O-17.json#/facts/45; endpoint types: /individuals/32/type; /individuals/35/type
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN BFO:0000066 gomodel:reaction_OHMETPYRKIN-RXN_location_lociGO_0005829.

    Source location./YeastPathways_PWY3O-17.json#/facts/46; endpoint types: /individuals/30/type; /individuals/83/type
thiamine phosphate phosphatase activity
gomodel:RXNQT-4191		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4191 BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/47; endpoint types: /individuals/34/type; /individuals/91/type
phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007677 BFO:0000066 gomodel:YeastPathways_PWY3O-17/6a4c244800007682.

    Source location./YeastPathways_PWY3O-17.json#/facts/48; endpoint types: /individuals/119/type; /individuals/123/type
phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN		has_input

4-amino-2-methyl-5-phosphonatooxymethylpyrimidine(2-)
CHEBI:58354
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:PYRIMSYN3-RXN RO:0002233 gomodel:CHEBI_58354_OHMETPYRKIN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/49; endpoint types: /individuals/31/type; /individuals/26/type
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4301 BFO:0000066 gomodel:reaction_RXNQT-4301_location_lociGO_0005829.

    Source location./YeastPathways_PWY3O-17.json#/facts/50; endpoint types: /individuals/35/type; /individuals/88/type
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		has_output

4-amino-2-methyl-5-phosphonatooxymethylpyrimidine(2-)
CHEBI:58354
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 RO:0002234 gomodel:CHEBI_58354_OHMETPYRKIN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/51; endpoint types: /individuals/103/type; /individuals/26/type
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		has_output

Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.

hydron
CHEBI:15378
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4301 RO:0002234 gomodel:CHEBI_15378_RXNQT-4301.

    Source location./YeastPathways_PWY3O-17.json#/facts/52; endpoint types: /individuals/35/type; /individuals/4/type
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		has_output

hydron
CHEBI:15378
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN RO:0002234 gomodel:CHEBI_15378_OHMETPYRKIN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/53; endpoint types: /individuals/30/type; /individuals/3/type
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		provides_input_for

thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4301 RO:0002413 gomodel:THI-P-SYN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/54; endpoint types: /individuals/35/type; /individuals/36/type
phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677		provides_input_for

thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007677 RO:0002413 gomodel:THI-P-SYN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/56; endpoint types: /individuals/119/type; /individuals/36/type
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 BFO:0000066 gomodel:YeastPathways_PWY3O-17/6a4c244800007666.

    Source location./YeastPathways_PWY3O-17.json#/facts/57; endpoint types: /individuals/103/type; /individuals/108/type
thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN		has_input

ATP(4-)
CHEBI:30616
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN RO:0002233 gomodel:CHEBI_30616_THIAMIN-PYROPHOSPHOKINASE-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/58; endpoint types: /individuals/37/type; /individuals/14/type
thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN		has_output

hydron
CHEBI:15378
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN RO:0002234 gomodel:CHEBI_15378_THIAMIN-PYROPHOSPHOKINASE-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/59; endpoint types: /individuals/37/type; /individuals/6/type
thiamine phosphate phosphatase activity
gomodel:RXNQT-4191		provides_input_for

thiamine diphosphokinase activity
gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4191 RO:0002413 gomodel:THIAMIN-PYROPHOSPHOKINASE-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/61; endpoint types: /individuals/34/type; /individuals/37/type
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/62; endpoint types: /individuals/103/type; /individuals/91/type
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		has_output

4-amino-2-methyl-5-phosphonatooxymethylpyrimidine(2-)
CHEBI:58354
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN RO:0002234 gomodel:CHEBI_58354_OHMETPYRKIN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/63; endpoint types: /individuals/30/type; /individuals/26/type
phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN		provides_input_for

thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:PYRIMSYN3-RXN RO:0002413 gomodel:THI-P-SYN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/64; endpoint types: /individuals/31/type; /individuals/36/type
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/65; endpoint types: /individuals/30/type; /individuals/91/type
THI6 Scer
SGD:S000006135		enables

thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THI-P-SYN-RXN RO:0002333 gomodel:YeastPathways_PWY3O-17/6690711d00000655.

    Source location./YeastPathways_PWY3O-17.json#/facts/66; endpoint types: /individuals/36/type; /individuals/140/type
phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN		has_output

4-amino-2-methyl-5-diphosphonatooxymethylpyrimidine(3-)
CHEBI:57841
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:PYRIMSYN3-RXN RO:0002234 gomodel:CHEBI_57841_PYRIMSYN3-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/67; endpoint types: /individuals/31/type; /individuals/24/type
phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007677 BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/68; endpoint types: /individuals/119/type; /individuals/91/type
thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN		has_output

thiamine(1+) monophosphate(2-)
CHEBI:37575
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THI-P-SYN-RXN RO:0002234 gomodel:CHEBI_37575_THI-P-SYN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/70; endpoint types: /individuals/36/type; /individuals/15/type
ADP-5-ethyl-4-methylthiazole-2-carboxylate + H2O -> AMP + 4-methyl-5-(2-phosphooxyethyl)thiazole + CO2 + H+
gomodel:RXNQT-4301		has_input

Native model bridge from ADP-thiazole to thiazole phosphate; the physiological enzyme assignment remains unresolved. THI4 is not assigned to this hydrolysis/decarboxylation step.

water
CHEBI:15377
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4301 RO:0002233 gomodel:CHEBI_15377_RXNQT-4301.

    Source location./YeastPathways_PWY3O-17.json#/facts/72; endpoint types: /individuals/35/type; /individuals/2/type
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		provides_input_for

phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN RO:0002413 gomodel:PYRIMSYN3-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/73; endpoint types: /individuals/30/type; /individuals/31/type
THI20 Scer
SGD:S000005416		enables

hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN RO:0002333 gomodel:YeastPathways_PWY3O-17/6690711d00000645.

    Source location./YeastPathways_PWY3O-17.json#/facts/74; endpoint types: /individuals/30/type; /individuals/134/type
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		provides_input_for

phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 RO:0002413 gomodel:YeastPathways_PWY3O-17/6a4c244800007677.

    Source location./YeastPathways_PWY3O-17.json#/facts/75; endpoint types: /individuals/103/type; /individuals/119/type
thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN		has_input

4-amino-2-methyl-5-diphosphonatooxymethylpyrimidine(3-)
CHEBI:57841
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THI-P-SYN-RXN RO:0002233 gomodel:CHEBI_57841_PYRIMSYN3-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/78; endpoint types: /individuals/36/type; /individuals/24/type
phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677		has_input

4-amino-2-methyl-5-phosphonatooxymethylpyrimidine(2-)
CHEBI:58354
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007677 RO:0002233 gomodel:CHEBI_58354_OHMETPYRKIN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/79; endpoint types: /individuals/119/type; /individuals/26/type
hydroxymethylpyrimidine kinase activity
gomodel:OHMETPYRKIN-RXN		has_input

4-amino-5-hydroxymethyl-2-methylpyrimidine
CHEBI:16892
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:OHMETPYRKIN-RXN RO:0002233 gomodel:CHEBI_16892_RXN3O-9804.

    Source location./YeastPathways_PWY3O-17.json#/facts/80; endpoint types: /individuals/30/type; /individuals/8/type
THI4 Scer
SGD:S000003376		enables

cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXN3O-401 RO:0002333 gomodel:YeastPathways_PWY3O-17/6690711d00000653.

    Source location./YeastPathways_PWY3O-17.json#/facts/81; endpoint types: /individuals/32/type; /individuals/138/type
phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN		part_of

thiamine biosynthetic process
GO:0009228
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:PYRIMSYN3-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-17/YeastPathways_PWY3O-17.

    Source location./YeastPathways_PWY3O-17.json#/facts/82; endpoint types: /individuals/31/type; /individuals/91/type
thiamine phosphate phosphatase activity
gomodel:RXNQT-4191		occurs_in

cytosol
GO:0005829
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXNQT-4191 BFO:0000066 gomodel:reaction_RXNQT-4191_location_lociGO_0005829.

    Source location./YeastPathways_PWY3O-17.json#/facts/83; endpoint types: /individuals/34/type; /individuals/87/type
hydroxymethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007660		provides_input_for

phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007660 RO:0002413 gomodel:PYRIMSYN3-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/84; endpoint types: /individuals/103/type; /individuals/31/type
THI13 Scer
SGD:S000002403		enables

Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.

4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
gomodel:RXN3O-9804
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:RXN3O-9804 RO:0002333 gomodel:MONOMER3O-9145_RXN3O-9804_controller.

    Source location./YeastPathways_PWY3O-17.json#/facts/85; endpoint types: /individuals/33/type; /individuals/29/type
  • UniProtKB:Q07748

    Source assertion: THI13 uses its active-site histidine and protein-bound PLP to form HMP-phosphate in a single turnover, generating inactive enzyme; this annotation is inferred by similarity to THI5.

    Source locationhttps://rest.uniprot.org/uniprotkb/Q07748.json [sha256:3b3a42882440ce399581bed512ee732900b8883a786945a0ffa216a8b33a7755] #/comments/0/texts/0
thiamine-phosphate diphosphorylase activity
gomodel:THI-P-SYN-RXN		provides_input_for

thiamine phosphate phosphatase activity
gomodel:RXNQT-4191
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:THI-P-SYN-RXN RO:0002413 gomodel:RXNQT-4191.

    Source location./YeastPathways_PWY3O-17.json#/facts/86; endpoint types: /individuals/36/type; /individuals/34/type
THI4 Scer
SGD:S000003376		located_in

Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:15544818); ECO:0000269 (PubMed:16850348) Source qualification: Excluded from the vacuole

cytoplasm
GO:0005737
  • UniProtKB:P32318

    Source assertion: UniProtKB:P32318 annotates location Cytoplasm (SL-0086).

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/861/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d
  • UniProtKB:P32318

    Source assertion: UniProtKB:P32318 has the exact SGD cross-reference SGD:S000003376.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/861/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d
THI4 Scer
SGD:S000003376		located_in

Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:16850348) Source qualification: Excluded from the vacuole

nucleus
GO:0005634
  • UniProtKB:P32318

    Source assertion: UniProtKB:P32318 annotates location Nucleus (SL-0191).

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/861/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d
  • UniProtKB:P32318

    Source assertion: UniProtKB:P32318 has the exact SGD cross-reference SGD:S000003376.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/861/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
gomodel:RXN3O-9804		has_input

Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.

N(6)-(pyridoxal phosphate)-L-lysine(2-) residue
CHEBI:143915
  • UniProtKB:Q07748

    Source assertion: The THI13 annotation, inferred by similarity to THI5, uses protein-bound PLP-lysine and histidine residues and forms HMP-phosphate. This bounded projection retains those core endpoints of the source equation, without asserting its exact net stoichiometry or residue and small-molecule products.

    Source locationhttps://rest.uniprot.org/uniprotkb/Q07748.json [sha256:3b3a42882440ce399581bed512ee732900b8883a786945a0ffa216a8b33a7755] #/comments/1/reaction
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
gomodel:RXN3O-9804		has_input

Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.

L-histidine residue
CHEBI:29979
  • UniProtKB:Q07748

    Source assertion: The THI13 annotation, inferred by similarity to THI5, uses protein-bound PLP-lysine and histidine residues and forms HMP-phosphate. This bounded projection retains those core endpoints of the source equation, without asserting its exact net stoichiometry or residue and small-molecule products.

    Source locationhttps://rest.uniprot.org/uniprotkb/Q07748.json [sha256:3b3a42882440ce399581bed512ee732900b8883a786945a0ffa216a8b33a7755] #/comments/1/reaction
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
gomodel:RXN3O-9804		has_output

Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.

4-amino-2-methyl-5-phosphonatooxymethylpyrimidine(2-)
CHEBI:58354
  • UniProtKB:Q07748

    Source assertion: The THI13 annotation, inferred by similarity to THI5, uses protein-bound PLP-lysine and histidine residues and forms HMP-phosphate. This bounded projection retains those core endpoints of the source equation, without asserting its exact net stoichiometry or residue and small-molecule products.

    Source locationhttps://rest.uniprot.org/uniprotkb/Q07748.json [sha256:3b3a42882440ce399581bed512ee732900b8883a786945a0ffa216a8b33a7755] #/comments/1/reaction
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
gomodel:RXN3O-9804		provides_input_for

Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.

phosphomethylpyrimidine kinase activity
gomodel:PYRIMSYN3-RXN
  • UniProtKB:Q07748

    Source assertion: THI13 has HMP-phosphate as a product; it supplies the substrate of the source model's phosphomethylpyrimidine kinase activities.

    Source locationhttps://rest.uniprot.org/uniprotkb/Q07748.json [sha256:3b3a42882440ce399581bed512ee732900b8883a786945a0ffa216a8b33a7755] #/comments/1/reaction
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:PYRIMSYN3-RXN RO:0002233 gomodel:CHEBI_58354_OHMETPYRKIN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/49; endpoint types: /individuals/31/type; /individuals/26/type
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase activity from histidine and PLP
gomodel:RXN3O-9804		provides_input_for

Single-turnover THI13 chemistry inferred by similarity to THI5 (ECO:0000250, UniProtKB:P43534). Histidine and PLP-lysine are protein-bound residues, not free histidine substrates. HMP-phosphate is the product. The inherited exact iron redox, water/proton balance, 3-oxopropanoate and residue-product assertions are quarantined: PMID:35675507 reports Fe(II)/oxygen dependence and different PLP-derived products for Candida albicans THI5, and a histidine-derived alpha-ketoacid for both Candida THI5 and S. cerevisiae THI5. These homolog experiments are not direct evidence for S. cerevisiae THI13; no replacement oxygen or product edges, or complete net stoichiometry, are inferred. Omitted products and balancing species remain unresolved, rather than asserted absent.

phosphomethylpyrimidine kinase activity
gomodel:YeastPathways_PWY3O-17/6a4c244800007677
  • UniProtKB:Q07748

    Source assertion: THI13 has HMP-phosphate as a product; it supplies the substrate of the source model's phosphomethylpyrimidine kinase activities.

    Source locationhttps://rest.uniprot.org/uniprotkb/Q07748.json [sha256:3b3a42882440ce399581bed512ee732900b8883a786945a0ffa216a8b33a7755] #/comments/1/reaction
  • gomodel:YeastPathways_PWY3O-17

    Source assertion: The model asserts gomodel:YeastPathways_PWY3O-17/6a4c244800007677 RO:0002233 gomodel:CHEBI_58354_OHMETPYRKIN-RXN.

    Source location./YeastPathways_PWY3O-17.json#/facts/79; endpoint types: /individuals/119/type; /individuals/26/type
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		has_input

THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.

L-cysteine residue
CHEBI:29950
  • UniProtKB:P32318

    Source assertion: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)

    Source locationhttps://rest.uniprot.org/uniprotkb/P32318.json [sha256:c07f0cf9365410e43a34cc0f50d55b265f76a045e0dddb5821c6bb3c9120ec60] #/comments/1/reaction
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		has_input

THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.

glycine zwitterion
CHEBI:57305
  • UniProtKB:P32318

    Source assertion: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)

    Source locationhttps://rest.uniprot.org/uniprotkb/P32318.json [sha256:c07f0cf9365410e43a34cc0f50d55b265f76a045e0dddb5821c6bb3c9120ec60] #/comments/1/reaction
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		has_input

THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.

NAD(1-)
CHEBI:57540
  • UniProtKB:P32318

    Source assertion: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)

    Source locationhttps://rest.uniprot.org/uniprotkb/P32318.json [sha256:c07f0cf9365410e43a34cc0f50d55b265f76a045e0dddb5821c6bb3c9120ec60] #/comments/1/reaction
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		has_output

THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.

dehydroalanine residue
CHEBI:90873
  • UniProtKB:P32318

    Source assertion: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)

    Source locationhttps://rest.uniprot.org/uniprotkb/P32318.json [sha256:c07f0cf9365410e43a34cc0f50d55b265f76a045e0dddb5821c6bb3c9120ec60] #/comments/1/reaction
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		has_output

THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.

ADP-5-ethyl-4-methylthiazole-2-carboxylate(3-)
CHEBI:139151
  • UniProtKB:P32318

    Source assertion: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)

    Source locationhttps://rest.uniprot.org/uniprotkb/P32318.json [sha256:c07f0cf9365410e43a34cc0f50d55b265f76a045e0dddb5821c6bb3c9120ec60] #/comments/1/reaction
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		has_output

THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.

nicotinamide
CHEBI:17154
  • UniProtKB:P32318

    Source assertion: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)

    Source locationhttps://rest.uniprot.org/uniprotkb/P32318.json [sha256:c07f0cf9365410e43a34cc0f50d55b265f76a045e0dddb5821c6bb3c9120ec60] #/comments/1/reaction
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		has_output

THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.

water
CHEBI:15377
  • UniProtKB:P32318

    Source assertion: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)

    Source locationhttps://rest.uniprot.org/uniprotkb/P32318.json [sha256:c07f0cf9365410e43a34cc0f50d55b265f76a045e0dddb5821c6bb3c9120ec60] #/comments/1/reaction
cysteine-dependent adenosine diphosphate thiazole synthase activity
gomodel:RXN3O-401		has_output

THI4 forms ADP-thiazole using its own cysteine residue as sulfur donor, leaving a dehydroalanine residue after one turnover. The reviewed equation produces three waters and two H+; it does not identify THI4 as the enzyme for downstream ADP-thiazole hydrolysis.

hydron
CHEBI:15378
  • UniProtKB:P32318

    Source assertion: [ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)

    Source locationhttps://rest.uniprot.org/uniprotkb/P32318.json [sha256:c07f0cf9365410e43a34cc0f50d55b265f76a045e0dddb5821c6bb3c9120ec60] #/comments/1/reaction
THI4 Scer
SGD:S000003376		has_cofactor

UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:22031445) Binds 1 Fe cation per subunit. UniProt note evidence: ECO:0000269 (PubMed:22031445)

iron cation
CHEBI:24875
  • UniProtKB:P32318

    Source assertion: UniProtKB:P32318 annotates CHEBI:24875 as a cofactor of this protein.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/861/comments/0/cofactors/0; /results/861/comments/0/note
  • UniProtKB:P32318

    Source assertion: UniProtKB:P32318 has the exact SGD cross-reference SGD:S000003376.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/861/uniProtKBCrossReferences/0
THI6 Scer
SGD:S000006135		has_cofactor

UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 Mg(2+) ion per subunit. UniProt note evidence: ECO:0000250

magnesium(2+)
CHEBI:18420
  • UniProtKB:P41835

    Source assertion: UniProtKB:P41835 annotates CHEBI:18420 as a cofactor of this protein.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1822/comments/0/cofactors/0; /results/1822/comments/0/note
  • UniProtKB:P41835

    Source assertion: UniProtKB:P41835 has the exact SGD cross-reference SGD:S000006135.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1822/uniProtKBCrossReferences/0
THI13 Scer
SGD:S000002403		has_cofactor

UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P43534)

iron cation
CHEBI:24875
  • UniProtKB:Q07748

    Source assertion: UniProtKB:Q07748 annotates CHEBI:24875 as a cofactor of this protein.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4991/comments/0/cofactors/0
  • UniProtKB:Q07748

    Source assertion: UniProtKB:Q07748 has the exact SGD cross-reference SGD:S000002403.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/4991/uniProtKBCrossReferences/0

References

Curation history