palmitoleate biosynthesis

gomodel:YeastPathways_PWY3O-1801

Palmitoleate biosynthesis in Saccharomyces cerevisiae S288C uses Ole1p to desaturate palmitoyl-CoA to palmitoleoyl-CoA and Tes1p to hydrolyze palmitoleoyl-CoA to palmitoleate and coenzyme A.

Pathway type

fatty acid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

18 nodes · 20 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
water `CHEBI:15377`	small molecule
unsaturated fatty acid biosynthetic process `GO:0006636`	biological process
palmitoleoyl-CoA(4-) `CHEBI:61540`	small molecule
hydron `CHEBI:15378`	small molecule
a ferricytochrome b5 `gomodel:FERRICYTOCHROME-B5_RXN-10664`	protein
coenzyme A(4-) `CHEBI:57287`	small molecule
cytosol `GO:0005829`	cellular component
a ferrocytochrome b5 `gomodel:FERROCYTOCHROME-B5_RXN-10664`	protein
palmitoyl-CoA(4-) `CHEBI:57379`	small molecule
palmitoleate `CHEBI:32372`	lipid
OLE1 Scer `SGD:S000003023`	protein
dioxygen `CHEBI:15379`	small molecule
TES1 Scer `SGD:S000003780`	protein
endoplasmic reticulum membrane `GO:0005789`	cellular component
peroxisome `GO:0005777`	cellular component
iron(2+) `CHEBI:29033`	cofactor
fatty acyl-CoA hydrolase activity `gomodel:RXN-10662`	molecular activity
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 RO:0002234 gomodel:CHEBI_15377_RXN-10664. Source location `./YeastPathways_PWY3O-1801.json#/facts/0; endpoint types: /individuals/12/type; /individuals/1/type`
fatty acyl-CoA hydrolase activity `gomodel:RXN-10662`	part_of	unsaturated fatty acid biosynthetic process `GO:0006636`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10662 BFO:0000050 gomodel:YeastPathways_PWY3O-1801/YeastPathways_PWY3O-1801. Source location `./YeastPathways_PWY3O-1801.json#/facts/1; endpoint types: /individuals/11/type; /individuals/31/type`
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	has_output	palmitoleoyl-CoA(4-) `CHEBI:61540`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 RO:0002234 gomodel:CHEBI_61540_RXN-10664. Source location `./YeastPathways_PWY3O-1801.json#/facts/2; endpoint types: /individuals/12/type; /individuals/8/type`
fatty acyl-CoA hydrolase activity `gomodel:RXN-10662`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10662 RO:0002234 gomodel:CHEBI_15378_RXN-10662. Source location `./YeastPathways_PWY3O-1801.json#/facts/3; endpoint types: /individuals/11/type; /individuals/2/type`
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	has_output	a ferricytochrome b5 `gomodel:FERRICYTOCHROME-B5_RXN-10664`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 RO:0002234 gomodel:FERRICYTOCHROME-B5_RXN-10664. Source location `./YeastPathways_PWY3O-1801.json#/facts/4; endpoint types: /individuals/12/type; /individuals/9/type`
fatty acyl-CoA hydrolase activity `gomodel:RXN-10662`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10662 RO:0002233 gomodel:CHEBI_15377_RXN-10662. Source location `./YeastPathways_PWY3O-1801.json#/facts/5; endpoint types: /individuals/11/type; /individuals/0/type`
fatty acyl-CoA hydrolase activity `gomodel:RXN-10662`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10662 RO:0002234 gomodel:CHEBI_57287_RXN-10662. Source location `./YeastPathways_PWY3O-1801.json#/facts/6; endpoint types: /individuals/11/type; /individuals/6/type`
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	provides_input_for	fatty acyl-CoA hydrolase activity `gomodel:RXN-10662`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 RO:0002413 gomodel:RXN-10662. Source location `./YeastPathways_PWY3O-1801.json#/facts/7; endpoint types: /individuals/12/type; /individuals/11/type`
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	has_input	a ferrocytochrome b5 `gomodel:FERROCYTOCHROME-B5_RXN-10664`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 RO:0002233 gomodel:FERROCYTOCHROME-B5_RXN-10664. Source location `./YeastPathways_PWY3O-1801.json#/facts/9; endpoint types: /individuals/12/type; /individuals/10/type`
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	has_input	palmitoyl-CoA(4-) `CHEBI:57379`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 RO:0002233 gomodel:CHEBI_57379_RXN-10664. Source location `./YeastPathways_PWY3O-1801.json#/facts/10; endpoint types: /individuals/12/type; /individuals/7/type`
fatty acyl-CoA hydrolase activity `gomodel:RXN-10662`	has_output	palmitoleate `CHEBI:32372`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10662 RO:0002234 gomodel:CHEBI_32372_RXN-10662. Source location `./YeastPathways_PWY3O-1801.json#/facts/12; endpoint types: /individuals/11/type; /individuals/5/type`
OLE1 Scer `SGD:S000003023`	enables	stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 RO:0002333 gomodel:YGL055W-MONOMER_RXN-10664_controller. Source location `./YeastPathways_PWY3O-1801.json#/facts/13; endpoint types: /individuals/12/type; /individuals/13/type`
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 RO:0002233 gomodel:CHEBI_15379_RXN-10664. Source location `./YeastPathways_PWY3O-1801.json#/facts/14; endpoint types: /individuals/12/type; /individuals/4/type`
fatty acyl-CoA hydrolase activity `gomodel:RXN-10662`	has_input	palmitoleoyl-CoA(4-) `CHEBI:61540`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10662 RO:0002233 gomodel:CHEBI_61540_RXN-10664. Source location `./YeastPathways_PWY3O-1801.json#/facts/15; endpoint types: /individuals/11/type; /individuals/8/type`
TES1 Scer `SGD:S000003780`	enables	fatty acyl-CoA hydrolase activity `gomodel:RXN-10662`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10662 RO:0002333 gomodel:YeastPathways_PWY3O-1801/6690711d00000541. Source location `./YeastPathways_PWY3O-1801.json#/facts/16; endpoint types: /individuals/11/type; /individuals/33/type`
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	part_of	unsaturated fatty acid biosynthetic process `GO:0006636`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 BFO:0000050 gomodel:YeastPathways_PWY3O-1801/YeastPathways_PWY3O-1801. Source location `./YeastPathways_PWY3O-1801.json#/facts/17; endpoint types: /individuals/12/type; /individuals/31/type`
stearoyl-CoA 9-desaturase activity `gomodel:RXN-10664`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-1801 Source assertion: The model asserts gomodel:RXN-10664 RO:0002233 gomodel:CHEBI_15378_RXN-10664. Source location `./YeastPathways_PWY3O-1801.json#/facts/18; endpoint types: /individuals/12/type; /individuals/3/type`
OLE1 Scer `SGD:S000003023`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:26928762) Topology: Multi-pass membrane protein.	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P21147 Source assertion: UniProtKB:P21147 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/539/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P21147 Source assertion: UniProtKB:P21147 has the exact SGD cross-reference SGD:S000003023. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/539/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
TES1 Scer `SGD:S000003780`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:10092594)	peroxisome `GO:0005777`	UniProtKB:P41903 Source assertion: UniProtKB:P41903 annotates location Peroxisome (SL-0204). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1826/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P41903 Source assertion: UniProtKB:P41903 has the exact SGD cross-reference SGD:S000003780. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1826/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
OLE1 Scer `SGD:S000003023`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P13516) Expected to bind 2 Fe(2+) ions per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P13516)	iron(2+) `CHEBI:29033`	UniProtKB:P21147 Source assertion: UniProtKB:P21147 annotates CHEBI:29033 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/539/comments/0/cofactors/0; /results/539/comments/0/note` UniProtKB:P21147 Source assertion: UniProtKB:P21147 has the exact SGD cross-reference SGD:S000003023. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/539/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWY3O-1801 GO-CAM palmitoleate biosynthesis model YeastPathways_PWY3O-1801 (source version: Pinned Noctua JSON archive; model date 2026-08-05; member ./YeastPathways_PWY3O-1801.json)
UniProtKB:P21147 Reviewed UniProtKB P21147 location annotations (source version: UniProt release 2026_03)
UniProtKB:P41903 Reviewed UniProtKB P41903 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 2 activity location assignments and 2 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 2 independently supported location edges and quarantined 2 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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