oleate biosynthesis

gomodel:YeastPathways_PWY3O-5268

Oleate biosynthesis in Saccharomyces cerevisiae S288C uses Ole1p to desaturate stearoyl-CoA to oleoyl-CoA and Tes1p to hydrolyze oleoyl-CoA to oleate and coenzyme A.

Pathway type
fatty acid biosynthesis
Organism scope

Read the source YAML

Pathway network

18 nodes · 19 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Pathway network: oleate biosynthesis 18 nodes · 19 asserted directed edges. Every arrow links to its evidence table row; nodes link to their component details. Isolated declared components are retained. SGD:S000003780 — enables → gomodel:RXN-9666; Evidence: gomodel:YeastPathways_PWY3O-5268enables gomodel:1.14.19.1-RXN — has_output → CHEBI:15377; Evidence: gomodel:YeastPathways_PWY3O-5268has output gomodel:1.14.19.1-RXN — has_input → CHEBI:57394; Evidence: gomodel:YeastPathways_PWY3O-5268has input gomodel:RXN-9666 — has_output → CHEBI:57287; Evidence: gomodel:YeastPathways_PWY3O-5268has output gomodel:1.14.19.1-RXN — has_input → CHEBI:15379; Evidence: gomodel:YeastPathways_PWY3O-5268has input gomodel:RXN-9666 — has_output → CHEBI:15378; Evidence: gomodel:YeastPathways_PWY3O-5268has output gomodel:RXN-9666 — part_of → GO:0006636; Evidence: gomodel:YeastPathways_PWY3O-5268part of gomodel:1.14.19.1-RXN — has_input → gomodel:FERROCYTOCHROME-B5_1.14.19.1-RXN; Evidence: gomodel:YeastPathways_PWY3O-5268has input gomodel:1.14.19.1-RXN — part_of → GO:0006636; Evidence: gomodel:YeastPathways_PWY3O-5268part of gomodel:1.14.19.1-RXN — has_input → CHEBI:15378; Evidence: gomodel:YeastPathways_PWY3O-5268has input gomodel:RXN-9666 — has_input → CHEBI:15377; Evidence: gomodel:YeastPathways_PWY3O-5268has input gomodel:RXN-9666 — has_output → CHEBI:30823; Evidence: gomodel:YeastPathways_PWY3O-5268has output SGD:S000003023 — enables → gomodel:1.14.19.1-RXN; Evidence: gomodel:YeastPathways_PWY3O-5268enables gomodel:1.14.19.1-RXN — has_output → gomodel:FERRICYTOCHROME-B5_1.14.19.1-RXN; Evidence: gomodel:YeastPathways_PWY3O-5268has output gomodel:RXN-9666 — has_input → CHEBI:57387; Evidence: gomodel:YeastPathways_PWY3O-5268has input gomodel:1.14.19.1-RXN — has_output → CHEBI:57387; Evidence: gomodel:YeastPathways_PWY3O-5268has output SGD:S000003780 — located_in → GO:0005777; Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:10092594); Evidence: UniProtKB:P41903, UniProtKB:P41903located in SGD:S000003023 — located_in → GO:0005789; Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:26928762) Topology: Multi-pass membrane protein.; Evidence: UniProtKB:P21147, UniProtKB:P21147located in SGD:S000003023 — has_cofactor → CHEBI:29033; UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P13516) Expected to bind 2 Fe(2+) ions per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P13516); Evidence: UniProtKB:P21147, UniProtKB:P21147has cofactor TES1 Scer; SGD:S000003780; Declared type: protein protein TES1 Scer water; CHEBI:15377; Declared type: small_molecule small molecule water stearoyl-CoA(4-); CHEBI:57394; Declared type: small_molecule small molecule stearoyl-CoA(4-) coenzyme A(4-); CHEBI:57287; Declared type: small_molecule small molecule coenzyme A(4-) dioxygen; CHEBI:15379; Declared type: small_molecule small molecule dioxygen hydron; CHEBI:15378; Declared type: small_molecule small molecule hydron cytosol; GO:0005829; Declared type: cellular_component cellular component cytosol unsaturated fatty acid biosynthetic process; GO:0006636; Declared type: biological_process biological process unsaturated fatty acid biosynthetic process a ferrocytochrome b5; gomodel:FERROCYTOCHROME-B5_1.14.19.1-RXN; Declared type: protein protein a ferrocytochrome b5 oleate; CHEBI:30823; Declared type: lipid lipid oleate OLE1 Scer; SGD:S000003023; Declared type: protein protein OLE1 Scer a ferricytochrome b5; gomodel:FERRICYTOCHROME-B5_1.14.19.1-RXN; Declared type: protein protein a ferricytochrome b5 oleoyl-CoA(4-); CHEBI:57387; Declared type: small_molecule small molecule oleoyl-CoA(4-) peroxisome; GO:0005777; Declared type: cellular_component cellular component peroxisome endoplasmic reticulum membrane; GO:0005789; Declared type: cellular_component cellular component endoplasmic reticulum membrane iron(2+); CHEBI:29033; Declared type: cofactor cofactor iron(2+) stearoyl-CoA 9-desaturase activity; gomodel:1.14.19.1-RXN; Declared type: molecular_activity molecular activity stearoyl-CoA 9-desaturase activity long-chain fatty acyl-CoA hydrolase activity; gomodel:RXN-9666; Declared type: molecular_activity molecular activity long-chain fatty acyl-CoA hydrolase activity

Components and activities

Declared biological components and source reaction directions
NodeKind or roleSource direction
Saccharomyces cerevisiae S288C
NCBITaxon:559292		taxon
TES1 Scer
SGD:S000003780		protein
water
CHEBI:15377		small molecule
stearoyl-CoA(4-)
CHEBI:57394		small molecule
coenzyme A(4-)
CHEBI:57287		small molecule
dioxygen
CHEBI:15379		small molecule
hydron
CHEBI:15378		small molecule
cytosol
GO:0005829		cellular component
unsaturated fatty acid biosynthetic process
GO:0006636		biological process
a ferrocytochrome b5
gomodel:FERROCYTOCHROME-B5_1.14.19.1-RXN		protein
oleate
CHEBI:30823		lipid
OLE1 Scer
SGD:S000003023		protein
a ferricytochrome b5
gomodel:FERRICYTOCHROME-B5_1.14.19.1-RXN		protein
oleoyl-CoA(4-)
CHEBI:57387		small molecule
peroxisome
GO:0005777		cellular component
endoplasmic reticulum membrane
GO:0005789		cellular component
iron(2+)
CHEBI:29033		cofactor
stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN		molecular activity
long-chain fatty acyl-CoA hydrolase activity
gomodel:RXN-9666		molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
SubjectPredicate and descriptionObjectEvidence
TES1 Scer
SGD:S000003780		enables

long-chain fatty acyl-CoA hydrolase activity
gomodel:RXN-9666
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:RXN-9666 RO:0002333 gomodel:YeastPathways_PWY3O-5268/67c10cc400000524.

    Source location./YeastPathways_PWY3O-5268.json#/facts/0; endpoint types: /individuals/11/type; /individuals/32/type
stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN		has_output

water
CHEBI:15377
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:1.14.19.1-RXN RO:0002234 gomodel:CHEBI_15377_1.14.19.1-RXN.

    Source location./YeastPathways_PWY3O-5268.json#/facts/1; endpoint types: /individuals/30/type; /individuals/0/type
stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN		has_input

stearoyl-CoA(4-)
CHEBI:57394
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:1.14.19.1-RXN RO:0002233 gomodel:CHEBI_57394_1.14.19.1-RXN.

    Source location./YeastPathways_PWY3O-5268.json#/facts/2; endpoint types: /individuals/30/type; /individuals/8/type
long-chain fatty acyl-CoA hydrolase activity
gomodel:RXN-9666		has_output

coenzyme A(4-)
CHEBI:57287
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:RXN-9666 RO:0002234 gomodel:CHEBI_57287_RXN-9666.

    Source location./YeastPathways_PWY3O-5268.json#/facts/3; endpoint types: /individuals/11/type; /individuals/6/type
stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN		has_input

dioxygen
CHEBI:15379
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:1.14.19.1-RXN RO:0002233 gomodel:CHEBI_15379_1.14.19.1-RXN.

    Source location./YeastPathways_PWY3O-5268.json#/facts/4; endpoint types: /individuals/30/type; /individuals/4/type
long-chain fatty acyl-CoA hydrolase activity
gomodel:RXN-9666		has_output

hydron
CHEBI:15378
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:RXN-9666 RO:0002234 gomodel:CHEBI_15378_RXN-9666.

    Source location./YeastPathways_PWY3O-5268.json#/facts/5; endpoint types: /individuals/11/type; /individuals/3/type
long-chain fatty acyl-CoA hydrolase activity
gomodel:RXN-9666		part_of

unsaturated fatty acid biosynthetic process
GO:0006636
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:RXN-9666 BFO:0000050 gomodel:YeastPathways_PWY3O-5268/YeastPathways_PWY3O-5268.

    Source location./YeastPathways_PWY3O-5268.json#/facts/7; endpoint types: /individuals/11/type; /individuals/31/type
stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN		has_input

a ferrocytochrome b5
gomodel:FERROCYTOCHROME-B5_1.14.19.1-RXN
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:1.14.19.1-RXN RO:0002233 gomodel:FERROCYTOCHROME-B5_1.14.19.1-RXN.

    Source location./YeastPathways_PWY3O-5268.json#/facts/8; endpoint types: /individuals/30/type; /individuals/10/type
stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN		part_of

unsaturated fatty acid biosynthetic process
GO:0006636
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:1.14.19.1-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-5268/YeastPathways_PWY3O-5268.

    Source location./YeastPathways_PWY3O-5268.json#/facts/9; endpoint types: /individuals/30/type; /individuals/31/type
stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN		has_input

hydron
CHEBI:15378
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:1.14.19.1-RXN RO:0002233 gomodel:CHEBI_15378_1.14.19.1-RXN.

    Source location./YeastPathways_PWY3O-5268.json#/facts/11; endpoint types: /individuals/30/type; /individuals/2/type
long-chain fatty acyl-CoA hydrolase activity
gomodel:RXN-9666		has_input

water
CHEBI:15377
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:RXN-9666 RO:0002233 gomodel:CHEBI_15377_RXN-9666.

    Source location./YeastPathways_PWY3O-5268.json#/facts/12; endpoint types: /individuals/11/type; /individuals/1/type
long-chain fatty acyl-CoA hydrolase activity
gomodel:RXN-9666		has_output

oleate
CHEBI:30823
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:RXN-9666 RO:0002234 gomodel:CHEBI_30823_RXN-9666.

    Source location./YeastPathways_PWY3O-5268.json#/facts/13; endpoint types: /individuals/11/type; /individuals/5/type
OLE1 Scer
SGD:S000003023		enables

stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:1.14.19.1-RXN RO:0002333 gomodel:YGL055W-MONOMER_1.14.19.1-RXN_controller.

    Source location./YeastPathways_PWY3O-5268.json#/facts/14; endpoint types: /individuals/30/type; /individuals/12/type
stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN		has_output

a ferricytochrome b5
gomodel:FERRICYTOCHROME-B5_1.14.19.1-RXN
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:1.14.19.1-RXN RO:0002234 gomodel:FERRICYTOCHROME-B5_1.14.19.1-RXN.

    Source location./YeastPathways_PWY3O-5268.json#/facts/15; endpoint types: /individuals/30/type; /individuals/9/type
long-chain fatty acyl-CoA hydrolase activity
gomodel:RXN-9666		has_input

oleoyl-CoA(4-)
CHEBI:57387
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:RXN-9666 RO:0002233 gomodel:CHEBI_57387_1.14.19.1-RXN.

    Source location./YeastPathways_PWY3O-5268.json#/facts/16; endpoint types: /individuals/11/type; /individuals/7/type
stearoyl-CoA 9-desaturase activity
gomodel:1.14.19.1-RXN		has_output

oleoyl-CoA(4-)
CHEBI:57387
  • gomodel:YeastPathways_PWY3O-5268

    Source assertion: The model asserts gomodel:1.14.19.1-RXN RO:0002234 gomodel:CHEBI_57387_1.14.19.1-RXN.

    Source location./YeastPathways_PWY3O-5268.json#/facts/17; endpoint types: /individuals/30/type; /individuals/7/type
TES1 Scer
SGD:S000003780		located_in

Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:10092594)

peroxisome
GO:0005777
  • UniProtKB:P41903

    Source assertion: UniProtKB:P41903 annotates location Peroxisome (SL-0204).

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1826/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d
  • UniProtKB:P41903

    Source assertion: UniProtKB:P41903 has the exact SGD cross-reference SGD:S000003780.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1826/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d
OLE1 Scer
SGD:S000003023		located_in

Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:26928762) Topology: Multi-pass membrane protein.

endoplasmic reticulum membrane
GO:0005789
  • UniProtKB:P21147

    Source assertion: UniProtKB:P21147 annotates location Endoplasmic reticulum membrane (SL-0097).

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/539/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d
  • UniProtKB:P21147

    Source assertion: UniProtKB:P21147 has the exact SGD cross-reference SGD:S000003023.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/539/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d
OLE1 Scer
SGD:S000003023		has_cofactor

UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P13516) Expected to bind 2 Fe(2+) ions per subunit. UniProt note evidence: ECO:0000250 (UniProtKB:P13516)

iron(2+)
CHEBI:29033
  • UniProtKB:P21147

    Source assertion: UniProtKB:P21147 annotates CHEBI:29033 as a cofactor of this protein.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/539/comments/0/cofactors/0; /results/539/comments/0/note
  • UniProtKB:P21147

    Source assertion: UniProtKB:P21147 has the exact SGD cross-reference SGD:S000003023.

    Source locationhttps://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/539/uniProtKBCrossReferences/0

References

Curation history