methionine salvage pathway

gomodel:YeastPathways_PWY3O-64

The methionine salvage pathway in Saccharomyces cerevisiae S288C recycles 5'-methylthioadenosine through methylthioribose phosphate, 4-methylthio-2-oxobutanoate, and L-methionine using the SGD GO-CAM MTA salvage reactions.

Pathway type

amino acid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

43 nodes · 82 asserted directed edges

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
dioxygen `CHEBI:15379`	small molecule
cytosol `GO:0005829`	cellular component
adenine `CHEBI:16708`	small molecule
L-methionine salvage `GO:0071267`	biological process
4-methylthio-2-oxobutanoate `CHEBI:16723`	small molecule
hydron `CHEBI:15378`	small molecule
ARO8 Scer `SGD:S000003170`	protein
2-oxo monocarboxylic acid `CHEBI:35910`	small molecule
5'-S-methyl-5'-thioadenosine `CHEBI:17509`	small molecule
L-methionine zwitterion `CHEBI:57844`	small molecule
proteinogenic amino acid `CHEBI:83813`	small molecule
formate `CHEBI:15740`	small molecule
MEU1 Scer `SGD:S000004007`	protein
S-methyl-5-thio-D-ribulose 1-phosphate(2-) `CHEBI:58548`	small molecule
water `CHEBI:15377`	small molecule
5-(methylsulfanyl)-2,3-dioxopentyl phosphate(2-) `CHEBI:58828`	small molecule
MDE1 Scer `SGD:S000003785`	protein
1-hydroxy-5-(methylthio)-3-oxopent-1-en-2-olate `CHEBI:58795`	small molecule
MRI1 Scer `SGD:S000006322`	protein
S-methyl-5-thio-alpha-D-ribose 1-phosphate(2-) `CHEBI:58533`	small molecule
hydrogenphosphate `CHEBI:43474`	small molecule
BAT1 Scer `SGD:S000001251`	protein
UTR4 Scer `SGD:S000000764`	protein
BAT2 Scer `SGD:S000003909`	protein
ARO9 Scer `SGD:S000001179`	protein
ADI1 Scer `SGD:S000004611`	protein
cytoplasm `GO:0005737`	cellular component
nucleus `GO:0005634`	cellular component
mitochondrial matrix `GO:0005759`	cellular component
pyridoxal 5'-phosphate(2-) `CHEBI:597326`	cofactor
zinc(2+) `CHEBI:29105`	cofactor
magnesium(2+) `CHEBI:18420`	cofactor
iron(2+) `CHEBI:29033`	cofactor
nickel(2+) `CHEBI:49786`	cofactor
5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O -> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate + H⁺ `gomodel:3.1.3.77-RXN`	molecular activity
S-methyl-5'-thioadenosine + phosphate -> adenine + S-methyl-5-thio-α-D-ribose 1-phosphate `gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN`	molecular activity
S-methyl-5-thio-α-D-ribose 1-phosphate -> 5-(methylsulfanyl)-ribulose 1-phosphate `gomodel:5.3.1.23-RXN`	molecular activity
5-(methylsulfanyl)-ribulose 1-phosphate -> 5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O `gomodel:R145-RXN`	molecular activity
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + oxygen -> 4-(methylsulfanyl)-2-oxobutanoate + formate + H⁺ `gomodel:R147-RXN`	molecular activity
L-methionine + a 2-oxo carboxylate <- 4-(methylsulfanyl)-2-oxobutanoate + a proteinogenic amino acid `gomodel:R15-RXN`	molecular activity
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001394`	molecular activity
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001410`	molecular activity
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001425`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + oxygen -> 4-(methylsulfanyl)-2-oxobutanoate + formate + H⁺ `gomodel:R147-RXN`	has_input	dioxygen `CHEBI:15379`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R147-RXN RO:0002233 gomodel:CHEBI_15379_R147-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/0; endpoint types: /individuals/23/type; /individuals/4/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001410`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001410 BFO:0000066 gomodel:YeastPathways_PWY3O-64/6a4c244800001417. Source location `./YeastPathways_PWY3O-64.json#/facts/1; endpoint types: /individuals/84/type; /individuals/89/type`
S-methyl-5'-thioadenosine + phosphate -> adenine + S-methyl-5-thio-α-D-ribose 1-phosphate `gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN`	has_output	adenine `CHEBI:16708`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN RO:0002234 gomodel:CHEBI_16708_5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/2; endpoint types: /individuals/67/type; /individuals/6/type`
S-methyl-5'-thioadenosine + phosphate -> adenine + S-methyl-5-thio-α-D-ribose 1-phosphate `gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN`	part_of	L-methionine salvage `GO:0071267`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-64/YeastPathways_PWY3O-64. Source location `./YeastPathways_PWY3O-64.json#/facts/3; endpoint types: /individuals/67/type; /individuals/69/type`
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + oxygen -> 4-(methylsulfanyl)-2-oxobutanoate + formate + H⁺ `gomodel:R147-RXN`	has_output	4-methylthio-2-oxobutanoate `CHEBI:16723`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R147-RXN RO:0002234 gomodel:CHEBI_16723_R147-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/4; endpoint types: /individuals/23/type; /individuals/7/type`
L-methionine + a 2-oxo carboxylate <- 4-(methylsulfanyl)-2-oxobutanoate + a proteinogenic amino acid `gomodel:R15-RXN`	has_input	4-methylthio-2-oxobutanoate `CHEBI:16723`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R15-RXN RO:0002233 gomodel:CHEBI_16723_R147-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/5; endpoint types: /individuals/24/type; /individuals/7/type`
5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O -> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate + H⁺ `gomodel:3.1.3.77-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:3.1.3.77-RXN RO:0002234 gomodel:CHEBI_15378_3.1.3.77-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/6; endpoint types: /individuals/66/type; /individuals/2/type`
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + oxygen -> 4-(methylsulfanyl)-2-oxobutanoate + formate + H⁺ `gomodel:R147-RXN`	part_of	L-methionine salvage `GO:0071267`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R147-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-64/YeastPathways_PWY3O-64. Source location `./YeastPathways_PWY3O-64.json#/facts/7; endpoint types: /individuals/23/type; /individuals/69/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001425`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001425 BFO:0000066 gomodel:YeastPathways_PWY3O-64/6a4c244800001431. Source location `./YeastPathways_PWY3O-64.json#/facts/8; endpoint types: /individuals/97/type; /individuals/102/type`
ARO8 Scer `SGD:S000003170`	enables	L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001410`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001410 RO:0002333 gomodel:YeastPathways_PWY3O-64/6a4c244800001414. Source location `./YeastPathways_PWY3O-64.json#/facts/9; endpoint types: /individuals/84/type; /individuals/88/type`
L-methionine + a 2-oxo carboxylate <- 4-(methylsulfanyl)-2-oxobutanoate + a proteinogenic amino acid `gomodel:R15-RXN`	has_output	2-oxo monocarboxylic acid `CHEBI:35910`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R15-RXN RO:0002234 gomodel:CHEBI_35910_R15-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/10; endpoint types: /individuals/24/type; /individuals/9/type`
S-methyl-5'-thioadenosine + phosphate -> adenine + S-methyl-5-thio-α-D-ribose 1-phosphate `gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN`	has_input	5'-S-methyl-5'-thioadenosine `CHEBI:17509`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN RO:0002233 gomodel:CHEBI_17509_5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/11; endpoint types: /individuals/67/type; /individuals/8/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001410`	has_output	L-methionine zwitterion `CHEBI:57844`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001410 RO:0002234 gomodel:YeastPathways_PWY3O-64/6a4c244800001413. Source location `./YeastPathways_PWY3O-64.json#/facts/12; endpoint types: /individuals/84/type; /individuals/87/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001394`	has_input	proteinogenic amino acid `CHEBI:83813`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001394 RO:0002233 gomodel:YeastPathways_PWY3O-64/6a4c244800001395. Source location `./YeastPathways_PWY3O-64.json#/facts/13; endpoint types: /individuals/71/type; /individuals/72/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001410`	has_output	2-oxo monocarboxylic acid `CHEBI:35910`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001410 RO:0002234 gomodel:YeastPathways_PWY3O-64/6a4c244800001412. Source location `./YeastPathways_PWY3O-64.json#/facts/14; endpoint types: /individuals/84/type; /individuals/86/type`
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + oxygen -> 4-(methylsulfanyl)-2-oxobutanoate + formate + H⁺ `gomodel:R147-RXN`	has_output	formate `CHEBI:15740`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R147-RXN RO:0002234 gomodel:CHEBI_15740_R147-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/15; endpoint types: /individuals/23/type; /individuals/5/type`
MEU1 Scer `SGD:S000004007`	enables	S-methyl-5'-thioadenosine + phosphate -> adenine + S-methyl-5-thio-α-D-ribose 1-phosphate `gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN RO:0002333 gomodel:MONOMER3O-157_5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN_controller. Source location `./YeastPathways_PWY3O-64.json#/facts/16; endpoint types: /individuals/67/type; /individuals/18/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001425`	has_output	L-methionine zwitterion `CHEBI:57844`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001425 RO:0002234 gomodel:YeastPathways_PWY3O-64/6a4c244800001428. Source location `./YeastPathways_PWY3O-64.json#/facts/17; endpoint types: /individuals/97/type; /individuals/100/type`
5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O -> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate + H⁺ `gomodel:3.1.3.77-RXN`	part_of	L-methionine salvage `GO:0071267`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:3.1.3.77-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-64/YeastPathways_PWY3O-64. Source location `./YeastPathways_PWY3O-64.json#/facts/18; endpoint types: /individuals/66/type; /individuals/69/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001425`	has_input	proteinogenic amino acid `CHEBI:83813`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001425 RO:0002233 gomodel:YeastPathways_PWY3O-64/6a4c244800001426. Source location `./YeastPathways_PWY3O-64.json#/facts/19; endpoint types: /individuals/97/type; /individuals/98/type`
5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O -> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate + H⁺ `gomodel:3.1.3.77-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:3.1.3.77-RXN BFO:0000066 gomodel:reaction_3.1.3.77-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-64.json#/facts/20; endpoint types: /individuals/66/type; /individuals/60/type`
5-(methylsulfanyl)-ribulose 1-phosphate -> 5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O `gomodel:R145-RXN`	has_input	S-methyl-5-thio-D-ribulose 1-phosphate(2-) `CHEBI:58548`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R145-RXN RO:0002233 gomodel:CHEBI_58548_5.3.1.23-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/21; endpoint types: /individuals/22/type; /individuals/14/type`
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + oxygen -> 4-(methylsulfanyl)-2-oxobutanoate + formate + H⁺ `gomodel:R147-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R147-RXN BFO:0000066 gomodel:reaction_R147-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-64.json#/facts/22; endpoint types: /individuals/23/type; /individuals/64/type`
5-(methylsulfanyl)-ribulose 1-phosphate -> 5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O `gomodel:R145-RXN`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R145-RXN RO:0002234 gomodel:CHEBI_15377_R145-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/23; endpoint types: /individuals/22/type; /individuals/1/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001410`	part_of	L-methionine salvage `GO:0071267`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001410 BFO:0000050 gomodel:YeastPathways_PWY3O-64/YeastPathways_PWY3O-64. Source location `./YeastPathways_PWY3O-64.json#/facts/24; endpoint types: /individuals/84/type; /individuals/69/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001425`	has_input	4-methylthio-2-oxobutanoate `CHEBI:16723`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001425 RO:0002233 gomodel:CHEBI_16723_R147-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/25; endpoint types: /individuals/97/type; /individuals/7/type`
5-(methylsulfanyl)-ribulose 1-phosphate -> 5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O `gomodel:R145-RXN`	part_of	L-methionine salvage `GO:0071267`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R145-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-64/YeastPathways_PWY3O-64. Source location `./YeastPathways_PWY3O-64.json#/facts/26; endpoint types: /individuals/22/type; /individuals/69/type`
L-methionine + a 2-oxo carboxylate <- 4-(methylsulfanyl)-2-oxobutanoate + a proteinogenic amino acid `gomodel:R15-RXN`	has_output	L-methionine zwitterion `CHEBI:57844`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R15-RXN RO:0002234 gomodel:CHEBI_57844_R15-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/27; endpoint types: /individuals/24/type; /individuals/12/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001410`	has_input	4-methylthio-2-oxobutanoate `CHEBI:16723`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001410 RO:0002233 gomodel:CHEBI_16723_R147-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/28; endpoint types: /individuals/84/type; /individuals/7/type`
5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O -> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate + H⁺ `gomodel:3.1.3.77-RXN`	has_input	5-(methylsulfanyl)-2,3-dioxopentyl phosphate(2-) `CHEBI:58828`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:3.1.3.77-RXN RO:0002233 gomodel:CHEBI_58828_R145-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/29; endpoint types: /individuals/66/type; /individuals/16/type`
L-methionine + a 2-oxo carboxylate <- 4-(methylsulfanyl)-2-oxobutanoate + a proteinogenic amino acid `gomodel:R15-RXN`	has_input	proteinogenic amino acid `CHEBI:83813`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R15-RXN RO:0002233 gomodel:CHEBI_83813_R15-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/30; endpoint types: /individuals/24/type; /individuals/17/type`
MDE1 Scer `SGD:S000003785`	enables	5-(methylsulfanyl)-ribulose 1-phosphate -> 5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O `gomodel:R145-RXN`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R145-RXN RO:0002333 gomodel:MONOMER3O-169_R145-RXN_controller. Source location `./YeastPathways_PWY3O-64.json#/facts/31; endpoint types: /individuals/22/type; /individuals/19/type`
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + oxygen -> 4-(methylsulfanyl)-2-oxobutanoate + formate + H⁺ `gomodel:R147-RXN`	has_input	1-hydroxy-5-(methylthio)-3-oxopent-1-en-2-olate `CHEBI:58795`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R147-RXN RO:0002233 gomodel:CHEBI_58795_3.1.3.77-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/32; endpoint types: /individuals/23/type; /individuals/15/type`
MRI1 Scer `SGD:S000006322`	enables	S-methyl-5-thio-α-D-ribose 1-phosphate -> 5-(methylsulfanyl)-ribulose 1-phosphate `gomodel:5.3.1.23-RXN`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5.3.1.23-RXN RO:0002333 gomodel:YeastPathways_PWY3O-64/6690711d00000278. Source location `./YeastPathways_PWY3O-64.json#/facts/33; endpoint types: /individuals/68/type; /individuals/110/type`
S-methyl-5-thio-α-D-ribose 1-phosphate -> 5-(methylsulfanyl)-ribulose 1-phosphate `gomodel:5.3.1.23-RXN`	has_output	S-methyl-5-thio-D-ribulose 1-phosphate(2-) `CHEBI:58548`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5.3.1.23-RXN RO:0002234 gomodel:CHEBI_58548_5.3.1.23-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/35; endpoint types: /individuals/68/type; /individuals/14/type`
5-(methylsulfanyl)-ribulose 1-phosphate -> 5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O `gomodel:R145-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R145-RXN BFO:0000066 gomodel:reaction_R145-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-64.json#/facts/36; endpoint types: /individuals/22/type; /individuals/63/type`
S-methyl-5'-thioadenosine + phosphate -> adenine + S-methyl-5-thio-α-D-ribose 1-phosphate `gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN`	has_output	S-methyl-5-thio-alpha-D-ribose 1-phosphate(2-) `CHEBI:58533`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN RO:0002234 gomodel:CHEBI_58533_5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/37; endpoint types: /individuals/67/type; /individuals/13/type`
5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O -> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate + H⁺ `gomodel:3.1.3.77-RXN`	has_output	1-hydroxy-5-(methylthio)-3-oxopent-1-en-2-olate `CHEBI:58795`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:3.1.3.77-RXN RO:0002234 gomodel:CHEBI_58795_3.1.3.77-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/38; endpoint types: /individuals/66/type; /individuals/15/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001394`	has_output	L-methionine zwitterion `CHEBI:57844`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001394 RO:0002234 gomodel:YeastPathways_PWY3O-64/6a4c244800001396. Source location `./YeastPathways_PWY3O-64.json#/facts/39; endpoint types: /individuals/71/type; /individuals/73/type`
S-methyl-5'-thioadenosine + phosphate -> adenine + S-methyl-5-thio-α-D-ribose 1-phosphate `gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN`	has_input	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN RO:0002233 gomodel:CHEBI_43474_5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/40; endpoint types: /individuals/67/type; /individuals/11/type`
S-methyl-5-thio-α-D-ribose 1-phosphate -> 5-(methylsulfanyl)-ribulose 1-phosphate `gomodel:5.3.1.23-RXN`	has_input	S-methyl-5-thio-alpha-D-ribose 1-phosphate(2-) `CHEBI:58533`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5.3.1.23-RXN RO:0002233 gomodel:CHEBI_58533_5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/41; endpoint types: /individuals/68/type; /individuals/13/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001394`	has_input	4-methylthio-2-oxobutanoate `CHEBI:16723`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001394 RO:0002233 gomodel:CHEBI_16723_R147-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/42; endpoint types: /individuals/71/type; /individuals/7/type`
BAT1 Scer `SGD:S000001251`	enables	L-methionine + a 2-oxo carboxylate <- 4-(methylsulfanyl)-2-oxobutanoate + a proteinogenic amino acid `gomodel:R15-RXN`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R15-RXN RO:0002333 gomodel:YHR208W-MONOMER_R15-RXN_controller. Source location `./YeastPathways_PWY3O-64.json#/facts/43; endpoint types: /individuals/24/type; /individuals/25/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001394`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001394 BFO:0000066 gomodel:YeastPathways_PWY3O-64/6a4c244800001402. Source location `./YeastPathways_PWY3O-64.json#/facts/44; endpoint types: /individuals/71/type; /individuals/76/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001425`	has_output	2-oxo monocarboxylic acid `CHEBI:35910`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001425 RO:0002234 gomodel:YeastPathways_PWY3O-64/6a4c244800001427. Source location `./YeastPathways_PWY3O-64.json#/facts/45; endpoint types: /individuals/97/type; /individuals/99/type`
5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O -> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate + H⁺ `gomodel:3.1.3.77-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:3.1.3.77-RXN RO:0002233 gomodel:CHEBI_15377_3.1.3.77-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/46; endpoint types: /individuals/66/type; /individuals/0/type`
UTR4 Scer `SGD:S000000764`	enables	5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O -> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate + H⁺ `gomodel:3.1.3.77-RXN`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:3.1.3.77-RXN RO:0002333 gomodel:MONOMER3O-175_3.1.3.77-RXN_controller. Source location `./YeastPathways_PWY3O-64.json#/facts/47; endpoint types: /individuals/66/type; /individuals/20/type`
S-methyl-5'-thioadenosine + phosphate -> adenine + S-methyl-5-thio-α-D-ribose 1-phosphate `gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN BFO:0000066 gomodel:reaction_5-METHYLTHIOADENOSINE-PHOSPHORYLASE-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-64.json#/facts/48; endpoint types: /individuals/67/type; /individuals/61/type`
5-(methylsulfanyl)-ribulose 1-phosphate -> 5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O `gomodel:R145-RXN`	has_output	5-(methylsulfanyl)-2,3-dioxopentyl phosphate(2-) `CHEBI:58828`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R145-RXN RO:0002234 gomodel:CHEBI_58828_R145-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/49; endpoint types: /individuals/22/type; /individuals/16/type`
BAT2 Scer `SGD:S000003909`	enables	L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001394`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001394 RO:0002333 gomodel:YeastPathways_PWY3O-64/6a4c244800001401. Source location `./YeastPathways_PWY3O-64.json#/facts/50; endpoint types: /individuals/71/type; /individuals/75/type`
S-methyl-5-thio-α-D-ribose 1-phosphate -> 5-(methylsulfanyl)-ribulose 1-phosphate `gomodel:5.3.1.23-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5.3.1.23-RXN BFO:0000066 gomodel:reaction_5.3.1.23-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-64.json#/facts/51; endpoint types: /individuals/68/type; /individuals/62/type`
L-methionine + a 2-oxo carboxylate <- 4-(methylsulfanyl)-2-oxobutanoate + a proteinogenic amino acid `gomodel:R15-RXN`	part_of	L-methionine salvage `GO:0071267`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R15-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-64/YeastPathways_PWY3O-64. Source location `./YeastPathways_PWY3O-64.json#/facts/52; endpoint types: /individuals/24/type; /individuals/69/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001425`	part_of	L-methionine salvage `GO:0071267`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001425 BFO:0000050 gomodel:YeastPathways_PWY3O-64/YeastPathways_PWY3O-64. Source location `./YeastPathways_PWY3O-64.json#/facts/53; endpoint types: /individuals/97/type; /individuals/69/type`
S-methyl-5-thio-α-D-ribose 1-phosphate -> 5-(methylsulfanyl)-ribulose 1-phosphate `gomodel:5.3.1.23-RXN`	part_of	L-methionine salvage `GO:0071267`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:5.3.1.23-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-64/YeastPathways_PWY3O-64. Source location `./YeastPathways_PWY3O-64.json#/facts/54; endpoint types: /individuals/68/type; /individuals/69/type`
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + oxygen -> 4-(methylsulfanyl)-2-oxobutanoate + formate + H⁺ `gomodel:R147-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R147-RXN RO:0002234 gomodel:CHEBI_15378_R147-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/55; endpoint types: /individuals/23/type; /individuals/3/type`
5-(methylsulfanyl)-2,3-dioxopentyl 1-phosphate + H₂O -> 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate + H⁺ `gomodel:3.1.3.77-RXN`	has_output	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:3.1.3.77-RXN RO:0002234 gomodel:CHEBI_43474_3.1.3.77-RXN. Source location `./YeastPathways_PWY3O-64.json#/facts/56; endpoint types: /individuals/66/type; /individuals/10/type`
ARO9 Scer `SGD:S000001179`	enables	L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001425`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001425 RO:0002333 gomodel:YeastPathways_PWY3O-64/6a4c244800001430. Source location `./YeastPathways_PWY3O-64.json#/facts/57; endpoint types: /individuals/97/type; /individuals/101/type`
ADI1 Scer `SGD:S000004611`	enables	1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + oxygen -> 4-(methylsulfanyl)-2-oxobutanoate + formate + H⁺ `gomodel:R147-RXN`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:R147-RXN RO:0002333 gomodel:MONOMER3O-186_R147-RXN_controller. Source location `./YeastPathways_PWY3O-64.json#/facts/58; endpoint types: /individuals/23/type; /individuals/21/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001394`	part_of	L-methionine salvage `GO:0071267`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001394 BFO:0000050 gomodel:YeastPathways_PWY3O-64/YeastPathways_PWY3O-64. Source location `./YeastPathways_PWY3O-64.json#/facts/59; endpoint types: /individuals/71/type; /individuals/69/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001394`	has_output	2-oxo monocarboxylic acid `CHEBI:35910`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001394 RO:0002234 gomodel:YeastPathways_PWY3O-64/6a4c244800001397. Source location `./YeastPathways_PWY3O-64.json#/facts/60; endpoint types: /individuals/71/type; /individuals/74/type`
L-isoleucine:2-oxoglutarate transaminase activity `gomodel:YeastPathways_PWY3O-64/6a4c244800001410`	has_input	proteinogenic amino acid `CHEBI:83813`	gomodel:YeastPathways_PWY3O-64 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-64/6a4c244800001410 RO:0002233 gomodel:YeastPathways_PWY3O-64/6a4c244800001411. Source location `./YeastPathways_PWY3O-64.json#/facts/61; endpoint types: /individuals/84/type; /individuals/85/type`
ARO8 Scer `SGD:S000003170`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P53090 Source assertion: UniProtKB:P53090 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2112/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P53090 Source assertion: UniProtKB:P53090 has the exact SGD cross-reference SGD:S000003170. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2112/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MEU1 Scer `SGD:S000004007`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	cytoplasm `GO:0005737`	UniProtKB:Q07938 Source assertion: UniProtKB:Q07938 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2845/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q07938 Source assertion: UniProtKB:Q07938 has the exact SGD cross-reference SGD:S000004007. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2845/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MEU1 Scer `SGD:S000004007`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	nucleus `GO:0005634`	UniProtKB:Q07938 Source assertion: UniProtKB:Q07938 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2845/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q07938 Source assertion: UniProtKB:Q07938 has the exact SGD cross-reference SGD:S000004007. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2845/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MDE1 Scer `SGD:S000003785`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03116); ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P47095 Source assertion: UniProtKB:P47095 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3872/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P47095 Source assertion: UniProtKB:P47095 has the exact SGD cross-reference SGD:S000003785. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3872/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MRI1 Scer `SGD:S000006322`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03119); ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:Q06489 Source assertion: UniProtKB:Q06489 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4195/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q06489 Source assertion: UniProtKB:Q06489 has the exact SGD cross-reference SGD:S000006322. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4195/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MRI1 Scer `SGD:S000006322`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03119); ECO:0000269 (PubMed:14562095)	nucleus `GO:0005634`	UniProtKB:Q06489 Source assertion: UniProtKB:Q06489 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4195/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q06489 Source assertion: UniProtKB:Q06489 has the exact SGD cross-reference SGD:S000006322. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/4195/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
BAT1 Scer `SGD:S000001251`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:21267457); ECO:0000269 (PubMed:8798704)	mitochondrial matrix `GO:0005759`	UniProtKB:P38891 Source assertion: UniProtKB:P38891 annotates location Mitochondrion matrix (SL-0170). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1497/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P38891 Source assertion: UniProtKB:P38891 has the exact SGD cross-reference SGD:S000001251. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1497/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
UTR4 Scer `SGD:S000000764`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03117); ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P32626 Source assertion: UniProtKB:P32626 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3436/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32626 Source assertion: UniProtKB:P32626 has the exact SGD cross-reference SGD:S000000764. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3436/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
UTR4 Scer `SGD:S000000764`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03117); ECO:0000269 (PubMed:14562095)	nucleus `GO:0005634`	UniProtKB:P32626 Source assertion: UniProtKB:P32626 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3436/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32626 Source assertion: UniProtKB:P32626 has the exact SGD cross-reference SGD:S000000764. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3436/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
BAT2 Scer `SGD:S000003909`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:21267457); ECO:0000269 (PubMed:8798704)	cytoplasm `GO:0005737`	UniProtKB:P47176 Source assertion: UniProtKB:P47176 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1987/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P47176 Source assertion: UniProtKB:P47176 has the exact SGD cross-reference SGD:S000003909. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1987/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ARO9 Scer `SGD:S000001179`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P38840 Source assertion: UniProtKB:P38840 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1469/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P38840 Source assertion: UniProtKB:P38840 has the exact SGD cross-reference SGD:S000001179. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1469/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ADI1 Scer `SGD:S000004611`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	cytoplasm `GO:0005737`	UniProtKB:Q03677 Source assertion: UniProtKB:Q03677 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2528/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q03677 Source assertion: UniProtKB:Q03677 has the exact SGD cross-reference SGD:S000004611. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2528/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ADI1 Scer `SGD:S000004611`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	nucleus `GO:0005634`	UniProtKB:Q03677 Source assertion: UniProtKB:Q03677 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2528/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q03677 Source assertion: UniProtKB:Q03677 has the exact SGD cross-reference SGD:S000004611. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2528/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ARO8 Scer `SGD:S000003170`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:23893908)	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P53090 Source assertion: UniProtKB:P53090 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2112/comments/0/cofactors/0` UniProtKB:P53090 Source assertion: UniProtKB:P53090 has the exact SGD cross-reference SGD:S000003170. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2112/uniProtKBCrossReferences/0`
MDE1 Scer `SGD:S000003785`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000255 (HAMAP-Rule:MF_03116) Binds 1 zinc ion per subunit. UniProt note evidence: ECO:0000255 (HAMAP-Rule:MF_03116)	zinc(2+) `CHEBI:29105`	UniProtKB:P47095 Source assertion: UniProtKB:P47095 annotates CHEBI:29105 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3872/comments/0/cofactors/0; /results/3872/comments/0/note` UniProtKB:P47095 Source assertion: UniProtKB:P47095 has the exact SGD cross-reference SGD:S000003785. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3872/uniProtKBCrossReferences/0`
BAT1 Scer `SGD:S000001251`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. Reviewed source qualification: Disputed growth phenotypes do not address PLP binding; retain the similarity-based PLP annotation. UniProt evidence: ECO:0000250 (UniProtKB:P0AB80)	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P38891 Source assertion: UniProtKB:P38891 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1497/comments/0/cofactors/0` UniProtKB:P38891 Source assertion: UniProtKB:P38891 has the exact SGD cross-reference SGD:S000001251. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1497/uniProtKBCrossReferences/0`
UTR4 Scer `SGD:S000000764`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified Binds 1 Mg(2+) ion per subunit.	magnesium(2+) `CHEBI:18420`	UniProtKB:P32626 Source assertion: UniProtKB:P32626 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3436/comments/0/cofactors/0; /results/3436/comments/0/note` UniProtKB:P32626 Source assertion: UniProtKB:P32626 has the exact SGD cross-reference SGD:S000000764. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3436/uniProtKBCrossReferences/0`
BAT2 Scer `SGD:S000003909`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P47176 Source assertion: UniProtKB:P47176 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1987/comments/0/cofactors/0` UniProtKB:P47176 Source assertion: UniProtKB:P47176 has the exact SGD cross-reference SGD:S000003909. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1987/uniProtKBCrossReferences/0`
ARO9 Scer `SGD:S000001179`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:O84395)	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P38840 Source assertion: UniProtKB:P38840 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1469/comments/0/cofactors/0` UniProtKB:P38840 Source assertion: UniProtKB:P38840 has the exact SGD cross-reference SGD:S000001179. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1469/uniProtKBCrossReferences/0`
ADI1 Scer `SGD:S000004611`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. Reviewed source qualification: ADI1 metal identity changes the chemical products. These are alternatives, not simultaneous requirements of one reaction. Iron-bound ADI1 supports the methionine-salvage branch producing 2-keto-4-methylthiobutyrate. UniProt evidence: ECO:0000255 (HAMAP-Rule:MF_03154) Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate. UniProt note evidence: ECO:0000255 (HAMAP-Rule:MF_03154)	iron(2+) `CHEBI:29033`	UniProtKB:Q03677 Source assertion: UniProtKB:Q03677 annotates CHEBI:29033 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2528/comments/0/cofactors/0; /results/2528/comments/0/note` UniProtKB:Q03677 Source assertion: UniProtKB:Q03677 has the exact SGD cross-reference SGD:S000004611. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2528/uniProtKBCrossReferences/0`
ADI1 Scer `SGD:S000004611`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. Reviewed source qualification: ADI1 metal identity changes the chemical products. These are alternatives, not simultaneous requirements of one reaction. Nickel-bound ADI1 supports an alternative branch producing 3-(methylsulfanyl)propanoate; this does not assert nickel is required for the methionine-salvage reaction. UniProt evidence: ECO:0000255 (HAMAP-Rule:MF_03154) Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate. UniProt note evidence: ECO:0000255 (HAMAP-Rule:MF_03154)	nickel(2+) `CHEBI:49786`	UniProtKB:Q03677 Source assertion: UniProtKB:Q03677 annotates CHEBI:49786 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2528/comments/0/cofactors/1; /results/2528/comments/0/note` UniProtKB:Q03677 Source assertion: UniProtKB:Q03677 has the exact SGD cross-reference SGD:S000004611. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2528/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWY3O-64 SGD GO-CAM methionine salvage pathway - imported from: Saccharomyces Genome Database (source version: Pinned Noctua JSON archive; model date 2026-07-13; member ./YeastPathways_PWY3O-64.json)
UniProtKB:P53090 Reviewed UniProtKB P53090 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q07938 Reviewed UniProtKB Q07938 location annotations (source version: UniProt release 2026_03)
UniProtKB:P47095 Reviewed UniProtKB P47095 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q06489 Reviewed UniProtKB Q06489 location annotations (source version: UniProt release 2026_03)
UniProtKB:P38891 Reviewed UniProtKB P38891 location annotations (source version: UniProt release 2026_03)
UniProtKB:P32626 Reviewed UniProtKB P32626 location annotations (source version: UniProt release 2026_03)
UniProtKB:P47176 Reviewed UniProtKB P47176 location annotations (source version: UniProt release 2026_03)
UniProtKB:P38840 Reviewed UniProtKB P38840 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q03677 Reviewed UniProtKB Q03677 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 9 activity location assignments and 9 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 13 independently supported location edges and quarantined 1 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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